Academic literature on the topic 'Culture affinity'
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Journal articles on the topic "Culture affinity"
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Garle, Michael J., and Jeffrey R. Fry. "A Comparison of Hepatic Enzyme Activities and their Modulation by Dexamethazone in Freshly Isolated and Cultured Hepatocytes and in the Differentiated Hepatoma Cell Line, 2sFou." Alternatives to Laboratory Animals 24, no. 1 (January 1996): 31–37. http://dx.doi.org/10.1177/026119299602400106.
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Rodent hepatocytes are mitotically inhibited and lose hepatospecific functions over time in culture. In contrast, some differentiated hepatoma cell lines express stable hepatospecific functions in culture, but at much lower levels than those initially found in primary hepatocytes. A number of hepatospecific functions were measured in freshly isolated and cultured rat hepatocytes; these were compared to activities found in the differentiated Reuber hepatoma cell line, 2sFou. The effects of dexamethazone on these activities were also investigated, since dexamethazone is reported to enhance the expression of organotypic functions. The P450-related activities (ethoxyresorufin-O-deethylase activity and pentoxyresorufin-O-depenty-lase activity) and glucose-6-phosphatase activity declined in hepatocytes with increasing time in culture. The same activities in 2sFou cells were similar to those in hepatocytes which had been cultured for 72 hours. Tyrosine amino transferase (TAT) activity declined in hepatocyte cultures with time, but dexamethazone (1μM) restored activity up to freshly isolated cell values. TAT activity in hepatoma cells exceeded that in hepatocytes and was highly inducible by dexamethazone. γ-Glutamyl transpeptidase activity increased with culture time in hepatocytes and was also highly expressed in 2sFou cells. In hepatocytes, the activity of a high affinity alcohol dehydrogenase (ADH) declined with time in culture. In 2sFou cells, there was evidence of a low affinity (extra-hepatic or fetal) form of ADH, which was not evident in cultured hepatocytes.
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Smith, John. "The trinitarian dance with culture: Trinity as the missiological optic for understanding culture." Missiology: An International Review 48, no. 2 (January 8, 2020): 154–68. http://dx.doi.org/10.1177/0091829619887386.
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In discussions of imago Dei, we largely confine ourselves to functional and moral affinity to God, that is that we have been made in God’s image with the result that obedience to God amounts to imitating his moral character and behavior. However, we need to add the existential affinity of God’s being to our understanding of God’s image in us, specifically the fact that he is both singular (in essence) and plural (in person). To some degree, people share God’s singular–plural quality and that one–many affinity can give great insight into gospel ministry. Moreover, as with the moral dimension of imago Dei, this singular–plural dimension also was marred by sin so that we, as individuals and as whole cultures, tend to lean toward one extreme or the other, toward singularity (unhealthy individualism) or plurality (monolithic communalism). The gospel of grace addresses both. Therefore, as we prepare to do ministry, we should study our audience to discern the existential lean of its own incarnation of imago Dei, so that we can best speak the gospel to fit audience need.
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Roos, Joseph W., and Martin A. Hjortso. "Control of anEscherichia colimixed culture via affinity binding." Biotechnology and Bioengineering 38, no. 4 (August 5, 1991): 380–88. http://dx.doi.org/10.1002/bit.260380408.
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Suranovic, Steven, and Robert Winthrop. "Trade Liberalization and Culture." Global Economy Journal 14, no. 1 (February 13, 2014): 57–78. http://dx.doi.org/10.1515/gej-2013-0047.
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This paper addresses the effect of international trade on cultural outcomes from both economic and anthropological perspectives. Definitions of culture are informed by anthropology and then incorporated into a standard economic trade models in two distinct ways. In the “cultural affinity from work” model, workers receive a non-pecuniary cultural benefit from work in a particular industry. In the “cultural externality” model, consumers of a product receive utility from other consumer’s consumption of a domestic good. We show that resistance to change due to cultural concerns can reduce the national benefits from trade liberalization. Complete movements to free trade will have a positive national welfare impact in the cultural affinity case, whereas it may lower national welfare in the cultural externality case. We also show that a loss of cultural benefits is more likely to occur when culture is an externality.
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Dickman, K. G., and J. L. Renfro. "Primary culture of flounder renal tubule cells: transepithelial transport." American Journal of Physiology-Renal Physiology 251, no. 3 (September 1, 1986): F424—F432. http://dx.doi.org/10.1152/ajprenal.1986.251.3.f424.
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Renal proximal tubule cells from the winter flounder (Pseudopleuronectes americanus) were maintained in a functionally differentiated state for up to 16 days in primary culture on floating collagen gels. The cells were confluent after 7-8 days in culture, contracted the collagen gels, and exhibited ciliary activity. Electron microscopy indicated that the cultures were composed of continuous sheets of columnar epithelial cells that had established structural polarity. When mounted in Ussing chambers, the cultures exhibited a small mucosa-negative potential difference (0.6 +/- 0.10 mV) and a low transepithelial resistance (23 +/- 2.3 omega X cm2). Short-circuit current averaged 24 microA/cm2. The cultured epithelium was four times more permeable to Na than to Cl and actively secreted sulfate and p-aminohippuric acid and reabsorbed hexoses. Glucose reabsorption was rheogenic and occurred via a high-affinity (Km = 0.16 mM), low-capacity (Vmax = 5 microA/cm2), phlorizin-sensitive transport system. We concluded that the cultured cells express many of the differentiated properties of the intact flounder proximal tubule and thus provide a suitable model system for studying renal transport processes.
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van Dijk, H. P., M. J. Kroos, J. S. Starreveld, H. G. van Eijk, S. P. Tang, D. X. Song, and U. Muller-Eberhard. "Expression of haemopexin receptors by cultured human cytotrophoblast." Biochemical Journal 307, no. 3 (May 1, 1995): 669–72. http://dx.doi.org/10.1042/bj3070669.
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The expression of cell-surface haemopexin (Hx) receptors on human cytotrophoblasts was assessed by using four different Hx species purified from plasma: human Hx isolated by wheatgerm-affinity chromatography, human Hx isolated by haem-agarose-affinity chromatography and rabbit and rat Hx, also isolated by haem-agarose-affinity chromatography. About 3500-7000 high-affinity (Kd 0.34-0.85 nM) receptors per cell were measured by Scatchard-type analysis at 4 degrees C using human (species obtained by both methods) or rabbit 125I-labelled haem-Hx. Measured simultaneously, transferrin receptor number and affinity were 40,000/cell and 0.83 nM respectively. In contrast with transferrin receptors, the number of Hx receptors did not increase during 24 h in cytotrophoblast culture. Rat Hx showed no specific binding to human Hx receptors in cytotrophoblast cultures.
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Eckel, J., G. van Echten, and H. Reinauer. "Adult cardiac myocytes in primary culture: cell characteristics and insulin-receptor interaction." American Journal of Physiology-Heart and Circulatory Physiology 249, no. 2 (August 1, 1985): H212—H221. http://dx.doi.org/10.1152/ajpheart.1985.249.2.h212.
