Dissertations / Theses on the topic 'Cucumber'
To see the other types of publications on this topic, follow the link: Cucumber.
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Cucumber.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
1
Legault, Geneviève. "Effect of kaolin on the striped cucumber beetle («Acalymma vittatum») and cucumber growth and development." Thesis, McGill University, 2007. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=18484.
Full textAbstract:
Striped cucumber beetle (SCB) is the main pest of cucurbits in northeastern America. This project examined the efficacy of kaolin clay in controlling SCB (Acalymma vittatum; Coleoptera: Chrysomelidae) in cucumbers. Field experiments compared kaolin (Surround WP), insecticide (carbaryl; Sevin XLR) and untreated controls. In 2005, mean number of beetles was lowest in the kaolin treatment. Bacterial wilt (Erwinia tracheiphila) was greater in the controls and kaolin plants had significantly higher marketable yields than the two other treatments. In 2006, SCB numbers in kaolin were similar to the other treatments. Total marketable yield was significantly higher in the insecticide than the other treatments. Single and multiple applications of kaolin had negative short term effects on gas exchange and only negligible effects on greenhouse grown plants. In behavior experiments, kaolin reduced settling and feeding damage by SCB on treated plants. Kaolin shows potential as an alternative to insecticide especially to protect seedlings and young plants.La chrysomèle rayée du concombre (CRC) est le principal insecte ravageur des cucurbitacées. L'efficacité du kaolin pour contrôler la CRC (Acalymma vittatum) a été testée dans une culture de concombres. Un essai en champ de deux ans a comparé le kaolin (Surround WP) à un insecticide (carbaryl; Sevin XLR) et un témoin non traité. En 2005, la moyenne saisonnière de CRC était inférieure dans le kaolin. Le flétrissement bactérien (Erwinia tracheiphila) était plus abondant dans le témoin et le kaolin avait des rendements vendables significativement supérieurs aux autres traitements. En 2006, la moyenne de CRC dans le kaolin était comparable aux autres traitements. Le rendement vendable était significativement supérieur dans les parcelles traitées à l'insecticide. Une ou plusieurs applications de kaolin ont eu des effets négatifs sur les échanges gazeux de la feuille à court terme mais des effets négligeables sur la croissance des plants en serre. Lors de l'étude du comportement, le kaolin a réduit la présence des CRC et les dommages sur le feuillage. Le kaolin montre un bon potentiel comme alternative aux insecticides pour protéger les jeunes plants de cucurbitacées.
2
McQuillin, Andrew. "Aspects of cucumber mosaic virus replication." Thesis, Imperial College London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321682.
Full text
3
Clark, Lee J., Fred Harper, and L. Max Thatcher. "Cucumber Variety Trial, Safford Agricultural Center, 1985." College of Agriculture, University of Arizona (Tucson, AZ), 1986. http://hdl.handle.net/10150/214113.
Full textAbstract:
Slicing cucumbers were studied as an alternative crop for farmers in the Safford valley. Four varieties were tested, with the top variety yielding more than 676 cwt per acre. More work needs to be done to determine the quality and value of the crop, but generally it appears that slicing cucumbers could be produced in the area.
4
Heinz, Sabine. "Metabolite control of gene expression in cucumber." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302132.
Full text
5
Tungadi, Trisna Dewi. "Cucumber mosaic virus modifies plant-aphid interactions." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708288.
Full text
6
Mayers, Carl Nicholas. "Cucumber mosaic virus : defence and counter-defence." Thesis, University of Cambridge, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.621673.
Full text
7
Cooper, Jessica G. "Cucurbit Downy Mildew (Pseudoperonospora cubensis): Cucumber Resistance." Thesis, Virginia Tech, 2013. http://hdl.handle.net/10919/49606.
Full textAbstract:
Pseudoperonospora cubensis (Bert. et Curt) Rost. is the causal agent of cucurbit downy mildew (CDM). It is the most damaging cucumber pathogen on the Eastern Shore of Virginia and eastern parts of the United States. Pseudoperonospora cubensis is an obligate oomycete pathogen, infecting crops within the Cucurbitaceae family. The disease is characterized by angular chlorotic lesions and a downy or felt-like appearance on the abaxial side of the leaf. Control of this pathogen includes use of resistant cucumber cultivars and costly fungicide programs. Continuous use has led to resistance to commonly used fungicides. This has become a major concern and in response, seed companies have developed cucumber cultivars which claim downy mildew resistance. This study evaluates different cucumber cultivars and assesses their level of resistance to CDM. The results indicate that an integrated management approach of reduced fungicide application and the use of resistant cultivars can suppress levels of CDM and yield a cucumber crop. Additionally, a molecular study was conducted, comparing the relative expression of genes encoding a basic PR-1 protein, a cytosolic ascorbate peroxidase protein and three resistance (R) gene proteins, in nineteen cultivars. All of the selected genes were analyzed using real-time PCR. The relative expression levels of the R-genes varied between cultivars. The basic PR-1 protein decreased expression in the majority of the cultivars, suggesting no involvement in the first twenty-four hours. Cytosolic ascorbate peroxidase relative expression levels suggest an increase in susceptible cultivars and a decrease in tolerant cultivars.Master of Science
8
Al-Harbi, Abdulaziz R. A. K. "Salinity and the growth of cucumber in hydroponics." Thesis, Imperial College London, 1991. http://hdl.handle.net/10044/1/46641.
Full text
9
Johnston, Julie Catherine. "In vitro translation of cucumber necrosis virus RNA." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/28969.
Full textAbstract:
The in vitro translation products directed by cucumber necrosis virus (CNV) RNA were analyzed in both rabbit reticulocyte lysate and wheat germ extract cell-free translation systems. In rabbit reticulocyte lysates, one major protein of ca. 33 Mr was produced. In wheat germ extracts, four proteins of ca. 41, 33, 21 and 20 Mr were produced. Hybrid-arrested translation (HART) studies using synthetic CNV antisense RNA corresponding to the entire CNV genome demonstrated that the four major proteins synthesized from CNV virion RNA in wheat germ extracts are virus-specific translation products. The genomic locations of the CNV in vitro translation products were determined using a number of experimental approaches including: (1) HART using antisense RNA corresponding to selected regions of the CNV genome; (2) in vitro translation of synthetic messenger-sense CNV transcripts; (3) immunoprecipitation of in vitro translation products with CNV polyclonal antisera and (4) in vitro translation of size-fractionated CNV virion RNA. Together, these experiments demonstrated that the ca. 33 Mr protein is derived from the 5' proximal coding region, the ca. 41 Mr protein is derived from an internal coding region, and that at least one but probably both of the ca. 20 and 21 Mr proteins are derived from the 3' terminal coding region(s) of the CNV genome. In addition, immunoprecipitation experiments provided further evidence that the ca. 41 Mr protein is the viral coat protein. The size, number, and genomic locations of the CNV in vitro translation products reported here are in agreement with those predicted from nucleotide sequence data (Rochon & Tremaine, 1989). The natural template for the expression of downstream cistrons in the CNV genome was investigated by in vitro translation of sucrose fractionated CNV virion RNA as well as in vitro translation of messenger-sense synthetic transcripts. These studies indicate that in vitro, both subgenomic and genomic-length CNV RNA molecules may act as templates for the synthesis of the ca. 41,21 and 20 Mr proteins as well as the ca. 33 Mr protein.Land and Food Systems, Faculty of
Graduate
10
Sejbl, Iva Charlotte. "Blue light-signal transduction in etiolated cucumber hypocotyls." Thesis, University of York, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316140.
Full text
11
Westwood, Jack Henry. "Cucumber mosaic virus infection and plant-aphid interactions." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608811.
Full text
12
Crosswhite, F. S. "The Bitter Wild Cucumber of the Gila River." University of Arizona (Tucson, AZ), 1986. http://hdl.handle.net/10150/609094.
Full text
13
Crawford, T. W. Jr, J. L. Stroehlein, and R. O. Kuehl. "Manganese Stresses and Mineral Nutrition of Cucumber Plants." College of Agriculture, University of Arizona (Tucson, AZ), 1987. http://hdl.handle.net/10150/221402.
