Dissertations / Theses on the topic 'Cultural differentiation'
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Carpenter, John Philip 1957. "El ombligo en la labor: Differentiation, interaction and integration in prehispanic Sinaloa, Mexico." Diss., The University of Arizona, 1996. http://hdl.handle.net/10150/282237.
Full textBruwer, Helena Dorothea. "The Canadian education system with special reference to cultural differentiation / Helena Dorothea Bruwer." Thesis, Potchefstroom University for Christian Higher Education, 1986. http://hdl.handle.net/10394/8754.
Full textThesis (MEd)--PU vir CHO, 1987
Diederich, Marcia C. "Cultural determinants in Chinese and American preschool children's understanding of physical laws and social rules." Menomonie, WI : University of Wisconsin--Stout, 2008. http://www.uwstout.edu/lib/thesis/2008/2008diederichm.pdf.
Full textEriksson, Linn, and Hanna Santesson. "Organizational Culture in a Remote Setting : A Qualitative Study on Organizational Culture and the Effects of Remote Work." Thesis, Uppsala universitet, Företagsekonomiska institutionen, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-448645.
Full textWang, Chongying. "A cross-cultural study of metaphoric understanding in English and Chinese children and adults from a developmental and cognitive perspective." Thesis, University of Oxford, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670038.
Full textHills, Mils. "Modes of association and differentiation in Mauritius : an account of identity in a situation of socio-cultural heterogeneity." Thesis, University of St Andrews, 1999. http://hdl.handle.net/10023/6455.
Full textOuthwaite, Deborah Emily. "Educational leadership in the International Baccalaureate : critical reflections on modern elite formation and social differentiation." Thesis, University of Derby, 2017. http://hdl.handle.net/10545/621613.
Full textPhillips, Marianne, and edu au jillj@deakin edu au mikewood@deakin edu au wildol@deakin edu au kimg@deakin. "The Internationalisation of Singapore Television: Singaporean Regional and Global Perspectives and Contexts." Deakin University. School of Literary and Communication Studies, 2001. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20040818.141118.
Full textHung, Auris Huang. "The concept of differentiated oneness and implications for Asian American families." Theological Research Exchange Network (TREN), 2004. http://www.tren.com.
Full textMetni, Lena. "Nivågruppering i grundskolans tidigare år : Hur och varför används den i matematikundervisningen." Thesis, Södertörns högskola, Lärarutbildningen, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-14798.
Full textHallerström, Jacob. "Konstverkets roll i samtida reklam : Funktioner, meningsskapande och en jämförelse mellan svensk och singaporiansk reklam." Thesis, Stockholms universitet, Institutionen för mediestudier, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-165559.
Full textLemaître, Mathieu. "Ressources patrimoniales culturelles et développement touristique." Thesis, Toulouse 2, 2015. http://www.theses.fr/2015TOU20036/document.
Full textThis thesis investigates the determinants of cultural heritage tourism development. Part one is devoted to the notion of heritage, as well as economic issues related to its valorisation. Part two provides a theoretical and conceptual framework that takes into account the specific nature of heritage, and addresses heritage market mechanisms through the notions of absolute and differentiative advantage. Special attention is also being paid to heritage activation process. In part three, this research questions the relationship between cultural resources of outstanding universal value, valorisation through a proven labelling strategy (or at least portrayed as such), and major tourist attraction status, through the analytical lens of UNESCO world heritage list. Econometric modelling is then employed to study the relative contribution of keys cultural heritage features upon tourism and socio-economic performance at the Midi-Pyrénées’ cantonal scale. Our results show that heritage’s potential impact on tourism development is strongly related to its own intrinsic cultural value. However, the real impact of heritage depends more on the way resources are being used, and on the economic environment in which these resources are being brought into the market. Even though labels hold a central position in tourism development policy, the tests we conducted do not provide any conclusive evidence of a quantifiable economic impact. Labelling strategies may act as a catalyst for tourism and economic development, yet expected benefits remain highly contingent upon the sites’ pre-labelling economic profile, as well as the nature of the interventions that accompanies designation
Freyer, Nora [Verfasser]. "Optimizing culture conditions for hepatic differentiation of human induced pluripotent stem cells : from 3D culture systems to co-cultures / Nora Freyer." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2018. http://d-nb.info/1160514968/34.