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Calcium-tolerant adult cardiac myocytes were kept in culture under serum-free conditions in the presence of physiological concentrations of insulin. Up to 4 days, 70% of cells retained their in vivo rodshaped morphology without gross structural alterations. During that period a constant ATP-to-ADP ratio was observed with a mean value of 10.6 +/- 0.5 (n = 4). The rate of [14C]phenylalanine incorporation remained unaltered up to 63 h in culture. Insulin binding to cultured cells was found to be time-and temperature-dependent, reversible, and highly specific. Scatchard analysis of equilibrium binding data showed a curvilinear plot with a high-affinity segment yielding an apparent dissociation constant of 4.5 X 10(-10) mol/l and a receptor number of 125,000 sites/cell. Both affinity and receptor number remained unaltered between 18 and 66 h in culture. [14C]phenylalanine incorporation was stimulated by 108% in cardiocytes cultured in the presence of high concentrations of insulin (1.7 X 10(-7) mol/l) for 63 h, when compared with control cells cultured in the absence of insulin. These data demonstrate the retention of structural integrity, insulin receptors, and insulin responsiveness in primary cultured adult cardiac myocytes and provide a useful model for long-term studies on the regulation of insulin action on the heart.
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Sinclair, P. R., W. J. Bement, N. Gorman, H. H. Liem, A. W. Wolkoff, and U. Muller-Eberhard. "Effect of serum proteins on haem uptake and metabolism in primary cultures of liver cells." Biochemical Journal 256, no. 1 (November 15, 1988): 159–65. http://dx.doi.org/10.1042/bj2560159.
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A role of haemopexin in transporting haem to hepatocytes for degradation has been inferred from the high affinity of haemopexin for haem. We have examined this question in primary cultures of chick-embryo and adult rat liver cells. We present here the results of four sets of experiments which indicate that haemopexin retarded haem uptake by hepatocytes in culture. (1) Haem bound to bovine serum albumin is known to repress the activity of delta-aminolaevulinate synthase in chick cultures as indicated by decreased porphyrin accumulation. When haem-albumin was added in the presence of excess purified or freshly secreted chicken haemopexin, no haem-mediated repression of porphyrin production was observed. The haem-mediated repression of porphyrin accumulation was partially prevented when human, but not chicken, albumin was added to cultures. This finding reflected the higher affinity of human albumin for haem compared with that of chicken albumin. (2) Haemopexin inhibited the ability of haem to be incorporated into cytochrome P-450 induced in the chick cultures in the presence of the iron chelator desferrioxamine. (3) The rate of association of [55Fe]haem with cultured rat hepatocytes when [55Fe]haem-haemopexin was added was one-eighth of the rate observed when [55Fe]haem-bovine serum albumin was used as the haem donor. (4) The presence of haemopexin also diminished the catabolism of haem by both rat and chick-embryo liver cell cultures. It is concluded that the uptake and subsequent metabolic effects of haem are inhibited in cultured hepatocytes by proteins such as haemopexin which have a high affinity for haem.
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WEXLER, ERIC M., OKSANA BERKOVICH, and SCOTT NAWY. "Role of the low-affinity NGF receptor (p75) in survival of retinal bipolar cells." Visual Neuroscience 15, no. 2 (February 1998): 211–18. http://dx.doi.org/10.1017/s095252389815201x.
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We have examined the role of neurotrophins in promoting survival of mammalian rod bipolar cells (RBC) in culture. Retinas taken from 8- to 10-day-old Long-Evans rats were dissociated and cultured in media supplemented with either nerve growth factor (NGF), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), or basic fibroblast growth factor (FGF-2). Survival was measured by the number of cells that were immunoreactive for α-, β-, γ-PKC, a bipolar cell-specific marker. Compared to untreated cultures, CNTF had no effect on RBC survival, while NGF and NT-3 increased survival only slightly. BDNF, however, increased survival by approximately 300%. Similar results were obtained with FGF-2. Both nerve growth factor (NGF) and an antibody (anti-REX) which interferes with binding to the 75-kD low-affinity neurotrophin receptor (p75NTR) eliminated BDNF-promoted survival, but had no effect on FGF-2-mediated survival. Interestingly, p75NTR was expressed by retinal glia (Müller cells), but not by the bipolar cells themselves, providing for the possibility that BDNF might induce Müller cells to produce a secondary factor, perhaps FGF-2, which directly rescues RBCs. In support of this hypothesis, an antibody that neutralizes FGF-2 attenuated the trophic effects of BDNF, and dramatically reduced survival in cultures with no added growth factors, indicating that there may be an endogenous source of FGF-2 that promotes survival of RBCs in culture. We suggest that BDNF increases production or release of FGF-2 by binding to p75NTR on Müller cells.
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Wang, Guang Jian, Hye Joo Chung, Jamie Schnuer, Kara Pratt, Anthony C. Zable, Michael P. Kavanaugh, and Paul A. Rosenberg. "High Affinity Glutamate Transport in Rat Cortical Neurons in Culture." Molecular Pharmacology 53, no. 1 (January 1, 1998): 88–96. http://dx.doi.org/10.1124/mol.53.1.88.
Full textDissertations / Theses on the topic "Culture affinity"
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Opitz, Lars [Verfasser]. "Development and characterization of affinity- and pseudo-affinity-based methods for cell culture-derived influenza virus capturing / Lars Opitz." Aachen : Shaker, 2010. http://d-nb.info/1081887109/34.
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Snead, Todd Edwin. "Dichotomous Musical Worlds: Interactions between the Musical Lives of Adolescents and School Music-Learning Culture." Digital Archive @ GSU, 2009. http://digitalarchive.gsu.edu/msit_diss/53.
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This ethnographic study investigated the interactions between the musical lives of adolescents and school music-learning culture in a suburban high school. Participants included two music teachers and seven adolescents. Framed within a symbolic interactionist perspective (Blumer, 1969), data were collected via methods consistent with qualitative inquiry, including an innovative data collection technique utilizing music elicitation interviews with adolescents. Findings emerged from the data via thematic analysis (Grbich, 2007). Findings indicate limited interactions between the musical lives of adolescents and school music-learning culture because participants portrayed and experienced a dichotomy between the musical assumptions and practices inside and outside of school. Interactions occurred when participants engaged in sharing musical capital that overcame segmentation among music learning, out-of-school experience, and elective participation in secondary school music programs. Supporting findings indicate that the school music-learning culture derived from teachers' negotiating between two major influences: 1) their own musical values, which were based on their musical backgrounds and the long-established professional tradition of formal performance emphases in school music programs; and 2) the musical values of their students. Adolescents self-defined their musical lives as largely informal musical activities commonly experienced outside of school. They expressed a wealth of personal musical knowledge and described their affinity for music across four dimensions: 1) expression and feeling, 2) relevance, 3) quality in artistry and craftsmanship, and 4) diversity. Three themes describe how adolescents’ personal relationships with music influenced their beliefs and choices regarding music participation and learning: 1) musical roots: nurturing personal and social connections with music, 2) motivated learning: seeking relevance and challenge, and 3) finding a voice: striving toward musical independence. Findings indicate that music teachers may enhance interactions between adolescents’ musical lives and school music-learning culture by acknowledging students’ musical engagement outside of school, honoring their personal musical knowledge and interests, and making them collaborators in developing music-learning models rooted in their affinity for, and personal relationships with, music.
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Caldera-Noriega, Juan Bautista, and jean_caldera@hotmail com. "A purchaser�s perspective of environmental uncertainty in the international manufacturing products supply chain." RMIT University. Management, 2005. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20060314.162500.
