Relevant bibliographies by topics / Cryopreservation

Academic literature on the topic 'Cryopreservation'

Author: Grafiati
Published: 4 June 2021
Last updated: 30 July 2024

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Journal articles on the topic "Cryopreservation"

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Koung Ngeun, Sai, Miki Shimizu, and Masahiro Kaneda. "Characterization of Rabbit Mesenchymal Stem/Stromal Cells after Cryopreservation." Biology 12, no. 10 (October 7, 2023): 1312. http://dx.doi.org/10.3390/biology12101312.

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Adipose tissues (ADPs) are an alternative source for mesenchymal stem/stromal cells (MSCs), given that conventional bone marrow (BM) collection is painful and yields limited cell numbers. As the need for easily accessible MSCs grows, cryopreservation’s role in regenerative medicine is becoming increasingly vital. However, limited research exists on the characteristics and functional properties of rabbit-derived MSCs from various anatomical sources before and after cryopreservation. We examined the effects of cryopreservation using Bambanker. We found that cryopreservation did not adversely affect the morphology, viability, and adipogenic or chondrogenic differentiation abilities of ADP MSCs or BM MSCs. However, there was a notable drop in the proliferation rate and osteogenic differentiation capability of BM MSCs post-cryopreservation. Additionally, after cryopreservation, the surface marker gene expression of CD90 was not evident in ADP MSCs. As for markers, ADIPOQ can serve as an adipogenic marker for ADP MSCs. ACAN and CNMD can act as chondrogenic markers, but these two markers are not as effective post-cryopreservation on ADP MSCs, and osteogenic markers could not be validated. The study highlights that compared to BM MSCs, ADP MSCs retained a higher viability, proliferation rate, and differentiation potential after cryopreservation. As such, in clinical MSC use, we must consider changes in post-cryopreservation cell functions.
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Takeuchi, Hiroki, Mikiko Nishioka, Tadashi Maezawa, Yuko Kitano, Kento Terada-Yoshikawa, Ryota Tachibana, Manabu Kato, et al. "Carboxylated Poly-l-Lysine as a Macromolecular Cryoprotective Agent Enables the Development of Defined and Xeno-Free Human Sperm Cryopreservation Reagents." Cells 10, no. 6 (June 8, 2021): 1435. http://dx.doi.org/10.3390/cells10061435.

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In human sperm cryopreservation, test yolk buffer and human serum albumin have been used as permeating macromolecular-weight cryoprotectants. In clinical reproductive medicine, human serum albumin is frequently used because of low risks of zoonoses and allergic reactions. However, the risk of allogeneic infectious diseases exists, and the supply may be unstable because human serum albumin is derived from human blood. Therefore, the development of xeno-free human sperm cryopreservative reagents that could overcome the aforementioned problems is warranted. We succeeded in developing a new xeno-free and defined sperm cryopreservation reagent containing glycerol, carboxylated poly-l-lysine, and raffinose. The cryopreservation reagent was not significantly different in terms of sperm motility, viability, and DNA fragmentation and was comparable in performance to a commercial cryopreservation reagent containing human serum albumin. Moreover, the addition of saccharides was essential for its long-term storage. These results may help elucidate the unknown function of macromolecular-weight permeating cryoprotective agents.
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Adams, R. Mark, Mary Wang, Ana Maria Crane, Bridgette Brown, Gretchen J. Darlington, and Fred D. Ledley. "Effective Cryopreservation and Long-Term Storage of Primary Human Hepatocytes with Recovery of Viability, Differentiation, and Replicative Potential." Cell Transplantation 4, no. 6 (November 1995): 579–86. http://dx.doi.org/10.1177/096368979500400607.

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Despite reports of successful cryopreservation of primary human hepatocytes, existing methods do not produce sufficient recovery of viable cells to meet the needs of basic research or clinical trials of hepatocellular transplantation. We now describe a protocol for efficient cryopreservation of primary human hepatocytes using University of Wisconsin (UW) solution, fetal bovine serum, and dimethyl sulfoxide (DMSO). This method provides >90% viability of differentiated, primary human hepatocytes 8 mo after cryopreservation as measured by trypan blue exclusion, preserves hepatocyte morphology, liver-specific gene expression α1 antitrypsin), and replication. The effectiveness of UW solution as a cryopreservative agent suggests that metabolic as well as ultrastructural factors may be important in the effective cryopreservation of primary human hepatocytes. The present method represents an effective protocol for cryopreserving differentiated primary human hepatocytes for research. This method may allow characterization and banking of human hepatocytes for clinical applications, including hepatocellular transplantation and hepatic assist devices.
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Crowley, Conor A., William P. W. Smith, K. T. Matthew Seah, Soo-Keat Lim, and Wasim S. Khan. "Cryopreservation of Human Adipose Tissues and Adipose-Derived Stem Cells with DMSO and/or Trehalose: A Systematic Review." Cells 10, no. 7 (July 20, 2021): 1837. http://dx.doi.org/10.3390/cells10071837.

