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Journal articles on the topic 'Cucumoviruses'

Author: Grafiati

Published: 4 June 2021

Last updated: 28 January 2023

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1

Haase, Anita, and Frank Rabenstein. "Serotype-specific monoclonal antibodies against two cucumoviruses: (Short communication)." Archives Of Phytopathology And Plant Protection 24, no. 2 (January 1988): 167–69. http://dx.doi.org/10.1080/03235408809437803.

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2

Dietzgen, Ralf G., Ben Callaghan, Colleen M. Higgins, Robert G. Birch, Kunrong Chen, and Zeyong Xu. "Differentiation of Peanut Seedborne Potyviruses and Cucumoviruses by RT-PCR." Plant Disease 85, no. 9 (September 2001): 989–92. http://dx.doi.org/10.1094/pdis.2001.85.9.989.

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Seedborne peanut viruses pose important constraints to peanut production and safe movement of germ plasm. They also pose a risk of accidental introduction into previously disease-free regions. We have developed reverse transcription-polymerase chain reaction (RT-PCR) assays based on identical cycling parameters which identified peanut stripe, Peanut mottle, Peanut stunt, and Cucumber mosaic viruses through production of specific DNA fragments of 234 bp, 327 bp, 390 bp, and 133 bp, respectively. Assay sensitivity in the picogram range was achieved. The two potyviruses and two cucumoviruses could be differentiated using duplex RT-PCR assays. These assays should be useful for testing peanut leaves or seeds for virus identification in epidemiological studies, seed testing or in post-entry quarantine.

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3

White, P. Scott, Francisco Morales, and Marilyn J. Roossinck. "Interspecific Reassortment of Genomic Segments in the Evolution of Cucumoviruses." Virology 207, no. 1 (February 1995): 334–37. http://dx.doi.org/10.1006/viro.1995.1088.

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4

KAMEYA-IWAKI, Mitsuro, Kimiaki MURAKAMI, Shin-ichi ITO, Kaoru HANADA, and Shuhei TANAKA. "Semipersistency of Myzus persicae Transmission of Cucumoviruses Systemically Infecting Leguminous Plants." Journal of General Plant Pathology 66, no. 1 (February 2000): 64–67. http://dx.doi.org/10.1007/pl00012922.

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5

Shi, B. J., R. H. Symons, and S. W. Ding. "In vivo expression of an overlapping gene encoded by the cucumoviruses." Journal of General Virology 78, no. 1 (January 1, 1997): 237–41. http://dx.doi.org/10.1099/0022-1317-78-1-237.

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6

FUKUMOTO, Fumiyoshi, and Hiroshi TOCHIHARA. "Similarity of the Conditions for Freeze-drying Preservation among Three Cucumoviruses." Japanese Journal of Phytopathology 58, no. 3 (1992): 366–72. http://dx.doi.org/10.3186/jjphytopath.58.366.

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7

Codoñer, Francisco M., and Santiago F. Elena. "The promiscuous evolutionary history of the family Bromoviridae." Journal of General Virology 89, no. 7 (July 1, 2008): 1739–47. http://dx.doi.org/10.1099/vir.0.2008/000166-0.

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Recombination and segment reassortment are important contributors to the standing genetic variation of RNA viruses and are often involved in the genesis of new, emerging viruses. This study explored the role played by these two processes in the evolutionary radiation of the plant virus family Bromoviridae. The evolutionary history of this family has been explored previously using standard molecular phylogenetic methods, but incongruences have been found among the trees inferred from different gene sequences. This would not be surprising if RNA exchange was a common event, as it is well known that recombination and reassortment of genomes are poorly described by standard phylogenetic methods. In an attempt to reconcile these discrepancies, this study first explored the extent of segment reassortment and found that it was common at the origin of the bromoviruses and cucumoviruses and at least at the origin of alfalfa mosaic virus, American plum line pattern virus and citrus leaf rugose virus. Secondly, recombination analyses were performed on each of the three genomic RNAs and it was found that recombination was very common in members of the genera Bromovirus, Cucumovirus and Ilarvirus. Several cases of recombination involving species from different genera were also identified. Finally, a phylogenetic network was constructed reflecting these genetic exchanges. The network confirmed the taxonomic status of the different genera within the family, despite the phylogenetic noise introduced by genetic exchange.

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8

Pacios, Luis F., and Fernando García-Arenal. "Comparison of properties of particles of Cucumber mosaic virus and Tomato aspermy virus based on the analysis of molecular surfaces of capsids." Journal of General Virology 87, no. 7 (July 1, 2006): 2073–83. http://dx.doi.org/10.1099/vir.0.81621-0.

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The plant RNA viruses Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV) (genus Cucumovirus) have similar icosahedral particles, the crystal structures of which have been reported recently. Similarity in particle structure agrees with reports of stable capsids assembled from their capsid proteins and of viable recombinant viruses with chimeric capsid proteins derived from CMV and TAV. However, differences between the cucumoviruses have been reported for physicochemical properties. Here, structural and electrostatic features of the molecular surfaces are studied to investigate their relationship with these observations. Two coat-protein recombinants with structures modelled by taking CMV and TAV as templates were also included in the analysis. Results show that there exists an external region of negative electrostatic potential that has arisen from strictly conserved charged residues situated near the external HI loop of the subunits in the capsomers. This negative domain surrounds the fivefold and quasi-sixfold axes and locates above regions of positive potential that extend to cover, nearly homogeneously, the inner surface of capsids, where interaction with encapsidated RNA occurs. Differences between the outer electrostatic distributions in CMV and TAV explain the distinct response of both viruses to variations in physicochemical conditions required for particle stability and are essential to rationalize the biological activity of the coat-protein recombinants, in spite of their seemingly distinct electrostatic characteristics.

