Journal articles on the topic 'CRISPR/Cas9, flow cytometry, order of event'
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Lanjewar, S. N., and K. R. Bondioli. "205 Optimization of Transfection Efficiency for CRISPR/Cas9-Induced Genomic Editing in Porcine Fibroblast Cells." Reproduction, Fertility and Development 30, no. 1 (2018): 243. http://dx.doi.org/10.1071/rdv30n1ab205.
Full textKaur, Surinder, Stephen C. Alley, Matt Szapacs, Amanda Wilson, Eugene Ciccimaro, Dian Su, Neil Henderson, et al. "2021 White Paper on Recent Issues in Bioanalysis: Mass Spec of Proteins, Extracellular Vesicles, CRISPR, Chiral Assays, Oligos; Nanomedicines Bioanalysis; ICH M10 Section 7.1; Non-Liquid & Rare Matrices; Regulatory Inputs (Part 1A – Recommendations on Endogenous Compounds, Small Molecules, Complex Methods, Regulated Mass Spec of Large Molecules, Small Molecule, PoC & Part 1B - Regulatory Agencies' Inputs on Bioanalysis, Biomarkers, Immunogenicity, Gene & Cell Therapy and Vaccine)." Bioanalysis 14, no. 9 (May 2022): 505–80. http://dx.doi.org/10.4155/bio-2022-0078.
Full textCorat, Marcus A. F., Jean-Yves Metais, and Cynthia E. Dunbar. "Progress Towards Creation of a Rhesus Macaque Animal Model for PNH Disease Via Crispr/Cas9 Technology to Knock out the PIG-a Gene." Blood 124, no. 21 (December 6, 2014): 4389. http://dx.doi.org/10.1182/blood.v124.21.4389.4389.
Full textPabst, Gabriel, Johannes Foßelteder, Angelika Schlacher, Lisa Auinger, Daniel Martinez-Krams, Asiri Ediriwickrema, Karl Kashofer, et al. "Modeling the Development of SRSF2 Mutated Myeloid Malignancies By CRISPR/Cas9 Mediated Genome Engineering of Primary Human Hematopoietic Stem and Progenitor Cells." Blood 138, Supplement 1 (November 5, 2021): 2160. http://dx.doi.org/10.1182/blood-2021-149591.
Full textAdriaanse, Fabienne R. S., Sadie M. Sakurada, Shondra M. Pruett-Miller, Ronald W. Stam, Michel C. Zwaan, and Tanja A. Gruber. "Non-Coding HOX Fusions in Pediatric Non-Down Syndrome Acute Megakaryoblastic Leukemia." Blood 134, Supplement_1 (November 13, 2019): 533. http://dx.doi.org/10.1182/blood-2019-127014.
Full textIvy, Kathryn S., Candace H. Cote, and Paul Brent Ferrell. "IDH2 Mutations Induce Altered STAT Signaling and Cytokine Responses Which Are Restored By Enasidenib." Blood 132, Supplement 1 (November 29, 2018): 1468. http://dx.doi.org/10.1182/blood-2018-99-117783.
Full textHaase-Kohn, Cathleen, Markus Laube, Cornelius K. Donat, Birgit Belter, and Jens Pietzsch. "CRISPR/Cas9 Mediated Knockout of Cyclooxygenase-2 Gene Inhibits Invasiveness in A2058 Melanoma Cells." Cells 11, no. 4 (February 21, 2022): 749. http://dx.doi.org/10.3390/cells11040749.
Full textBeigl, Tobias B., Ine Kjosås, Emilie Seljeseth, Nina Glomnes, and Henriette Aksnes. "Efficient and crucial quality control of HAP1 cell ploidy status." Biology Open 9, no. 11 (November 12, 2020): bio057174. http://dx.doi.org/10.1242/bio.057174.
Full textMishima, Yuji, Jiantao Shi, Michele Moschetta, Yujia Shen, Salomon Manier, Aldo M. Roccaro, Francois Mercier, et al. "In Vivo Analysis of Clonal Evolution of Multiple Myeloma." Blood 128, no. 22 (December 2, 2016): 799. http://dx.doi.org/10.1182/blood.v128.22.799.799.