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Adipose tissue senescence is implicated as a major player in obesity- and ageing-related disorders. There is a growing body of research studying relevant mechanisms in age-related diseases, as well as the use of adipose-derived stem cells in regenerative medicine. The cell banking of tissue by utilising cryopreservation would allow for much greater flexibility of use. Dimethyl sulfoxide (DMSO) is the most commonly used cryopreservative agent but is toxic to cells. Trehalose is a sugar synthesised by lower organisms to withstand extreme cold and drought that has been trialled as a cryopreservative agent. To examine the efficacy of trehalose in the cryopreservation of human adipose tissue, we conducted a systematic review of studies that used trehalose for the cryopreservation of human adipose tissues and adipose-derived stem cells. Thirteen articles, including fourteen studies, were included in the final review. All seven studies that examined DMSO and trehalose showed that they could be combined effectively to cryopreserve adipocytes. Although studies that compared nonpermeable trehalose with DMSO found trehalose to be inferior, studies that devised methods to deliver nonpermeable trehalose into the cell found it comparable to DMSO. Trehalose is only comparable to DMSO when methods are devised to introduce it into the cell. There is some evidence to support using trehalose instead of using no cryopreservative agent.
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Khan, Muhammad Shoaib, Mohd Basyaruddin Abdul Rahman, Adamu Abdul Abubakar, Shakeeb Ullah, Humaira A. Khan, Farheen Bhatti, Nayab Batool Rizvi, et al. "AN OVERVIEW OF TISSUE CRYOPRESERVATION'S CONTRIBUTION TO ORGAN CRYOBIOLOGY." Health Sciences Journal 2, no. 1 (December 31, 2023): 8–15. http://dx.doi.org/10.59365/hsj.2(1).2023.54.

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Background: Scientists and physicians have long faced challenges in their quest to identify the most effective methods for cryopreservation of living tissues and organs. While cryopreservation techniques have been used since the 1800s, they are still used for tissue preservation for a comparatively limited time. More advancements in biotechnology for tissue and organ preservation have been made possible by the availability of commercially available liquefied gases. Typical issues such as sub-zero or cell damage and re-crystallization, which ultimately results in tissue loss, impede the usefulness of cryopreservation in the field of medical sciences. However, modern cryobiology advances have provided many alternatives for chilling tissues, organs, gametes, and embryos. As tissue sensitivity to ice is more understood, safer freezing methods are used. Methods: This review discusses how tissue cryopreservation advances biotechnology. The effective banking and transplanting of living tissues and organs using cryopreservation will be a crucial and noteworthy development in the fields of biotechnology and medicine in the future. One of the most crucial elements that will enable future cryobiologists and biotechnology researchers to make enormous strides is the preservation and storage of living tissues from the point of donor removal through transfer and transplantation. Future cryobiologists will find this ancient tale fascinating as it unfolds, thanks to the discovery of anti-freezing proteins and peptides in cryopreservation's active zones.
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Lederman, Lynne. "Cryopreservation." BioTechniques 45, no. 6 (December 2008): 619–21. http://dx.doi.org/10.2144/000112986.

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Baust, John G., Dayong Gao, and John M. Baust. "Cryopreservation." Organogenesis 5, no. 3 (July 2009): 90–96. http://dx.doi.org/10.4161/org.5.3.10021.

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Trounson, A. O. "Cryopreservation." British Medical Bulletin 46, no. 3 (1990): 695–708. http://dx.doi.org/10.1093/oxfordjournals.bmb.a072425.

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Pe, Phyo Phyo Win, Aung Htay Naing, Chang Kil Kim, and Kyeung Il Park. "Antifreeze Protein Improves the Cryopreservation Efficiency of Hosta capitata by Regulating the Genes Involved in the Low-Temperature Tolerance Mechanism." Horticulturae 7, no. 4 (April 15, 2021): 82. http://dx.doi.org/10.3390/horticulturae7040082.