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9

Salánki, Katalin, Ákos Gellért, Emese Huppert, Gábor Náray-Szabó, and Ervin Balázs. "Compatibility of the movement protein and the coat protein of cucumoviruses is required for cell-to-cell movement." Journal of General Virology 85, no. 4 (April 1, 2004): 1039–48. http://dx.doi.org/10.1099/vir.0.19687-0.

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For the cell-to-cell movement of cucumoviruses both the movement protein (MP) and the coat protein (CP) are required. These are not reversibly exchangeable between Cucumber mosaic virus (CMV) and Tomato aspermy virus (TAV). The MP of CMV is able to function with the TAV CP (chimera RT), but TAV MP is unable to promote the cell-to-cell movement in the presence of CMV CP (chimera TR). To gain further insight into the non-infectious nature of the TR recombinant, RNA 3 chimeras were constructed with recombinant MPs and CPs. The chimeric MP and one of the CP recombinants were infectious. The other recombinant CP enabled virus movement only after the introduction of two point mutations (Glu→Lys and Lys→Arg at aa 62 and 65, respectively). The mutations served to correct the CP surface electrostatic potential that was altered by the recombination. The infectivity of the TR virus on different test plants was restored by replacing the sequence encoding the C-terminal 29 aa of the MP with the corresponding sequence of the CMV MP gene or by exchanging the sequence encoding the C-terminal 15 aa of the CP with the same region of TAV. The analysis of the recombinant clones suggests a requirement for compatibility between the C-terminal 29 aa of the MP and the C-terminal two-thirds of the CP for cell-to-cell movement of cucumoviruses.

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10

Gellért, Á., K. Salánki, E. Huppert, G. Náray-Szabó, and E. Balázs. "Applied homology modelling in the study of cell-to-cell movement of cucumoviruses." Acta Crystallographica Section A Foundations of Crystallography 60, a1 (August 26, 2004): s127. http://dx.doi.org/10.1107/s0108767304097508.

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11

Bernal, J. J., E. Moriones, and F. Garcia-Arenal. "Evolutionary relationships in the cucumoviruses: nucleotide sequence of tomato aspermy virus RNA 1." Journal of General Virology 72, no. 9 (September 1, 1991): 2191–95. http://dx.doi.org/10.1099/0022-1317-72-9-2191.

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12

Aaziz, R., and M. Tepfer. "Recombination between Genomic RNAs of Two Cucumoviruses under Conditions of Minimal Selection Pressure." Virology 263, no. 2 (October 1999): 282–89. http://dx.doi.org/10.1006/viro.1999.9973.

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13

Leiser, R. M., and J. Schumacher. "DETECTION OF CUCUMBER MOSAIC AND TOMATO ASPERMY CUCUMOVIRUSES BY MEANS OF POLYMERASE CHAIN REACTION." Acta Horticulturae, no. 377 (October 1994): 221–22. http://dx.doi.org/10.17660/actahortic.1994.377.24.

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14

Fukumoto, Fumiyoshi. "Preservation of Alfalfa mosaic virus by freezing and freeze-drying and similarities to Cucumoviruses." Journal of General Plant Pathology 74, no. 2 (March 4, 2008): 164–70. http://dx.doi.org/10.1007/s10327-008-0082-8.

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15

Chen, B., J. W. Randles, and R. I. B. Francki. "Mixed-subunit capsids can be assembled in vitro with coat protein subunits from two cucumoviruses." Journal of General Virology 76, no. 4 (April 1, 1995): 971–73. http://dx.doi.org/10.1099/0022-1317-76-4-971.

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16

Sackey, S. T., and R. I. B. Francki. "Interaction of cucumoviruses in plants: persistence of mixed infections of cucumber mosaic and tomato aspermy viruses." Physiological and Molecular Plant Pathology 36, no. 5 (May 1990): 409–19. http://dx.doi.org/10.1016/0885-5765(90)90069-a.

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17

Hsu, H. T., L. Barzuna, Y. H. Hsu, W. Bliss, and K. L. Perry. "Identification and Subgrouping of Cucumber mosaic virus with Mouse Monoclonal Antibodies." Phytopathology® 90, no. 6 (June 2000): 615–20. http://dx.doi.org/10.1094/phyto.2000.90.6.615.

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Using a mixture of isolates of Cucumber mosaic virus (CMV) from subgroups I and II as immunogens, 20 mouse hybridoma cell lines secreting monoclonal antibodies were produced. A reliable method for efficient detection and accurate subgrouping of CMV isolates has been developed. Tests with 12 well-characterized strains of CMV and other cucumoviruses demonstrated the presence of epitopes that were virus and subgroup specific. Analyses of 109 accessions of CMV isolates collected from various parts of the world revealed 70% were subgroup I, with 20% identified as subgroup II. Seven isolates (6%) did not react with group-specific antibodies but did react with antibodies that recognized all CMV isolates. Differential reactions among isolates suggested a total of 10 epi-topes were recognized. The antigenic diversity among subgroup II CMVs was greater than for the subgroup I isolates, even though fewer subgroup II isolates were tested.

