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Academic literature on the topic 'Culture filtrate'

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Published: 4 June 2021
Last updated: 25 January 2023

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Journal articles on the topic "Culture filtrate"

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Chiu, Yi-Shu, Pi-Yu Chen, Tung Kuan, Po-Chuan Wang, Ying-Ju Chen, Yu-Liang Yang, and Hsin-Hung Yeh. "A Polysaccharide Derived from a Trichosporon sp. Culture Strongly Primes Plant Resistance to Viruses." Molecular Plant-Microbe Interactions® 31, no. 12 (December 2018): 1257–70. http://dx.doi.org/10.1094/mpmi-01-18-0012-r.

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Plant viruses cause devastating diseases in plants, yet no effective viricide is available for agricultural application. We screened cultured filtrates derived from various soil microorganisms cultured in vegetable broth that enhanced plant viral resistance. A cultured filtrate, designated F8 culture filtrate, derived from a fungus belonging to the genus Trichosporon, induced strong resistance to various viruses on different plants. Our inoculation assay found the infection rate of Tobacco mosaic virus (TMV)-inoculated Nicotiana benthamiana with F8 culture filtrate pretreatment may decrease to 0%, whereas salicylic acid (SA)-pretreated N. benthamiana attenuated TMV-caused symptoms but remained 100% infected. Tracking Tobacco mosaic virus tagged with green fluorescence protein in plants revealed pretreatment with F8 culture filtrate affected the initial establishment of the virus infection. From F8 culture filtrate, we identified a previously unknown polysaccharide composed of D-mannose, D-galactose, and D-glucose in the ratio 1.0:1.2:10.0 with a α-D-1,4-glucan linkage to be responsible for the induction of plant resistance against viruses through priming of SA-governed immune-responsive genes. Notably, F8 culture filtrate only triggered local defense but was much more effective than conventional SA-mediated systematic acquired resistance. Our finding revealed that microbial cultured metabolites provided a rich source for identification of potent elicitors in plant defense.
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Mehta, Y. R., and R. L. Brogin. "Phytotoxicity of a Culture Filtrate Produced by Stemphylium solani of Cotton." Plant Disease 84, no. 8 (August 2000): 838–42. http://dx.doi.org/10.1094/pdis.2000.84.8.838.

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Stemphylium solani, which causes a new leaf blight of cotton, was suspected of producing a phytotoxin. Studies were conducted to examine the relationship between the reaction of different cotton cultivars and of some unrelated host species to the pathogen and its toxin-containing culture filtrates. Seven single spore isolates of S. solani from cotton and their toxin-containing culture filtrates were used for leaf and root bioassays. An isolate of S. solani from tomato was also used for comparison. The phytotoxic effect was isolate dependent. Culture filtrates of five isolates killed 40 to 60% of the cotton seedlings when incubated for 4 days at 10-1 dilution. At 10-2 dilution, the culture filtrates of most of the isolates affected the development of the root system but failed to kill any seedling. The phytotoxic effect of the culture filtrate was not degraded by autoclaving. A high correlation coefficient between the percentage of the leaf area infected (LAI) by S. solani and the percentage of the necrotic leaf area (LAN) by the culture filtrate was observed when one of the aggressive isolates and its culture filtrate were tested against adult plants of 38 cotton cultivars (r = 0.86). Cultivars CNPA T-1180-23, CNPA-PRECOCE 2, PR 94-215, and PR 94-82 demonstrated resistance to the pathogen as well as insensitivity or moderate sensitivity to its toxin. Cultivars showing intermediate reaction to the pathogen also showed intermediate reaction to its culture filtrate. Similarly, the highly susceptible cultivars Paraná 3, PR 93-129, and PR 94-216 also were highly sensitive to the culture filtrate. Of the 18 plant species belonging to 18 genera, eight were susceptible to the pathogen. With two exceptions, susceptible hosts were also sensitive to the culture filtrate, whereas nonsusceptible hosts were insensitive. A component of the culture filtrate was regarded as a pathogenicity factor.
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Han, Richou, and Ralf-Udo Ehlers. "Trans-specific nematicidal activity of Photorhabdus luminescens." Nematology 1, no. 7 (1999): 687–93. http://dx.doi.org/10.1163/156854199508711.

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Abstract Mixed culture filtrates of Photorhabdus luminescens isolated from Heterorhabditis bacteriophora H06 and from H. indica LN2 had a toxic effect on axenic H. bacteriophora H06 dauer juveniles. Single culture filtrates had no effect. When one filtrate originated from a secondary phase culture, the toxic effect was lost. Heat treatment of one of the filtrates to 80°C destroyed the effect. The toxin is probably synthesized de novo after mixing the culture supernatants of these two P. luminescens symbionts. Dilution of the filtrate reduced the effect, and it was lost when P. luminescens was cultured for more than two days. Steinernema carpocapsae A24 was not affected by the mixture, and was affected only by the filtrate of primary phase P. luminescens H06. The toxic effect was recorded also when axenic dauer juveniles of H. bacteriophora were inoculated into a mixed bacterial culture of H06 and LN2. Inoculating monoxenic dauer juveniles of H. bacteriophora H06 into P. luminescens LN2 or into mixtures containing LN2 bacteria resulted in significant dauer juvenile mortality. These manifestations of the interaction of bacteria to produce toxic effects on the non-symbiotic nematode (trans-specific activity) may have an impact on competitive interactions when one insect host is infected by different nematode species. Eine transspezifische nematizide Aktivitat von Photorhabdus luminescens - Eine Mischung der Kulturfiltrate des Bakteriums Photorhabdus luminescens, isoliert aus Heterorhabditis bacteriophora H06 mit dem Filtrat des Symbionten aus H. indica LN2 wirkt toxisch auf axenische Dauerlarven von H. bacteriophora H06. Die einzelnen Kulturfiltrate zeigten keine Wirkung. Sofern ein Filtrat einer Sekundarformkultur entnommen wurde, konnte kein Effekt erzielt werden. Eine Hitzebehandlung bei 80°C zerstorte die Wirkung. Das Toxin wird wahrscheinlich de novo synthetisiert in dem Moment, in dem die Kulturfiltrate dieser P. luminescens Symbionten gemischt werden. Eine Verdunnung der Filtrate reduzierte die Wirkung. Sie ging ganz verloren, wenn P. luminescens langer als zwei Tage kultiviert wurde. Die Mischung hatte keine Wirkung auf axenische Steinernema carpocapsae. Dieser Nematode wurde nur durch Filtrate der Primarform von P. luminescens H06 abgetotet. Die toxischen Effekte wurden auch festgestellt, wenn axenische Dauerlarven von H. bacteriophora in Gemische von H06 und LN2 Bakterienkulturen inokuliert wurde. Bei Inokulation von monoxenischen Dauerlarven von H. bacteriophora H06 in P. luminescens LN2 oder in Gemische, die LN2 enthielten, wurde ebenfalls eine gesteigerte Mortalitat festgestellt. Dieses Deutlichwerden von Wechselbeziehungen zwischen den Bakterien bei Erzeugung toxischer Effekte auf den nicht-symbiontischen Nematoden (trans-spezifische Aktivitat) konnte eine Auswirkung auf die Konkurrenz bei Koinfektion eines Wirtsinsekts mit zwei Nematodenarten haben.
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Proletova, N. V. "DEPENDENCE OF PHYTOTOXICITY OF CULTURAL FILTRATES OF THE FLAX ANTHRACNOSE PATHOGEN COLLETOTRICHUM LINI MANNS ET BOLLEY STRAINS ON THE AMINO ACID COMPOSITION." Bulletin of NSAU (Novosibirsk State Agrarian University), no. 3 (October 24, 2020): 66–75. http://dx.doi.org/10.31677/2072-6724-2020-56-3-66-75.