Full textAbstract:
Cucumber plants in the vegetative phase of growth received deficient, sufficient, or toxic treatment of manganese (Mn) during a 15-day period beginning 43 days after germination. Deficiency and toxicity of manganese both supressed accumulation of fresh and dry weight. Stem length, number of leaves, and number of seconday meristems per plant were not significantly different among Mn treatments. Manganese-deficient plants accumulated less manganese and nitrogen but more copper and iron, and about the same amount of zinc, phosphorus, and potassium as the Mn-sufficient plants. Manganese toxicity caused cucumber plants to accumulate less copper, nitrogen, phosphorus, and potassium, but more manganese, and about the same amounts of iron and zinc as the Mn-sufficient plants.
14
Solís-Marín, Francisco Alonso. "Systematics and phylogeny of the Holothurian family Synallactidae." Thesis, University of Southampton, 2003. https://eprints.soton.ac.uk/406908/.
Full text
15
Foust-Meyer, Nathaniel. "Assessing the conservation value and food system impacts of high tunnels." Thesis, Virginia Tech, 2015. http://hdl.handle.net/10919/73911.
Full textAbstract:
There is growing demand for food grown and sold locally, but climate often limits supply. High tunnels can often overcome these limitations. In order to encourage local availability and production of specialty crops the Natural Resource Conservation Service (NRCS) has launched the Seasonal High Tunnel initiative. This cost-share program provides farmers with a high tunnel intended to encourage the availability of locally grown fresh produce. Using mixed-methods research this thesis examines the social, economic, and conservation impacts of the NRCS high tunnel program. We have run a county by county negative binomial regression of the NRCS high tunnel distribution biophysical, socio-demographic, and market driven factors. Additionally, 7 vegetable farms throughout Virginia were visited during the 2014 growing season to compare high tunnel and field grown cucumbers (Cucumis sativus) and tomatoes (Solanum lycopersicum). Additional detail about high tunnel production and food distribution was obtained with a survey of Virginia high tunnel growers. Survey respondents indicate that the majority of their high tunnel produce is sold within 100 miles of their farm. Regression results indicate that the NRCS high tunnel program is benefiting areas where the availability of local food is high, but may be neglecting areas with historically underserved communities. Our field results show that yield and the yield per pesticide application dividend were higher in high tunnel production of both cucumber and tomato. Therefore, we conclude that, high tunnels meet conservation goals different than the ones outlined by the NRCS. The NRCS high tunnel program is promoting the expansion of local food availability. However, work remains to clarify conservational benefits and to ensure that all communities have equal access to the fresh produce they provide.Master of Science
16
Burman, Alison Jane. "Molecular studies of the cucumber mosaic virus movement protein." Thesis, Imperial College London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.309317.
Full text
17
Baker, Shirley Marie. "Hemoglobin function in a burrowing sea cucumber, Paracaudina chilensis." Thesis, University of Oregon theses, Dept. of Biology, M.S., 1988, 1988. http://hdl.handle.net/1794/9524.
Full textAbstract:
viii, 49 leaves : ill. ; 29 cm
Notes Typescript
Includes vita and abstract
Thesis (M.S.)--University of Oregon, 1988
Bibliography: leaves 44-49
Another copy on microfilm is located in Archives
18
Slater, Matthew James. "The sea cucumber Australostichopus mollis: Juvenile feeding ecology and habitat." Thesis, University of Auckland, 2009. http://hdl.handle.net/2292/5833.
Full textAbstract:
The aspidochirote sea cucumber Australostichopus mollis is a commercially valuable species of research interest as a candidate for aquaculture or polyculture. Understanding juvenile habitat and feeding biology is essential to furthering aquaculture efforts and expanding knowledge of the ecology of the species. Surveying within the Mahurangi Harbour, New Zealand, revealed highly patchy distribution of juvenile A. mollis at one site within an area of high adult density. High juvenile density correlated with sediment qualities favouring epibenthic detritivorous deposit-feeding. Juvenile A. mollis showed no distinct spatial separation from adults and no association with dense macroalgae or preference for shallower depths than adults, as may be expected from studies on other sea cucumber species. Wild caught juveniles displaced to non-juvenile habitats with novel surface sediments showed rapid growth. The results indicate that the highly patchy distribution of juveniles in the harbour is not due to differences in the food quality of sediments but rather other localised effects. Juvenile A. mollis show an ability to exploit a variety of benthic sediment food sources, with growth rates comparable to other cultured species. Juvenile A. mollis exhibited positive selectivity for organic particles both in situ and in the laboratory. Juveniles exhibited an ability to distinguish between different sediment TOM levels, an ability which became limited once TOM exceeded 3%. The results reiterate the specific nutrient recycling role of deposit-feeding sea cucumbers and may help explain the patchy distribution of juvenile sea cucumbers in heterogeneous habitats. In addition the research indicates that diet homogeneity and stability will be important in development of A. mollis artificial diets while a broad range of artificial diet grain sizes will be consumed. Juvenile A. mollis fed a high rate of mussel waste diet over three months exhibited significantly higher specific growth rate (SGR) than those fed natural sediment or algal diets. The results indicate the suitability of mussel waste as a juvenile diet and show that rapid growth can be expected among juvenile sea cucumbers cultured beneath mussel farms provided sea cucumber stocking rates are managed appropriately. Bioavailability of potential artificial diet ingredients was also systematically tested for juvenile A. mollis. Apparent digestibility was moderate for common carbohydrate sources and excellent for a range of artificial protein sources including low-cost protein sources. However, growth on artificial diets was poor and diet performance may need to be improved using fermentation steps or similar before they become viable. Rapid growth and flexible feeding preferences show A. mollis to be well suited to future aquaculture and polyculture.
19
Kirshenbaum, Sheril R. "Assessment and Management of the Maine Sea Cucumber (Cucumaria frondosa)." Fogler Library, University of Maine, 2005. http://www.library.umaine.edu/theses/pdf/KirshenbaumSR2005.pdf.
Full text
20
Yang, Rongchang. "Towards genetic engineering cucumber mosaic virus (CMV) resistance in lupins." Thesis, Yang, Rongchang ORCID: 0000-0003-2563-2015 (2000) Towards genetic engineering cucumber mosaic virus (CMV) resistance in lupins. PhD thesis, Murdoch University, 2000. https://researchrepository.murdoch.edu.au/id/eprint/41568/.
Full textAbstract:
Cucumber mosaic virus (CMV) is a serious pathogen of many economically important crops. In Western Australia (WA), CMV is a serious disease of narrow-leafed lupin, Lupinus angustifolius, which is the main grain legume crop. There is no known natural resistance genes to CMV have been identified .in narrow-leafed lupin germplasm that can be transferred to new cultivars using classical breeding techniques. The aim of this project was to develop a series of molecular resistance constructs and to apply them to produce pathogen-derived resistance to CMV in narrow-leafed lupin. A total of nine different CMV resistance gene constructs were developed. Eight constructs were based on the movement protein (MP), coat protein (CP) and replicase (Rep) genes of the WA subgroup II CMV-LY isolate originally obtained from infected narrow-leafed lupin, and one was based on the CP gene of a WA subgroup I CMV isolate from banana.
