Journal articles on the topic 'CTEC'
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1
Zeng, Bo, and Chengming Luo. "Forecasting the total energy consumption in China using a new-structure grey system model." Grey Systems: Theory and Application 7, no. 2 (August 7, 2017): 194–217. http://dx.doi.org/10.1108/gs-05-2017-0011.
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Purpose China is by far the world’s largest energy consumer and importer. Reasonably forecasting the trend of China’s total energy consumption (CTEC) is of great significance. The purpose of this paper is to propose a new-structure grey system model (NSGM (1, 1)) to forecast CTEC. Design/methodology/approach Two matrices for computing the parameters of NSGM (1, 1) were defined and the specific calculation formula was derived. Since the NSGM (1, 1) model increases the number of its background values, which improves the smoothness effect of the background value and weakens the effects of extreme values in the raw sequence on the model’s performance; hence it has better simulation and prediction performances than traditional grey models. Finally, NSGM (1, 1) was used to forecast China’s total energy consumption during 2016-2025. The forecast showed CTEC will grow rapidly in the next ten years. Findings Therefore, in order to meet the target of keeping CTEC under control at 4.8 billion tons of standard coal in 2020, Chinese government needs to take necessary measures such as transforming the economic development pattern and enhancing the energy utilization efficiency. Originality/value A new-structure grey forecasting model, NSGM (1, 1), is proposed in this paper, which improves the smoothness and weakens the effects of extreme values and has a better structure and performance than those of other grey models. The authors successfully employ the new model to simulate and forecast CTEC. The research findings could aid Chinese government in formulating energy policies and help energy exporters make rational energy yield plans.
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Zhou, Yongqiang, Xiaojun Xiang, Jianping Xiong, and Changfei Gong. "Comprehensive Comparison of Progressive Optimization Algorithm Based Automatic Plan and Manually Planned Treatment Technique for Cervical-Thoracic Esophageal Cancers." Technology in Cancer Research & Treatment 19 (January 1, 2020): 153303382097328. http://dx.doi.org/10.1177/1533033820973283.
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Purpose: The purpose of the present study was first to apply the progressive optimization algorithm based automatic volumetric modulated arc therapy (POA-VMAT) technology to accelerate and improve the radiotherapy of cervicothoracic esophageal cancer (CTEC). We comprehensive analyze the feasibility, normal tissue complication probability (NTCP) and dosimetric results of POA-VMAT, manual based VMAT and step-shoot intensity-modulated radiation therapy (IMRT) plans in the treatment of CTEC. Methods: Sixty patients with CTEC with or without concomitant chemotherapy at our institution between 2017 and 2019 were retrospectively identified. The manual 7field-IMRT (7f-IMRT), Single-arc-VMAT and Double-arc-VMAT (Single-Arc/Double-Arc) plans were generated in all cases. The POA-VMAT was designed using the automatic dual-arc VMAT technology of Pinnacle3 9.10 planning system based on progressive optimization algorithm. Specially, it includes the selection of treatment techniques, the running of automated planning scripts, and the evaluation of the final radiotherapy regimen. Subsequently, quantitative evaluation of plans was performed by means of standard dose–volume histograms, homogeneity index (HI) and conformity index (CI). Results: Target dose conformity of the 7f-IMRT plan was inferior to all plans, whereas the Double-Arc plan was slightly inferior to the POA-VMAT but superior to the Single-Arc and 7f-IMRT plan. The HI for 7f-IMRT, Single-Arc, Double-Arc and POA-VMAT were 0.17 ± 0.08, 0.28 ± 0.06, 0.29 ± 0.06 and 0.28 ± 0.03, respectively. For the NTCP results, there was significant statistical difference among POA-VMAT, IMRT and VMAT plans. The total MU was reduced by 48.3% and 42.1% in Single-Arc and POA-VMAT plans compare to IMRT plans. Conclusions: By comprehensive consideration, POA-VMAT efficiently generate acceptable treatment plans for CTEC without dose escalation to OARs and overall superior to manual planning which is a good option for treating CTEC.
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Bunting, Mark D., Iain Comerford, Natalie Seach, Maree V. Hammett, Darren L. Asquith, Heinrich Körner, Richard L. Boyd, Robert J. B. Nibbs, and Shaun R. McColl. "CCX-CKR deficiency alters thymic stroma impairing thymocyte development and promoting autoimmunity." Blood 121, no. 1 (January 3, 2013): 118–28. http://dx.doi.org/10.1182/blood-2012-06-434886.
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Abstract The atypical chemokine receptor CCX-CKR regulates bioavailability of CCL19, CCL21, and CCL25, homeostatic chemokines that play crucial roles in thymic lymphopoiesis. Deletion of CCX-CKR results in accelerated experimental autoimmunity induced by immunization. Here we show that CCX-CKR deletion also increases incidence of a spontaneous Sjögren's syndrome-like pathology, characterized by lymphocytic infiltrates in salivary glands and liver of CCX-CKR−/− mice, suggestive of a defect in self-tolerance when CCX-CKR is deleted. This prompted detailed examination of the thymus in CCX-CKR−/− mice. Negatively selected mature SP cells were less abundant in CCX-CKR−/− thymi, yet expansion of both DP and immature SP cells was apparent. Deletion of CCX-CKR also profoundly reduced proportions of DN3 thymocyte precursors and caused DN2 cells to accumulate within the medulla. These effects are likely driven by alterations in thymic stroma as CCX-CKR−/− mice have fewer cTECs per thymocyte, and cTECs express the highest level of CCX-CKR in the thymus. A profound decrease in CCL25 within the thymic cortex was observed in CCX-CKR−/− thymi, likely accounting for their defects in thymocyte distribution and frequency. These findings identify a novel role for CCX-CKR in regulating cTEC biology, which promotes optimal thymocyte development and selection important for self-tolerant adaptive immunity.
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Cheunsuk, Saijai, Zhe-Xiong Lian, Guo-Xiang Yang, M. Eric Gershwin, Jeffrey R. Gruen, and Christopher L. Bowlus. "Prss16 Is Not Required for T-Cell Development." Molecular and Cellular Biology 25, no. 2 (January 15, 2005): 789–96. http://dx.doi.org/10.1128/mcb.25.2.789-796.2005.
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ABSTRACT PRSS16 is a serine protease expressed exclusively in cortical thymic epithelial cells (cTEC) of the thymus, suggesting that it plays a role in the processing of peptide antigens during the positive selection of T cells. Moreover, the human PRSS16 gene is encoded in a region near the class I major histocompatibility complex (MHC) that has been linked to type 1 diabetes mellitus susceptibility. The mouse orthologue Prss16 is conserved in genetic structure, sequence, and pattern of expression. To study the role of Prss16 in thymic development, we generated a deletion mutant of Prss16 and characterized T-lymphocyte populations and MHC class II expression on cortical thymic epithelial cells. Prss16-deficient mice develop normally, are fertile, and show normal thymic morphology, cellularity, and anatomy. The total numbers and frequencies of thymocytes and splenic T-cell populations did not differ from those of wild-type controls. Surface expression of MHC class II on cTEC was also similar in homozygous mutant and wild-type animals, and invariant chain degradation was not impaired by deletion of Prss16. These findings suggest that Prss16 is not required for quantitatively normal T-cell development.
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Carbajal-Franco, Ebzadrel, Marisol de la Fuente-Granada, Germán R. Alemán-Muench, Eduardo A. García-Zepeda, and Gloria Soldevila. "Inhibins Tune the Thymocyte Selection Process by Regulating Thymic Stromal Cell Differentiation." Journal of Immunology Research 2015 (2015): 1–15. http://dx.doi.org/10.1155/2015/837859.
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Inhibins and Activins are members of the TGF-βsuperfamily that regulate the differentiation of several cell types. These ligands were initially identified as hormones that regulate the hypothalamus-pituitary-gonadal axis; however, increasing evidence has demonstrated that they are key regulators in the immune system. We have previously demonstrated that Inhibins are the main Activin ligands expressed in the murine thymus and that they regulate thymocyte differentiation, promoting the DN3-DN4 transition and the selection of SP thymocytes. As Inhibins are mainly produced by thymic stromal cells, which also express Activin receptors and Smad proteins, we hypothesized that Inhibins might play a role in stromal cell differentiation and function. Here, we demonstrate that, in the absence of Inhibins, thymic conventional dendritic cells display reduced levels of MHC Class II (MHCII) and CD86. In addition, the ratio between cTECs and mTECs was affected, indicating that mTEC differentiation was favoured and cTEC diminished in the absence of Inhibins. These changes appeared to impact thymocyte selection leading to a decreased selection of CD4SP thymocytes and increased generation of natural regulatory T cells. These findings demonstrate that Inhibins tune the T cell selection process by regulating both thymocyte and stromal cell differentiation.
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Cheung, Winson Y., Hagen F. Kennecke, Howard John Lim, Daniel John Renouf, Sharlene Gill, Ozge Goktepe, and Caroline Speers. "A population-based analysis of outcomes in cancer patients who do not satisfy clinical trial eligibility criteria (CTEC)." Journal of Clinical Oncology 31, no. 15_suppl (May 20, 2013): 6502. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.6502.
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6502 Background: Trials have stringent inclusion and exclusion criteria to maintain internal validity. However, study findings are often applied to patients in routine practice who do not meet CTEC. Our aim was to characterize the outcomes and magnitude of treatment benefit in these patients. Methods: Patients diagnosed with stage III colon cancer from 2006 and 2008, referred to 1 of 5 regional cancer centers in British Columbia, and assessed for adjuvant chemotherapy (AC) within 12 weeks of surgery were analyzed. Patients were considered trial-eligible (TE) if aged 18 to 79 years, ECOG 0/1, CEA <10, did not receive prior chemotherapy or radiation, and had adequate blood counts and normal cardiac, liver and kidney function. All other patients were deemed trial-ineligible (TI). Results: A total of 820 patients were identified: median age was 69 years (range 60-76), 423 (52%) were men, 365 (45%) were ECOG 0/1 and 592 (72%) received AC. Among patients treated with AC, 370 (63%) were TE and 222 (37%) were TI. Compared to TI patients, those who were TE were younger (63 vs 70 years, p<0.01) and more likely to receive combination regimens rather than single agent AC (56 vs 33%, p<0.01). Outcomes were significantly different among patients who were TE, TI, and those who did not receive AC (Table). In multivariate analyses that adjusted for known prognostic factors such as age, ECOG and T and N stages, both TI patients and those not treated with AC had worse outcomes than TE patients (HR for colon cancer death 1.32, 95%CI 0.86-2.02 and 2.77, 95%CI 1.92-3.99, respectively, p trend <0.01; HR for all cause death 1.24, 95%CI 0.85-1.80 and 2.95, 95%CI 2.17-4.00, respectively, p trend <0.01). Conclusions: In this population-based cohort, colon cancer patients who did not fit CTEC were frequently treated with AC. Outcomes in this TI group were inferior to those in the TE group, but they were better than the subset that did not receive AC. Broadening CTEC to include a segment of the TI population should be considered as there appears to be benefit in selected individuals. [Table: see text]
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Cheung, Winson Y., Khodadad Rasool Javaheri, and Caroline Speers. "Outcomes of cancer patients who do not satisfy conventional clinical trial eligibility criteria (CTEC)." Journal of Clinical Oncology 31, no. 31_suppl (November 1, 2013): 128. http://dx.doi.org/10.1200/jco.2013.31.31_suppl.128.