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International companies are increasingly taking advantage of the benefits of globalisation such as continuous improvement in transport and communication technologies that in turn lower transportation costs and decrease logistical difficulties. However, the international environment includes business uncertainty in the form of exchange rate volatility as well as political and economic issues. Moreover, ongoing reduction of trade barriers is reversing the previous tendency to integrate business vertically, shifting international business schemes towards outsourcing all except what the company consider its core competence. Supply chain management is a strategic concept that considers the behavioural and political dimensions of conflict and dependence at the purchaser-supplier interface. Nevertheless, there is substantive ambiguity regarding its actual practice. Furthermore, although international purchasers are increasingly becoming more important, the purchasing si de of international trade remains largely ignored in empirical research. This study focuses on understanding the complexity surrounding the commercial relationship between purchasing executives and foreign suppliers. The aim is to investigate what key factors are associated with uncertainty at the cross-national purchaser-supplier interface. Of interest to this research is how purchasing executives deal with those variables. The literature review addresses the discussions of the constructs as it informs the development of hypotheses investigating patterns arising from their interactions. The result was a theoretically driven model addressing the complexity of the purchaser-supplier interface. A survey was conducted to collect data from the membership of a nation-wide professional body of Australian purchasing executives. The richness of the management reality was approached by having recourse to systems theory and the realism paradigm. The model was tested and discussed using structural equation modelling, the supply chain management literature and the responses and comments of the participating purchasing executives buying from over twenty countries. xxii The results include purchaser�s perceptions regarding five latent variables and their concurrent interactions within the uncertainty surrounding international trade. The factors are culture affinity, the strength of the commercial relationship, quality reliability, ISO 9000 certification and the country of origin effect. Findings indicate that culture affinity is particularly important for purchasing executives and that it is a significant driver of the commercial relationship with foreign suppliers of manufacturing products. Nevertheless, culture affinity does not significantly influence perceptions of quality reliability. In contrast, commitment, cooperation and conflict management have a significant influence when assessing foreign supplier�s quality reliability. Trust, traditionally seen as a social aspect of the commercial relationship, was found structurally embedded in the perceptions of quality reliability together with technical dimensions of quality. The commercial relationship between Australi an purchasing executives and foreign suppliers was found to have a high level of maturity. However, the purchasers showed no orientation towards commercial equity, and cooperation was found to be incomplete, overlooking working on cost optimisation. Further, a level of commercial partnership has not been established. It is suggested that a relational approach in the international supply chain is a necessary, but insufficient, condition to progress to the level of commercial partnerships. The study shows that Australian purchasers� perceptions regarding ISO 9000 are surrounded by controversy. However, the Standard is still considered a credible quality management system tool that enhances supplier�s competitive position. Additionally, the majority of the Australian purchasing companies were not engaged in a low-cost country sourcing strategy. Overall, it can be said that the country of origin effect and ISO 9000 certification did not have a significant influence on quality perceptions within the context of an uncertain international environment. By taking a multivariable and multidisciplinary perspective, this research contributes to a deeper understanding of mature commercial relationships in the international supply chain. The findings provide valuable information that assists organisations to tune or balance the supply management philosophy principles in the uncertainty associated with global trade.
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Tay, Geniesa. "Embracing LOLitics: Popular Culture, Online Political Humor, and Play." Thesis, University of Canterbury. Media and Communication, 2012. http://hdl.handle.net/10092/7091.
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The Internet, and Web 2.0 tools can empower audiences to actively participate in media creation. This allows the production of large quantities of content, both amateur and professional. Online memes, which are extensions of usually citizen-created viral content, are a recent and popular example of this. This thesis examines the participation of ordinary individuals in political culture online through humor creation. It focuses on citizen-made political humor memes as an example of engaged citizen discourse. The memes comprise of photographs of political figures altered either by captions or image editing software, and can be compared to more traditional mediums such as political cartoons, and 'green screens' used in filmmaking. Popular culture is often used as a 'common language' to communicate meanings in these texts. This thesis thus examines the relationship between political and popular culture. It also discusses the value of 'affinity spaces', which actively encourage users to participate in creating and sharing the humorous political texts. Some examples of the political humor memes include: the subversion of Vladimir Putin's power by poking fun at his masculine characteristics through acts similar to fanfiction, celebrating Barack Obama’s love of Star Wars, comparing a candid photograph of John McCain to fictional nonhuman creatures such as zombies using photomanipulation, and the wide variety of immediate responses to Osama bin Laden's death. This thesis argues that much of the idiosyncratic nature of the political humor memes comes from a motivation that lies in non-serious play, though they can potentially offer legitimate political criticism through the myths 'poached' from popular culture.
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Briane, Dominique. "Etude de quelques transitions structurales de l'ADN et de la chromatine en relation avec le cycle cellulaire et la différenciation terminale." Paris 13, 1987. http://www.theses.fr/1987PA132017.
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Les changements affectant la structure de la chromatine ont été etudiés dans le foie de rat en régéneration après hépatectomie partielle. Fractionnement de la chromatine par précipitation magnesienne et chromatographie d'affinité met en évidence une augmentation de la fraction de condensée de la chromatine. Modifications de la chromatine ont été étudiées au cours du cycle céllulaire physarum polycephalum, les cinétiques de digestion de la chromatine par la dnase i ont permis de distinguer deux types de structure (de type chromosomique relativement resistante, et une autre plus accessible a l'enzyme)
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Vignal, Marie-Noëlle. "Alain-Fournier : culture et création, influences et affinités." Clermont-Ferrand 2, 1994. http://www.theses.fr/1994CLF20066.
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Ce travail vise à dépasser le mythe forgé par la critique hagiographique autour de l'auteur du "Grand Meaulnes". Les échanges épistolaires d'Alain-Fournier avec J. Rivière constituent un fil d'ariane indispensable pour retracer ses années de formation fertiles en rencontres livresques. Parmi ces écrivainss lus souvent avec un enthousiasme juvénile, nous distingons la position centrale occupée par Claudel, Gide, dans lesquels nous avons reconnu les maîtres-penseurs ayant un rôle certain sur sa démarche créatrice et son esthétique romanesque. Nous nous interessons ensuite à la culture artistique de l'auteur tout particulièrement aux préraphaëlites connus lors de son séjour en Angleterre, aux peintres symbolistes, Carrière, Redon, auxquels le roman a peut-être emprunté son atmosphère de rêve. Maurice Denis, peintre du renouveau chrétien d'avant-guerre, réveilla sans doute en lui la foi assoupie de son enfance. Nous avons aussi replacé l'auteur dans le climat spirituel et philosophique des années 1900-1913, à l'heure où l'élite de la jeunesse française, hantée par les interrogations les plus angoissées, se tourne vers les "aînés" ; "les grands convertis" : Claudel, Péguy, Léon Bloy. Nous suivons les étapes de son itinéraires spirituel complexe ; ses aspirations mystiques sont incompatibles avec les doctrines et l'orthodoxieThe thesis "alain fournier his writing and culture" brings into question the legends generated by many critics and hagiographers concerning the author of le grand meaulnes. The correspondance between. A. Fournier and j. Riviere is the life line without which it is impossible to recount his formative years which teemed with bookish encounters. The authors most frequently and enthusiastically read were claudel and gide in whom it is easy to recognize the intellectual mentors who played such a vital part in unfolding fournier's creative processes and his aesthetics regarding the novel. Alain fournier's artistic education owes a lot to the preraphaelites (which he discovered during his time in the united kingdom) and to symbolist painters such as carriere and redon : the dreamlike quality which enshrouds the grand meaulnes might well have been instigated by them. M. Denis a pre-war painter belonging to a christian revival group, no doubt rekindled in fournier the faith of his childhood. In this way alain-fournier is placed in relation to the philosophical and spiritual trends of the years 1900 to 1913. This was a time when the intellectual elite of french youth, haunted by tormented self-questioning, tended to look to well-known converts such as claudel, peguy, leon bloy. In this work are laid out he complex stages of his spiritual evolution. His mystical yearnings are not compatible with doctrines and orthodoxy
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Lombes, Marc. "Recepteurs mineralocorticoides : caracterisation dans differents modeles experimentaux et purification par chromatographie d'affinite." Paris 6, 1987. http://www.theses.fr/1987PA066495.
