Academic literature on the topic 'Conformational characterization'

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Journal articles on the topic "Conformational characterization"

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Seidler, Clarissa A., Janik Kokot, Monica L. Fernández-Quintero, and Klaus R. Liedl. "Structural Characterization of Nanobodies during Germline Maturation." Biomolecules 13, no. 2 (2023): 380. http://dx.doi.org/10.3390/biom13020380.

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Camelid heavy-chain antibody variable domains (VHH), nanobodies, are the smallest-known functional antibody fragments with high therapeutic potential. In this study, we investigate a VHH binding to hen egg-white lysozyme (HEL). We structurally and dynamically characterized the conformational diversity of four VHH variants to elucidate the antigen-binding process. For two of these antibodies, not only are the dissociation constants known, but also the experimentally determined crystal structures of the VHH in complex with HEL are available. We performed well-tempered metadynamics simulations in
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Raich, Ivan, Karel Pauk, Ales Imramovsky, and Josef Jampílek. "Conformational and Chiroptical Properties of Salicylamide-Based Peptidomimetics." Symmetry 16, no. 2 (2024): 138. http://dx.doi.org/10.3390/sym16020138.

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Optical rotation (OR), the most frequently used chiroptical method, is used for the characterization of newly synthesized or isolated compounds. Computational predictions of OR are, however, mainly used for the determination of the absolute configurations of chiral compounds, but they may also be used for the verification of conformational analysis results if the experimental values are known. Our computational study deals with the conformational analysis of flexible salicylamide-based peptidomimetics, starting with a conformation search, then a low-level ab initio preoptimization of the hundr
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Paz, Aviv, Derek P. Claxton, Jay Prakash Kumar, et al. "Conformational transitions of the sodium-dependent sugar transporter, vSGLT." Proceedings of the National Academy of Sciences 115, no. 12 (2018): E2742—E2751. http://dx.doi.org/10.1073/pnas.1718451115.

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Sodium-dependent transporters couple the flow of Na+ ions down their electrochemical potential gradient to the uphill transport of various ligands. Many of these transporters share a common core structure composed of a five-helix inverted repeat and deliver their cargo utilizing an alternating-access mechanism. A detailed characterization of inward-facing conformations of the Na+-dependent sugar transporter from Vibrio parahaemolyticus (vSGLT) has previously been reported, but structural details on additional conformations and on how Na+ and ligand influence the equilibrium between other state
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Dong, Qiwen, Kai Wang, Bin Liu, and Xuan Liu. "Characterization and Prediction of Protein Flexibility Based on Structural Alphabets." BioMed Research International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/4628025.

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Motivation.To assist efforts in determining and exploring the functional properties of proteins, it is desirable to characterize and predict protein flexibilities.Results.In this study, the conformational entropy is used as an indicator of the protein flexibility. We first explore whether the conformational change can capture the protein flexibility. The well-defined decoy structures are converted into one-dimensional series of letters from a structural alphabet. Four different structure alphabets, including the secondary structure in 3-class and 8-class, the PB structure alphabet (16-letter),
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SENGE, MATHIAS O., INES BISCHOFF, NORA Y. NELSON, and KEVIN M. SMITH. "Synthesis, Reactivity and Structural Chemistry of 5,10,15,20-Tetraalkylporphyrins." Journal of Porphyrins and Phthalocyanines 03, no. 02 (1999): 99–116. http://dx.doi.org/10.1002/(sici)1099-1409(199902)3:2<99::aid-jpp109>3.0.co;2-6.

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The synthesis, reactivity and full characterization of a series of meso-tetraalkyl porphyrins and metalloporphyrins with R ≡ n-butyl (6), 2-methyl-propyl (7), isopropyl (8), l-ethyl-propyl (10) and tert-butyl (11) groups are reported. Derivatives of the last of these show considerably bathochromically shifted absorption bands and the crystal structure of Zn ( II )11(pyr) exhibits a severely ruffled macrocycle conformation. Systematic crystallographic studies of the porphyrins showed that the free base porphyrins with R ≡ n-butyl ( H 26), 2-methyl-propyl ( H 27) and 1-ethyl-propyl ( H 210) are
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Raisinghani, Nishank, Vedant Parikh, Brandon Foley, and Gennady Verkhivker. "AlphaFold2-Based Characterization of Apo and Holo Protein Structures and Conformational Ensembles Using Randomized Alanine Sequence Scanning Adaptation: Capturing Shared Signature Dynamics and Ligand-Induced Conformational Changes." International Journal of Molecular Sciences 25, no. 23 (2024): 12968. https://doi.org/10.3390/ijms252312968.

