Journal articles on the topic 'CRISPR, Cas9, genome editing, gRNA'
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Jo, Areum, Sangwoo Ham, Gum Hwa Lee, Yun-Il Lee, SangSeong Kim, Yun-Song Lee, Joo-Ho Shin, and Yunjong Lee. "Efficient Mitochondrial Genome Editing by CRISPR/Cas9." BioMed Research International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/305716.
Full textXie, Kabin, Bastian Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences 112, no. 11 (March 2, 2015): 3570–75. http://dx.doi.org/10.1073/pnas.1420294112.
Full textMekler, Vladimir, Konstantin Kuznedelov, and Konstantin Severinov. "Quantification of the affinities of CRISPR–Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences." Journal of Biological Chemistry 295, no. 19 (April 1, 2020): 6509–17. http://dx.doi.org/10.1074/jbc.ra119.012239.
Full textBruegmann, Tobias, Khira Deecke, and Matthias Fladung. "Evaluating the Efficiency of gRNAs in CRISPR/Cas9 Mediated Genome Editing in Poplars." International Journal of Molecular Sciences 20, no. 15 (July 24, 2019): 3623. http://dx.doi.org/10.3390/ijms20153623.
Full textWardhani, Bantari W. K., Meidi U. Puteri, Yukihide Watanabe, Melva Louisa, Rianto Setiabudy, and Mitsuyasu Kato. "TMEPAI genome editing in triple negative breast cancer cells." Medical Journal of Indonesia 26, no. 1 (May 16, 2017): 14–8. http://dx.doi.org/10.13181/mji.v26i1.1871.
Full textKong, Qihui, Jie Li, Shoudong Wang, Xianzhong Feng, and Huixia Shou. "Combination of Hairy Root and Whole-Plant Transformation Protocols to Achieve Efficient CRISPR/Cas9 Genome Editing in Soybean." Plants 12, no. 5 (February 23, 2023): 1017. http://dx.doi.org/10.3390/plants12051017.
Full textJameel, Mohd Rizwan. "From design to validation of CRISPR/gRNA primers towards genome editing." Bioinformation 18, no. 5 (May 31, 2022): 471–77. http://dx.doi.org/10.6026/97320630018471.
Full textJung, Soo Bin, Chae young Lee, Kwang-Ho Lee, Kyu Heo, and Si Ho Choi. "A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency." Nucleic Acids Research 49, no. 15 (June 4, 2021): e85-e85. http://dx.doi.org/10.1093/nar/gkab467.
Full textForeman, Hui-Chen Chang, Varvara Kirillov, Gabrielle Paniccia, Demetra Catalano, Trevor Andrunik, Swati Gupta, Laurie T. Krug, and Yue Zhang. "RNA-guided gene editing of the murine gammaherpesvirus 68 genome reduces infectious virus production." PLOS ONE 16, no. 6 (June 4, 2021): e0252313. http://dx.doi.org/10.1371/journal.pone.0252313.
Full textYoo, Byung-Chun, Narendra S. Yadav, Emil M. Orozco, and Hajime Sakai. "Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts." PeerJ 8 (January 6, 2020): e8362. http://dx.doi.org/10.7717/peerj.8362.
Full textCheng, Hao, Feng Zhang, and Yang Ding. "CRISPR/Cas9 Delivery System Engineering for Genome Editing in Therapeutic Applications." Pharmaceutics 13, no. 10 (October 9, 2021): 1649. http://dx.doi.org/10.3390/pharmaceutics13101649.
Full textSant’Ana, Rodrigo Ribeiro Arnt, Clarissa Alves Caprestano, Rubens Onofre Nodari, and Sarah Zanon Agapito-Tenfen. "PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts." Genes 11, no. 9 (September 2, 2020): 1029. http://dx.doi.org/10.3390/genes11091029.
Full textBhagwat, Aditya M., Johannes Graumann, Rene Wiegandt, Mette Bentsen, Jordan Welker, Carsten Kuenne, Jens Preussner, Thomas Braun, and Mario Looso. "multicrispr: gRNA design for prime editing and parallel targeting of thousands of targets." Life Science Alliance 3, no. 11 (September 9, 2020): e202000757. http://dx.doi.org/10.26508/lsa.202000757.
Full textJi, Jie, Chunyang Zhang, Zhongfeng Sun, Longlong Wang, Deqiang Duanmu, and Qiuling Fan. "Genome Editing in Cowpea Vigna unguiculata Using CRISPR-Cas9." International Journal of Molecular Sciences 20, no. 10 (May 19, 2019): 2471. http://dx.doi.org/10.3390/ijms20102471.
