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Omelyanenko, Vitaliy, Inna Semenets-Orlova, Olena Khomeriki, Lyudmyla Lyasota, and Yuliia Medviedieva. "Technology transfer management culture (education-based approach)." Problems and Perspectives in Management 16, no. 3 (September 20, 2018): 454–63. http://dx.doi.org/10.21511/ppm.16(3).2018.36.
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Research deals with the analysis of theoretical aspects of increasing the competence of scientists and experts, whose task is to work with technology as a good promotion of technology on the market with a view to its commercialization or search for technological solutions according to the tasks of the organization and to examine the startup methodology as a tool of improving the technology transfer skills efficiency. The new specifics of technology transfer management within the Education 3.0 paradigm are considered. In the paper, the authors analyze the technology transfer as important source of innovation creation and an integral part of business development. Target result of technologies introduction, implemented within limited period with limited resources as an innovative purpose of technology transfer, which is aimed at the best possible development of organization, is identified. The paper also suggests consider¬ing processes of technology development and technology transfer based on startup methodology.
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Morris, M. C., L. Morrison, M. A. Joyce, and B. Rabel. "Trapping sheep blowflies with lures based on bacterial cultures." Australian Journal of Experimental Agriculture 38, no. 2 (1998): 125. http://dx.doi.org/10.1071/ea97088.
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Summary. Baits of Proteus mirabilis cultured in a commercial medium or in a gut mucus mixture were tested in New Zealand sheep pasture for their ability to trap sheep strike flies. Liver–sodium sulfide baits were used for comparison. At the start of the flystrike season, medium cultures were as effective as liver–sodium sulfide baits in trapping flies. As the season progressed, the liver–sodium sulfide mixture trapped a significantly higher number of Lucilia cuprina and Calliphora stygia than medium cultures, though they trapped significantly fewer Chrysomya rufifacies. In one case the medium culture trapped a significantly higher proportion of gravid L. cuprina than the liver–sodium sulfide mixture. Adding dimethyl sulfoxide to the medium culture significantly reduced its efficacy. The gut mucus culture was significantly less effective than the liver–sodium sulfide bait in trapping gravid L. cuprina, and significantly more effective in trapping Chrysomya rufifacies. This is the first published record of sheep strike flies being attracted by bacterial odours in the field.
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Rafiqa, Rafiqa, and Ahmad Munawir. "Mandar Culture-Based English Education." ELT Worldwide: Journal of English Language Teaching 4, no. 2 (November 18, 2017): 153. http://dx.doi.org/10.26858/eltww.v4i2.4497.
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The purpose of this article is to describe a model of English language learning based on Mandar culture. Descriptive method was used in this study, the aim of it to describe a contextual learning model so the students are able to speak English and still preserve Mandar culture. The purposive sampling technique was used to determine the sample of the research, all of the students of English Education Program in 2016 academic year. Data were collected by using observation technique, questionnaire and documentation. This study described about English language learning based on Mandar culture. It was a process of language learning combined with local culture, namely Mandar culture. This study incorporates elements of Mandar culture in the learning process with the aim that students can understand and preserve their own culture and can add their English vocabulary. There are six Mandar cultural themes in Mandar culture-based language learning, namely malatigi (Mandar customary ritual on the night of marriage), mandarese marriage, sayyang pattuddu ', sayyang-sayyang, fisherman party (Sandeq), and panette' (making Mandar silk sarong). Applying Mandar cultures in English language learning can improve English language students and the students can preserve Mandar culture and increase student interest and critical thinking skills.
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Zeng, Li, Nan Wu, and Baihan Li. "The Soft Power of Culture: Economics Consideration Based on the Perspective of “Three Forces” of Culture." Asia Social Science Academy 8, no. 2 (August 30, 2022): 93–107. http://dx.doi.org/10.51600/jass.2022.8.2.95.
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Focusing on “three forces” of cultural productivity, cultural competitiveness and cultural soft power, the paper considers “three forces” of culture from an economics perspective by constructing a linkage mechanism model of “three forces” of culture. From cultural resources to cultural capital into cultural productivit y, cultural productivity can be optimized through the balance between supply and demand. Then, the paper analyzes the construction of cultural competitiveness from perspective of cultural inheritance and innovation affecting the balance between supply and demand, efficiency. Combined with Michael Porter Diamond Model, it analyzes six factors that affect “three forces” of culture, such as enterprise strategy, related industries, demand conditions, production factors, opportunities and government. Therefore, “three forces” of culture mechanism aims at realizing the linkage mechanism of cultural economic value, cultural mechanism and social value, thus enhancing cultural soft power and national image.
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Sansupa, Chakriya, Sara Fareed Mohamed Wahdan, Terd Disayathanoowat, and Witoon Purahong. "Identifying Hidden Viable Bacterial Taxa in Tropical Forest Soils Using Amplicon Sequencing of Enrichment Cultures." Biology 10, no. 7 (June 22, 2021): 569. http://dx.doi.org/10.3390/biology10070569.
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This study aims to estimate the proportion and diversity of soil bacteria derived from eDNA-based and culture-based methods. Specifically, we used Illumina Miseq to sequence and characterize the bacterial communities from (i) DNA extracted directly from forest soil and (ii) DNA extracted from a mixture of bacterial colonies obtained by enrichment cultures on agar plates of the same forest soil samples. The amplicon sequencing of enrichment cultures allowed us to rapidly screen a culturable community in an environmental sample. In comparison with an eDNA community (based on a 97% sequence similarity threshold), the fact that enrichment cultures could capture both rare and abundant bacterial taxa in forest soil samples was demonstrated. Enrichment culture and eDNA communities shared 2% of OTUs detected in total community, whereas 88% of enrichment cultures community (15% of total community) could not be detected by eDNA. The enrichment culture-based methods observed 17% of the bacteria in total community. FAPROTAX functional prediction showed that the rare and unique taxa, which were detected with the enrichment cultures, have potential to perform important functions in soil systems. We suggest that enrichment culture-based amplicon sequencing could be a beneficial approach to evaluate a cultured bacterial community. Combining this approach together with the eDNA method could provide more comprehensive information of a bacterial community. We expected that more unique cultured taxa could be detected if further studies used both selective and non-selective culture media to enrich bacteria at the first step.
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McIntyre, Roger P., Martin S. Meloche, and Jane M. Lang. "Culture-Based Market Segmentation." Journal of Euromarketing 2, no. 1 (March 11, 1993): 31–47. http://dx.doi.org/10.1300/j037v02n01_04.
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Huang, Jieguan. "The Classification of Folk Culture Based on Multicharacteristics." Mobile Information Systems 2022 (June 8, 2022): 1–8. http://dx.doi.org/10.1155/2022/7949267.
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China is an ancient country with a long history. It is composed of many ethnic groups and has formed a variety of unique folk cultures. China has many cultures where each region has its own cultural forms. The diversity and complexity of the Chinese folk culture are locality intertwined. The folk culture with Chinese characteristics and local characteristics deserves attention. The full integration of folk culture into Chinese modern and contemporary literary works can effectively enhance the cultural connotation of literary works and play a very important role in promoting the development of our country’s culture, and at the same time, it can also stimulate readers’ interest in reading in the later period. Further understanding of modern Chinese folk culture is of unprecedented help. Therefore, this paper takes “the analysis of the characteristics of folk culture in Chinese modern and contemporary literary works” as a research perspective, hoping to more effectively promote the integration of folk culture and Chinese literary works through this research and then promote the coordinated development and improvement of the two.
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Niazian, M., S. A. Sadat Noori, P. Galuszka, and S. M. M. Mortazavian. "Tissue culture-based Agrobacterium-mediated and in planta transformation methods." Czech Journal of Genetics and Plant Breeding 53, No. 4 (November 10, 2017): 133–43. http://dx.doi.org/10.17221/177/2016-cjgpb.
