Academic literature on the topic 'Culture de cellules souches mésenchymateuses'
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Journal articles on the topic "Culture de cellules souches mésenchymateuses":
Némos, C., L. Basciano, and A. Dalloul. "Effet et applications potentielles de la culture des cellules souches mésenchymateuses de moelle osseuse en condition d’hypoxie." Pathologie Biologie 60, no. 3 (June 2012): 193–98. http://dx.doi.org/10.1016/j.patbio.2011.07.004.
van Gaver, E., E. Bauer, F. X. Gunepin, A. Fabre, and S. Rigal. "18 - Cellules souches mésenchymateuses humaines : prélèvements à partir de ligaments croisés antéro-externes rompus. Cultures à 30 jours." Revue de Chirurgie Orthopédique et Réparatrice de l'Appareil Moteur 91, no. 8 (December 2005): 62. http://dx.doi.org/10.1016/s0035-1040(05)84524-6.
Sensebé, Luc, and Philippe Bourin. "Cellules souches mésenchymateuses." médecine/sciences 27, no. 3 (March 2011): 297–302. http://dx.doi.org/10.1051/medsci/2011273297.
Borhan, R., J. Lee, G. Youssef, A. Bensussan, and M. Philpott. "Différence de signature génétique moléculaire des cellules du derme papillaire entre le cuir chevelu alopécique et le cuir chevelu non alopécique dans les cultures cellulaires bidimensionnelle, tridimensionnelle et co-culture avec les cellules souches mésenchymateuses dérivées des adipocytes." Annales de Dermatologie et de Vénéréologie 146, no. 12 (December 2019): A247. http://dx.doi.org/10.1016/j.annder.2019.09.388.
Lazennec, Gwendal. "Les cellules souches mésenchymateuses." médecine/sciences 27, no. 3 (March 2011): 285–88. http://dx.doi.org/10.1051/medsci/2011273285.
Ménard, Cédric, and Karin Tarte. "Immunosuppression et cellules souches mésenchymateuses." médecine/sciences 27, no. 3 (March 2011): 269–74. http://dx.doi.org/10.1051/medsci/2011273269.
Maumus, Marie, Yves-Marie Pers, Maxime Ruiz, Christian Jorgensen, and Danièle Noël. "Cellules souches mésenchymateuses et médecine régénératrice." médecine/sciences 34, no. 12 (December 2018): 1092–99. http://dx.doi.org/10.1051/medsci/2018294.
Waszak, P., and B. Thébaud. "Développement pulmonaire et cellules souches mésenchymateuses." Archives de Pédiatrie 18 (July 2011): S86—S91. http://dx.doi.org/10.1016/s0929-693x(11)71096-x.
Jorgensen, Christian, Farida Djouad, Vanessa Fritz, Florence Apparailly, Pascale Plence, and Danielle Noël. "Cellules souches mésenchymateuses et polyarthrite rhumatoïde." Revue du Rhumatisme 70, no. 10-11 (November 2003): 855–57. http://dx.doi.org/10.1016/j.rhum.2003.09.001.
Detante, O. "Cellules souches mésenchymateuses et accident vasculaire cérébral." Transfusion Clinique et Biologique 22, no. 4 (September 2015): 191. http://dx.doi.org/10.1016/j.tracli.2015.06.221.
Dissertations / Theses on the topic "Culture de cellules souches mésenchymateuses":
Martin, Céline. "Étude des procédés d’amplification de cellules souches mésenchymateuses humaines." Thesis, Université de Lorraine, 2016. http://www.theses.fr/2016LORR0261/document.
