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Academic literature on the topic 'CPF1'

Author: Grafiati

Published: 4 June 2021

Last updated: 3 February 2022

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Journal articles on the topic "CPF1"

1

Kent, N. A., J. S. Tsang, D. J. Crowther, and J. Mellor. "Chromatin structure modulation in Saccharomyces cerevisiae by centromere and promoter factor 1." Molecular and Cellular Biology 14, no. 8 (August 1994): 5229–41. http://dx.doi.org/10.1128/mcb.14.8.5229.

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CPF1 is an abundant basic-helix-loop-helix-ZIP protein that binds to the CDEI motif in Saccharomyces cerevisiae centromeres and in the promoters of numerous genes, including those encoding enzymes of the methionine biosynthetic pathway. Strains lacking CPF1 are methionine auxotrophs, and it has been proposed that CPF1 might positively influence transcription at the MET25 and MET16 genes by modulating promoter chromatin structure. We test this hypothesis and show that the regions surrounding the CDEI motifs in the MET25 and MET16 promoters are maintained in a nucleosome-free state and that this requires the entire CPF1 protein. However, the chromatin structure around the CDEI motifs does not change on derepression of transcription and does not correlate with the methionine phenotype of the cell. An intact CDEI motif but not CPF1 is required for transcriptional activation from a region of the MET25 upstream activation sequence. Our results suggest that CPF1 functions to modulate chromatin structure around the CDEI motif but that these changes at the MET25 and MET16 promoters do not explain how CPF1 functions to maintain methionine-independent growth. The presence of CPF1-dependent chromatin structures at these promoters leads to a weak repression of transcription.

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Gong, Chongzhi, Shengchan Huang, Rentao Song, and Weiwei Qi. "Comparative Study between the CRISPR/Cpf1 (Cas12a) and CRISPR/Cas9 Systems for Multiplex Gene Editing in Maize." Agriculture 11, no. 5 (May 10, 2021): 429. http://dx.doi.org/10.3390/agriculture11050429.

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Although the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been proved to be an efficient multiplex gene editing system in maize, it was still unclear how CRISPR/Cpf1 (Cas12a) system would perform for multiplex gene editing in maize. To this end, this study compared the CRISPR/Cpf1 system and CRISPR/Cas9 system for multiplex gene editing in maize. The bZIP transcription factor Opaque2 (O2) was used as the target gene in both systems. We found that in the T0 and T1 generations, the CRISPR/Cpf1 system showed lower editing efficiency than the CRISPR/Cas9 system. However, in the T2 generation, the CRISPR/Cpf1 system generated more types of new mutations. While the CRISPR/Cas9 system tended to edit within the on-target range, the CRISPR/Cpf1 system preferred to edit in between the targets. We also found that in the CRISPR/Cpf1 system, the editing efficiency positively correlated with the expression level of Cpf1. In conclusion, the CRISPR/Cpf1 system offers alternative choices for target-site selection for multiplex gene editing and has acceptable editing efficiency in maize and is a valuable alternative choice for gene editing in crops.

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3

Kent, N. A., J. S. Tsang, D. J. Crowther, and J. Mellor. "Chromatin structure modulation in Saccharomyces cerevisiae by centromere and promoter factor 1." Molecular and Cellular Biology 14, no. 8 (August 1994): 5229–41. http://dx.doi.org/10.1128/mcb.14.8.5229-5241.1994.

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CPF1 is an abundant basic-helix-loop-helix-ZIP protein that binds to the CDEI motif in Saccharomyces cerevisiae centromeres and in the promoters of numerous genes, including those encoding enzymes of the methionine biosynthetic pathway. Strains lacking CPF1 are methionine auxotrophs, and it has been proposed that CPF1 might positively influence transcription at the MET25 and MET16 genes by modulating promoter chromatin structure. We test this hypothesis and show that the regions surrounding the CDEI motifs in the MET25 and MET16 promoters are maintained in a nucleosome-free state and that this requires the entire CPF1 protein. However, the chromatin structure around the CDEI motifs does not change on derepression of transcription and does not correlate with the methionine phenotype of the cell. An intact CDEI motif but not CPF1 is required for transcriptional activation from a region of the MET25 upstream activation sequence. Our results suggest that CPF1 functions to modulate chromatin structure around the CDEI motif but that these changes at the MET25 and MET16 promoters do not explain how CPF1 functions to maintain methionine-independent growth. The presence of CPF1-dependent chromatin structures at these promoters leads to a weak repression of transcription.