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Proteins often exist in multiple conformational states, influenced by the binding of ligands or substrates. The study of these states, particularly the apo (unbound) and holo (ligand-bound) forms, is crucial for understanding protein function, dynamics, and interactions. In the current study, we use AlphaFold2, which combines randomized alanine sequence masking with shallow multiple sequence alignment subsampling to expand the conformational diversity of the predicted structural ensembles and capture conformational changes between apo and holo protein forms. Using several well-established data
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Moutusi, Manna, та K. Murarka Rajesh. "Characterization of conformational ensemble of C-terminally truncated α-synuclein in aqueous solution by atomistic molecular dynamics simulations". Journal of Indian Chemical Society Vol. 96, Jul 2019 (2019): 991–1002. https://doi.org/10.5281/zenodo.5644801.

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Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhopal-462 066, Madhya Pradesh, India <em>E-mail:</em> moutusi.manna.chem@gmail.com, rkm@iiserb.ac.in <em>Manuscript received online 30 April 2019, revised and accepted 28 May 2019</em> &alpha;-Synuclein is an intrinsically disordered protein, whose aggregation into amyloid fibril is involved in the pathology of Parkinson&rsquo;s disease. C-terminally truncated &alpha;-synuclein variants, which are naturally generated from the full-length &alpha;-synuclein, are enriched in the pathological &
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Siddiqui, Mahtab Z. "Dehydrophenylalanine analogues: conformational characterization." International Journal of Biological Macromolecules 26, no. 1 (1999): 17–21. http://dx.doi.org/10.1016/s0141-8130(99)00063-x.

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Dluhy, R. A., D. Moffatt, D. G. Cameron, R. Mendelsohn, and H. H. Mantsch. "Characterization of cooperative conformational transitions by Fourier transform infrared spectroscopy: application to phospholipid binary mixtures." Canadian Journal of Chemistry 63, no. 7 (1985): 1925–32. http://dx.doi.org/10.1139/v85-319.

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Temperature-induced cooperative conformational transitions in biopolymers may be examined in aqueous solution by the use of Fourier transform infrared spectroscopy. The transition is described in terms of a two-state model with a fractional population parameter (which describes the degree of transition at various values intermediate between the two limiting cases) that can be generated by a least-squares technique. This procedure allows for calculation of a conformational index without assuming a linear dependence of the infrared parameter on the extent of transition. Calculations based on the
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Mountassif, Driss, Lucien Fabre, Kaustuv Basu, Mihnea Bostina, Slavica Jonic, and Isabelle Rouiller. "Conformational heterogeneity of the AAA ATPase p97 characterized by single particle cryo-EM." Acta Crystallographica Section A Foundations and Advances 70, a1 (2014): C853. http://dx.doi.org/10.1107/s2053273314091463.

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p97, a member of the AAA (ATPase Associated with various Activities) ATPase family, is essential and centrally involved in a wide variety of cellular processes. Single amino-acid substitutions in p97 have been associated with the severe degenerative disorder of Inclusion Body Myopathy associated with Paget disease of bone and Frontotemporal Dementia (IBMPFD) as well as amytropic leteral sclerosis (ALS). Current models propose that p97 acts as a motor transmitting the energy from the ATPase cycle to conformational changes of substrate protein complexes causing segregation, remodeling or translo
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Dissertations / Theses on the topic "Conformational characterization"

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Ambrogi, Martina. "Synthesis, characterization and conformational studies of arylbenzylmaleimides." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amslaurea.unibo.it/4251/.

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From the discoveries of Pasteur, stereochemistry has played an increasingly important role in the chemical sciences. In particular conformational study of molecules with axial chirality is object of intense research. Through Dynamic-NMR analysis and simulation of the spectra, the energy rotational barriers value of conformers are obtained. When this barrier is high sufficiently, atropisomeric stable compounds can be reached. They can be separated and used in stereo-synthesis and in packing processes. 3,4-bis-aryl maleimides, in which the aromatic groups are sufficiently bulky, generate atropi
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Silenzi, Ilaria. "Synthesis, characterization and conformational studies of bis-phenothiazine-aryl-boranes." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2019. http://amslaurea.unibo.it/19201/.