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Purification des recepteurs mineralocortiroide (ou recepteur de l'aldosterone de type i) par chromatographie d'affinite. Ces recepteurs ont ete caracterises dans le rein du rat surrenalectomise, le colon humain normal et cancereux et le rein de lapin surrenalectomise. Parmi les agonistes et antagonistes de l'aldosterone etudies, seul un divise en 3 de la dioxycorticosteione presentait les qualites d'affinite et de specificite pour en faire un bon liganol d'affinite. Le gel synthetise avec ce derive s'est avere hautement specifique et selectif et a permis de purifier les recepteurs mineralocorticoides d'unifacteur 1000
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Uzcategui, Moncada Maria. "L'influence et représentations de la France au Venezuela : les enjeux autour des relations politiques et culturelles de 1870 à nos jours." Thesis, Lyon 2, 2015. http://www.theses.fr/2015LYO20071.
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À la différence d’autres régions du tiers monde où l’influence de la France a été déterminante et ininterrompue (Moyen-Orient, Maghreb, les anciennes colonies françaises en Afrique centrale ou encore d'autres pays de l’Amérique latine), au Venezuela, il n’y a eu de véritable politique étrangère de coopération culturelle qu’à la fin des années 1950. L'avènement du boom pétrolier pendant l'entre deux guerres a fait décliner l'influence française. À la fin de la Seconde guerre, les diplomates français prennent conscience du potentiel énergétique du Venezuela, observant qu’il est possible de remédier la perte de l’influence économique en encourageant une véritable coopération culturelle. Cette coopération culturelle va utiliser les réseaux politiques intégrés par des élites francophiles, lesquelles revendiquent une histoire commune à la France et au Venezuela. L'étude sur la longue durée permet d'observer les permanences de l'histoire politique vénézuélienne, dans lesquelles s'insèrent les éléments qui ont servi à la construction du sentiment national vénézuélien et les influences des idées françaises sur le projet de modernisation de l’État. Notre étude soulève des questions telles que l'émigration, les échanges et les relations commerciales. Nous nous sommes particulièrement intéressés à l’étude des correspondances diplomatiques car la lecture minutieuse de celles-ci permet de soulever, en filigrane, bon nombre d’éléments liés aux représentations et à l’histoire politique des deux pays. De même, elle permet d’approcher ce que les élites considèrent comme identité et affinité culturelle. Nos sources se rapportent donc, pour la plupart aux correspondances diplomatiques conservées aux archives de La Courneuve et au Ministère des Relations Extérieures à Caracas. Nous les avons croisées avec d’autres sources documentaires, visuelles et littéraires ainsi qu’avec un certain nombre d’entretiens réalisés entre la fin de l’année 2010 et le début de 2011. Notre étude s’inspire des travaux de Pierre Renouvin et Jean Baptiste Duroselle en histoire des relations internationales, revisités par Robert Frank en 2011. Nous prenons en considération le poids des images, de la culture officielle et du rayonnement des modèles culturels, dans le but de tenter de mesurer « la puissance » des États (soft power). En ce sens, la valeur que les élites vénézuéliennes donnent au rayonnement culturel et scientifique français, au XIXe et au début du XXe siècle (1870-1935), permet d’expliquer en partie pourquoi la France a réussi à renforcer sa présence au Venezuela. En effet, le déclin de l'influence culturelle française, à une époque où les relations entre les deux pays s’étaient considérablement distendues (1936-1960), est concomitant à la perte de son influence économique et commerciale. Cette évolution a donné lieu à l'institutionnalisation progressive de la stratégie de la diplomatie culturelle (1961 à nos jours). Grâce à cette stratégie la France a récupérée le terrain qu'elle avait perdu devant la concurrence commerciale anglo-saxonne. Nous avons souhaité répondre à deux questions fondamentales : dans quelle mesure les élites vénézuéliennes, « influencées » par le modèle culturel français, ont réussi à opérer des appropriations/assimilations/adaptations de ce modèle au Venezuela ? Et, en contrepartie, dans quelle mesure la construction d’un « outil culturel » franco-vénézuélien a servi les intérêts de la politique étrangère française au Venezuela ?Unlike other regions of the Third World, where the influence of France was decisive and uninterrupted (Middle East, Maghreb, former French colonies in Central Africa and other countries of Latin America), in Venezuela, there was only a real foreign cultural cooperation policy by the late 1950s. The advent of the oil boom during the interwar period caused the declining of French influence. By the end of the Second World War, French diplomats were aware of Venezuela’s energetic potential, remarking that it was possible to overcome the loss of economic influence by encouraging a genuine cultural cooperation.This cultural cooperation would use political networks joined by Francophile elites, who claimed a common history between France and Venezuela. The study on the long run allows observing the permanence of the Venezuelan political history, in which fits the elements used in the construction of the Venezuelan National sentiment and the influence of French ideas on the project of State modernization.Our study raises issues such as migration, trades and commercial relations. We are particularly interested in the study of diplomatic relationships, since a close up over these can highlight, watermark, many points related to their representations and the political history of both countries. Similarly, it allows approaching what the elite considered identity and cultural affinity. Our sources therefore relate mostly to diplomatic correspondence preserved in the files of La Courneuve and the Ministry of Foreign Affairs in Caracas. We cross checked with other documentaries, visual and literary sources as well as a certain number of interviews conducted by the end of 2010 and the beginning of 2011. Our study is based on the papers related to the history of international relations by Pierre Renouvin and Jean Baptiste Duroselle, revisited by Robert Frank in 2011. We take into consideration the contribution of images, official culture and the influence of cultural patterns, as an attempt to measure the "power" of States (soft power). On this matter, the value that the Venezuelan elites gave to the French cultural and scientific influence, in the XIX century and the beginning of XX (1870-1935), helps to explain to an extend the reason why France has managed to strengthen its presence in Venezuela. Indeed, the decline of the French cultural influence, at a time when relations between the two countries were considerably distended (1936-1960), is concomitant with the loss of its economic and commercial influence. This has resulted in the progressive institutionalization of the culturaldiplomacy strategy (from 1961 up to the present). Thanks to this strategy France has recovered part of the market share lost to the Anglo-Saxon competition.We wanted to answer two fundamental questions: To what extent the Venezuelan elites, "influenced" by the French cultural model, managed to operate appropriations / assimilation / adaptation of this model in Venezuela? And, in return, to what extent the construction of a "cultural tool" Franco-Venezuelan has served the interests of the French foreign policy in Venezuela?