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The aim of this work is to determine the amino acid composition of the cultural filtrates of the flax anthracnose fungus Colletotrichum lini Manns et Bolley strains to adjust the concentration of the selective agent in the nutrient medium when creating new flax genotypes resistant to anthracnose in vitro. It was found that the cultural filtrates of strains 527 and 608 contain such amino acids as alanine, glycine, asparagine, cysteine, threonine, aspartic acid, glutamic acid, as well as arginine in the highly virulent strain 527. The traces of tyrosine and lysine in the weakly virulent strain 608 were also found. On the day of cultivation, the supply of nutrients in the cultivation medium was apparently depleted, and the fungus began to use the products of its vital activity for life support. In the culture filtrate of the highly virulent strain 527, the concentration of all certain amino acids was significantly higher than in the culture filtrate of the weakly virulent strain 608. It was shown that the 23-day culture filtrate of the highly virulent strain 527 had the highest toxicity which is lower than in all genotypes taken in the study. The toxicity of the culture filtrate depends on the virulence of the anthracnose pathogen strain. The culture filtrate of a highly virulent strain is more toxic than a weakly virulent one. The presence of cysteine in the culture filtrates of the strains increases the possibility of inhibiting the growth and development of flax cells in in vitro culture. When using the culture filtrate of anthracnose pathogen strains containing asparagine, glutamine, serine, glycine, aspartic and glutamic acids, it is possible to induce the growth and development of flax cells in vitro. As the fungal mycelium grew in the culture filtrates, the concentrations of alanine, asparagine, glycine, aspartic and glutamic acids decreased. Due to the high concentration of cysteine and tyrosine, the culture filtrates of strains 419 and 639 were toxic during the entire study period (up to 42 days).
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HARNI, RITA, SUPRAMANA SUPRAMANA, MEITY S. SINAGA, GIYANTO GIYANTO, and SUPRIADI SUPRIADI. "PENGARUH FILTRAT BAKTERI ENDOFIT TERHADAP MORTALITAS, PENETASAN TELUR DAN POPULASI NEMATODA PELUKA AKAR Pratylenchus brachyurus PADA NILAM." Jurnal Penelitian Tanaman Industri 16, no. 1 (June 19, 2020): 43. http://dx.doi.org/10.21082/jlittri.v16n1.2010.43-47.

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<p>ABSTRAK</p><p>Pratylenchus brachyurus merupakan salah satu patogen utama padatanaman nilam di Indonesia. Pengendalian yang banyak dilakukan petanisaat ini adalah menggunakan pestisida sintetik. Penggunaan pestisidasintetik yang terus menerus merupakan ancaman terhadap lingkungan, dankesehatan manusia. Bakteri endofit mungkin dapat dimanfaatkan sebagaisalah satu teknik pengendalian nematoda yang ramah lingkungan karenabakteri endofit dapat menghasilkan racun yang toksik terhadap nematoda.Tujuan penelitian adalah melihat pengaruh kultur filtrat bakteri endofitterhadap mortalitas nematoda, penetasan telur dan perkembangannematoda di dalam akar nilam. Penelitian dilakukan di Laboratorium danRumah kaca Hama dan Penyakit Balai Penelitian Tanaman Obat danAromatik Bogor, dari bulan Januari sampai April 2008 menggunakanrancangan acak lengkap (RAL). Filtrat bakteri dibuat dengan caramenumbuhkan bakteri endofit pada media TSB selama 48 jam, kemudiandisentrifugasi dengan kecepatan 7.000 rpm selama 15 menit. Filtratdisaring dengan milipore berdiameter 0,22 µm, selanjutnya filtrat diujipada nematoda in vitro dan rumah kaca. Hasil penelitian menunjukkanbahwa filtrat dapat membunuh nematoda dalam waktu 24 jam dengannilai LC 50 sebesar 7,709%. Bakteri endofit isolat TT2 dan EH11memperlihatkan daya bunuh paling tinggi yaitu 91-100%. Di samping itufiltrat bakteri endofit juga dapat menekan penetasan telur nematoda 48,5-74,6% dibanding dengan kontrol. Namun hanya filtrat bakteri endofitisolat EH11 yang nyata dapat menekan populasi nematoda di dalam akarnilam dengan tingkat penekanan sebesar 81,3%.</p><p>Kata kunci : Pratylenchus brachyurus, bakteri endofit, kultur filtrat,Pogostemon cablin</p><p>ABSTRACT</p><p>Effect of culture filtrates endophytic bacteria on themortality, hatching eggs and population of root lesionnematodes Pratylenchus brachyurus on patchouli</p><p>Root lesion nematode (Pratylenchus brachyurus) is an importantpathogen of patchouli in Indonesia and causes significant losses. Controlsystem that are done today is using synthetic pesticides. The use ofsynthetic pesticides is a continuing threat to the environment and humanhealth. However, endophytic bacterial culture filtrates may be used as oneof the nematode control that is environmentally friendly. Effect of culturefiltrates endophytic bacteria on the mortality, hatching eggs and populationroot lesion nematodes Pratylenchus brachyurus on patchouli has beendone in vitro and greenhouse. The results showed that the culture filtrate ofendophytic bacteria produced metabolite toxic to nematodes and wereable to kill P. brachyurus 100% within 24 hours with LC 50 7.709%. TT2and EH11 isolates showed high killing power of 91-100%. The culturefiltrates also inhibited hatching of P. brachyurus eggs compared withcontrols. Not all culture filtrates can suppress the nematode population inthe roots of patchouli. EH11 isolates filtrate really pressing nematodepopulations compared to other isolates.</p><p>Key words: Pratylenchus brachyurus, culture filtrate, endophyticbacteria, Pogostemon cablin</p>
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Shivangi S. Kansara, M. Sruthy. "Effect of Culture Filtrates of Dominant Seed Mycoflora of Chilli on Seed Germination and Seedling Vigour." International Journal of Current Microbiology and Applied Sciences 11, no. 3 (March 10, 2022): 178–87. http://dx.doi.org/10.20546/ijcmas.2022.1103.021.