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This thesis project presents a work based on the study of a particular class of amino-boranes, called bis-phenothiazine-aryl-boranes. The peculiarity of these compounds is the N-B-N chemical moiety and their complex conformational behaviour, due to the combination of steric hindrance and conjugation of the B-N bond. Our work is focused on two main products with different symmetry: bis-phenothiazine-2-methylnaphthyl-borane (2b) and bis-phenothiazine-anthracenyl-borane (2c). We firstly focused our attention on an effective way of synthesis, by optimizing both reaction conditions and purification
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Zych, Andrew John. "Conformational characterization of abiotic secondary structure based on aromatic stacking /." Full text (PDF) from UMI/Dissertation Abstracts International, 2001. http://wwwlib.umi.com/cr/utexas/fullcit?p3008484.

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Foschi, Simone. "Synthesis, Characterization and Conformational Studies of Modified Carbazole-bis-aryl-boranes." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2020. http://amslaurea.unibo.it/20686/.

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During this project we have synthetized different compounds belonging to the class of amino-boranes for the study of bis-aryl-B=N system. We have decided to keep unchanged the aryl components and change only the amine to observe the effect of that on the B=N bond. The used amines are modified carbazoles with functional groups chosen to amplify or disempower the steric and the conjugation effect. We have found that the evaluation of steric barrier was possible studying the gearing aryls rotation around the C-B bonds, while the conjugation barrier is instead given by the energy needed to break t
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Croasdale, Rebecca Alice. "Characterization of the conformational dynamics of the Nek2 leucine zipper domain." Thesis, University of Leicester, 2013. http://hdl.handle.net/2381/27799.

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Nek2 is a cell cycle regulated protein kinase. Its expression and activity peak in S and G2 phase before the protein is targeted for degradation in mitosis. Nek2 activity promotes centrosome separation and bipolar spindle formation, processes that are essential for maintaining the fidelity of chromosome segregation and cell division. Importantly, Nek2 inhibition results in increased apoptosis and senescence in cancer cell lines and tumour xenografts making Nek2 an attractive target for chemotherapeutic intervention. The α-helix is the most common secondary structure in proteins, with 2-3% of a
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Ieronymaki, Matthaia. "Immunological and Conformational characterization of synthetic peptide probes for autoimmune diseases." Thesis, Cergy-Pontoise, 2016. http://www.theses.fr/2016CERG0831/document.

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Les maladies auto-immunes sont des maladies chroniques et hétérogènes caractérisées par des réactions du système immunitaire acquis contre les propres tissus sains de l'organisme. Ces maladies affectent presque 5% de la population mondiale et en particulier les jeunes adultes. La complexité de leur spectre est énorme et même si leur étiologie est encore incertaine, il a été démontré que des facteurs génétiques et environnementaux sont impliqués dans le déclenchement du mécanisme pathologique. Cependant, il est nécessaire d'utiliser des outils diagnostiques et / ou pronostiques fiables pour le
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Fisher, Charles. "Statistical Characterization of Protein Ensembles." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10223.

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Conformational ensembles are models of proteins that capture variations in conformation that result from thermal fluctuations. Ensemble based models are important tools for studying Intrinsically Disordered Proteins (IDPs), which adopt a heterogeneous set of conformations in solution. In order to construct an ensemble that provides an accurate model for a protein, one must identify a set of conformations, and their relative stabilities, that agree with experimental data. Inferring the characteristics of an ensemble for an IDP is a problem plagued by degeneracy; that is, one can typically const
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Niyogi, Sandip. "Synthesis and characterization of molecules to study the conformational barriers of fluorocarbon chains." Thesis, University of North Texas, 2000. https://digital.library.unt.edu/ark:/67531/metadc2511/.

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Fluorocarbons are known to be stiffer than their hydrocarbon analogues, a property that underlines the extensive industrial application of fluorocarbon materials. Although there has been previous studies on the rotational barrier of molecules having fluorocarbon centers, a detailed systematic study is necessary to quantify flurocarbon stiffness. The molecules, Pyrene-(CF2)n-Pyrene, Pyrene-(CF2)n-F, Pyrene-(CH2)n-Pyrene and Pyrene-(CH2)n-H were therefore synthesized to enable the determination of the barrier to rotation of the carbon backbone in fluorocarbons. Conformational studies will be com
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Cherwa, Jr James Edward. "Characterization of Scaffolding Proteins Altered in the Ability to Perform a Critical Conformational Switch." Diss., The University of Arizona, 2009. http://hdl.handle.net/10150/195476.