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In this study, whether the addition of antifreeze protein (AFP) to a cryopreservative solution (plant vitrification solution 2 (PVS2)) is more effective in reducing freezing injuries in Hosta capitata than PVS2 alone at different cold exposure times (6, 24, and 48 h) is investigated. The upregulation of C-repeat binding factor 1 (CBF1) and dehydrin 1 (DHN1) in response to low temperature was observed in shoots. Shoots treated with distilled water (dH2O) strongly triggered gene expression 6 h after cold exposure, which was higher than those expressed in PVS2 and PVS2+AFP. However, 24 h after cold exposure, gene expressions detected in dH2O and PVS2 treatments were similar and higher than PVS2 + AFP. The expression was highest in PVS2+AFP when the exposure time was extended to 48 h. Similarly, nitric reductase activities 1 and 2 (Nia1 and Nia2) genes, which are responsible for nitric oxide production, were also upregulated in low-temperature-treated shoots, as observed for CBF1 and DHN1 expression patterns during cold exposure periods. Based on the gene expression patterns, shoots treated with PVS2+AFP were more likely to resist cold stress, which was also associated with the higher cryopreservation efficiency of PVS2+AFP compared to PVS2 alone. This finding suggests that the improvement of cryopreservation efficiency by AFP could be due to the transcriptional regulation of CBF1, DHN1, Nia1, and Nia2, which might reduce freezing injuries during cryopreservation. Thus, AFP could be potentially used as a cryoprotectant in the cryopreservation of rare and commercially important plant germplasm.
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Jovičić, Marija, Eva Chmelíková, and Markéta Sedmíková. "Cryopreservation of boar semen." Czech Journal of Animal Science 65, No. 4 (April 30, 2020): 115–23. http://dx.doi.org/10.17221/47/2020-cjas.

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Sperm cryopreservation is the best technology for long-term storage of the semen. However, the damage of boar spermatozoa by cryopreservation is more severe than in other animal species and a standardized freezing protocol for efficient cryopreservation has not been established yet. Semen quality and freezability vary greatly between breeds as well as between individual boars and even the season. Boar spermatozoa are sensitive to low temperatures; they sustain damage and a high rate of mortality and freezing/thawing the boar semen may strongly impair the sperm function and decrease the semen quality. The freezability of boar semen can be influenced by a cryopreservation procedure, and also by using various additives to freezing and thawing extenders such as antioxidants. In order to obtain acceptable results after thawing the boar semen, it is necessary to combine an optimal amount of additives (glycerol, egg yolk, sugars, antioxidants), cooling and warming velocities.
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Dissertations / Theses on the topic "Cryopreservation"

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Kazem, Rahnuma. "Oocyte cryopreservation." Thesis, University of Aberdeen, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282706.

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A questionnaire based survey was done to assess the views of fertile individuals, infertile individuals, egg donors and recipients towards gamete donation. The survey showed that fertile individuals were significantly less inclined towards the use of donated eggs in research and treatment, compared to infertile individuals. Acceptability of gamete donation was found to be very high in all groups regardless of their fertility, but the majority of individuals, whether fertile or infertile, were opposed to the use of fetal and cadaveric sources of obtaining eggs. The effect of modifications of the freeze-thaw process was investigated in the mouse model. It was seen that slight modifications of the slow freeze protocol affected survival rates and that ultrarapid freezing achieved better survival rates than slow freezing. Human oocyte cryopreservation was performed using a slow freeze-rapid thaw protocol. In total, 34.4% of oocytes survived cryopreservation and these were randomly allocated for fertilisation by conventional in vitro fertilisation (IVF) or intracytoplasmic sperm injection (ICSI). Resulting embryos were spread for chromosomal analysis. ICSI significantly improved the rates of normal fertilisation (43.2% versus 2.7%) compared to IVF (P<0.001). A normal diploid karyotype was achieved by ICSI. These studies show that oocyte donation is acceptable to the majority of both fertile and infertile individuals. Further research is required to improve the methods of oocyte cryopreservation. Once the techniques of cryopreservation have been established, ICSI may successfully be applied to enhance subsequent fertilisation rates.
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Vogel, Martin Joseph. "Proteomic profiling following cryopreservation." Diss., Online access via UMI:, 2004. http://wwwlib.umi.com/dissertations/fullcit/1424168.

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Bradley, Leanne Teresa. "Cryopreservation of bovine spermatozoa." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0016/MQ47313.pdf.

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Beaty, Myron H. "Cryopreservation of eukaryote algae." Thesis, This resource online, 1991. http://scholar.lib.vt.edu/theses/available/etd-07282008-135156/.

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Huang, Yu-Jen. "Cryopreservation of female fertility." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=92216.