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Gene transformation can be done in direct and indirect (Agrobacterium-mediated) ways. The most efficient method of gene transformation to date is Agrobacterium-mediated method. The main problem of Agrobacterium-method is that some plant species and mutant lines are recalcitrant to regeneration. Requirements for sterile conditions for plant regeneration are another problem of Agrobacterium-mediated transformation. Development of genotype-independent gene transformation method is of great interest in many plants. Some tissue culture-independent Agrobacterium-mediated gene transformation methods are reported in individual plants and crops. Generally, these methods are called in planta gene transformation. In planta transformation methods are free from somaclonal variation and easier, quicker, and simpler than tissue culture-based transformation methods. Vacuum infiltration, injection of Agrobacterium culture to plant tissues, pollen-tube pathway, floral dip and floral spray are the main methods of in planta transformation. Each of these methods has its own advantages and disadvantages. Simplicity and reliability are the primary reasons for the popularity of the in planta methods. These methods are much quicker than regular tissue culture-based Agrobacterium-mediated gene transformation and success can be achieved by non-experts. In the present review, we highlight all methods of in planta transformation comparing them with regular tissue culture-based Agrobacterium-mediated transformation methods and then recently successful transformations using these methods are presented.
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Wijayawardene, Nalin N., Mohammad Bahram, Iván Sánchez-Castro, Dong-Qin Dai, Kahandawa G. S. U. Ariyawansa, Udeni Jayalal, Nakarin Suwannarach, and Leho Tedersoo. "Current Insight into Culture-Dependent and Culture-Independent Methods in Discovering Ascomycetous Taxa." Journal of Fungi 7, no. 9 (August 28, 2021): 703. http://dx.doi.org/10.3390/jof7090703.
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Culture techniques are vital in both traditional and modern fungal taxonomy. Establishing sexual–asexual links and synanamorphs, extracting DNA and secondary metabolites are mainly based on cultures. However, it is widely accepted that a large number of species are not sporulating in nature while others cannot be cultured. Recent ecological studies based on culture-independent methods revealed these unculturable taxa, i.e., dark taxa. Recent fungal diversity estimation studies suggested that environmental sequencing plays a vital role in discovering missing species. However, Sanger sequencing is still the main approach in determining DNA sequences in culturable species. In this paper, we summarize culture-based and culture-independent methods in the study of ascomycetous taxa. High-throughput sequencing of leaf endophytes, leaf litter fungi and fungi in aquatic environments is important to determine dark taxa. Nevertheless, currently, naming dark taxa is not recognized by the ICN, thus provisional naming of them is essential as suggested by several studies.
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Mirzaei, Zainab. "Organization of Islamic Culture Based Culture of Work Ethic." Academic Journal of Research in Economics and Management 2, no. 3 (March 2014): 31–37. http://dx.doi.org/10.12816/0006540.
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Choi, Yoon Young, Jin-I. Seok, and Dong-Sik Kim. "Flow-Based Three-Dimensional Co-Culture Model for Long-Term Hepatotoxicity Prediction." Micromachines 11, no. 1 (December 27, 2019): 36. http://dx.doi.org/10.3390/mi11010036.
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We developed concave microwell arrays to establish a size-controllable 3-D co-culture liver model for in vitro drug toxicity testing, to predict hepatotoxicity. The interaction of hepatocytes with hepatic stellate cells (HSCs) was investigated by co-culturing primary 3-D hepatocyte spheroids and HSCs (heterosphere), using 3-D liver-on-a-chip. The effect of HSCs was investigated during spheroid formation; they were involved in controlling the organization of spheroidal aggregates and the formation of tight cell–cell contacts. Scanning electron microscopy (SEM) images showed that co-cultured spheroids with smoother surfaces in the flow chip aggregated more tightly and rapidly, compared to mono-cultured spheroids, until 13 days. Metabolic function analysis revealed that heterospheres secreted 40% more albumin and urea than hepatospheres on day 13. Additionally, an acetaminophen (AAP) and isoniazid (INH) concentration-dependent increase in CYP3A4 expression was detected in the 3-D cultures, and an increase in Lactate dehydrogenase (LDH) release after AAP and INH treatment was observed. CYP1A2, Mrp1 and UGT1A5 mRNA expression levels in the heterospheres and hepatospheres were evaluated from days 3 to 13. To examine the potential for toxicity testing in the flow-conditioned culture of the heterospheres, we evaluated cytotoxicity using the endpoint LDH release in the heterospheres and hepatospheres. IC50 values for AAP and INH after 24 h of exposure were calculated from the dose–response curves of the compounds. Flow-conditioned heterosphere culture results suggest that it may be suitable for long-term culture and cytotoxicity testing. Thus, our co-culture system closely resembles the in vivo environment and allows long-term in vitro hepatotoxicity prediction.
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Borgo, Stefano, and Enrico Blanzieri. "Trait-Based Module for Culturally-Competent Robots." International Journal of Humanoid Robotics 16, no. 06 (December 2019): 1950028. http://dx.doi.org/10.1142/s0219843619500282.
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Robots might not act according to human expectations if they cannot anticipate how people make sense of a situation and what behavior they consider appropriate in some given circumstances. In many cases, understanding, expectations and behavior are constrained, if not driven, by culture, and a robot that knows about human culture could improve the quality level of human–robot interaction. Can we share human culture with a robot? Can we provide robots with formal representations of different cultures? In this paper, we discuss the (elusive) notion of culture and propose an approach based on the notion of trait which, we argue, permits us to build formal modules suitable to represent culture (broadly understood) in a robot architecture. We distinguish the types of traits that such modules should contain, namely behavior, knowledge, rule and interpretation traits, and how they could be organized. We identify the interpretation process that maps situations to specific knowledge traits, called scenarios, as a key component of the trait-based culture module. Finally, we describe how culture modules can be integrated in an existing architecture, and discuss three use cases to exemplify the advantages of having a culture module in the robot architecture highlighting surprising potentialities.
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Morabito, Caterina, Nathalie Steimberg, Giovanna Mazzoleni, Simone Guarnieri, Giorgio Fanò-Illic, and Maria A. Mariggiò. "RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes onIn Vitro3D Neuroglial Cell Cultures." BioMed Research International 2015 (2015): 1–14. http://dx.doi.org/10.1155/2015/754283.
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We propose a human-derived neuro-/glial cell three-dimensionalin vitromodel to investigate the effects of microgravity on cell-cell interactions. A rotary cell-culture system (RCCS) bioreactor was used to generate a modelled microgravity environment, and morphofunctional features of glial-like GL15 and neuronal-like SH-SY5Y cells in three-dimensional individual cultures (monotypic aggregates) and cocultures (heterotypic aggregates) were analysed. Cell survival was maintained within all cell aggregates over 2 weeks of culture. Moreover, compared to cells as traditional static monolayers, cell aggregates cultured under modelled microgravity showed increased expression of specific differentiation markers (e.g., GL15 cells: GFAP, S100B; SH-SY5Y cells: GAP43) and modulation of functional cell-cell interactions (e.g., N-CAM and Cx43 expression and localisation). In conclusion, this culture model opens a wide range of specific investigations at the molecular, biochemical, and morphological levels, and it represents an important tool forin vitrostudies into dynamic interactions and responses of nervous system cell components to microgravity environmental conditions.
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Martinez, J. Andres, Matheo Delvenne, Lucas Henrion, Fabian Moreno, Samuel Telek, Christian Dusny, and Frank Delvigne. "Controlling microbial co-culture based on substrate pulsing can lead to stability through differential fitness advantages." PLOS Computational Biology 18, no. 10 (October 31, 2022): e1010674. http://dx.doi.org/10.1371/journal.pcbi.1010674.
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Microbial consortia are an exciting alternative for increasing the performances of bioprocesses for the production of complex metabolic products. However, the functional properties of microbial communities remain challenging to control, considering the complex interaction mechanisms occurring between co-cultured microbial species. Indeed, microbial communities are highly dynamic and can adapt to changing environmental conditions through complex mechanisms, such as phenotypic diversification. We focused on stabilizing a co-culture of Saccharomyces cerevisiae and Escherichia coli in continuous cultures. Our preliminary data pointed out that transient diauxic shifts could lead to stable co-culture by providing periodic fitness advantages to the yeast. Based on a computational toolbox called MONCKS (for MONod-type Co-culture Kinetic Simulation), we were able to predict the dynamics of diauxic shift for both species based on a cybernetic approach. This toolbox was further used to predict the frequency of diauxic shift to be applied to reach co-culture stability. These simulations were successfully reproduced experimentally in continuous bioreactors with glucose pulsing. Finally, based on a bet-hedging reporter, we observed that the yeast population exhibited an increased phenotypic diversification process in co-culture compared with mono-culture, suggesting that this mechanism could be the basis of the metabolic fitness of the yeast.
