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Dissertations / Theses on the topic 'Corynebacterium'

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Published: 4 June 2021
Last updated: 31 July 2024

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1

Barth, Enrico. "Study of the properties of channel-forming proteins of the cell walls of different Corynebacteriae." kostenfrei, 2008. http://www.opus-bayern.de/uni-wuerzburg/volltexte/2009/3632/.

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2

Netzer, Roman. "Untersuchungen zur Glykolyse und zum L-Serin-Stoffwechsel in Corynebacterium glutamicum." [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=971436797.

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3

Rönsch, Hendrik. "Untersuchungen zum Einfluss der Osmoregulation auf die Aminosäureproduktion mit Corynebacterium glutamicum." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=961195681.

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4

Wolf, Andreas. "Trehalosesynthese in Corynebacterium glutamicum." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=966630912.

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5

Oehlmann, Wulf. "Klonierung der Gene der Ribonucleotid-Reduktasen von Corynebacterium ammoniagenes und Corynebacterium glutamicum." [S.l. : s.n.], 1998. http://deposit.ddb.de/cgi-bin/dokserv?idn=956312373.

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6

Krug, Andreas. "Identifizierung und Charakterisierung eines Transkriptionsregulators der Aconitase von Corynebacterium glutamicum." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=973943815.

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7

Radmacher, Eva. "Untersuchungen zur Fettsäure- und Zellwandsynthese sowie zur Glutamatbildung mit Corynebacterium glutamicum." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=975641859.

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8

Brand, Sven. "Untersuchung der Zellhüllenstruktur von Corynebacterium glutamicum ATCC13032." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=965409864.

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9

Barckhausen, Olaf. "Nachweis eines mononuclearen Mangan-(II)-Zentrums in der Ribonucleotid-Reduktase aus Corynebacterium ammoniagenes ATCC 6872 nach Überexpression des den Metallocofaktor-codierenden nrdF-Gens im Originalstamm." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=973167335.

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10

Quesnel, David M. (David Marc). "Hydrocarbon utilization by Corynebacterium dioxydans." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23373.

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Batch fermentations of Corynebacterium dioxydans were performed using aliphatic hydrocarbons as substrates. The production of carboxylic acids was monitored throughout these fermentations. A relationship between the carbon chain length of the hydrocarbon substrate and the carboxylic acids was observed. The maximum yield of carboxylic acid was found to occur in the exponential growth phase, with the initial complete medium. Cell hydrophobicity is the proposed hypothesis for the residual hydrocarbon found in these fermentations. The addition of synthetic surfactant increased the yield of carboxylic acids, but posed problems in extraction of these acids.
In spite of a variety of different approaches, it was not possible to avoid contamination of the carboxylic acid products with residual hydrocarbon. The persistence of this residue was attributed to the hydrophobicity of the cell.
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11

McCormick, Margaret M. (Margaret Mary). "Transcriptional regulation in Corynebacterium glutamicum." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/11197.

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12

Vaid, Jagdish. "Immune responses to Corynebacterium pseudotuberculosis." Thesis, University of Surrey, 1988. http://epubs.surrey.ac.uk/848515/.

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Host-bacterium interaction in experimental pseudotuberculosis infection in guinea pigs was investigated. Quantitation of the pyogranulomatous response induced at the infection site revealed a biphasic (perhaps triphasic) pattern. The first peak observed within 5-9 days after infection (depending upon the dose) was attributed to a non specific response of the body while the second peak seen 17-21 days postinfection might be due to a specific antibacterial immunity. The responses induced at the primary and challenge sites could be modulated by various treatments including altering the dose and timing of the challenge. Reinfection occurred , although at reduced level, when previously infected guinea pigs were challenged at about the time of the second peak of granulomatous response. Immunity was evidenced by apparent failure of the challenge organisms (a double antibiotic resistant mutant of the parent strain) to reach the secondary infection sites (local lymph nodes and internal organs) due either to failure of dissemination or prompt elimination because of the ensuing immunity. The present study gave evidence that administration of C. pseudotuberculosis in guinea pigs results in a systemic, possibly bacteraemic spread of the organisms which is probably irrespective of the route of administration. It was difficult to demonstrate bacteraemia by blood or organ cultures except when animals were given very large doses (10[9] cfu) by the i/p route. In rabbits following administration of a large dose by the i/p route two peaks were observed: the first occurred within 30 minutes while the second was observed between 30-60 minutes. No organisms could be detected after 2 hours. Bacteraemia almost certainly occurred in guinea pigs following oral infection, as evidenced by the localization of the organisms at sites inoculated with tissue-damaging substances (calcium chloride, powdered glass/sand mixture, incomplete Freund's adjuvant and Tween saline) within 2 hr of oral infection. The method described for inducing secondary localization of C pseudotuberculosis in the present study could be adopted as a model for other bacterial diseases where metastatic lesions are important in pathogenesis. Immune responses to C. pseudotuberculosis infection in guinea pigs as observed in the present study are discussed with reference to other facultative intracellular bacteria of veterinary importance. Implications of the findings in natural disease of sheep and goats are discussed with suggestions about the future directions such studies might take.
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13