Full textFoßelteder, Johannes, Angelika Schlacher, Gabriel Pabst, Bettina Amtmann, Wolfgang Schöll, Karl Kashofer, Christine Beham-Schmid, et al. "Introduction and Genetic Correction of Calreticulin Mutations in Human Hematopoietic Stem and Progenitor Cells Sheds Light on MPN Pathogenesis." Blood 138, Supplement 1 (November 5, 2021): 2541. http://dx.doi.org/10.1182/blood-2021-147919.
Full textCastro, Jesus, Mariana Gaelzer, and Scott Welford. "Abstract 209: Elucidating the role of NMDA subunit NR2B in non-tumor and tumor-bearing mice." Cancer Research 82, no. 12_Supplement (June 15, 2022): 209. http://dx.doi.org/10.1158/1538-7445.am2022-209.
Full textDong, Mengmeng, Enfan Zhang, Haimeng Yan, Ruyi Xu, He Huang, Anyong Xie, and Zhen Cai. "Macrophages Promote DNA Repair of Double Strand Break in Multiple Myeloma Cells By Non-Homologous End Joining(NHEJ), Nevertheless Decrease Its Accuracy." Blood 134, Supplement_1 (November 13, 2019): 3087. http://dx.doi.org/10.1182/blood-2019-126753.
Full textWakabayashi, Aoi, Maryanne Kihiu, Malini Sharma, Mathieu Quesnel-Vallieres, Osheiza Abdulmalik, Scott A. Peslak, Cheryl A. Keller, et al. "Interrogating Post-Transcriptional Mechanisms of Fetal Hemoglobin Regulation." Blood 138, Supplement 1 (November 5, 2021): 3079. http://dx.doi.org/10.1182/blood-2021-151488.
Full textBerrien-Elliott, Melissa M., Wong Pamela, Carly Neal, Julia A. Wagner, Michelle Becker-Hapak, Tim Schappe, Matthew L. Cooper, Emily M. Mace, and Todd A. Fehniger. "Primary Human NK Cell Gene-Editing Reveals a Critical Role for NKG2A in Cytokine-Induced Memory-like NK Cell Responses." Blood 134, Supplement_1 (November 13, 2019): 3237. http://dx.doi.org/10.1182/blood-2019-129162.
Full textPilunov, Artem, Dmitrii Romaniuk, Savely Sheetikov, Alexandra Khmelevskaya, Anton Shmelev, Nadia Bykova, Alina Shomuradova, et al. "Development of T-Cell Therapy Targeting Hematopoietic Minor Histocompatibility Antigen HA-1." Blood 134, Supplement_1 (November 13, 2019): 5749. http://dx.doi.org/10.1182/blood-2019-130552.
Full textShen, Yandong, Kyle R. Crassini, Michael E. O'Dwyer, Michael Francis O'Neill, Richard Christopherson, Stephen P. Mulligan, and Oliver Giles Best. "The Dual PI3/PIM-Kinase Inhibitor, Ibl-202, Is Highly Synergistic with Venetoclax Against CLL Cells, and TP53-Knock-out Cells, and Under Conditions That Mimic the Tumor Microenvironment." Blood 132, Supplement 1 (November 29, 2018): 1870. http://dx.doi.org/10.1182/blood-2018-99-115574.
Full textGupta, Dipti, Yannis Hara, Samuel Lessard, Sarah Sturtevant, Yukio Nakamura, Sriram Krishnamoorthy, Melanie Demers, and Alexandra Hicks. "Catalytic Activity of Heme-Regulated eIF2 Alpha Kinase (HRI) Regulates Fetal Hemoglobin." Blood 136, Supplement 1 (November 5, 2020): 7. http://dx.doi.org/10.1182/blood-2020-139991.
