Academic literature on the topic 'Creatinine Colorimetric Assay Kit'
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Journal articles on the topic "Creatinine Colorimetric Assay Kit"
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Elgamouz, Abdelaziz, Chahlaa Nassab, Alaa Bihi, Somaya A. I. Mohamad, Aisha H. S. A. Almusafri, Salman S. Alharthi, Sarah A. E. Abdulla, and Shashikant P. Patole. "Encapsulation Capacity of β-Cyclodextrin Stabilized Silver Nanoparticles towards Creatinine Enhances the Colorimetric Sensing of Hydrogen Peroxide in Urine." Nanomaterials 11, no. 8 (July 24, 2021): 1897. http://dx.doi.org/10.3390/nano11081897.
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The β-cyclodextrin shell of synthesized silver nanoparticles (βCD-AgNPs) are found to enhance the detection of hydrogen peroxide in urine when compared to the Horse Radish Peroxidase assay kit. Nanoparticles are confirmed by the UV-Vis absorbance of their localized surface plasmonic resonance (LSPR) at 384 nm. The mean size of the βCD-AgNPs is 53 nm/diameter; XRD analysis shows a face-centered cubic structure. The crystalline structure of type 4H hexagonal nature of the AgNPs with 2.4 nm β-CD coating onto is confirmed using aberration corrected high-resolution transmission electron microscopy (HRTEM). A silver atomic lattice at 2.50 Å and 2.41 Å corresponding to (100) and (101) Miller indices is confirmed using the HRTEM. The scope of βCD-AgNPs to detect hydrogen peroxide (H2O2) in aqueous media and human urine is investigated. The test is optimized by examining the effect of volumes of nanoparticles, the pH of the medium, and the kinetic and temperature effect on H2O2 detection. The βCD-AgNPs test is used as a refined protocol, which demonstrated improved sensitivity towards H2O2 in urine compared to the values obtained by the Horse Radish Assay kit. Direct assessment of H2O2 by the βCD-AgNPs test presented always with a linear response in the nM, μM, and mM ranges with a limit of detection of 1.47 nM and a quantitation limit of 3.76 nM. While a linear response obtained from 1.3 to 37.3 nmoles of H2O2/mole creatinine with a slope of 0.0075 and regression coefficient of 0.9955 when the βCD-AgNPs is used as refined test of creatinine. Values ranging from 34.62 ± 0.23 nmoles of H2O2/mole of creatinine and 54.61 ± 1.04 nmoles of H2O2/mole of creatinine when the matrix is not diluted and between 32.16 ± 0.42 nmoles of H2O2/mole of creatinine and 49.66 ± 0.80 nmoles of H2O2/mole of creatinine when the matrix is twice diluted are found in freshly voided urine of seven apparent healthy men aged between 20 and 40 years old.
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Pócsi, István, László Csáthy, V. Anna Oláh, and Robert G. Price. "Assay of N-Acetyl-β-D-Glucosaminidase in Urine from Neonates: Comparison of Two New Colorimetric Methods Using MNP-GlcNAc and VRA-GlcNAc as Substrates." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 29, no. 3 (May 1992): 292–95. http://dx.doi.org/10.1177/000456329202900307.
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The NAG activity present in urine from newborn babies was assayed using two colorimetric procedures with either MNP-GlcNAc or VRA-GlcNAc as substrate and compared with data obtained with the well established PNP-GlcNAc procedure. Both new assays were easy to perform and reproducible. The MNP-GlcNAc method has the advantage that it is now available as a kit; however, the VRA-GlcNAc procedure is more sensitive. NAG activity, creatinine concentration and NAG-index values were determined in normal neonates and within-run imprecision calculated. Excellent correlations were found between MNP-GlcNAc-ase and VRA-GlcNAc-ase indices ( r = 0·984) and between PNP-GlcNAc-ase and VRA-GlcNAc-ase indices ( r = 0.952). When low molecular weight urinary components were removed by gel filtration no significant change in VRA-GlcNAc-ase activity was observed.