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4

Singh, Digvijay, John Mallon, Anustup Poddar, Yanbo Wang, Ramreddy Tippana, Olivia Yang, Scott Bailey, and Taekjip Ha. "Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)." Proceedings of the National Academy of Sciences 115, no. 21 (May 7, 2018): 5444–49. http://dx.doi.org/10.1073/pnas.1718686115.

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CRISPR-Cas9, which imparts adaptive immunity against foreign genomic invaders in certain prokaryotes, has been repurposed for genome-engineering applications. More recently, another RNA-guided CRISPR endonuclease called Cpf1 (also known as Cas12a) was identified and is also being repurposed. Little is known about the kinetics and mechanism of Cpf1 DNA interaction and how sequence mismatches between the DNA target and guide-RNA influence this interaction. We used single-molecule fluorescence analysis and biochemical assays to characterize DNA interrogation, cleavage, and product release by three Cpf1 orthologs. Our Cpf1 data are consistent with the DNA interrogation mechanism proposed for Cas9. They both bind any DNA in search of protospacer-adjacent motif (PAM) sequences, verify the target sequence directionally from the PAM-proximal end, and rapidly reject any targets that lack a PAM or that are poorly matched with the guide-RNA. Unlike Cas9, which requires 9 bp for stable binding and ∼16 bp for cleavage, Cpf1 requires an ∼17-bp sequence match for both stable binding and cleavage. Unlike Cas9, which does not release the DNA cleavage products, Cpf1 rapidly releases the PAM-distal cleavage product, but not the PAM-proximal product. Solution pH, reducing conditions, and 5′ guanine in guide-RNA differentially affected different Cpf1 orthologs. Our findings have important implications on Cpf1-based genome engineering and manipulation applications.

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5

Zhu, Houxiang, and Chun Liang. "CRISPR-DT: designing gRNAs for the CRISPR-Cpf1 system with improved target efficiency and specificity." Bioinformatics 35, no. 16 (January 7, 2019): 2783–89. http://dx.doi.org/10.1093/bioinformatics/bty1061.

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Abstract Motivation The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cpf1 system has been successfully applied in genome editing. However, target efficiency of the CRISPR-Cpf1 system varies among different guide RNA (gRNA) sequences. Results In this study, we reanalyzed the published CRISPR-Cpf1 gRNAs data and found many sequence and structural features related to their target efficiency. With the aid of Random Forest in feature selection, a support vector machine model was created to predict target efficiency for any given gRNAs. We have developed the first CRISPR-Cpf1 web service application, CRISPR-DT (CRISPR DNA Targeting), to help users design optimal gRNAs for the CRISPR-Cpf1 system by considering both target efficiency and specificity. CRISPR-DT will empower researchers in genome editing. Availability and implementation CRISPR-DT, mainly implemented in Perl, PHP and JavaScript, is freely available at http://bioinfolab.miamioh.edu/CRISPR-DT. Supplementary information Supplementary data are available at Bioinformatics online.

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6

de Winde, J. H., and L. A. Grivell. "Global regulation of mitochondrial biogenesis in Saccharomyces cerevisiae: ABF1 and CPF1 play opposite roles in regulating expression of the QCR8 gene, which encodes subunit VIII of the mitochondrial ubiquinol-cytochrome c oxidoreductase." Molecular and Cellular Biology 12, no. 6 (June 1992): 2872–83. http://dx.doi.org/10.1128/mcb.12.6.2872.

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The multifunctional DNA-binding proteins ABF1 and CPF1 bind in a mutually exclusive manner to the promoter region of the QCR8 gene, which encodes 11-kDa subunit VIII of the Saccharomyces cerevisiae mitochondrial ubiquinol-cytochrome c oxidoreductase (QCR). We investigated the roles that the two factors play in transcriptional regulation of this gene. To this end, the overlapping binding sites for ABF1 and CPF1 were mutated and placed in the chromosomal context of the QCR8 promoter. The effects on transcription of the QCR8 gene were analyzed both under steady-state conditions and during nutritional shifts. We found that ABF1 is required for repressed and derepressed transcription levels and for efficient induction of transcription upon escape from catabolite repression, independently of DNA replication. CPF1 acts as a negative regulator, modulating the overall induction response. Alleviation of repression through CPF1 requires passage through the S phase. Implications of these findings for the roles played by ABF1 and CPF1 in global regulation of mitochondrial biogenesis are discussed.