Abbouni, Bouziane. "Biochemische Charakterisierung der Ribonucleotid-Reduktase aus Corynebacterium glutamicum im Vergleich zum Manganenzym aus Corynebacterium ammoniagenes." [S.l. : s.n.], 1999. http://deposit.ddb.de/cgi-bin/dokserv?idn=960643478.

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14

Alves, Gabriela Batista. "Produção de porfirinas por corynebacterium diphtheriae." Universidade do Estado do Rio de Janeiro, 2009. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=3052.

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A produção de porfirinas por Corynebacterium diphtheriae é um objeto de estudo antigo, porém pouco explorado em Química e Ciências Médicas. O presente trabalho teve como objetivos comparar a influência de diferentes meios de cultura na produção de porfirinas por Corynebacterium diphtheriae, determinar a influencia da concentração do ferro na produção de porfirina, comparar quantitativamente a produção de porfirinas por diferentes amostras de bacilo diftérico e caracterizar químicamente os tipos de porfirinas produzidas. As técnicas utilizadas para isso foram a espectroscopia de absorção UV-visível e de fluorescência. O meio de cultura descrito por Mueller (1939), para a produção de toxina, se mostrou mais eficiente que o meio B de king, utilizado no diagnóstico laboratorial da difteria. A concentração de ferro no cultivo de Corynebacterium diphtheriae influencia a produção de porfirina. Altas concentrações de ferro inibem a produção de porfirina. A concentração de ferro onde a produção de porfirinas é máxima é de 0,20g/mL. Dentre as 11 amostras de bacilo diftérico estudadas, a amostra HC03, fermentadora de sacarose e toxinogênica, isolada de um caso de endocardite, foi a maior produtora de porfirina. A amostra ATCC de Corynebacterium ulcerans, não fermentadora de sacarose e toxinogênica, foi a amostra que produziu menos porfirina. Todas as 11 amostras apresentaram o mesmo perfil de espectro de fluorescência, sugerindo que a porfirina produzida seja a mesma nas amostras pesquisadas. As análises feitas para a caracterização do tipo de porfirina produzida levam a crer que esta seja uma coproporfirina. Os espectros de absorção e fluorescência não permitem porém determinar o(s) isômero(s) presente(s).
Porphyrin production by Corynebacterium diphtheriae is an old research interest but less attention has been given to the subject in the areas of chemistry and medical sciences. The object of this study is to compare the influence of different culture media in the production of porphyrins by Corynebacterium diphtheriae, to determine the iron concentration influence, to compare quantitatively the production of porphyrins by different samples of diphtheria bacilli and to characterize the porphyrins identities. UV-visible absorption and fluorescence spectroscopies were chosen as analytical tools. The culture medium described by Mueller (1939), for the production of toxin, was more efficient than the medium B of King, used in the laboratory diagnosis of diphtheria. The iron concentration in the cultivation of Corynebacterium diphtheriae influence the production of porphyrin. High concentrations inhibit their production. The iron concentration for maximum production of porphyrins is 0.20  g / mL. Among the 11 studied diphtheria bacillus, the sample HC03, fermented sucrose and toxinogenic, isolated from a case of endocarditis, was the largest producer of porphyrin. The sample of Corynebacterium ulcerans ATCC, unfermented sucrose and toxinogenic was the sample that produced less. All 11 samples showed the same profile of the fluorescence spectrum, suggesting that the porphyrin produced is the same in the samples studied. The tests made to characterize the type of porphyrin produced suggests that this is a coproporphyrin. However, the absorption and fluorescence spectra can not determine which isomer(s) is(are) present
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15

Ngai, Cheung. "Usefulness of molecular targets for identification of medically important tsukamurella species." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42925307.