Progress in regenerative medicines over the past ten years have led to an important research mobilisation, but obtaining a sufficient amount of human stem cells remains nonetheless problematic, especially for mesenchymal stem cells (MSC). Hence, this work developed an approach coupling biology and process engineering to identify barriers limiting MSC growth. The study of scaled-up amplification methods was performed using microcarriers and a 200~mL minibioreactors platform. In order to maximise MSC growth in a biochemically controlled environment, a serum free medium development was tested as well. Human MSC as model cell type for cellular therapies have thus been demonstrated as extremely sensitive to freeze/thaw cycles, temperature variations, subject to premature aging and needing a complex medium enriched in multiple growth and adherence factors. Following this study, several pitfalls might be avoided during MSC process scale-up by integrating the cells biology into the bioreactors' process engineering parameters (heat transfer, hydrodamic stress, adhesion surface)
Ni, Xiaofang. "Culture et différenciation cellulaire sur des substrats structurés et dans des dispositifs microfluidiques." Paris 6, 2009. http://www.theses.fr/2009PA066666.
Yang, Jingwei. "Optimisation de modèles de culture 3D pour la différenciation des cellules souches mésenchymateuses : application à la chondrogénèse." Nancy 1, 2006. http://docnum.univ-lorraine.fr/public/SCD_T_2006_0251_YANG.pdf.
Ln this study, we were interested in the reconstruction of cartilage tissue based on human mesenchymal stem cells (hMSCs). These cells were characterized by phenotype analysis (CD 34- /CD45-/CD166+/CDI66+/CD90+/CD73+), then they were pipetted in 3D culture, rnicromass. We analyzed the typical mesenchymal markers (CDI05, CD166 and CD90) by flow cytometry along 21 days of cultu:e. The differentiation of hMSCs was induced in micromass cultures with a serum-free medium containing TGF-β1 (10ng/ml). We investigated the modification of cell phenotype, analyzed metabolic activity and proliferation. Our experimental results showed a decrease of hMSCs phenotype markers and appearance of eÀiracellular matrix. Furthermare, the analysis of cell cycle revealed a decrease of proliferation activity. We have also defined, in the second part of our study, the culture conditions of a biomaterial (alginate gel) used in cartilage tissue engineering. Different viscosities and concentrations of the biomaterial were monitored. CeIl toxicity and cell proliferation were deterrnined. These results allowed us ta define the experimental condition that will be used in future cartilage tissue engineering study, namely in the effect of mechanical compression
Ferrari, Caroline. "Études cinétiques de procédés d'expansion de cellules souches mésenchymateuses cultivées sur microporteurs en systèmes agités." Thesis, Université de Lorraine, 2012. http://www.theses.fr/2012LORR0117/document.
The extensive use of mesenchymal stem cells (MSC) in tissue engineering increases the necessity to improve the expansion performance. This work aimed at studying an efficient expansion process for porcine MSC in agitated mode. First, a culture medium was adapted to the multipotent porcine MSC. Then, various expansion modes and agitation conditions were evaluated with the cells fixed on microcarriers. Cultures on the Cytodex 1 microcarrier enabled to reach a specific growth rate of 0.54 d-1, which was higher than the one observed in static T-flasks (0.31 d-1), with the same culture conditions. In parallel, an innovative counting method was proposed for the automatic enumeration of cells cultivated on Cytodex 1, without passing by a trypsination step. Finally, the operating conditions of the expansion process were studied. Compared to a culture of MSC on non-agitated Cytodex 1 microcarriers, cell aggregation occurred and an apparent decrease in the cell concentration was observed at an agitation rate of 25 and 75 rpm. Moreover, the addition of microcarriers during a 300 h culture, performed in an agitated culture at 25 rpm and in a volume of 200 mL enabled to prolong the cell proliferation without any aggregation, while maintaining the multipotency of the cells. A cell concentration of 3 x 105 cells/mL was obtained, instead of the 1.2 x 105 cells/mL in static flasks with the same culture conditions. An efficient expansion process for porcine MSC under agitated conditions has therefore been proposed
Basciano, Leticia. "L'effet de l'hypoxie sur les conditions de culture des cellules souches mésenchymateuses de la moelle osseuse." Thesis, Nancy 1, 2011. http://www.theses.fr/2011NAN10100/document.