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de Winde, J. H., and L. A. Grivell. "Global regulation of mitochondrial biogenesis in Saccharomyces cerevisiae: ABF1 and CPF1 play opposite roles in regulating expression of the QCR8 gene, which encodes subunit VIII of the mitochondrial ubiquinol-cytochrome c oxidoreductase." Molecular and Cellular Biology 12, no. 6 (June 1992): 2872–83. http://dx.doi.org/10.1128/mcb.12.6.2872-2883.1992.

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The multifunctional DNA-binding proteins ABF1 and CPF1 bind in a mutually exclusive manner to the promoter region of the QCR8 gene, which encodes 11-kDa subunit VIII of the Saccharomyces cerevisiae mitochondrial ubiquinol-cytochrome c oxidoreductase (QCR). We investigated the roles that the two factors play in transcriptional regulation of this gene. To this end, the overlapping binding sites for ABF1 and CPF1 were mutated and placed in the chromosomal context of the QCR8 promoter. The effects on transcription of the QCR8 gene were analyzed both under steady-state conditions and during nutritional shifts. We found that ABF1 is required for repressed and derepressed transcription levels and for efficient induction of transcription upon escape from catabolite repression, independently of DNA replication. CPF1 acts as a negative regulator, modulating the overall induction response. Alleviation of repression through CPF1 requires passage through the S phase. Implications of these findings for the roles played by ABF1 and CPF1 in global regulation of mitochondrial biogenesis are discussed.

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8

Zhang, Xin, Wei Wang, Lin Shan, Le Han, Shufeng Ma, Yan Zhang, Bingtao Hao, Ying Lin, and Zhili Rong. "Gene activation in human cells using CRISPR/Cpf1-p300 and CRISPR/Cpf1-SunTag systems." Protein & Cell 9, no. 4 (November 21, 2017): 380–83. http://dx.doi.org/10.1007/s13238-017-0491-6.

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9

Lu, Wei, Xinhui Lan, Tong Zhang, Hao Sun, Sanyuan Ma, and Qingyou Xia. "Precise Characterization of Bombyx mori Fibroin Heavy Chain Gene Using Cpf1-Based Enrichment and Oxford Nanopore Technologies." Insects 12, no. 9 (September 16, 2021): 832. http://dx.doi.org/10.3390/insects12090832.

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To study the evolution of gene function and a species, it is essential to characterize the tandem repetitive sequences distributed across the genome. Cas9-based enrichment combined with nanopore sequencing is an important technique for targeting repetitive sequences. Cpf1 has low molecular weight, low off-target efficiency, and the same editing efficiency as Cas9. There are numerous studies on enrichment sequencing using Cas9 combined with nanopore, while there are only a few studies on the enrichment sequencing of long and highly repetitive genes using Cpf1. We developed Cpf1-based enrichment combined with ONT sequencing (CEO) to characterize the B. mori FibH gene, which is composed of many repeat units with a long and GC-rich sequence up to 17 kb and is not easily amplified by means of a polymerase chain reaction (PCR). CEO has four steps: the dephosphorylation of genomic DNA, the Cpf1 targeted cleavage of FibH, adapter ligation, and ONT sequencing. Using CEO, we determined the fine structure of B. moriFibH, which is 16,845 bp long and includes 12 repetitive domains separated by amorphous regions. Except for the difference of three bases in the intron from the reference gene, the other sequences are identical. Surprisingly, many methylated CG sites were found and distributed unevenly on the FibH repeat unit. The CEO we established is an available means to depict highly repetitive genes, but also a supplement to the enrichment method based on Cas9.

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Niedenthal, R., R. Stoll, and J. H. Hegemann. "In vivo characterization of the Saccharomyces cerevisiae centromere DNA element I, a binding site for the helix-loop-helix protein CPF1." Molecular and Cellular Biology 11, no. 7 (July 1991): 3545–53. http://dx.doi.org/10.1128/mcb.11.7.3545.

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The centromere DNA element I (CDEI) is an important component of Saccharomyces cerevisiae centromere DNA and carries the palindromic sequence CACRTG (R = purine) as a characteristic feature. In vivo, CDEI is bound by the helix-loop-helix protein CPF1. This article describes the in vivo analysis of all single-base-pair substitutions in CDEI in the centromere of an artificial chromosome and demonstrates the importance of the palindromic sequence for faithful chromosome segregation, supporting the notion that CPF1 binds as a dimer to this binding site. Mutational analysis of two conserved base pairs on the left and two nonconserved base pairs on the right of the CDEI palindrome revealed that these are also relevant for mitotic CEN function. Symmetrical mutations in either half-site of the palindrome affect centromere activity to a different extent, indicating nonidentical sequence requirements for binding by the CPF1 homodimer. Analysis of double point mutations in CDEI and in CDEIII, an additional centromere element, indicate synergistic effects between the DNA-protein complexes at these sites.