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128 Background: Trials have stringent inclusion and exclusion criteria to maintain internal validity. However, study findings are often applied to patients in routine practice who do not meet CTEC. Our aim was to characterize the outcomes and magnitude of treatment benefit in these patients. Methods: Patients diagnosed with stage III colon cancer from 2006 and 2008, referred to 1 of 5 regional cancer centers in British Columbia, and assessed for adjuvant chemotherapy (AC) within 12 weeks of surgery were analyzed. Patients were considered trial-eligible (TE) if aged 18 to 79 years, ECOG 0/1, CEA <10, did not receive prior chemotherapy or radiation, and had adequate blood counts and normal cardiac, liver and kidney function. All other patients were deemed trial-ineligible (TI). Results: A total of 820 patients were identified: median age was 69 years (range 60-76), 423 (52%) were men, 365 (45%) were ECOG 0/1 and 592 (72%) received AC. Among patients treated with AC, 370 (63%) were TE and 222 (37%) were TI. Compared to TI patients, those who were TE were younger (63 vs. 70 years, p < 0.01) and more likely to receive combination regimens rather than single agent AC (56 vs. 33%, p < 0.01). Outcomes were significantly different among patients who were TE, TI, and those who did not receive AC (Table). In multivariate analyses that adjusted for known prognostic factors such as age, ECOG and T and N stages, both TI patients and those not treated with AC had worse outcomes than TE patients (HR for colon cancer death 1.32, 95%CI 0.86-2.02 and 2.77, 95%CI 1.92-3.99, respectively, p trend < 0.01; HR for all cause death 1.24, 95%CI 0.85-1.80 and 2.95, 95%CI 2.17-4.00, respectively, p trend < 0.01). Conclusions: In this population-based cohort, colon cancer patients who did not fit CTEC were frequently treated with AC. Outcomes in this TI group were inferior to those in the TE group, but they were better than the subset that did not receive AC. Broadening CTEC to include a segment of the TI population should be considered as there appears to be benefit in selected individuals. [Table: see text]
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Sørensen, Annette, Peter Stephensen Lübeck, Mette Lübeck, Philip Johan Teller, and Birgitte Kiær Ahring. "β-Glucosidases from a new Aspergillus species can substitute commercial β-glucosidases for saccharification of lignocellulosic biomass." Canadian Journal of Microbiology 57, no. 8 (August 2011): 638–50. http://dx.doi.org/10.1139/w11-052.
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β-Glucosidase activity plays an essential role for efficient and complete hydrolysis of lignocellulosic biomass. Direct use of fungal fermentation broths can be cost saving relative to using commercial enzymes for production of biofuels and bioproducts. Through a fungal screening program for β-glucosidase activity, strain AP (CBS 127449, Aspergillus saccharolyticus ) showed 10 times greater β-glucosidase activity than the average of all other fungi screened, with Aspergillus niger showing second greatest activity. The potential of a fermentation broth of strain AP was compared with the commercial β-glucosidase-containing enzyme preparations Novozym 188 and Cellic CTec. The fermentation broth was found to be a valid substitute for Novozym 188 in cellobiose hydrolysis. The Michaelis–Menten kinetics affinity constant as well as performance in cellobiose hydrolysis with regard to product inhibition were found to be the same for Novozym 188 and the broth of strain AP. Compared with Novozym 188, the fermentation broth had higher specific activity (11.3 U/mg total protein compared with 7.5 U/mg total protein) and also increased thermostability, identified by the thermal activity number of 66.8 vs. 63.4 °C for Novozym 188. The significant thermostability of strain AP β-glucosidases was further confirmed when compared with Cellic CTec. The β-glucosidases of strain AP were able to degrade cellodextrins with an exo-acting approach and could hydrolyse pretreated bagasse to monomeric sugars when combined with Celluclast 1.5L. The fungus therefore showed great potential as an onsite producer for β-glucosidase activity.
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Hilbig, Peter, Lukas Ibing, Benjamin Streipert, Ralf Wagner, Martin Winter, and Isidora Cekic-Laskovic. "Acetonitrile-based electrolytes for lithium-ion battery application." Current Topics in Electrochemistry 20 (December 31, 2018): 1. http://dx.doi.org/10.31300/ctec.20.2018.1-13.
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Frogley, Benjamin J., Anthony F. Hill, Richard A. Manzano, and Manab Sharma. "Bis(alkylidynyl)tellurides and ditellurides." Chemical Communications 54, no. 14 (2018): 1702–5. http://dx.doi.org/10.1039/c7cc08776d.
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The tellurocarbonylates [M(CTe)(CO)2(Tp*)]− (M = Mo, W; obtained from [M(CBr)(CO)2(Tp*)] and Li2Te or [M(CLi)(CO)2(Tp*)] and Te) react with an additional equivalent of [M(CBr)(CO)2(Tp*)] to give bis(alkylidynyl)tellurides, [M2(μ-CTeC)(CO)4(Tp*)2], whilst oxidation with [Fe(η-C5H5)2]PF6 affords the corresponding ditellurides [M2(μ-CTe2C)(CO)4(Tp*)2].
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Espinosa Torres, Mabel Del Pilar, Rigoberto Pastor Sánchez Figueredo, Arabel Moraguez Iglesias, and Alberto Carballo Peña. "El aprendizaje formativo a través de las Prácticas Profesionales de los Estudiantes de Ingeniería Mecánica." Revista Científica de FAREM-Estelí, no. 37 (March 23, 2021): 244–66. http://dx.doi.org/10.5377/farem.v0i37.11220.
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Esta investigación se ha llevando a cabo en la Facultad de Ingeniería de la Universidad de Holguín respaldado en dos Proyectos de Desarrollo Empresarial: con la Fábrica de Implementos Agrícolas “26 de Julio” y el Centro de Tecnología y Calidad Industrial (CTEC), ambos de la provincia de Holguín y toma como sustento el Curso #34: “Aprendizaje formativo y crecimiento personal”, dictado por la Doctora Raquel Bermúdez en Pedagogía 2005, y tiene la finalidad de analizar cómo el Aprendizaje Formativo aplicado a las Prácticas Profesionales en los estudiantes de Ingeniería Mecánica en la Universidad de Holguín, ubicados en las distintas entidades productivas del territorio, contribuyen a la formación profesional e integral de los estudiantes.
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Lin, Peter Ping. "Aneuploid Circulating Tumor-Derived Endothelial Cell (CTEC): A Novel Versatile Player in Tumor Neovascularization and Cancer Metastasis." Cells 9, no. 6 (June 24, 2020): 1539. http://dx.doi.org/10.3390/cells9061539.
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Hematogenous and lymphogenous cancer metastases are significantly impacted by tumor neovascularization, which predominantly consists of blood vessel-relevant angiogenesis, vasculogenesis, vasculogenic mimicry, and lymphatic vessel-related lymphangiogenesis. Among the endothelial cells that make up the lining of tumor vasculature, a majority of them are tumor-derived endothelial cells (TECs), exhibiting cytogenetic abnormalities of aneuploid chromosomes. Aneuploid TECs are generated from “cancerization of stromal endothelial cells” and “endothelialization of carcinoma cells” in the hypoxic tumor microenvironment. Both processes crucially engage the hypoxia-triggered epithelial-to-mesenchymal transition (EMT) and endothelial-to-mesenchymal transition (EndoMT). Compared to the cancerization process, endothelialization of cancer cells, which comprises the fusion of tumor cells with endothelial cells and transdifferentiation of cancer cells into TECs, is the dominant pathway. Tumor-derived endothelial cells, possessing the dual properties of cancerous malignancy and endothelial vascularization ability, are thus the endothelialized cancer cells. Circulating tumor-derived endothelial cells (CTECs) are TECs shed into the peripheral circulation. Aneuploid CD31+ CTECs, together with their counterpart CD31- circulating tumor cells (CTCs), constitute a unique pair of cellular circulating tumor biomarkers. This review discusses a proposed cascaded framework that focuses on the origins of TECs and CTECs in the hypoxic tumor microenvironment and their clinical implications for tumorigenesis, neovascularization, disease progression, and cancer metastasis. Aneuploid CTECs, harboring hybridized properties of malignancy, vascularization and motility, may serve as a unique target for developing a novel metastasis blockade cancer therapy.
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Rasool Javaheri, Khodadad, Hagen F. Kennecke, Daniel John Renouf, Howard John Lim, Tina Hsu, Caroline Speers, Ozge Goktepe, and Winson Y. Cheung. "Adjuvant treatment and outcomes in patients with stage III colon cancer (CC) who do not fit clinical trial eligibility criteria (CTEC)." Journal of Clinical Oncology 31, no. 4_suppl (February 1, 2013): 486. http://dx.doi.org/10.1200/jco.2013.31.4_suppl.486.
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486 Background: Trials have strict inclusion and exclusion criteria to maintain internal validity. However, study findings are often applied to pts in clinical practice who do not satisfy all of the CTEC. Whether these pts benefit from treatment is unclear. Our objectives were 1) to characterize cancer-specific (CSS) and overall survival (OS) in a population-based cohort of trial-eligible (TE) and trial-ineligible (TI) pts receiving adjuvant chemotherapy (CT) and 2) to compare their outcomes with those not treated with CT. Methods: Pts diagnosed with stage III CC between 2006 and 2008, referred to 1 of 5 regional cancer centers in British Columbia, and evaluated for possible adjuvant CT within 12 weeks of curative surgery were reviewed. Pts were defined as TE if aged 18 to 79 years, ECOG 0 to 1, CEA <10 ng/ml, had not received prior CT or radiation, and had adequate blood counts, cardiac, liver, and kidney function. All other pts were considered TI. Using Kaplan-Meier and Cox regression analyses, we compared outcomes between TE and TI pts who received CT vs. those who did not. Results: A total of 821 pts were identified: median age was 68 years, 52% were men, 85% were ECOG 0 to1, and 71% received adjuvant CT. Among pts treated with CT, 405 (70%) were TE and 177 (30%) were TI. Compared to TI pts, those who were TE were younger (p<0.01) and more likely to receive FOLFOX than capecitabine (65 vs. 46%, p<0.01). CSS and OS were significantly different among pts who were TE, TI, and those who did not receive CT (p<0.01) (Table). In multivariate analyses that adjusted for confounders, both TI pts and those not treated with CT had worse prognoses than TE pts (HR for CC deaths 1.32, 95%CI 0.86-2.02 and 2.77, 95%CI 1.92-3.99, respectively, p trend <0.01; HR for all deaths 1.24, 95%CI 0.85-1.80 and 2.95, 95%CI 2.17-4.00, respectively, p trend <0.01). Conclusions: A considerable number of stage III CC pts who did not fit CTEC were treated with adjuvant CT. While outcomes in the TI group were worse than those in the TE group, CSS and OS were still better than the subset that did not receive any CT. [Table: see text]
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Ivashchenko, Olha, Oleg Khudolii, and Wladyslaw Jagiello. "Strength abilities: pattern recognition method in the management of the cumulative effect of strength loads in 8-year-old boys." Pedagogy of Physical Culture and Sports 25, no. 4 (August 30, 2021): 253–60. http://dx.doi.org/10.15561/26649837.2021.0407.
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Background and Study Aim. The purpose of the study was to determine the peculiarities of using pattern recognition method in the management of the cumulative effect of strength loads in 8-year-old boys.
Materials and methods. The study participants were 48 boys aged 8. The experiment was conducted using a 22 factorial design. The study materials were processed by the IBM SPSS 22 statistical analysis program. Discriminant analysis was performed. The study examined the impact of four variants of strength load on the formation of the cumulative training effect of three, six, nine, and twelve classes in 8-year-old boys.
Results. The discriminant analysis provided information about the impact of four orthogonal variants of strength loads on the formation of the cumulative training effect of strength exercises of three, six, nine, and twelve classes in 8-year-old boys. The obtained data make it possible to choose a load mode at each step of the CTE formation and to manage schoolchildren’s strength training.