The gene constructs were cloned into the plant binary vectors pYR2 and pART27/7 driven by promoters from subterranean clover stunt virus (pPLEX) and cauliflower mosaic virus (CaMV 35S) and transferred into Agrobacterium (strain: AGL0). The constructs were used to transform Nicotiana benthamiana and narrow-leafed lupin with Basta as the selectable agent. For N. benthamiana a total of 1,120 explants were cocultivated with A. tumefaciens containing the pART27/7 resistance gene constructs (80 explants per construct). Following selection in culture, 16 putative transformants for each construct were transferred to the glasshouse for seed production and analysis. PCR analysis of T1 plants indicated that transformation had been successfully achieved for each of the resistance gene constructs. Transgenic plants were challenged with CMV and susceptibility or resistance was analysed by symptom development and ELISA. The results showed that some transgenic N. benthamiana plants that contained the Repm1 gene (defective CMV-LY ORF2a) were resistant to CMV-LY. In twenty-two PCR positive T₁ plants, 7 showed immunity, 12 were partially resistant, and 3 were susceptible to CMV-LY infection. In contrast, the antisense defective CMV-LY RNA 2 construct (Repm2) did not give good resistance to CMV-LY. Three of 12 T₁ plants with this construct were partially resistant (or had delayed symptoms) and the other nine were susceptible. Transgenic T1 plants containing a CMV-LY MP sense gene (MP1) showed limited resistance to CMV-LY. Two of 12 plants showed partial resistance (delayed symptoms) and two exhibited a recovery phenotype. The development of disease symptoms in the susceptible plants was faster than that in other transgenic and nontransgenic plants. Plants with the MPS transgene (untranslatable CMV-LY MP gene) showed some resistance to CMV-LY. One of 11 plants was highly resistant and three were partially resistant to CMV-LY. Three different versions of CMV-LY CP gene (CPI, CP3 and CP4) were transformed into N. benthamiana and the T₁ plants were challenged with CMV-LY. The level of resistance varied in transgenic plants depending on the CP genes present. Although a limited number of transgenic plants have been tested so far, it appears that plants containing CP4-1 show more effective resistance to CMV than transgenic plants with either CPl-1 or CP3-1. This result appears to be the first example of the use of a mutated CP gene that is longer than the wild type gene product (12 additional amino acids) and confers resistance to CMV.
For narrow-leafed lupin, 12,411 explants were subjected to meristem inoculation and cocultivated with A. tumefaciens containing a replicase construct (pYRRepm1) and 3,134 explants with a movement protein construct (pYRMPS1). One hundred and sixty one independent transformants survived in vitro selection and were grafted onto compatible nontransgenic rootstocks. Fifty nine plants survived the grafting process and were transferred to the glasshouse for seed production. PCR analysis of the 59 putative transgenic lines (T0) identified 7 plants positive for the pYRRepml gene and 15 for the p YRMPS 1 gene.
The complete genomic sequence of the CMV-LY isolate was also determined. The RNA1 molecule was determined to be 3,391 nucleotides (nt) in length and is predicted to contain a 5' untranslated region (UTR) of 95 nucleotides, a single open-reading frame (ORF) of 992 amino acids and a 3' UTR of 317 nt. The RNA2 molecule is 3,038 nt long and is predicted to contain a 5' UTR of 92 nt, two ORFs of 841 and 100 amino acids (ORF2a and ORF2b, respectively) and a 3' UTR of 423 nt. The RNA3 molecule is 2,003 nt long and is predicted to contain a 5' UTR of 96 nt, two ORFs of 279 and 218 amino acids (ORF3a and ORF3b, respectively) and a 3' UTR of 322 nt. Nucleotide comparisons of RNAs1-3 indicate that the LY isolate shares between 70-78% and 98-99% homology to subgroup I and subgroup II isolates, respectively. Similarly, ORFla shares 84-85% and 99% identity, ORF2a 81-84% and 94-96% identity, ORF2b 46-56% and 95-96% identity, ORF3a 82-84% and 99% identity, and ORF3b 81-83% and 99% identity. The sequence data clearly shows that there is a high degree of nucleotide and amino acid sequence homology between the CMV-LY isolate and other CMV subgroup II strains. The sequence data confirms that the LY isolate belongs to CMV subgroup II.
21
Rai, Poonam. "Extending Automated Testing To High-level Software Requirements : A study on the feasibility of automated acceptance-testing." Thesis, Mittuniversitetet, Avdelningen för informations- och kommunikationssystem, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:miun:diva-28041.
Full textAbstract:
Automated acceptance testing is the testing of software done in higher level to test whether the system abides by the requirements desired by the business clients by the use of piece of script other than the software itself. This project is a study of the feasibility of acceptance tests written in Behavior Driven Development principle. The project includes an implementation part where automated accep- tance testing is written for Touch-point web application developed by Dewire (a software consultant company) for Telia (a telecom company) from the require- ments received from the customer (Telia). The automated acceptance testing is in Cucumber-Selenium framework which enforces Behavior Driven Development principles. The purpose of the implementation is to verify the practicability of this style of acceptance testing. From the completion of implementation, it was concluded that all the requirements from customer in real world can be converted into executable specifications and the process was not at all time-consuming or difficult for a low-experienced programmer like the author itself. The project also includes survey to measure the learnability and understandability of Gherkin- the language that Cucumber understands. The survey consist of some Gherkin exam- ples followed with questions that include making changes to the Gherkin exam- ples. Survey had 3 parts: first being easy, second medium and third most difficult. Survey also had a linear scale from 1 to 5 to rate the difficulty level for each part of the survey. 1 stood for very easy and 5 for very difficult. Time when the partic- ipants began the survey was also taken in order to calculate the total time taken by the participants to learn and answer the questions. Survey was taken by 18 of the employers of Dewire who had primary working role as one of the programmer, tester and project manager. In the result, tester and project manager were grouped as non-programmer. The survey concluded that it is very easy and quick to learn Gherkin. While the participants rated Gherkin as very easy.
22
Gambley, Rhonda Leigh. "Physiological studies in the development of a transformation system for cucumis sativus L. (cucumber)." Thesis, Queensland University of Technology, 1990. https://eprints.qut.edu.au/35959/1/35959_Gambley_1990.pdf.
Full textAbstract:
A technique is described for the production de novo
shoots of Cucumis sativus L. (cucumber) using cotyledon
explants. The shoots, which arose from adventitious buds
and not from enhanced axillary branching, are confined to
a specific region at the base of the cotyledon and can be
obtained using a variety of growth regulator combinations.
The yield of shoots can be as high as 50 per cotyledon
when the basal region of the cotyledon is pretreated in
cytokinin then cut into small pieces prior to culturing.
Cotyledons appear to be suitable explants for
transformation using A. tumefaciens as they are readily
regenerated in vitro, susceptible to kanamycin sulphate
and susceptible to infection by A. tumefaciens. The in
vitro methods presented in this work should be readily
applicable to transformation experiments.
Cucumber explants including at least part of the
cotyledon, a short section of hypocotyl and the apical
bud, are capable of producing multiple axillary buds from
the seedling apex and adventitious shoots from the
hypocotyl base in media containing a low concentration of
cytokinin. The area of intact cotyledon and the presence
or absence of the apical bud affected shoot production in
both regions. Culture in continuous darkness entirely
prevented shoot development from the explant base but had
little influence on shoot production from the apex. The
influence of apical dominance on morphogenesis and
possible involvement of endogenous growth substances.
Multiple shoot development at the base of explants, in
media containing cytokinin, was dependent upon the length
of intact hypocotyl on the explants. It is suggested that
this is related not to the ratio or concentration of
endogenous growth regulators but to different sensitivity
of different regions along the hypocotyl to growth
regulators.
23
Criswell, Adam. "Screening Cucumber (Cucumis sativus) for Resistance to Downy Mildew (Pseudoperonospora cubensis)." NCSU, 2008. http://www.lib.ncsu.edu/theses/available/etd-06272008-145848/.