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16

Georgi, Tobias. "Regulation der Zuckerverwertung in Corynebacterium glutamicum." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=980897343.

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17

Kabus, Armin. "Energie- und Redoxstoffwechsel von Corynebacterium glutamicum." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=981755674.

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18

Özcan, Nuran. "Anpassung an Kältestress in Corynebacterium glutamicum." [S.l.] : [s.n.], 2007. http://deposit.ddb.de/cgi-bin/dokserv?idn=983809267.

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19

Pethick, Florence Elizabeth. "Comparative genomic analyses of Corynebacterium pseudotuberculosis." Thesis, University of Glasgow, 2013. http://theses.gla.ac.uk/4287/.

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This study set out to sequence the genome of Corynebacterium pseudotuberculosis (Cp) 3/99-5, an ovine strain isolated from a naturally-occurring case of caseous lymphadenitis (CLA) in Scotland. The isolate was sequenced and assembled by 454 Life Sciences, and then gap closure performed by ‘PCR bridging’. The resulting sequence consisted of three contigs with a length of 2,319,079 bp and a G+C content of 52.18%. The genome was then annotated and predicted to contain 2,153 coding sequences. Analysis of the coding sequences revealed the presence of several putative virulence factors, including four sortases with multiple sortase target proteins containing LPXTG motifs. A further two Cp strains, an Australian ovine and a North American equine isolate, as well as C. ulcerans NCTC 12077 were sequenced for comparison. Comparative genomics, both intra- and inter-species showed all the genomes to be highly homologous. However, the C. ulcerans genome is larger than the Cp genomes and is more distinct; it was found to be more similar to the equine Cp 1/06-A isolate which is the most diverged of the Cp isolates. Phylogenetic analyses of the Corynebacterium genus were performed using house-keeping loci but also secreted protein loci from Cp 3/99-5. Bayesian analysis of house-keeping loci distinguished the bacteria to a species level. Inclusion of secreted protein loci did not distinguish the isolates any further. The main objective of this work was to utilise the Cp genome sequence to identify potential diagnostic targets which could be used to augment the available ELITEST CLA or replace it. The ELITEST CLA is the only diagnostic test for CLA that exists on the commercial market in the UK. However, due to low specificity and sensitivity, it is only operated on a flock/group basis. Analyses of the Cp 3/99-5 genome identified several potential diagnostic candidates and seven protein targets were investigated further. Attempts were made to express these candidates as recombinant proteins, however, only two recombinants were successfully expressed and purified, Cp3995_0570 and CP40. The seroreactivity of these were then assessed by IgG ELISA using a panel of ten positive and ten negative CLA ovine sera. The sera were previously defined as positive or negative by PLD and whole cell ELISAs; both of which showed a significant difference between sera types. However, neither Cp3995_0570 nor CP40 distinguished between sera originating from Cp-infected and Cp-naïve animals.
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20

Nolden, Lars. "Mechanismen der Stickstoff-Kontrolle in Corynebacterium glutamicum." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=96363609X.

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21

Krings, Eva. "Genomweite Effekte des Transkriptionsregulators LysG in Corynebacterium glutamicum." [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=970111223.

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22

Haier, Birgit. "Funktionelle Analyse des Lysin-Exportcarriers aus Corynebacterium glutamicum." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=962023566.

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23

Weinand, Martin. "Expressionsregulation von Transportern kompatibler Solute in Corynebacterium glutamicum." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972517790.

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24

Ley, Oliver Alexander. "Charakterisierung der Regulation des Prolinbiosyntheseweges in Corynebacterium glutamicum." [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=974307394.

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25

Marcondes, André Guaragna. "Micobacteriose de ovinos (Ovis aries) do Estado de São Paulo, Brasil. Correlação entre teste imunoalérgico, cultivo e histopatológico." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-27062007-130244/.