Conclusions. The verification of the obtained discriminant functions shows their high discriminative ability and value in interpretation with respect to the general population (p < 0.05). It was found that the formation of the CTE of three classes is most influenced by the third load variant, six classes – by the third load variant, nine classes – the third load variant, twelve classes – the first load variant. The discriminant function structure coefficients made it possible to identify the factor structure of the CTE of 3, 6, 9, 12 classes, to find that the CTE3, CTE6 are associated with the work at the first place “Exercises to strengthen arm muscles”, the CTE9, CTE12 – with the work at the third (“Exercises to strengthen back muscles”) and the fourth (“Exercises to strengthen leg muscles”) places. The CTE of three, six, nine, and twelve classes depends on the modes of strength exercises and has different focuses. The CTE3 – speed and strength focus; CTE6, 9 – comprehensive focus; CTE12 – explosive-strength focus. The obtained values of centroids for the CTE of 3, 6, 9, 12 classes enable the management of schoolchildren’s strength training.
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Dudakov, Jarrod A., Alan M. Hanash, Lauren F. Young, Natalie V. Singer, Mallory L. West, Robert R. Jenq, Odette M. Smith, Amanda M. Holland, Richard L. Boyd, and Marcel R. M. van den Brink. "Innate Lymphoid Cell-Derived IL-22 Mediates Endogenous Thymic Repair Under the Control of IL-23." Blood 118, no. 21 (November 18, 2011): 143. http://dx.doi.org/10.1182/blood.v118.21.143.143.
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Abstract Abstract 143 Despite being exquisitely sensitive to insult, the thymus is remarkably resilient in young healthy animals. Endogenous regeneration of the thymus is a crucial function that allows for renewal of immune competence following infection or immunodepletion caused by cytoreductive chemotherapy or radiation. However, the mechanisms governing this regeneration remain poorly understood. Thymopoiesis is a highly complex process involving cross-talk between developing thymocytes and their supporting non-hematopoietic stromal microenvironment, which includes highly specialized thymic epithelial cells (TECs) that are crucial for T cell development. IL-22 is a recently identified cytokine predominantly associated with maintenance of barrier function at mucosal surfaces. Here we demonstrate for the first time a critical role for IL-22 in endogenous thymic repair. Comparing IL-22 KO and WT mice we observed that while IL-22 deficiency was redundant for steady-state thymopoiesis, it led to a pronounced and prolonged loss of thymus cellularity following sublethal total body irradiation (SL-TBI), which included depletion of both thymocytes (p=0.0001) and TECs (p=0.003). Strikingly, absolute levels of IL-22 were markedly increased following thymic insult (p<0.0001) despite the significant depletion of thymus cellularity. This resulted in a profound increase in the production of IL-22 on a per cell basis (p<0.0001). These enhanced levels of IL-22 peaked at days 5 to 7 after SL-TBI, immediately following the nadir of thymic cellularity. This was demonstrated by a strong negative correlation between thymic cellularity and absolute levels of IL-22 (Fig 1a). In mucosal tissues the regulation of IL-22 production has been closely associated with IL-23 produced by dendritic cells (DCs) and ex vivo incubation of cells with IL-23 stimulates the production of IL-22. Following thymic insult there was a significant increase in the amount of IL-23 produced by DCs (Fig 1b) resulting in similar kinetics of intrathymic levels of IL-22 and IL-23. We identified a population of radio-resistant CD3−CD4+IL7Ra+RORg(t)+ thymic innate lymphoid cells (tILCs) that upregulate both their production of IL-22 (Fig 1c) and expression of the IL-23R (p=0.0006) upon exposure to TBI. This suggests that they are responsive to IL-23 produced by DCs in vivo following TBI and, in fact, in vitro stimulation of tILCs by IL-23 led to upregulation of Il-22 production by these cells (Fig 1d). We found expression of the IL-22Ra on cortical and medullary TECs (cTECs and mTECs, respectively), and uniform expression across both mature MHCIIhi mTEC (mTEChi) and immature MHCIIlo mTECs (mTEClo). However, in vitro stimulation of TECs with recombinant IL-22 led to enhanced TEC proliferation primarily in cTEC and mTEClo subsets (p=0.002 and 0.004 respectively). It is currently unclear if IL-22 acts as a maturation signal for mTECs, however, the uniform expression of IL-22Ra between immature mTEClo and mature Aire-expressing mTEChi, together with the preferential promotion of proliferation amongst mTEClo and cTEC seem to argue against IL-22 as a maturational signal but rather as promoter of proliferation, which ultimately leads to terminal differentiation of TECs. Of major clinical importance, administration of exogenous IL-22 led to enhanced thymic recovery (Fig. 1e) following TBI, primarily by promoting the proliferation of TECs. Consistent with this, the administration of IL-22 also led to significantly enhanced thymopoiesis following syngeneic BMT. Taken together these findings suggest that following thymic insult, and specifically the depletion of developing thymocytes, upregulation of IL-23 by DCs induces the production of IL-22 by tILCs and regeneration of the supporting microenvironment. This cascade of events ultimately leads to rejuvenation of the thymocyte pool (Fig. 1f). These studies not only reveal a novel pathway underlying endogenous thymic regeneration, but also identify a novel regenerative strategy for improving immune competence in patients whose thymus has been damaged from infection, age or cytoreductive conditioning required for successful hematopoietic stem cell transplantation. Finally, these findings may also provide an avenue of study to further understand the repair and regeneration of other epithelial tissues such as skin, lung and breast. Disclosures: No relevant conflicts of interest to declare.
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Ioelovich, Michael Yacob, and Eli Aleks Morag. "OPTIMIZATION OF PRODUCTION CONDITIONS OF AMORPHOUS CELLULOSE WITH A HIGH ENZYMATIC HYDROLYZABILITY." chemistry of plant raw material, no. 1 (March 5, 2020): 19–24. http://dx.doi.org/10.14258/jcprm.2020016579.
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In this paper, a method for producing amorphous cellulose (AC) was studied by treatment of initial cellulosic materials (MCC and mixed paper waste paper) with a cold solvent, containing 7% NaOH and 12% urea, at different solvent to cellulose (R) ratios, ml/g. Using X-ray diffraction, it was found that after treatment of MCC with the solvent at R = 3, the crystalline modification (CM) of CI of the initial cellulose is converted to CM CII, having a low crystallinity degree. In the case, where the initial cellulose is treated with the solvent at R ≥ 5, a completely amorphous cellulose (AC) is formed. Due to its high hydrolyzability, the AC with concentration of 50 g/L is converted into glucose almost completely within 48 hours under the action of the CTec-3 enzyme preparation at a dose of 30 mg/g dry substrate. The obtained AC sample can be used as an amorphous standard in the study of enzymatic hydrolysis of various types of cellulose and lignocellulose. It was found that the most beneficial enzymatic saccharification of AC is carried out at increased concentration of the cellulosic substrate, 150 g/L. Due to the high cost of MCC, it is preferable to use low-cost cellulosic raw material, such as mixed waste paper, for the industrial production of AC and glucose.
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Dai, Zheng, Heting Fu, Yufeng Zhang, Jing Zeng, Bing Tang, and Xiao-Feng Tang. "Insights into the Maturation of Hyperthermophilic Pyrolysin and the Roles of Its N-Terminal Propeptide and Long C-Terminal Extension." Applied and Environmental Microbiology 78, no. 12 (April 13, 2012): 4233–41. http://dx.doi.org/10.1128/aem.00548-12.
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ABSTRACTPyrolysin-like proteases from hyperthermophiles are characterized by large insertions and long C-terminal extensions (CTEs). However, little is known about the roles of these extra structural elements or the maturation of these enzymes. Here, the recombinant proform ofPyrococcus furiosuspyrolysin (Pls) and several N- and C-terminal deletion mutants were successfully expressed inEscherichia coli. Pls was converted to mature enzyme (mPls) at high temperatures via autoprocessing of both the N-terminal propeptide and the C-terminal portion of the long CTE, indicating that the long CTE actually consists of the C-terminal propeptide and the C-terminal extension (CTEm), which remains attached to the catalytic domain in the mature enzyme. Although the N-terminal propeptide deletion mutant PlsΔN displayed weak activity, this mutant was highly susceptible to autoproteolysis and/or thermogenic hydrolysis. The N-terminal propeptide acts as an intramolecular chaperone to assist the folding of pyrolysin into its thermostable conformation. In contrast, the C-terminal propeptide deletion mutant PlsΔC199 was converted to a mature form (mPlsΔC199), which is the same size as but less stable than mPls, suggesting that the C-terminal propeptide is not essential for folding but is important for pyrolysin hyperthermostability. Characterization of the full-length (mPls) and CTEm deletion (mPlsΔC740) mature forms demonstrated that CTEm not only confers additional stability to the enzyme but also improves its catalytic efficiency for both proteineous and small synthetic peptide substrates. Our results may provide important clues about the roles of propeptides and CTEs in the adaptation of hyperthermophilic proteases to hyperthermal environments.
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Matiku, Susan, Jethro Zuwarimwe, and Ndivhuwo Tshipala. "Community-Driven Tourism Projects’ Economic Contribution to Community Livelihoods—A Case of Makuleke Contractual Park Community Tourism Project." Sustainability 12, no. 19 (October 6, 2020): 8230. http://dx.doi.org/10.3390/su12198230.
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Tourism is a known rural economic development tool. Furthermore, sustainable tourism seeks to enhance the quality of the resident lives through its economic benefits, among others. One way of diversification economic bases in rural areas is through community-driven tourism projects (CDTP). The tourism projects provide a livelihood strategy to the communities who are involved in the projects. This study makes an attempt to build an understanding of the economic contribution of community-driven tourism projects to sustainable livelihoods. The study draws on a qualitative survey carried out in South Africa’s Makuleke contractual Park Community Project. The study utilized the community capital framework (CCF) to examine the community tourism economic capitals’ (CTEC) contribution to the community livelihoods. The study also investigated on the ripple effect of the economic capital towards other capitals within the community. Thematic content analysis was used to analyze qualitative data while descriptive statistics were used to analyze the demographic data. Findings indicate that through the Makuleke Contractual Park (MCP) tourism project, the community benefited economically in terms of employment and creation of small & medium tourism related enterprises which provided a livelihood strategy for the community. Through the projects’ partnerships with private investors, there was a ripple effect from the economic capital contribution to other community capitals (physical, human, social and natural). Community-driven tourism projects (CDTP) if well managed can transform community capital resources to economic multipliers which can be a livelihood strategy for the community.
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Johansen, Katja S. "Discovery and industrial applications of lytic polysaccharide mono-oxygenases." Biochemical Society Transactions 44, no. 1 (February 9, 2016): 143–49. http://dx.doi.org/10.1042/bst20150204.
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The recent discovery of copper-dependent lytic polysaccharide mono-oxygenases (LPMOs) has opened up a vast area of research covering several fields of application. The biotech company Novozymes A/S holds patents on the use of these enzymes for the conversion of steam-pre-treated plant residues such as straw to free sugars. These patents predate the correct classification of LPMOs and the striking synergistic effect of fungal LPMOs when combined with canonical cellulases was discovered when fractions of fungal secretomes were evaluated in industrially relevant enzyme performance assays. Today, LPMOs are a central component in the Cellic CTec enzyme products which are used in several large-scale plants for the industrial production of lignocellulosic ethanol. LPMOs are characterized by an N-terminal histidine residue which, together with an internal histidine and a tyrosine residue, co-ordinates a single copper atom in a so-called histidine brace. The mechanism by which oxygen binds to the reduced copper atom has been reported and the general mechanism of copper–oxygen-mediated activation of carbon is being investigated in the light of these discoveries. LPMOs are widespread in both the fungal and the bacterial kingdoms, although the range of action of these enzymes remains to be elucidated. However, based on the high abundance of LPMOs expressed by microbes involved in the decomposition of organic matter, the importance of LPMOs in the natural carbon-cycle is predicted to be significant. In addition, it has been suggested that LPMOs play a role in the pathology of infectious diseases such as cholera and to thus be relevant in the field of medicine.
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Liu, Qiaona, Yun Bai, Sanbao Dong, Jinling Li, Zhifei Song, Shijun Chen, Jie Zhang, and Gang Chen. "Preparation and the Foaming Activity Study of Hydroxymethyl Cetyltrimethyl Ammonium Chloride." Tenside Surfactants Detergents 58, no. 2 (March 1, 2021): 153–60. http://dx.doi.org/10.1515/tsd-2019-2221.