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18

Ram, Raja, Anupama Sharma, R. K. Singh, Daizy Chauhan, and A. A. Zaidi. "Cucumber Mosaic Virus on Asiatic Hybrid Lilies in India." Plant Disease 83, no. 1 (January 1999): 78. http://dx.doi.org/10.1094/pdis.1999.83.1.78a.

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Asiatic hybrid lilies are popular cut flowers with a range of bright colors. Of the several viruses reported from lily (2,3), cucumber mosaic virus (CMV) reduces flower quality and yield (1). Classical symptoms of CMV were observed in recently introduced plants of Asiatic hybrid lilies in Kangra Valley, Himachal Pradesh. The symptoms were mild leaf mosaic, ring spot, transient vein yellowing, occasionally with growth deformation, and flower breaking. Leaf samples from cvs. MonteNegro, Yellow Present, Apeldoorn, Toscana, Connecticut King, and Adelina were collected randomly on the basis of symptom expression. Viral-associated double-stranded RNA (dsRNA) analysis was used to analyze tissue from symptomatic and asymptomatic plants for evidence of a possible cucumovirus. dsRNA analysis resulted in a banding profile typical of that seen with cucumoviruses. There was no evidence of dsRNA in the asymptomatic tissue. Presence of CMV in the symptomatic plants was also confirmed by enzyme-linked immunosorbent assay with antiserum from Agdia (Elkhart, IN). Virus from symptomatic tissues was purified and 30 nm polyhedral, viruslike particles were observed that were subsequently tested for CMV with counter immunoelectrophoresis with antibodies of CMV-C and CMV-D (antibodies obtained from H. A. Scott, University of Arkansas) and ATCC CMV antisera PVAS 242-A. Our isolate differs from other prevalent CMV isolates of Kangra Valley, having a narrow host range and not being readily sap transmissible. However, this isolate is normally transmitted to progeny bulblets. Lack of fallow periods, continuous cropping of other CMV-susceptible bulbous crops, and occasional sprouting of uncollected lily bulblets enhance inoculum build-up. Planting of CMV-tested lilies is recommended to avoid disease losses and to reduce viral inoculum floriculture fields. This is the first report of CMV in Asiatic hybrid lilies in India. References: (1) M. P. Benettii and L. Tomassoli. Acta Hortic. 234:465, 1988. (2) P. Brierley. Phytopathology 30:250, 1940. (3) L. Tomassoli and M. P. Benettii. Adv. Hortic. Sci. 2:117, 1988.

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19

Chang, Peta-Gaye S., Wayne A. McLaughlin, and Sue A. Tolin. "Tissue blot immunoassay and direct RT-PCR of cucumoviruses and potyviruses from the same NitroPure nitrocellulose membrane." Journal of Virological Methods 171, no. 2 (February 2011): 345–51. http://dx.doi.org/10.1016/j.jviromet.2010.11.018.

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20

Shi, Bu-Jun, Peter Palukaitis, and Robert H. Symons. "Differential Virulence by Strains of Cucumber mosaic virus is Mediated by the 2b Gene." Molecular Plant-Microbe Interactions® 15, no. 9 (September 2002): 947–55. http://dx.doi.org/10.1094/mpmi.2002.15.9.947.

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The approximately 12-kDa 2b protein, encoded by all cucumoviruses, had been shown to play an important role in viral long-distance movement, hypervirulence, and suppression of post-transcriptional gene silencing. The role of the 2b gene in the hypervirulence of Cucumber mosaic virus (CMV) and whether hypervirulence was linked to movement were analyzed using a hybrid virus (CMV-qw), generated by replacing the 2b gene in a subgroup II strain, Q-CMV, with the 2b gene from a subgroup IA strain, WAII-CMV. CMV-qw was more virulent than Q-CMV or WAII-CMV on most of the host plant species tested. Northern blot and nucleotide sequence analyses demonstrated that CMV-qw was stably maintained during the course of infection and upon passage. Kinetic studies revealed that the hypervirulence induced by the hybrid virus was associated with neither increased viral RNA accumulation nor more rapid viral movement per se, suggesting that other functions of the 2b protein are important in determining the hypervirulence.

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21

Shi, Bu-Jun, Jane Miller, Robert H. Symons, and Peter Palukaitis. "The 2b Protein of Cucumoviruses Has a Role in Promoting the Cell-to-cell Movement of Pseudorecombinant Viruses." Molecular Plant-Microbe Interactions® 16, no. 3 (March 2003): 261–67. http://dx.doi.org/10.1094/mpmi.2003.16.3.261.