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A escassez de dados sobre tuberculose e micobacterioses em ovinos (Ovis aries) motivou o presente trabalho de isolamento e tipificação de microorganismos presentes em linfonodos e lesões macroscópicas sugestivas de tuberculose. Foram avaliados pelo teste tuberculinico, 353 ovinos das raças Santa Inês e Texel de duas propriedades da região de Pindamonhangaba - São Paulo. Dos 57 animais selecionados para abate, 31 apresentavam reação ao PPD bovino maior que ao PPD aviário e 26 com reação ao PPD bovino menor que ao PPD aviário. Onze animais (19,3%) apresentaram na necrópsia lesões sugestivas de tuberculose. Os órgãos afetados foram o fígado, linfonodo submandibular, intestino, pulmão, linfonodo mediastino e glândula mamária. Foram isoladas micobactérias de sete (12,3%) animais e a tipificação genética pelo método de PRA demonstrou cinco (71,42%) infectados pelo Mycobacterium flavescens 1, um (14,28%) pelo M. kansasi, e um (14,28%) por micobactéria pertencente ao Complexo M. tuberculosis. Exames bacteriológicos para outras bactérias e/ou fungos isolaram Corynebacterium pseudotuberculosis em quatro (7,01%) dos 57 animais abatidos. Houve isolamento simultâneo de micobactérias e de Corynebacterium pseudotuberculosis em dois (3,5%) dos 57 animais abatidos. Os exames histopatológicos apontaram em nove (15,78%) animais a presença de granuloma e coloração de Ziehl-Neelsen positivo. A análise dos resultados obtidos permitiram concluir que, neste trabalho, os testes imunoalérgicos (Teste Cervical Simples e Teste Cervical Comparativo) não foram capazes de diferenciar infecção provocada pelo M. flavescens 1, M. kansasi, complexo M. tuberculosis e C. pseudotuberculosis. Nos exames macroscópico e histopatológico lesões provocadas por M. flavescens 1, M. kansasi, e C. pseudotuberculosis não foram diferenciáveis das provocadas pelo complexo M. tuberculosis.
The occurrence of few data on ovine (Ovis aries) tuberculosis and mycobacteriosis has motivated this work of isolation and typing microorganism found in lymph nodes and tuberculosis-like gross lesions. Tuberculin skin test was performed in 353 Santa Ines and Texel ovine breeds of two properties located at Pindamonhangaba Municipality - Sao Paulo State. Fifty seven animals were selected to be slaughtered and 31 of them had the bovine PPD skin test higher than avian PPD and other 26 presented bovine PPD reaction lower than avian PPD. Eleven animals (19.3%) showed tuberculosis-like gross lesions at necropsy. Most affected organs were liver, submandibular lymph nodes, intestines, lungs, mediastinic lymph nodes and mammary gland. It was possible to isolate mycobacteria from seven (12.3%) animals and genetic typing by the PRA method showed that five animals (71.42%) were infected with Mycobacterium flavescens 1, one (14.28%) with M. kansasi, and one (14.28%) with M. tuberculosis complex mycobacteria. Bacteriological culture isolation for other bacteria and/or fungi were positive for Corynebacterium pseudotuberculosis in four (7.01%) of 57 slaughtered animals. There was a concomitant isolation of mycobacteria and Corynebacterium pseudotuberculosis in two (3.5%) of 57 slaughtered animals. Histopathologic examination demonstrated the presence of granuloma and positive Ziehl-Neelsen staining in nine (15.78%) animals. Results analysis allowed concluding that in this work, immuno-allergic tests (Simple Cervical Test and Comparative Cervical Test) were not capable to differentiate infection caused by M. flavescens 1, M. kansasi, M. tuberculosis complex and C. pseudotuberculosis. During gross examination and histopathology, lesions caused by M. flavescens 1, M. kansasi, and C. pseudotuberculosis were not distinguishable from those caused by M. tuberculosis complex.
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26

Wessel, Mirja. "Funktionelle Analyse des essentiellen Zweikomponenten-Signaltransduktionssystems CgtSR4 aus Corynebacterium glutamicum." [S.l.] : [s.n.], 2003. http://deposit.ddb.de/cgi-bin/dokserv?idn=971436320.

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27

Engels, Sabine. "Regulation der clp-Genexpression durch ClgR und Definition des ClgR-Regulons aus Corynebacterium glutamicum." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=973949759.

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28

Burger, Udo. "Struktur- und Funktionsanalysen am osmotisch regulierten Transporter BetP aus Corynebacterium glutamicum." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964128926.

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29

Berens, Stephan. "Untersuchung des Proteinexports in Corynebacterium glutamicum anhand der Produktion heterologer Exoenzyme." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=960161244.

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30

Meier-Wagner, Jana Sheila. "Mechanismen und Regulation der (Methyl- )Ammoniumaufnahme in Corynebacterium glutamicum." [S.l. : s.n.], 2000. http://deposit.ddb.de/cgi-bin/dokserv?idn=960649220.