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Abstract In this paper, hydroxymethyl cetyltrimethyl ammonium chloride (HM-CTAC) was prepared from cetyltrimethyl ammonium chloride (CTAC) and formaldehyde with different molar ratios (1:1 to 1: 4). The effects of reaction conditions (molar ratio) on surface properties were studied, including surface tension, foaming ability, high temperature resistance, methanol resistance and salt resistance. The results show that the minimum surface tension of HM-CTAC is lower than that of CTAC, and HM-CTAC (1:1) has the lowest surface tension of 31.89 mN · m–1. The foam volume of HM-CTAC with different molar ratios is higher than that of CTAC, and HM-CTAC (1:4) has a high foam volume of 435 mL. Compared to CTAC, the HM-CTAC under different reaction conditions has higher temperature resistance. At the methanol content of 10 wt.%, the initial foam volume of HM-CTAC is higher than that of CTAC, and the initial foam volume of HM-CTAC (1:2) is the highest with a volume of 21.5 mL. Among all the surfactants prepared under different reaction conditions, HM-CTAC (1:3) has the highest salt resistance with a relatively stable change in foam volume under different salt contents.
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Takagi, M., T. Nakahata, K. Koike, T. Kobayashi, K. Tsuji, S. Kojima, T. Hirano, A. Miyajima, K. Arai, and T. Akabane. "Stimulation of connective tissue-type mast cell proliferation by crosslinking of cell-bound IgE." Journal of Experimental Medicine 170, no. 1 (July 1, 1989): 233–44. http://dx.doi.org/10.1084/jem.170.1.233.
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Crosslinking of cell-bound IgE on mouse connective tissue-type mast cells (CTMC) by multivalent antigen or anti-IgE antibody induced clonal growth of CTMC in methylcellulose culture containing IL-3. Continuous presence of antigen, IgE antibody, and IL-3 in culture was required for extensive proliferation of CTMC. Optimal concentrations of antigen and anti-IgE antibody for proliferation of sensitized CTMC approximately corresponded to those for maximal histamine release from the cells, and it was observed that most dividing cells stimulated by antigen had pericellular degranulation halos in culture. Experiments of both single cell culture and serum free culture provided evidence for a direct effect of antigen stimulation on proliferation of CTMC. Neither accessory cells nor some factors in FCS were required for the clonal growth of CTMC in our culture condition. Compound 48/80, a direct stimulator of CTMC, also triggered histamine release from CTMC but failed to support their proliferation. These results suggest that stimulation of CTMC via IgE receptors not only triggers the release of chemical mediators from the cells but induces clonal growth of CTMC in the presence of IL-3. Our data indicate the possibility that antigen stimulation may play another role in the proliferation of CTMC.
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Tsuji, K., T. Nakahata, M. Takagi, T. Kobayashi, A. Ishiguro, T. Kikuchi, K. Naganuma, K. Koike, A. Miyajima, and K. Arai. "Effects of interleukin-3 and interleukin-4 on the development of "connective tissue-type" mast cells: interleukin-3 supports their survival and interleukin-4 triggers and supports their proliferation synergistically with interleukin-3." Blood 75, no. 2 (January 15, 1990): 421–27. http://dx.doi.org/10.1182/blood.v75.2.421.421.
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Abstract We examined the effects of interleukin-3 (IL-3) and interleukin-4 (IL- 4) on connective tissue-type mast cells (CTMC) purified from murine peritoneal cells. Although both factors failed to induce extensive proliferation of CTMC, they stimulated CTMC proliferation synergistically in a dose-dependent manner. Pretreatment of CTMC with IL-3 and/or IL-4 indicated that the sustained presence of both factors was required for the development of type 1 mast cell colonies. The delayed addition of IL-3 to cultures of purified CTMC with IL-4 induced no colony formation, while the delayed addition of IL-4 to cultures with IL-3, even on day 28 of culture, induced type 1 colony formation. In replating type 1 colonies induced by IL-3 and IL-4 to secondary cultures with IL-3 alone, few secondary colonies developed. However, the delayed addition of IL-4 to the secondary culture induced many type 1 colonies. The purified CTMC cultured with IL-3 retained the morphological and cytochemical characteristics of CTMC, as well as proliferative ability. These observations indicate that IL-3 supports the survival of CTMC in methylcellulose culture and that IL-4 triggers and supports CTMC proliferation synergistically with IL-3. The serum- free culture of purified CTMC and the culture of single CTMC demonstrated that the synergistic effect of IL-3 and IL-4 on colony growth and the surviving effect of IL-3 on CTMC require no influence from accessory cells or other humoral factors.
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Tsuji, K., T. Nakahata, M. Takagi, T. Kobayashi, A. Ishiguro, T. Kikuchi, K. Naganuma, K. Koike, A. Miyajima, and K. Arai. "Effects of interleukin-3 and interleukin-4 on the development of "connective tissue-type" mast cells: interleukin-3 supports their survival and interleukin-4 triggers and supports their proliferation synergistically with interleukin-3." Blood 75, no. 2 (January 15, 1990): 421–27. http://dx.doi.org/10.1182/blood.v75.2.421.bloodjournal752421.
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We examined the effects of interleukin-3 (IL-3) and interleukin-4 (IL- 4) on connective tissue-type mast cells (CTMC) purified from murine peritoneal cells. Although both factors failed to induce extensive proliferation of CTMC, they stimulated CTMC proliferation synergistically in a dose-dependent manner. Pretreatment of CTMC with IL-3 and/or IL-4 indicated that the sustained presence of both factors was required for the development of type 1 mast cell colonies. The delayed addition of IL-3 to cultures of purified CTMC with IL-4 induced no colony formation, while the delayed addition of IL-4 to cultures with IL-3, even on day 28 of culture, induced type 1 colony formation. In replating type 1 colonies induced by IL-3 and IL-4 to secondary cultures with IL-3 alone, few secondary colonies developed. However, the delayed addition of IL-4 to the secondary culture induced many type 1 colonies. The purified CTMC cultured with IL-3 retained the morphological and cytochemical characteristics of CTMC, as well as proliferative ability. These observations indicate that IL-3 supports the survival of CTMC in methylcellulose culture and that IL-4 triggers and supports CTMC proliferation synergistically with IL-3. The serum- free culture of purified CTMC and the culture of single CTMC demonstrated that the synergistic effect of IL-3 and IL-4 on colony growth and the surviving effect of IL-3 on CTMC require no influence from accessory cells or other humoral factors.
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Wu, Wei-Ming, and Yi-Chun Liao. "Downregulation of C-Terminal Tensin-Like Protein (CTEN) Suppresses Prostate Cell Proliferation and Contributes to Acinar Morphogenesis." International Journal of Molecular Sciences 19, no. 10 (October 16, 2018): 3190. http://dx.doi.org/10.3390/ijms19103190.
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C-terminal tensin-like protein (CTEN) is a member of tensin family, which is crucial for the assembly of cell-matrix adhesome. Unlike other tensins, CTEN is selectively expressed only in a few tissues such as the prostate. However, the biological relevance of CTEN in normal prostate is poorly understood. In this study, we revealed that CTEN is selectively expressed in the prostate epithelial cells and enriched in the basal compartment. Knockdown of CTEN in RWPE-1 cells suppresses cell proliferation and results in G1/S cell cycle arrest as well as the accumulation of cyclin-dependent kinase (CDK) inhibitors, p21 and p27. Moreover, the expression of CTEN is decreased during acinar morphogenesis using Matrigel-based three-dimensional (3D) culture. In the course of acinar formation, induction of CTEN reactivates focal adhesion kinase (FAK) Y397 phosphorylation and disrupts the acini structure. This study, to our knowledge, is the first report demonstrating that downregulation of CTEN is required for luminal differentiation and acinar formation.
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Faber, Anne, Christoph Roderburg, Frederik Wein, Rainer Saffrich, Anja Seckinger, Kerstin Horsch, Anke Diehlmann, et al. "The Many Facets of SDF-1α, CXCR4 Agonists and Antagonists on Hematopoietic Progenitor Cells." Journal of Biomedicine and Biotechnology 2007 (2007): 1–10. http://dx.doi.org/10.1155/2007/26065.
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Stromal cell-derived factor-1alpha (SDF-1α) has pleiotropic effects on hematopoietic progenitor cells (HPCs). We have monitored podia formation, migration, proliferation, and cell-cell adhesion of human HPC under the influence of SDF-1α, a peptide agonist of CXCR4 (CTCE-0214), a peptide antagonist (CTCE-9908), and a nonpeptide antagonist (AMD3100). Whereas SDF-1αinduced migration ofCD34+cells in a dose-dependent manner, CTCE-0214, CTCE-9908, and AMD3100 did not induce chemotaxis in this concentration range albeit the peptides CTCE-0214 and CTCE-9908 increased podia formation. Cell-cell adhesion of HPC to human mesenchymal stromal cells was impaired by the addition of SDF-1α, CTCE-0214, and AMD3100. Proliferation was not affected by SDF-1αor its analogs. Surface antigen detection of CXCR4 was reduced upon treatment with SDF-1αor AMD3100 and it was enhanced by CTCE-9908. Despite the fact that all these molecules target the same CXCR4 receptor, CXCR4 agonists and antagonists have selective effects on different functions of the natural molecule.
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Hassan, Bardes B., Lucas A. Altstadt, Wessel P. Dirksen, Said M. Elshafae, and Thomas J. Rosol. "Canine Thyroid Cancer: Molecular Characterization and Cell Line Growth in Nude Mice." Veterinary Pathology 57, no. 2 (February 21, 2020): 227–40. http://dx.doi.org/10.1177/0300985819901120.
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Thyroid cancer is the most common endocrine malignancy in dogs. Dogs and humans are similar in the spontaneous development of thyroid cancer and metastasis to lungs; however, thyroid cancer has a higher incidence of metastasis in dogs. This study developed a preclinical nude mouse model of canine thyroid cancer using a canine thyroid adenocarcinoma cell line (CTAC) and measured the expression of important invasion and metastasis genes in spontaneous canine thyroid carcinomas and CTAC cells. CTAC cells were examined by electron microscopy. Short tandem repeat analysis was performed for both the original neoplasm and CTAC cells. CTAC cells were transduced with luciferase and injected subcutaneously and into the tail vein. Tumors and metastases were monitored using bioluminescent imaging and confirmed with gross necropsy and histopathology. Invasion and metastasis genes were characterized in 8 follicular thyroid carcinomas (FTCs), 4 C-cell thyroid carcinomas, 3 normal thyroids, and CTAC cells. CTAC cells grew well as xenografts in the subcutis, and they resembled the primary neoplasm. Metastasis to the kidney and lung occurred infrequently following subcutaneous and tail vein injection of CTAC cells. STR analysis confirmed that CTAC cells were derived from the original neoplasm and were of canine origin. Finally, 24 genes were differentially expressed in spontaneous canine thyroid carcinomas, CTAC, and normal thyroids. This study demonstrated the usefulness of a nude mouse model of experimental canine thyroid carcinoma and identified potential molecular targets of canine follicular and C-cell thyroid carcinoma.
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Puri, N. K., E. Crivelli, M. Cardamone, R. Fiddes, J. Bertolini, B. Ninham, and M. R. Brandon. "Solubilization of growth hormone and other recombinant proteins from Escherichia coli inclusion bodies by using a cationic surfactant." Biochemical Journal 285, no. 3 (August 1, 1992): 871–79. http://dx.doi.org/10.1042/bj2850871.