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Pseudorecombinant viruses (i.e., those containing a reas-sorted genome of closely related multipartite viruses) are often not as competitive as the parental viruses. The role of the 2b gene in hypervirulence and maintenance of a progressive infection was assessed in a pseudorecombinant virus formed between RNAs 1 plus 2 of Cucumber mosaic virus (CMV) and RNA 3 of Tomato aspermy virus (TAV). The presence of RNA 3 of TAV was found to affect the level of RNA accumulation but not the level of virulence. By contrast, the 2b genes of both TAV and a hypervirulent strain of CMV (WAII-CMV) were found to affect the virulence of the pseudorecombinant viruses but not the levels of viral RNA accumulation. The 2b gene rather than the overlapping open reading frame encoding the C-terminal 41 amino acids of 2a protein of the corresponding virus was found to be essential for promoting infection of the pseudorecombinant viruses in planta. However, the 2b gene was not essential for replication of pseudorecombinant viruses containing CMV RNAs 1 plus 2 and TAV RNA 3. These results indicate that the 2b protein is involved in promoting the cell-to-cell movement of the pseudorecombinant viruses. These data also suggest the existence of specific interaction between the TAV 2b protein and either RNA 3 or its encoded proteins, which may be critical for promoting or maintaining infection or both.

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22

Choi, Seung Kook, Jang Kyung Choi, Won Mok Park, and Ki Hyun Ryu. "RT–PCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers." Journal of Virological Methods 83, no. 1-2 (December 1999): 67–73. http://dx.doi.org/10.1016/s0166-0934(99)00106-8.

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23

Suzuki, Masashi, Tadaaki Hibi, and Chikara Masuta. "RNA recombination between cucumoviruses: possible role of predicted stem-loop structures and an internal subgenomic promoter-like motif." Virology 306, no. 1 (February 2003): 77–86. http://dx.doi.org/10.1016/s0042-6822(02)00050-8.

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24

Gao, Shuangyu, Jinda Lu, Xiaodong Cheng, Zhouhang Gu, Qiansheng Liao, and Zhiyou Du. "Heterologous Replicase from Cucumoviruses can Replicate Viral RNAs, but is Defective in Transcribing Subgenomic RNA4A or Facilitating Viral Movement." Viruses 10, no. 11 (October 28, 2018): 590. http://dx.doi.org/10.3390/v10110590.

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Interspecific exchange of RNA1 or RNA2 between the cucumoviruses cucumber mosaic virus (CMV) and tomato aspermy virus (TAV) was reported to be non-viable in plants previously. Here we investigated viability of the reassortants between CMV and TAV in Nicotiana benthamiana plants by Agrobacterium-mediated viral inoculation. The reassortants were composed of CMV RNA1 and TAV RNA2 plus RNA3 replicated in the inoculated leaves, while they were defective in viral systemic movement at the early stage of infection. Interestingly, the reassortant containing TAV RNA1 and CMV RNA2 and RNA3 infected plants systemically, but produced RNA4A (the RNA2 subgenome) at an undetectable level. The defect in production of RNA4A was due to the 1a protein encoded by TAV RNA1, and partially restored by replacing the C-terminus (helicase domain) in TAV 1a with that of CMV 1a. Collectively, exchange of the replicase components between CMV and TAV was acceptable for viral replication, but was defective in either directing transcription of subgenomic RNA4A or facilitating viral long-distance movement. Our finding may shed some light on evolution of subgenomic RNA4A in the family Bromoviridae.

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25

Martín-Hernández, Ana Montserrat, and Belén Picó. "Natural Resistances to Viruses in Cucurbits." Agronomy 11, no. 1 (December 24, 2020): 23. http://dx.doi.org/10.3390/agronomy11010023.

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Cucurbit viruses cause considerable economic losses worldwide. The most common viral diseases affecting this crop family are Potyviruses, Cucumoviruses, Criniviruses, Ipomoviruses, Tobamoviruses, and the emerging Begomoviruses. Four main cucurbit crops are grown worldwide, namely melon, cucumber (Cucumis), watermelon (Citrullus), and squash (Cucurbita). Huge natural variation is also available within each genus, providing valuable sources of genetic resistance to these diseases. Intraspecific and intrageneric diversity and crossability are key factors to select the optimum breeding strategies. Melon and cucumber are diverse species for which intraspecific resistance is available. Conversely, in Citrullus and Cucurbita, wild relatives provide the resistance diversity absent in watermelon and in C. pepo. Some of the classical sources used by breeders, many of which are multi-resistant, come from corresponding origin centers in Asia, Africa, and America, as well as from secondary centers of diversity. Genetic studies have identified dominant and recessive and often complex resistance. Many of the genes identified have been mapped and markers for MAS are available, but higher mapping resolutions are required to identify the corresponding genes. Only a few genes could be cloned and functionally characterized. Efforts are underway to use genome mapping and functional genomics to advance toward a genomic-assisted breeding against viral diseases in cucurbits.

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26

Thompson, Jeremy R., and Fernando García-Arenal. "The Bundle Sheath-Phloem Interface of Cucumis sativus Is a Boundary to Systemic Infection by Tomato Aspermy Virus." Molecular Plant-Microbe Interactions® 11, no. 2 (February 1998): 109–14. http://dx.doi.org/10.1094/mpmi.1998.11.2.109.