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Houcher, Zahira, Bakhouche Houcher, Abderrezak Touabti, Samia Begag, Yonca Egin, and Nejat Akar. "Nutritional Factors, Homocysteine and C677T Polymorphism of the Methylenetetrahydrofolate Reductase Gene in Algerian Subjects with Cardiovascular Disease." Pteridines 23, no. 1 (February 2012): 14–21. http://dx.doi.org/10.1515/pteridines.2012.23.1.14.
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Abstract The C677T variant of methylenetetrahydrofolate reductase (MTHFR), a key enzyme in the remethylation of homocysteine (HCY) to methionine, is a frequent genetic cause of moderate hyperhomocysteinemia (HHCY) among individuals with cardiovascular disease (CVD), and particularly when combined with other factors such as hyperlipidaemia. However, in Algeria the influence of nutrient-gene interactions is not known. The aim of the present study was to explore the influence of age and gender, together with folate status, on the association between the C677T MTHFR polymorphism and plasma total HCY (tHCY) concentrations. This research was carried out as a prospective study on 98 patients hospitalized in the Cardiology Section, University of Sétif, Algeria. Mean age of participants was 57 y (range 20-96 y).The genetic analysis of the MTHFR C677T polymorphism was performed by real-time polymerase chain reaction (PCR) performed on Light Cycler in borosilicate capillaries with MTHFR 677CT polymorphism detection kit. The concentrations of tHCY, folic acid vitamin B12 levels were determined using a competitive immunoassay on the IMMULITE 1000 Analyzers. Plasma total cholesterol, triglycerides, glucose, creatinine and urea concentrations were measured by colorimetric methods. Assays were conducted according to the manufacturers' instructions. Plasma tHCY was significantly higher in the patients with CVD, and HHCY was associated with the presence of mildly elevated serum urea and creatinine (p <0.05). MTHFR gene mutation does not seem to be associated with elevation of plasma tHCY in the studied patients and this lack of correlation could be influenced by the higher folate concentrations in our study. CVD patients with 677CT/TT genotypes had a higher concentration of total cholesterol than those with 677CC genotype (p <0.05). Although, the presence of 677T variant together with hypofolatemia (<15.4 ng/ml) had a more detrimental effect on the level of total cholesterol (p <0.05). Folatemia and vitamin B12 were much higher in 677CC genotype compared to 677CT/TT genotype in CVD subjects without hyperlipidemia (p <0.05). However in patients with hyperlipidemia these values became lower also with 677CC genotype. In conclusion, hyperlipidemia affects the levels of plasma folate and vitamin B12 concentrations independent of mutated MTHFR genotype. The effect of 677T variant on total cholesterol, folate and vitamin B12 concentrations may relate to possible adverse effects of elevated tHCY on lipid profiles and on plasma folate and vitamin B12.
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He, Yi, Xianhui Zhang, and Haili Yu. "Gold nanoparticles-based colorimetric and visual creatinine assay." Microchimica Acta 182, no. 11-12 (June 23, 2015): 2037–43. http://dx.doi.org/10.1007/s00604-015-1546-0.
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Larpent, Liliane, and Christian Verger. "The Need for Using An Enzymatic Colorimetric Assay in Creatinine Determination of Peritoneal Dialysis Solutions." Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis 10, no. 1 (January 1990): 89–92. http://dx.doi.org/10.1177/089686089001000122.