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Effect of culture filtrates of most common and dominant fungi viz., Aspergillus niger, Colletotrichum sp. and Fusarium sp. of chilli seed variety GVC 101 and GVC 111 was studied on seed germination, seedling length, fresh weight, dry weight and vigour index.In GVC 101 variety, the seeds treated with culture filtrate of A. niger showed maximum decrease in seed germination (43.35%) followed by Colletotrichumsp. (20.23%) and Fusarium sp. (9.83%). Average seedling length was also decreased maximum in seeds treated with culture filtrate of A. niger (43.70%) followed by Colletotrichum sp. (40.22%) and Fusarium sp. (23.91%). Seeds treated with culture filtrate of A. niger showed maximum reduction of vigour index by 68.09 per cent followed by Colletotrichum sp. (52.31%) and Fusarium sp. (31.34%). In GVC 111 variety, the seeds treated with culture filtrate of A. niger showed maximum decrease in seed germination (35.12%) followed by Colletotrichum sp. (17.89%) and Fusarium sp. (15.12%). Average seedling length was also decreased maximum in seeds treated with culture filtrate of A. niger (61.14%) followed by Colletotrichum sp. (36.64%) and Fusarium sp. (25.54%). Seeds treated with culture filtrate of A. niger showed maximum reduction of vigour index by 74.73 per cent followed by Colletotrichumsp. (47.91%) and Fusarium sp. (36.73%).
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Aminin, Agustina L. N., Nur Cahyanti, Alfina Sari, Nies Suci Mulyani, and Bambang Cahyono. "Antioxidant and Antimicrobial Screening of Endophytic Fungi Culture Filtrate from Purwoceng (Pimpinella alpina Molk) Leaf." Jurnal Kimia Sains dan Aplikasi 23, no. 9 (September 7, 2020): 319–24. http://dx.doi.org/10.14710/jksa.23.9.319-324.

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This is a preliminary study to determine the bioactivity potential of purwoceng leaf endophytic fungal metabolites. Endophytic fungi were isolated from purwoceng leaf and their secondary metabolite from culture filtrate were subjected to identify the antimicrobial, antioxidant, and phytochemical screening. The antioxidant activity was screened by scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH). The antimicrobial activity was screened using a good agar method toward Salmonella typhi, Escherichia coli, Bacillus subtilis, Staphylococcus aureus, dan Candida albicans. This study obtained five distinctive endophytic fungi isolates named A, B, C, D, and E. The endophytic fungal culture filtrate of C has the most extensive antimicrobial activity with phytochemical screening showing alkaloids, saponins, and terpenoids. The antioxidant potential of all culture filtrates seemed low because the DPPH amount was interfered with by pigment compounds. Culture filtrate of fungi A showed the highest antioxidant activity and contained phenolic and alkaloid compounds.
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Park, Ja-On, Krishnapillai Sivasithamparam, Emile Ghisalberti, Jaih Hargreaves, Walter Gams, and Anthony L. J. Cole. "Cuticular disruption and mortality of Caenorhabditis elegans exposed to culture filtrate of Byssochlamys nivea Westling." Nematology 3, no. 4 (2001): 355–63. http://dx.doi.org/10.1163/156854101317020277.

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AbstractA strain of a Byssochlamys nivea, isolated from saline mud in Western Australia as a part of statewide survey of soil fungi for nematophagous activity, was evaluated for its effect on nematodes. Culture filtrate of the fungus grown on potato dextrose broth for 7 days caused structural changes in the cuticle, aggregation of individuals, and mortality of Caenorhabditis elegans. In addition, the culture filtrate completely inhibited hatching of C. elegans eggs. Exudates from agar colonies also caused cuticular disruption and mortality of C. elegans. The cuticular disruption observed, not reported in nematodes before, was initiated in the labial region and spread towards the posterior region of the nematode within 10 min of application. This reaction occurred only in live nematodes. Cuticular disruption and mortality caused by the culture filtrate varied according to growth conditions. The active compound(s) in the culture filtrate were thermostable (100°C for 1 h); however freezing the culture filtrate (-20°C for 2 days) eliminated the activities, as did dialysis (<14 000 molecular weight). Cuticular disruption and mortality were also observed when the nematode was exposed to culture filtrates of two other strains of B. nivea supplied by CBS, The Netherlands. The culture filtrate also inhibited in vitro growth of the plant-pathogenic fungi Fusarium oxysporum, Gaeumannomyces graminis var. tritici, Phytophthora cinnamomi, Pythium irregulare and Rhizoctonia solani.
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Adekunle, O. K., and O. A. Akinsanmi. "Bioactivity of Fusarium oxysporum f. sp. glycines and Sclerotium rolfsii filtrates on egg hatching, survival and infectivity of juveniles of Meloidogyne incognita race 2." Australian Journal of Experimental Agriculture 45, no. 1 (2005): 99. http://dx.doi.org/10.1071/ea02129.

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The effects of culture filtrates of Fusarium oxysporum and Sclerotium rolfsii on egg hatching and juvenile survival of Meloidogyne incognita in vitro and impact of these filtrates on infectivity of M. incognita were investigated on soybean seedlings. Five- and 10-day-old filtrates of F. oxysporum caused 65 and 54% egg-hatching inhibition, while that of S. rolfsii caused 61 and 49% inhibition, respectively. Juveniles of M. incognita died within 6 days when incubated in 5-day-old filtrate of F. oxysporum, while the similar filtrate of S. rolfsii caused 100% juvenile mortality on the fifth day. Filtrates reduced root galling, egg population, number of adult females in soybean plants at harvest and also soil population. Culture filtrates could be used as source of biological nematicides.
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Parveen, Shazia, Abdul Hamid Wani, and Mohd Yaqub Bhat. "Effect of culture filtrates of pathogenic and antagonistic fungi on seed germination of some economically important vegetables." Brazilian Journal of Biological Sciences 6, no. 12 (2019): 133–39. http://dx.doi.org/10.21472/bjbs.061212.