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Dissertations / Theses on the topic "CPF1"

1

Dowell, Simon J. "A functional analysis of yeast CPF1 and comparison with mammalian USF." Thesis, University of Oxford, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303647.

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2

Kent, Nicholas A. "Chromatin modulation in Saccharomyces cerevisiae by Centromere and Promoter Factor 1." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359458.

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3

Nissom, Peter Morin. "A structural and functional analysis of CPF1, a bHLHZIP protein of Saccharomyces cerevisiae." Thesis, University of Oxford, 1998. http://ora.ox.ac.uk/objects/uuid:93828daa-84e6-419b-ac80-1b86108a414f.

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CPF1 (centromere promoter factor 1) of Saccharomyces cerevisiae is a multifunctional protein. This multifunctionality may be associated with the different forms of the protein. To date, no clear function has been attributed to this protein. The general roles currently attributed to CPF1 are: maintenance of proper chromosome segregation or centromere function; methionine prototrophy and chromatin modulation. A series of recent papers have suggested the possibility of CPF1 being involved in protein-protein interactions. This thesis shows that CPF1 has an additional protein-protein interaction domain which is a coiled-coil. A truncation of CPF1 coding for only the C-terminal dimerization motif was cloned into a 17 expression vector and the protein, Dd, was produced in and purified from E. coli. Characterisation of the conformation of the C-terminal dimerization domain of CPF1 show the presence of a leucine zipper in Dd and that Dd forms a dimer under physiological conditions. Sequence alignment analysis of CPF1 and other bHLHZIP proteins identified a conserved serine residue in the basic domain of CPF1 and a conserved asparagine residue in the leucine zipper. Point mutations were introduced separately mutating these residues into either alanine or glutamate. The mutant alleles were cloned into yeast expression vectors. The cloned alleles were used to transform YAG93, a cpf1 deletion strain. Characterisation of the transformants obtained indicate the possibility of CPF1 being involved in the spindle assembly checkpoint control, possibly as a tethering protein. Finally, attempts were made to identify interacting partners of CPF1 and a purification scheme was designed to purify a putative CPF1 complex. Initially, a GST-CPF1 chimera was used to screen radioactively labelled crude yeast extracts for interacting partners of CPF1. This strategy identified five or six potential proteins which might form a multiprotein complex with CPFl. Attempts were then made to purify this putative complex from S. cerevisiae. The partially purified extracts were tested for CDEI activity, indicative of the presence of CPF1 in the samples and. tjhen analysed for protein content. The samples were also tested in a Holiday junction binding assay and in a histone acetyltransferase activity assay. In conclusion, CPF1 is a bHLHZIP protein that has a role in nutrient signalling and checkpoint control, possibly by acting as an intermediary protein that recruits essential factors to gene promoters or the centromere by protein-protein interactions.

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4

Cristea, Emilian, and Hassan Gelle Khalif. "Critical success factors of potential CPFR implementations : Two manufacturing case studies in Sweden based on a pre-CPFR stage from the perspective of a buyer – seller relationship." Thesis, Internationella Handelshögskolan, Högskolan i Jönköping, IHH, Centre of Logistics and Supply Chain Management (CeLS), 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:hj:diva-39767.

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Background: Higher global competition and more demanding customers force manufacturing companies to develop and adopt new collaborative strategies; the collaborative planning, forecasting and replenishment concept allows stronger supply chain cooperation, cost saving, improved efficiency and performance. Purpose: Exploratory, to study the criticality of factors that can affect the feasibility of a potential a CPFR implementation in Sweden, from the perspective of a manufacturer in a pre-CPFR implementation stage.Method: Qualitative research, using a multiple case method of two manufacturing firms operating in Sweden. Using content analysis, it revolves around studying factor criticality, all the while showing differences and commonalities in terms capabilities, and future feasibility of CPFR between the two case studies. Findings: High degree of interconnectivity between the factors; the critical success factors for Sweden are relationship and trust, goal alignment, KPI’s and costs, with very important factors such as cross-functional communication, top management support, and lower impacting factors such as IT infrastructure and change management. Relationship and trust, cross-functional communication and change management are developed factors that the Swedish manufacturing firms possess. Implications: The study’s contributions are related to the criticality of factors that can affect CPFR implementations in Sweden’s manufacturing sector, showing the importance of each, contributing academically in attempting to fill in the gap related to CPFR in Sweden, and practically by allowing better strategic decision-making in relation to future implementations. This is even more relevant due to a thorough lack of research in this area.

