Academic literature on the topic 'Cultures cellulaires – Biotechnologie'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Cultures cellulaires – Biotechnologie.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "Cultures cellulaires – Biotechnologie":
Roy, R., and J. Goulet. "Levains lactiques: corrélation entre les dénombrements sur gélose et l'ATP cellulaire." Canadian Journal of Microbiology 31, no. 6 (June 1, 1985): 555–57. http://dx.doi.org/10.1139/m85-103.
MALOBERTI, Alain. "Télécommunications cellulaires." Électronique, March 1987. http://dx.doi.org/10.51257/a-v1-e7785.
CELLMER, Jean. "Réseaux cellulaires - Introduction." Réseaux Télécommunications, February 1999. http://dx.doi.org/10.51257/a-v2-e7360.
CELLMER, Jean. "Réseaux cellulaires Système GSM." Réseaux Télécommunications, February 1999. http://dx.doi.org/10.51257/a-v1-e7364.
CELLMER, Jean. "Réseaux cellulaires - Radiocom 2000." Réseaux Télécommunications, February 1999. http://dx.doi.org/10.51257/a-v1-e7362.
CELLMER, Jean. "Réseaux cellulaires de radiocommunications mobiles." Réseaux Télécommunications, June 1994. http://dx.doi.org/10.51257/a-v1-e7360.
CELLMER, Jean. "Réseaux cellulaires Au-delà du GSM." Réseaux Télécommunications, February 1999. http://dx.doi.org/10.51257/a-v1-e7368.
CELLMER, Jean. "Réseaux cellulaires - Principes généraux. Systèmes analogiques." Réseaux Télécommunications, November 1998. http://dx.doi.org/10.51257/a-v1-e7361.
PONS, Jérôme. "Réseaux cellulaires - Évolution du système UMTS vers HSPA+." Réseaux Télécommunications, May 2011. http://dx.doi.org/10.51257/a-v1-te7370.
PONS, Jérôme. "Réseaux cellulaires - Évolution du système UMTS vers le système EPS." Réseaux Télécommunications, May 2011. http://dx.doi.org/10.51257/a-v1-te7371.
Dissertations / Theses on the topic "Cultures cellulaires – Biotechnologie":
Damerdji, Ouassila. "Etude de facteurs de croissance du lait bovin utilisation en biotechnologie, cultures cellulaires /." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37604188v.
André, Silvère. "Apports de la spectroscopie Raman et de la chimiométrie au suivi in situ de cultures cellulaires : nouvelles perspectives en biotechnologie." Thesis, Lille 1, 2016. http://www.theses.fr/2016LIL10142/document.
In the 2000s, the major sanitary safety authorities proposed the Process Analytical Technology initiative (PAT) encouraging pharmaceutical and agri-food industries to enhance their own processes and the control of manufacturing products by using new and innovative techniques. This thesis is directly related to this framework and proposes to use Raman spectroscopy, coupled to chemometric tools, to monitor cell cultures for pharmaceutical purposes. The in situ Raman spectra, acquired using an optical immersion probe, allow getting an overview of the biochemical state of the process in time. Thus, by applying the appropriate chemometric methods, it is possible to obtain biological information from the spectra, including the prediction of metabolite concentrations throughout the cultures. The research work presented here proposes to highlight the necessity to optimize spectral acquisition and statistical preprocessing of the data provided by different cell cultures. Then, robust regression models are developed, taking into account different sources of variability, such as inter-cultures variations, process parameters changes, optical probe repositioning and cell line variations. Finally, these spectra are used to determine the antibody concentration during the culture and to develop new tools for statistical process control of the batches. In this way, any abnormal behavior such as contamination can be detected
Chekireb, Djamel. "Culture de S. Cerevisiae à fortes concentrations cellulaires." Compiègne, 1986. http://www.theses.fr/1986COMPI048.