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Alves, Eduardo Leandro. "Brasil, um país de fé: por que o maior país católico do mundo, também é o maior país pentecostal do mundo?" Faculdades EST, 2012. http://tede.est.edu.br/tede/tde_busca/arquivo.php?codArquivo=386.
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Esta pesquisa busca mostrar que no Brasil houve várias influências religiosas que geraram, a partir da mistura entre os povos e suas religiões, um povo místico bastante voltado para o sobrenatural. Assim, com a chegada dos Missionários Pentecostais em 1910, a Teologia Pentecostal encontrou um terreno fértil, pois a mensagem anunciada apresentava Deus intervindo de forma visível na vida do povo, curando as suas doenças, salvando a sua alma e gerando a esperança de salvação eterna. Sendo assim, investiga-se em que medida a cultura religiosa brasileira, fruto das várias etnias que formaram o que hoje chamamos de povo brasileiro, favorece a Teologia Pentecostal Clássica e o seu desenvolvimento nas várias camadas da sociedade brasileira. Para isso o texto apresenta de forma introdutória a história religiosa dos colonizadores; mostra as expressões religiosas que havia no Brasil antes da chegada dos colonizadores, assim como a religiosidade que os negros africanos trouxeram ao Brasil; relata a chegada dos primeiros missionários protestantes; analisa a chegada dos missionários pentecostais no Brasil com a Teologia Pentecostal Clássica, assim como as possíveis causas do seu crescimento no Brasil. Passando, então, a questões contemporâneas relativas ao centenário da maior denominação Pentecostal do Brasil, a Assembleia de Deus. Nesta análise supõe-se que a teoria de Max Weber da afinidade eletiva, pode ser somada com a proposta de Clifford Geertz, onde supõe-se que o desenvolvimento do Pentecostalismo Clássico no Brasil foi favorecido por questões sociais bem particulares da formação do povo brasileiro, que por afinidade, entre todas as possibilidades da teologia evangélica já presente, optou pelo Pentecostalismo, por afinidade cultural.This research aims to show that in Brazil, there were several religious influences that generated from the mixture of peoples and their religions, a very people-oriented mystical supernatural. Thus, with the arrival of Pentecostal missionaries in 1910, Pentecostal theology found fertile ground, because its message had God intervening in a visible way in the life of the people, healing their diseases, saving their soul and generating hope of eternal salvation. Therefore, we investigate to what extent the religious culture of Brazil, the fruit of the various ethnicities that formed what we now call the Brazilian people, favors the Classical Pentecostal Theology and its development in the various strata of its society. For this, the text presents in an introductory way the religious history of the settlers, shows the religious expressions practiced in Brazil before the arrival of the settlers, as well as the religiosity that black Africans brought to Brazil, reports the arrival of the first Protestant missionaries, analyzes the Pentecostal missionaries arrived in Brazil with a Classical Pentecostal Theology, as well as the possible causes of its growth in Brazil. Turning, then, to contemporary issues related to the centennial of Brazil's largest Pentecostal denomination, the Assemblies of God. In this analysis it is assumed that the theory of Max Weber's "elective affinity" can be added to the proposal of Clifford Geertz, where it is assumed that the development of Classical Pentecostalism in Brazil was favored in the forming of its people by very particular social issues that, because of affinity, among all the possibilities of the already present evangelical theology, opted by cultural affinity for Pentecostalism.
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Gavériaux, Claire. "Etude de l'interaction entre l'immunoglobuline e et son recepteur de forte affinite : mise au point d'un nouvel essai immunoenzymatique sur cellules, le celisa, importance de la n-glycosylation et de l'activation de la proteine kinase c dans l'expresion fonctionnelle de ce recepteur." Université Louis Pasteur (Strasbourg) (1971-2008), 1988. http://www.theses.fr/1988STR13014.
Full textBooks on the topic "Culture affinity"
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1909-, Bacon Francis, and Sutherland Graham Vivian 1903-, eds. Bacon and Sutherland: Patterns of affinity in British culture of the 1940s. New Haven and London: Yale University Press, 2005.
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Petrantoni, Giuseppe. Corpus of Nabataean Aramaic-Greek Inscriptions. Venice: Fondazione Università Ca’ Foscari, 2021. http://dx.doi.org/10.30687/978-88-6969-507-0.
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The impact of the Hellenization in the Ancient Near East resulted in a notable presence of Greek koiné language and culture and in the interaction between Greek and Nabataean that conducted inhabitants to engrave inscriptions in public spaces using one of the two languages or both. In this questionably ‘diglossic’ situation, a significant number of Nabataean-Greek inscriptions emerged, showing that the koin was employed by the Nabataeans as a sign of Hellenistic cultural affinity. This book offers a linguistic and philological analysis of fifty-one Nabataean-Greek epigraphic evidences existing in northern Arabia, Near East and Aegean Sea, dating from the first century BCE to the third-fourth century CE. This collection is an analysis of the linguistic contact between Nabataean and Greek in the light of the modalities of social, religious and linguistic exchanges. In addition, the investigation of onomastics (mainly the Nabataean names transcribed in Greek script) might allow us to know more about the Nabataean phonological system.
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Löwy, Michael. Redemption and utopia: Jewish libertarian thought in Central Europe : a study in effective affinity. London: Athlone Press, 1992.
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Löwy, Michael. Redemption and Utopia: Jewish libertarian thought in Central Europe : a study in elective affinity. London: Athlone Press, 1992.
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Löwy, Michael. Redemption and utopia: Jewish libertarian thought in Central Europe : a study in elective affinity. Stanford, Calif: Stanford University Press, 1992.
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Meli, Marco, ed. Le norme stabilite e infrante. Florence: Firenze University Press, 2018. http://dx.doi.org/10.36253/978-88-6453-777-1.
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Le norme stabilite e infrante. Saggi italo-tedeschi in prospettiva linguistica, letteraria e interculturale raccoglie saggi di carattere linguistico-letterario che analizzano aspetti della scrittura di autori quali Vasari e Goethe, forme di comunicazione moderna, e aspetti teorici e normativi del linguaggio. Altri saggi si concentrano sull’opera di autori italiani del Novecento (Pirandello e Atzeni), indagandone la dimensione dell’intertestualità e polifonia dei linguaggi. Vengono inoltre studiati il rapporto tra poesia concreta e poesia visiva, nonché la tecnica espressiva del linguaggio in ambito cinematografico. Il volume si chiude con un’analisi delle caratteristiche della politica culturale di entrambi i paesi, illuminandone affinità e divergenze.
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Lombardi, Sara, ed. Lettere di Margherita Guidacci a Mladen Machiedo. Florence: Firenze University Press, 2015. http://dx.doi.org/10.36253/978-88-6655-893-4.
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La raccolta delle Lettere di Margherita Guidacci a Mladen Machiedo rilegge il percorso culturale e poetico dell’autrice alla luce delle affinità con gli scrittori cattolici fiorentini, del legame con la terra dell’originario Mugello, della formazione di anglista, del lavoro critico, della costante fedeltà alla poesia (dagli esordi poetici con La sabbia e l’angelo nel 1946, fino all’ultima prova, Anelli del tempo del 1993). Il volume raccoglie le lettere che tra il 1968 e il 1989 la Guidacci scrisse a Machiedo, poeta, traduttore e insigne italianista croato, a cui la legavano comuni interessi culturali e una profonda amicizia. Il carteggio, riccamente annotato, consente di chiarire la genesi delle opere e permette il recupero di testi poetici difficilmente reperibili in Italia.