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Quintero, Eduardo, and Zhilin Li. "Importance of CPFR implementation in SME : Discovering the need and insights of CPFR as a supply chain strategy." Thesis, Högskolan i Gävle, Avdelningen för Industriell utveckling, IT och Samhällsbyggnad, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-13223.

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This thesis intended to evidence the need for change in a SME to reduce levels of inventory based on their process related to their supply chain management. This research focused on CPFR which is a process innovation tool that stands for collaborative planning, forecasting and replenishment (Cassivi, 2006). The idea behind this process is to make collaborative actions on all members of the supply chain to come up with a share vision and objective. Based on the core concept of this process partners along the supply chain share information based on customer trends and needs to create a single forecast that is visualize at all times by its members to react accordingly to sudden changes in demand. The research´s main objective is to describe the main process needed for CPFR implementation in actual SME that is struggling in a supply chain that is under constant pressure and obtain insight on the benefits involved in this process to reduce levels of inventory. The research was made based on the concept of CPFR through the use of databases such as Google scholar to come up with a first idea of the concept and examples of other companies implementing CPFR as a their main process for their supply chain. The second approach was to contact the companies involved in our case and use questionnaires to identify the relationship and the problems involved in their supply chain structure. This methodology was used to establish a comparison between what has been done in other companies and what is needed in our case company. The companies involved in the research are a SME and a large manufacturer. The large manufacturer is working under CPFR but with final retailers setting aside the value of incorporating upstream suppliers. Due to bullwhip effect upstream suppliers are incrementing their warehousing facilities to react to changes on the demand. This is incrementing the cost of the operation and it is creating an unstable supply. The research demonstrated companies in where CPFR is proven to reduce stockouts, markdowns, levels of inventory, time to market, strengthen the relationship and overall reduce costs. Based on the discoveries in the retail industry it was clear the benefits from this process. Companies such as Condis revealed important information based on incorporating upstream suppliers. At the end observations were made based on implementation strategies such as following the 9 step guidelines set up by VICS, developing pilot programs, reducing the number of SKU´s at the beginning, working on seasonal products rather than standard products, and developing and implementing better IT systems to manage the level of information needed.

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Pujol, Vidal Maria Magdalena. "Relació estructura-funció de les proteïnes CPT1." Doctoral thesis, Universitat de Barcelona, 2012. http://hdl.handle.net/10803/83864.