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Preservation of female fertility is an important issue today. There are a few effective clinical options for preserving female fertility. Conventional IVF followed by embryo cryopreservation is the only established procedure but is not applicable to all women. Oocyte cryopreservation avoids the ethical and moral concerns related to cryopreservation of embryos but conventional slow freezing methods are associated with low survival rate of oocytes. The main objective of this translational research thesis was to develop an efficient and safe methodology for oocyte cryopreservation that is clinically applicable for female fertility preservation. Specific research objectives were to investigate cryobiology of oocytes in terms of: 1) cryoprotectant (CPA) toxicity effect on oocyte ultra-structures and embryonic developmental potential; 2) Vitrification versus conventional slow freezing of oocytes and their effects of oocyte structures and embryonic developmental potential; 3) Vitrification of embryos using the McGill Cryoleaf and its effect of embryonic development and DNA fragmentation; 4) Clinical efficacy of oocyte vitrification in prospective clinical trials; 5) Effects of oocyte vitrification in terms of the clinical obstetrical and perinatal outcomes; 6) Clinical applications of vitrification of oocytes for preservation of female fertility. The CPA mixture of ethylene glycol (EG) and 1,2-propanediol (PROH) was found to be the most suitable combination for oocyte vitrification, resulting in high embryo development and the least DNA fragmentation. Vitrification of oocyte using the CPA mixture of EG and PROH in combination with the McGill Cryoleaf system is superior to the conventional slow-cooling method, resulting in better preservation of egg ultra-structures and functions. The reduced embryonic development potential of cryopreserved oocyte is related to increased DNA fragmentation and activation of caspase enzymes. Vitrification of human oocytes using the McG
Préservation de la fécondité est un sujet important à ce jour. Il y a peu de traitements effectifs pour préserver la fécondité des femmes. La fécondation in vitro (FIV) conventionnelle suivie par la cryoconservation des embryons est la seule procédure bien établie. Cependant, celle-ci n'est pas possible pour certaines femmes. La cryopréservation des ovocytes évite les problèmes éthiques et moraux reliés à la cryoconservation des embryons. Cependant, les méthodes de congélation lente sont associées à des taux de survie des ovocytes faible. Les objectifs principaux de cette thèse de recherche translationnelle était de développer une méthode efficace et sécuritaire pour la cryopréservation des ovocytes qui est cliniquement applicable pour la préservation de la fécondité des femmes. Les objectifs spécifiques de cette recherche étaient d'étudier la cryobiologie des ovocytes à propos de : 1) l'effet toxique du cryoprotectant sur l'ultra-structures des ovocytes et le potentiel de développement des embryons; 2) la vitrification versus la congélation lente des ovocytes et leur effets sur la structure des ovocytes et le potentiel de développement embryonnaire; 3) la vitrification des embryons en utilisant le McGill Cryoleaf et son effet sur le développement embryonnaire et la fragmentation de l'ADN; 4) l'efficacité clinique de vitrification des ovocytes dans des études prospectives cliniques; 5) les effets de long terme de vitrification des ovocytes au niveau des résultats obstétriques et périnatals; 6) l'application clinique de vitrification des ovocytes pour la préservation de la fécondité des femmes. Le mélange de CPA de l'éthylène glycol (EG) et de 1-2 propanediol (PROH) a été trouvé d'être la combinaison la plus convenable pour la vitrification des ovocytes en donnant des résultats de développement embryonnaire supérieur et à un taux de fragmentation de l'ADN diminué. La vitrification des ovocytes en utilisant l
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Moawad, Adel Reda. "Cryopreservation of ovine oocytes." Thesis, University of Nottingham, 2010. http://eprints.nottingham.ac.uk/27948/.