Full textBai, Qiang. "Human pluripotent stem cells in In vitro conditions : differentiation and genomic instability." Thesis, Montpellier 1, 2013. http://www.theses.fr/2013MON1T007/document.
Full textHuman pluripotent stem cells (hPSC) are the stem cells capable to self-renew and also to differentiate into all the cell types. These cells can be derived from embryos (for human embryonic stem cells, hESC) but also be obtained by reprogramming the differentiated somatic cells (for human induced pluripotent cells, hiPSC). The hPSC become central stakes of science, medicine and economy, particularly for genetic and rare diseases. In fact, they open up the new perspectives to the novel treatment strategies by remodeling human genetic diseases in vitro and at the same time they are a potentially unlimited cell source for cell therapy for especially degenerative diseases. Meanwhile, the hPSC in vitro culture is one of the most important steps before passing to the clinic applications and in fundamental research, as the proliferation and pluripotency can only be maintained in culture condition as well as many differentiation methods. My PhD work was concentrated on the hPSC in vitro culture. At first, I modeled human trophoblastic development and its differentiation pathway in vitro by modulating the parameters of culture, especially the concentration of BMP4. This work permitted clarifying the first cell lineage bifurcation in early human embryonic development. Secondly, my word was focalized on the phenotypic and genomic changes of hPSC during the in vitro culture. I demonstrated that the use of some passaging protocols in culture, particularly complete cell dissociation by trypsin, was translated by very early acquisitions of chromosomal and sub-chromosomal abnormalities, and that the appearance of sub-chromosomal abnormalities could precede chromosomal abnormalities. The consequences of these observations are important for the hPSC culture research: (1) the use of complete cell-dissociation passaging should be definitively abandoned, including the suspension culture, and (2) the genetic analyses with higher resolution should be added to validate a culture technic
Mayaud, Isabelle. "Sciences de la musique sans frontières ? : Contribution à une sociologie du processus de primitivisation." Thesis, Paris 8, 2018. http://www.theses.fr/2018PA080007.
Full textIn this dissertation, I analyse how, in the modern period, the different scientific domains dealing with music were divided, and how, at the same time, musical repertories were organised into a hierarchy. This research, focused on the French case, is based on a socio-historical enquiry and on several sources dating from the beginning of the seventeenth to the mid-twentieth century. Those sources are both manuscript and printed, and range from administrative documents, scientific and museum archives, conference proceedings and other printed sources related to the Universal Exhibitions, to archives from the publishing sector and other pieces related to the collection and curating of musical instruments, songs and audio recordings. The following methods were mobilised : lexical analysis, textual sociology, databases and historical ethnography. The enquiry emphasizes a configuration of the process of making music a part of national heritage by the French State, which is also a long-term process of social differentiation through the music. Collecting and curating operations of musical objects were initiated by the Second Empire and consolidated by the Third Republic. These operations have contributed to make certain repertories anhistorical, kept in a zone below history. This separation is analysed as a symbolic domination system, which was enacted by several administrations (Public Instruction, Trading and Industry, Fine Arts, Colonies), produced and reproduced by different agents commissioned by the State (teachers and professors, academicians, curators, territorial leaders)
Seeley, Marguerite R. "Developmental toxicity of alkylating agents in differentiating cell cultures /." Thesis, Connect to this title online; UW restricted, 1996. http://hdl.handle.net/1773/8464.
Full textField, R. P. "Primary culture of uterine cells : Markers of growth and differentiation." Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.372403.
Full textThomas, Leigh Sara. "Development of a culture system to study human trophoblast differentiation." Thesis, University of Cambridge, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621203.
Full textColetta, Riccardo. "Manipulating growth and differentiation of embryonic intestine in organ culture." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/manipulating-growth-and-differentiation-of-embryonic-intestine-in-organ-culture(ad43b8a7-2499-4813-a1de-c46a24a079aa).html.
Full textKarandikar, Atul. "Streptomyces coelicolor A3(2) : growth and differentiation of surface grown cultures." Thesis, Liverpool John Moores University, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242153.