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L’enzim Carnitina palmitoïltransferasa 1 (CPT1) permet l’entrada dels àcids grassos de cadena llarga a la matriu mitocondrial per tal de ser degradats i utilitzats així com a substrat energètic. El malonil-CoA, primer intermediari en la síntesi d’àcids grassos, és un inhibidor al•lostèric de CPT1, fet que permet una regulació coordinada entre la síntesi i l’oxidació d’àcids grassos en un mateix teixit, evitant així que es donin ambdós processos alhora. Una inhibició específica de CPT1, doncs, és una bona aproximació farmacològica per al tractament de desordres metabòlics que impliquin acumulació d’àcids grassos i resistència a insulina, com la diabetis tipus 2 i l’obesitat. La present tesi doctoral s’ha centrat en l’estudi de la regulació per malonil-CoA dels isotips hepàtic (CPT1A) i muscular (CPT1B) de CPT1, així com en l’estudi de l’isotip més recentment descrit de la proteïna d’expressió en cervell, CPT1C. Per tal d’aprofundir en l’estudi de determinants moleculars de CPT1 que marquen el seu grau d’inhibició per malonil-CoA, s’han realitzat construccions mutants i delecionades dels enzims CPT1A i CPT1B i s’han expressat en el llevat “Pichia pastoris”. Mitjançant l’anàlisi del paràmetre IC50, hem demostrat que la regió aminoterminal de l’enzim CPT1A de rata (residus 1-18) exerceix un efecte dominant sobre les posicions Glu590 i Met593 en determinar la sensibilitat a la inhibició per malonil-CoA. Paral•lament, s’han generat construccions amb la seqüència de l’enzim CPT1 de porc, que presenta una gran diferència en el seu comportament enzimàtic quan es compara amb l’enzim humà, tant l’isotip hepàtic com el muscular. En aquest treball s’ha identificat l’existència d’un determinant negatiu en la posició Glu17 que explica parcialment la menor sensibilitat d’aquest enzim a la inhibició pel malonil-CoA. A més a més, s’ha construït un model 3-D in silico de la CPT1B humana, que permet justificar observacions experimentals mostrades en aquest treball, tot i que no explica encara les diferències en el comportament cinètic entre els isotips hepàtics i musculars de la proteïna. CPT1C és l’isotip descrit més recentment, i encara avui se’n desconeix la seva funció, existeix controvèrsia respecte a la seva distribució subcel•lular i no se n’han descrit els mecanismes que en regulen l’expressió. Els resultats mostrats en aquest treball demostren que CPT1C és una proteïna sense activitat enzimàtica, independentment de la característica extensió C-terminal que presenta respecte els altres isotips. D’altra banda, el patró d’expressió observat del gen Cpt1c no és compatible amb el paper que se li ha atorgat sobre la regulació de la ingesta. A més a més, hem identificat l’expressió d’una isoforma soluble de CPT1C en cervell humà adult, que ofereix una eina útil per a l’obtenció d’un cristall de la regió C-terminal citosòlica de CPT1C.
STRUCTURE-FUNCTION RELATIONSHIP OF CPT1 PROTEINS CPT1 (Carnitine palmitoyltransferase 1) enables the entry of long chain fatty acids into the mitochondrial matrix, in order to be degraded and used as energetic substrate. Malonyl-CoA, the first intermediate in the synthesis of fatty acids, is an allosteric inhibitor of CPT1. Through CPT1 inhibition, it establishes a coordinated regulation between fatty acid synthesis and oxidation, thus preventing that both pathways coexist at the same time. A specific inhibition of CPT1 is therefore a good approximation or the pharmacological treatment of metabolic disorders involving accumulation of fatty acids and nsulin resistance, such as type-2 diabetes and obesity. This thesis has focused on the study of malonyl-CoA regulation of both CPT1A and CPT1B isotypes. In addition, we studied the more recently described isotype of the protein, CPT1C. To gain insight into the study of CPT1 molecular determinants of malonyl-CoA inhibition, mutants of both CPT1A and CPT1B enzymes were expressed in yeast Pichia pastoris. Analysis of the IC50 parameter, demonstrated that the aminoterminal region (residues 1-18) of the rat CPT1A enzyme has a dominant effect over Glu590 and Met593 positions in determining its sensitivity to malonyl-CoA inhibition. We also identified the existence of a negative determinant within the sequence of pig CPT1A (position Glu17), which partially explains the low sensitivity of this enzyme to malonyl-CoA inhibition. In addition, we designed an in silico 3-D model of human CPT1B, which still does not explain the kinetic differences between liver and muscle isotypes of the protein, but justifies some of our experimental data. CPT1C is the more recently described isotype of the protein. Its function still remains unknown, and controversy exists regarding its subcellular distribution and the mechanisms that regulate its expression. Here we show that CPT1C is a protein without enzymatic activity, regardless of its characteristic C-terminal extension, compared to the other isotypes. Moreover, the observed gene expression pattern is not compatible with its given role on the regulation of food intake. In addition, we identified the expression of a soluble isoform of CPT1C in human adult brain, providing a useful tool for obtaining a crystalline structure of the cytosolic C-terminal region of CPT1.

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Takeda, Kimitoshi. "Photoreaction dynamics of Cyanobacterial phytochrome 1 (Cph1)." Kyoto University, 2019. http://hdl.handle.net/2433/242633.

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Mailliet, Joël [Verfasser]. "Structural characterisation of cyanobacterial phytochrome Cph1 / Joël Mailliet." Gießen : Universitätsbibliothek, 2011. http://d-nb.info/1063178193/34.

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Crowther, Daniel. "Cloning and characterization of Cpf1P from Schizosaccharomyces pombe." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320634.

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Stoll, Robert G. "Collaborative Planning Forecasting Replenishment (CPFR): Successful Implementation Attributes." Cleveland State University / OhioLINK, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=csu1292517604.

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Books on the topic "CPF1"

1

Authority, Financial Services. Regulation of Stakeholder Pensions: Feedback on CP61. London: Financial Services Authority, 2001.

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Canada. Chief of Force Development. Beyond transformation: The CPO1/CWO Strategic Employment Model. Ottawa: Chief of Force Development, 2012.

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Georg, Bjo rn. CPFR und elektronische Marktpla tze: Neuausrichtung der kooperativen Beschaffung. Wiesbaden: Dt. Univ.-Verl., 2005.