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Oocyte cryopreservation represents one of the most recent developments in the field of reproductive technologies. However, despite of significant progress, the efficiency of oocyte cryopreservation is still very low. Cryopreservation of mature metaphase II (MIl) oocytes has been reported to induce disorganization of the meiotic spindle and chromosome damage. However, cryopreservation of immature oocyte at germinal vesicle (GV) stage may provide an alternative which avoids these problems. Slow freezing protocols have more recently been replaced by vitrification approaches. In this thesis, recovery, viability and subsequent developmental potential following in vitro fertilisation (IVF), parthenogenetic activation or somatic cell nuclear transfer (SCNT) of ovine oocytes vitrified at GV stage and matured in vitro were studied. Solid surface vitrification (SSV) and cryoloop technologies share the advantages of using a containerless system and small volumes of solution (less than t J.ll) which favours rapid cooling. Maturation, fertilisation, cleavage and blastocyst development were significantly decreased in SSV vitrified oocytes as compared to controls. Following cryoloop vitrification, frequencies of in vitro maturation (43.4 vs 63.2%), oocytes with normal spindle and chromosome configuration (50.0 vs 70.4%) and fertilisation (54.0 vs 74. t %) did not differ significantly between vitrified and control oocytes. Numbers of cleaved embryos that developed to the blastocyst stage following IVM/IVF/IVC did not differ significantly between vitrified and control groups (29.4 vs 45.1 %). In vitro matured ovine oocytes vitrified at GV stage using cryoloop were activated by two different protocols (I) a combination of calcium ionophore (A 23187), cycloheximide and cytochalasin 13 (CA+CHX/CI3), (2) strontium and CB (Sr/Cll). No blastocysts developed in vitrified oocytes activated by CA+CHX/CB; however, 3.8% were obtained following Sr/CI3 activation. Developmental competence of ovinc oocytes vitrified at GV stage and used as cytoplast recipients for SCNT was evaluated. Although the frequencies of cleaved embryos were significantly decreased in vitrified oocytes as compared to control, development to morula and blastocyst stage embryos was not significantly different. No significant differences were observed in total cell numbers, number of apoptotic nuclei as detected by Hoechst and TUNEL assay and proportions of diploid embryos in day 7 blastocysts produced following IVF or seNT of vitrified oocytes as compared to control. Pre-treatment of ovine GV-oocytes with cytochalasin 13 (7.5 J.lglml for 60 min) or demecolcine (0.1 flg/ml for 20 min) prior to vitrification improved frequencies of maturation, fertilisation and subsequent development following IVF or parthenogenetic activation. Caffeine treatment during IVM (10 mM for 6 h) increased the frequencies of blastocyst development in vitrified/thawed GV ovine oocytes. Taken together, these studies suggest that, ovine oocytes vitrified at GV stage can be matured, fertilised and develop in vitro to blastocyst stage embryos. Cryoloop vitrification resulted in higher maturation, fertilisation and subsequent development as compared to SSV. Strontium can be used effectively for parthenogenetic activation of vitrified/thawed ovine GV oocytes. Ovine oocytes vitrified at GV stage can be used effectively as cytoplast recipients for SCNT.
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Gryshkov, O., V. Mutsenko, M. Tymkovych, D. Tarusin, V. Sirotinskaya, I. Braslavsky, О. Г. Аврунін, and B. Glasmacher. "Advances in cryopreservation of alginate-encapsulated stem cells and analysis of cryopreservation outcome." Thesis, Інститут проблем кріобіології та кріомедицини НАН України, 2018. http://openarchive.nure.ua/handle/document/8338.

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Chan, Gene Yel. "Cryopreservation of porcine heart valves." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ60420.pdf.

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Marsland, T. P. "Dielectric measurements for organ cryopreservation." Thesis, University of Cambridge, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373270.

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Pandolfi, Susan M. "Cryopreservation of microencapsulated bovine spermatozoa." Thesis, This resource online, 1996. http://scholar.lib.vt.edu/theses/available/etd-11012008-063722/.

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Books on the topic "Cryopreservation"

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Chian, Ri-Cheng, and Patrick Quinn, eds. Fertility Cryopreservation. Cambridge: Cambridge University Press, 2009. http://dx.doi.org/10.1017/cbo9780511730207.

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Simione, Frank P. Cryopreservation manual. Rochester, N.Y: Nalge, 1988.

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Ri-Cheng, Chian, and Quinn Patrick 1946-, eds. Fertility cryopreservation. Cambridge: Cambridge University Press, 2010.

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Rajasekharan, P. E., and M. R. Rohini, eds. Pollen Cryopreservation Protocols. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2843-0.

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Betsy, Judith, and Stephen Kumar, eds. Cryopreservation of Fish Gametes. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-4025-7.

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R, Tiersch Terrence, Mazik Patricia M, and World Aquaculture Society, eds. Cryopreservation in aquatic species. Baton Rouge, La: World Aquaculture Society, 2000.

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Cloud, Joseph G. Cryopreservation of salmonid sperm. Moscow, Idaho: Dept. of Biological Sciences, University of Idaho, 1997.

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Panis, Bart. Cryopreservation of Musa germplasm. Montpellier: International Network for the Improvement of Banana and Plantain, 2001.

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Nagy, Zsolt Peter, Alex C. Varghese, and Ashok Agarwal, eds. Cryopreservation in Assisted Reproduction. Cham: Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-58214-1.

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Day, John G., and Mark R. McLellan. Cryopreservation and Freeze-Drying Protocols. New Jersey: Humana Press, 1995. http://dx.doi.org/10.1385/0896032965.

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Book chapters on the topic "Cryopreservation"

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Pegg, David. "Cryopreservation." In Essentials of Tissue Banking, 109–21. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9142-0_8.

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Day, John G., Keith C. Harding, Jayanthi Nadarajan, and Erica E. Benson. "Cryopreservation." In Springer Protocols Handbooks, 917–47. Totowa, NJ: Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-375-6_52.

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Vendrame, Wagner A. "Cryopreservation." In Springer Protocols Handbooks, 283–302. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7771-0_15.

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Towill, L. E. "Cryopreservation." In In Vitro Methods for Conservation of Plant Genetic Resources, 41–70. Dordrecht: Springer Netherlands, 1991. http://dx.doi.org/10.1007/978-94-011-3072-1_3.

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Engelmann, Florent, and Stéphane Dussert. "Cryopreservation." In Conservation of Tropical Plant Species, 107–19. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-3776-5_6.