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Huang, Lihua. "The Fusion of Buddhist Naga Culture Based on Goddesses in China and Japan." International Journal of Education and Humanities 10, no. 2 (August 29, 2023): 21–22. http://dx.doi.org/10.54097/ijeh.v10i2.11321.
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Based on the globalization of nowadays, there are many cultures fusion together. However, the Naga culture in different countries has a distinguished meaning from the different regions. Then there is a debate that some nations believe this is their culture. For example, the Chinese always called themselves the descendants of the dragon, and the Japanese think it may be the origin of their culture based on their history book Records of Ancient Matters. Despite this, other places like Thailand and Malaysia also liked the Naga much in their cultures. So what's the actual Buddhism Naga culture belong to? Is the Buddhism Naga culture, with the fusion of their local religions and becoming a new belief system in those regions? This research paper will focus on the Buddhism Naga culture in China and Japan to discuss the Naga culture with the fusion of their local religions or thoughts based on goddesses.
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Olofsson, Karl, Valentina Carannante, Madoka Takai, Björn Önfelt, and Martin Wiklund. "Ultrasound-Based Scaffold-Free Core-Shell Multicellular Tumor Spheroid Formation." Micromachines 12, no. 3 (March 20, 2021): 329. http://dx.doi.org/10.3390/mi12030329.
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In cancer research and drug screening, multicellular tumor spheroids (MCTSs) are a popular model to bridge the gap between in vitro and in vivo. However, the current techniques to culture mixed co-culture MCTSs do not mimic the structural architecture and cellular spatial distribution in solid tumors. In this study we present an acoustic trapping-based core-shell MCTSs culture method using sequential seeding of the core and shell cells into microwells coated with a protein repellent coating. Scaffold-free core-shell ovarian cancer OVCAR-8 cell line MCTSs were cultured, stained, cleared and confocally imaged on-chip. Image analysis techniques were used to quantify the shell thickness (23.2 ± 1.8 µm) and shell coverage percentage (91.2 ± 2.8%). We also show that the shell thickness was evenly distributed over the MCTS cores with the exception of being slightly thinner close to the microwell bottom. This scaffold-free core-shell MCTSs formation technique and the analysis tools presented herein could be used as an internal migration assay within the MCTS or to form core-shell MCTS co-cultures to study therapy response or the interaction between tumor and stromal cells.
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Uchida, Yasunobu. "Psychological Culture based on Learning." TRENDS IN THE SCIENCES 3, no. 11 (1998): 43–46. http://dx.doi.org/10.5363/tits.3.11_43.
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Stampley, Cheryl. "Social Workers' Culture-Based Countertransferences." Journal of Ethnic & Cultural Diversity in Social Work 17, no. 1 (May 8, 2008): 37–59. http://dx.doi.org/10.1080/15313200801906401.
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., Chris Batara. "VIRTUAL’S CULTURE BASED INFORMATION TECHNOLOGY." International Journal of Research in Engineering and Technology 04, no. 10 (October 25, 2015): 135–40. http://dx.doi.org/10.15623/ijret.2015.0410024.
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Nazir, Asad, Sibylle Enz, Mei Yii Lim, Ruth Aylett, and Alison Cawsey. "Culture–personality based affective model." AI & SOCIETY 24, no. 3 (June 23, 2009): 281–93. http://dx.doi.org/10.1007/s00146-009-0217-2.
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Haselager, Marco, Eduard Perelaer, Arnon P. Kater, and Eric Eldering. "Development of a Novel Lymph Node-Based 3D Culture System Promoting Chronic Lymphocytic Leukemia Proliferation and Survival." Blood 136, Supplement 1 (November 5, 2020): 47–48. http://dx.doi.org/10.1182/blood-2020-141962.
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INTRODUCTION. Primary chronic lymphocytic leukemia (CLL) cells, despite originating from a proliferative disease, rapidly undergo apoptosis in vitro in absence of microenvironmental survival signals1. Although co-culture with stromal cells or the addition of soluble factors can increase and extend CLL survival, no system permits the long-term expansion of CLL cells in vitro2. The difficulties of mimicking a physiologic microenvironment supporting CLL cells hinder in vitro studies of proliferation, drug screens and prevent propagation of rare subclones. For other cancers, various types of 3D cultures have been introduced utilizing scaffolds, gels, spheroid cultures and fluidic systems, representing a more accurate representation of the in vivo microenvironment3. Unlike solid tumors, secondary lymphoid tissues where CLL cells proliferate in vivo, do not derive from a single stem cell progenitor. Developing an appropriate 3D in vitro culture system for CLL is of obvious importance and may contribute pathophysiological relevance to study long-term CLL proliferation and more accurate drug screening4,5. Within the field of CLL, attempts have focused on bone marrow stroma, but it may be biologically and clinically more relevant to investigate the lymph node niche as this is the critical site of CLL proliferation6. METHODS. Primary CLL cells were cultured in various 3D systems including hydrogels, hanging drop cultures and ultra-low attachment plates (ULA) plates in parallel to an optimal 2D system, consisting of the culture of primary CLL cells on a monolayer of CD40L-presenting fibroblasts (3T40) or 3T3 negative control fibroblasts. CLL cells were either cultured as PBMCs alone, with or without T cells, or co-cultured with 3T40 or primary lymph node fibroblasts. CLL cells were either stimulated directly with IL-2, IL-15, IL-21 and CpG and/or indirectly via a T cell stimulation of anti-CD3/CD28. RESULTS. After testing and comparing multiple systems for the in vitro culture of CLL cells, we optimized a novel CLL culture system utilizing ULA plates creating spheroids of PBMCs isolated from peripheral blood. Without the addition of soluble factors or stroma, primary CLL cells in the ULA 3D model could be maintained in culture for 6 weeks as opposed to 1 week in the 2D system. Aside from significantly promoting CLL survival, cultures could be expanded approximately 3-4-fold over a course of 6 weeks using the ULA 3D model. 3D cultures showed a more consistent and significantly increased CLL proliferation compared to 2D cultures, independent of IGHV mutation status, increasing the average proliferation index of 2.87 to 3.90 (n=10). Additionally, co-culture with LN-derived stromal cells further increased CLL proliferation, reaching a maximum of 8 generations (n=6) (Figure 1). Lastly, when PBMCs were stimulated with IL-2, IL-15, IL-21 and CpG, spheroids developed proliferation center-like structures after 4 weeks of culture. CONCLUSIONS. We established a lymph node-based 3D in vitro culture system for CLL leading to increased CLL proliferation and survival compared to 2D systems. The set-up allows long-term expansion of CLL cells in vitro, as well as formation of proliferation center-like structures. We are currently optimizing drug resistance studies, expansion of specific CLL subclones and performing competition experiments. References: 1. Hamilton et al., Mimicking the tumour microenvironment: three different co-culture systems induce a similar phenotype but distinct proliferative signals in primary chronic lymphocytic leukaemia cells, 2012. 2. Asslaber et al., Mimicking the microenvironment in chronic lymphocytic leukaemia - where does the journey go?, 2013. 3. Gurski et al., 3D Matrices for Anti-Cancer Drug Testing and Development, 2010. 4. Nunes et al., 3D tumor spheroids as in vitro models to mimic in vivo human solid tumors resistance to therapeutic drugs, 2019. 5. Aljitwai et al., A novel three-dimensional stromal-based model for in vitro chemotherapy sensitivity testing of leukemia cells, 2014. 6. Van Gent et al., In vivo dynamics of stable chronic lymphocytic leukemia inversely correlates with somatic hypermutation levels and suggest no major leukemic turnover in bone marrow, 2008. Disclosures Kater: Genentech: Research Funding; Abbvie: Research Funding; Roche: Research Funding; Janssen: Research Funding; Celgene: Research Funding. Eldering:Celgene: Research Funding; Janssen: Research Funding; Genentech: Research Funding.
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Setiawan, Alexander, Andreas Handojo, and Rendra Hadi. "Indonesian Culture Learning Application based on Android." International Journal of Electrical and Computer Engineering (IJECE) 7, no. 1 (February 1, 2017): 526. http://dx.doi.org/10.11591/ijece.v7i1.pp526-535.