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Maranhão, Ricardo. CPFL 90 anos: Energia e compromisso com o desenvolvimento do Brasil. [São Paulo, Brazil]: CPFL, 2002.

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Maranhão, Ricardo. CPFL 90 anos: Energia e compromisso com o desenvolvimento do Brasil. [São Paulo, Brazil]: CPFL, 2002.

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1953-, Crum Colleen, ed. Supply chain collaboration: How to implement CPFR and other best collaborative practices. Boca Raton, FL: J. Ross Pub., 2005.

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Low, Linda. Housing a healthy, educated, and wealthy nation through the CPF. Singapore: Institute of Policy Studies, 1997.

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French, Canadian Parents for. Helping your child become bilingual: A toolkit for CPF members. [Ottawa, ON]: Canadian Parents for French, 2001.

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Authority, Financial Services. Interim Prudential Sourcebook: Friendly Societies & Insurance Companies: Feedback on CP41 and Supplementary Consultation. London: Financial Services Authority, 2001.

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Food and Agriculture Organization of the United Nations. Towards sustainable agriculture and improved food security & nutrition: CPF 2014 - 2018. Dhaka: FAO Representation in Bangladesh, 2014.

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Book chapters on the topic "CPF1"

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McMahon, Moira A., and Meghdad Rahdar. "Gene Disruption Using Chemically Modified CRISPR-Cpf1 RNA." In Methods in Molecular Biology, 49–60. New York, NY: Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0687-2_4.

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Zhang, Yingxiao, Yong Zhang, and Yiping Qi. "Plant Gene Knockout and Knockdown by CRISPR-Cpf1 (Cas12a) Systems." In Methods in Molecular Biology, 245–56. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8991-1_18.

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Yin, Xiaojia, Abhishek Anand, Paul Quick, and Anindya Bandyopadhyay. "Editing a Stomatal Developmental Gene in Rice with CRISPR/Cpf1." In Methods in Molecular Biology, 257–68. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-8991-1_19.

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Windham, Jonathan, Shailendra Sharma, Manoj Kumar Kashyap, and Sachin Rustgi. "CRISPR-Cas12a (Cpf1) and Its Role in Plant Genome Editing." In RNA-Based Technologies for Functional Genomics in Plants, 279–300. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-64994-4_13.

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Schawel, Christian, and Fabian Billing. "CPFR." In Top 100 Management Tools, 57–58. Wiesbaden: Gabler, 2009. http://dx.doi.org/10.1007/978-3-8349-8185-1_17.

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Schawel, Christian, and Fabian Billing. "CPFR." In Top 100 Management Tools, 61–63. Wiesbaden: Gabler Verlag, 2014. http://dx.doi.org/10.1007/978-3-8349-4691-1_20.

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Schawel, Christian, and Fabian Billing. "CPFR." In Top 100 Management Tools, 57–58. Wiesbaden: Gabler, 2011. http://dx.doi.org/10.1007/978-3-8349-6605-6_20.

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Schawel, Christian, and Fabian Billing. "CPFR." In Top 100 Management Tools, 62–64. Wiesbaden: Gabler Verlag, 2012. http://dx.doi.org/10.1007/978-3-8349-4105-3_20.

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Schawel, Christian, and Fabian Billing. "CPFR." In Top 100 Management Tools, 81–83. Wiesbaden: Springer Fachmedien Wiesbaden, 2017. http://dx.doi.org/10.1007/978-3-658-18917-4_21.

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Chadha, Rakesh, and J. Bhasker. "CPF Power Specification." In An ASIC Low Power Primer, 157–88. New York, NY: Springer New York, 2012. http://dx.doi.org/10.1007/978-1-4614-4271-4_9.

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Conference papers on the topic "CPF1"

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Zhang, Guishan, and Xianhua Dai. "CNN-SVR for CRISPR-Cpf1 Guide RNA Activity Prediction with Data Augmentation." In the 2019 9th International Conference. New York, New York, USA: ACM Press, 2019. http://dx.doi.org/10.1145/3314367.3314383.

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Luming, Yang, Lou Yun, and Chen Yu. "The research of CPFR model classification and factors influencing CPFR model selection." In 2013 International Conference of Information Technology and Industrial Engineering. Southampton, UK: WIT Press, 2013. http://dx.doi.org/10.2495/itie130061.

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Wang, WenJie. "Combination-forecasting modeling for CPFR collaboration." In 2011 International Conference on E-Business and E-Government (ICEE). IEEE, 2011. http://dx.doi.org/10.1109/icebeg.2011.5881294.