Full textAbstract:
Downy mildew, a foliar disease caused by the oomycete Pseudoperonospora cubensis (Berk. and Curt.) Rostow. is one of the most destructive pathogens of cucurbits. Resistant cultivars are available but nevertheless yield losses are high in North Carolina and Poland if fungicides are not used. The first objective of this experiment was to test all available plant introduction accessions from the U.S. National Plant Germplasm System of cucumber for downy mildew resistance under field conditions. The 1289 cultigens were tested at Clinton NC, USA, and Skierniewice, Poland during 2005-2007 under natural field epidemics of the disease. Averaged over locations, eighty-one cultigens were classified as highly resistant, 130 as moderately resistant, 406 as intermediate, 408 as moderately susceptible, and 271 as highly susceptible. The 40 most resistant and 10 most susceptible cultigens from these field trials, were further evaluated in replicated field and greenhouse experiments in North Carolina, along with 22 check cultivars. Results from the retest study in North Carolina confirmed the results of the initial screening study, although the range of downy mildew ratings in the North Carolina field retest were much narrower compared with the screening results obtained in the larger study. The most resistant and most susceptible lines in the screening study were also the most resistant and most susceptible lines in the field retest. The most resistant 10 cultigens averaged over both locations were Ames 2353, Ames 2354, PI 197085, PI 197088, PI 234517, PI 321008, PI 330628, PI 432878, PI 605996 and PI 618931. These cultigens originated from India, the United States, Pakistan, P.R. China and Taiwan. Despite the identification of resistant plant introduction accessions, they were not significantly better than the most resistant cultivars currently used in either North Carolina or Poland. The most positive aspect of the screening effort was that resistant plant introductions originated from diverse geographic regions. Because geographic diversity is often associated with genetic diversity in germplasm collections, the newly identified resistant typed may carry unique alleles as compared to commercial material. If so, then the potential exists to develop recombinant types from crosses of commercial by exotic materials which may be more resistant. The second objective of this study was to measure the correlation of four response traits. A low correlation among the four response traits on a diverse array of cucumber cultigens would suggest that the traits are controlled by different genes. Field studies were conducted to measure the response traits of plant stunting, leaf necrosis, chlorosis and sporulation caused by downy mildew infection. Each of the four traits were measured on 67 diverse cucumber cultigens in North Carolina and India. All cucumber cultigens were tested in four replications and two locations under natural field epidemics of the disease. A significant genotype by location interaction was found by analysis of variance and data from the two locations were analyzed separately. In North Carolina, necrosis and chlorosis were highly correlated (r=0.90) while sporulation was moderately correlated with necrosis and chlorosis(r=0.71 and r=0.70, respectively) and not significantly correlated with stunting. Stunting was moderately correlated with necrosis and chlorosis (r=0.43 and r=0.34, respectively). In India, chlorosis and sporulation were highly correlated (r=0.97) while necrosis was moderately correlated with chlorosis, sporulation and stunting (r=0.67 and r=0.0.65 and r=0.76, respectively). Stunting was moderately correlated with chlorosis and sporulation (r=0.55 and r=0.57, respectively). Sporulation or necrosis may be controlled by a different gene(s) but another year of testing is required. Stunting may also be controlled by a different gene(s) but difficulties in differentiating between stunting resulting from genotype and stunting resulting from disease must be resolved. Different degrees of correlation among chlorosis, necrosis and sporulation in North Carolina and India may be due to the presence of different races in the two locations. These differences may also be explained by the variable number and timing of ratings between the two locations. Availability of only one set of data for sporulation in North Carolina may have reduced the correlation between it and necrosis and chlorosis. Sporulation ratings need to be taken on a weekly basis rather than once during the last rating. Therefore, the possibility exists that chlorosis, necrosis and sporulation are response traits controlled by the same genes.
24
Lupuwana, Pumezo. "Identification and characterisation of South African strains of cucumber mosaic virus." Master's thesis, University of Cape Town, 1985. http://hdl.handle.net/11427/21901.
Full textAbstract:
Bibliography: pages 107-112.This project was then aimed at finding naturally occurring isolates of CMV, characterising them, producing much needed antisera and to use such antisera in a comparison with other well characterised strains by the use of new contemporary sensitive serological techniques.
25
Shetty, Nischit Vasanth. "Evaluation of the cucumber germplasm collection for fruit yield and quality." Raleigh, NC : North Carolina State University, 1999. http://www.lib.ncsu.edu/etd/public/etd-0261460100450/etd.pdf.
Full text
26
Afsharifar, Alireza. "Characterisation of minor RNAs associated with plants infected with cucumber mosaic virus." Title page, table of contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09pha2584.pdf.
Full textAbstract:
Bibliography: leaves 127-138. This thesis studies the minor double stranded RNAs (dsRNA) and single stranded RNAs (ssRNA) which are consistently associated with plants infected with Q strain of cucumber mosaic virus (Q-CMV). The investigations are focused on the structural elucidation of new RNAs which have been observed in single stranded and double stranded RNA profiles of Q strain of CMV.
27
Wahyuni, Wiwiek Sri. "Variation among cucumber mosaic virus (CMV) isolates and their interaction with plants." Title page, contents and summary only, 1992. http://web4.library.adelaide.edu.au/theses/09PH/09phw137.pdf.
Full textAbstract:
Includes appendix containing journal publications co-authored by the author. Includes bibliographical references (leaves 130-151). Eighteen strains of Cucumber mosaic virus, including forteen from Australia, two from the USA, and two from Japan were used in this study.
28
Soards, Avril Jacqueline. "The Cucumber mosaic virus 2b protein : influences on the plant-virus interaction." Thesis, University of Cambridge, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.619971.
Full text
29
Ziebell, Heiko. "Investigating the mechanism(s) underlying cross-protection of cucumber mosaic virus strains." Thesis, University of Cambridge, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.613299.
Full text
30
Roldán, Gustavo Alberto Quesada. "Supplemental LED interlighting on the physiological response and yield of mini-cucumber." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/11/11136/tde-22032018-131951/.
Full textAbstract:
On the protected horticultural industry, the artificial lighting is a common practice under reduced natural light radiation or self-shading of the leaves at lower canopies. Those conditions affect the photosynthetic activity and the yield is limited. In the last decade, the use of lamps with LED technology for supplemental lighting has increased on high-latitude countries. LED lights are more energy efficient and have lower power consumption. On these lamps is possible to regulate the intensity (quantity) and the radiation (quality) on the ideal range of light spectrum in order to convert the luminous energy into chemical energy. Not reports of use of this technology were found at the tropics. Thus, the objective of this work was to evaluate physiological parameters related with the photosynthetic activity and the yield on mini-cucumber plants grown on protected environment with supplemental LED interlighting. At Piracicaba (Sao Paulo), Brazil, three experiments were conducted on LED interlighting equipped greenhouse, two at winter seasons (2015 and 2016 years) and one at summer season (2017 year) using mini-cucumber hybrid Larino. Through the three stands, was verified an increase on the photosynthesis in the leaves and the commercial productivity of the mini-cucumber due to the LED lighting use. This increase on the commercial yield ranged between 13 and 30%, being higher the effect on the summer season. At the first stand, in addition to the light effect, grafted plants were tested. On this case, the photosynthetic rate increased with grafted plants when treated with LEDs. However, the yield did not increase with grafting. Postharvest parameters as titratable acidity, total soluble solids and long shelf life did not increase as consequence of supplemental LED lighting. Studying the light response curves, it was verified that above a photosynthetic photon flux density of 400 μmol m-2s-1, plants treated with supplemental lighting showed higher values of net CO2 assimilation. The light compensation point was also higher on plants with LEDs. In the case of the CO2 response curve, plants treated with LED lighting verified higher increase on the CO2 assimilation rate when the plant internal CO2 concentration increased. Parameters like Rubisco carboxylase activity, rate of electron transport and leaf dark respiration were also higher on plants under supplemental LED lighting. The lower consumption of electricity and the yield increase on the summer stand allowed registering greater energy and light efficiency on this cycle when compared with the winter stands. For the same reason, the cost analysis revealed that only at the summer stand the LED interlighting was profitable. At that time, the total net income was positive and the benefit cost ratio favorable (1.15).Na produção hortícola em ambiente protegido, o emprego de luz artificial é uma prática comum, quando condições de radiação natural reduzidas ou o autosombreamento das folhas nos estratos médio e inferior do dossel prejudicam a atividade fotossintética da planta, e consequentemente, limitam a produtividade. Na última década, em países de alta latitude, tem aumentado o uso de lâmpadas com tecnologia de luz LED para fazer essa suplementação luminosa. As luzes do tipo LED são mais eficientes energeticamente e apresentam baixo consumo de energia. Também nessas lâmpadas é possível regular a intensidade (quantidade) e a radiação (qualidade) na faixa ideal do espectro de luz visível para a conversão da energia luminosa em energia química. Em países de clima tropical, experiências dessa natureza não estão reportadas. Assim, foi realizado este trabalho com o objetivo de avaliar parâmetros fisiológicos relacionados com a atividade fotossintética e a produtividade de minipepino cultivado em um ambiente protegido com suplementação luminosa de tipo LED. Em Piracicaba (SP), foram conduzidos três experimentos em casa de vegetação, sendo realizados no inverno (anos 2015 e 2016) e no verão (ano 2017), usando plantas de minipepino híbrido Larino. Foi constatado nos três ciclos aumento na fotossíntese da planta e na produção de pepino comercial devido ao emprego da luz LED. Esse aumento no rendimento comercial variou entre 13 e 30%, sendo maior o efeito no ciclo do verão. No primeiro ciclo além do efeito da luz LED, plantas enxertadas foram testadas. Nesse caso, as plantas enxertadas mostraram também aumento na taxa fotossintética quando submetidas à suplementação luminosa, porém a produtividade não aumentou com a enxertia. Parâmetros de pós-colheita como acidez titulável, teor de sólidos solúveis e vida de prateleira não aumentaram pela suplementação com LED. Estudando as curvas de resposta à luz foi constatado que acima de 400 μmol m-2s-1 na densidade do fluxo de fótons, as plantas com suplementação luminosa mostraram valores maiores na taxa de assimilação líquida de CO2. O ponto de compensação à luz também foi superior em plantas com LED. No caso da curva de resposta ao CO2, as plantas tratadas com luz LED mostraram maior incremento na taxa de assimilação de CO2 quando a concentração interna de CO2 na planta aumentou. Parâmetros como atividade da Rubisco carboxilase, taxa de transporte de elétrons e a respiração obscura foram também maiores em plantas sob luz LED. O menor consumo de eletricidade junto com o aumento na produtividade no ciclo de verão favoreceu maior eficiência no uso da energia e da luz nesse ciclo, quando comparado com os ciclos de inverno. Por esse mesmo motivo, a análise de custos revelou que a suplementação luminosa com luz LED somente foi benéfica no ciclo de verão. Nessa época, a renda líquida total foi positiva e a relação custo beneficio favorável (1.15).