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<p>Indonesia is a country that has a wealth of diverse cultures spread from Sabang to Merauke. Even though the government has included a lesson about Indonesian culture in terms of some subjects such as Art and Culture, but there are still many people including some students who do not know the forms and names of the tribes, traditional houses, traditional clothing, folk songs, traditional foods, local weapons, and musical instruments regions in Indonesia. Therefore, a learning application is created that utilizes mobile learning technology. Through the use of mobile learning, user can access learning content without any borders so that it can be accessed at any time so that it can be accessed at any time with interesting illustration. Indonesia National Culture Application is made useful to help users in learning the introduction of Indonesian culture that provides the knowledge with illustrations in the form of images, audio, text and video. This application also can be functioned to learn, answer quiz, to view a gallery of images and video and to do puzzle games. The achieved result is an application that can provide knowledge to the users so they can know the culture in Indonesia through the illustrations provided well.</p>
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Puzderová, Štefánia. "REPORT ON CASE-BASED STUDY OF CULTURE-FORMING ELEMENTS WITHIN SELECTED COMPANY CULTURE." KNOWLEDGE - International Journal 54, no. 5 (September 30, 2022): 745–50. http://dx.doi.org/10.35120/kij5405745p.
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This paper is concerned with communication and culture-forming elements within companies. Thepurpose of this paper is to report on the study that was conducted in order to decide whether culture-formingelements of company culture in the Slovak business context have a considerable impact on job satisfaction of theemployees. The most crucial concepts of this paper are the culture-forming elements and the term job satisfaction.The role of language and culture is closely explained in relation to these core concepts. This paper also drawsattention to intercultural communication, company culture, job satisfaction and it maps current situation within thefield emphasising the strong influence of the American tradition of the corporate culture. It examines mutualinfluence of the values on the national and organizational levels. Findings are set forth based on the case-based studyanalysis of company culture enhanced with a data-driven approach. This in-depth analysis of company cultureserves as a sample confirming the interrelationship between the culture-forming elements and job satisfaction and itexplores tendencies and strategies such as taking control of fluctuation and job performance. Besides, a uniquelinking between the language element and other elements such as vision has been recognized. These insightsexemplify strong dependence on the culture-forming elements and job satisfaction. The findings reported in thispaper refer to the differences in the perception of culture-forming elements and it also examines the origin of variouscompany-culture elements comparing trends to American corporate culture. This paper addresses the formation ofcompany culture in terms of knowledge from Communication studies, Cultural studies, Intercultural Communicationand Sociolinguistics and recommends transferring knowledge from these fields into practical applications. Insightshighlighted in this paper can help professionals from Human resources and Communication departments whenforming and re-building attractive company cultures strengthening employee job satisfaction whereby it highlights aneed for interdisciplinary cooperation in order to transfer the knowledge from these scientific fields into practicalapplication and let modern society benefit from the contemporary findings from Humanities. Based on this culturalresearch, this paper does not only offer recommendations for the selected company, but it brings contributions interms of company culture. The outcome of this thesis contributes highly to the best practices shared within businessstudies and provides the east innovation efforts in Slovakia with ways to empower their company cultures and so tomotivate their employees and it is believed that application is not bound only to Slovak business context.
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Torino, Stefania, Brunella Corrado, Mario Iodice, and Giuseppe Coppola. "PDMS-Based Microfluidic Devices for Cell Culture." Inventions 3, no. 3 (September 6, 2018): 65. http://dx.doi.org/10.3390/inventions3030065.
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Microfluidic technology has affirmed itself as a powerful tool in medical and biological research by offering the possibility of managing biological samples in tiny channels and chambers. Among the different applications, the use of microfluidics for cell cultures has attracted much interest from scientists worldwide. Traditional cell culture methods need high quantities of samples and reagents that are strongly reduced in miniaturized systems. In addition, the microenvironment is better controlled by scaling down. In this paper, we provide an overview of the aspects related to the design of a novel microfluidic culture chamber, the fabrication approach based on polydimethylsiloxane (PDMS) soft-lithography, and the most critical issues in shrinking the size of the system.
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Dauksza, Agnieszka. "Affective Diffusion Between Migrants and Inhabitants. Art Based on Migrant Movement." Open Cultural Studies 2, no. 1 (October 1, 2018): 262–72. http://dx.doi.org/10.1515/culture-2018-0024.
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Abstract The main objective of this essay is to consider Polish perception of the 21st-century European movement of migration and analyse the literary and artistic representations of this experience through the examples of Jarosław Mikołajewski’s A Great Surge, Krzysztof Wodiczko’s Guests, Anna Konik’s In the Same City, Under the Same Sky and Margot Sputo’s Who Where?. I relate these works to the latest international art, including projects by Ai Weiwei, E. B. Itso, Giacomo Sferlazzo, and look for common denominators. I claim that the common feature of many artistic projects is the creation of a substitute for contact between the viewer and refugees, appealing to other senses than sight, especially to the sense of touch. These works do not attempt to represent the experience of migrants as individuals, but point to the experience of migration in its elemental aspect-matter and physical contact, exposure to destruction, dependence, intimacy, confrontation with others. What’s more, art based on migrant movement-referring to the imagined experience of direct contact with migrants or their belongings-bypasses the process of identification and empathy. Instead, it relies on affective transmission. I propose a category of affective diffusion as a process that can occur not only between sentient individuals but also as a result of the material aspect of art and the contact with the viewer. The inspiration to create the concept of affective diffusion is the relationship between migrants and inhabitants described by Jarosław Mikołajewski in his essay Wielki Przypływ [A Great Surge] which shows aspects of migration through Lampedusa Island. Many inhabitants who are asked about migrants show intensive emotions like anger, fear and xenophobia. But the testimonies of people who see the living and the dead day after day suggest that these emotions get weaker under the influence of physical contact. It seems that the moment of two bodies getting in touch undermines the frozen system of ideas and emotions, stimulates feelings, opens up a new, ambiguous potential for understanding. This is because expressive emotions lose their importance with the activation of affective transmission. This physical contact gives rise to changes in the economy of feeling, thinking and judging.
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Ardielli, Eva. "Participation of EU Member States’ Citizens on Foreign Culture and Cultural Products." INTERNATIONAL JOURNAL OF INNOVATION AND ECONOMIC DEVELOPMENT 5, no. 3 (2019): 58–64. http://dx.doi.org/10.18775/ijied.1849-7551-7020.2015.53.2005.
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Culture is an important topic of the present time. It is at the heart of contemporary debates about identity, social cohesion, and the development of a knowledge-based economy. The international organizations, like UNESCO, OECD, Council of Europe, as well as European Commission are affirming, that respect for the diversity of cultures, tolerance, dialogue and cooperation, in a climate of mutual trust and understanding, are among the best guarantees of international peace and security. Therefore the cultural and creative sectors are considered as very important in European Union countries. The article deals with the issue of the participation of EU citizens on foreign cultures and cultural product as the expression of interest and openness of the citizens of individual EU member countries to get known foreign culture of other EU member state. The willingness of EU citizens to participate in foreign cultures across EU is evaluated based on the indicators describing the level of foreign languages knowledge and the participation in foreign cultural products. The evaluation was performed by usage of multi-criteria decision making methods, of MAPPAC method. As the result of the analyse is evident the different attitude to foreign culture of citizens in individual EU countries.
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Choi, Jihyoung, Sanjana Mathew, Sabrina Oerter, Antje Appelt-Menzel, Jan Hansmann, and Tobias Schmitz. "Online Measurement System for Dynamic Flow Bioreactors to Study Barrier Integrity of hiPSC-Based Blood–Brain Barrier In Vitro Models." Bioengineering 9, no. 1 (January 16, 2022): 39. http://dx.doi.org/10.3390/bioengineering9010039.
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Electrochemical impedance spectroscopy (EIS) is a noninvasive, reliable, and efficient method to analyze the barrier integrity of in vitro tissue models. This well-established tool is used most widely to quantify the transendothelial/epithelial resistance (TEER) of Transwell-based models cultured under static conditions. However, dynamic culture in bioreactors can achieve advanced cell culture conditions that mimic a more tissue-specific environment and stimulation. This requires the development of culture systems that also allow for the assessment of barrier integrity under dynamic conditions. Here, we present a bioreactor system that is capable of the automated, continuous, and non-invasive online monitoring of cellular barrier integrity during dynamic culture. Polydimethylsiloxane (PDMS) casting and 3D printing were used for the fabrication of the bioreactors. Additionally, attachable electrodes based on titanium nitride (TiN)-coated steel tubes were developed to perform EIS measurements. In order to test the monitored bioreactor system, blood–brain barrier (BBB) in vitro models derived from human-induced pluripotent stem cells (hiPSC) were cultured for up to 7 days. We applied equivalent electrical circuit fitting to quantify the electrical parameters of the cell layer and observed that TEER gradually decreased over time from 2513 Ω·cm2 to 285 Ω·cm2, as also specified in the static control culture. Our versatile system offers the possibility to be used for various dynamic tissue cultures that require a non-invasive monitoring system for barrier integrity.