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Effective DevOps: Building a Culture of Collaboration, Affinity, and Tooling at Scale. O'Reilly Media, 2016.
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Smith, Abraham. The Bible in African American Culture. Edited by Paul C. Gutjahr. Oxford University Press, 2017. http://dx.doi.org/10.1093/oxfordhb/9780190258849.013.12.
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The chapter explores the motivations for the use of the Christian Bible in distinctive temporal arcs within African American culture. Initially, the chapter acknowledges the oddity of an African American affinity with the Bible because that Bible was deployed to support the enslavement and perpetual exploitation of African descendants in the British colonies that later became the United States. Then, it articulates three reasons for the aforementioned affinity: the availability of the Bible (especially the King James Bible) to provide a language world for personal and collective expression; the versatility or pliability of the Bible in the imagination of African Americans as they repeatedly and creatively read their own identities through the struggles of the characters of the Bible; and the perceived persuasiveness of the Bible in some of the heated debates with which the larger US public has been engaged since its inception.
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Roberts, Lee M., and Joanne Miyang Cho. Transnational Encounters between Germany and Korea: Affinity in Culture and Politics Since the 1880s. Palgrave Macmillan, 2018.
Find full textBook chapters on the topic "Culture affinity"
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Behr, Larissa, Pierre Moretti, Fabienne Anton, Cornelia Kasper, and Thomas Scheper. "Selection of High-Producing Cells Via Cell Sorting Using an Affinity Matrix." In Cells and Culture, 607–11. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_103.
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Yamada, Saichi, Yoshihiro Kamiya, Nozomu Eto, Koji Yamada, Hiroki Murakami, and Tsuyoshi Majima. "Effective Purification of Monoclonal Antibodies by Fast Flow Affinity Chromatography." In Animal Cell Culture and Production of Biologicals, 353–57. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3550-4_41.
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Schmidt, Thomas, and Arne Skerra. "The Strep-tag System for One-Step Affinity Purification of Proteins from Mammalian Cell Culture." In Methods in Molecular Biology, 83–95. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2447-9_8.
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Gressent, F., J. J. Bono, A. Niebel, H. Canut, J. V. Cullimore, and R. Ranjeva. "Characterization of a High Affinity Binding Site for NodRm Factors in Medicago varia Cell Culture Extracts." In Biological Nitrogen Fixation for the 21st Century, 213–14. Dordrecht: Springer Netherlands, 1998. http://dx.doi.org/10.1007/978-94-011-5159-7_94.
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Landgrebe, Daniel, Larissa Behr, Pierre Moretti, Johanna Walter, Frank Stahl, Cornelia Kasper, and Thomas Scheper. "A New Variant of the Affinity Matrix Method for Identification of High Producing Cells in Mammalian Cell Culture." In Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009, 139–42. Dordrecht: Springer Netherlands, 2011. http://dx.doi.org/10.1007/978-94-007-0884-6_24.
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Tachibana, Hirofumi, Kim Ji-Youn, and Hiroki Murakami. "Generation of Affinity-Variant Antibodies via the Alteration of Glycosylation in Light Chain Effected by Defined Culture Conditions of Human Hybridomas." In Animal Cell Technology: Developments Towards the 21st Century, 443–47. Dordrecht: Springer Netherlands, 1995. http://dx.doi.org/10.1007/978-94-011-0437-1_70.
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García-León, Marta, Elisa Iniesto, and Vicente Rubio. "Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures." In Methods in Molecular Biology, 297–309. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7871-7_21.
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Lübke, Christiane, Jean P. Décieux, Marcel Erlinghagen, and Gert G. Wagner. "Comparing the Risk Attitudes of Internationally Mobile and Non-Mobile Germans." In IMISCOE Research Series, 85–100. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-67498-4_5.
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AbstractMoving–particularly to a new country–is fraught with risks as migrants leave familiar legal frameworks and cultural institutions behind them. To date, little is known about the psychological determinants of international migration. This chapter helps to fill this gap by analysing data from the first wave of the German Emigration and Remigration Panel Study (GERPS) in combination with data on non-mobile individuals from the German Socio-Economic Panel Study (SOEP). The analyses presented examine whether the risk attitudes of internationally mobile Germans (‘movers’) differ from those of their non-mobile counterparts (‘stayers’). The results show that–with control for key socio-demographic and socio-economic determinants of risk affinity–both emigrants and remigrants report a significantly higher willingness to take risks than stayers. Risk affinity differs within the group of internationally mobile individuals: Emigrants moving to geographically and culturally distant non-European countries report higher risk affinity than those moving to Germany’s neighbouring countries. Emigrants with multiple previous emigration periods are also more willing to take risks. These findings suggest that voluntary emigration from wealthy countries like Germany is only partly a matter of living conditions. Rather, (repeated) emigration seems to be a matter of personality and an expression of a more adventurous lifestyle.
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Wu, Fan. "Cultural affinity of managers of international joint ventures: an experimental study." In Implicit Incentives in International Joint Ventures, 59–86. Wiesbaden: Gabler Verlag, 2012. http://dx.doi.org/10.1007/978-3-8349-7076-3_4.
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Monteiro, Juliana, Carla Morais, and Miguel Carvalhais. "NOOA: Maintaining Cultural Identity Through Intergenerational Storytelling and Digital Affinity Spaces." In Interactive Storytelling, 376–79. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-71027-3_47.
Full textConference papers on the topic "Culture affinity"
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Nishi, Masanori, Jun Kobayashi, Masayuki Yamato, Akihiko Kikuchi, Katsumi Uchida, Hirofumi Yajima, and Teruo Okano. "Immobilization of biomolecules onto thermoresponsive culture dishes by affinity binding." In 2006 IEEE International Symposium on MicroNanoMechanical and Human Science. IEEE, 2006. http://dx.doi.org/10.1109/mhs.2006.320283.
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Skorniakova, S. S. "Affinity Of Gender Stereotypes And Political Culture: From Discrimination To Equal Rights." In RPTSS 2017 International Conference on Research Paradigms Transformation in Social Sciences. Cognitive-Crcs, 2018. http://dx.doi.org/10.15405/epsbs.2018.02.133.
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Piovella, F., R. Lombardi, M. Vigotti, A. B. Federici, P. M. Mannucci, and E. Ascari. "VON WILLEBRAND FACTOR MULTIMERS IN CULTURED HUMAN ENDOTHELIAL CELLS: COMPARISON BETWEEN CELLULAR STORAGE POOL AND SUPERNATANT." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644100.