Trémouillaux-Guiller, Jocelyne. "Etude comparative des méthodologies de sélection de cultures cellulaires végétales à haute capacité d'accumulation : application à des souches et lignées clonales biosynthétisant des alcaloides dihydrofuoquinoleiques." Tours, 1988. http://www.theses.fr/1988TOUR3804.
Li, Mengyao. "Approche méthodologique innovante pour le suivi en ligne de procédés de production d’anticorps par cellules animales : apport des techniques spectroscopiques in situ à la stratégie PAT." Electronic Thesis or Diss., Université de Lorraine, 2018. http://www.theses.fr/2018LORR0151.
Bioprocesses of mammalian cell culture have become essential for the production of therapeutic recombinant proteins, such as monoclonal antibodies (mAb). However, the physiological state of the cells and the quality of the mAb produced, in particular their glycosylation, may vary during the process, and may lead to the alteration of the safety and efficacy of the final product. Consequently, the Process Analytical Technology (PAT) initiative has encouraged the development of online monitoring techniques, with the aim to better control the process and ensure the quality of the final product. In this context, this thesis proposes innovative approaches for online monitoring of CHO (Chinese Hamster Ovary) cells bioreactor cultures, by using three types of in situ spectroscopic measurements (dielectric, Raman, near infrared (NIR)). The first chapter presents a novel approach to predict in real-time one of the major cell physiological state parameters, the specific growth rate (µ). Based on online permittivity measured by in situ dielectric spectroscopy, the cell concentration was estimated and µ was calculated in real-time, making possible to detect the critical moment when µ begins to decrease significantly. Compared to an offline approach, this online approach allowed to maintain the cells in a stable physiological state, ensuring the glycosylation of the mAb produced in feed-harvest cultures. The second chapter shows the use of in situ NIR and Raman spectroscopies combined with chemometric methods. For the first time, the performances of these two spectroscopies were compared in parallel in the same cultures. Online models were developed to predict in real-time the concentration of different parameters (viable cells, glucose, lactate, glutamine, ammonium ions and antibodies). The evaluation of these models by the multivariate Figures of Merit (FOM) revealed some of the advantages of Raman spectroscopy. The combination of the two spectroscopies by various data fusion strategies has also been evaluated. In the third chapter, the interest of Raman spectroscopy for the online monitoring of both the quantity and the glycosylation of the mAb was demonstrated. Models were developed for online prediction of both macroheterogeneity (glycosylation site occupancy) and microheterogeneity (glycan structures) of mAb glycosylation in batch and feed-harvest cultures. The last chapter used models previously developed for NIR and dielectric spectroscopies, to integrate into a “soft sensor” by combining with cell metabolic and mass balance equations. This “soft sensor”, implemented in a fed-batch cell culture for the automatic control of the feed rate, leads to an increased mAb productivity and better mAb glycosylation
Denner, Aurélia. "Application de la démarche PAT pour le suivi en ligne et le contrôle de paramètres fonctionnels de cellules animales cultivées en bioréacteur." Electronic Thesis or Diss., Université de Lorraine, 2022. http://www.theses.fr/2022LORR0260.
Bioprocesses implementing animal cell culture have become essential for obtaining therapeutic bioproducts. However, it remains essential to better predict, optimize and control them. This requires, in particular, the study of new experimental and methodological tools allowing in particular the on-line analysis of related parameters, either to the cells or to the recombinant proteins produced. Thus, to meet this challenge, the PAT (Process Analytical Technology) directive recommends developing methodologies for on-line monitoring of processes for their conduct in real time, in particular by means of spectroscopic methods. In this context, this subject proposes to take up the challenge of identifying, by in-line spectroscopies, functional parameters of CHO (Chinese Ovarian Cell) animal cells producing monoclonal antibodies (mAbs). In the first chapter, the study of culture media was discussed in order to define an optimal environment for the production of mAbs by animal cells. The development of efficient prediction models is discussed from two angles: the second chapter deals with the analysis of the preprocessing of spectral data obtained in real time from spectroscopic sensors (Raman spectroscopy), then the study of the regressions used is discussed in chapter III. From the latter, the prediction of more complex parameters, such as the specific growth rate µ or the metabolic yield YX/S could be calculated accurately. The results obtained from these two chapters have been combined in chapter IV. The different developed models are compared there with the help of factors of merit reflecting their reliability. Finally, with the help of the optimal model, it was possible to control the feeding of bioreactors in real time. The last chapter presents the results obtained from these experiments in semi-continuous mode and shows a real progress for the closed-loop control of bioprocesses in order to ensure the maintenance of the optimal characteristics for the process performances
Madiedo-Podvršan, Sabrina. "Development of a lung-liver in vitro coculture model for the risk assessment of inhaled xenobiotics." Electronic Thesis or Diss., Compiègne, 2022. http://www.theses.fr/2022COMP2703.