Full textGorodeski, George Israel. "Retinoids and steroid hormones regulate differentiation of cultured human ectocervical cells." Case Western Reserve University School of Graduate Studies / OhioLINK, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=case1054845951.
Full textHopley, J. P. "Toxicity studies in a differentiating epidermal keratinocyte culture." Thesis, University of Surrey, 1986. http://epubs.surrey.ac.uk/847531/.
Full textSargent, Carolyn Yeago. "Effects of hydrodynamic culture on embryonic stem cell differentiation: cardiogenic modulation." Diss., Georgia Institute of Technology, 2010. http://hdl.handle.net/1853/34710.
Full textJones, Erin Boote. "Effects of substrate and co-culture on neural progenitor cell differentiation." [Ames, Iowa : Iowa State University], 2008.
Find full textCapra, J. (Janne). "Differentiation and malignant transformation of epithelial cells:3D cell culture models." Doctoral thesis, Oulun yliopisto, 2018. http://urn.fi/urn:isbn:9789526218236.
Full textTiivistelmä Epiteelisolut ovat erikoistuneet toimimaan rajapintana elimen ja ympäristön välillä. Ihmisten yleisin syöpä on epiteelisoluista alkunsa saanut karsinooma. Tämän tutkimuksen tarkoituksena oli ymmärtää Madin-Darby-koiran munuaisen solujen (MDCK) erilaistumista ja pahanlaatuistumista sekä analysoida sähköfysiologisia tekijöitä, jotka säätelevät näiden solujen kuljetustoimintaa. Erityisenä kiinnostuksen kohteena oli erilaisten kasvuympäristöjen vertailu. Farmakologisten aineiden tai basaalisen, solunulkopuolisen nesteen koostumuksen vaikutusta MDCK-solujen, -kystan sekä luumenin kokoon tutkittiin valomikroskooppisten aikasarjojen avulla. Tulokset osoittivat MDCK-solujen olevan kykeneviä sekä veden eritykseen että absorptioon, niin hyperpolarisoivassa kuin depolarisoivassakin ympäristössä. Basaalisen nesteen osmolaliteetin muutosta ei tarvittu. Nämä tulokset osoittavat MDCK-solujen olevan hyvä munuaisen tutkimuksen perusmalli. Seuraavaksi analysoitiin kaksi- ja kolmiulotteisten (2D ja 3D) viljely-ympäristöjen vaikutusta ei-transformoitujen MDCK-solujen ja lämpötilaherkkien ts-Src-transformoitujen MDCK-solujen geenien ilmentymiseen sekä yhden onkogeenin aktivoimisen aikaansaamia muutoksia. Microarray-analyysi osoitti apoptoosin estäjän, surviviinin, ilmentymisen vähenemisen, kun kasvuympäristö vaihdettiin 2D-ympäristöstä 3D-ympäristöön. Koska surviviinin väheneminen on normaali tapahtuma aikuisissa kudoksissa, voitiin todeta, että 3D-ympäristössä kasvatetut solut ovat lähempänä luonnonmukaista olotilaa kuin 2D-ympäristössä kasvaneet. Src-onkogeeni sai aikaan soluliitosten hajoamisen, mutta ei vähentänyt E-kadheriinin ilmentymistä. Tutkimuksen viimeinen osa keskittyi surviviinin ilmentymistä säätelevien tekijöiden analysoimiseen ja surviviinin merkitykseen solujen eloonjäämiselle. 3D-ympäristössä kasvaneet MDCK-solut eivät kärsineet apoptoosista edellyttäen, että solut pysyivät kosketuksissa soluväliaineeseen. Jos solut irtautuivat soluväliaineesta, ne päätyivät herkemmin apoptoosiin kuin surviviinia ilmentävät ts-Src MDCK-solut. Mikäli solujen väliset liitokset pakotettiin avautumaan, solut joutuivat apoptoosiin, vaikka ne olivat kosketuksissa soluväliaineeseen. Yhteenvetona nämä tulokset korostavat solujen kontaktien merkitystä: MDCK-solut tarvitsevat soluväliainekontakteja erilaistumiseen ja solujen välisiä kontakteja välttyäkseen apoptoosilta
Chen, Jim-Ray. "Hepatocytic differentiation of normal but not neoplastic cultured rat pancreatic duct cells." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=28996.