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Bang, Ja-Yeon. "Understanding and Utilizing Local Culture Based on Seogwipo’s ‘Noji Culture’." Society for Jeju Studies 59 (February 28, 2023): 93–115. http://dx.doi.org/10.47520/jjs.2023.59.93.
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Local culture is composed of various components, and among them, ‘Noji’ is a concept that has been emerging recently. In Jeju, the expression ‘Noji’ is used in ‘noji tangerine’ and ‘noji soju’, which the term contains meaning of unprocessed naturalness.
The subject of this study was selected as the original downtown area of Seogwipo because Seogwipo City was selected as a legal cultural city by the government and presented ‘Noji Culture’ as a brand representing the region.
The purpose of this study is to present the direction of local culture based on ‘Noji Culture’ by discovering open ground cultural resources of the original downtown of Seogwipo City, and to study values and utilization plans. In the process, we want to contribute to forming the identity of the local culture by rediscovering the value of the open ground.
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Gago-Rodríguez, Susana, and Tom Vikson. "Survey-based Evidence on Positive Synergies between Adaptive Culture and a Balanced Scorecard." Revista de Contabilidad 26, no. 1 (January 1, 2023): 111–23. http://dx.doi.org/10.6018/rcsar.418191.
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Prior management accounting research has addressed how organizational culture may substitute or complement the use of decision-making and control of management accounting systems (MASs). However, this paper argues that not all organizational cultures and MASs are equal. Their compatibility determines whether their usage together creates any positive or negative synergies. This paper follows a 2017 survey-based methodology with 102 managers of different nationalities. Collected responses indicate the role that culture plays in their usage of the balanced scorecard (BSC) and their consciousness concerning Key Performance Indicators (KPIs). Remarkably, our survey of managers reveals that adaptive cultures create positive synergies with the BSC and, consequently, positive judgments on the KPI scale. Las investigaciones anteriores sobre contabilidad de gestión han abordado cómo la cultura organizativa puede sustituir o complementar el uso de la toma de decisiones y el control de los sistemas de contabilidad de gestión (SCG). Sin embargo, este artículo sostiene que no todas las culturas organizativas y los SCG son iguales. Su compatibilidad determina si su uso conjunto crea sinergias positivas o negativas. Este trabajo sigue una metodología basada en una encuesta realizada en 2017 a 102 directivos de diferentes nacionalidades. Las respuestas recogidas indican el papel que desempeña la cultura en el uso del cuadro de mando integral (CMI) y su conciencia sobre los indicadores clave de rendimiento (ICR). De forma notable, nuestra encuesta a los directivos revela que las culturas adaptativas crean sinergias positivas con el CMI y, en consecuencia, juicios positivos en la escala de los ICR.
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Liang, Lin, and Shasha Wang. "The Generation and Realization of Dunhuang Cultural Values from the Perspective of Crosscultural Communication: Based on Spanish and Chinese Culture." Journal of Environmental and Public Health 2022 (October 3, 2022): 1–11. http://dx.doi.org/10.1155/2022/5382213.
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Dunhuang Feitian is a complex of Indian culture, Buddhist culture, Western culture, and Central Plains culture. It has been loved by artists since ancient times and is an important embodiment of the essence of grotto art. Dunhuang culture is an important cultural heritage used to build Chinese cultural identity. With the development of science and technology and the progress of society, the exchanges between cultures are increasingly deepened. These exchanges are not only reflected in language, economy, politics, film, and technology but also in science and technology. From a macroperspective, culture includes all products, and products are also an intuitive manifestation of culture. How to continue culture and consider the customs and taboos of the outside world while continuing retains the essence of culture to the greatest extent and integrates local characteristics, so that foreign culture can be integrated into it. Being well accepted by local people will become an important proposition for crosscultural communication and value expression. Therefore, based on cognitive thinking and Kansei engineering theory, this research explores and analyzes product design methods that integrate Spanish and Chinese cultures, hereinafter referred to as “Western-Chinese” cultures. Analyze the two cultures separately: from a certain culture, after the image investigation, extract the target image; from another culture, get the modeling design factors and then realize the product image modeling design of crosscultural Dunhuang integration and the Dunhuang culture. Value generation and realization reasonably recognize the connection between the two cultures, form a crosscultural integration design theory, and provide new ideas for product innovation design.
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Lysgård, Hans Kjetil. "The definition of culture in culture-based urban development strategies: antagonisms in the construction of a culture-based development discourse." International Journal of Cultural Policy 19, no. 2 (March 2013): 182–200. http://dx.doi.org/10.1080/10286632.2011.647005.
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He, Xiaohua, and Shihua Tian. "Analysis of the Communication Method of National Traditional Sports Culture Based on Deep Learning." Scientific Programming 2022 (April 14, 2022): 1–8. http://dx.doi.org/10.1155/2022/9697014.
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Like the Chinese nation, China's national traditional sports culture has a long history and is a treasure of Chinese national culture, reflecting the life, customs, wisdom, and pursuit of the people of all ethnic groups in China. The dissemination of national traditional sports culture is not only a necessary method for our country's cultural export but also a necessary means to maintain the diversity of world sports culture. However, there are still serious deficiencies in the dissemination of national traditional sports culture in our country, and some traditional national sports cultures are not even known to the public. Starting from the two communication methods of sports events and network communication, this paper studies the three mature national traditional sports cultures of martial arts (Wushu), Go, and Chinese chess and proposes a quantitative method for communication methods and communication effects. We further analyze the role of national traditional sports culture in cultural communication.
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Gassl, Vincent, Merel R. Aberle, Bas Boonen, Rianne D. W. Vaes, Steven W. M. Olde Damink, and Sander S. Rensen. "Chemosensitivity of 3D Pancreatic Cancer Organoids Is Not Affected by Transformation to 2D Culture or Switch to Physiological Culture Medium." Cancers 14, no. 22 (November 16, 2022): 5617. http://dx.doi.org/10.3390/cancers14225617.
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Organoids are increasingly used to investigate patient-specific drug responsiveness, but organoid culture is complex and expensive, and carried out in rich, non-physiological media. We investigated reproducibility of drug-responsiveness of primary cell cultures in 2D versus 3D and in conventional versus physiological cell culture medium. 3D pancreatic ductal adenocarcinoma organoid cultures PANCO09b and PANCO11b were converted to primary cell cultures growing in 2D. Transformed 2D cultures were grown in physiological Plasmax medium or Advanced-DMEM/F12. Sensitivity towards gemcitabine, paclitaxel, SN-38, 5-fluorouacil, and oxaliplatin was investigated by cell viability assays. Growth rates of corresponding 2D and 3D cultures were comparable. PANCO09b had a shorter doubling time in physiological media. Chemosensitivity of PANCO09b and PANCO11b grown in 2D or 3D was similar, except for SN-38, to which PANCO11b cultured in 3D was more sensitive (2D: 8.2 ×10−3 ± 2.3 ×10−3 vs. 3D: 1.1 ×10−3 ± 0.6 ×10−3, p = 0.027). PANCO09b and PANCO11b showed no major differences in chemosensitivity when cultured in physiological compared to conventional media, although PANCO11b was more sensitive to SN-38 in physiological media (9.8 × 10−3 ± 0.7 × 10−3 vs. 5.2 × 10−3 ± 1.8 × 10−3, p = 0.015). Collectively, these data indicate that the chemosensitivity of organoids is not affected by culture medium composition or culture dimensions. This implies that organoid-based drug screens can be simplified to become more cost-effective.
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Saeroji, Amad, Tomi Agfianto, Deria Adi Wijaya, Joko Sadoso Priyo, and M. Marimin. "THE IMAGE OF KAMPUNG BALUWARTI AS A JAVANESE CULTURAL EDU-TOURISM DESTINATION BASED ON THE CULTURE OF SURAKARTA SUNANATE PALACE." Jurnal Pengembangan Kota 8, no. 2 (December 3, 2020): 142–50. http://dx.doi.org/10.14710/jpk.8.2.142-150.