Urbanization and globalization are prevailing social phenomena that multiply and complexify the sources of modern pollution. Amongst others, air pollution has been recognized as an omnipresent life-threatening hazard, comprising a wide range of toxic airborne xenobiotics that expose man to acute and chronic threats. The defense mechanisms involved in hazardous exposure responses are complex and comprise local and systemic biological pathways. Due to this complexity, animal models are considered prime study models. However, in light of animal experimentation reduction (3Rs), we developed and investigated an alternative in vitro method to study systemic-like responses to inhalationlike exposures. In this context, a coculture platform was established to emulate interorgan crosstalks between the pulmonary barrier, which constitutes the route of entry of inhaled compounds, and the liver, which plays a major role in xenobiotic metabolism. Both compartments respectively comprised a Calu-3 insert and a HepG2/C3A biochip which were jointly cultured in a dynamically-stimulated environment for 72 hours. The present model was characterized using acetaminophen (APAP), a well-documented hepatotoxicant, to visibly assess the passage and circulation of a xenobiotic through the device. Two kinds of models were developed: (1) the developmental model allowed for the technical setup of the coculture, and (2) the physiological-like model better approximates a vivo environment. Based on viability, and functionality parameters the developmental model showed that the Calu-3 bronchial barrier and the HepG2/C3A biochip can successfully be maintained viable and function in a dynamic coculture setting for 3 days. In a stress-induced environment, present results reported that the coculture model emulated active and functional in vitro crosstalk that seemingly was responsive to high (1.5 and 3 mM) and low (12 and 24 μM) xenobiotic exposure doses. Lung/liver crosstalk induced modulation of stress response dynamics, delaying cytotoxicity, proving that APAP fate, biological behaviors and cellular stress responses were modulated in a broader systemic-like environment
Abeille, Fabien. "Automatisation et intégration d'un réacteur de culture cellulaire pour un fonctionnement en continu." Thesis, Grenoble, 2014. http://www.theses.fr/2014GRENS036/document.
