Academic literature on the topic 'CRISPR, Cas9, genome editing, gRNA'
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Journal articles on the topic "CRISPR, Cas9, genome editing, gRNA"
Jo, Areum, Sangwoo Ham, Gum Hwa Lee, Yun-Il Lee, SangSeong Kim, Yun-Song Lee, Joo-Ho Shin, and Yunjong Lee. "Efficient Mitochondrial Genome Editing by CRISPR/Cas9." BioMed Research International 2015 (2015): 1–10. http://dx.doi.org/10.1155/2015/305716.
Full textXie, Kabin, Bastian Minkenberg, and Yinong Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences 112, no. 11 (March 2, 2015): 3570–75. http://dx.doi.org/10.1073/pnas.1420294112.
Full textMekler, Vladimir, Konstantin Kuznedelov, and Konstantin Severinov. "Quantification of the affinities of CRISPR–Cas9 nucleases for cognate protospacer adjacent motif (PAM) sequences." Journal of Biological Chemistry 295, no. 19 (April 1, 2020): 6509–17. http://dx.doi.org/10.1074/jbc.ra119.012239.
Full textBruegmann, Tobias, Khira Deecke, and Matthias Fladung. "Evaluating the Efficiency of gRNAs in CRISPR/Cas9 Mediated Genome Editing in Poplars." International Journal of Molecular Sciences 20, no. 15 (July 24, 2019): 3623. http://dx.doi.org/10.3390/ijms20153623.
Full textWardhani, Bantari W. K., Meidi U. Puteri, Yukihide Watanabe, Melva Louisa, Rianto Setiabudy, and Mitsuyasu Kato. "TMEPAI genome editing in triple negative breast cancer cells." Medical Journal of Indonesia 26, no. 1 (May 16, 2017): 14–8. http://dx.doi.org/10.13181/mji.v26i1.1871.
Full textKong, Qihui, Jie Li, Shoudong Wang, Xianzhong Feng, and Huixia Shou. "Combination of Hairy Root and Whole-Plant Transformation Protocols to Achieve Efficient CRISPR/Cas9 Genome Editing in Soybean." Plants 12, no. 5 (February 23, 2023): 1017. http://dx.doi.org/10.3390/plants12051017.
Full textJameel, Mohd Rizwan. "From design to validation of CRISPR/gRNA primers towards genome editing." Bioinformation 18, no. 5 (May 31, 2022): 471–77. http://dx.doi.org/10.6026/97320630018471.
Full textJung, Soo Bin, Chae young Lee, Kwang-Ho Lee, Kyu Heo, and Si Ho Choi. "A cleavage-based surrogate reporter for the evaluation of CRISPR–Cas9 cleavage efficiency." Nucleic Acids Research 49, no. 15 (June 4, 2021): e85-e85. http://dx.doi.org/10.1093/nar/gkab467.
Full textForeman, Hui-Chen Chang, Varvara Kirillov, Gabrielle Paniccia, Demetra Catalano, Trevor Andrunik, Swati Gupta, Laurie T. Krug, and Yue Zhang. "RNA-guided gene editing of the murine gammaherpesvirus 68 genome reduces infectious virus production." PLOS ONE 16, no. 6 (June 4, 2021): e0252313. http://dx.doi.org/10.1371/journal.pone.0252313.
Full textYoo, Byung-Chun, Narendra S. Yadav, Emil M. Orozco, and Hajime Sakai. "Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts." PeerJ 8 (January 6, 2020): e8362. http://dx.doi.org/10.7717/peerj.8362.
Full textDissertations / Theses on the topic "CRISPR, Cas9, genome editing, gRNA"
Roidos, Paris. "Genome editing with the CRISPR Cas9 system." Thesis, KTH, Skolan för bioteknologi (BIO), 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-163694.
Full textRan, Fei Ann. "CRISPR-Cas: Development and applications for mammalian genome editing." Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11610.
Full textHirosawa, Moe. "Cell-type-specific genome editing with a microRNA-responsive CRISPR-Cas9 switch." Kyoto University, 2019. http://hdl.handle.net/2433/242421.
Full textCastanon, velasco Oscar. "Targeting the transposable elements of the genome to enable large-scale genome editing and bio-containment technologies." Thesis, Université Paris-Saclay (ComUE), 2019. http://www.theses.fr/2019SACLX006.