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Recombinant pig growth hormone (rPGH) was solubilized from inclusion bodies by using the cationic surfactant cetyltrimethylammonium chloride (CTAC). The solubilizing action of CTAC appeared to be dependent on the presence of a positively charged head group, as a non-charged variant was inactive. Relatively low concentrations of CTAC were required for rapid solubilization, and protein-bound CTAC was easily removed by ion-exchange chromatography. Compared with solubilization and recovery of rPGH from inclusion bodies with 7.5 M-urea and 6 M-guanidinium chloride, the relative efficiency of solubilization was lower with CTAC. However, superior refolding efficiency resulted in final yields of purified rPGH being in the order of CTAC greater than urea greater than or equal to guanidinium chloride. Detailed comparison of the different rPGH preparations as well as pituitary-derived growth hormone by h.p.l.c., native PAGE, c.d. spectral analysis and radioreceptor-binding assay showed that the CTAC-derived rPGH was essentially indistinguishable from the urea and guanidinium chloride preparations. The CTAC-derived rPGH was of greater biopotency than pituitary-derived growth hormone. The advantages of CTAC over urea and guanidinium chloride for increasing recovery of monomeric rPGH by minimizing aggregation during refolding in vitro were also found with recombinant sheep interleukin-I beta and a sheep insulin-like growth factor II fusion protein. In addition, the bioactivity of the CTAC-derived recombinant interleukin-1 beta was approximately ten-fold greater than that of an equivalent amount obtained from urea and guanidinium chloride preparations. It is concluded that CTAC represents, in general, an excellent additional approach or a superior alternative to urea and in particular guanidinium chloride for solubilization and recovery of bioactive recombinant proteins from inclusion bodies.
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Klaassen, Zachary, Abhay A. Singh, Lauren Howard, Martha K. Terris, William J. Aronson, Matthew R. Cooperberg, Christopher L. Amling, Christopher J. Kane, Lionel Lloyds Banez, and Stephen J. Freedland. "Is clinical stage T2C prostate cancer intermediate- or high-risk disease?" Journal of Clinical Oncology 32, no. 4_suppl (February 1, 2014): 110. http://dx.doi.org/10.1200/jco.2014.32.4_suppl.110.
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110 Background: Clinical stage T2c (cT2c) is an indeterminate factor in the algorithm for prostate cancer (CaP) risk stratification. According to the D’Amico risk stratification and the American Urological Association (AUA) guidelines, cT2c is high−risk, whereas the National Comprehensive Cancer Network (NCCN) and EUA classify cT2c as intermediate−risk. Since determining whether cT2c is intermediate- or high-risk has implications for treatment, it is important to define what exact risk cT2c portends. Thus, we sought to assess whether cT2c tumors, without associated other high−risk factors (cT2c not otherwise specified (cT2c−nos)), behave as intermediate− or high−risk by analyzing biochemical recurrence (BCR) after radical prostatectomy (RP). Methods: We retrospectively analyzed 2,759 men who underwent RP from 1988 to 2011 from the Shared Equal Access Regional Cancer Hospital (SEARCH) database. Comparisons in time to BCR between cT2c−nos patients and intermediate−risk (prostate-specific antigen [PSA] 10 to 20 ng/ml or Gleason sum (GS) =7 or cT2b), and high−risk (PSA greater than 20 ng/ml, GS 8 to 10, cT3) patients was performed using log−rank test and Cox proportional hazards analyses. Given changes in CaP, we adjusted for year of surgery (continuous) and to adjust for case mix among centers contributing to SEARCH we included a categorical term for center. Results: A total of 99 men (4%) were classified as cT2c−nos. During a median follow-up of 66 months (IQR: 34−101 months), cT2c−nos patients had similar BCR risk as intermediate-risk (p=0.27), but significantly lower BCR risk versus high-risk patients (p<0.001, Figure). After adjusting for year and center and compared to low-risk disease, the HRs for cT2c−nos patients was similar to those with intermediate-risk (HR 1.90 vs. 2.28). When specifically compared to intermediate-and high-risk patients, and after adjusting for year and center, cT2c−nos patients had outcomes comparable to intermediate−risk (p=0.44), but significantly better than high-risk patients (HR 0.55; 95%CI 0.38,0.78; p=0.001). Conclusions: BCR risk for patients with clinical stage T2c was comparable to men who had intermediate-risk disease and significantly better than men with high-risk CaP. These findings suggest men with cT2c disease should be offered treatment options for intermediate-risk CaP.
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Liao, Yi-Chun, Lizhen Si, Ralph W. deVere White, and Su Hao Lo. "The phosphotyrosine-independent interaction of DLC-1 and the SH2 domain of cten regulates focal adhesion localization and growth suppression activity of DLC-1." Journal of Cell Biology 176, no. 1 (December 26, 2006): 43–49. http://dx.doi.org/10.1083/jcb.200608015.
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The tensin family member cten (C-terminal tensin like) is an Src homology 2 (SH2) and phosphotyrosine binding domain–containing focal adhesion molecule that may function as a tumor suppressor. However, the mechanism has not been well established. We report that cten binds to another tumor suppressor, deleted in liver cancer 1 (DLC-1), and the SH2 domain of cten is responsible for the interaction. Unexpectedly, the interaction between DLC-1 and the cten SH2 domain is independent of tyrosine phosphorylation of DLC-1. By site-directed mutagenesis, we have identified several amino acid residues on cten and DLC-1 that are essential for this interaction. Mutations on DLC-1 perturb the interaction with cten and disrupt the focal adhesion localization of DLC-1. Furthermore, these DLC-1 mutants have lost their tumor suppression activities. When these DLC-1 mutants were fused to a focal adhesion targeting sequence, their tumor suppression activities were significantly restored. These results provide a novel mechanism whereby the SH2 domain of cten-mediated focal adhesion localization of DLC-1 plays an essential role in its tumor suppression activity.
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Shelburne, J., and D. Howell. "Electron Microscopy in Tumor Diagnosis in 2001." Microscopy and Microanalysis 7, S2 (August 2001): 590–91. http://dx.doi.org/10.1017/s1431927600029020.
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Immunohistochemistry (IH) has revolutionized tumor diagnosis in recent years. The antigenic/molecular information now available is extraordinarily helpful not only in clinical diagnostic work, but also in improving our understanding of the cell biology of neoplasia. As a result, there is now less need for diagnostic conventional transmission electron microscopic (CTEM) studies on neoplasms. For example, lymphomas and leukemias are now largely defined by IH and related techniques such as flow cytometry, not CTEM. A postive S-100 stain in the right setting usually obviates the need to search for melanosomes.However, CTEM is still a useful tool for surgical pathologists. One important advantage CTEM has over IH is that - like gross observations and the classic H&E section - CTEM can detect features not suspected in advance. This property of CTEM makes it particularly valuable in studying complex and unusual patients/tumors.
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Durgan, David J., Justin K. Smith, Margaret A. Hotze, Oluwaseun Egbejimi, Karalyn D. Cuthbert, Vlad G. Zaha, Jason R. B. Dyck, E. Dale Abel, and Martin E. Young. "Distinct transcriptional regulation of long-chain acyl-CoA synthetase isoforms and cytosolic thioesterase 1 in the rodent heart by fatty acids and insulin." American Journal of Physiology-Heart and Circulatory Physiology 290, no. 6 (June 2006): H2480—H2497. http://dx.doi.org/10.1152/ajpheart.01344.2005.
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The molecular mechanism(s) responsible for channeling long-chain fatty acids (LCFAs) into oxidative versus nonoxidative pathways is (are) poorly understood in the heart. Intracellular LCFAs are converted to long-chain fatty acyl-CoAs (LCFA-CoAs) by a family of long-chain acyl-CoA synthetases (ACSLs). Cytosolic thioesterase 1 (CTE1) hydrolyzes cytosolic LCFA-CoAs to LCFAs, generating a potential futile cycle at the expense of ATP utilization. We hypothesized that ACSL isoforms and CTE1 are differentially regulated in the heart during physiological and pathophysiological conditions. Using quantitative RT-PCR, we report that the five known acsl isoforms ( acsl1, acsl3, acsl4, acsl5, and acsl6) and cte1 are expressed in whole rat and mouse hearts, as well as adult rat cardiomyocytes (ARCs). Streptozotocin-induced insulin-dependent diabetes (4 wk) and fasting (≤24 h) both dramatically induced cte1 and repressed acsl6 mRNA, with no significant effects on the other acsl isoforms. In contrast, high-fat feeding (4 wk) induced cte1 without affecting expression of the acsl isoforms in the heart. Investigation into the mechanism(s) responsible for these transcriptional changes uncovered roles for peroxisome proliferator-activated receptor-α (PPARα) and insulin as regulators of specific acsl isoforms and cte1 in the heart. Culturing ARCs with oleate (0.1–0.4 mM) or the PPARα agonists WY-14643 (1 μM) and fenofibrate (10 μM) consistently induced acsl1 and cte1. Conversely, PPARα null mouse hearts exhibited decreased acsl1 and cte1 expression. Culturing ARCs with insulin (10 nM) induced acsl6, whereas specific loss of insulin signaling within the heart (cardiac-specific insulin receptor knockout mice) caused decreased acsl6 expression. Our data expose differential regulation of acsl isoforms and cte1 in the heart, where acsl1 and cte1 are PPARα-regulated genes, whereas acsl6 is an insulin-regulated gene.
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Oktavia, Eva. "A Comparative Study between Thoracic Epidural Anesthesia and General Anesthesia for Patients Who Underwent Modified Radical Mastectomy with Axillary Lymph Node Dissection in De La Salle University Medical Center." Indonesian Biomedical Journal 7, no. 2 (August 1, 2015): 111. http://dx.doi.org/10.18585/inabj.v7i2.77.
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BACKGROUND: To compare the recovery time and other related clinical outcomes among patients who underwent Modified Radical Mastectomy (MRM) with axillary lymph node dissection under Continuous Thoracic Epidural Anesthesia (CTEA) and General Endotracheal Tube Anesthesia (GETA).METHODS: A retrospective cross-sectional study with 70 patients who underwent MRM in De La Salle University Medical Centre (DLSUMC), categorized into GETA and CTEA group consisted of 35 patients each. Per oral premedications 15 mg midazolam, 40 mg omeprazole and 10 mg metoclopramide were given 1 hour prior to surgery. Intra-operative hypotension/hypertension, tachycardia/bradycardia status, length of Post-Anesthesia Care Unit (PACU) and hospital stay, and Post Operative Nausa and Vomiting (PONV) incidence were compared between 2 groups.RESULTS: Preoperatively, there were no significant differences between the groups in terms of subject characteristic. Intra-operatively, hypertension was more frequent in GETA group (28.6% vs. 0%), while hypotension was more frequent in the CTEA (80% vs. 57.1%). Tachycardia was more frequent in GETA group (46.6% vs. 0%), meanwhile bradycardia was more frequent in CTEA (40% vs. 17.1%). Postoperatively, the GETA group had shorter PACU stay than CTEA (230 mins vs. 267 mins), but CTEA group had a shorter time of hospital stay compared to GETA (58.1 hours vs. 67.7 hours). The incidence of PONV were comparable among the two groups (GETA 46.7% vs. CTEA 50%). Statistically there were no significant differences between the two groups in all of the above characteristics.CONCLUSION: CTEA technique has no effect on inducing hypertension and tachycardia, but hypotension and bradycardia may occur. Although GETA gives shorter PACU duration, CTEA gives shorter hospital stay. This gave impression that CTEA is an effective alternative technique to GETA in patients who underwent MRM with axillary dissection.KEYWORDS: modified radical mastectomy, general anesthesia, epidural anesthesia
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Lange, Marcos Christiano, Viviane Flumignan Zétola, Admar Moraes de Souza, Élcio Juliato Piovesan, Juliano André Muzzio, Francisco Manoel Branco Germiniani, and Lineu César Werneck. "Transcranial Doppler for patent foramen ovale screening: is there a good correlation with transesophageal echocardiography?" Arquivos de Neuro-Psiquiatria 66, no. 4 (December 2008): 785–89. http://dx.doi.org/10.1590/s0004-282x2008000600001.