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Kampung Baluwarti is a cultural edu-tourism destination in Surakarta City, Central Java, Indonesia. Systematically, this study aims to: 1) identify the characteristics of the tourism products of Kampung Baluwarti; 2) study the development strategies of the image of Kampung Baluwarti as a cultural edu-tourism destination in Surakarta and formulate the cultural education tour packages that are tailored to existing potentials, environment, and regional planning. The research applied a descriptive-qualitative method with PASOLP (Product Analysis of Outdoor Leisure Planning Sequence) which offers a creative, pragmatic, and operational approach to modern tourism area planning. In this case, the research conducted two PASOLP stages: defining the object and attraction and providing recommendations on the needs and priorities. The data collection technique was conducted by collecting the necessary information through interviews with key informants. Field observation carried out the existing condition. Besides, a literature study was used to dig up literary information related to Kampung Baluwarti that was not obtained in interviews and observation. Besides, a literature study was used to explore literary information related to Kampung Baluwarti that was not obtained in interviews and observation. The results show that the tourism products, in this case, have multiple roles: not only for conservation and preservation purposes but also for improving the economy of the local people living in the area. Development strategies that are tailored to product characteristics, local community conditions, and planning are formulated with Javanese traditional tour packages that can form the image of Kampung Baluwarti as a cultural edu-tourism destination in Surakarta. Therefore, cultural edu-tourism destination carries the appropriate concept in developing tourism strategies based on the Javanese culture of Surakarta Sunanate Palace.
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Baudrexler, Tobias, Tobias Boeselt, Lin Li, Sophia Bohlscheid, Ursel Boas, Christoph Schmid, Andreas Rank, Jörg Schmohl, Rembert Koczulla, and Helga Maria Schmetzer. "Volatile Phases Derived from Serum, DC, or MLC Culture Supernatants to Deduce a VOC-Based Diagnostic Profiling Strategy for Leukemic Diseases." Biomolecules 13, no. 6 (June 14, 2023): 989. http://dx.doi.org/10.3390/biom13060989.
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Volatile organic compounds (VOCs) reflect the metabolism in healthy and pathological conditions, and can be collected easily in a noninvasive manner. They are directly measured using electronical nose (eNose), and may qualify as a systemic tool to monitor biomarkers related to disease. Myeloid leukemic blasts can be transformed into leukemia-derived dendritic cells (DCleu) able to improve (anti-leukemic) immune responses. To profile immunological changes in healthy and acute myeloid leukemic (AML) patients’ ex vivo cell cultures, we correlated the cell biological data with the profiles of cell culture supernatant-derived VOCs. DC/DCleu from leukemic or healthy whole blood (WB) were generated without (Control) or with immunomodulatory Kit M (Granulocyte macrophage-colony-stimulating-factor (GM-CSF) + prostaglandin E1 (PGE1)) in dendritic cell cultures (DC culture). Kit-pretreated/not pretreated WB was used to stimulate T cell-enriched immunoreactive cells in mixed lymphocyte cultures (MLC culture). Leukemia-specific adaptive and innate immune cells were detected with a degranulation assay (Deg) and an intracellular cytokine assay (InCyt). Anti-leukemic cytotoxicity was explored with a cytotoxicity fluorolysis assay (CTX). VOCs collected from serum or DC- and MLC culture supernatants (with vs. without Kit M pretreatment and before vs. after culture) were measured using eNose. Compared to the Control (without treatment), Kit M-pretreated leukemic and healthy WB gave rise to higher frequencies of mature (leukemia-derived) DC subtypes of activated and (memory) T cells after MLC. Moreover, antigen (leukemia)-specific cells of several lines (innate and adaptive immunity cells) were induced, giving rise to blast-lysing cells. The eNose could significantly distinguish between healthy and leukemic patients’ serum, DC and MLC culture supernatant-derived volatile phases and could significantly separate several supernatant (with vs. without Kit M treatment, cultured vs. uncultured)-derived VOCs within subgroups (healthy DC or leukemic DC, or healthy MLC or leukemic MLC supernatants). Interestingly, the eNose could indicate a Kit M- and culture-associated effect. The eNose may be a prospective option for the deduction of a VOC-based profiling strategy using serum or cell culture supernatants and could be a useful diagnostic tool to recognize or qualify AML disease.
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Magee, Dashia, and A. J. Meier. "Science Education and Culture: Inquiry-Based Learning." Journal of Intercultural Communication 11, no. 3 (November 10, 2011): 1–14. http://dx.doi.org/10.36923/jicc.v11i3.538.
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At a time when inquiry-based science education is finding increased acceptance, US classrooms are exhibiting a significant increase in diversity. This necessitates attention to the compatibility between the culture of inquiry teaching and the broad range of cultures that form students’ backgrounds. Although some research has considered students’ cultural backgrounds and the roles these might play in the effectiveness of an inquiry approach, none has focused on the specific characteristics of an inquiry approach that might constitute cultural "biases" for a broad range of students with a concomitant broad range of cultural values and beliefs. This article seeks to identify characteristics of an inquiry approach, compare these to the potential cultures students may bring with them to the classroom, using cultural orientations as a heuristic, and suggest construals of inquiry that could better address the needs of a greater number of learners.
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Frias, Ana, Christopher D. Porada, Kirsten B. Crapnell, Joaquim M. S. Cabral, Esmail D. Zanjani, and Graca Almeida-Porada. "Ex-Vivo Generation and Expansion of Both Lymphoid and Myeloid Lineages from Human Cord Blood (CB) HSC Using a Serum-Free Human Mesenchymal Stem Cell Based Culture System." Blood 104, no. 11 (November 16, 2004): 2888. http://dx.doi.org/10.1182/blood.v104.11.2888.2888.
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Abstract The in vitro culture of a hematopoietic stem cell (HSC) graft with either media containing animal-derived components or a feeder layer with ill-defined pathogenic potential such as xenogeneic cell lines or cells modified by viral transformation poses risks that concern scientists and regulatory agencies. In the present studies, we avoided these risks by evaluating the ability of a human stromal-based serum free culture system (hu-ST) to support the ex-vivo expansion/maintenance of human CB HSC. CB CD34+ enriched cells were cultured in serum free medium in the presence of hu-ST with SCF, bFGF, LIF and Flt-3, and the cultures were analyzed for expansion, phenotype and clonogenic ability. We have previously reported the ability of this culture system to allow the successful expansion/maintenance of HSC along the myeloid pathway. In the present study, we investigated whether we could further develop this culture system to simultaneously expand myelopoiesis and lymphopoiesis in vitro. To this end, cord blood CD34+ cells were cultured for a total of 28 days and analyzed every 3 days for expansion and phenotype. There was a progressive increase in CD34 cell number with time in culture. The differentiative profile was primarily shifted towards the myeloid lineage with the presence of CD33, CD15, and CD14. However, a significant number of CD7+ cells were also generated. At week 2 of culture, we observed that 30% of the cells in the culture were CD7 positive. These CD7+CD2-CD3-CD5-CD56-CD16-CD34- cells were then sorted and either plated on top of new irradiated hu-ST layers in the presence of SCF, FLT-3, IL-7, IL-2, and IL-15, or cultured with IL-4, GM-CSF, and FLT-3 in the absence of stroma. Both of these cultures were maintained for an additional 2 weeks. In both sets of cultures, further expansion in the total cell number occurred with the time in culture, and by the end of the week 2, we observed that 25.3±4.18% of the cells had become CD56+ CD3-, a phenotype consistent with that of NK cells. Furthermore, cytotoxicity assays were performed and showed cytotoxic activity that increased in an E:T ratio-dependent fashion. 38.6% of the CD7+ cells grown in the presence of IL-4, GM-CSF, and FLT-3 became CD123+CD11c-, a phenotype characteristic of nonactivated dendritic cells, while 7.3–12.1% adopted an activitated dendritic cell phenotype CD83+CD1a+. In summary, we developed an in vitro culture system that reproducibly allows the effective ex vivo expansion of human cord blood HSCs while maintaining the capability of generating both myeloid and lymphoid hematopoiesis in vitro.
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Panwar, Amit, Prativa Das, and Lay Poh Tan. "3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering." Bioengineering 8, no. 11 (November 14, 2021): 185. http://dx.doi.org/10.3390/bioengineering8110185.