It is now settled that mesenchymal stem cells (MSC), reside in the same microenvironment or niche than hematopoietic stem cells (HSC), within the bone marrow (BM). It is also known that the O2 tension (pO2) of the niche is below 5% as compared to 21% O2 in the air and 12-15% in the arterial blood. As developed in our recent review, this physiological hypoxia protects stem cells from oxidative stress and maintains their multipotential state. Our hypothesis is that MSC cultured in hypoxia should be closer to their physiological condition and therefore more "multipotent". MSC from human BM were cultured et 21% and at 5% pO2. Their morphology, their ability to differentiate into osteocytes and adipocytes, and their transcriptome were compared at different passages. We observed a decrease of proliferation rate in early times in hypoxia, characterized by inhibition of the expression of genes involved in cell replication and cell cycle, and an increase in later passages. Whatever the passage, the genes encoding adhesion molecules and extracellular matrix are stimulated by hypoxia. At later times, the ability of MSC differentiation is stimulated by hypoxia, the cells look to be more immature and show decreased synthesis of mitochondria. Indeed, hypoxia stimulates the synthesis of plasticity genes according to "Gene Ontology" (GO) terms, and of several genes involved in neuronal- and epithelial-cell development. In conclusion, the culture of MSC from BM in hypoxia seems to be more physiological and may be useful for regenerative medicine applications
Berlier, Jessica. "Identification de facteurs favorisant la survie des cellules souches mésenchymateuses humaines carencées en sérum." Doctoral thesis, Universite Libre de Bruxelles, 2016. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/235574.
Doctorat en Sciences biomédicales et pharmaceutiques (Médecine)
info:eu-repo/semantics/nonPublished
Guerrero, Julien. "Devenir des cellules souches mésenchymateuses humaines dans un environnement tridimensionnel : application à l’ingénierie du tissu osseux." Thesis, Bordeaux, 2014. http://www.theses.fr/2014BORD0200/document.
Bone tissue engineering aims to resolve the existing limitations of boneregeneration methods. One of the proposed strategies consists on the association,within a three-dimensional (3D) matrix, with autologous cells able to regenerate afunctional 3D tissue. The purpose of this study was therefore to investigate theimpact of cellular communication, between cells of the stromal compartment andendothelial cells, within the three-dimensional porous matrix made of biodegradablenatural polysaccharides, focusing on bone repair. Our results show that thearchitecture and the nature of the 3D macroporous matrix promotes the guidance ofmesenchymal stems cells, derived from human bone marrow, towards theosteoblastic lineage. Also, that the organization in aggregates, promoted by the 3Dmatrices, stimulated cell communication, evidenced by the formation of GAPjunctions and activity of Connexins 43. We also focused on the function ofPannexines 1 and 3 for the 3D culture in these matrices of polysaccharides. Inconclusion, this work shows that cell-cell interactions play a major role in order toimprove bone tissue regeneration. Also, cellular and experimental data demonstratesthe advantage of using a total fraction of bone marrow cells to promote both boneformation and vascularization
Thepenier, Cédric. "Optimisation d'un procédé de culture d'épiderme autologue : influence d'un feeder humanisé et d'une faible tension d'oxygène." Paris 7, 2014. http://www.theses.fr/2014PA077083.