Full textProgrammable and site-specific nucleases such as CRISPR-Cas9 have started a genome editing revolution, holding hopes to transform human health. Multiplexing or the ability to simultaneously introduce many distinct modifications in the genome will be required for basic and applied research. It will help to probe the physio-pathological functions of complex genetic circuits and to develop improved cell therapies or anti-viral treatments. By pushing the boundaries of genome engineering, we may reach a point where writing whole mammalian genomes will be possible. Such a feat may lead to the generation of virus-, cancer- or aging- free cell lines, universal donor cell therapies or may even open the way to de-extinction. In this doctoral research project, I outline the current state-of-the-art of multiplexed genome editing, the current limits and where such technologies could be headed in the future. We leveraged this knowledge as well as the abundant transposable elements present in our DNA to build an optimization pipeline and develop a new set of tools that enable large-scale genome editing. We achieved a high level of genome modifications up to three orders of magnitude greater than previously recorded, therefore paving the way to mammalian genome writing. In addition, through the observation of the cytotoxicity generated by multiple double-strand breaks within the genome, we developed a bio-safety switch that could potentially prevent the adverse effects of current and future cell therapies. Finally, I lay out the potential concerns and threats that such an advance in genome editing technology may be bringing and point out possible solutions to mitigate the risks
Valladares, Rodrigo, and Hanna Briheim. "Metoder och tillämpningar av CRISPR-Cas9 i cancerforskning. : Samt hur CRISPR-Cas9 kan implementeras i skolundervisningen." Thesis, Linköpings universitet, Institutionen för fysik, kemi och biologi, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-166140.
Full textCRISPR-Cas9 has recently emerged as an effective genome editing tool. The tool derives from an adaptive immune system in prokaryotes. The technology is used for modification of DNA in plants, animals and humans in a simple and inexpensive way. CRISPR-Cas9 has shown great potential in fighting different diseases like cancer which today is a global health issue. It is seen as a promising tool for cancer research when it comes to cancer therapy and drug development. Here we summarize current methods and applications of CRISPR-Cas9 for cancer research. Furthermore, we explore the possibilities of introducing and applying this kind of genetic engineering in biology teaching.
Framläggning, opponering och respondering skedde skriftligt till följd av covid19.
Toffessi, Tcheuyap Vanina. "Development of von Willebrand Factor Zebrafish Mutant Using CRISPR/Cas9 Mediated Genome Editing." Thesis, University of North Texas, 2017. https://digital.library.unt.edu/ark:/67531/metadc984227/.
Full textCanver, Matthew. "Elucidation of Mechanisms of Fetal Hemoglobin Regulation by CRISPR/Cas9 Mediated Genome Editing." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:33493407.
Full textMedical Sciences
Antoniani, Chiara. "A genome editing approach to induce fetal hemoglobin expression for the treatment of β-hemoglobinopathies." Thesis, Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCB077.
Full textΒ-hemoglobinopathies (β-thalassemias and sickle cell disease) are genetic anemias affecting thousands of newborns annually worldwide. β-thalassemias and sickle cell disease (SCD) are caused by mutations affecting the adult hemoglobin expression and are currently treated by red blood cell transfusion and iron chelation regiments. For patients affected by severe β-hemoglobinopathies, allogenic hematopoietic stem cell (HSCs) transplantation is the only definitive therapy. However, transplantation of autologous, genetically corrected HSCs represents an alternative therapy for patients lacking a suitable HSC donor. Naturally occurring large deletions encompassing β- and δ-globin genes in the β-globin gene cluster, defined as Hereditary Persistence of Fetal Hemoglobin (HPFH) traits, lead to increased fetal hemoglobin (HbF) expression ameliorating both thalassemic and SCD clinical phenotypes. In this study, we integrated transcription factor binding site analysis and HPFH genetic data to identify potential HbF silencers in the β-globin locus. Based on this analysis, we designed a CRISPR/Cas9 strategy disrupting: (i) a putative δγ-intergenic HbF silencer targeted by the HbF repressor BCL11A in adult erythroblasts; (ii) the shortest deletion associated with elevated HbF levels (“Corfu” deletion) in β-thalassemic patients, encompassing the putative δγ-intergenic HbF silencer; (iii) a 13.6-kb genomic region including the δ- and β-globin genes and the putative intergenic HbF silencer. Targeting the 13.6-kb region, but not the Corfu and the putative δγ-intergenic regions, caused a robust HbF re-activation and a concomitant reduction in β-globin expression in an adult erythroid cell line and in healthy donor hematopoietic stem/progenitor cells (HSPC)-derived erythroblasts. We provided a proof of principle of this potential therapeutic strategy: disruption of the 13.6-kb region in HSPCs from SCD donors favored the β-to-γ globin switching in a significant proportion of HSPC-derived erythroblasts, leading to the amelioration of the SCD cell phenotype. Finally, we dissected the mechanisms leading to HbF de-repression demonstrating changes in the chromatin conformation and epigenetic modifications within the β-globin locus upon deletion or inversion of the 13.6-kb region. Overall, this study contributes to the knowledge of the mechanisms underlying fetal to adult hemoglobin switching, and provides clues for a genome editing approach to the treatment of SCD and β-thalassemia
Lin, ChieYu. "Characterization and Optimization of the CRISPR/Cas System for Applications in Genome Engineering." Thesis, Harvard University, 2014. http://etds.lib.harvard.edu/hms/admin/view/61.