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The subject of present study was to check whether the pathogenic fungi that were associated with different rot diseases of fruits and vegetables and the antagonistic fungal species produce extracellular growth regulating substances. For this present study healthy seeds of four economically important crop plants, viz. Solanum lycopersicum, Brassica rapa, Raphanus sativus and Trigonella melongena were selected. The results showed that all the pathogenic fungi except Fusarium solani decrease the germination percentage of the all seeds. Solanum lycopersicum seed germination was completely inhibited by the culture filtrate of Trichothecium roseum and Alternaria alternata. Likewise, the culture filtrate of Penicillium expansum caused complete inhibition of the germination of Brassica rapa seeds. The culture filtrate of Fusarium solani was found to increase the germination percentage of all the seeds tested during the present study. Amongst the three Trichoderma spp., T. asperellum and T. harzianum culture filtrate effectively increases the seed germination percentage of all the seeds tested while the culture filtrate of T. viride have negative effect on the germination percentage of Solanum lycopersicum, Brassica rapa, and Raphanus sativus seeds. This stimulatory or inhibitory effect of the culture filtrates can be attributed to the presence of certain metabolites/substances that the test fungi have released in the medium. To identify the substances present and the nature of these substances further studies will be carried out.
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Dissertations / Theses on the topic "Culture filtrate"

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Rennie, Bryan D. "Detection and identification of antigens from Mycobacterium bovis culture filtrate with immune sera from Mycobacterium bovis sensitized or infected cattle." Thesis, University of Ottawa (Canada), 2009. http://hdl.handle.net/10393/28138.

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Bovine tuberculosis, caused by Mycobacterium bovis, infects approximately 50 million cattle worldwide and is diagnosed by the tuberculin skin test (TST). The purpose of this thesis was to characterise the culture filtrate proteins (CFP) of M. bovis PPD tuberculin and to compare the antibody response of M. bovis infected versus M. bovis sensitized cattle. Sterile filtered PPD tuberculin (SF-PPD) resolved into approximately 200 discrete spots using two-dimensional PAGE. While 2D Western blot analysis of both M. bovis sensitized and M. bovis infected cattle sera demonstrated an antibody boost following comparative intradermal TSTs, M. bovis sensitized cattle responded with greater intensity to additional SF-PPD antigens as compared to M. bovis infected cattle at seven weeks post infection/sensitization. In conclusion M. bovis sensitized cattle generated a more intense antibody response and recognized additional SF-PPD antigens as compared to M. bovis infected cattle within the first two months post infection/sensitization.
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Kanu, S., and Dakora FD. "Thin-layer chromatographic analysis of lumichrome, riboflavin and indole acetic acid in cell-free culture filtrate ofPsoralea nodule bacteria grown at different pH, salinity and temperature regimes." Symbiosis, 2009. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1001481.

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Abstract Using thin-layer chromatography, 16 bacterial isolates from root nodules of 8 different Psoralea species were quantitatively assessed for their exudation of the metabolites lumichrome, riboflavin and IAA in response to pH, salinity and temperature. Our data showed that the bacterial strains tested differed in their levels of secretion of the three metabolites. For example, strain AS2 produced significantly greater amounts of lumichrome at both pH 5.1 and 8.1, while strains RTl and PI produced more lumichrome per cell at only pH 8.1. Strains API and RP2 also produced more riboflavin at pH 5.1 than at pH 8.1; conversely strain RTl secreted more riboflavin at pH 8.1 than at pH 5.1. Two P. repens strains (RPI and RP2) isolated from very saline environments close to the Indian Ocean produced significant levels of lumichrome and riboflavin at both low and high salinity treatments. However, strains ACI and Ll (from P. aculeata and P. laxa) even produced greater amounts of lumichrome and riboflavin at higher salinity (i.e. 34.2 mM NaCl) and probably originated from naturally saline soils. In this study, high acidity and high temperature induced the synthesis and release of high levels of IAA by bacterial cells. In contrast, there was greater strain secretion of lumichrome at lower temperature (10°C) than at high temperature (30°C). The variations in the secretion of lumichrome, riboflavin and IAA by bacterial strains exposed to different pH, salinity and temperature regimes suggest that genes encoding these metabolites are regulated differently by the imposed environmental factors. The data from this study also suggest that natural changes of pH, salinity and/or temperature in plant rhizospheres could potentially elevate the concentrations of lumichrome, riboflavin and IAA in soils. An accumulation of these molecules in the rhizosphere would have consequences for ecosystem functioning as both lumichrome and riboflavin have been reported to act as developmental signals that affect species in all three plant, animal, and microbial kingdoms.
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Kanu, S., and FD Dakora. "Thin-layer chromatographic analysis of lumichrome, riboflavin and indole acetic acid in cell-free culture filtrate ofPsoralea nodule bacteria grown at different pH, salinity and temperature regimes." Balaban, 2009. http://encore.tut.ac.za/iii/cpro/DigitalItemViewPage.external?sp=1001676.

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Abstract Using thin-layer chromatography, 16 bacterial isolates from root nodules of 8 different Psoralea species were quantitatively assessed for their exudation of the metabolites lumichrome, riboflavin and IAA in response to pH, salinity and temperature. Our data showed that the bacterial strains tested differed in their levels of secretion of the three metabolites. For example, strain AS2 produced significantly greater amounts of lumichrome at both pH 5.1 and 8.1, while strains RTl and PI produced more lumichrome per cell at only pH 8.1. Strains API and RP2 also produced more riboflavin at pH 5.1 than at pH 8.1; conversely strain RTl secreted more riboflavin at pH 8.1 than at pH 5.1. Two P. repens strains (RPI and RP2) isolated from very saline environments close to the Indian Ocean produced significant levels of lumichrome and riboflavin at both low and high salinity treatments. However, strains ACI and Ll (from P. aculeata and P. laxa) even produced greater amounts of lumichrome and riboflavin at higher salinity (i.e. 34.2 mM NaCl) and probably originated from naturally saline soils. In this study, high acidity and high temperature induced the synthesis and release of high levels of IAA by bacterial cells. In contrast, there was greater strain secretion of lumichrome at lower temperature (10°C) than at high temperature (30°C). The variations in the secretion of lumichrome, riboflavin and IAA by bacterial strains exposed to different pH, salinity and temperature regimes suggest that genes encoding these metabolites are regulated differently by the imposed environmental factors. The data from this study also suggest that natural changes of pH, salinity and/or temperature in plant rhizospheres could potentially elevate the concentrations of lumichrome, riboflavin and IAA in soils. An accumulation of these molecules in the rhizosphere would have consequences for ecosystem functioning as both lumichrome and riboflavin have been reported to act as developmental signals that affect species in all three plant, animal, and microbial kingdoms.
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Pijut, Paula M. "Effects of culture filtrates of Ceratocystis ulmi on growth and ultrastructure of in vitro cultured Ulmus Americana /." The Ohio State University, 1988. http://rave.ohiolink.edu/etdc/view?acc_num=osu148759742413622.

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Spiassi, Ariane. "Aleopatia de fungos fitopatogênicos sobre plantas invasoras das culturas de soja e milho." Universidade Estadual do Oeste do Parana, 2011. http://tede.unioeste.br:8080/tede/handle/tede/422.