Over the past six decades, cell culture has become a common practice. It is a major tool in biological research for the understanding of life science, such as the study of disease and the discovery of new drugs. It plays an important role in many industries since it is involved in the production of many food, cosmetic, and pharmaceutical products.However, Research and the industry are now facing some limits and are expressing needs to be addressed. They are both associated with high costs due to a large consumption of resources (cells, reagents, qualified operators). More specifically, cell culture in research is characterized by low throughput of experiments, important variability and risk of contamination due to the recurrent manual operations performed by operators. Additionally, experiments are performed in static conditions and on models (2D cultures, animals…) which poorly resemble the human physiology. Industrial cell culture needs miniaturized systems that mimic the large scale bioreactors and offer higher screening possibilities.Microfluidic cell culture systems represent a promising tool to address the aforementioned issues and needs. The change of physical behaviors at the small-scale in microfluidic devices allow controlling temporally and spatially the cell microenvironment, unattainable with conventional cell culture methods. The level of automation and integration allows the substantial increase of the number of experience per system and considerable reduction of resource consumption. Thus, many small cellular 3D architectures grown under dynamic conditions and in high-throughput have been performed and have demonstrated their ability to quickly re-create more physiological environments. Regarding the industrial culture, miniaturized cultures have already shown their ability to reproduce the characteristics of the culture observed in macrobioreactors with higher screening capabilities.In this framework, a benchtop microfluidic bioreactor, complying with the standard microfluidic platform and format used in the host laboratory, has been successfully fabricated to perform continuous cell cultures. Integrated solutions were developed to provide continuously the adequate conditions for cell proliferation (perfusion, thermal regulation…). Integrated cell harvest was also performed with the final goal to achieve long-term cell culture in the bioreactor.The fabricated system proved to guarantee sterile conditions for cell cultures on a regular lab bench. Moreover, these cultures were achieved autonomously without requiring a cumbersome incubator. In these conditions, the bioreactor demonstrated the possibility to perform continuous cell cultures of various cell types during several days: insects cells were cultured during 5 days and mammalian cells during 3 days. Regarding the mammalian cell cultures performed, a breakthrough has been achieved compared to the cultures performed in microfluidic systems since microcarriers (diam.:175 µm) were used as growth support.Although microcarrier cell culture is routinely performed in the industry, no autonomous microfluidic culture system has addressed this type of culture yet. Such a miniaturization is a major step forward for bioprocess applications where the need to develop scale-down bioreactors that mimic large scale operation has been clearly identified to shorten and reduce the costs associated to bioproduct development
Leroux, Mélanie. "Production de glycosaminoglycanes par voie microbiologique et enzymatique." Thesis, Université Grenoble Alpes (ComUE), 2019. http://www.theses.fr/2019GREAV024.
Glycosaminoglycans (GAGs) are long linear polysaccharide chains, found in all animals. Some pathogenic bacteria also synthesize polysaccharides identical or similar to human GAGs. This thesis deals with chondroitin sulfate and heparosan syntheses, members of the GAGs family. There is a growing interest in these two GAGs in the pharmaceutical industry due to numerous potential applications they offer. Chondroitin sulfate is currently extracted from animal tissues which can lead to sanitary problems such as viral or prion contaminations. On the other hand, a production process still needs to be developed for heparosan. Therefore, it is necessary to develop new methods for the production of these two polymers. Enzymatic synthesis, which is a promising alternative for the production of chondroitin sulfate and heparosan, was the subject of this thesis
Dhainaut, Frédéric. "Culture de cellules de lignées épithéliales de foie de rat : influence des milieux contenant ou non du serum ou totalement aprotéique sur la croissance cellulaire sur supports stationnaires ou sur microsupports sphériques en suspension dans un biogénérateur." Dijon, 1987. http://www.theses.fr/1987DIJOS006.
Books on the topic "Cultures cellulaires – Biotechnologie":
S, Ozturk Sadettin, and Hu Wei-Shou 1951-, eds. Cell culture technology for pharmaceutical and cell-based therapies. New York: Taylor & Francis, 2006.
Butler, Mike. Animal Cell Culture and Technology : The Basics (Basics (Oxford, England).). 2nd ed. BIOS Scientific Publ, 2004.
Hu, Wei-Shou, and Sadettin S. Ozturk. Cell Culture Technology for Pharmaceutical and Cell-Based Therapies. Taylor & Francis Group, 2008.
Hu, Wei-Shou, and Sadettin Ozturk. Cell Culture Technology for Pharmaceutical and Cell-Based Therapies. Taylor & Francis Group, 2005.
Hu, Wei-Shou, and Sadettin Ozturk. Cell Culture Technology for Pharmaceutical and Cell-Based Therapies. Taylor & Francis Group, 2005.
Conference papers on the topic "Cultures cellulaires – Biotechnologie":
Le Choismier, H. "Un transporteur d’oxygène universel d’origine marine au service de la santé." In 66ème Congrès de la SFCO. Les Ulis, France: EDP Sciences, 2020. http://dx.doi.org/10.1051/sfco/20206601009.