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Pintado, B., and J. Hourcade. "Cryopreservation." In Springer Protocols Handbooks, 577–99. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-662-45763-4_23.

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Patel, Sandesh K., and Sonu T. Lukose. "Cryopreservation." In Atlas of Assisted Reproductive Technologies, 253–62. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-99-0020-6_16.

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Sharma, Sanjeev Kumar. "Cryopreservation." In Basics of Hematopoietic Stem Cell Transplant, 169–80. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-5802-1_15.

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Borini, Andrea, and Veronica Bianchi. "Oocyte Cryopreservation." In Fertility Preservation in Females, 329–34. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-5617-9_20.

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Borini, Andrea, and Veronica Bianchi. "Oocyte Cryopreservation." In Fertility Preservation in Females, 111–32. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-5617-9_8.

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Conference papers on the topic "Cryopreservation"

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Rajamohan, Arun. "Climate, insects, and cryopreservation." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94912.

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Karlsson, J., and H. Udaykumar. "On cryopreservation and microgravity." In 40th AIAA Aerospace Sciences Meeting & Exhibit. Reston, Virigina: American Institute of Aeronautics and Astronautics, 2002. http://dx.doi.org/10.2514/6.2002-328.

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Diller, Kenneth R., and Robson Cintra de Freitas. "Cryopreservation of Multicellular Tissue." In ASME 1996 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1996. http://dx.doi.org/10.1115/imece1996-1164.

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Abstract The cryopreservation of living cells and tissues can provide a key source of materials for applications in tissue engineering as well as for direct transplantation to recipients. Successful recovery of living tissues from long term exposure to the deep subzero temperatures necessary to create a state of suspended animation requires careful and knowledgeable design of the processing protocol. This paper presents an analysis of the design issues which must be considered in the development of cryopreservation techniques for organized tissues and a brief summary of our work with a specific example, the pancreas islet of Langerhans.
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Rabin, Yoed, Justin S. G. Feig, Alexander C. Williams, Christopher C. Lin, and Chandrajit Thaokar. "Cryomacroscopy in 3D: A Device Prototype for the Study of Cryopreservation." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80527.

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This study presents a new device prototype for visualization of physical effects associated with large-scale cryopreservation—the preservation of tissues at very low temperatures. Cryopreservation represents the only method for long-term preservation of biomaterials. While techniques for cryopreservation of single cells and small tissue structures are well established, cryopreservation techniques for bulky tissues and organs are still at the developmental stage. Critical to the success of cryopreservation is the control of ice formation—the cornerstone of cryoinjury. One of the most promising techniques for large-scale cryopreservation is known as vitrification, where the crystal phase is suppressed, and the biological material is trapped in a glassy-like state (vitreous in Latin means glassy) [1].
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Feig, Justin S. G., Alexander C. Williams, Christopher C. Lin, and Yoed Rabin. "Developing the cryomacroscope for cryopreservation applications." In 2012 38th Annual Northeast Bioengineering Conference (NEBEC). IEEE, 2012. http://dx.doi.org/10.1109/nebc.2012.6207019.

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Olmo, A., B. Buzón, A. Yúfera, and R. Risco. "Bioimpedance monitoring for cryopreservation process control." In 2010 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2010). IEEE, 2010. http://dx.doi.org/10.1109/iembs.2010.5627104.

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TKACHEV, Alexander V., Olga L. TKACHEVA, Lyudmila V. GAZZAVI-ROGOZINA, Tatyana V. ZUBOVA, Oksana V. SMOLOVSKAYA, and Vladimir A. PLESHKOV. "Modern Technology of Poultry Semen Cryopreservation." In IV International Scientific and Practical Conference "Modern S&T Equipments and Problems in Agriculture". Sibac, 2020. http://dx.doi.org/10.32743/kuz.mepa.2020.235-244.

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Jianying Wu, Tiejun Hu, Yang Liu, Bo Jin, Guanyu Che, Bin Guo, Zhanpeng Yue, Ziyi Li, and Xueming Zhang. "Cryopreservation of adult bovine testicular tissue." In 2011 International Conference on Remote Sensing, Environment and Transportation Engineering (RSETE). IEEE, 2011. http://dx.doi.org/10.1109/rsete.2011.5964048.

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Baust, John M., Robert G. Van Buskirk, and John G. Baust. "Cryopreservation of an Engineered Skin Equivalent: The Apoptosis Paradigm." In ASME 1999 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/imece1999-0586.