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31

Rübenhagen, René. "Der Glycinbetain-Transporter BetP aus Corynebacterium glutamicum als Osmosensor." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=96230011X.

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32

Steger, Ralf. "Vergleichende Studien zur Aktivitätsregulation osmosensitiver Transporter aus Corynebacterium glutamicum." [S.l. : s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=96663117X.

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33

Strelkov, Sergey. "Entwicklung und Anwendung einer Methode zur Metabolomanalyse von Corynebacterium glutamicum." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=97235963X.

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34

Elhariry, Hesham. "Characterization of a thermosensitive ribonucleotide reductase mutant derived from Corynebacterium ammoniagenes ATCC 6872 and its use in the production of nucleotides." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972361324.

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35

Botzenhardt, Johannes. "Regulation des Betaintransporters BetP aus Corynebacterium glutamicum während der Anpassung an hyperosmotischen Stress." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=971846413.

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36

Бєлкін, Євгеній Сергійович. "Застосування Corynebacterium glutamicum для отримання глутамінової кислоти." Магістерська робота, Київський національний університет технологій та дизайну, 2021. https://er.knutd.edu.ua/handle/123456789/19232.

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Дипломну магістерську роботу присвячено вивченню Corynebacterium glutamicum, їх властивостей, практичного застосування. У дипломній роботі обґрунтовано технологію виробництва глутамінової кислоти, отриманої на основі Corynebacterium glutamicum. Представлено технологічну схему виробництва глутамінової кислоти, яка передбачає стадії ферментації, осадження надлишкових іонів, концентрування, кристалізації, фільтрування і висушування кристалів глутамінової кислоти. Обґрунтовано вибір технологічного обладнання для реалізації виробництва. Дипломна робота включає методики контролю стадій виробництва глутамінової кислоти.
The master's thesis is devoted to the study of Corynebacterium glutamicum, their properties, practical application. The thesis substantiates the technology of production of glutamic acid obtained on the basis of Corynebacterium glutamicum. The technological scheme of glutamic acid production is presented, which provides for the stages of fermentation, precipitation of excess ions, concentration, crystallization, filtration and drying of glutamic acid crystals. The choice of technological equipment for the realization of production is substantiated.
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37

Kensley, Joy A. "Glycogen metabolism in Corynebacterium glutamicum ATCC 13032." Doctoral thesis, University of Cape Town, 2004. http://hdl.handle.net/11427/4279.

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Includes bibliographical references (leaves 108-123).
Corynebacterium glutamicum is a Gram-positive facultative aerobe particularly known for its industrial application in the synthesis of amino acids, such as L-glutamate and Llysine. The central metabolic pathways of this organism has been an area of much research by many groups. Linked to glycolysis is the synthesis of glycogen, previously considered a storage molecule of excess glucose. No information concerning the role of glycogen or its metabolism in C. glutamicum was known, and the aim of this work was to elucidate glycogen metabolism in this industrially important organism.
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38

Schulz, Anton A. "Nitrogen metabolism in Corynebacterium glutamicum ATCC 13032." Doctoral thesis, University of Cape Town, 2002. http://hdl.handle.net/11427/4329.

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Bibliography: leaves 125-146.
Corynebacterium glutamicum is extensively used for the commercial production of a host of amino acids including lysine, glutamate, and threonine. Consequently, much research has been directed at analyzing nitrogen metabolism in this bacterium. In particular, our research focused on investigating the regulation of nitrogen assimilation. Initially, we searched for homologs of the Streptomyces glnR, glnII, and glnE genes in C. glutamicum. These studies, however, were met with limited success, and we therefore decided to use promoter probe vectors in order to identify nitrogen-responsive promoters.
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39

Weerasekera, Dulanthi [Verfasser], Andreas [Akademischer Betreuer] Burkovski, Andreas [Gutachter] Burkovski, and Paul [Gutachter] Hoskisson. "Characterization of virulence factors of Corynebacterium diphtheriae and Corynebacterium ulcerans / Dulanthi Weerasekera ; Gutachter: Andreas Burkovski, Paul Hoskisson ; Betreuer: Andreas Burkovski." Erlangen : Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 2019. http://d-nb.info/1194650848/34.

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40

Gande, Roland. "Untersuchungen zur Lipid- und Zellwandsynthese in Corynebacterium glutamicum." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=979441153.