31
Mujer, Cesar V. "Purification and characterization of endo-xylanase and [beta]-xylosidase from cucumber seeds /." The Ohio State University, 1989. http://rave.ohiolink.edu/etdc/view?acc_num=osu148767568717363.
Full text
32
Aroonrungshikul, Chuanpis. "Physiological and Biochemical Studies on the Seed Dormancy of Local Thai Cucumber." Kyoto University, 2002. http://hdl.handle.net/2433/150373.
Full text
33
Balcı, Evrim Doğanlar Sami. "Genetic characterization of cucumber mosaic virus(CMV)resistance in tomato and pepper." [s.l.]: [s.n.], 2005. http://library.iyte.edu.tr/tezler/master/biyoloji/T000388.pdf.
Full text
34
PASQUINI, VIVIANA. "Reproductive biology and ecology of the sea cucumber Holothuria tubulosa Gmelin, 1788." Doctoral thesis, Università degli Studi di Cagliari, 2022. http://hdl.handle.net/11584/331327.
Full textAbstract:
Sea cucumbers play a key role on the benthic ecosystem functioning and marine biodiversity. They bioturbate the seafloor sediment reducing the organic load, recycling nutrients and redistributing surface sediments assuming the role of keystone species in the bioremediation process in marine ecosystems. Many sea cucumbers have also a remarkable food interest particularly in Asia as traditional food product and object of traditional folk medicine, a food market resulting in an international demand. The increasing of uncontrolled international harvesting led to the overexploitation of many holothurians species, including the Mediterranean sea cucumber Holothuria tubulosa, the species I focused on in the present PhD thesis. The Italian government in 2018, adopting a precautionary approach, declared a moratorium on all fishing of sea cucumbers with the intention to understand the biological characteristics and the available stock, although this latter objective remained only theoretical. The Mediterranean sea cucumber H. tubulosa, although one of the most common sea cucumber species, showed a knowledge gap in many research areas starting from its life history traits, those characteristics that play a crucial role in the species management and assessment. A careful preliminary review allowed me to focus on some important questions to face the gaps in the life history, biology and ecology of H. tubulosa that then resulted in the structuring of the doctoral project. My key questions were: When and How does this species spawn in the central Mediterranean? Which are the environmental cues that lead the spawning? Can the early larval stages be affected by density-dependent factors? Moreover, what are the stimuli related to food availability affecting settlement and metamorphosis of larvae of H. tubulosa? Finally, I dealt with the important issue of the feeding behavior of adults of this deposit feeder species. My last question regarded the ecological role of H. tubulosa and its effect on the sedimentary organic matter, in particular: Can H. tubulosa modify the trophic status of the marine sediment? Can H. tubulosa select the sediment particles during the food ingestion and can it concentrate the protein-rich particles in the digestive tract?
I sought to answer all these issues with different ecological approaches, by using experimental manipulations that have been coupled with documentation of natural processes, and field work, that required the development of complex sampling and experimental designs and evaluation in the field of experimental and statistical procedures. Four developments have followed from these research lines. First, H. tubulosa showed a unique reproductive event during the year in the central Mediterranean, corresponding to a relatively short time interval during the end of the Summer season. Second, the most effective trigger for spawning of H. tubulosa was the bubbling, which acted in the increasing of the water turbulence. The third development is that the algal biofilm that allowed the maximum settlement success was the green algae U. lens, also ensuring high survival of the post-settlers, a crucial issue that can help the feasibility of sea cucumbers aquaculture. The fourth development shed light on the trophodinamyc of H. tubulosa, revealing that this sea cucumber can select the protein-rich particles during the feeding action and can modify, locally and temporally, the trophic status of the sediment by producing feces rich in the most labile components of the organic matter.
35
Livieratos, Ioannis. "Molecular studies on cucurbit yellow stunting disorder virus." Thesis, Imperial College London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312754.
Full text
36
Guiu, Aragonés Cèlia. "Study of Cucumber mosaic virus infection in the resistant melon accession PI 161375." Doctoral thesis, Universitat Autònoma de Barcelona, 2014. http://hdl.handle.net/10803/284908.
Full textAbstract:
L’accessió exòtica de meló PI 161375 presenta una barreja de resistència qualitativa i quantitativa front a la infecció per CMV depenent de la soca. Anteriorment s’ha descrit la presencia del gen recessiu de resistència cmv1 situat en el grup de lligament XII, i que conferia resistència total només a algunes soques de CMV (Essafi et al., 2009). En aquesta tesi hem ampliat els coneixements sobre la resistència determinada pel gen cmv1 present en meló i hem obtingut la seqüència i els clons infectius de la soca M6. Aquesta tesi ha estat estructurada en tres capítols.
En el primer capítol vam analitzar la resistència conferida pel gen cmv1 en 11 soques de CMV del subgrup I i II. Els resultats van indicar que cmv1 conferia resistència total a les soques del subgrup II però no a les del subgrup I. Mitjançant l’ús dels clons infectius de les soques CMV-LS (subgrup II) i CMV-FNY (subgrup I) vam fer combinacions entre els RNAs d’ambdues soques podent localitzar el determinant de virulència en el RNA3. Virus quimèrics entre FNY i LS van indicar-nos que el determinant de virulència estava en els 209 aminoàcids de l’extrem N-terminal de la proteïna de moviment. Mitjançant mutagènesi dirigida vàrem identificar una combinació de 4 posicions específiques que confereixen a LS l’habilitat de sobrepassar la resistència conferida pel gen cmv1 quan els substituïm pels residus corresponents de la soca FNY.
El segon capítol tracta de la caracterització de la resistència conferida pel gen cmv1. La soca CMV-LS és capaç de replicar-se i de moure’s cèl·lula a cèl·lula en la fulla inoculada de la línia resistent. No obstant, LS és incapaç d’envair el floema ja que no hem pogut detectar virus en el floema de la línia resistent. Mitjançant immunomarcatge de CMV amb or col·loïdal hem identificat el límit entre cèl·lules de la beina (BS) i parènquima vascular (VP) o cèl·lules acompanyants (IC) com a barrera que impedeix la infecció sistèmica en la línia portadora del gen cmv1. Amb els resultats obtinguts hem demostrat que la resistència determinada pel gen cmv1 interromp l’entrada del virus al sistema vascular, impedint així una infecció sistèmica.