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The fate of the peritoneal membrane on continuous ambulatory peritoneal dialysis (CAPD) is usually evaluated through the modification of its permeability to various solutes as glucose, creatinine, and urea. Therefore, the accuracy of the methods used for measurements of creatinine is of great importance. A particular problem does exist for creatinine determination as it may be influenced by the presence of glucose. We studied a new enzymatic colorimetric method for creatinine determination in peritoneal dialysis solutions which contain high dextrose concentrations. Creatinine was measured in plasma, urine, and dialysate from 18 patients on CAPD and in pure dextrose solutions, with an enzymatic test (Boehringer Mannheim) and with Jaffe's reaction on two different analyzers: Astra (Beckman) and Eris (Merck). Creatinine results were similar with both assays (Jaffe's reaction and enzymatic test) when measured in blood and urine. However the Jaffe's reaction gave higher creatinine results than the enzymatic test (p < 0.001), when assays were performed in peritoneal dialysis solutions and in pure glucose solutions. In addition, it appeared that other components of dialysis solutions, mainly calcium chloride, influenced unpredictably the results of creatinine with the Jaffe's reaction. We conclude that specific enzymatic test is a more accurate and reliable method to evaluate creatinine kinetics through the peritoneal membrane when determined in CAPD solutions.
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Krausz, Alyse D., Rajan Dewar, and Mark A. Burns. "Accuracy Evaluation of a Tetrabromophenolphthalein Ethyl Ester Colorimetric Assay for Urinary Albumin." Journal of Applied Laboratory Medicine 4, no. 2 (September 1, 2019): 201–13. http://dx.doi.org/10.1373/jalm.2019.030031.
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Abstract Background The tetrabromophenolphthalein ethyl ester (TBPE) assay has been used to quantify urinary albumin in point-of-care devices. We assessed the accuracy of this TBPE assay for urinary albumin through comparison with an established immunoturbidimetric method (ADVIA 1800 Chemistry System, Siemens). Methods We developed a TBPE assay protocol to quantify albumin in the range associated with microalbuminuria (0–200 mg/L). The Jaffe reaction and a 3-dimensional (3D) surface were used to compensate for creatinine interference. Spiked simulated urine samples and patient samples were used to compare the TBPE assay with the immunoturbidimetric method. Multiple linear regression was used to analyze factors that could account for discrepancies between the 2 methods. Results We found that creatinine interfered with the TBPE assay. To compensate, a 3D surface was successfully used to quantify albumin in spiked deionized water and simulated urine samples. In spiked simulated urine samples, the immunoturbidimetric method underestimated the albumin concentration by 2 to 45 mg/L, and the TBPE assay overestimated it by 9 to 82 mg/L. In patient samples, the albumin concentrations measured with the TBPE assay and the immunoturbidimetric method differed by an average of 184 mg/L. Conclusions The TBPE assay is a function of the creatinine concentration, and a 3D surface can be used to provide accurate albumin concentrations for standard samples. The corrected TBPE method and the immunoturbidimetric method deviated from known concentrations of spiked samples. Further investigation and comparisons with a third albumin measurement method, such as LC-MS/MS, are necessary before conclusions on the accuracy of the TBPE assay can be made.
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Magnotti, R. A., G. W. Stephens, R. K. Rogers, and A. J. Pesce. "Microplate measurement of urinary albumin and creatinine." Clinical Chemistry 35, no. 7 (July 1, 1989): 1371–75. http://dx.doi.org/10.1093/clinchem/35.7.1371.
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Abstract We describe microplate methods for measurement of human urinary albumin (HUA) by competitive enzyme-linked immunosorbant assay (ELISA) and creatinine with a modified commercial enzymatic kit. Incorporation of substrate mixing into the competitive ELISA changes the dynamic absorbance-concentration response, greatly simplifying calculations and improving sensitivity and accuracy. Measurement of creatinine in urine and plasma samples with a commercially available enzymatic kit modified for analysis by use of an inexpensive microplate reader produced values comparable in precision and accuracy to those obtained by an automated kinetic Jaffé method.
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Paz, Matt Andrew, and Jesse Seegmiller. "Interference on the Abbott i-STAT creatinine assay caused by hydroxyurea." American Journal of Clinical Pathology 158, Supplement_1 (November 1, 2022): S19—S20. http://dx.doi.org/10.1093/ajcp/aqac126.034.