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Throughout recent history scientists have struggled to elucidate the biochemical and biophysical mechanisms that guide the assembly of macromolecular structures. The early models of "sub-assembly" or "self assembly" attempted to explain how individual components could interact in a precisely regulated manner to form higher-ordered complex biological structures. Subsequent studies, using viral systems as assembly models, demonstrated how protein-protein and protein-nucleic acid interactions assist in lowering the thermodynamic barriers that typically disfavor assembly.Due to their simplicity, v
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Braggin, Greg A. "Effect of Surfactant Architecture on Conformational Transitions of Conjugated Polyelectrolytes." DigitalCommons@CalPoly, 2015. https://digitalcommons.calpoly.edu/theses/1411.

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Water soluble conjugated polyelectrolytes (CPEs), which fall under the category of conductive polymers, possess numerous advantages over other conductive materials for the fabrication of electronic devices. Namely, the processing of water soluble conjugated polyelectrolytes into thin film electronic devices is much less costly as compared to the processing of inorganic materials. Moreover, the handling of conjugated polyelectrolytes can be performed in a much more environmentally friendly manner than in the processing of other conjugated polymers because conjugated polyelectrolytes are water s
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Books on the topic "Conformational characterization"

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(Editor), Ying Xu, Dong Xu (Editor), and Jie Liang (Editor), eds. Computational Methods for Protein Structure Prediction and Modeling 1: Basic Characterization (Biological and Medical Physics, Biomedical Engineering). Springer, 2006.

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Maher, Christopher J., and Elaine R. Mardis. Genomic Landscape of Cancer. Oxford University Press, 2017. http://dx.doi.org/10.1093/oso/9780190238667.003.0004.

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The study of cancer genomics has advanced rapidly during the last decade due to the development of next generation or massively parallel technology for DNA sequencing. The resulting knowledge is transforming the understanding of both inherited (germline) genetic susceptibility and the somatic changes in tumor tissue that drive abnormal growth and progression. The somatic alterations in tumor tissue vary depending on the type of cancer and its characteristic “genomic landscape.” New technologies have increased the speed and lowered the cost of DNA sequencing and have enabled high-volume charact
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Book chapters on the topic "Conformational characterization"

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Franks, Felix. "Conformational Stability Denaturation and Renaturation." In Characterization of Proteins. Humana Press, 1988. http://dx.doi.org/10.1007/978-1-59259-437-5_4.

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Fuad, Fazia Adyani Ahmad. "Characterization of Conformational and Oligomeric States of Proteins." In Multifaceted Protocol in Biotechnology. Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2257-0_14.

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Delaforge, Elise, Tiago N. Cordeiro, Pau Bernadó, and Nathalie Sibille. "Conformational Characterization of Intrinsically Disordered Proteins and Its Biological Significance." In Modern Magnetic Resonance. Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-28275-6_52-1.

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Delaforge, Elise, Tiago N. Cordeiro, Pau Bernadó, and Nathalie Sibille. "Conformational Characterization of Intrinsically Disordered Proteins and Its Biological Significance." In Modern Magnetic Resonance. Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-28388-3_52.

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Pinto, Julia C., Jean Jacques Bourguignon, and Camille-Georges Wermuth. "Characterization of the pharmacophoric pattern of acetylcholinesterase inhibitors through conformational analysis." In Trends in QSAR and Molecular Modelling 92. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1472-1_104.

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Langer, Thierry, and Camille-Georges Wermuth. "Prolyl-endopeptidase inhibitors: Pharmacophore characterization via conformational analysis and pattern recognition." In Trends in QSAR and Molecular Modelling 92. Springer Netherlands, 1993. http://dx.doi.org/10.1007/978-94-011-1472-1_105.

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Xiao, Jianxi. "Conformational and Dynamic Characterization of Collagen Mimic Peptides by NMR Spectroscopy." In Springer Series in Biomaterials Science and Engineering. Springer Nature Singapore, 2024. http://dx.doi.org/10.1007/978-981-10-1941-8_7.

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Hamrick, Grayson S., Casey H. Londergan, and Louise K. Charkoudian. "Heterologous Expression, Purification, and Characterization of Type II Polyketide Synthase Acyl Carrier Proteins." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2273-5_13.

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AbstractThe enzymes that comprise type II polyketide synthases (PKSs) are powerful biocatalysts that, once well-understood and strategically applied, could enable cost-effective and sustainable access to a range of pharmaceutically relevant molecules. Progress toward this goal hinges on gaining ample access to materials for in vitro characterizations and structural analysis of the components of these synthases. A central component of PKSs is the acyl carrier protein (ACP), which serves as a hub during the biosynthesis of type II polyketides. Herein, we share methods for accessing type II PKS A
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Baniak, Edmund L., Lila M. Gierasch, Jean Rivier, and Arnold T. Hagler. "NMR analysis and conformational characterization of cyclic antagonists of gonadotropin-releasing hormone." In Peptides. Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-010-9595-2_134.