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Right-to-left shunt (RLS) can be identified by contrast-enhanced transcranial Doppler (cTCD) in patent foramen ovale (PFO) patients. AIM: To evaluate cTCD for PFO screening comparing it to cTEE. METHOD: 45 previous cTCD performed for PFO diagnosis and correlated its findings with cTEE. Patients were submitted to a cTCD standardized technique and were divided in two groups according to RLS: Group 1, patients with a positive RLS and Group 2 when RLS was negative. RESULTS: 29 (65%) patients were included in group 1 and 16 (35%) in group 2. PFO confirmation by cTEE was performed in 28 (62%) patients. cTCD had a 92.85% sensitivity, 82.35% specificity, 89.65% positive predictive value and 87.5% negative predictive value when compared to cTEE for PFO diagnosis. CONCLUSION: Standardized technique cTCD allows for RLS visualization in PFO patients with a good correlation with cTEE and can be used as a screening test before cTEE.
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Yanti, T. Setiawan, and B. W. Lay. "Clitoria ternatea ethanolic extract prevents dental caries via inhibiting Streptococcus mutans growth and quorum sensing." Food Research 5, no. 2 (April 30, 2021): 492–97. http://dx.doi.org/10.26656/fr.2017.5(2).508.
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Our previous study demonstrated that the blue butterfly pea flower (Clitoria ternatea) had several pharmacological effects to treat inflammatory-related diseases, including edema and diabetes. However, its benefit for preventing dental caries and protecting the tooth has not been explored yet. Here, we investigated whether C. ternatea ethanolic extract (CTEE) prevented dental caries through antibacterial and antiquorum sensing activities toward oral pathogen Streptococcus mutans in vitro. CTEE was made by using kinetic maceration in ethanol. Antibacterial activity of CTEE against S. mutans was tested using disk diffusion agar and microdilution assays. Quorum sensing system employed Chromobacterium violaceum bacteria to produce violacein, and CTEE at various concentrations was tested for its antiquorum sensing activity to inhibit the violacein production. Our results demonstrated that CTEE at 1 mg/mL showed a significant inhibition >90% against S. mutans, indicating its MIC value. For the quorum sensing system, CTEE at the lowest concentration (0.25 mg/mL) significantly inhibit up to 68% of violacein produced by C. violaceum. These data indicate that CTEE may act as a natural oral functional food with antibacterial and antiquorum sensing activities for the prevention of dental caries.
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Fleming, Jason C., Jeongmin Woo, Karwan Moutasim, Christopher J. Hanley, Steven J. Frampton, Oliver Wood, Matthew Ward, et al. "CTEN Induces Tumour Cell Invasion and Survival and Is Prognostic in Radiotherapy-Treated Head and Neck Cancer." Cancers 12, no. 10 (October 13, 2020): 2963. http://dx.doi.org/10.3390/cancers12102963.
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Head and neck squamous cell carcinoma (HNSCC) is a heterogenous disease treated with surgery and/or (chemo) radiotherapy, but up to 50% of patients with late-stage disease develop locoregional recurrence. Determining the mechanisms underpinning treatment resistance could identify new therapeutic targets and aid treatment selection. C-terminal tensin-like (CTEN) is a member of the tensin family, upregulated in several cancers, although its expression and function in HNSCC are unknown. We found that CTEN is commonly upregulated in HNSCC, particularly HPV−ve tumours. In vitro CTEN was upregulated in HPV−ve (n = 5) and HPV+ve (n = 2) HNSCC cell lines. Stable shRNA knockdown of CTEN in vivo significantly reduced tumour growth (SCC-25), and functional analyses in vitro showed that CTEN promoted tumour cell invasion, colony formation and growth in 3D-culture (SCC-25, Detroit 562). RNA sequencing of SCC-25 cells following CTEN siRNA knockdown identified 349 differentially expressed genes (logFC > 1, p < 0.05). Gene ontology analysis highlighted terms relating to cell locomotion and apoptosis, consistent with in vitro findings. A membrane-based antibody array confirmed that CTEN regulated multiple apoptosis-associated proteins, including HSP60 and cleaved caspase-3. Notably, in a mixed cohort of HPV+ve and HPV−ve HNSCC patients (n = 259), we found a significant, independent negative association of CTEN with prognosis, limited to those patients treated with (chemo)radiotherapy, not surgery, irrespective of human papillomavirus (HPV) status. These data show that CTEN is commonly upregulated in HNSCC and exerts several functional effects. Its potential role in modulating apoptotic response to therapy suggests utility as a predictive biomarker or radio-sensitising target.
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Singh, Abhay A., Leah Gerber, Stephen J. Freedland, William J. Aronson, Martha K. Terris, Joseph C. Presti, Christopher L. Amling, and Lionel Lloyds Banez. "Is clinical stage T2c prostate cancer intermediate- or high-risk disease? Results from the SEARCH database." Journal of Clinical Oncology 30, no. 5_suppl (February 10, 2012): 123. http://dx.doi.org/10.1200/jco.2012.30.5_suppl.123.
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123 Background: Clinical stage T2c is a nebulous factor in the algorithm for prostate cancer risk stratification. According to D’Amico risk stratification cT2c is high-risk category where NCCN guidelines place this stage in intermediate-risk. As diagnostic work up with the use of MRI continues to escalate clinical staging may become more important. As cT2c represents a possible decision fork in treatment decisions we sought to investigate which risk group the clinical behavior of cT2c tumors more closely resembles. Methods: We retrospectively analyzed data from 1089 men who underwent radical prostatectomy (RP) from 1988 to 2009 who did not have low-risk CaP from the SEARCH database. We compared time to BCR between men with cT2c disease, those with intermediate-risk (PSA 10-20 ng/ml or Gleason sum (GS) =7), and those with high-risk (PSA>20 ng/ml, GS 8-10, cT3) using Cox regression models adjusting for age, race, year of RP, center, and percent cores positive. We also compared predictive accuracy of two Cox models wherein cT2c was considered either intermediate- or high-risk by calculating concordance index c. Results: A total of 68 men (3.4%) had cT2c tumors. After a median follow-up of 47.5 months, there was no difference in BCR risk between men with intermediate-risk CaP and those with cT2c tumors (HR=0.90; p=0.60). In contrast, there was a trend for men with high-risk CaP to have nearly 50% increased BCR risk compared to men with cT2c tumors (HR=1.50; 95% CI=0.97-2.30; p=0.07) which did not reach statistical significance. Concordance index c was higher in the Cox model wherein cT2c tumors were considered intermediate-risk (c=0.6147) as opposed to high-risk (c=0.6106). Conclusions: BCR risk for patients with clinical stage T2c was more comparable to men who had intermediate-risk CaP than men with high-risk. In addition, a model which incorporates cT2c disease as intermediate-risk has better predictive accuracy. These findings suggest men with cT2c disease should be offered treatment options for men with intermediate-risk CaP. As clinical staging more routinely incorporates MRI there is the potential to better identify bilateral organ-confined CaP and further establish risk classification.
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Irfannuddin, Irfannuddin, Nur Rachmat Lubis, and Febian Aji Wicaksono. "Evaluation of CTEV Management in Children's Walking Ability Assessed by "BANGLA" Club Foot Tool Score System." Bioscientia Medicina : Journal of Biomedicine and Translational Research 4, no. 1 (January 1, 2020): 48–57. http://dx.doi.org/10.32539/bsm.v4i1.113.
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Abstract
Congenital talipes equinovarus (CTEV) or clubfoot is a congenital deformity that involves an abnormal position of the calcaneonaviculare complex. "Bangla clubfoot tool score system" is an assessment that indicates the effectiveness of CTEV management. The purpose of this study was to evaluate the management of CTEV on children's ability to walk as assessed by the Bangla clubfoot tool score system.
A cross sectional study was conducted at the Hospital Dr. Mohammad Hoesin Palembang. There were 24 CTEV patients who received CTEV management before the age of 3 years and were not associated with a neurological disorder. A comparison of the average total Bangla clubfoot score tool system was analyzed by assessing parental satisfaction, walking ability and clinical examination.
The majority of CTEV patients were women (58.3%) with and mostly being treated before 1 year old (79.2%). The most types of CTEV were bilateral (70.8%), and most of them performed surgery (66.7%). Assessment with the Bangla clubfoot tool system shows that the level of parental satisfaction is sufficient, gait is good, but physical foot examination is poor (20%). The score is influenced by age at first therapy and compliance using the brace.
Parents must continue to support their children to undergo integrated management after therapy to maintain their walking ability.
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Urbaniak-Kujda, Donata M., Katarzyna L. Kapelko-Slowik, Bozena Jazwiec, Joanna Maj, Jaroslaw Dybko, Miroslaw Slowik, Dariusz Wolowiec, Irena Frydecka, and Kazimierz Kuliczkowski. "CD8+CD28− in Peripheral Blood of Patients with Cutaneus T-Cell Lymphoma (CTLC) in Different Clinical Stages." Blood 110, no. 11 (November 16, 2007): 4395. http://dx.doi.org/10.1182/blood.v110.11.4395.4395.
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Abstract Background: Patients with CTLC usually have a poor immune response. CD8+CD28− T cells are new types of immune suppressor cells. The study was to analyze the level and changes of them in peripheral blood with CTLC, their effects on immunosupression of CTLC, the influence factors to provide reference for treatment of CTLC patients. Patients and Methods: 27 patients with CTLC were enrolled in the study: 8 women and 19 men aged 35–81, medium 57. According to Ann Arbor classification stage I was represented by 1 patient, stage II -6 patients, stage III - 5 patients, stage IV - 6 patients and stage early stage (premycoticus) was represented by 8 patients. Peripheral blood samples were assessed from all patients with CTLC before treatment and 8 normals. Percentage CD8+CD28− T cells was analyzed by flow cytometry. Result: Compared with control group percentage of CD8+CD28− T cells was significantly higher in patients group p=0,005 (test U Mann Whitney). There were no significant differences of T-cells in among patients with different clinical stages. We revealed the correlation between CD8+CD28− cells and advanced clinical stage r=0,54, p=0,035 (Spearman correlation test). Conclusion: Percentage of CD8+CD28− T cells is increased in peripheral blood in CTLC patients, and correlates with advanced stage.
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Haig, DM, JF Huntley, A. MacKellar, GF Newlands, L. Inglis, R. Sangha, D. Cohen, A. Hapel, SJ Galli, and HR Miller. "Effects of stem cell factor (kit-ligand) and interleukin-3 on the growth and serine proteinase expression of rat bone-marrow-derived or serosal mast cells." Blood 83, no. 1 (January 1, 1994): 72–83. http://dx.doi.org/10.1182/blood.v83.1.72.bloodjournal83172.
Full textAbstract:
The effects of rat stem-cell factor (SCF) and interleukin-3 (IL-3), alone or in combination, on the in vitro growth and serine proteinase expression of rat serosal/connective-tissue mast cells (CTMC) or bone marrow-derived mast cells (BMMC) were examined. Rat SCF stimulated the growth of both CTMC and BMMC. IL-3 stimulated BMMC growth to a lesser extent than did SCF, whereas CTMC numbers did not increase in IL-3. However, SCF and IL-3 had synergistic effects on the growth of both BMMC and CTMC. SCF favoured the maintenance of rat mast cell proteinase- I (RMCP-I) in CTMC, but did not induce detectable production of RMCP-I in BMMC. In contrast, when IL-3 or lymph node-conditioned medium (LNCM) was added to SCF, a subpopulation of CTMC expressed and stored the soluble proteinase RMCP-II. In BMMC, the RMCP-II content of cells maintained in SCF was significantly less than that of cells maintained in IL-3 or LNCM. RMCP-II also appeared in the supernatants of BMMC, especially when BMMC numbers were increasing rapidly in SCF with or without IL-3 or LNCM. Thus, SCF and IL-3 can regulate the growth of rat BMMC and CTMC, as well as influence their production and release of proteinases.