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The progress of infection of two cucumoviruses in cucumber plants was analyzed immunohistochemically. Strain Fny of cucumber mosaic virus (CMV, FFF) was found to infect cucumber tissues systemically by 6 days postinoculation (dpi), while a reassortant virus with RNAs 1+2 of Fny-CMV plus RNA3 of strain 1 of tomato aspermy virus (FFT) was unable to move long distance and infect cucumber plants systemically. FFF infection of the vasculature was detected 6 dpi in the phloem of a low percentage of both minor (order VII–VI) and major (order V–IV) veins. At 9 dpi, infection was detected in phloem cells of about 50% of both minor and major veins. FFT colonization of inoculated cotyledons followed a pathway similar to that of FFF, but virus accumulation was never detected in vascular tissues. In minor or major veins, FFT infection was arrested at the bundle sheath (BS), and at 9 dpi was not detected in intermediary or other phloem cells. Thus, our data indicate that the BS-phloem interface is a boundary for the systemic movement of these viruses in cucumber, and provide evidence of a functional difference between the plasmodesmata connecting mesophyll cells, as well as mesophyll and BS cells, which allow the movement of both FFF and FFT, from the plasmodesmata connecting BS and phloem, which allow movement of FFF but not of FFT.

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27

Shimura, Hanako, Chikara Masuta, Naoto Yoshida, Kae Sueda, and Masahiko Suzuki. "The 2b protein of Asparagus virus 2 functions as an RNA silencing suppressor against systemic silencing to prove functional synteny with related cucumoviruses." Virology 442, no. 2 (August 2013): 180–88. http://dx.doi.org/10.1016/j.virol.2013.04.015.

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28

Liu, Sijun, Xiaohua He, Gyungsoon Park, Caroline Josefsson, and Keith L. Perry. "A Conserved Capsid Protein Surface Domain of Cucumber Mosaic Virus Is Essential for Efficient Aphid Vector Transmission." Journal of Virology 76, no. 19 (October 1, 2002): 9756–62. http://dx.doi.org/10.1128/jvi.76.19.9756-9762.2002.

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ABSTRACT A prominent feature on the surfaces of virions of Cucumber mosaic virus (CMV) is a negatively charged loop structure (the βH-βI loop). Six of 8 amino acids in this capsid protein loop are highly conserved among strains of CMV and other cucumoviruses. Five of these amino acids were individually changed to alanine or lysine (an amino acid of opposite charge) to create nine mutants (the D191A, D191K, D192A, D192K, L194A, E195A, E195K, D197A, and D197K mutants). Transcripts of cDNA clones were infectious when they were mechanically inoculated onto tobacco, giving rise to symptoms of a mottle-mosaic typical of the wild-type virus (the D191A, D191K, D192A, E195A, E195K, and D197A mutants), a systemic necrosis (the D192K mutant), or an atypical chlorosis with necrotic flecking (the L194A mutant). The mutants formed virions and accumulated to wild-type levels, but eight of the nine mutants were defective in aphid vector transmission. The aspartate-to-lysine mutation at position 197 interfered with infection; the only recovered progeny (the D197K∗ mutant) harbored a second-site mutation (denoted by the asterisk) of alanine to glutamate at position 193, a proximal site in the βH-βI loop. Since the disruption of charged amino acid residues in the βH-βI loop reduces or eliminates vector transmissibility without grossly affecting infectivity or virion formation, we hypothesize that this sequence or structure has been conserved to facilitate aphid vector transmission.

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29

Damayanti, Tri Asmira, Anastasya Hondo, and Yusmani Prayogo. "Infeksi Alami Pepper Yellow Leaf Curl Virus dan Sweet potato virus C Pada Ubi Jalar di Malang, Jawa Timur." Jurnal Fitopatologi Indonesia 15, no. 6 (December 11, 2019): 248–54. http://dx.doi.org/10.14692/jfi.15.6.248-254.

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Gejala tulang daun kuning (vein yellowing) dan malformasi daun yang diduga disebabkan oleh virus ditemukan pada ubi jalar IR Melati di daerah Kendalpayak, Malang, Jawa Timur. Amplifikasi DNA/cDNA menggunakan primer universal Begomovirus, Potyvirus, dan Cucumovirus menunjukkan positif teramplifikasi DNA dengan primer universal Begomovirus, dan Potyvirus, namun negatif dengan primer universal Cucumovirus. Berdasarkan runutan sikuen nukleotida, gejala tulang daun kuning dan malformasi daun disebabkan oleh infeksi ganda Pepper yellow leaf curl virus (PYLCV) dan Sweet potato virus C (SPVC). Analisis identitas DNA dengan perangkat lunak BioEdit menunjukkan homologi paling tinggi sebesar 98.5% terhadap PYLCV isolat cabai dari Bangli Bali, dan sebesar 98% terhadap SPVC dengan isolat ubi jalar asal Jepang dan Amerika Serikat. Laporan ini merupakan temuan baru infeksi alami PYLCV dan SPVC pada ubi jalar di Indonesia.

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30

Aebig, Joan A., Kathryn Kamo, and Hei-Ti Hsu. "Biolistic inoculation of gladiolus with cucumber mosaic cucumovirus." Journal of Virological Methods 123, no. 1 (January 2005): 89–94. http://dx.doi.org/10.1016/j.jviromet.2004.09.010.

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31

Varveri and Boutsika. "Characterization of cucumber mosaic cucumovirus isolates in Greece." Plant Pathology 48, no. 1 (February 1999): 95–100. http://dx.doi.org/10.1046/j.1365-3059.1999.00308.x.