En el tercer capítol vam obtenir la seqüència de la soca CMV-M6 i vam generar clons moleculars capaços d’infectar sistèmicament N. benthamiana i meló.La accesión exótica de melón PI 161375 presenta una mezcla de resistencia cualitativa y cuantitativa frente a la infección por CMV, dependiendo de la cepa. Anteriormente se describió en nuestro laboratorio la presencia del gen recesivo de resistencia cmv1 situado en el grupo de ligamiento XII, y que confería resistencia total sólo a algunas cepas de CMV (Essafi et al., 2009). En esta tesis hemos ampliado los conocimientos sobre la resistencia mediada por el gen cmv1 presente en melón y hemos obtenido la secuencia y los clones infectivos de la cepa M6. La tesis ha sido estructurada en tres capítulos. En el primer capítulo analizamos la resistencia conferida por el gen cmv1 en 11 cepas de CMV del subgrupo I y II. Los resultados indicaron que cmv1 confería resistencia total a las cepas del subgrupo II pero no a las del subgrupo I. Mediante el uso de los clones infecciosos de las cepas CMV-LS (subgrupo II) y CMV-FNY (subgrupo I) hicimos combinaciones entre los RNAs de ambas cepas, pudiendo localizar el determinante de virulencia en el RNA3. Quimeras entre FNY y LS indicaron que el determinante de virulencia estaba en los 209 aminoácidos del extremo N-terminal de la proteína de movimiento (MP). Mediante mutagénesis dirigida identificamos una combinación de 4 posiciones específicas que confieren a LS la habilidad de sobrepasar la resistencia mediada por cmv1 cuando las sustituimos por los residuos correspondientes de la cepa FNY. El segundo capítulo trata de la caracterización de la resistencia conferida por el gen cmv1. La cepa CMV-LS es capaz de replicarse y moverse célula a célula en la hoja inoculada de la línea resistente. No obstante, LS es incapaz de invadir el floema ya que no hemos podido detectar virus en el floema de la línea resistente. Mediante inmunomarcaje de CMV con oro coloidal hemos identificado el límite entre células de la vaina (BS) y parénquima vascular (VP) o células acompañantes (IC) como barrera que impide la infección sistémica en la línea portadora del gen cmv1. Con los resultados obtenidos hemos demostrado que la resistencia determinada por el gen cmv1 interrumpe la entrada del virus al sistema vascular, impidiendo así una infección sistémica. En el tercer capítulo hemos obtenido la secuencia de la cepa CMV-M6 y generado clones moleculares capaces de infectar sistémicamente N. benthamiana y melón.
The exotic melon accession PI 161375 shows a complex mixture of qualitative and quantitative resistance to Cucumber mosaic virus (CMV) infection, depending on the strain. Previously, the presence of a recessive gene (cmv1) in the linkage group XII conferring total resistance to a set of CMV strains was reported in our laboratory (Essafi et al., 2009). In this thesis we have extended the knowledge about the cmv1-mediated resistance present in melon and have obtained the sequence of the strain CMV-M6 and its infectious clones. This thesis is divided in three chapters. In the first chapter, we have analysed the cmv1-mediated resistance in 11 strains of CMV from subgroup I and II and have established that cmv1 confers total resistance only to strains of subgroup II. Using infectious clones of strains CMV-LS (subgroup II) and CMV-FNY (subgroup I) we have made combinations between RNAs of both strains showing that the determinant of the virulence is located in RNA3. Chimaeras between CMV-FNY and CMV-LS showed that the determinant of virulence is in the N-terminal 209 amino acids of the movement protein (MP). By directed mutagenesis, we identified a combination of four specific positions that confer to LS the ability to overcome cmv1-mediated resistance when exchanged for the corresponding FNY residues. In the second chapter, we have characterized the resistance mediated by cmv1. The strain CMV-LS is able to replicate and move cell to cell in the inoculated leaf of the resistant line. However, it is not able to invade the sieve elements since it has not been detected in the phloem of the resistant line. By immunogold labelling of CMV particles we have identified that the boundary between bundle sheath cells (BS) and vascular parenchyma (VP) or intermediary cells (IC) impedes the systemic infection in the resistant line. Altogether, our results demonstrate that the resistance determined by cmv1 involves interruption of the virus entry into the vascular system and therefore, inability to develop a systemic infection. In the third chapter, we have obtained the sequence of CMV-M6 strain and generated infectious clones able to infect systemically N. benthamiana and melon.
37
Dias, Paulo Rogério Parente [UNESP]. "Caracterização de isolados e reação de Capsicum spp. ao Cucumber mosaic virus (CMV)." Universidade Estadual Paulista (UNESP), 2004. http://hdl.handle.net/11449/105424.
Full textAbstract:
Made available in DSpace on 2014-06-11T19:35:00Z (GMT). No. of bitstreams: 0
Previous issue date: 2004-11-09Bitstream added on 2014-06-13T19:05:02Z : No. of bitstreams: 1
dias_prp_dr_botfca.pdf: 1303201 bytes, checksum: ff13b531a76b1d9405f4151a52ea6f09 (MD5)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Universidade Estadual Paulista (UNESP)
Cucumber mosaic virus (CMV), uma espécie do gênero Cucumovirus, é um dos mais importantes vírus que infecta pimentão, causando prejuízos consideráveis na produção em todo o mundo. Quando da infecção precoce, em geral, ambas a qualidade e a quantidade de frutos produzidos são afetados. O vírus apresenta inúmeras estirpes capazes de infectar pimentão, diferindo na expressão dos sintomas. CMV pode infectar mais de 865 espécies de plantas, incluindo ervas daninhas, sendo transmitido por diversas espécies de afídeos de maneira não circulativa. Inseticidas são ineficazes para prevenir a disseminação da doença em virtude da forma de transmissão do vetor. No presente trabalho, verificou-se que o CMV foi o principal vírus identificado em campo. Vinte e três isolados de Capsicum spp. foram purificados biologicamente e caracterizados através de análises sorológica, biológica e molecular. Todos os 23 isolados da coleção foram classificados no subgrupo I do CMV, induzindo mosaico sistêmico, redução do desenvolvimento vegetativo e deformação foliar em Nicotiana glutinosa e Nicotiana tabacum 'Havana 425', diferindo apenas na intensidade de sintomas. Somente 8 isolados foram capazes de causar mosaico em Vigna unguiculata. Amplificação combinada com clivagem pela enzima Msp I foi eficiente para distinguir os subgrupos do CMV, resultando em banda de 500 pb somente para a amostra-controle do CMV II, dando origem a 3 fragmentos com 190, 150 e 120 pb, enquanto todos os outros isolados permaneceram com 488 pb e sem clivagem, correspondendo ao CMV-I. Não foi detectado RNA satélite em nenhum isolado do campo. A reação ao CMV de cultivares e híbridos comerciais de pimentão é desconhecida, mas tudo indica serem susceptíveis... .
Cucumber mosaic virus (CMV), a species of the genus Cucumovirus, is one of the most important virus that infect pepper, causing notable losses in pepper production worldwide. With early infection, in general, both quality and quantity of fruit produced will be affected. The virus exists as a number of strains capable of infecting pepper, differing in symptom expression. CMV can infect more than 865 plant species including many weed species and it is transmitted by many aphid species in a non-circulative manner, meaning that insecticides cannot prevent the spread of this disease. At this work, the CMV was the main virus identified in the field. Twenty-three CMV isolates from Capsicum spp. were biologically purified and characterized for serological, biological and molecular analysis. All 23 isolates from collection were found to belong to subgroup I. All isolates caused systemic mosaic, reduction of vegetative development and deformation in the leaf in N. glutinosa and N. tabacum 'Havana 425', differing in symptom intensity. Only 8 isolates were able to cause systemic mosaic in V. unguiculata. Amplification combined with Msp I cleavage was efficient to distinguish the CMV subgroups. This process resulted in a 500 pb for the CMV II control only, giving origin to three fragment with 190, 150 and 120pb, while all other isolates remained uncleaved with 488 pb, corresponding to the CMV-I isolates. It was not detect RNA satellite in a field isolates. Pepper comercial cultivars and hybrids reaction to CMV is unknowledge, but it seems to be susceptible. The identification of cultivated varieties or wild relatives of pepper that are better able to fend off attack by viral pathogens such as CMV is a critical first step towards developing resistant commercial varieties.
38
Moalemiyan, Mitra. "Optimization and evaluation of a pectin-based composite coating on mango and cucumber." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=112542.