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Xing, Jun, Jayant J. Jayasundar, Yexin Ouyang, and Wen-Ji Dong. "Förster Resonance Energy Transfer Structural Kinetic Studies of Cardiac Thin Filament Deactivation." Journal of Biological Chemistry 284, no. 24 (April 15, 2009): 16432–41. http://dx.doi.org/10.1074/jbc.m808075200.
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Cardiac thin filament deactivation is initiated by Ca2+ dissociation from troponin C (cTnC), followed by multiple structural changes of thin filament proteins. These structural transitions are the molecular basis underlying the thin filament regulation of cardiac relaxation, but the detailed mechanism remains elusive. In this study Förster resonance energy transfer (FRET) was used to investigate the dynamics and kinetics of the Ca2+-induced conformational changes of the cardiac thin filaments, specifically the closing of the cTnC N-domain, the cTnC-cTnI (troponin I) interaction, and the cTnI-actin interaction. The cTnC N-domain conformational change was examined by monitoring FRET between a donor (AEDANS) attached to one cysteine residue and an acceptor (DDPM) attached the other cysteine of the mutant cTnC(L13C/N51C). The cTnC-cTnI interaction was investigated by monitoring the distance changes from residue 89 of cTnC to residues 151 and 167 of cTnI, respectively. The cTnI-actin interaction was investigated by monitoring the distance changes from residues 151 and 167 of cTnI to residue 374 of actin. FRET Ca2+ titrations and stopped-flow kinetic measurements show that different thin filament structural transitions have different Ca2+ sensitivities and Ca2+ dissociation-induced kinetics. The observed structural transitions involving the regulatory region and the mobile domain of cTnI occurred at fast kinetic rates, whereas the kinetics of the structural transitions involving the cTnI inhibitory region was slow. Our results suggest that the thin filament deactivation upon Ca2+ dissociation is a two-step process. One step involves rapid binding of the mobile domain of cTnI to actin, which is kinetically coupled with the conformational change of the N-domain of cTnC and the dissociation of the regulatory region of cTnI from cTnC. The other step involves switching the inhibitory region of cTnI from interacting with cTnC to interacting with actin. The latter processes may play a key role in regulating cross-bridge kinetics.
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Har Li, Karen Kwai, Carmen K. Y. Chuen, Shuk Man Lee, Donald Wong, Ahmed Merzouk, Hassan Salari, Patrick M. P. Yuen, et al. "Short Pulse of a Small Peptide Agonist of Stromal Cell-Derived Factor - 1 (SDF-1) Enhances the Engraftment of Expanded Human Cord Blood Hematopoietic Progenitor Cells in NOD/SCID Mice." Blood 104, no. 11 (November 16, 2004): 404. http://dx.doi.org/10.1182/blood.v104.11.404.404.
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Abstract SDF-1 is the ligand to the chemokine receptor CXCR-4. A small synthetic peptide agonist of SDF-1 (CTCE-0214) has been shown to expand human cord blood hematopoietic stem and progenitor cells. In this study, we investigated whether a brief exposure of expanded cord blood hematopoietic cells to CTCE-0214 can improve engraftment of the cells into NOD/SCID mice. Published in vivo studies demonstrated that the administration of CTCE-0214 to transplanted NOD/SCID mice mobilized human colony forming cells (CFC) and enhanced human thrombopoiesis (Exp Hematol 32, 300, 2004). Our earlier study showed that CTCE-0214 added to single factors of thrombopoietin (TPO), stem cell factor (SCF), or Flt-3 ligand (F3L) synergistically increased the survival of enriched cord blood CD34+ cells (Blood 102, 960a, 2003). In this study, we further investigated the effects of CTCE-0214 on the ex vivo expansion of CD34+ cells to multi-lineage progenitors and the homing and engraftment capacity of expanded human progenitor cells after a short in vitro exposure to the peptide prior to infusion into NOD/SCID mice. Enriched CD34+ cells (MACS) derived from cord blood were cultured for 8 days in serum-free medium QBSF-60 containing TPO (50 ng/ml), SCF (50 ng/nl) and F3L (80 ng/ml) (TSF), with or without CTCE-0214 (0.01 ng/ml) (TSF+CTCE-0214) added at day 4. Progenitor cells expanded for 8 days in the absence of CTCE-0214 were pulsed with the peptide (100 ng/ml) for 4 hours (TSFpCTCE-0214). Results are summarized in Table. CTCE-0214 significantly (N=30, p≤0.05, paired t-test) increased the fold expansion of total nucleated cells (TNC), CD34+ cells, CD34+CD38- cells, CFU-GM, CFU-E, and CFU-MK (total CFC). Expanded progenitor cells (with and without CTCE-0214) were then infused into irradiated NOD/SCID mice. After 6 weeks, enhanced engraftments of human CD45+ cells (p≤0.05, N=21) were demonstrated in the bone marrow (BM) of mice that received cells cultured in TSF+CTCE-0214. Interestingly, a short pulse of cells expanded in TSF to CTCE-0214 for 4 hours also significantly increased the NOD/SCID engraftment (N=18), although no major changes to the in vitro read-out parameters were observed. The mechanism could be associated with the increased homing capacity of progenitor cells after pulsing with CTCE-0214. In conclusion, our results showed that CTCE-0214 enhances the proliferation of early progenitor cells in culture and exposure to the peptide can enhance the engraftment potential of expanded cells in NOD/SCID mice. The SDF-1 peptide agonist could be developed for application to hematopoietic stem cell transplantation and ex vivo expansion. NOD/SCID Engraftment of Expanded Cord Blood Stem Cells TSF TSF+CTCE-0214 TSFpCTCE-0214 *Fold expansion (mean±SE); **% human CD45+ cells in BM of mice TNC* 84.6±10.4 123.5±15.3 88.5±11.2 CD34+* 8.5±1.3 14.1±2.1 9.6±1.6 CD34+CD38−* 24.6±4.8 48.7±8.6 27.5±5.3 Total CFC* 46.9±6.5 87.9±10.7 50.6±6.4 NOD/SCID** 2.8±0.9 6.7±2.5 8.3±4.0
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Zhou, Yue Xi, Xi Miao Zhang, and Qian Zhang. "Researching Knowledge Management and Cultural Innovation of Chinese Traditional Color-Culture for the Purpose of Computer Design." Applied Mechanics and Materials 201-202 (October 2012): 852–55. http://dx.doi.org/10.4028/www.scientific.net/amm.201-202.852.
Full textAbstract:
Chinese traditional color-culture (CTCC) is the important foundation to build the human color cultural discipline. In order to carry on the knowledge management and cultural innovation, the introduction of management principles to CTCC is imperative. Firstly, based on computer technology and interdisciplinary knowledge, the paper uses game theory to analyze the necessity of carrying on the knowledge management and cultural innovation for CTCC. Secondly, the management principles are used to form the sub-systems of CTCC, and to carry on its knowledge management. Finally, we explain the culture innovative ways of the seven sub-systems of CTCC. By carrying on knowledge management and cultural innovation, it can be used in the design-education and the computer design, and in establishing a good foundation for the color-cultural discipline.
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Tubon, Irvin, Chiara Bernardini, Fabiana Antognoni, Roberto Mandrioli, Giulia Potente, Martina Bertocchi, Gabriela Vaca, Augusta Zannoni, Roberta Salaroli, and Monica Forni. "Clinopodium tomentosum (Kunth) Govaerts Leaf Extract Influences in vitro Cell Proliferation and Angiogenesis on Primary Cultures of Porcine Aortic Endothelial Cells." Oxidative Medicine and Cellular Longevity 2020 (August 18, 2020): 1–11. http://dx.doi.org/10.1155/2020/2984613.
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Clinopodium tomentosum (Kunth) Govaerts is an endemic species in Ecuador, where it is used as an anti-inflammatory plant to treat respiratory and digestive affections. In this work, effects of a Clinopodium tomentosum ethanolic extract (CTEE), prepared from aerial parts of the plant, were investigated on vascular endothelium functions. In particularly, angiogenesis activity was evaluated, using primary cultures of porcine aortic endothelial cells (pAECs). Cells were cultured for 24 h in the presence of CTEE different concentrations (10, 25, 50, and 100 μg/ml); no viability alterations were found in the 10-50 μg/ml range, while a slight, but significant, proliferative effect was observed at the highest dose. In addition, treatment with CTEE was able to rescue LPS-induced injury in terms of cell viability. The CTEE ability to affect angiogenesis was evaluated by scratch test analysis and by an in vitro capillary-like network assay. Treatment with 25-50 μg/ml of extract caused a significant increase in pAEC’s migration and tube formation capabilities compared to untreated cells, as results from the increased master junctions’ number. On the other hand, CTEE at 100 μg/ml did not induce the same effects. Quantitative PCR data demonstrated that FLK-1 mRNA expression significantly increased at a CTEE dose of 25 μg/ml. The CTEE phytochemical composition was assessed through HPLC-DAD; rosmarinic acid among phenolic acids and hesperidin among flavonoids were found as major phenolic components. Total phenolic content and total flavonoid content assays showed that flavonoids are the most abundant class of polyphenols. The CTEE antioxidant activity was also showed by means of the DPPH and ORAC assays. Results indicate that CTEE possesses an angiogenic capacity in a dose-dependent manner; this represents an initial step in elucidating the mechanism of the therapeutic use of the plant.
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Tran Trung, Dung, Phuong Nguyen Huy, Tung Pham Son, Thien Chu Dinh, and Toi Chu Dinh. "Anatomical Study of Femoral Condylar Index in Magnetic Resonance Imaging: Implication to Total Knee Replacement Surgery for Vietnamese People." Open Access Macedonian Journal of Medical Sciences 7, no. 24 (December 20, 2019): 4362–67. http://dx.doi.org/10.3889/oamjms.2019.836.
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BACKGROUND: The femoral rotation angle is important element in total knee replacement (TKR).
AIM: To measure this angle, we determine through the axes: the transepicondylar axis (cTEA and sTEA), the posterior condylar axis (PCA), the anteroposterior axis (APA – Whiteside axis).
METHODS: Measuring the angles created by the four axes: cTEA, sTEA, PCA and APA in magnetic resonance imaging (MRI); determining the femoral rotation angle and application TKR.
RESULTS: the angle between APA and cTEA: 90.41° ± 3.35°, the angle between APA and sTEA: 94.47° ± 3.31°, the angle between APA and PCA: 96.40° ± 4.59°, the angle between cTEA and sTEA: 4.00° ± 1.02°, the angle between cTEA and PCA: 6.53° ± 2.55°, the angle between sTEA and PCA: 3.48° ± 1.91°.
CONCLUSION: The angle between sTEA and PCA is the angle that best represents the femoral rotation angle. However, in case of sTEA or PCA is difficult to identify, it can be measure via the APA or cTEA. These angles don’t differ by age, gender and place of knee joint.
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Korte, F. Steven, Erik R. Feest, Maria V. Razumova, An-Yue Tu, and Michael Regnier. "Enhanced Ca2+ binding of cardiac troponin reduces sarcomere length dependence of contractile activation independently of strong crossbridges." American Journal of Physiology-Heart and Circulatory Physiology 303, no. 7 (October 1, 2012): H863—H870. http://dx.doi.org/10.1152/ajpheart.00395.2012.