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32

Holcomb, G. E., and R. A. Valverde. "Natural Infection of Salvia uliginosa with Cucumber Mosaic Cucumovirus." HortScience 33, no. 7 (December 1998): 1215–16. http://dx.doi.org/10.21273/hortsci.33.7.1215.

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Salvia uliginosa Benth. plants, in an experimental planting of Salvia species, exhibited virus-like symptoms of chlorotic line patterns and ringspots. The suspect virus was mechanically transmitted to several experimental hosts and was identified as cucumber mosaic cucumovirus (CMV) based on dsRNA gel patterns, positive reaction with CMV antiserum, and particle morphology as observed by transmission electron microscopy.

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33

REI, F. T., M. I. E. C. HENRIQUES, F. A. LEITÃO, J. F. SERRANO, and M. F. POTES. "Immunodiagnosis of cucumber mosaic cucumovirus in different olive cultivars." EPPO Bulletin 23, no. 3 (September 1993): 501–4. http://dx.doi.org/10.1111/j.1365-2338.1993.tb01360.x.

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34

Diaz-Ruiz, J. R., M. J. Avila-Rincon, and I. Garcia-Luque. "Subcellular localization of cucumovirus-associated satellite double-stranded RNAs." Plant Science 50, no. 3 (January 1987): 239–48. http://dx.doi.org/10.1016/0168-9452(87)90079-3.

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35

Karasawa, Akira, Kazuhiro Nakaho, Tetsuji Kakutani, Yuzo Minobe, and Yoshio Ehara. "Nucleotide sequence of RNA 3 of peanut stunt cucumovirus." Virology 185, no. 1 (November 1991): 464–67. http://dx.doi.org/10.1016/0042-6822(91)90800-q.

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36

Linthorst, H. J. M., and J. M. Kaper. "Cucumovirus Satellite RNAs Cannot Replicate Autonomously in Cowpea Protoplasts." Journal of General Virology 66, no. 8 (August 1, 1985): 1839–42. http://dx.doi.org/10.1099/0022-1317-66-8-1839.

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Megahed, A. A., Kh A. El Dougdoug, B. A. Othman, S. M. Lashin, M. A. Ibrahim, and A. R. Sofy. "A New Egyptian Satellite Strain of Cucumber Mosaic Cucumovirus." International Journal of Virology 8, no. 3 (June 15, 2012): 240–57. http://dx.doi.org/10.3923/ijv.2012.240.257.

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Sanchez-Cuevas, M.-C., and S. G. P. Nameth. "Virus-associated Diseases of Double Petunia: Frequency and Distribution in Ohio Greenhouses." HortScience 37, no. 3 (June 2002): 543–46. http://dx.doi.org/10.21273/hortsci.37.3.543.

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Double petunia plants expressing virus-like symptoms were collected in greenhouses and garden centers throughout Ohio in Spring 1997 and 1998 in an effort to determine the frequency and distribution of petunia viruses present in the state. Direct antibody-sandwich and indirect enzyme-linked immunosorbent assay (ELISA) were conducted with commercial antisera made against 13 viruses, a potyvirus kit capable of detecting 80 different potyviruses, and our antiserum raised against a tobamo-like virus inducing severe mosaic in double petunia. Viral-associated double-stranded ribonucleic acid (dsRNA) analysis and light microscopy for detection of inclusion bodies were also carried out. ELISA, dsRNA analysis, and light microscopy revealed the presence of tobacco mosaic tobamovirus, an unknown tobamo-like petunia virus, tomato ringspot nepovirus, tobacco streak ilarvirus, and tobacco ringspot nepovirus. Tomato aspermy cucumovirus, tomato spotted wilt tospovirus, impatiens necrotic spot tospovirus, alfalfa mosaic virus, cucumber mosaic cucumovirus, potato virus X potexvirus, and chrysanthemum B carlavirus were not detected. No potyviruses were identified. A number of plants with virus-like symptoms tested negative for all viruses.

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Chan, Yuan-Li, Nurali Saidov, Li-Mei Lee, Fu-Hsun Kuo, Su-Ling Shih, and Lawrence Kenyon. "Survey of Viruses Infecting Tomato, Cucumber and Mung Bean in Tajikistan." Horticulturae 8, no. 6 (June 6, 2022): 505. http://dx.doi.org/10.3390/horticulturae8060505.

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Viral diseases are major constraints to tomato, cucumber and mung bean production in most areas where these crops are grown. To identify the viruses on the crops in Tajikistan, a field survey was conducted in 2019. Samples of cucumber, mung bean and tomato with virus-like symptoms were collected and the viruses present were diagnosed by RT-PCR and PCR. Across all the samples, a very high proportion of the samples were infected with viruses from the genera Cucumovirus and Potyvirus. Cucumber mosaic virus (CMV; Cucumovirus) was very common in the collected samples of the three crops. As for Potyvirus, Potato virus Y (PVY) was detected in the collected tomato samples, Zucchini yellow mosaic virus (ZYMV) was identified in the collected cucumber samples, and Bean common mosaic virus (BCMV) was detected in 53% of the mung bean samples. Over 68% of the collected samples were infected with two or more viruses, suggesting that mixed infections are common for the three crops. Due to the results that the most identified viruses for the three crops are transmitted by aphids, the management of aphids is extremely important for the production of tomato, cucumber and mung bean in Tajikistan.