Full textHsu, Patrick David. "Development of the CRISPR nuclease Cas9 for high precision mammalian genome engineering." Thesis, Harvard University, 2014. http://nrs.harvard.edu/urn-3:HUL.InstRepos:13068392.
Full textBooks on the topic "CRISPR, Cas9, genome editing, gRNA"
María Vaschetto, Luis. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases. New York: CRC Press, 2021. http://dx.doi.org/10.1201/9781003088516.
Full textKozubek, James. Modern Prometheus: Editing the Human Genome with Crispr-Cas9. Cambridge University Press, 2016.
Find full textRavindhran, Ramalingam, Govindan Ganesan, and Vinoth A. Crop Genome Editing Using CRISPR/Cas9: Theory and Practice. Elsevier Science & Technology, 2019.
Find full textModern Prometheus: Editing the Human Genome with Crispr-Cas9. Cambridge University Press, 2018.
Find full textKozubek, Jim. Modern Prometheus: Editing the Human Genome with Crispr-Cas9. Cambridge University Press, 2018.
Find full textRavindhran, Ramalingam, Govindan Ganesan, and Vinoth A. Crop Genome Editing Using CRISPR/Cas9: Theory and Practice. Elsevier Science & Technology Books, 2019.
Find full textKozubek, James. Modern Prometheus: Editing the Human Genome with Crispr-Cas9. Cambridge University Press, 2019.
Find full textVaschetto, Luis María. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases. Taylor & Francis Group, 2021.
Find full textVaschetto, Luis M. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases. CRC Press LLC, 2022.
Find full textVaschetto, Luis M. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases. Taylor & Francis Group, 2022.
Find full textBook chapters on the topic "CRISPR, Cas9, genome editing, gRNA"
Hoof, Jakob B., Christina S. Nødvig, and Uffe H. Mortensen. "Genome Editing: CRISPR-Cas9." In Methods in Molecular Biology, 119–32. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7804-5_11.
Full textSeruggia, Davide, and Lluis Montoliu. "CRISPR/Cas9 Approaches to Investigate the Noncoding Genome." In Genome Editing, 31–43. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-34148-4_2.
Full textPatial, Meghna, Kiran Devi, and Rohit Joshi. "CRISPR/Cas9-Mediated Targeted Mutagenesis in Medicinal Plants." In Genome Editing, 55–70. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-08072-2_3.
Full textBansal, Monika, and Shabir Hussain Wani. "Virus-Mediated Delivery of CRISPR/CAS9 System in Plants." In Genome Editing, 197–203. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-08072-2_10.
Full textGurumurthy, Channabasavaiah B., Rolen M. Quadros, Masahiro Sato, Tomoji Mashimo, K. C. Kent Lloyd, and Masato Ohtsuka. "CRISPR/Cas9 and the Paradigm Shift in Mouse Genome Manipulation Technologies." In Genome Editing, 65–77. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-34148-4_4.
Full textKatam, Ramesh, Fatemeh Hasanvand, Vinson Teniyah, Jessi Noel, and Virginia Gottschalk. "Biosafety Issue Related to Genome Editing in Plants Using CRISPR-Cas9." In Genome Editing, 289–317. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-08072-2_16.
Full textSaeed, Fozia, Tariq Shah, Sherien Bukhat, Fazal Munsif, Ijaz Ahmad, Hamad Khan, and Aziz Khan. "Genome Engineering as a Tool for Enhancing Crop Traits: Lessons from CRISPR/Cas9." In Genome Editing, 3–25. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-031-08072-2_1.