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Liver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better mimic the in vivo microenvironment, and thus help in maintaining metabolic activity, phenotypic properties, and longevity of the in vitro cultures. Again, the culture method and type of cell population are also recognized as important parameters for functional maintenance of primary hepatocytes. Hepatic organoids formed by self-assembly of hepatic cells are microtissues, and were able to show long-term in vitro maintenance of hepato-specific characteristics. Thus, hepatic organoids were recognized as an effective tool for screening potential cures and modeling liver diseases effectively. The current review summarizes the importance of 3D hepatic organoid culture over other conventional 2D and 3D culture models and its applicability in Liver tissue engineering.
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Xiang, Fu, Long J. Yu, Wu Chen, and Zhi Liu. "Effect of Cell Culture on 18S rRNA Gene Sequences in the Cultural Course of Taxus chinensis Cells." Zeitschrift für Naturforschung C 63, no. 1-2 (February 1, 2008): 127–32. http://dx.doi.org/10.1515/znc-2008-1-223.
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Cell culture is an effective technology for taxol production. This paper discusses the effect of Taxus cell cultures on the 18S rRNA gene sequences based on the phylogenetic analysis of cultured T. chinensis cells and related species. The phylogenetic tree is reconstructed using the maximum parsimony method and the relative rate test to test the hypothesis of a molecular clock. The phylogenetic analysis indicates that cell culture changes the phylogenetic position of cultured T. chinensis cells. More than that, the 18S rRNA gene of cultured T. chinensis cells has a faster rate of substitution than that of T. chinensis. With T. media as reference, the divergence time of the cultured T. chinensis cells is 7 Ma (million years) more than that of the T. chinensis cells based on the 18S rRNA gene sequences.
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Finoli, Anthony, Eva Schmelzer, Patrick Over, Ian Nettleship, and Joerg C. Gerlach. "Open-Porous Hydroxyapatite Scaffolds for Three-Dimensional Culture of Human Adult Liver Cells." BioMed Research International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/6040146.
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Liver cell culture within three-dimensional structures provides an improved culture system for various applications in basic research, pharmacological screening, and implantable or extracorporeal liver support. Biodegradable calcium-based scaffolds in such systems could enhance liver cell functionality by providing endothelial and hepatic cell support through locally elevated calcium levels, increased surface area for cell attachment, and allowing three-dimensional tissue restructuring. Open-porous hydroxyapatite scaffolds were fabricated and seeded with primary adult human liver cells, which were embedded within or without gels of extracellular matrix protein collagen-1 or hyaluronan. Metabolic functions were assessed after 5, 15, and 28 days. Longer-term cultures exhibited highest cell numbers and liver specific gene expression when cultured on hydroxyapatite scaffolds in collagen-1. Endothelial gene expression was induced in cells cultured on scaffolds without extracellular matrix proteins. Hydroxyapatite induced gene expression for cytokeratin-19 when cells were cultured in collagen-1 gel while culture in hyaluronan increased cytokeratin-19 gene expression independent of the use of scaffold in long-term culture. The implementation of hydroxyapatite composites with extracellular matrices affected liver cell cultures and cell differentiation depending on the type of matrix protein and the presence of a scaffold. The hydroxyapatite scaffolds enable scale-up of hepatic three-dimensional culture models for regenerative medicine applications.
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Jose, Gils, K. T. Shalumon, and Jyh-Ping Chen. "Natural Polymers Based Hydrogels for Cell Culture Applications." Current Medicinal Chemistry 27, no. 16 (June 4, 2020): 2734–76. http://dx.doi.org/10.2174/0929867326666190903113004.
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It is well known that the extracellular matrix (ECM) plays a vital role in the growth, survival and differentiation of cells. Though two-dimensional (2D) materials are generally used as substrates for the standard in vitro experiments, their mechanical, structural, and compositional characteristics can alter cell functions drastically. Many scientists reported that cells behave more natively when cultured in three-dimensional (3D) environments than on 2D substrates, due to the more in vivo-like 3D cell culture environment that can better mimic the biochemical and mechanical properties of the ECM. In this regard, water-swollen network polymer-based materials called hydrogels are highly attractive for developing 3D ECM analogs due to their biocompatibility and hydrophilicity. Since hydrogels can be tuned and altered systematically, these materials can function actively in a defined culture medium to support long-term self-renewal of various cells. The physico-chemical and biological properties of the materials used for developing hydrogel should be tunable in accordance with culture needs. Various types of hydrogels derived either from natural or synthetic origins are currently being used for cell culture applications. In this review, we present an overview of various hydrogels based on natural polymers that can be used for cell culture, irrespective of types of applications. We also explain how each hydrogel is made, its source, pros and cons in biological applications with a special focus on regenerative engineering.
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Yoshimura, K., A. Hagiwara, T. Yoshimatsu, and C. Kitajima. "Culture technology of marine rotifers and the implications for intensive culture of marine fish in Japan." Marine and Freshwater Research 47, no. 2 (1996): 217. http://dx.doi.org/10.1071/mf9960217.
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In order to expand the practice of rearing marine finfish larvae, it is necessary to increase and stabilize the production of live feed. Latest researches on rotifer culture technology in Japan have aimed at these objectives. This paper summarizes the progress on the technology of high-density rotifer mass cultures. The high-density culture of the marine rotifer Brachionus rotundiformis (so-called S-type) enables production that is at least 100 times more efficient than that by the conventional method, with less cost for production. The technology is based on the use of highly concentrated algal biomass as food for rotifers. Rotifers are mass-cultured at 10000-30000 individuals mL-1 by feeding them exclusively on condensed freshwater Chlorella. Cultures are generally supplied with constant O2; pH is adjusted to 7 by addition of HCI to avoid an increase of free ammonia in the culture system. The excess suspended organic matter can be efficiently removed by a nylon filtration mat.
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van Riet, Sander, Annemarie van Schadewijk, P. Padmini S. J. Khedoe, Ronald W. A. L. Limpens, Montserrat Bárcena, Jan Stolk, Pieter S. Hiemstra, and Anne M. van der Does. "Organoid-based expansion of patient-derived primary alveolar type 2 cells for establishment of alveolus epithelial Lung-Chip cultures." American Journal of Physiology-Lung Cellular and Molecular Physiology 322, no. 4 (April 1, 2022): L526—L538. http://dx.doi.org/10.1152/ajplung.00153.2021.
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Development of effective treatment strategies for lung tissue destruction as seen in emphysema would greatly benefit from representative human in vitro models of the alveolar compartment. Studying how cellular cross talk and/or (altered) biomechanical cues affect alveolar epithelial function could provide new insight for tissue repair strategies. Preclinical models of the alveolus ideally combine human primary patient-derived lung cells with advanced cell culture applications such as breathing-related stretch, to reliably represent the alveolar microenvironment. To test the feasibility of such a model, we isolated primary alveolar type 2 cells (AEC2s) from patient-derived lung tissues including those from patients with severe emphysema, using magnetic bead-based selection of cells expressing the AEC2 marker HTII-280. We obtained pure alveolar feeder-free organoid cultures using a minimally modified commercial medium. This was confirmed by known AEC2 markers as well as by detection of lamellar bodies using electron microscopy. Following (organoid-based) expansion, cells were seeded on both cell culture inserts and the Chip-S1 Organ-Chip that has a flexible polydimethylsiloxane (PDMS) membrane enabling the application of dynamic stretch. AEC2s cultured for 7 days on inserts or the chip maintained expression of HTII-280, prosurfactant protein C (SP-C), SP-A and SP-B, and zonula occludens-1 (ZO-1) also in the presence of stretch. AEC2s cultured on the chip showed lower expression levels of epithelial-mesenchymal transition-related vimentin expression compared with static cultures on inserts. The combination of a straightforward culture method of patient-derived AEC2s and their application in microfluidic chip cultures supports successful development of more representative human preclinical models of the (diseased) alveolar compartment.
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Jarrahy, Reza, Weibiao Huang, George H. Rudkin, Jane M. Lee, Kenji Ishida, Micah D. Berry, Modar Sukkarieh, Benjamin M. Wu, Dean T. Yamaguchi, and Timothy A. Miller. "Osteogenic differentiation is inhibited and angiogenic expression is enhanced in MC3T3-E1 cells cultured on three-dimensional scaffolds." American Journal of Physiology-Cell Physiology 289, no. 2 (August 2005): C408—C414. http://dx.doi.org/10.1152/ajpcell.00196.2004.