Full textEasmin, Farhana, Naim Hassan, Yu Sasano, Keisuke Ekino, Hisataka Taguchi, and Satoshi Harashima. "gRNA-transient expression system for simplified gRNA delivery in CRISPR/Cas9 genome editing." Journal of Bioscience and Bioengineering 128, no. 3 (September 2019): 373–78. http://dx.doi.org/10.1016/j.jbiosc.2019.02.009.
Full textAlok, Anshu, Hanny Chauhan, Santosh Kumar Upadhyay, Ashutosh Pandey, Jitendra Kumar, and Kashmir Singh. "Compendium of Plant-Specific CRISPR Vectors and Their Technical Advantages." Life 11, no. 10 (September 28, 2021): 1021. http://dx.doi.org/10.3390/life11101021.
Full textGasanov, Eugene V., Justyna Jędrychowska, Michal Pastor, Malgorzata Wiweger, Axel Methner, and Vladimir P. Korzh. "An improved method for precise genome editing in zebrafish using CRISPR-Cas9 technique." Molecular Biology Reports 48, no. 2 (January 22, 2021): 1951–57. http://dx.doi.org/10.1007/s11033-020-06125-8.
Full textYamamoto, Akihiro, Takashi Ishida, Mika Yoshimura, Yuri Kimura, and Shinichiro Sawa. "Developing Heritable Mutations in Arabidopsis thaliana Using a Modified CRISPR/Cas9 Toolkit Comprising PAM-Altered Cas9 Variants and gRNAs." Plant and Cell Physiology 60, no. 10 (June 14, 2019): 2255–62. http://dx.doi.org/10.1093/pcp/pcz118.
Full textLessard, Samuel, Laurent Francioli, Jessica Alfoldi, Jean-Claude Tardif, Patrick T. Ellinor, Daniel G. MacArthur, Guillaume Lettre, Stuart H. Orkin, and Matthew C. Canver. "Human genetic variation alters CRISPR-Cas9 on- and off-targeting specificity at therapeutically implicated loci." Proceedings of the National Academy of Sciences 114, no. 52 (December 11, 2017): E11257—E11266. http://dx.doi.org/10.1073/pnas.1714640114.
Full textWestbrook, Adam W., Murray Moo-Young, and C. Perry Chou. "Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis." Applied and Environmental Microbiology 82, no. 16 (June 3, 2016): 4876–95. http://dx.doi.org/10.1128/aem.01159-16.
Full textGhoshal, Basudev, Brandon Vong, Colette L. Picard, Suhua Feng, Janet M. Tam, and Steven E. Jacobsen. "A viral guide RNA delivery system for CRISPR-based transcriptional activation and heritable targeted DNA demethylation in Arabidopsis thaliana." PLOS Genetics 16, no. 12 (December 14, 2020): e1008983. http://dx.doi.org/10.1371/journal.pgen.1008983.
Full textAiba, Wataru, Takamitsu Amai, Mitsuyoshi Ueda, and Kouichi Kuroda. "Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases." Biomolecules 12, no. 11 (November 3, 2022): 1621. http://dx.doi.org/10.3390/biom12111621.
Full textTraxler, Elizabeth, Yu Yao, Chunliang Li, Jeremy Grevet, Peng Huang, Shaela Wright, Gerd A. Blobel, and Mitchell J. Weiss. "Genome Editing Recreates Hereditary Persistence of Fetal Hemoglobin in Primary Human Erythroblasts." Blood 126, no. 23 (December 3, 2015): 640. http://dx.doi.org/10.1182/blood.v126.23.640.640.
Full textGao, Zongliang, Minghui Fan, Atze T. Das, Elena Herrera-Carrillo, and Ben Berkhout. "Extinction of all infectious HIV in cell culture by the CRISPR-Cas12a system with only a single crRNA." Nucleic Acids Research 48, no. 10 (April 13, 2020): 5527–39. http://dx.doi.org/10.1093/nar/gkaa226.
Full textBinyameen, Barkha, Zulqurnain Khan, Sultan Habibullah Khan, Aftab Ahmad, Nayla Munawar, Muhammad Salman Mubarik, Hasan Riaz, et al. "Using Multiplexed CRISPR/Cas9 for Suppression of Cotton Leaf Curl Virus." International Journal of Molecular Sciences 22, no. 22 (November 21, 2021): 12543. http://dx.doi.org/10.3390/ijms222212543.
Full textRoberson, Elisha D. "Identification of high-efficiency 3′GG gRNA motifs in indexed FASTA files with ngg2." PeerJ Computer Science 1 (November 18, 2015): e33. http://dx.doi.org/10.7717/peerj-cs.33.