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Invasive plants can cause problems to the exploitation of agriculture worldwide. The chemical control of these plants has generated several environmental problems, such as the contamination of natural resources, the compromising the food quality, the poisoning of farmers, the development of the weed s resistance, among others. An alternative to reduce the use of pesticides is the use of biological control, using pathogenic fungi that produce a variety of secondary compounds in culture medium, which exhibit phytotoxicity. Thus, the objective of this study was to determine the allelopathic activity of culture filtrate produced by pathogenic fungi that attack soybeans (Fusarium solani, Macrophomina phaseolina) and maize (Fusarium graminearum, Diplodia maydis). We evaluated the effects of fungal filtrates at concentrations of 0, 1, 5, 10, 15 and 20% on seed germination and on the developments of both radicle and hypocotyl of Canadian horseweed (Conyza canadensis (L.) Cronq.) broomstick (Bidens pilosa L.) and milkweed (Euphorbia heterophylla L.). Such effects were also tested on cultivated plants (soy and corn). The design of the experiment was completely randomized, with six treatments and four replications. The results indicate that the filtered solution of the Fusarium solani culture presented negative effect on Canadian horseweed, broomstick and milkweed without affecting the soy negatively. Diplodia maydis provided the reduction of growth of horseweed and milkweed without causing damage to the maize culture. Finally, the one of Macrophomina phaseolina decreased the growth of milkweed plantules without affecting the maize negatively, suggesting that these filtered solutions can be used for controlling invasive plants, being an ecologically friendly alternative for the reduction of the herbicides use and for protecting the environment
Plantas invasoras causam problemas à exploração da agricultura mundial, e o controle químico dessas plantas tem gerado diversos problemas ambientais, como contaminação de recursos naturais, comprometimento da qualidade de alimentos, intoxicação de agricultores, resistência de plantas invasoras, entre outros desequilíbrios. Uma das alternativas para reduzir o uso de agrotóxicos é a utilização do controle alternativo, empregando fungos fitopatogênicos que produzem uma variedade de compostos secundários em meio de cultivo, os quais exibem atividade fitotóxica. Assim, o objetivo deste trabalho foi caracterizar a atividade alelopática do filtrado de cultura produzido por fungos fitopatogênicos que atacam a soja (Fusarium solani e Macrophomina phaseolina) e o milho (Fusarium graminearum e Diplodia maydis). Foram avaliados os efeitos in vitro dos filtrados fúngicos, nas concentrações de 0, 1, 5, 10, 15 e 20%, sobre a germinação de sementes e desenvolvimento de radícula e hipocótilo de buva (Conyza canadensis (L.) Cronq.), picão-preto (Bidens pilosa L.) e amendoim bravo (Euphorbia heterophylla L.). Também foi testado este efeito sobre as plantas cultivadas (soja e milho). O delineamento experimental foi inteiramente casualizado com vinte e um tratamentos e quatro repetições. Os dados obtidos foram submetidos à análise de variância e de regressão polinomial. Os resultados indicaram que o filtrado de cultura de Fusarium solani apresentou efeitos negativos sobre buva, picão-preto e amendoim bravo sem afetar negativamente a soja. Diplodia maydis proporcionou redução de crescimento de buva e amendoim bravo sem causar prejuízo à cultura do milho. Finalmente, o filtrado de Macrophomina phaseolina diminuiu o crescimento de plântulas de amendoim bravo sem afetar negativamente o milho, sugerindo que esses filtrados possam ser utilizados para controle das plantas invasoras em questão como alternativa ecologicamente correta na redução do consumo de herbicidas e na proteção ao ambiente
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Murayama, Takako. "Suppressive effects of Aspergillus fumigatus culture filtrates on human alveolar macrophages and polymorphonuclear leukocytes." Kyoto University, 1997. http://hdl.handle.net/2433/202229.

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Becerra-Celis, Giuliana Isambert Arsène. "Proposition de stratégies de commande pour la culture de microalgue dans un photobioréacteur continu." S. l. : Ecole centrale de Paris, 2009. http://theses.abes.fr/2009ECAP0014.

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Lévesque, Vicky. "Potentiel des marais filtrants à traiter les effluents de serre issus d'une culture de tomate." Thesis, Université Laval, 2011. http://www.theses.ulaval.ca/2011/28453/28453.pdf.

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Ce projet vise à améliorer l’efficacité des marais à éliminer les éléments minéraux présents dans les effluents provenant de cultures de tomates hors sol. Selon nos résultats, les ssh végétalisés de Typha latifolia et combinés avec un apport en carbone ont rendu plus efficace le traitement des effluents de cultures en serre. De plus, parmi les trois types de marais à l’étude (ssh, ssv et sh), les ssh semblent être les mieux adaptés pour traiter des effluents très chargés en éléments minéraux. Toutefois, les émissions de GES ne sont pas à négliger dans les ssh. Afin d’accroître la performance de traitement en terme de charge polluante ionique, des marais en série devraient être utilisés. Selon l’un de nos essais, le traitement des effluents par trois ssv en série a éliminé efficacement les différents minéraux en dessous de la limite permise au Québec.
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Pelletier, François. "Mise au point d'observateurs d'état pour le suivi de cultures de cellules animales." Vandoeuvre-les-Nancy, INPL, 1995. http://www.theses.fr/1995INPL135N.

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L’objectif de ce travail est l'élaboration d'outils d'estimation d'état simples d'emploi pour l'évaluation de la composition des milieux de culture cellulaires. La première partie concerne le développement d'un nouvel estimateur appelé observateur à base de bilans de matière construit sur le principe de l'observateur asymptotique. Il peut être appliqué à n'importe quel type de culture. Il ne nécessite aucun réglage. La possibilité de prendre en compte certaines lois cinétiques connues permet de le faire fonctionner avec un nombre restreint de mesures. La seconde partie traite de la mise au point d'une nouvelle variante du filtre de Kalman, le filtre de Kalman auto-ajustable. Le réglage du filtre de Kalman est délicat et peut conduire à des problèmes de stabilité de l'erreur d'estimation et de convergence des valeurs estimées vers les valeurs réelles. Le filtre de Kalman auto-ajustable assura la stabilité des erreurs d'estimations avec un nombre réduit de réglages. Les deux nouveaux observateurs ont été appliqués à trois cultures d'hybridomes productrices d'anticorps monoclonaux. À partir de deux mesures expérimentales (glucose et lactate déshydrogénase ou ammoniaque et lactate déshydrogénase), la composition du milieu de culture est évaluée par les deux techniques. L’observateur à base de bilans de matière a donné de bons résultats et ceux obtenus avec le filtre de Kalman auto-ajustable sont acceptables tant que l'on ne sort pas du domaine de validité du modèle
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Dildey, Omari Dangelo Forlin. "Indução de resistência a antracnose do feijoeiro por frações de filtrato de cultura e extrato de micélio de Trichoderma longibrachiatum." Universidade Estadual do Oeste do Paraná, 2017. http://tede.unioeste.br/handle/tede/3197.