To enhance the production conditions for cultured epidermal autografts (CEA) for large burns, we sought to study the in vitro effect of a low oxygen level on epidermal growth. We tested this parameter on CEA grown on murin feeder cells (Green's method) as well as human feeder cells. We first could evidence that the optimal feeder density depended on the oxygen level. A feeder density made optimal at 20% 02 could prove inhibitory on keratinocyte growth at 3% 02. At their respective best feeder densities, low oxygen level (3%) led to an average 4,2 fold increase in keratinocyte yield for a same arrest day as compared with 20% culture. This effect proved to be stable on several successive passagings, showing the increase in proliferation did not take place at the expense of tell self renewal. Keratinocytes grown at a low oxygen level kept their ability to form a stratifying epidermis on an in vivo NOD/SCID mouse excisional model. In parallel, the increase in proliferation was also observed when keratinocytes were cultured on human feeder cells, bone marrow mesenchymal stroma' cells and dermal fibroblasts. This effect of a low oxygen tension on keratinocytes appears to be partly direct, as the growth rate of HaCat feederless keratinocytes was enhanced at 3% vs 20% 02. It is also partly an indirect effect, as conditioned medium from murin feeder cells cultured in hypoxia has a more pronounced positive effect on keratinocyte growth than its normoxic counterpart. These preliminary resuits could lead to the modification on the culture protocol currently in use for the majority of CEA grafts for large burns. The expected benefits for the patients, beyond slightly shortening culture time, would include salvaging abortive cultures and bringing less differentiated keratinocytes, a parameter linked with a decrease in fibrotic evolution on murin models
Mohand, Kaci Faïza. "Bioingénierie des cellules souches mésenchymateuses médullaires cultivées en 3D : application au traitement de l’anévrysme de l’aorte abdominale." Thesis, Paris Est, 2012. http://www.theses.fr/2012PEST0079.
Abdominal aortic aneurysm (AAA) is a degenarative disease of the arterial wall, which is usually treated with a conventional surgery or an andovascular stent. Due to its high morbidity and mortality, the AAA constitutes a major public health concern. The aim of this thesis is to evaluate the imapct of OD culture of mesenchymal stem cells (MSC), in particular on their phenotype, their multipotency, their ability to repair aneurysms in vivo and to acquire a phenotype suitable to the nechanical stress they support in vitro. Optmal culture conditions in a 3D hydrogel of hyaluronic acid preserving the multipotency of MSC in vitro have been established. Under mechanical effects, reproducing those supported by the aortic wall in vivo, 2D and 3D CSM seem to preserve their multipotency. However, under such dynamic conditions, the viability of 3D CSM increases unlike that of 2D CSM. By using a rat xenograft model, the results also show that injection of 2D or 3D CSM, stabilizes the AAA and improves the mechanical strenght of the aneurysmal vessel wall. The study in rat was supplemented by an evaluation of a therapeutic cell-based approach using 3D CSM in the case of chronic false aneurysms of the isthlus in pigs. This step allowed the characterization of 3D CSM and the development of an experimental model in pigs, which allows to consider cell therapy in this model. More genrally, this work contributes to a better understanding of CSM biology and to an improvement of the approaches used in cell therapy and regenerative medicine
Hammoud, Mohammad. "Effet de l’association des basses concentrations d’O2 et des cellules stromales mésenchymateuses sur l’expansion ex vivo des cellules souches et progénitrices hématopoïétiques." Thesis, Besançon, 2012. http://www.theses.fr/2012BESA3008/document.
To optimize at best the hematopoietic engraftment, we suggest in this work to improve the ex vivo expansion conditions by moving them closer to physiology. Indeed, we propose to culture placental CD34+ (HSC/PH) on MSC layer in combination with LO2-C to ensure the amplification of HP together with the maintenance/expansion of HSC. Compared to the single culture and/or atmospheric oxygenation, our experimental model allows a better maintenance of primitive HP (Pre-CFC) and HSC together with a quite good amplification of total cells, CD34+ cells and committed HP despite of lower than control condition. Moreover, exogenous IL-3 shows crucial effect in co-culture at LO2-C (1.5% O2) since its addition better preserves and even increases the number of HSC compared to the CD34+ cells control from D0. We then studied the secretion of soluble factors in culture supernatants and found that IL-6, VEGF and IL-8 were present in larger quantities at LO2-C in both co-culture and MSC culture. Finally, the CD146, CD49a, CD54, CD200 and CD105 membrane antigens appear to be up-regulated in MSCs when incubated at 5% O2. However, the involvement of these factors and antigens in paracrine effect and/or direct cell to cell contact mechanisms at LO2-C requires further investigations. In conclusion, the combination of LO2-C and MSC would be promising in the field of HSC/PH grafts expansion to achieve its main objective of reducing the post-transplant cytopenia period together with maintaining the long-term graft potential
Books on the topic "Culture de cellules souches mésenchymateuses":
Ian, Freshney R., Pragnell Ian B, and Freshney Mary G, eds. Culture of hematopoietic cells. New York: Wiley-Liss, 1994.