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Abstract Serum creatinine is an important biomarker used for estimating glomerular filtration rate (eGFR). The present study was designed after a patient at our institution had a significantly elevated creatinine result taken from an i-STAT point of care test that utilizes electrochemical detection. The patient’s creatinine from the i-STAT was 3.8 mg/dL and was not consistent with their clinical history. The patient’s previous creatinine taken 2 days prior was 1.39 mg/dL and 1.27 mg/dL seven days prior. Both previous readings were measured with the Siemens Dimension Vista 1500 using a colorimetric enzymatic creatinine method. A subsequent creatinine measured on the Siemens Dimension Vista 1500 was 1.30 mg/dL. Upon chart review, the clinical team noted that the patient is taking 500 mg of hydroxyurea daily for treatment of a myeloproliferative disorder. Review of the i-STAT package insert revealed hydroxyurea as a known interfering substance and states for every 100 µmol/L hydroxyurea in the specimen, creatinine will be increased by approximately 1.85 mg/dL. Review of literature described the positive interference caused by hydroxyurea for the i-STAT creatinine and glucose assays but did not explain the mechanism of this positive interference. In order to characterize the mechanism of hydroxyurea interference, we investigated the situation further. A pool of plasma was spiked with hydroxyurea. The spiked pool was serial diluted (x2, x4, x8, x16, x32, x64, x128) to observe the creatinine results in the presence of and absence of hydroxyurea. The dilution series was analyzed using the Vista enzymatic creatinine and the Abbott i-STAT enzymatic creatinine methods. The i-STAT creatinine results show increasing creatinine concentration from interference as the concentration of hydroxyurea increased. The dilution series suggests increasing positive interference with hydroxyurea. Positive creatinine interference with hydroxyurea was not observed when analyzed on the Vista platform. Hydroxyurea is a widely used treatment for myeloproliferative disorders and also for managing sickle cell disease. Hydroxyurea is known to cause positive interference in the i-STAT enzymatic creatine method that uses electrochemical detection, with the degree of interference correlating with the concentration of hydroxyurea. The Vista colorimetric enzymatic creatinine method was not observed to have hydroxyurea interference. While both methods employ enzymatic reagent systems the final detection approach is important. Patients undergoing hydroxyurea treatment should avoid i-STAT measurement systems for creatinine.
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Yuen, Peter S. T., Stephen R. Dunn, Takehiko Miyaji, Hideo Yasuda, Kumar Sharma, and Robert A. Star. "A simplified method for HPLC determination of creatinine in mouse serum." American Journal of Physiology-Renal Physiology 286, no. 6 (June 2004): F1116—F1119. http://dx.doi.org/10.1152/ajprenal.00366.2003.
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Mouse models are frequently used to study renal function. However, mouse serum contains chromagens that interfere with standard picric acid-based assays for serum creatinine. Several alternative methods exist for serum creatinine measurements, including assay by high-performance liquid chromatography (HPLC), but only one has been adapted to mouse serum. Creatinine was measured in serum by acetonitrile deproteinization, followed by isocratic, cation exchange HPLC. The HPLC method was compared with a standard alkaline picrate colorimetric assay, using serum from animals with low-to-moderate renal injury. Acidification of acetonitrile with HCl in the deproteinization step produced variable results, including an extra peak that interfered with integration of the creatinine peak or loss of the creatinine peak. Deproteinizing with acetonitrile alone resulted in a more reliable measurement of serum creatinine, which was validated by a series of known additions of creatinine standard. The HPLC assay was reproducible with coefficients of variation from 1.6 to 5.1%. The picric acid assay overestimated serum creatinine, when directly compared with the HPLC assay. The extent of overestimation, up to sixfold, was greatest at normal (0.1 to 0.2 mg/dl) to moderately elevated (0.5 mg/dl) serum creatinine levels. Mouse serum contains substances that interfere with standard picric acid assays for creatinine. Our new HPLC assay can accurately detect creatinine from 5 μl of mouse serum. These results support the widespread adoption of HPLC to accurately measure serum creatinine in mouse models of renal injury.