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Abstract The emergence of the tissue engineering sciences has led to an immediate need for cryopreservation protocols compatible with cell survival, cell-matrix binding and “scaffold” integrity. While numerous problems exist related to directional restrictions in cryoprotectant transport in tissue models, recent findings have demonstrated that the induction of specific molecular events, especially gene regulated cell death (apoptosis) is of significance. Accordingly, we report on a new strategy for the successful cryopreservation of a human skin equivalent. The integration of an intracellular-type cryopreservation solution containing dimethyl sulfoxide with and without apoptotic inhibitors provides a successful model for tissue preservation. With optimized formulations, post-thaw improvement of viability upwards of three-fold has been demonstrated when compared with traditional preservation methodologies. We conclude that 1) the use of an intracellular-type cryopreservation medium, Hypothermosol®, can improve post-thaw tissue construct integrity and cell viability, and 2) the inclusion of apoptotic inhibitors significantly improves cryopreservation outcome.
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Sarangi, S. K., and K. Pramanik. "Cryopreservation in Tissue Engineering: Challenges & Prospects." In 2010 Advanced Technologies for Enhancing Quality of Life (ATEQUAL). IEEE, 2010. http://dx.doi.org/10.1109/atequal.2010.8.

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Reports on the topic "Cryopreservation"

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SAMBANIS, ATHANASSIOS. Final Report on Cryopreservation of Biological Tissues. Office of Scientific and Technical Information (OSTI), June 2001. http://dx.doi.org/10.2172/782715.

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Kulus, Dariusz, and Alicja Tymoszuk. Application of nanoparticles in cryopreservation of plant tissues. Peeref, April 2023. http://dx.doi.org/10.54985/peeref.2304p9420331.

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Beniers, J. E., S. Bras, and A. K. Werf. Cryopreservation of Ulva spp. and Saccharina latissima: : optimised protocols of cryopreservation, recovery and (long-term) regrowth of Ulva spp. and Saccharina latissima gametophytes and sporophytes. Wageningen: Stichting Wageningen Research, Wageningen Plant Research, Business Unit Agrosystems research, 2023. http://dx.doi.org/10.18174/640169.

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Barnard, M. R., H. MacGregor, A. D. Michelson, and C. R. Valeri. Effects of Liquid Storage and Cryopreservation on Platelet Surface Glycoproteins, Light Scatter, and Procoagulant Activity. Fort Belvoir, VA: Defense Technical Information Center, May 1996. http://dx.doi.org/10.21236/ada360295.

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Barnard, M. R., H. Macgregor, A. D. Michelson, and C. R. Valeri. Effects of White Cell Filtration, ACD Concentration and Rotation During Collection, Storage and Cryopreservation of Platelet Concentrates. Fort Belvoir, VA: Defense Technical Information Center, May 1997. http://dx.doi.org/10.21236/ada360226.

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Martín-López, María, Cristina Rosell-Valle, Blanca Arribas-Arribas, Rafael Campos-Cuerva, Inma Piudo, Isidora Ranchal, Beatriz Fernandez-Muñoz, Miguel Alaminos, Gloria Carmona-Sánchez, and Mónica Santos-González. Evaluation of cryopreservation solutions based on human platelet lysate for bioengineered bissues aimed for advanced therapy treatments. Peeref, June 2023. http://dx.doi.org/10.54985/peeref.2306p4363615.

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Faurot, Dave, Paul A. Kucera, and Robyn D. Armstrong. Cryopreservation of Adult Male Spring and Summer Chinook Salmon Gametes in the Snake River Basin, 1997 Annual Report. Office of Scientific and Technical Information (OSTI), June 1998. http://dx.doi.org/10.2172/14575.

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Andreeva, Madlena, Nikola Metodiev, Paulina Taushanova, and Rossen Stefanov. Influence of Cryopreservation on the Velocity Parameters of Spermatozoa from Breeds of Lacaune and Ile De France Sheep. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, December 2018. http://dx.doi.org/10.7546/crabs.2018.12.18.

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Hansen, Peter J., and Amir Arav. Embryo transfer as a tool for improving fertility of heat-stressed dairy cattle. United States Department of Agriculture, September 2007. http://dx.doi.org/10.32747/2007.7587730.bard.