Full textAbstract:
The current research was designed to determine the effects of different compositions of a pectin-based emulsion coating on the quality indices and shelf life extension of mango and cucumber. The fruits were treated with pectin-based coating (coated) or kept as such (control), and stored under different temperatures and relative humidities. Samples of fruits were then tested periodically to note the changes in quality as determined by visual observation, weight loss, respiration rate, color, firmness, pH, titrable acidity (TA), total soluble solids (TSS), chlorophyll content, and decay. Coated fruits displayed retarded color development, higher TA, higher chlorophyll content, greater firmness, lower pH, and lower TSS. Loss in weight and CO 2 evolution were also reduced significantly. The results of this research suggested that pectin-based coating increased the shelf life of mango and cucumber more than 100% without perceptible losses in quality.
39
Dias, Paulo Rogério Parente 1973. "Caracterização de isolados e reação de Capsicum spp. ao Cucumber mosaic virus (CMV) /." Botucatu : [s.n.], 2004. http://hdl.handle.net/11449/105424.
Full textAbstract:
Orientador: Marcelo Agenor PavanBanca: Norberto da Silva
Banca: Renate Krause Sakate
Banca: Romulo Fujito Kobori
Banca: Cyro Paulino da Costa
Resumo: Cucumber mosaic virus (CMV), uma espécie do gênero Cucumovirus, é um dos mais importantes vírus que infecta pimentão, causando prejuízos consideráveis na produção em todo o mundo. Quando da infecção precoce, em geral, ambas a qualidade e a quantidade de frutos produzidos são afetados. O vírus apresenta inúmeras estirpes capazes de infectar pimentão, diferindo na expressão dos sintomas. CMV pode infectar mais de 865 espécies de plantas, incluindo ervas daninhas, sendo transmitido por diversas espécies de afídeos de maneira não circulativa. Inseticidas são ineficazes para prevenir a disseminação da doença em virtude da forma de transmissão do vetor. No presente trabalho, verificou-se que o CMV foi o principal vírus identificado em campo. Vinte e três isolados de Capsicum spp. foram purificados biologicamente e caracterizados através de análises sorológica, biológica e molecular. Todos os 23 isolados da coleção foram classificados no subgrupo I do CMV, induzindo mosaico sistêmico, redução do desenvolvimento vegetativo e deformação foliar em Nicotiana glutinosa e Nicotiana tabacum 'Havana 425', diferindo apenas na intensidade de sintomas. Somente 8 isolados foram capazes de causar mosaico em Vigna unguiculata. Amplificação combinada com clivagem pela enzima Msp I foi eficiente para distinguir os subgrupos do CMV, resultando em banda de 500 pb somente para a amostra-controle do CMV II, dando origem a 3 fragmentos com 190, 150 e 120 pb, enquanto todos os outros isolados permaneceram com 488 pb e sem clivagem, correspondendo ao CMV-I. Não foi detectado RNA satélite em nenhum isolado do campo. A reação ao CMV de cultivares e híbridos comerciais de pimentão é desconhecida, mas tudo indica serem susceptíveis... (Resumo completo, clicar acesso eletrônico abaixo).
Abstract: Cucumber mosaic virus (CMV), a species of the genus Cucumovirus, is one of the most important virus that infect pepper, causing notable losses in pepper production worldwide. With early infection, in general, both quality and quantity of fruit produced will be affected. The virus exists as a number of strains capable of infecting pepper, differing in symptom expression. CMV can infect more than 865 plant species including many weed species and it is transmitted by many aphid species in a non-circulative manner, meaning that insecticides cannot prevent the spread of this disease. At this work, the CMV was the main virus identified in the field. Twenty-three CMV isolates from Capsicum spp. were biologically purified and characterized for serological, biological and molecular analysis. All 23 isolates from collection were found to belong to subgroup I. All isolates caused systemic mosaic, reduction of vegetative development and deformation in the leaf in N. glutinosa and N. tabacum 'Havana 425', differing in symptom intensity. Only 8 isolates were able to cause systemic mosaic in V. unguiculata. Amplification combined with Msp I cleavage was efficient to distinguish the CMV subgroups. This process resulted in a 500 pb for the CMV II control only, giving origin to three fragment with 190, 150 and 120pb, while all other isolates remained uncleaved with 488 pb, corresponding to the CMV-I isolates. It was not detect RNA satellite in a field isolates. Pepper comercial cultivars and hybrids reaction to CMV is unknowledge, but it seems to be susceptible. The identification of cultivated varieties or wild relatives of pepper that are better able to fend off attack by viral pathogens such as CMV is a critical first step towards developing resistant commercial varieties.
Doutor
40
Knox, Elizabeth. "Mixed infections of maize dwarf mosaic virus and cucumber mosaic virus in maize." Master's thesis, University of Cape Town, 1986. http://hdl.handle.net/11427/21898.
Full textAbstract:
Bibliography : pages 218-230.Maize plants collected in three geographically distinct regions of South Africa were found to be doubly infected with maize dwarf mosaic (MDMV) and cucumber mosaic virus (CMV). A mixed infection of these two viruses could be maintained in maize plants grown under laboratory conditions. The possibility of synergism or of an interference mechanism between MDMV and CMV in dual infections was investigated and it was found that prior infection with CMV interfered with subsequent infection by MDMV. MDMV and CMV were shown to be non-persistently transmitted by Myzus persicae, Rhopalosiphum padi and Rhopalosipbum maidis aphids. Protoplasts were isolated from maize seedlings and could be viably maintained for up to 66 hours. The maize protoplasts were infected with CMV and MDMV either singly, or together as a mixed inoculum. Infection curves for each virus were plotted. The presence of CMV in a mixed inoculum appeared to prevent infection of the protoplasts by MDMV. Protoplasts were isolated from plants systemically infected with CMV and/or MDMV. Superinfection of protoplasts prepared from CMV infected seedlings with MDMV was not possible. As a possible vehicle for virus infection of protoplasts liposomes were produced. Initially fluorescent dyes were incorporated in them. These were fused to the maize protoplasts. Attempts were made to encapsulate virus particles in the liposomes and fuse them to maize protoplasts but this was not successful.
41
Shimomura, Koichiro. "Quantitative trait locus analysis of agronomic traits in weedy cucumber lines for breeding." Doctoral thesis, Kyoto University, 2021. http://hdl.handle.net/2433/263362.
Full text
42
Mohammadi, Jafar. "Studies on the in vitro regeneration and micropropagation of Cucumis sativus L. (cucumber)." Thesis, University of Bath, 1990. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.237459.
Full text
43
Williams, Rhys Harold Verdon George. "Further studies on the structure and function of the cucumber mosaic virus genome : a thesis submitted to the University of Adelaide, South Australia for the degree of Doctor of Philosophy." 1988, 1988. http://web4.library.adelaide.edu.au/theses/09PH/09phw7261.pdf.
Full text
44
Gadum, Juliana [UNESP]. "Capacidade de combinação de linhagens de pepino (Cucumis sativus L.) do grupo japonês." Universidade Estadual Paulista (UNESP), 2005. http://hdl.handle.net/11449/103255.
Full textAbstract:
Made available in DSpace on 2014-06-11T19:32:26Z (GMT). No. of bitstreams: 0
Previous issue date: 2005-01-25Bitstream added on 2014-06-13T19:43:11Z : No. of bitstreams: 1
gadum_j_dr_botfca.pdf: 288776 bytes, checksum: 8e26883c5c77307d1127daa626385ae9 (MD5)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Esse trabalho teve como objetivo estimar a capacidade geral e específica de combinação, empregando-se um topcross entre linhagens e populações de pepino japonês. Foram obtidos 16 híbridos experimentais a partir de 2 populações testadoras (geração F2 de Yoshinari e de Natsusuzumi) e 8 linhagens S5 obtidas a partir do híbrido comercial Hokuho, também foi avaliado o híbrido F1 Hokuho, totalizando 27 tratamentos. O delineamento experimental utilizado foi em blocos casualizados com 4 repetições e cada parcela foi constituída de 4 plantas. Foram realizados dois experimentos em duas épocas, onde foram avaliadas as seguintes características relacionadas ao crescimento vegetativo da planta: comprimento das plantas, número de folhas, número de nós, número de brotações laterais e porcentagem de brotações. Também se avaliou o peso de frutos totais, peso de frutos comerciais, número total de frutos, número de frutos comerciais, porcentagem de frutos comerciais e peso médio de frutos comerciais. As análises de capacidades combinatória foram efetuadas de acordo com o modelo de Geraldi & Miranda Filho (1988). A população de Yoshinari (TY) apresentou, em média, melhor capacidade de se combinar com as linhagens de Hokuho. A linhagem L7 apresentou os maiores valores positivos da estimativa da CGC para a maioria das características avaliadas nos dois experimentos e os híbridos H1Y e H1N, que continham a linhagem L1 como parental, foram os que apresentaram maiores valores para a estimativa da CEC com as populações testadoras para a maioria das características avaliadas nos dois experimentos realizados, enquanto os que tinham a linhagem L5 como parental apresentaram os menores valores. Conclui-se, portanto, que há um alto potencial de se extrair linhagens de Yoshinari para cruzamentos com linhagens de Hokuho, a fim de se obter híbridos tão bons quanto o Hokuho.