Full textThe technique of in vivo implantation of cells to subcutaneous and intraperitoneal sites is used as it not only reveals the intrinsic potential of the implanted cells but also reflects the effects of the microenvironment on phenotypic expression.
The results of these studies indicate that when implanted in vivo normal propagable cultured cells derived from the duct epithelium of adult rat pancreas develop phenotypic features of a hepatocyte, and that the extent of this phenotypic expression is influenced by the microenvironment in which these cells are implanted. When localized subcutaneously, the cells displayed partial differentiation toward hepatocytes but retained some of their ductal phenotype. In contrast, when the same cells were implanted intraperitoneally, they expressed the full phenotypic properties of mature hepatocytes. Both spontaneously- and chemically-transformed pancreatic ductal cell lines did not display phenotypic differentiation along the hepatocytic lineage after in vivo implantation. It is concluded that (1) pancreatic ductal cells can be the progenitor cell for pancreatic hepatocytes; (2) Neoplastic transformation of these cell lines results in partial or total loss of hepatoctyic differentiation.
Eccles, Bree A. "Effect of Cannabinoids on Osteogenic Differentiation of Cultured Vascular Smooth Muscle Cells." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/honors/392.
Full textChesley, Colin G. "Merging Cultures: Organizational Behavior, Leadership, and Differentiation in a Health System Merger." Digital Commons @ East Tennessee State University, 2017. https://dc.etsu.edu/etd/3271.
Full textLiu, Ning. "Expansion and Neural Differentiation of Embryonic Stem Cells in Three-Dimensional Cultures." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1262281522.
Full textRotti, Pavana Gururaj. "3D differentiation enhances the efficiency of differentiation of human induced pluripotent stem cells to insulin producing cells." Thesis, University of Iowa, 2014. https://ir.uiowa.edu/etd/2266.
Full textBarber, M. C. "Studies on the growth and differentiation of the mammary gland in culture." Thesis, University of Reading, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380923.
Full textKhayat, Ghazaleh. "Low frequency stimulation of stem cells in dynamic culture modulates differentiation pathways." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=119594.
Full textToutes les cellules vivantes, selon leur fonctions physiologiques, sont soumises à différentes stimulations mécaniques. L'ampleur et la fréquence de ces stimulations mécaniques varies considérablement d'un organe à un autre. Les stimulations oscillantes dues notamment à la marche, la respiration et la circulation sanguine sont largement étudiées. Par contre, les travaux concernant les stimulations a très faibles fréquences sont rare. Cette recherche examine les effets sur différents types de molécules, des stimulations mécaniques à relativement basse fréquence, à l'échelle moléculaire. Tout au long du travail présenté ici, l'accent a été mis sur la différenciation des cellules souches et la dedifférenciation des cellules primaires. Les résultats suggèrent que la pratique d'activités extrêmement lentes, à savoir les mouvements à basse fréquence, affectent, de manière significative, le mécanisme de différentiation des cellules souches. En outre, il a été constaté que les mouvements lents à la surface des cultures améliorent les caractéristiques phénotypiques des cellules primaires.
Wataya, Takafumi. "Minimization of Exogenous Signals in ES Cell Culture Induces Rostral Hypothalamic Differentiation." Kyoto University, 2008. http://hdl.handle.net/2433/124243.
Full textYarwood, Stephen J. "The cyclic amp signalling system as a regulator of preadipocyte differentiation." Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.362948.
Full textWetten, Andrew C. "The influence of light regime on growth and differentiation of cultured tomato explants." Thesis, Oxford Brookes University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315398.
Full textMillman, Jeffrey Robert. "Effects of low oxygen culture on pluripotent stem cell differentiation and teratoma formation." Thesis, Massachusetts Institute of Technology, 2011. http://hdl.handle.net/1721.1/65759.
Full textCataloged from PDF version of thesis.
Includes bibliographical references (p. 163-175).