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41

Sorger-Herrmann, Ulrike. "Analyse des Mechanismus der Phosphatregulation in Corynebacterium glutamicum." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=98228859X.

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42

Stolz, Michael. "Untersuchungen zur L-Serin-Bildung mit Corynebacterium glutamicum." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=982789254.

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43

Brockmann-Gretza, Olaf. "Globale Expressionsanalyse der Stringenten Kontrolle in Corynebacterium glutamicum." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=983421285.

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44

Laslo, Tanja [Verfasser]. "D-arabitol metabolism of Corynebacterium glutamicum / Tanja Laslo." Ulm : Universität Ulm. Fakultät für Naturwissenschaften, 2013. http://d-nb.info/1036215148/34.

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45

Bolt, Frances. "The population structure of the Corynebacterium diphtheriae group." Thesis, University of Warwick, 2009. http://wrap.warwick.ac.uk/1759/.

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A multi-locus sequence typing scheme was designed for the Corynebacterium diphtheriae group comprising C. diphtheriae, C. ulcerans and C. pseudotuberculosis. Five MLST genes (atpA, dnaE, fusA, odhA and rpoB) were analysed to resolve the inter-species relationships of the three organisms. No alleles were shared between the species and MLSA analysis indicated that they represent distinct populations with no evidence of recombination between the organisms. One hundred and fifty four C. diphtheriae isolates were analysed by MLST using the same five loci and two additional genes (dnaK and leuA). The data derived was in concordance with previous ribotyping studies and identified two clonal complexes associated with diphtheria outbreaks. No correlation between ST and toxin production or clinical presentation was observed. In contrast to the biovars gravis, mitis and intermedius the atypical belfanti strains were distinct by phylogenetic analysis. Although two C. diphtheriae cultures isolated from a horse clustered with the human strains four isolates obtained from two cats shared no alleles with the other isolates examined. MLST analysis of 69 C. ulcerans isolates using the five MLST genes and the virulence determinant phospholipase D (PLD) revealed that veterinary and human isolates were not genetically distinct. As with the C. diphtheriae population recombination was shown to play an important role in the evolution of the organism. The 73 C. pseudotuberculosis isolates were examined with the five MLST loci, pld and two genes within the Fe acquisition operon; fagCand fagD. The nitrate negative and positive strains were distinguished by MLST but shared ancestry was apparent as the same alleles were identified in two to three of the genes. MLST supported previous studies which indicate that C. pseudotuberculosis biovar ovis is a genetically homogenous species. This is the first MLST scheme designed for the C. diphtheriae group and the first to encompass multiple species within the Corynebacterium genus. MLST provides a useful tool for the discrimination of the three species and will enable the detection of genetic exchange events between and within the organisms.
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46

Edwards, Nicholas. "The production of L-lysine by Corynebacterium glutamicum." Thesis, University of Kent, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317596.

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47

De, Zoysa Aruni Shanika. "Transcontinental epidemiology and molecular characterisation of Corynebacterium diphtheriae." Thesis, University of Leeds, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.401083.

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48

Theron, Grant de V. "L-arginine overproduction in Corynebacterium glutamicum ATCC 13032." Doctoral thesis, University of Cape Town, 2009. http://hdl.handle.net/11427/12122.

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Corynebacterium glutamicum is widely used for the commercial production of a variety of amino acids, including L-lysine, L-glutamate and L-threonine. With the exception of Larginine, the biosynthesis and regulation of most of these compounds in this bacterium are relatively well characterised in the literature. The research presented here focuses on improving our understanding of the regulation of L-arginine biosynthesis in C. glutamicum. This was performed with the ultimate goal of creating strains capable of producing L-arginine commercially. A novel gene replacement system was initially used for the directed mutation of the Larginine biosynthetic gene cluster in C. glutamicum ATCC 13032. This was met with limited success, however, and the pK19mobsacB vector was thus adopted for further mutagenesis of this region.
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de, V. Theron Grant. "L-Arginine Overproduction in Corynebacterium glutamicum ATCC 13032." Doctoral thesis, University of Cape Town, 2009. http://hdl.handle.net/11427/3069.

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50

Seibold, Gerd Michael. "Charakterisierung des Glycogen- und Maltosestoffwechsels von Corynebacterium glutamicum." [S.l. : s.n.], 2008. http://nbn-resolving.de/urn:nbn:de:bsz:289-vts-63818.

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