Full textANURAGI, HIRDAYESH, AMBATI SRIJAN, and BHARAT TAINDU JAIN. "RNA-guided multiplex genome engineering using cas9 nucleases for crop improvement: A review." Indian Journal of Agricultural Sciences 88, no. 12 (December 11, 2018): 1811–17. http://dx.doi.org/10.56093/ijas.v88i12.85371.
Full textOkada, Keita, Kanae Aoki, Teruyuki Tabei, Kota Sugio, Katsunori Imai, Yuki Bonkohara, and Yusuke Kamachi. "Key sequence features of CRISPR RNA for dual-guide CRISPR-Cas9 ribonucleoprotein complexes assembled with wild-type or HiFi Cas9." Nucleic Acids Research 50, no. 5 (February 15, 2022): 2854–71. http://dx.doi.org/10.1093/nar/gkac100.
Full textLouie, Wilson, Max W. Shen, Zakir Tahiry, Sophia Zhang, Daniel Worstell, Christopher A. Cassa, Richard I. Sherwood, and David K. Gifford. "Machine learning based CRISPR gRNA design for therapeutic exon skipping." PLOS Computational Biology 17, no. 1 (January 8, 2021): e1008605. http://dx.doi.org/10.1371/journal.pcbi.1008605.
Full textLouie, Wilson, Max W. Shen, Zakir Tahiry, Sophia Zhang, Daniel Worstell, Christopher A. Cassa, Richard I. Sherwood, and David K. Gifford. "Machine learning based CRISPR gRNA design for therapeutic exon skipping." PLOS Computational Biology 17, no. 1 (January 8, 2021): e1008605. http://dx.doi.org/10.1371/journal.pcbi.1008605.
Full textKato, Yuya, Hirotaka Tabata, Kumiko Sato, Mariko Nakamura, Izumu Saito, and Tomoko Nakanishi. "Adenovirus Vectors Expressing Eight Multiplex Guide RNAs of CRISPR/Cas9 Efficiently Disrupted Diverse Hepatitis B Virus Gene Derived from Heterogeneous Patient." International Journal of Molecular Sciences 22, no. 19 (September 29, 2021): 10570. http://dx.doi.org/10.3390/ijms221910570.
Full textLi, Qi, Bingbing Sun, Jun Chen, Yiwen Zhang, Yu Jiang, and Sheng Yang. "A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli." Acta Biochimica et Biophysica Sinica 53, no. 5 (March 25, 2021): 620–27. http://dx.doi.org/10.1093/abbs/gmab036.
Full textArakawa, Hiroshi. "A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism." Science Advances 2, no. 8 (August 2016): e1600699. http://dx.doi.org/10.1126/sciadv.1600699.
Full textNurkhasanah, Hidayati, Suharsono Suharsono, and Tri Joko Santoso. "Construction and Introduction of CRISPR/Cas9-gRNA-Vinv for Editing A Gene Controlling Cold-Induced Sweetening [CIS] Character on Potato." Jurnal AgroBiogen 18, no. 1 (August 15, 2022): 21. http://dx.doi.org/10.21082/jbio.v18n1.2022.p21-32.
Full textZhang, Ping, Yu Wang, Chenxi Li, Xiaoyu Ma, Lan Ma, and Xudong Zhu. "Simplified All-In-One CRISPR-Cas9 Construction for Efficient Genome Editing in Cryptococcus Species." Journal of Fungi 7, no. 7 (June 24, 2021): 505. http://dx.doi.org/10.3390/jof7070505.
Full textAguilar, Rhiannon R., Zih-Jie Shen, and Jessica K. Tyler. "A Simple, Improved Method for Scarless Genome Editing of Budding Yeast Using CRISPR-Cas9." Methods and Protocols 5, no. 5 (October 4, 2022): 79. http://dx.doi.org/10.3390/mps5050079.
Full textMubarok, Muhamad Husni, Atmitri Sisharmini, Aniversari Apriana, Tri Joko Santoso, and Suharsono Suharsono. "(CRISPR/Cas9 Cassette Construction for OsARF2 Gene Editing and Development of Transgenic Rice Nipponbare Containing CRISPR/Cas9-OsARF2." Jurnal AgroBiogen 18, no. 1 (October 4, 2022): 45. http://dx.doi.org/10.21082/jbio.v18n1.2022.p45-56.
Full textPark, So Hyun, Ciaran M. Lee, Harshavardhan Deshmukh, and Gang Bao. "Therapeutic Crispr/Cas9 Genome Editing for Treating Sickle Cell Disease." Blood 128, no. 22 (December 2, 2016): 4703. http://dx.doi.org/10.1182/blood.v128.22.4703.4703.