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Gao, Jing-Jhong, Ching-Wei Chiu, Kuo-Hsing Wen, and Cheng-Sheng Huang. "A Compact Detection Platform Based on Gradient Guided-Mode Resonance for Colorimetric and Fluorescence Liquid Assay Detection." Sensors 21, no. 8 (April 15, 2021): 2797. http://dx.doi.org/10.3390/s21082797.
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This paper presents a compact spectral detection system for common fluorescent and colorimetric assays. This system includes a gradient grating period guided-mode resonance (GGP-GMR) filter and charge-coupled device. In its current form, the GGP-GMR filter, which has a size of less than 2.5 mm, can achieve a spectral detection range of 500–700 nm. Through the direct measurement of the fluorescence emission, the proposed system was demonstrated to detect both the peak wavelength and its corresponding intensity. One fluorescent assay (albumin) and two colorimetric assays (albumin and creatinine) were performed to demonstrate the practical application of the proposed system for quantifying common liquid assays. The results of our system exhibited suitable agreement with those of a commercial spectrometer in terms of the assay sensitivity and limit of detection (LOD). With the proposed system, the fluorescent albumin, colorimetric albumin, and colorimetric creatinine assays achieved LODs of 40.99 and 398 and 25.49 mg/L, respectively. For a wide selection of biomolecules in point-of-care applications, the spectral detection range achieved by the GGP-GMR filter can be further extended and the simple and compact optical path configuration can be integrated with a lab-on-a-chip system.
Conference papers on the topic "Creatinine Colorimetric Assay Kit"
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Tanaka, T., T. Itoh, T. Tsujinaka, M. Sakon, J. Kambayashi, and T. Mori. "AN EXPERIMENTAL MODEL TO STUDY INTERRELATIONSHIP BETWEEN HYPER-COAGULABLE STATE AND ORGAN FAILURE IN SEPSIS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644245.
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Disseminated intravascular coagulation (DIC) and multiple organ failure (MOF) are critical consequences of septic patients but the pathogenesis has not been fully elucidated yet, because of the complexities of clinical cases. Thereby, attempts were made to establish animal model with DIC & MOF without injecting endotoxin, bacteria or procoagulants into circulation, which may result in misleading conclusions. Twenty four male Japan albino rabbits were divided into the following 4 groups; [A]-ligation of bile duct and instillation of fecal suspension ( appr. 2 X 107 gram negative rods)into the proximal bile duct. [B]-ligation of bile duct and instillation of sterile saline. [C]-laparotomy alone. [D]-[A] receiving intravenous heparin (50u/kg/h). Citrated blood from femoral vein was collected before and 3,6,9 hours after the surgical procedure. After the last blood collectioh, animals were sacrificed and lungs, liver and kidneys were harvested for histological evaluation. During 9h observation period,.the rabbits were kept anesthetized with pentobarbiturate and were hydrated with 4ml/kg/h saline. All animals survived during the period. The following findings were obtained in [A]. Platelet count was gradually decreased (p<0.05) and significant leukopenia was noted at 9h (p<0.01). APTT and PT were significantly prolonged after 3h (p㌼0.01) after 6h. Plasma creatinine and bilirubin were significantly increased than those of [B] (p<0.01). The amount of endotoxin in circulating blood, determined by colorimetric assay, was significantly elevated up to 200pg/ml (p<0.01) . Histological examination revealed glomerular thrombi and focal necrosis of the liver. In [B], bilirubin was slightly elevated and PT was prolonged but much abnormal values were noted in [A]. Endotoxemia was not detected at all in [B]. In [D], the value of PT and fibrinogen was comparable to that of [B] and also the value of bilirubin and creatinine was close to that of [B]. Thus, we established sepsis model with reproducible hypercoagulable state and organ failure. The prevention of organ failure by heparin administration indicates that hypercoagulable state is common etiologic factor in occurrence of DIC and MOF in sepsis