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This study aimed to purify by chromatography elicitors from Trichoderma longibrachiatum culture filtrate and mycelium extract and to test them in phytoalexin phaseolin inducing and resistance induced to anthracnose in common bean. The sodium phosphate buffer at 0.05 M (SPB) was used as the control treatment and the ASM (Acibenzolar-S-Metil) was used as the standard induction treatment. Ion Exchange Chromatography (IEC) and Gel Filtration Chromatography (GFC) were performed to separate fractions with eliciting power from the culture filtrate (CF) and T. longibrachiatum mycelium extract (TME). For the purification of elicitors by IEC, from GFC, it were obtained one glycidic and five glycoproteins fractions, totaling six fractions. For purification from TME, it were obtained three protein, one glycidic and two glycoproteins fractions, totaling six fractions. In both, were obtained twelve fractions from IEC. These, in turn, were purified in GFC, being obtained a total of thirty seven fractions. Among these, there were fourteen fractions of TME were classified according to their nature, being three proteins, two glycogen and nine glycoproteins. There were twenty-three fractions from TME, wich were classified according to their nature, being four proteins, nine glycogen and ten glycoproteins. Of the fractions purified in CFG from FTC and TME, eight presented phaseolin inducer potential (F17, F23, F25, F27, F31, F38 and F46). The 10 treatments consisted of the eight fractions and two controls: ASM and control (TAP). Treatments were applied in one of the primary leaves (treated leaf (TL)), and the other primary leaf was not treated (untreated leaf (UL)) to verify the systemic effect. Three leaf samples were taken for determination of enzymatic activity: before applying the fractions, after application of the fractions and after the pathogen inoculation. The defense enzyme analysis was performed for Peroxidase (POX), Polyphenoloxidase (PFO), Catalase (CAT), Phenylalanine ammonia-lyase (PAL) and β-1,3-glucanase (β-GASE). At the end, it was performed an evaluation of severity in the primary leaf of common bean, on the fifth day after inoculation. The in vivo test data were subjected to analysis of variance. The purification of samples from FTC and EXM of T. longibrachiatum, from IEC and GFC indicated fractions with the presence of eliciting molecules. The fractions F17, F23 and F25 from FTC and F27, F29, F31, F38 and F46 from EXM were able to induce phaseolin synthesis in common bean hypocotyls. The POX, PFO and β-GASE Increased when applied in the TL after application of the fractions and after inoculation of the pathogen. The fractions did not alter CAT and FAL enzymatic activity. The fractions F17, F23 and F27 reduced the anthracnose severity in the local effect.
O trabalho teve por objetivo purificar por cromatografia moléculas eliciadoras a partir de filtrado de cultura e extrato de micélio de Trichoderma longibrachiatum e testá-las na indução de fitoalexina faseolina e com potencial indutor de resistência contra antracnose no feijoeiro. O tampão fosfato de sódio 0,05 M (TAP) foi utilizado como tratamento controle e ASM (Acibenzolar-S-Metil) foi utilizado como tratamento padrão de indução. Cromatografia de troca iônica (CTI) e cromatografia de filtração em gel (CFG) foram realizadas para separar frações com poder eliciador a partir de filtrado de cultura (FTC) e extrato de micélio (EXM) de T. longibrachiatum. Para a purificação de eliciadores por CTI, a partir de FTC, foram obtidos uma fração glicídica e cinco glicoproteicas, total de seis frações. Para a purificação a partir de EXM, forma obtidos três frações proteicas, uma glicídica e duas glicoproteicas, total de seis frações, em ambos foi totalizado doze frações obtidas por CTI. Estas, por sua vez, foram purificadas em CFG, sendo obtidos um total de trinta e sete frações. Entre essas, quatorze frações para FTC, as mesmas foram classificadas de acordo com sua natureza, sendo três proteicas, duas glicídicas e nove glicoproteicas. E vinte e três frações para EXM, as mesmas foram classificadas com sua natureza, sendo quatro proteicas, nove glicídicas e dez glicoproteicas. Das frações purificadas na CFG a partir de FTC e EXM, oito apresentaram potencial indutor de faseolina (F17, F23, F25, F27, F31, F38 e F46. Os 10 tratamentos constituíram de oito frações e dois controles: ASM e controle (TAP). Foram aplicados os tratamentos em uma das folhas primária (folha tratada (FT)), sendo que outra folha primária não recebeu tratamento (folha não tratada (FNT)) para verificar o efeito sistêmico. Foram realizadas três coletas de folhas para determinação da atividade enzimática, antes da aplicação das frações, depois da aplicação das frações e coleta após a inoculação do patógeno. Foi realizada análise enzimática (peroxidase (POX), polifenoloxidase (PFO), catalase (CAT), fenilalanina amônia-liase (FAL) e β-1,3-glucanase (β-GASE)) e ao final, realizada avaliação de severidade no quinto dia após a inoculação da folha primária de feijoeiro. A purificação de amostras provenientes de FTC e EXM de T. longibrachiatum, por CTI e CFG indicaram frações com presença de moléculas eliciadoras. As frações F17, F23 e F25 de origem FTC e F27, F29, F31, F38 e F46 de origem EXM foram capazes de induzir a síntese de faseolina em hipocótilos de feijoeiro. A atividade de POX, PFO e β-GASE aumentou quando aplicado na FT após a aplicação das frações e após a inoculação do patógeno. As frações não alteraram a atividade enzimática de CAT e FAL. As frações F17, F23 e F27 reduziram a severidade da antracnose no efeito local.
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Books on the topic "Culture filtrate"

1

Murmanis, L. The action of isolated brown-rot cell-free culture filtrate, HO-Fe++ and the combination of both on wood. Madison, WI: [Forest Products Laboratory], 1988.

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Murmanis, L. The action of isolated brown-rot cell-free culture filtrate, HÓ-́Fe++ and the combination of both on wood. Madison, WI: [Forest Products Laboratory], 1988.

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Pineira-Tresmontant, Carmen. La présidentielle 2007 au filtre des médias étrangers: Actes du colloque international organisé par l'Université d'Artois, EA 4028: "Textes et cultures", Arras, du 13 au 15 mars 2008. Paris: L'Harmattan, 2008.

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Pineira-Tresmontant, Carmen. La présidentielle 2007 au filtre des médias étrangers: Actes du colloque international organisé par l'Université d'Artois, EA 4028: "Textes et cultures", Arras, du 13 au 15 mars 2008. Paris: L'Harmattan, 2008.