Pluripotent stem cells (PSC) hold promise for the study of embryonic development and the treatment of many diseases. Most pluripotent cell research is performed in incubators with a gas-phase oxygen partial pressure (p02) of 142 mmHg. However, embryonic cells in early development are exposed to a local P02 of 0-30 mmHg, and the effects of such conditions on differentiating PSC are poorly understood. Residual PSC within differentiated populations are problematic because of their potential to form tumors in vivo. This is a major safety issue that must be overcome before PSC-based therapies can be used in the clinic. In this study, we differentiated mouse and human embryonic stem cells and mouse induced pluripotent stem cells at different defined P02 on highly oxygen-permeable silicone rubber culture dishes and assessed differentiation to the three germ layers, endoderm, ectoderm, and mesoderm and to cardiomyocytes and assessed residual PSC within differentiated populations. Low P02 drastically affects differentiation of PSC to the three germ layers and cardiomyocytes. Overall, differentiation was higher to endoderm, lower to ectoderm, and higher or the same to mesoderm. Differentiation to cardiomyocytes was greatly enhanced without the need for purification, possibly by lineage selection via increased Mesp1 and Mesp2 expression. Understanding the effects of P02 during differentiation is an important step towards the development of protocols for regenerative medicine. Control of P02 to physiological levels typical of the developing embryo reduced the fraction of PSC within, and the tumorigenic potential of, differentiated populations. Culture under differentiating conditions at low PO2 reduced measured pluripotency markers by up to four orders of magnitude. Upon implantation into immunocompromised mice, low PO2-differentiated PSC either did not form tumors or formed tumors at a slower rate than high PO2 PSC. Low PO2 differentiation could be combined with cell sorting for improved benefits. Low PO2 culture alone or in combination with other methods is a potentially straightforward method that could be applied to future cell therapy protocols to minimize the possibility of tumor formation.
by Jeffrey Robert Millman.
Ph.D.
Krassowska, Anna Katharina. "Haematopoietic differentiation of embryonic stem cells by aorta-gonad-mesonephros region co-culture." Thesis, University of Edinburgh, 2004. http://hdl.handle.net/1842/29206.
Full textKhetani, Salman R. "Micropatterned co-cultures of hepatocytes and nonparenchymal cells mechanisms of differentiation, dynamics and applications /." Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2006. http://wwwlib.umi.com/cr/ucsd/fullcit?p3204579.
Full textTitle from first page of PDF file (viewed Apr. 4, 2006). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 242-264).
Zandstra, Peter William. "Cytokine-dependent regulation of human hematopoietic cell self-renewal and differentiation in suspension cultures." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq25194.pdf.
Full textIto, Akira. "Culture temperature affects redifferentiation and cartilaginous extracellular matrix formation in dedifferentiated human chondrocytes." 京都大学 (Kyoto University), 2015. http://hdl.handle.net/2433/199220.
Full textCho, Hsin-Hua. "Differentiation of human embryonic stem cells into pancreatic progenitors using chemically defined culture systems." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608076.
Full textSoh, Boon Seng. "Optimization of Human Embryonic Stem Cells Culture and their Differentiation towards the Lung Lineage." Thesis, Imperial College London, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.516176.
Full textShann, Linzi H. "Protein kinase Cα and the regulation of ovine articular chondrocyte differentiation/dedifferentiation in culture." Thesis, University of York, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.273904.
Full textHashimura, Yasunori 1980. "Fundamental differentiation and growth characterization of murine embryonic stem cells in varied culture conditions." Thesis, Massachusetts Institute of Technology, 2004. http://hdl.handle.net/1721.1/28529.
Full textIncludes bibliographical references (leaves 81-83).