Full textWenthe, Sophia, Kelsie Becklin, Brett Napiwocki, Emma Kozurek, Branden Moriarity, and Jong Hyuk Kim. "Abstract 198: Unveiling chromatin accessibility landscape and convergent oncogenic pathway in angiosarcoma models using induced pluripotent stem cells." Cancer Research 82, no. 12_Supplement (June 15, 2022): 198. http://dx.doi.org/10.1158/1538-7445.am2022-198.
Full textAruljothi, Charlesantony, Subin S. George, Patrick Somers, Justin Blum, Chelsea Thorsheim, Maxim Pimkin, and Vikram R. Paralkar. "Regulation of Ribosomal RNA Synthesis in Myeloid Progenitors By Cell Type Specific Transcription Factors." Blood 136, Supplement 1 (November 5, 2020): 11–12. http://dx.doi.org/10.1182/blood-2020-138776.
Full textMeyer, Tatjana, Nikolaus Jahn, Anna Dolnik, Peter Paschka, Verena I. Gaidzik, Daniela Weber, Lars Bullinger, Hartmut Döhner, Konstanze Döhner, and Jan Kroenke. "BRCA1/2 Containing Complex 3 (BRCC36) Is Recurrently Mutated in AML with t(8;21) and Associated with Increased Sensitivity to Chemotherapy through Impairment of the DNA Damage Repair Pathway." Blood 132, Supplement 1 (November 29, 2018): 1487. http://dx.doi.org/10.1182/blood-2018-99-114143.
Full textStopka, Tomas, Jarmila Vargova, Karina Vargova, Nina Dusilkova, Vojtech Kulvait, Vit Pospisil, Jiri Zavadil, Marek Trneny, and Pavel Klener. "Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) Is a New Biomarker for Mantle Cell Lymphoma: Expression, Localization, and Phosphorylation Study." Blood 128, no. 22 (December 2, 2016): 1767. http://dx.doi.org/10.1182/blood.v128.22.1767.1767.
Full textBarrio, Santiago, Larissa Haertle, Umair Munawar, Lucia Martin, Isabel Cuenca, Cornelia Vogt, Andoni Garitano-Trojaola, et al. "Clonal Competition Models to Understand Progression and Resistance in Myeloma." Blood 134, Supplement_1 (November 13, 2019): 1807. http://dx.doi.org/10.1182/blood-2019-130812.
Full textCouch, Tyler A., Zachary C. Murphy, Michael Getman, Ryo Kurita, Yukio Nakamura, and Laurie A. Steiner. "Human Erythroblasts with c-Kit Activating Mutations Have Reduced Cell Culture Costs and Remain Capable of Terminal Maturation and Enucleation." Blood 132, Supplement 1 (November 29, 2018): 2315. http://dx.doi.org/10.1182/blood-2018-99-117157.
Full textEl Hoss, Sara, Sylvie Cochet, Auria Godard, Hongxia Yan, Michaël Dussiot, Giacomo Frati, Bénédicte Boutonnat-Faucher, et al. "Fetal Hemoglobin Rescues Ineffective Erythropoiesis in Sickle Cell Disease." Blood 136, Supplement 1 (November 5, 2020): 14–15. http://dx.doi.org/10.1182/blood-2020-137477.
Full textHeatley, Susan L., Elyse C. Page, Laura N. Eadie, Barbara J. McClure, Jacqueline Rehn, David T. Yeung, Michael Philip Osborn, Tamas Revesz, Maria Kirby, and Deborah L. White. "Modeling Relapsed, Refractory Acute Lymphoblastic Leukemia from a Child with Neurofibromatosis." Blood 138, Supplement 1 (November 5, 2021): 1317. http://dx.doi.org/10.1182/blood-2021-150086.
Full textTakata, Katsuyoshi, Daisuke Ennishi, Ali Bashashati, Saeed Saberi, Elena Viganò, Shannon Healy, Julie S. Nielsen, et al. "Somatic PRAME Deletions Are Associated with Decreased Immunogenicity, Apoptosis Resistance and Poor Outcomes in Diffuse Large B-Cell Lymphoma." Blood 132, Supplement 1 (November 29, 2018): 667. http://dx.doi.org/10.1182/blood-2018-99-113516.