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La présidentielle 2007 au filtre des médias étrangers: Actes du colloque international organisé par l'Université d'Artois, EA 4028: "Textes et cultures", Arras, du 13 au 15 mars 2008. Paris: L'Harmattan, 2008.

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Carmen, Pineira-Tresmontant, and Université d'Artois. Équipe d'accueil Textes et cultures., eds. La présidentielle 2007 au filtre des médias étrangers: Actes du colloque international organisé par l'Université d'Artois, EA 4028: "Textes et cultures", Arras, du 13 au 15 mars 2008. Paris: L'Harmattan, 2008.

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Book chapters on the topic "Culture filtrate"

1

Adya, Ashok K., Anju Gautam, Lixin Zhang, and Ajit Varma. "Characterization of Piriformospora indica Culture Filtrate." In Soil Biology, 345–75. Berlin, Heidelberg: Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-33802-1_21.

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Konecna, Klara, Martin Hubalek, and Lenka Hernychova. "Analysis of Culture Filtrate Proteins of Francisella Tularensis." In BSL3 and BSL4 Agents, 107–14. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA, 2011. http://dx.doi.org/10.1002/9783527638192.ch9.

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Crinò, P., R. Penuela, L. Martino, A. Sonnino, and G. Ancora. "In Vitro Reaction of Potato Micronodes to Culture Filtrate of Phytophthora Infestans." In Phytotoxins and Plant Pathogenesis, 441–42. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73178-5_54.

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Crinò, P., A. Lai, F. Saccardo, and A. Scala. "Regeneration of Tomato Shoots from Cotyledonary Explants Challenged by Culture Filtrate of Phytophthora Infestans." In Phytotoxins and Plant Pathogenesis, 443–44. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73178-5_55.

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Davidse, L. C., M. Boekeloo, and A. J. M. van Eggermond. "Elicitation and Suppression of Necrosis in Potato Leaves by Culture Filtrate Components of Phytophthora Infestans." In Biology and Molecular Biology of Plant-Pathogen Interactions, 205–6. Berlin, Heidelberg: Springer Berlin Heidelberg, 1986. http://dx.doi.org/10.1007/978-3-642-82849-2_18.

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Surico, G., A. Evidente, N. S. Iacobellis, and G. Randazzo. "On the Presence and Level of Different Cytokinins in Culture Filtrate of Pseudomonas Syringae Pv. Savastanoi." In Plant Pathogenic Bacteria, 566–70. Dordrecht: Springer Netherlands, 1987. http://dx.doi.org/10.1007/978-94-009-3555-6_115.

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Rahman, Nik Norulaini Abd. "In Vitro Inoculation of Asparagus with Conidial Suspension and Culture Filtrate of Fusarium Oxysporum Schlect F. Asparagt." In Biotechnology in Agriculture, 336–43. Dordrecht: Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1779-1_62.

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Hu, Chunhua, Yuanli Wu, and Ganjun Yi. "Gamma Irradiation of Embryogenic Cell Suspension Cultures from Cavendish Banana (Musa spp. AAA Group) and In Vitro Selection for Resistance to Fusarium Wilt." In Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana, 21–30. Berlin, Heidelberg: Springer Berlin Heidelberg, 2022. http://dx.doi.org/10.1007/978-3-662-64915-2_2.

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AbstractIn this chapter, the establishment of embryogenic cell suspension (ECS) cultures using immature male flowers of triploid banana (Musa AAA Cavendish subgroup cv. ‘Brazil’), followed by somatic embryogenesis and plantlet regeneration is described. Mutation induction is achieved by exposing the ECS to gamma irradiation with the dose of 80 Gy. The mutagenized cell population is transferred to solid long-term suspension culture medium for 96 h to recover from mutagen treatment shock, followed by somatic embryo induction and development medium containing 20% crude culture filtrates from Fusarium oxysporum f. sp. cubense (Foc). After 90 days, the somatic embryos that survive are transferred to the germination medium containing 25% crude culture filtrates. The surviving mature somatic embryos are transferred to rooting medium after the fourth subculture on the germination medium containing 50% crude culture filtrates. Before transplanting in a Foc infected field, the in vitro plantlets are acclimatized and screened for resistance to Foc using a pot-based greenhouse bioassay.
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Boyden, Stephen V., and Ernst Sorkin. "Serological Activity of Various Fractions of Culture Filtrates of the Tubercle Bacillus." In Ciba Foundation Symposium - Experimental Tuberculosis: Bacillus and Host (with an Addendum on Leprosy), 144–62. Chichester, UK: John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470718933.ch11.

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Alam, S. S., and R. N. Strange. "Isolation and Proprties of Two Toxins from Culture Filtrates of Ascochyta Rabiei." In Phytotoxins and Plant Pathogenesis, 385–86. Berlin, Heidelberg: Springer Berlin Heidelberg, 1989. http://dx.doi.org/10.1007/978-3-642-73178-5_34.

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Conference papers on the topic "Culture filtrate"

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Baurina, A. "UTILIZATION OF CULTURE MEDIUM FILTRATE AFTER DEPROTEINIZED SUNFLOWER MEAL FERMENTATION." In 18th International Multidisciplinary Scientific GeoConference SGEM2018. STEF92 Technology, 2018. http://dx.doi.org/10.5593/sgem2018v/6.4/s08.041.

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Lugovtsova, S. Yu, N. A. Neshumaeva, V. Yu Stupko, and N. V. ZobovaN.V. "Root rot toxines as a factor in the selection of resistant forms of oats in vitro." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.153.

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Albehadli, Kadhom, and Ahmed Saeed Mohmed. "Laboratory evaluation of the aphidicidal activity of some entomopathogenic fungal culture filtrate against aphid Schizaphis graminum." In PROCEEDING OF THE 1ST INTERNATIONAL CONFERENCE ON ADVANCED RESEARCH IN PURE AND APPLIED SCIENCE (ICARPAS2021): Third Annual Conference of Al-Muthanna University/College of Science. AIP Publishing, 2022. http://dx.doi.org/10.1063/5.0093653.

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Hemon, A. Farid, Sumarjan, and Abdurrahman Hanafi. "Induction of insensitive peanut somatic embryo to medium containing culture filtrate from various races of Sclerotium rolfsii and plantlet regeneration." In PROCEEDINGS OF THE 2ND INTERNATIONAL CONFERENCE ON BIOSCIENCE, BIOTECHNOLOGY, AND BIOMETRICS 2019. AIP Publishing, 2019. http://dx.doi.org/10.1063/1.5141292.

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Салтанович, Татьяна, Людмила Анточ, and А. Дончилэ. "Оценка реакции мужского гаметофита томата на действие патогенов Alternaria Spp." In International Scientific Symposium "Plant Protection – Achievements and Prospects". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2020. http://dx.doi.org/10.53040/9789975347204.84.