The objective of the present work was obtain general and specific combining ability estimates, using a top cross between lines and japanese cucumber populations. It was obtained 16 experimental hybrids starting from 2 test populations (Yoshinari and Natsusuzumi) and 8 S5 lines obtained starting from the Hokuho commercial hybrid. The Hokuho hybrid F1 was also evaluated, totaling 27 treatments. The experimental design used was randomized block with four replications and for plant per plot. Were evaluated the following characteristics related to the vegetative growth of the plant: length of the plants, number of leaves, number of us, number of lateral produce and produce percentage. Related to the production it was evaluated: weight of total fruits, weight of commercial fruits, total number of fruits, number of commercial fruits, percentage of commercial fruits and weigh medium of commercial fruits. The analyses of combining ability were evaluated in agreement with the model of Geraldi & Miranda Filho (1988). The Yoshinari of population (TY) presented, on average, better combining ability with the lines of Hokuho, the lines L7 presented the largest positive values of gi for most of the evaluated characteristics in the two experiments and hybrids with the lines L1 it was the one that it presented larger values for CEC with the test populations for most of the evaluated characteristics in the two accomplished experiments, while the hybrids of lines L5 showed the smallest values. It is ended that there is a high potential of extracting lines of Yoshinari for crossings with lines of Hokuho, in order to obtain hybrid as good as Hokuho, with the advantage of being adapted to national conditions.
45
Chen, Baoshan. "Encapsidation of nucleic acids by cucumovirus coat proteins /." Title page, contents and summary only, 1991. http://web4.library.adelaide.edu.au/theses/09PH/09phc5183.pdf.
Full text
46
REDWAN, MIRVAT. "Physiological and genetic responses of cucumber (Cucumber sativus L.) to salt stress." Doctoral thesis, 2017. http://hdl.handle.net/2158/1080279.
Full textAbstract:
This work was divided in four chapters mainly dealt with the problem of salinity and plant physiology in particular in cucumber plants (Cucumis sativus); as one of the most severe factors that limits the yield of filed, because most of crops employed in agriculture is salt sensitive.
47
Al-Faifi, Sulieman. "Genetic analyses of mitochondrial transmission in cucumber." 2007. http://www.library.wisc.edu/databases/connect/dissertations.html.
Full text
48
Smith, Kevin P. "Biological control of Pythium diseases of cucumber." 1992. http://digital.library.wisc.edu/1793/28552.
Full textAbstract:
Thesis (M.S.)--University of Wisconsin--Madison, 1992.Typescript. Title from title screen (viewed July 3, 2008). Includes bibliographical references (p. 15-17). Online version of the print original.
49
Ho, Hsiu-Yin, and 何琇銀. "Development of transgenic watermelon plants with multiple resistance to Cucumber mosaic-、Cucumber green mottle mosaic- and Watermelon mosaic viruses." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/23150203013240623582.
Full textAbstract:
碩士中興大學
農藝學系所
95
Watermelon is an important commercial crop in Taiwan and is prone to attack by several plant viruses. Previous studies have shown that transgenic plants with multiple viral gene fragments linked to an universal silencer DNA can provide resistance to multiple virus via posttranscriptional gene silencing (PTGS). In this study, similar approach was used to develop transgenic watermelon plants resistant to three different viruses via gene silencing. Transformation constructs with selection marker gene and targer gene either in the same T-DNA or in different T-DNAs containing a silencer DNA from the partial N gene of Watermelon silver mottle virus (WSMoV) fused to the partial coat protein genes of Cucumber mosaic virus (CMV), Cucumber green mottle mosaic virus (CGMMV) and Watermelon mosaic virus (WMV) were generated and used to transform watermelon plant via Agrobacterium-mediated transformation. A total of twenty-five trnasgenic lines were generated and confirmed by PCR amplification of selection marker gene. Southern blot was used for analyzing the copy number of the target gene inserted the genome of each transgenic watermelon plant. Transgenic watermelon plants were challenged with CMV, CGMMV, WMV or a mixture of these three viruses to evaluate the multiple virus resistance. Among the twenty-five tested lines, three lines showed resistant to CGMMV and WMV. Among these three resistant lines, two were also resistant to CMV and mixed inoculation of three viruses. The result of northern blotting of transgenic watermelon plants showed a correlation between the resistant phenotype and low accumulation level of target gene RNA transcripts. Our preliminary results indicate that transgenic watermelon carrying the fusion of different viral CP gene fragments can provide multiple virus resistance. R1 seeds from the self-pollinated resistant R0 plants were obtained and will be used to evaluate the broad-spectrum resistance to CMV, CGMMV, WMV and WSMoV.
50
Turk, Farzaneh. "Characterization of factors affecting pectinmethylesterase activity in cucumber fruit." Thesis, 1989. http://hdl.handle.net/1957/27193.
Full textAbstract:
Pectinmethylesterase (PME) activity in fresh cucumber
fruit was determined. The effects of several cationic
species, temperature, pH, and acetic acid on the activity
of cucumber PME was investigated. The efficacy of
blanching, infiltration with CaCl₂ or acetic acid, and
fermentation with 0 and 40 mM CaCl₂ on inhibition of
cucumber PME activity were evaluated. Over 50% of PME
activity was present in the skin and 75% occurred within
the outer 3 mm of the fruit. Maximum stimulation of PME
occurred at 125 mM NaCl, 200 mM KCl, and 5 mM CaCl₂. Higher
levels of each cation demonstrated inhibition of PME
activity. 50% inhibition was observed at 750 mM NaCl, 800
mM KCl, and 200 mM CaCl₂. Optimum pH was 8.0 and acid pH
conditions greatly reduced activity. PME exhibited no
activity at pH 4.0. PME activity responded typically to
temperature variations with maximum activity occurring at
50°C. The temperature coefficient Q¹⁰ for PME activity between 10 and 40°C was 1.24. PME activity was slightly
stimulated by increased levels of acetic acid and reached
its maximum at 1.5% acetic acid at pH 7.5. No inhibitory
effect on PME was detected at acetic acid levels of 0 to
2.0%. Inhibition of PME by NaCl and CaCl₂ was reversible.
High levels of NaCl (1 M) stabilized PME activity while
incubation of PME activity in low levels of NaCl (0.24 M)
resulted in loss of activity over time. Both high (500 mM)
and low (5 mM) levels of CaCl₂ imparted stability to PME
activity. No detectable PME activity remained in cucumbers
after 15 days of fermentation. Addition of CaCl2 (40 mM
equilibrated) to the fermentation brine caused a rapid
reduction in PME activity during the first 6 to 12 hours
after brining. After 24 hours of brining there was no
difference in PME activity due to CaCl₂ addition. Acetic
acid infiltration at high levels (>10%) effectively reduced
the pH of skin tissue to near 4 and resulted in complete
inhibition of PME activity.
The most effective treatment for controlling cucumber
PME activity was rapid pH reduction by acetic acid
infiltration and resulting in PME inhibition. Infiltration
with very high CaCl₂ levels (>500 mM) may also be
beneficial toward accomplishing PME inhibition. Rapid
inactivation prior to brining or within 6 to 12 hours after
brining is necessary to achieve effective control of
cucumber PME activity.Graduation date: 1989