Full textGrzegorski, Steven, Divyani Paul, James H. Morrissey, and Jordan A. Shavit. "Genetic Duplication of Tissue Factor Leads to Partial Specialization of Function in Zebrafish." Blood 134, Supplement_1 (November 13, 2019): 486. http://dx.doi.org/10.1182/blood-2019-131980.
Full textRimando, Joseph, Michael P. Rettig, Matt Christopher, Julie K. Ritchey, Miriam Y. Kim, John Muth, Jan Davidson, and John F. DiPersio. "Flotetuzumab and Other Cellular Immunotherapies Upregulate MHC Class II Expression on Acute Myeloid Leukemia Cells in Vitro and In Vivo." Blood 136, Supplement 1 (November 5, 2020): 22–23. http://dx.doi.org/10.1182/blood-2020-133891.
Full textHersey, Sarah, Steve Keller, Joel Mathews, Lindsay King, Abbas Bandukwala, Flora Berisha, Mary Birchler, et al. "2021 White Paper on Recent Issues in Bioanalysis: ISR for Biomarkers, Liquid Biopsies, Spectral Cytometry, Inhalation/Oral & Multispecific Biotherapeutics, Accuracy/LLOQ for Flow Cytometry (Part 2 – Recommendations on Biomarkers/CDx Assays Development & Validation, Cytometry Validation & Innovation, Biotherapeutics PK LBA Regulated Bioanalysis, Critical Reagents & Positive Controls Generation)." Bioanalysis, May 17, 2022. http://dx.doi.org/10.4155/bio-2022-0080.
Full textLoo, Lina, Shannon Harris, Mark Milton, Meena, Wibke Lembke, Flora Berisha, Sylvie Bertholet, et al. "2021 White Paper on Recent Issues in Bioanalysis: TAb/NAb, Viral Vector CDx, Shedding Assays; CRISPR/Cas9 & CAR-T Immunogenicity; PCR & Vaccine Assay Performance; ADA Assay Comparability & Cut Point Appropriateness (Part 3 – Recommendations on Gene Therapy, Cell Therapy, Vaccine Assays; Immunogenicity of Biotherapeutics and Novel Modalities; Integrated Summary of Immunogenicity Harmonization)." Bioanalysis, May 17, 2022. http://dx.doi.org/10.4155/bio-2022-0081.
Full textZoppo, Marina, Nicole Okoniewski, Stanislav Pantelyushin, Johannes vom Berg, and Kristin Schirmer. "A ribonucleoprotein transfection strategy for CRISPR/Cas9‐mediated gene editing and single cell cloning in rainbow trout cells." Cell & Bioscience 11, no. 1 (June 3, 2021). http://dx.doi.org/10.1186/s13578-021-00618-0.
Full textKamali, Elahe, Fatemeh Rahbarizadeh, Zohreh Hojati, and Morten Frödin. "CRISPR/Cas9-mediated knockout of clinically relevant alloantigenes in human primary T cells." BMC Biotechnology 21, no. 1 (January 29, 2021). http://dx.doi.org/10.1186/s12896-020-00665-4.
Full textPetković, Igor, Johannes Bischof, Thomas Kocher, Oliver Patrick March, Bernadette Liemberger, Stefan Hainzl, Dirk Strunk, et al. "COL17A1 editing via homology-directed repair in junctional epidermolysis bullosa." Frontiers in Medicine 9 (August 25, 2022). http://dx.doi.org/10.3389/fmed.2022.976604.
Full textChirikian, Orlando, William R. Goodyer, Elda Dzilic, Vahid Serpooshan, Jan W. Buikema, Wesley McKeithan, HaoDi Wu, et al. "CRISPR/Cas9-based targeting of fluorescent reporters to human iPSCs to isolate atrial and ventricular-specific cardiomyocytes." Scientific Reports 11, no. 1 (February 4, 2021). http://dx.doi.org/10.1038/s41598-021-81860-x.
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