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Calcium sensitivity of the force-pCa relationship depends strongly on sarcomere length (SL) in cardiac muscle and is considered to be the cellular basis of the Frank-Starling law of the heart. SL dependence may involve changes in myofilament lattice spacing and/or myosin crossbridge orientation to increase probability of binding to actin at longer SLs. We used the L48Q cardiac troponin C (cTnC) variant, which has enhanced Ca2+ binding affinity, to test the hypotheses that the intrinsic properties of cTnC are important in determining 1) thin filament binding site availability and responsiveness to crossbridge activation and 2) SL dependence of force in cardiac muscle. Trabeculae containing L48Q cTnC-cTn lost SL dependence of the Ca2+ sensitivity of force. This occurred despite maintaining the typical SL-dependent changes in maximal force (Fmax). Osmotic compression of preparations at SL 2.0 μm with 3% dextran increased Fmax but not pCa50 in L48Q cTnC-cTn exchanged trabeculae, whereas wild-type (WT)-cTnC-cTn exchanged trabeculae exhibited increases in both Fmax and pCa50. Furthermore, crossbridge inhibition with 2,3-butanedione monoxime at SL 2.3 μm decreased Fmax and pCa50 in WT cTnC-cTn trabeculae to levels measured at SL 2.0 μm, whereas only Fmax was decreased with L48Q cTnC-cTn. Overall, these results suggest that L48Q cTnC confers reduced crossbridge dependence of thin filament activation in cardiac muscle and that changes in the Ca2+ sensitivity of force in response to changes in SL are at least partially dependent on properties of thin filament troponin.
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Zhang, Xi Miao, Yue Xi Zhou, and Hong Yan Yang. "Educational Reform of Importing Chinese Traditional Color Culture into the Design Education after Cultural Innovation." Applied Mechanics and Materials 201-202 (October 2012): 1017–20. http://dx.doi.org/10.4028/www.scientific.net/amm.201-202.1017.
Full textAbstract:
Chinese traditional color culture (CTCC) is an essential foundation of building the human color cultural discipline, also is an important direction of educational reform for perfectly training design talents. Based on interdisciplinary methods, firstly, the paper uses Game theory to analyze the necessity of the knowledge management and cultural innovation for CTCC. Secondly, the paper utilizes the management principles to carry on knowledge management of CTCC, and explains culture innovative ways for it. Finally, we discuss methods of application CTCC and color education reform. Through cultural innovation research of CTCC and the introduction of it into universities design education, it can not only surmount the malpractice of that just has a single modern chromatics without any element of national color culture, but also greatly improve the situation of that the designer has not any principles to abide by.
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Haig, DM, JF Huntley, A. MacKellar, GF Newlands, L. Inglis, R. Sangha, D. Cohen, A. Hapel, SJ Galli, and HR Miller. "Effects of stem cell factor (kit-ligand) and interleukin-3 on the growth and serine proteinase expression of rat bone-marrow-derived or serosal mast cells." Blood 83, no. 1 (January 1, 1994): 72–83. http://dx.doi.org/10.1182/blood.v83.1.72.72.
Full textAbstract:
Abstract The effects of rat stem-cell factor (SCF) and interleukin-3 (IL-3), alone or in combination, on the in vitro growth and serine proteinase expression of rat serosal/connective-tissue mast cells (CTMC) or bone marrow-derived mast cells (BMMC) were examined. Rat SCF stimulated the growth of both CTMC and BMMC. IL-3 stimulated BMMC growth to a lesser extent than did SCF, whereas CTMC numbers did not increase in IL-3. However, SCF and IL-3 had synergistic effects on the growth of both BMMC and CTMC. SCF favoured the maintenance of rat mast cell proteinase- I (RMCP-I) in CTMC, but did not induce detectable production of RMCP-I in BMMC. In contrast, when IL-3 or lymph node-conditioned medium (LNCM) was added to SCF, a subpopulation of CTMC expressed and stored the soluble proteinase RMCP-II. In BMMC, the RMCP-II content of cells maintained in SCF was significantly less than that of cells maintained in IL-3 or LNCM. RMCP-II also appeared in the supernatants of BMMC, especially when BMMC numbers were increasing rapidly in SCF with or without IL-3 or LNCM. Thus, SCF and IL-3 can regulate the growth of rat BMMC and CTMC, as well as influence their production and release of proteinases.
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Shen, Jinwei, Yi Liu, Robert P. Thornburgh, Andrew R. Kreshock, and Matthew L. Wilbur. "Design and optimisation of an aerofoil with active continuous trailing-edge flap." Aeronautical Journal 120, no. 1231 (June 13, 2016): 1468–86. http://dx.doi.org/10.1017/aer.2016.62.
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ABSTRACTThis paper presents the design and optimisation of an aerofoil with active continuous trailing-edge flap (CTEF) investigated as a potential rotorcraft active control device. Several structural cross-section models are developed: high-fidelity NASA STRucture ANalysis (NASTRAN) and University of Michigan/Variational Asymptotic Beam Section Code (UM/VABS) models and a reduced-order analysis model. The validation of the reduced-order model is established by comparing its predictions of CTEF deformations with those of NASTRAN and UM/VABS analyses, which both show good agreement. The 2D aerodynamic characteristics of the CTEF aerofoil are evaluated using XFOIL and Computational Fluid Dynamics (CFD) analyses: FUN3D and Overset Transonic Unsteady Rotor Navier-Stokes (OVERTURNS). XFOIL, coupled with the reduced-order structure model, is adopted for optimisation study. The accuracy of XFOIL in predicting the aerodynamic pressure of the CTEF aerofoil is verified using CFD simulations, which shows sufficient fidelity. The predicted variations of aerodynamic coefficients with a CTEF angle are compared among the aerodynamic analyses. The optimisation process is developed and applied to two bimorph bender configurations: a Macro-Fibre Composite (MFC) solid bender and an MFC stack bender. The solid bender is used to confirm the functioning of the optimisation procedure and to use its optimal layout as a reference to the stack design, the primary design object. A linear tapered shape is found to be the optimum for a MFC solid bender, which generates an average of 63% more CTEF angles than those of an optimal rectangular bender. An optimised MFC stack bender is shown to resemble the shape of the solid bender. A four-ply bimorph is considered the best choice among the stack layouts because of its large output of CTEF angles and relatively less plies required. The CTEF angle produced by the four-ply optimal layout ranges from 7.6° to 5.3° with speeds from 0 to 200m/s at an angle of attack (AoA) of 6°. The reduction in the CTEF angle with AoA is less steep than that with speed, ranging from 6.5° to 5.8° with AoA from 0 to 8° at speed of 166m/s. An average of 14% increase in CTEF angles is achieved through optimisation for the four-ply bimorph.
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Lu, Hongbo, Zhihong Zeng, Yuexi Shi, Sergej Konoplev, Donald Wong, Marina Konopleva, and Michael Andreeff. "Disruption of Leukemia/Stroma Cell Interactions by CXCR4 Antagonist CTCE-9908 Enhances Chemotherapy-Induced Apoptosis in AML." Blood 112, no. 11 (November 16, 2008): 2415. http://dx.doi.org/10.1182/blood.v112.11.2415.2415.
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Abstract The chemokine receptor CXCR4 is critically involved in the migration of hematopoietic cells towards the stromal derived factor (SDF-1α)-producing bone marrow microenvironment. We and others have previously demonstrated that stroma/leukemia interactions mediate protection of leukemic cells from chemotherapy-induced apoptosis (Konopleva, Leukemia 2002). Using a peptide analog of SDF-1α designated CTCE-9908, we tested the hypothesis that CXCR4 inhibition interferes with stromal/leukemia cell interactions resulting in increased sensitivity to chemotherapy. Our results showed that CTCE-9908 significantly inhibits SDF-1α-induced migration of U937 (43% inhibition) and OCI-AML3 cells (40% inhibition) in a dose-dependent manner. In three of the four primary AML samples which expressed CXCR4 on cell surface and migrated in response to SDF-1α, 50 μg/ml CTCE-9908 reduced SDF-1α-induced migration of leukemic blasts (60%, 19% and 50% inhibition respectively). In in vitro co-culture systems, stromal cells significantly protected OCI-AML3 cells from chemotherapy induced apoptosis [no MS-5, 75.2±5.2% annexinV(+); with MS-5, 59±1.1% annexinV(+)]. Western blot analysis revealed that CTCE-9908 inhibits Akt and Erk phosphorylation in a dose-dependent manner in the OCI-AML3 cell line stimulated by SDF-1α. Blockade of CXCR4 expression with CTCE-9908 markedly abrogated the protective effects of stromal cells on OCI-AML3 [Ara-C, 59±1.1% annexinV(+); Ara-C + CTCE-9908, 76.9±1.35 annexinV(+)]. Most importantly, it decreased stroma-mediated protection from AraC-induced apoptosis in four out of five primary AML samples with surface expression of functional CXCR4 (mean increase, 25.1±9.3% compared to chemotherapy alone). In vivo, subcutaneous administration of 1.25mg CTCE-9908 induced mobilization of leukemic cells from primary AML patient transplanted into NOD/Scid-IL2Rγ-KO mice (from 15% to 27% circulating leukemic cells 1 hour post CTCE-9908 injection). Taken together, our data suggest that SDF-1α/CXCR4 interactions contribute to the resistance of leukemic cells to chemotherapy-induced apoptosis via retention of leukemic cells in the bone marrow microenvironment niches. Disruption of these interactions by the potent CXCR4 inhibitor CTCE-9908 represents a novel strategy for targeting leukemia cell/bone marrow microenvironment interaction. Based on these observations, in vivo experiments are ongoing to characterize the efficacy of chemotherapy combined with CTCE-9908.
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Wong, Donald, Walter Korz, Ahmed Merzouk, Hassan Salari, and Bensinger I. William. "A Stromal Cell Derived Factor 1 (SDF-1) Agonist Stimulates Neutrophil Mobilization in Normal Humans after Subcutaneous Injection." Blood 106, no. 11 (November 16, 2005): 4262. http://dx.doi.org/10.1182/blood.v106.11.4262.4262.
Full textAbstract:
Abstract Stromal cell-derived factor-1 (SDF-1) is a well known key signaling molecule in the proliferation, homing, engraftment and expansion of hematopoietic stem cells and leukocytes. CTCE-0214, a peptide produced by rational drug design, is an analog of SDF-1 and agonist of the SDF-1 receptor, CXCR4. In vitro and in vivo models have shown that CTCE-0214 mobilized blood and progenitor cells and enhanced the survival and expansion of cord blood cells (Letters in Drug Design and Discovery.1:126, 2004; Exp Hematol.32:470, 2004; Exp Hematol.32:300, 2004; Stem Cells. In press). In this first phase I clinical trial of CTCE-0214, the safety and tolerability of single doses of CTCE-0214 given subcutaneously to healthy subjects as well as the pharmacokinetic profile and the pharmacodynamic effects were studied. The randomized, double-blinded, placebo-controlled dose-escalation trial enrolled 24 subjects in six dose-escalation groups. Four healthy human subjects in each cohort received either CTCE-0214 or Placebo, randomized in a 3:1 ratio. The starting dose for CTCE-0214 was 0.2 mg/kg. Successive dose cohorts received 0.5 mg/kg, 0.8 mg/kg, 1.5 mg/kg, 2.0 mg/kg, and 3.0 mg/kg. CTCE-0214 was shown to be safe with no serious adverse events reported in any of the dose levels studied. The most common drug related adverse events were injection site pain and erythema which were transient and resolved without intervention. The severity of injection site pain appeared to have an association with the overall quantity of study drug administered. Moderate or severe pain was reported only in subjects who received at least 80 mg of CTCE-0214 per syringe. Dilution across more than one syringe appeared to be effective in reducing injection site pain. Pharmacokinetic analysis of CTCE-0214 plasma concentrations showed that Tmax was reached at 0.25 hours post-administration for all CTCE-0214 treated cohorts. The apparent terminal elimination half-life (t1/2) values were estimated to be 0.41 to 0.32 hours. CTCE-0214 administration was associated with significant dose-dependent increases in total white blood cell and neutrophil counts in treated subjects, peaking at around 6 hours post-injection. In the 3.0 mg/kg arm, the mean difference in neutrophil count from baseline was more than three times the corresponding figure in the Placebo arm. The same trend was apparent in the 2.0 and 1.5 mg/kg arms with a greater than two times and two times increases, respectively. These results suggest that SDF-1 agonists may potentially be used in patients with low neutrophil count receiving chemotherapy with or without the use of G-CSF. The potential for CTCE-0214 to mobilize neutrophils and other blood cells merits serious consideration.