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The multimeric composition of von Willebrand factor (vWf) from cultured human endothelial cells (h.e.c.) has been compared with the multimeric composition of vWf from h.e.c. culture supernatant, human normal platelets and plasma. H.e.c. were derived from umbilical cord veins by collagenase digestion, seeded in culture flasks and grown to confluence in TC 199 culture medium, supplemented with 20% foetal calf serum (FCS). At confluency, cells were harvested by rubber policeman, resuspended in 500 |o.l HBSS with protease inhibitors and stored with culture media until assay. H.e.c. and platelet lysates were obtained by freezing-and-thawing (5 times) and by the addition of 1% Sodium Dodecyl Sulphate (SDS) before centrifugation at 10,000 x g for 20 min at 20°C. Plasma, culture supernatants and cell lysates supernatants were run under the same conditions in an SDS 1.4%, LGT-agarose electrophoresis system using a discontinous buffer. Multimeric patterns were shown by radiolabelled affinity purified rabbit anti-human polyclonal antibody. In all the experiments cultured h.e.c. supernatants exhibited all the set of multimers of vWf usually observed in normal plasma. When vWf from cultured h.e.c. extracts was analyzed, a set of multimers with higher molecular weight was shown, with a pattern very similar to platelet's. We conclude that vWf stored in h.e.c. compartments is characterized by higher molecular weight multimers than culture supernatants. This behaviour recalls the differences recorded between plasma and platelet vWf.
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Zaytsev, Pavel. "Modernism, Publicness, Zombification: Gestalt of "Worker" by E. Junger, And Phenomena of Contemporary Exploitative Culture." In The Public/Private in Modern Civilization, the 22nd Russian Scientific-Practical Conference (with international participation) (Yekaterinburg, April 16-17, 2020). Liberal Arts University – University for Humanities, Yekaterinburg, 2020. http://dx.doi.org/10.35853/ufh-public/private-2020-17.
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The need for researching into ideological sources of contemporary exploitative culture is necessitated by both the outer edge of its interaction with other triggers of modernity, and the inner edge consisting in the answer to the following question: ‘what is an exploitative culture?’. The modernism era gave rise not only to global mass culture, but diverse oppositions of ‘privacy’ and ‘publicity’ categories in their key anthropological images. It seems to us to be no coincidence that exploitative culture is presented by researchers primarily in the anthropological dimension of race and sex. While considering the heroic characters proposed to be scaled for the era of modernism, it is necessary to account for the invariative content, which was reflected in gestalt of the ‘worker’ by E. Junger, and its particular historical variations. We pay our attention to the pedagogical system suggested by A. Makarenko, and the system of fostering actors of the future by V. Meyerhold as projects of the taylorisation of school and theater. The contemporary culture which, as a result of racial protests in the USA, has tended to be attributed with the predicate ‘exploitative’ reveals the exploitative meanings of the worker’s gestalt in the image of the zombie and the phenomenon of zombification associated with it. As a result of this study, conclusions were drawn regarding the continuity of the anonymous image of the ‘worker’ E. Junger and the film image of the zombie as one of modern culture’s most demanded anonymous generalised characters of the masses. Their affinity is as follows: the ‘worker’ of E. Junger is not a social, much less an economic category, it is the most common anthropological metaphore of ‘generic attributes’ to characterise the modernism era, like a zombie character in contemporary mass culture. However, if gestalt of ‘worker’ by E. Junger means the totality of creation of a new world, then the zombie character in contemporary mass culture is associated with the totality of devastation.
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Furihata, Kenichi, Diane J. Nugent, Amy L. Bissonette, Elizabeth Vokac, and Thomas J. Kunicki. "PRODUCTION OF HUMAN MONOCLONAL ANTIBODIESSPECIFIC FOR PLATELET MEMBRANE GLYCOPROTEIN IIIa." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643705.
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Human monoclonal antibodies specific for platelet membrane glycoproteins (GPs) arepotentially important reagentsfor studies of the immunogenicity of membrane glycoproteins. A human monoclonalautoantibody, 5E5, reactive with plateletGPIIIa has been developed (Nugent, et al.,Blood, 1987, in press). In this report, we describe the production of additional human monoclonal antibodies specific for GPIIIa. Peripheral blood lymphocytes fromone patient with post-transfusion purpur(PTP) and one woman who had delivered an infant with neonatal alloimmune thrombocytopenic purpura (NATP) were used as a source of antigen-specific lymphocytes. A B-lympho-cyte-enriched population was transformed with Epstein Barr virus, strain B95-8, and cultured in microtiter plates. After two weeks, culture supernatants were screened by an antigen-capture ELISA wherein murine monoclonal antibody specificfor the GPIIb-IIIa complex was used to holdcorresponding antigen from a lysate ofnormal platelets. B-lymphoblastoid cell lines producing IgG and/or IgM antibodies were expanded and either cloned by limiting dilution technqiue or hybridized with a HAT-sensitive, ouabain-resistant heterohybrid fusion line, F6, using polyethylene glycol. Hybridomas were selected in medium containing HAT andouabain. After twoweeks, hybridomas producing anti-GPIIb and/or anti-GPIIIa antibody were cloned by limiting dilution. Culture supernatants from cloned B-lymphoblastoid cell lines and cloned hybridomas were rescreened by ELISA wherein affinity-purified GPIIIa or other platelet GP weredirectly conjugatedto microtiter plates. One IgM antibody produced by acloned B-lymphoblastoid cellline (CH16) andtwo IgG antibodies produced bycloned hybridomas(Del5.19 and Del5.23) were shownto react with GPIIIa but not other GP. Further characterization of these human monoclonal antibodies, produced continuouslyin culture now for four months, is in progress.
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Eguchi, Y., Y. Sakata, M. Matsuda, and K. Kondo. "EFFECT OF THROMBIN-CLEAVAGE OF PRO-UROKINASE ON ITS AFFINITY TO FIBRIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644413.
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Pro-urokinase (PUK) has been reported to bind to fibrin-Sepharose specifically but not to fibrin clot. As thrombin (TH) clotted fibrinogen and cleaved the Arg 156-Phe 157 peptide bond of PUK simultaneously, we tested effect of this cleavage on the binding of PUK to fibrin by using high molecular weight urokinase (HMUK), PUK purified from culture media of human kidney cell (nPUK) and two kinds of recombinant PUK (rPUK) expressed in genetically transformed E. Coli. One rPUK designated as P0 lacks the carbohydrate side chain present in nPUK but has the same amino acids sequence as nPUK does. The other rPUK designated as P3 is a mutant whose Phe 157 was replaced by Asp so as to be resistant against the proteolytic cleavage by TH. The rate of conversion of single-chain 125I labeled PUK (125I PUK) to two-chain form by TH or plasmin (PM) was determined by both amidolytic activity toward S-2444 and autoradiography of polyacrylamide gel under reducing conditions. Although 100 U/ml TH did not cleave 125I P3 in 2h at 37°C, 10 U/ml TH completely cleaved H00 U/ml 125I nPUK and PO instantaneously. 125I PUK, which had been totally or partially cleaved by either TH or PM, was mixed with fibrinogen in the presence of aprotinin. Then the mixture was clotted with 2.5 mM Ca2+ and 0.5 U/ml TH which cleaved PUK only a little for 10 min. Clots were obtained by clot-syneresis and washed with Tris-buffer containing albumin, aprotinin and hirudin. The bound PUK was calculated by measuring the radioactivity of the clot and the supernatant. Although the binding of nPUK and PO to fibrin decreased from 12% to 1% as cleavage by TH or PM proceeded, that of P3 to fibrin decreased only when two-chain conversion was processed by PM. HMUK did not bind to fibrin. These results strongly suggest that single-chain PUK binds to fibrin and imply that P3, which is TH-resistant, may be useful as an activator in the presence of TH surrounding the clot.