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Research objective: to identify tomato genotypes resistant to Alternaria on variability and symptoms of male gametophyte on selective backgrounds with cultural filtrate of pathogens Alternaria spp. A set of gamete breeding techniques and genetic-statistical analysis were used in the experiments. Some patterns of the variability and heritability of traits in the tomato male gametophyte have been identified on media with filtrates of pathogens. The differences in the resistance of pollen to the filtrate influence were established; the differentiation and selection of genotypes for further breeding were made. These studies can be used at different stages of the selection process.
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Liu, Hua-zhen, Wei Chen, Qi-ying Liu, Xia Zhang, Li-xiu Wang, and Cheng-wu Chi. "A NEW PEPTIDE THROMBIN INHIBITOR FROM STREPTOMYCES GRISEUS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644330.

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A new peptide thrombin inhibitor was found in the Streptomyces griseus strain 254 isolated from a soil sample from Tongan, Fujian province, China, the inhibitor being a secondary metabolic product. The production of the inhibitor reached a maximum after 3 days culture of bacteria at 28°C in a rotary shaker. The inhibitor excreted in the culture filtrate was purified by absorption on macroporous resin, followed by ion exchange chromatography on DEAE-52, CM-32 cellulose, affinity chromatography on the immobilized thrombin and high performance liquid chromatography. The amino acid composition of the inhibitor was determined to be Val(2), Met(l), Ile(l), Leu(2) and Arg (1), similar to that of the amino acid residues around the reactive site of human antithrombin III, the critical plasma inhibitor of thrombin. The NH2-terminal residue of the inhibitor seems to be blocked by the alkyl group due to the negative reaction to ninhydrin, whereas the COO-terminal residue is most likely to be arginal because of that Arg was not found in the amino acid analysis, unless the peptide was oxidized by performic acid before acid hydrolysis. The chromogen substrates Bz-Phe-Val-Arg-PNA and Bz-Gly-Pro-Lys-PNA were used to determine the thrombin and plasmin activities, respectively. Besides thrombin, the purified inhibitor also exhibits a weak inhibitory activities on trypsin and much weak on plasmin, but not on chymotrypsin and other protein-ases.
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Sasco, Elena. "Efectele genetice implicate în răspunsul grăului comun la filtratul de cultură Drechslera sorokiniana (SACC.) subram." In VIIth International Scientific Conference “Genetics, Physiology and Plant Breeding”. Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2021. http://dx.doi.org/10.53040/gppb7.2021.71.

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Helminthosporiosis caused by the fungus Drechslera sorokiniana (Sacc.) causes significant crop and quality losses to Triticum aestivum L. in agroecological conditions with extreme humidity. Increasing the resistance is considered the most cost-effective and sustainable approach to disease control. The aim of this study was to determine the genetic effects involved in the inheritance of resistance, using the ge-netic model of character reproduction in descendants of wheat. Generations F1, F2, BCP1 and BCP2, de-scended from the mutual crossing of the parents Basarabeanca / Moldova 30 and Moldova 30 / Moldova 3 (P1 and P2) were evaluated for the response of callus characters to the action of D. sorokiniana culture filtrate on the medium Murashige Skoog. Fungal metabolites have decreased the effects of gene actions and epistatic interactions, but also their variance. The phenomenon corresponds to the decrease of callus indices. A great importance for the heredity of the character of the surface of the callus manifested the epistatic effects of additive-dominant (ad) type. In the case of callus biomass comparable to the mean val-ues were the a actions, but also the ad and dd epistatic effects. The predominant involvement of epistatic effects indicates the need for resistance selections to helminthosporiosis in late generations of wheat.
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Sasco, Elena. "Variabilitatea patogenității unor agenți fungici ai putregaiului de rădăcină la grâul comun de toamnă." In International Scientific Symposium "Plant Protection – Achievements and Prospects". Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2020. http://dx.doi.org/10.53040/9789975347204.08.

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The study established different reactions of the growth characters of the autumn common wheat seedlings to the treatment of seeds of genotypes Moldova 614, Moldova 66 and L Selania / Accent with the culture filtrates of the strains of Alternaria alternata, Drechslera sorokiniana and Fusarium solani. The 3 strains of the F. solani pathogen produced concomitant repression of root and stem in Moldova 614 and Moldova 66, but differentiated in L Selania / Accent, being identified as the most aggressive in this study. The highest sensitivity was recorded by L Selania / Accent in the case of the root under the action of Alternaria alternata strains.
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9

Mihnea, Nadejda. "Reacția unor linii de tomate la izolatele fungului Alternaria alternata." In VIIth International Scientific Conference “Genetics, Physiology and Plant Breeding”. Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2021. http://dx.doi.org/10.53040/gppb7.2021.64.

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The paper presents the results of the appreciation of some tomato lines based on the resistance to the fungal pathogen Alternaria alternata. Culture filtrates (CF) of pathogen in the most cases did not significantly influence for the seed germination. A more obvious impact was manifested in the case of the root and the stem, the deviations from the control constituting -38.2…. -69.6% for the root and -40.7… -72.6% - for the stem. It was found that the greatest importance in the reaction to A. alternata isolates for seed germination had the genotypic factor, and for root and stem growth - the isolation factor - 95.7-91.0%.
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10

Korolevski, Svetlana. "Vasile Ciocanu și contribuțiile sale istorico-literare." In Conferinta stiintifica nationala cu participare internationala „Lecturi in memoriam acad. Silviu Berejan”. “Bogdan Petriceicu-Hasdeu” Institute of Romanian Philology, Republic of Moldova, 2021. http://dx.doi.org/10.52505/lecturi.2021.05.12.

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Numele istoricului literar Vasile Ciocanu (1942-2003) s-a impus cu pregnanţă în mediul ştiinţific şi cultural din a doua jumătate a secolului trecut şi începutul celui în curs. Consacrat cercetării academice, contribuţiile sale se înscriu în efortul comun de valorificare a moştenirii literare şi de reintegrare a acesteia în circuitul valoric general românesc. Actualizând aspecte mai puţin cunoscute privind viaţa literară a Basarabiei sau parcursul biografic şi de creaţie al unor autori basarabeni din sec. al XIX-lea şi din prima jumătate a sec. al XX-lea, secvenţe privind contactele scriitorilor de peste Prut cu Basarabia, abordările docte, cu date de ordin istorico-cultural scrupulos filtrate, ca şi ediţiile atent îngrijite (C. Stamati, A. Donici, M. Kogălniceanu, C. Stamati-Ciurea, T. Vârnav), constituie un indiciu al probităţii ştiinţifice şi resuscită interesul cercetătorilor, mai cu seamă al celor tineri, pentru epoca preclasică şi clasică a literaturii române.
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