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Shafie, Radwa, Aly Hamed, and Hany El-Sharkawy. "Inducing Systemic Resistance against Cucumber Mosaic Cucumovirus using Streptomyces spp." Egyptian Journal of Phytopathology 44, no. 1 (June 30, 2016): 127–42. http://dx.doi.org/10.21608/ejp.2016.91931.

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Khalid, S., T. Yasmin, and M. H. Soomro. "First report of cucumber mosaic cucumovirus in banana from Pakistan." EPPO Bulletin 29, no. 1-2 (March 1999): 207–9. http://dx.doi.org/10.1111/j.1365-2338.1999.tb00820.x.

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Perry, K. L., and R. I. B. Francki. "Insect-mediated transmission of mixed and reassorted cucumovirus genomic RNAs." Journal of General Virology 73, no. 8 (August 1, 1992): 2105–14. http://dx.doi.org/10.1099/0022-1317-73-8-2105.

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., M. Osman, Kh EI-Dougdoug ., E. T. Abd EI-Salam ., R. M. Taha ., and R. M. El-Hamid . "Histo-Cytopathological Effects Of Cucumber Mosaic Cucumovirus On Squash Leaves." International Journal of Virology 1, no. 1 (December 15, 2004): 34. http://dx.doi.org/10.3923/ijv.2005.34.34.

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Gillaspie, A. G., and S. A. Ghabrial. "First Report of Peanut Stunt Cucumovirus Naturally Infecting Desmodium sp." Plant Disease 82, no. 12 (December 1998): 1402. http://dx.doi.org/10.1094/pdis.1998.82.12.1402a.

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Plant species in the genus Desmodium (Fabaceae) are used as forage and cover crops and include a number of common weeds such as beggarweed (D. tortuosum) and beggarlice (D. intortum). Accessions of the genus are part of the plant genetic resources collection maintained at Griffin, GA. Peanut stunt cucumovirus (PSV) was detected in naturally infected plants of Desmodium sp. PI 322505 (from Brazil) in a germ plasm regeneration plot by a direct antigen coating-enzyme-linked immunosorbent assay (DAC-ELISA) with an antiserum against PSV strain ER (subgroup I) originally isolated from cowpea in Georgia. The infected plants showed mild mosaic symptoms. Indicator host studies in the greenhouse revealed symptoms characteristic of PSV on Nicotiana tabacum cv. Burley 21 (ringspots and oak leaf pattern), Chenopodium album subsp. amaranticolor (chlorotic local lesions), and Vigna unguiculata (chlorotic spots followed by systemic mild mosaic). These symptomatic indicator plants tested positive for PSV by DAC-ELISA. Greenhousegrown plants of D. incanum (kaimi-clover) and D. uncinatum (Spanish tick-clover) were inoculated with the field isolate and the plants were tested for PSV by DAC-ELISA (10 infected of 10 tested and 3 infected of 9 tested, respectively). The PSV isolate infecting Desmodium spp. was found to contain satellite RNA and it generated the predicted products in reverse transcription-polymerase chain reactions (RT-PCRs) with primers based on specific PSV-ER sequences. The RT-PCR products were confirmed by restriction-enzyme digestion (1). This is the first report of PSV naturally infecting a member of the genus Desmodium. Because some members of this genus may grow as perennial weeds near peanut, cowpea, or other host crops, this genus may serve as an alternate/overwintering host for the virus. Reference: (1) R. A. Naidu et al. Phytopathology 85:502, 1995.

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Wilson, C. R. "First Report of Cucumber Mosaic Cucumovirus on Wasabi in Australia." Plant Disease 82, no. 5 (May 1998): 590. http://dx.doi.org/10.1094/pdis.1998.82.5.590a.

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Wasabi (Wasabia japonica (Miquel) Matsum.), a native perennial of Japan and Shakhalin Island used to produce a condiment for Japanese dishes, is under commercial development in Tasmania, Australia. Plants propagated within shade houses showed systemic necrotic flecks and veinal necrosis in leaves and sunken necrotic stem lesions similar to those, reported in Japan (1), caused by cucumber mosaic cucumovirus (CMV). Necrosis progressed rapidly, resulting in death of plants transferred to or mechanically inoculated in a glasshouse (15 to 30°C) under full light. Disease progression in plants maintained in shade houses (5 to 20°C) was slower and symptoms less severe. Presence of CMV in symptomatic plants was confirmed by enzyme-linked immunosorbent assay (ELISA), using antiserum from Agdia (Elkhart, IN), and by sap transmission tests to healthy wasabi and Chenopodium quinoa plants. Incidence of CMV among plants in the shade houses was estimated at 10%. A survey of a commercial planting of 400 to 500 plants in November 1997, using ELISA, showed an incidence of 2.6% CMV-infected plants. They were concentrated at the field margins, suggesting that the inoculum originated from external sources rather than from transplants. This pathogen could have significant impact on the longevity and production of this crop in Australia. Reference: (1) S. Adachi. 1987. Wasabi cultivation. Shizuoka Pref. Agric. Exp. Sta. Pub., Shizuoka, Japan.