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The overall objective of the current proposal is to develop procedures to improve the pregnancy rate achieved following transfer of fresh or cryopreserved embryos produced in the laboratory into heat-stress recipients. The overall hypothesis is that pregnancy rate in heat-stressed lactating cows can be improved by use of embryo transfer and that additional gains in pregnancy rate can be achieved through development of procedures to cryopreserve embryos, select embryos most likely to establish and maintain pregnancy after transfer, and to enhance embryo competence for post-transfer survival through manipulation of culture conditions. The original specific objectives were to 1) optimize procedures for cryopreservation (Israel/US), 2) develop procedures for identifying embryos with the greatest potential for development and survival using the remote monitoring system called EmbryoGuard (Israel), 3) perform field trials to test the efficacy of cryopreservation and the EmbryoGuard selection system for improving pregnancy rates in heat-stressed, lactating cows (US/Israel), 4) test whether selection of fresh or frozen-thawed blastocysts based on measurement of group II caspase activity is an effective means of increasing survival after cryopreservation and post-transfer pregnancy rate (US), and 5) identify genes in blastocysts induced by insulin-like growth factor-1 (IGF-1) (US). In addition to these objectives, additional work was carried out to determine additional cellular determinants of embryonic resistance to heat shock. There were several major achievements. Results of one experiment indicated that survival of embryos to freezing could be improved by treating embryos with cytochalasin B to disrupt the cytoskeleton. An additional improvement in the efficacy of embryo transfer for achieving pregnancy in heat-stressed cows follows from the finding that IGF-1 can improve post-transfer survival of in vitro produced embryos in the summer but not winter. Expression of several genes in the blastocyst was regulated by IGF-1 including IGF binding protein-3, desmocollin II, Na/K ATPase, Bax, heat shock protein 70 and IGF-1 receptor. These genes are likely candidates 1) for developing assays for selection of embryos for transfer and 2) as marker genes for improving culture conditions for embryo production. The fact that IGF-1 improved survival of embryos in heat-stressed recipients only is consistent with the hypothesis that IGF-1 confers cellular thermotolerance to bovine embryos. Other experiments confirmed this action of IGF-1. One action of IGF-1, the ability to block heat-shock induced apoptosis, was shown to be mediated through activation of the phosphatidylinositol 3-kinase pathway. Other cellular determinants of resistance of embryos to elevated temperature were identified including redox status of the embryo and the ceramide signaling pathway. Developmental changes in embryonic apoptosis responses in response to heat shock were described and found to include alterations in the capacity of the embryo to undergo caspase-9 and caspase-3 activation as well as events downstream from caspase-3 activation. With the exception of IGF-1, other possible treatments to improve pregnancy rate to embryo transfer were not effective including selection of embryos for caspase activity, treatment of recipients with GnRH.and bilateral transfer of twin embryos. In conclusion, accomplishments achieved during the grant period have resulted in methods for improving post-transfer survival of in vitro produced embryos transferred into heat-stressed cows and have lead to additional avenues for research to increase embryo resistance to elevated temperature and improve survival to cryopreservation. In addition, embryo transfer of vitrified IVF embryos increased significantly the pregnancy rate in repeated breeder cows.
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Browdy, Craig, and Esther Lubzens. Cryopreservation of Penaeid Shrimp Embryos: Development of a Germplasm Cryo-Bank for Preservation of High Health and Genetically Improved Stocks. United States Department of Agriculture, August 2002. http://dx.doi.org/10.32747/2002.7695849.bard.

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The objectives of the project were to develop a successful protocol for cryopreservation of penaeid germ plasm in order to preserve a pathogen-free broodstock nucleus for commercial exploitation of marine shrimp in aquaculture. The critical parameters to be characterized in the project were: 1. Determination of chill sensitivity and chill tolerant embryonic stages, including a full description and time course study of embryonic developmental stages. 2. Development of protocols for loading and removal of cryoprotectant agents (CPAs) from embryos; determination of optimal concentrations and duration of loading. 3. Characterization of the toxicity of the selected CP As and 4. Establishing optimal cooling and thawing procedures. Studies were performed on two penaeid species: Litopenaeus vannamei (in the USA) and P. semisulcatus (in Israel). The effect of incubation temperature on embryonic development rate and hatching success was studied in L. vannamei, showing that spawns maybe maintained at temperatures ranging from 24°C to 30°C, without compromising hatchability. Embryonic development extends from 12 hr to 19 hr at 30°C and 24°C, respectively. Studies showed that advanced embryonic developmental stages were chill tolerant in the two studied species, but P. semisulcatus could better endure lower temperatures than L. vannamei. A large number of experiments were performed to determine the optimal CP As, their concentration and duration of loading. Permeating (e.g. glycerol, methanol, DMSO, 1,2- propanediol, ethylene glycol, glucose) and non-permeating CPAs (sucrose, PVP, polyethylene glycol) were tested and several combinations of permeating and non-permeating CP As, on fertilized eggs (embryos), nauplii and protozoeae. In general, nauplii tolerated higher CPA concentrations than eggs and nauplii were also more permeable to radiolabeled methanol. Chlorine treatment intended to remove the chitinous envelop from eggs, did not increase dramatically the permeation of radiolabled methanol into eggs. Cooling eggs, nauplii or protozoeae to cryogenic temperatures, by either vitrification or slow cooling protocols, did not result in full survival of thawed samples, despite exhaustive attempts testing various protocols and CP As. Results seemed more encouraging in freezing of nauplii in comparison to eggs or protozoeae. Successful preliminary results in cryopreservation of spermatozoa of P. vannamei, will facilitate preservation of genetic specific to some extent.
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