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Sagar, Amrit, Christopher R. Nehme, Anil Saigal, and Thomas P. James. "Validation of a Conventional Finite Element Model for Simulation of a Micropunching Process." In ASME 2014 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2014. http://dx.doi.org/10.1115/imece2014-36908.

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Abstract:

Finite element analysis (FEA) of metal microforming processes may require Crystal Plasticity Finite Element (CPFE) formulations to incorporate material inhomogeneity as feature size approaches grain size. Presently, it is unknown if the micropunching process, where holes are formed by shearing thin metal foils with a thickness on the same scale as grain size, can be accurately simulated by using the material’s bulk material properties or if CPFE is required. In the current research, validity of conventional FEA in simulating micropunching is investigated as CPFE formulations have yet to be integrated with most commercially available programs. Using DEFORM finite element software, strain hardening and strain rate hardening material models were employed to approximate flow stress when punching 200 μm diameter holes in 25 μm thick annealed copper foil. For validation of peak punching force, micro holes were fabricated with a nominal diameter of 200 μm for die clearances ranging from 7.6% to 48% of material thickness. The average grain size of the foil was determined to be approximately 47 μm. Therefore, micropunching was predominantly through a single grain across foil thickness and less than a grain in the direction of radial die clearance. Results indicate that the homogeneous material model in DEFORM is capable of predicting the maximum punching forces with reasonable accuracy, concluding that a CPFE model is not necessary for this category of micropunching. Regardless of die clearance, the maximum punching force was approximately 3 N.

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THAPA, RAJAN K., RAJAN JAISWAL, and BRITT M. E. MOLDESTAD. "CPFD SIMULATION OF A FIXED BED GASIFICATION REACTOR." In ENERGY PRODUCTION AND MANAGEMENT 2020. Southampton UK: WIT Press, 2020. http://dx.doi.org/10.2495/epm200061.

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Chi, Kai, Ning Zhang, and Lejun Chi. "The Drug Demand Forecast Model Based on CPFR." In The 6th International Conference on Electrical and Control Engineering (ICECE2015) and The 4th International Conference on Materials Science and Manufacturing (ICMSM2015). WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789813100312_0010.

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Lakshmi, M. S., Pukhraj Vaya, and Srinivasan Venkataramanan. "Power management in SoC using CPF." In 2011 3rd International Conference on Electronics Computer Technology (ICECT). IEEE, 2011. http://dx.doi.org/10.1109/icectech.2011.5941711.

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Sharma, Krishna P., and T. P. Sharma. "CPFR: Coverage preserving failure recovery in wireless sensor networks." In 2015 International Conference on Advances in Computer Engineering and Applications (ICACEA). IEEE, 2015. http://dx.doi.org/10.1109/icacea.2015.7164716.

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Zhongwen, Zhang. "The developing strategy for information system based on CPFR." In 2010 International Conference on Logistics Systems and Intelligent Management (ICLSIM). IEEE, 2010. http://dx.doi.org/10.1109/iclsim.2010.5461197.

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Liu, Yongjun, Wenjuan Ruan, and Uday Venkatadri. "RosettaNet-Based Implementation of CPFR Using Semantic Web Services." In 2009 International Conference on Management and Service Science (MASS). IEEE, 2009. http://dx.doi.org/10.1109/icmss.2009.5302981.

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Reports on the topic "CPF1"

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Schnurr, N. M. Thermal analysis of the CPFR (Confinement Physics Research Facility)/ZTH apparatus. Office of Scientific and Technical Information (OSTI), January 1989. http://dx.doi.org/10.2172/5549833.

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Cannon, Brenda D., and Victor J. Arca. Man in Simulant Testing of the Mark IV and Kappler CPF3 Protective Suits. Fort Belvoir, VA: Defense Technical Information Center, October 1999. http://dx.doi.org/10.21236/ada370776.

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Asudeh, I., S. Dehler, D. Forsyth, R. Jackson, F. Marillier, I. Reid, R. Cartwright, D. Heffler, R. Schieman, and D. Sharon. Seismic data from the Canadian Patrol Frigate Shock Trial CPF Trial Series #825. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1995. http://dx.doi.org/10.4095/205050.

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Johnson, John, Jeffrey Naber, Gordon Parker, Song-Lin Yang, Andrews Stevens, and Josh Pihl. Experimental Studies for CPF and SCR Model, Control System, and OBD Development for Engines Using Diesel and Biodiesel Fuels. Office of Scientific and Technical Information (OSTI), April 2013. http://dx.doi.org/10.2172/1097432.

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