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ZIEGLER, A., L. TORRANCE, S. M. MACINTOSH, G. H. COWAN, and M. A. MAYO. "Cucumber Mosaic Cucumovirus Antibodies from a Synthetic Phage Display Library." Virology 214, no. 1 (December 1995): 235–38. http://dx.doi.org/10.1006/viro.1995.9935.

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Tzanetakis, I. E. "First Report of Cucumber mosaic virus in Anemone sp. in the United States." Plant Disease 93, no. 4 (April 2009): 431. http://dx.doi.org/10.1094/pdis-93-4-0431b.

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In the spring of 2008, more than a dozen, aphid-infested, anemone plants (Anemone sp.) grown at the campus of the University of Arkansas in Fayetteville showed stunting and mosaic, whereas only two were asymptomatic. Leaf homogenates from four symptomatic plants were inoculated onto Nicotiana benthamiana that became stunted and developed severe mosaic approximately 7 days postinoculation, whereas buffer-inoculated plants remained asymptomatic. Double-stranded RNA (dsRNA) extraction (4) from symptomatic anemone revealed the presence of four predominant bands of approximately 3.2, 2.9, 2.2, and 0.9 kbp, a pattern indicative of cucumovirus infection. Cucumber mosaic virus (CMV) is the only cucumovirus reported in anemone in Europe (2) and Israel (3), and for this reason, anemone and N. benthamiana plants were tested by Protein A ELISA with antisera against CMV developed by H. A. Scott. ELISA verified the presence of CMV in symptomatic anemone and inoculated N. benthamiana, while asymptomatic plants were free of the virus. Using cucumovirus degenerate primers, essentially as described by Choi et al. (1), a region of approximately 940 bases that includes the complete coat protein gene of the virus was amplified from symptomatic anemone and N. benthamiana but not asymptomatic plants of either species. This anemone isolate (GenBank Accession No. FJ375723) belongs to the IA subgroup of CMV because it shares 99% nucleotide and 100% amino acid sequence identities with the Fny isolates of the virus. To my knowledge, this is the first report of CMV infecting anemone in the United States and an important discovery for the ornamental industry since anemone is commonly grown together with several ornamental hosts of CMV in nursery and garden settings. References: (1) S. K. Choi et al. J. Virol. Methods 83:67, 1999. (2) M. Hollings. Ann. Appl. Biol. 45:44, 1957 (3) G. Loebenstein. Acta Hortic. 722:31, 2006 (4) I. E. Tzanetakis and R. R. Martin, J. Virol. Methods 149:167, 2008.

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Emad, Al Dalain, A. Bysov, O. Shevchenko, T. Shevchenko, and V. Polischuk. "Several viral diseases of Lycopersicon esculentum circulating in Ukraine." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 68, no. 3 (2014): 96–98. http://dx.doi.org/10.17721/1728_2748.2014.68.96-98.

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This paper describes detection of some typical plant viruses infecting Lycopersicon esculentum Mill. plants in Ukraine. Diagnostics using enzyme-linked immunosorbent assay (ELISA) confirmed presence of antigens of viruses belonging to Tobamovirus (PMMoV, ToMV), Cucumovirus (CMV) and Tobravirus (TRV) genera in sap of tomato plants. When studying viral diseases of tomatoes, monoinfection was shown to be prevalent. Tomato mosaic virus (ToMV) was most common.

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Burgyán, József, and Fernando García-Arenal. "Template-Independent Repair of the 3′ End of Cucumber Mosaic Virus Satellite RNA Controlled by RNAs 1 and 2 of Helper Virus." Journal of Virology 72, no. 6 (June 1, 1998): 5061–66. http://dx.doi.org/10.1128/jvi.72.6.5061-5066.1998.

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ABSTRACT RNA viruses which do not have a poly(A) tail or a tRNA-like structure for the protection of their vulnerable 3′ termini may have developed a different strategy to maintain their genome integrity. We provide evidence that deletions of up to 7 nucleotides from the 3′ terminus of cucumber mosaic cucumovirus (CMV) satellite RNA (satRNA) were repaired in planta in the presence of the helper virus (HV) CMV. Sequence comparison of 3′-end-repaired satRNA progenies, and of satRNA and HV RNA, suggested that the repair was not dependent on a viral template. The 3′ end of CMV satRNA lacking the last three cytosines was not repaired in planta in the presence of tomato aspermy cucumovirus (TAV), although TAV is an efficient helper for the replication of CMV satRNA. With use of pseudorecombinants constructed by the interchange of RNAs 1 and 2 of TAV and CMV, evidence was provided that the 3′-end repair was controlled by RNAs 1 and 2 of CMV, which encode subunits of the viral RNA replicase. These results, and the observation of short repeated sequences close to the 3′ terminus of repaired molecules, suggest that the HV replicase maintains the integrity of the satRNA genome, playing a role analogous to that of cellular telomerases.

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Kassim, N. A. "EFFECT OF SOME PLANT EXTRACT AND ANTIBIOTIC ON Cucumber Mosaic Cucumovirus." Mesopotamia Journal of Agriculture 34, no. 4 (December 28, 2006): 134–39. http://dx.doi.org/10.33899/magrj.2006.26437.

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