Full textNadakuduti, Satya Swathi, Colby G. Starker, Daniel F. Voytas, C. Robin Buell, and David S. Douches. "Genome Editing in Potato with CRISPR/Cas9." In Methods in Molecular Biology, 183–201. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8991-1_14.
Full textLiu, Junqi, Samatha Gunapati, Nicole T. Mihelich, Adrian O. Stec, Jean-Michel Michno, and Robert M. Stupar. "Genome Editing in Soybean with CRISPR/Cas9." In Methods in Molecular Biology, 217–34. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8991-1_16.
Full textVelusamy, Thilaga, Anjali Gowripalan, and David C. Tscharke. "CRISPR/Cas9-Based Genome Editing of HSV." In Methods in Molecular Biology, 169–83. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9814-2_9.
Full textConference papers on the topic "CRISPR, Cas9, genome editing, gRNA"
Shukla, Jayanti, Bhairvi Pant, Neema Tufchi, Gracy Chand Paul, Kumud Pant, Manu Pant, and Somya Sihna. "CRISPR Cas9 genome editing approach for gRNA of the genes associated with Schizophrenia: A computational approach." In 2022 2nd International Conference on Innovative Sustainable Computational Technologies (CISCT). IEEE, 2022. http://dx.doi.org/10.1109/cisct55310.2022.10046633.
Full textStacey, Minviluz. "Utility of CRISPR/Cas in accelerating gene discovery in soybean." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/rzne1660.
Full textMETLEVA, Anastasia S., and Konstantin V. BESPOMESTNYKH. "Selection of Grna for Genomic Editing of the Bovine Leucosis Virus Susceptible Alleles of the 2 Exon of the Bola-DRB3 Gene by CRISPR/Cas9." In IV International Scientific and Practical Conference "Modern S&T Equipments and Problems in Agriculture". Sibac, 2020. http://dx.doi.org/10.32743/kuz.mepa.2020.148-157.
Full textTedesco, Donato, Paul Diehl, Mikhail Makhanov, Sylvain Baron, Dmitry Suchkov, and Alex Chenchik. "Abstract C161: CRISPR/Cas9 genome-wide gRNA library screening platform." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics; November 5-9, 2015; Boston, MA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1535-7163.targ-15-c161.
Full textTedesco, Donato, Paul Diehl, Mikhail Makhanov, Sylvain Baron, Dmitry Suchkov, Costa Frangou, and Alex Chenchik. "Abstract 4354: CRISPR/Cas9 genome-wide gRNA library for target identification." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-4354.
Full textRastogi, Khushboo. "Rice Biofortification through CRISPR/Cas9-Multiplex Genome Editing." In ASPB PLANT BIOLOGY 2020. USA: ASPB, 2020. http://dx.doi.org/10.46678/pb.20.1383191.
Full textShima, K., T. Suzuki, Y. Ma, C. Mayhew, A. Sallese, B. C. Carey, P. Arumugam, and B. C. Trapnell. "CRISPR/Cas9 Genome Editing Therapy for Hereditary Pulmonary Alveolar Proteinosis." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a4004.
Full textКершанская, О. И., Г. Л. Есенбаева, Д. С. Нелидова, З. Н. Садуллаева, and С. Н. Нелидов. "PERSPECTIVES OF BREEDING DEVELOPMENT IN BARLEY THROUGH CRISPR/CAS9 GENOME EDITING." In Материалы I Всероссийской научно-практической конференции с международным участием «Геномика и современные биотехнологии в размножении, селекции и сохранении растений». Crossref, 2020. http://dx.doi.org/10.47882/genbio.2020.48.47.015.
Full textZheng, Qi, Ling-Jie Kong, Huanyu Jin, Jinling Li, and Ruby Yanru Chen-Tsai. "Abstract 663: Factors affecting genome editing using CRISPR/Cas9 in mouse model." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-663.
Full text"CRISPR/Cas9 – mediated genome editing of bread wheat to modulate heading time." In Plant Genetics, Genomics, Bioinformatics, and Biotechnology. Novosibirsk ICG SB RAS 2021, 2021. http://dx.doi.org/10.18699/plantgen2021-135.
Full textReports on the topic "CRISPR, Cas9, genome editing, gRNA"
Paran, Ilan, and Allen Van Deynze. Regulation of pepper fruit color, chloroplasts development and their importance in fruit quality. United States Department of Agriculture, January 2014. http://dx.doi.org/10.32747/2014.7598173.bard.
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