S, Odorico Jon, Zhang S. -C, and Pedersen Roger A, eds. Human embryonic stem cells. Abingdon, Oxon, UK: Garland Science/BIOS Scientific Publishers, 2006.
Hescheler, Jürgen, and Erhard Hofer. Adult and Pluripotent Stem Cells: Potential for Regenerative Medicine of the Cardiovascular System. Springer, 2014.
Hescheler, Jürgen, and Erhard Hofer. Adult and Pluripotent Stem Cells: Potential for Regenerative Medicine of the Cardiovascular System. Springer London, Limited, 2014.
Hescheler, Jürgen, and Erhard Hofer. Adult and Pluripotent Stem Cells: Potential for Regenerative Medicine of the Cardiovascular System. Springer, 2016.
Prasad, Mayuri. Innovative Strategies in Tissue Engineering. River Publishers, 2015.
Nardo, Paolo Di, and Mayuri Prasad. Innovative Strategies in Tissue Engineering. River Publishers, 2022.
Nardo, Paolo Di, and Mayuri Prasad. Innovative Strategies in Tissue Engineering. River Publishers, 2015.
Nardo, Paolo Di, and Mayuri Prasad. Innovative Strategies in Tissue Engineering. River Publishers, 2022.
Elçin, Y. Murat. Tissue Engineering, Stem Cells and Gene Therapies. Springer, 2003.
Book chapters on the topic "Culture de cellules souches mésenchymateuses":
Rosset, P. "Cellules souches mésenchymateuses." In Réparations tissulaires à la jambe, 131–37. Paris: Springer Paris, 2012. http://dx.doi.org/10.1007/978-2-287-99066-3_13.
Conference papers on the topic "Culture de cellules souches mésenchymateuses":
Le Choismier, H. "Un transporteur d’oxygène universel d’origine marine au service de la santé." In 66ème Congrès de la SFCO. Les Ulis, France: EDP Sciences, 2020. http://dx.doi.org/10.1051/sfco/20206601009.
Bouvet-Gerbettaz, S., GF Carle, and N. Rochet. "Ingénierie tissulaire osseuse et cellules souches mésenchymateuses : applications à la sphère buccale et maxillo-faciale." In 54ème Congrès de la SFMBCB. Les Ulis, France: EDP Sciences, 2011. http://dx.doi.org/10.1051/sfmbcb/20115403016.
Casteilla, L. "Les cellules souches mésenchymateuses de la moelle osseuse et des tissus adipeux : de la physiologie à la médecine régénératrice." In 63ème Congrès de la SFCO, edited by S. Boisramé, S. Cousty, J. C. Deschaumes, V. Descroix, L. Devoize, P. Lesclous, C. Mauprivez, and T. Fortin. Les Ulis, France: EDP Sciences, 2015. http://dx.doi.org/10.1051/sfco/20156301001.
Coffin, E., A. Brun, G. Perrod, M. Piffoux, I. Boucenna, M. Bruzzi, L. M'harzi, et al. "Prévention des sténoses oesophagiennes après dissection sous-muqueuse étendue par application de gel Pluronic F127 associé ou non à des vésicules extracellulaires de cellules souches mésenchymateuses dans le modèle porcin." In Journées Francophones d'Hépato-Gastroentérologie et d'Oncologie Digestive (JFHOD). Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-1680886.