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Sakuragawa, N., S. Saitoh, and K. Takahashi. "INTERACTION BETWEEN CULTURED ENDOTHELIAL CELLS AND ABNORMAL ANTITHROMBIN III "TOYAMA"." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644366.
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Purpose: Abnormal antithrombin III(AT-III)Toyama showed non-affinity to heparin and heparinoid to show loss of immediate antithrombin activity. On the endothelial cells, there are heparinoids including heparan sulfate. We investigated on the interaction between cultured endothelial cells and abnormal AT-III"Toyama" from the viewpoint of antithrombin activity.Materials and methods: (1) Endothelial cell culture:^125I-labelled normal and abnormal AT-III were placed on the washed endothelial cultured cells in 0.2 ml of RPMI-1640 medium for 15 min at 37°C. The medium was suctioned off and the cell layer was washed with Hank's balanced salt solution. The cells were incubated with 1 ml of heparin(3 ug/ml) for 15 min at 4°C. The radioactivity in the supernatant was counted, and represented AT-III which bound to the cells surface. (2) Antithrombin activity: 0.23 ml of thrombin solution^ U/ml) and 0.03 ml of normal or abnormal AT-III plasma were mixed, and incubated on the cultured cell surface for 5 min at room temperature. The residual thrombin activity was assayed by 0.3 ml of the substrate (S-2238) solution(0.8mM)for 5 min. After these procedures,2 ml of 2% citric acid solution was added to stop the reaction, and 0D(405 nm) was recorded.Results: Abnormal AT-III showed reduced binding-activity to cultured cells to one fifth compared with normal AT-III, and the residual thrombin activity in the abnormal was higher compared with that in normal plasma.Conclusion: Abnormal AT-III showed less binding activity to the cultured endothelial cells, and less thrombin neutralizing activity to show thrombogenic tendency.
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Brewer, Bryson M., Yandong Gao, Rebecca M. Sappington, and Deyu Li. "Microfluidic Molecular Trap: Probing Extracellular Signaling by Selectively Blocking Exchange of Specific Molecules in Cell-Cell Interactions." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-64489.
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Communication among cell populations is achieved via a wide variety of soluble, extracellular signaling molecules [1]. In order to investigate the role of specific molecules in a cellular process, researchers often utilize in vitro cell culture techniques in which the molecule under question has been removed from the signaling pathway. Traditionally, this has been accomplished by eliminating the gene in the cell that is responsible for coding the targeted ligand/receptor by using modern DNA technology such as gene knockout; however, this process is expensive, time-consuming, and labor intensive. Previously, we have demonstrated a microfluidic platform that uses a semi-permeable barrier with embedded receptor-coated nanoparticles to selectively remove a specific molecule or ligand from the extracellular signaling pathway in a cell co-culture environment [2]. This initial proof-of-principle was conducted using biotinylated nanoparticles and fluorescently tagged avidin molecules, as the avidin/biotin complex is the strongest known non-covalent interaction between a protein and a ligand (Dissociation constant kd = 10−15 M). Also, the trap was only effective for short time periods (<15 min) because the high concentration of fluorescently tagged avidin molecules required for visualization quickly saturated the barrier. However, nearly all biologically relevant ligand-receptor interactions have lower binding affinities than the avidin-biotin complex, with dissociation constants that are larger by several orders of magnitude. In addition, many in vitro cell culture experiments are conducted over multiple hours or days. Thus, a practically useful molecular trap device must be able to operate in a lower binding affinity regime while also lasting for extended time periods. Here we present results in which a biotinylated-particle barrier was used to successfully block lower concentrations of fluorescently tagged avidin for multiple days, showcasing the applicability of the device for long term experiments. In addition, we introduce a modified molecular trap in which the protein A/goat IgG complex was used to demonstrate the effectiveness of the platform for lower binding affinity protein-ligand interactions. These results indicate the potential usefulness of the microfluidic molecular trap platform for probing extracellular signaling pathways.
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Kim, Jong-Hoon, Woon-Hong Yeo, Zhiquan Shu, Shinnosuke Inoue, Kieseok Oh, Dayong Gao, Jae-Hyun Chung, and Kyong-Hoon Lee. "Tip Enrichment System for Rapid Screening of Mycobacterium Tuberculosis." In ASME 2010 International Mechanical Engineering Congress and Exposition. ASMEDC, 2010. http://dx.doi.org/10.1115/imece2010-38403.
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Rapid detection of tuberculosis (TB) has been critically demanded over the last century. To detect TB, numerous methods screening Mycobacterium tuberculosis (MTB) have been developed. However, the methods still have challenges of rapid and specific enrichment of MTB. In this study, we present a novel specific enrichment method of MTB using a microfabricated tip. Through our simulation study, a wavy-shaped microtip is designed to enhance capturing efficiency of bacteria. Using an optimized tip, bacteria are attracted by dielectrophoresis and captured by affinity binding and capillary action. In experiment, a surrogate marker of MTB, Bacilli Calmette-Guerin (BCG), is enriched with the micromachined tip. When a microtip decorated with capturing antibodies is used, BCG cells spiked in saliva are detected at the concentration of 5×105 CFU/mL within 20 minutes. The tip enrichment system demonstrates the potential for rapid, culture-free detection of MTB in a raw sample.
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Giddings, J. C. "AN IMMUNORADIOMETRIC ASSAY (IRMA) FOR HUMANTHROMBOMODULIN." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643963.
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Thrombomodulin was separated from detergent-soluble fractions of human placenta using a combination of DEAE-Sepharose and thrombin-Sepharose chromatography. The final product demonstrated one major band (Mr approximately 100000) and three minor bands (Mr 40000 - 80000) on SDS-polyacrylamide gel electrophoresis. The purified protein markedly enhanced the rate of activation of human protein C by thrombin in the presence of calcium ions. Polyclonal antibodies to the isolated thrombomodulin were raised in rabbits and were shown to inhibit thrombin co-factor activity. Immunofluorescence of fibroblasts and of human umbilical vein endothelial cells in culture demonstrated that thrombomodulin antigen was specifically located in endothelial cell membranes. Affinity purified antibody was labelled with 125I and was used to establish an immunoradiometric assay (IRMA). The method was sensitive to approximately 10.0μg purified thrombomodulin per litre. The concentration of thrombomodulin in detergent-solubilised endothelial cells obtained at intervals during primary culture was proportional to the number of cells harvested for assay and appeared to reflect cell growth. Confluent cells from four experiments (approximately 2 × 106 cells per culture dish) contained on average 8.4ng thrombomodulin. Incubation of confluent endothelial cells with medium containing 1 unit per ml human α-thrombin for 10 mins at 37°C reduced the amount of cellular thrombomodulin detected in this assay by up to 30%. Assays of human plasma confirmed that low levels of thrombomodulin are present in normal circulating blood. A mean level of 160μg per litre was detected in 20 normal donors. The levels of thrombomodulin antigen in 10 normal serum were not significantly different from those in the corresponding normal plasma. Preliminary results illustrated that increased levels of thrombomodulin might be found in the plasma of some patients with a variety of clinical disorders. The data suggest that quantitative assays of thrombomodulin might provide a useful index of endothelial disturbances in vivo.