Full textAtmanli, Ayhan, Andreas C. Chai, Miao Cui, Zhaoning Wang, Takahiko Nishiyama, Rhonda Bassel-Duby, and Eric N. Olson. "Cardiac Myoediting Attenuates Cardiac Abnormalities in Human and Mouse Models of Duchenne Muscular Dystrophy." Circulation Research 129, no. 6 (September 3, 2021): 602–16. http://dx.doi.org/10.1161/circresaha.121.319579.
Full textSong, Letian, Jean-Paul Ouedraogo, Magdalena Kolbusz, Thi Truc Minh Nguyen, and Adrian Tsang. "Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger." PLOS ONE 13, no. 8 (August 24, 2018): e0202868. http://dx.doi.org/10.1371/journal.pone.0202868.
Full textDhokane, Dhananjay, Bhaskar Bhadra, and Santanu Dasgupta. "CRISPR based targeted genome editing of Chlamydomonas reinhardtii using programmed Cas9-gRNA ribonucleoprotein." Molecular Biology Reports 47, no. 11 (October 19, 2020): 8747–55. http://dx.doi.org/10.1007/s11033-020-05922-5.
Full textKhan, Sikandar. "Recent Advancement and Innovations in CRISPR/Cas and CRISPR Related Technologies: A review." Biotechnology and Bioprocessing 2, no. 5 (June 24, 2021): 01–12. http://dx.doi.org/10.31579/2766-2314/042.
Full textMoniruzzaman, M., Yun Zhong, Zhifeng Huang, and Guangyan Zhong. "Having a Same Type IIS Enzyme’s Restriction Site on Guide RNA Sequence Does Not Affect Golden Gate (GG) Cloning and Subsequent CRISPR/Cas Mutagenesis." International Journal of Molecular Sciences 23, no. 9 (April 28, 2022): 4889. http://dx.doi.org/10.3390/ijms23094889.
Full textPrasanth, Jagannadham, and Thirugnanavel Anbalagan. "CRISPR-based genome editing: Catching impossibles for citrus improvements." International Journal of Agricultural and Applied Sciences 2, no. 1 (June 30, 2021): 24–29. http://dx.doi.org/10.52804/ijaas2021.212.
Full textKurniawati, Devi Ayu, NFN Suharsono, and Tri Joko Santoso. "Editing of PCNA Gene by CRISPR/Cas9 Technology to Improve the Red Chili Resistance to Yellow Leaf Curl Disease." Jurnal AgroBiogen 16, no. 2 (December 11, 2020): 79. http://dx.doi.org/10.21082/jbio.v16n2.2020.p79-88.
Full textTang, Ning, Yumei Xia, Yijie Zhan, Junhao Dan, Mulan Yu, Xiaolan Bu, and Mengliang Cao. "Improvement of Chloroplast Transformation Using CRISPR/Cas9." Journal of Biobased Materials and Bioenergy 14, no. 3 (June 1, 2020): 401–7. http://dx.doi.org/10.1166/jbmb.2020.1970.
Full textYang, Yue, Donghua Li, Fen Wan, Bohong Chen, Guanglan Wu, Feng Li, Yanliang Ren, Puping Liang, Jian Wan, and Zhou Songyang. "Identification and Analysis of Small Molecule Inhibitors of CRISPR-Cas9 in Human Cells." Cells 11, no. 22 (November 11, 2022): 3574. http://dx.doi.org/10.3390/cells11223574.
Full textPark, So Hyun Julie Park, Mingming Cao, Yankai Zhang, Vivien A. Sheehan, and Gang Bao. "CRISPR/Cas9 Editing Induces High Rates of Unintended Large Gene Modifications in HSPCs from Patients with Sickle Cell Disease." Blood 138, Supplement 1 (November 5, 2021): 3969. http://dx.doi.org/10.1182/blood-2021-150739.
Full textRiesenberg, Stephan, Nelly Helmbrecht, Philipp Kanis, Tomislav Maricic, and Svante Pääbo. "Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage." Nature Communications 13, no. 1 (January 25, 2022). http://dx.doi.org/10.1038/s41467-022-28137-7.
Full textAllen, Daniel, Michael Rosenberg, and Ayal Hendel. "Using Synthetically Engineered Guide RNAs to Enhance CRISPR Genome Editing Systems in Mammalian Cells." Frontiers in Genome Editing 2 (January 28, 2021). http://dx.doi.org/10.3389/fgeed.2020.617910.
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