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Osteogenic differentiation of osteoprogenitor cells in three-dimensional (3D) in vitro culture remains poorly understood. Using quantitative real-time RT-PCR techniques, we examined mRNA expression of alkaline phosphatase, osteocalcin, and vascular endothelial growth factor (VEGF) in murine preosteoblastic MC3T3-E1 cells cultured for 48 h and 14 days on conventional two-dimensional (2D) poly(l-lactide-co-glycolide) (PLGA) films and 3D PLGA scaffolds. Differences in VEGF secretion and function between 2D and 3D culture systems were examined using Western blots and an in vitro Matrigel-based angiogenesis assay. Expression of both alkaline phosphatase and osteocalcin in cells cultured on 3D scaffolds was significantly downregulated relative to 2D controls in 48 h and 14 day cultures. In contrast, elevated levels of VEGF expression in 3D culture were noted at every time point in short- and long-term culture. VEGF protein secretion in 3D cultures was triple the amount of secretion observed in 2D controls. Conditioned medium from 3D cultures induced an enhanced level of angiogenic activity, as evidenced by increases in branch points observed in in vitro angiogenesis assays. These results collectively indicate that MC3T3-E1 cells commit to osteogenic differentiation at a slower rate when cultured on 3D PLGA scaffolds and that VEGF is preferentially expressed by these cells when they are cultured in three dimensions.
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Çakir, Ismail. "The Efficacy of Culture Based Syllabus in Teaching the Target Culture." Anthropologist 21, no. 1-2 (July 2015): 219–30. http://dx.doi.org/10.1080/09720073.2015.11891811.
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Chen, Siyu, Bin Meng, Na Liu, Zhenyu Qi, Jian Liu, and Juan Wang. "Cultural Perception of the Historical and Cultural Blocks of Beijing Based on Weibo Photos." Land 11, no. 4 (March 29, 2022): 495. http://dx.doi.org/10.3390/land11040495.
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Historic blocks are steeped in the history and culture of a city, reflecting the characteristics of the landscape during historical periods; they are of great significance to the preservation of the historical memory of the city. However, existing research generally lacks integration with big data, and research perspectives are mostly from the conservation planning of historic blocks, the evaluation mechanisms of blocks, and the development of block tourism resources; thus, the public perception is lacking. This study involved 28 historical and cultural blocks in Beijing, the capital of China, and constructed a system of cultural perception symbols based on the cultural connotations of the capital. On this basis, photo recognition was conducted on Weibo photo data collected by relying on the photo segmentation method, and the intrinsic factors affecting the cultural perception of the historical and cultural blocks were explored from the perspective of public perception (local residents and tourists). The results show that: (1) the capital culture of Beijing can be roughly divided into five categories: ancient capital culture, red culture, Beijing-style culture, innovation culture, and other types of culture, based on the photo recognition method; (2) from the perspective of public perception, the degree of perception of Beijing culture and innovation culture is generally higher, while the degree of perception of red culture and ancient capital culture is lower; (3) the 28 historic and cultural blocks of Beijing’s old city are mainly dominated by one or more cultures, and there are no historic and cultural blocks with a balance of five cultures, reflecting the relative lack of cultural diversity within the blocks; (4) the local cultural identity of Beijing is prominent and dominant in the public mind. However, local residents have a relatively high perception of innovative culture, whereas tourists have a higher perception of ancient capital culture and red culture. In this study, photo recognition technology was introduced to study the cultural perception of historical and cultural blocks to provide new ideas and methods for the study of historical and cultural blocks.
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Guo, Xiaoyue, and Jingjing Feng. "Traditional Cultural Short Video Publicity System Based on Data Mining and Digital Image Technology." Advances in Multimedia 2021 (December 11, 2021): 1–8. http://dx.doi.org/10.1155/2021/6335966.
Full textAbstract:
With the continuous development of the social economy, more and more attention has been paid to traditional cultures, such as Chinese-style clothing, decoration, and furniture, and the publicity of traditional culture is also diverse. However, it should be noted that the rise of short videos has promoted the diversification of traditional culture publicity methods. In this paper, data mining technology and digital technology are introduced. By sorting out the content, publicity strategy, and emphasis of traditional culture, and through correlation analysis, the audience of traditional culture has been wider, and the content has been transmitted more, aiming to make more Chinese people love traditional culture and promote traditional culture. The simulation results show that data mining and digital technology are effective and can effectively promote the propaganda of short videos of traditional culture.
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Huang, Xiaosong, L. Jeanne Pierce, Paul A. Cobine, Dennis R. Winge, and Gerald J. Spangrude. "Copper Modulates the Differentiation of Mouse Hematopoietic Progenitor Cells in Culture." Cell Transplantation 18, no. 8 (August 2009): 887–97. http://dx.doi.org/10.3727/096368909x471152.
Full textAbstract:
Copper chelation has been shown to favor the expansion of human hematopoietic stem/progenitor cells in vitro. To further understand the effects of copper modulation on defined subsets of stem cells versus progenitor cells, we extended the studies in a mouse system. We isolated mouse hematopoietic stem cells (HSCs) or hematopoietic progenitor cells (HPCs) and cultured them with or without the copper chelator tetraethylenepentamine (TEPA) or CuCl2. Cytokine-stimulated HPC cultures treated with TEPA for 7 days generated about two to three times more total and erythroid colony-forming cells (CFCs) compared to control cultures. In contrast, CuCl2 treatment decreased the CFC numbers. Similar results were seen with HSC after 14, but not 7, days of culture. Transplant studies showed that HPCs cultured for 7 days in TEPA had about twofold higher short-term erythroid repopulation potential compared to control cultures, while CuCl2 decreased the erythroid potential of cultured HPCs compared to control cultures. HSCs cultured with TEPA for 7 days did not exhibit significantly higher repopulation potential in either leukocyte or erythrocyte lineages compared to control cultures in short-term or long-term assays. Based on JC-1 staining, the mitochondrial membrane potential of HPCs cultured with TEPA was lower relative to control cultures. Our data suggest that decreasing the cellular copper content with TEPA results in preferential expansion or maintenance of HPC that are biased for erythroid differentiation in vivo, but does not enhance the maintenance of HSC activity in culture.
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Jiang, Rui, and Chuanbin Jiang. "Difficulties and Suggestions in TCM Translation based on Acculturation Theory." Journal of Education and Educational Research 3, no. 3 (June 1, 2023): 124–33. http://dx.doi.org/10.54097/jeer.v3i3.9639.
Full textAbstract:
This study analyzes the main problems and obstacles in TCM translation, including cultural and linguistic barriers, shortage of professional translators, and lack of unified standards. To clear these obstacles, based on the theory of acculturation, this study analyzes the corresponding methods of TCM translation from four aspects: cultural separation, cultural marginalization, cultural integration, and cultural assimilation, starting from the target language group's identity and tendency toward its original culture and the mainstream culture (TCM). In doing so, the differences between Chinese and Western cultures may be balanced by TCM translation and the international spread of TCM culture may be promoted.
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Qassem, Mutahar, and Vijayasarathi Gurindapalli. "Culture-based text analysis in translation." Linguistik Online 95, no. 2 (June 5, 2019): 91–107. http://dx.doi.org/10.13092/lo.95.5517.
Full textAbstract:
Comprehension can be described as the ability to construct the meaning of a text, spoken or written. Such ability requires a complex mix of linguistic and world knowledge acquired through interactions with the global environment, transcending language, and cultural translation barriers. In addition, translation schools propose various models to address the difficulties inherent in comprehending the source text (ST) by using analysis from the linguistic, sociolinguistic, or cognitive perspectives. On the basis of translation schools’ models of text analysis, this study similarly attempts to provide a corpus-based analysis of culture-based texts from authentic sources (i. e. British online news). Results corroborate that the comprehension of culture- based texts can be attained through textual and lexical analyses. Further, the cultural, linguistic, sociolinguistic, and cognitive perspectives of translation can be employed to create a positive interaction between the internal and external components of a ST text on the one hand, and a translator on the other hand. Drawing on the multimodality of text analysis, this study attempts to offer a practical model for culture-based text analysis that can be applied at a translation class or course level.