Although embryonic stem (ES) cells and their pluripotent capability have been elucidated for decades, little study has been done on obtaining the pluripotency profile of ES cells in the incipient stages of differentiation. In this research, an ES cell line with transfected green fluorescent protein (GFP) co-expressed by an Oct-4 promoter was analyzed by fluorescence-activated cell sorter (FACS) to obtain such profile. As Oct-4 is an ES cell differentiation marker whose expression varies with pluripotency, GFP expression could simply be measured in these cells to determine how pluripotent they are as a population. The differentiation characterization of ES cells was also conducted with different culture conditions of reduced serum and glucose concentrations both in the presence and absence of leukemia inhibitory factor (LIF) which prevents spontaneous differentiation, as well as at varied LIF concentrations and seeding densities. In addition, fundamental growth kinetic and metabolic profiles were obtained to get a more complete picture of how ES cells behave under these varied culturing conditions. The doubling time (t[sub]d) of R1 Oct4-GFP cell line was found to be 13 hours in LIF⁺ culture and 8 hours in culture with LIF addition after 7 days of LIF withdrawal, implying that cell proliferation rate is higher for cells receiving a sudden upregulation of genes controlling cell division through LIF addition. Although the upregulation of the genes is rapid, the downregulation of these genes through LIF withdrawal was found to take 6-7 days, while 3-4 days were required to downregulate the pou5f gene (which controls Oct4 expression). Higher concentration of LIF resulted in higher ES cell proliferation rate, but GFP⁺ expression was unaffected by
(cont.) concentration. Higher seeding density resulted in greater improvement in GFP⁺ expression for LIF⁺ culture but lower non significant reduction in GFP⁺ expression in LIF⁻ culture. Low level of glucose in medium led to reduction in the rate of ES cellular mechanisms and lower Y[sub]lac/gluc (8-49 % versus 40-60 % in high glucose), but metabolic rates were consistent with cells grown in high glucose medium, implying more efficient glucose metabolism through oxidative phosphorylation. The level of serum in medium had no effect on GFP⁺ expression or cell proliferation rate in LIF⁺ cultures, but reduction in GFP⁺ expression level was higher and t[sub]d was longer in low-serum culture (71 [plus-minus] 33 hours versus 35 [plus-minus] 9 hours) in the absence of LIF.
by Yasunori Hashimura.
M.Eng.
Shi, Xinglong. "DIFFERENTIATION OF NEURAL STEM CELL USING SMALL MOLECULES IN 2D AND 3D CULTURE SYSTEM." Master's thesis, Temple University Libraries, 2015. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/363660.
Full textM.S.
The neuronal differentiation of neural stem cells (NSCs) has received much attention due to its potential for the treatment of neurodegenerative diseases (i.e., Parkinson’s and Alzheimer’s diseases). In this regard, discovering compounds that direct differentiation of NSCs is highly required to facilitate therapeutic applications. In this study, we examined various bioactive compounds (SA1, SA2, LiCl, compound B, and DHED) to induce the neuronal differentiation of human neural stem cells (hNSCs). The study was conducted on the cells grown in three dimensional (3D) hydrogel or two dimensional (2D) environment since 3D hydrogel mimics the extracellular matrix and provides physiologically more relevant environment than 2D cell culture system. Three-dimensional (3D) hydrogel systems in this study involve polysaccharides such as alginate and hyaluronic acid. Neuronal differentiation of hNSCs was monitored in genetic level and protein level by quantitative reverse transcription polymerase chain reaction (RT-qPCR) and immunocytochemistry (ICC), respectively. This study will show the effect of bioactive compounds on hNSCs differentiation in 2D and 3D culture systems.
Temple University--Theses
Takeuchi, Hiroki. "Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture." Kyoto University, 2014. http://hdl.handle.net/2433/189668.
Full textHooper, William Craig. "The characteristics of the in vivo and in vitro response of hairy cell leukemia to inducers of differentiation /." The Ohio State University, 1985. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487260531955703.
Full textMiwa, Keiko, Jong-Kook Lee, Kyoko Hidaka, Rong-qian Shi, Gen Itoh, Takayuki Morisaki, and Itsuo Kodama. "Paracrine Factors from Cultured Cardiac Cells Promote Differentiation of Embryonic Stem Cells into Cardiac Myocytes." Research Institute of Environmental Medicine, Nagoya University, 2003. http://hdl.handle.net/2237/7580.
Full textOsborn, Kay Elizabeth. "Radiation effects on human keratinocyte cultures in relation to growth factors, differentiation and stem cells." Thesis, Queen Mary, University of London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.429519.
Full textSchinzel, Robert [Verfasser]. "The culture and differentiation of human pluripotent cells into brown and white adipocytes / Robert Schinzel." Berlin : Freie Universität Berlin, 2012. http://d-nb.info/103029092X/34.
Full text