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Ogden, Dylan, and Mahmoud Moradi. "Molecular Dynamics–Based Thermodynamic and Kinetic Characterization of Membrane Protein Conformational Transitions." In Methods in Molecular Biology. Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1394-8_16.

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Conference papers on the topic "Conformational characterization"

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Kim, Sungho, Angelica M. Palomino, and Nikolas J. Podraza. "Characterization of Adsorbed Polymer Conformational Response Using Spectroscopic Ellipsometry." In GeoCongress 2012. American Society of Civil Engineers, 2012. http://dx.doi.org/10.1061/9780784412121.117.

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Couzon, Cecile, Daniel Banks, James Jonkman, et al. "Characterization of the conformational changes of a tripartite molecular beacon." In Biomedical Optics 2004, edited by Ammasi Periasamy and Peter T. C. So. SPIE, 2004. http://dx.doi.org/10.1117/12.537423.

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Koder, Ronald L., David T. Crouse, Derek J. Kosciolek, et al. "Handheld highly selective plasmonic chem/biosensor using engineered binding proteins for extreme conformational changes." In Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XV, edited by Takuo Tanaka and Din Ping Tsai. SPIE, 2017. http://dx.doi.org/10.1117/12.2273207.

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Ali Khajeh, Jahan, Moussoubaou Atchiba, and Zimei Bu. "Abstract C298: Conformational characterization of tumor suppressor protein Merlin and phosphomimetic Merlin(S518D) mutant in solution." In Abstracts: AACR-NCI-EORTC International Conference: Molecular Targets and Cancer Therapeutics--Oct 19-23, 2013; Boston, MA. American Association for Cancer Research, 2013. http://dx.doi.org/10.1158/1535-7163.targ-13-c298.

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Vo, Elizabeth Donohue, Juan Luengo, Hong Lin, et al. "Abstract LBA007: Discovery and characterization of oncogenic KRAS:RAF1 conformational modulators with in vitro and in vivo MAPK inhibition." In Abstracts: AACR-NCI-EORTC Virtual International Conference on Molecular Targets and Cancer Therapeutics; October 7-10, 2021. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1535-7163.targ-21-lba007.

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Albuquerque, Ana Carolina Ferreira de, José Walkimar de Mesquita Carneiro, and Fernando Martins dos Santos Junior. "Estudo do tautomerismo ceto-enólico da 7-epi-clusianona através de cálculos teóricos de deslocamentos químicos de RMN." In VIII Simpósio de Estrutura Eletrônica e Dinâmica Molecular. Universidade de Brasília, 2020. http://dx.doi.org/10.21826/viiiseedmol202063.

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The properties of natural products, including their biological and pharmacological activities, are directly correlated with their chemical structures. Thus, a correct structural characterization of these compounds is a crucial step to the understanding of their biological activities. However, despite the recent advances in spectroscopic techniques, structural studies of natural products can be challenging. This way, theoretical calculations of Nuclear Magnetic Resonance (NMR) parameters (such as chemical shifts and coupling constants) have proven to be a powerful and low-cost tool for the aid
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Higashiyama, S., I. Ohkubo, H. Ishiguro, and M. Sasaki. "A NEW FUNCTION OF HUMAN KININOGENS: THE AMINO-TERMINAL REGION OF DOMAIN 1 INVOLVES AN EF HAND-LIKE STRACTURE FOR METAL BINDING." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642851.

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Two types of kininogens in mammalian plasma, high molecular weight (HMW) and low molecular weight (LMW) kininogens, are the precursors of kinin. Especially, HMW kininogen circulates in the plasma as a complex with prekallikrein and factor XI, and functions as a cofactor in the initial phase reactions of intrinsic blood coagulation cascade. Recently, it has been found that the kininogens have inhibitory activity toward cysteine proteinases. The heavy chain portion, which is identical for HMW and LMW kininogens, is composed of three domains, domain 1, 2 and 3. Each the domain 2 and 3 has a react
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Zhao, Yang, and Yun-Sheng Chen. "Nanoscopic imaging of molecular conformations in physiological conditions." In Plasmonics: Design, Materials, Fabrication, Characterization, and Applications XVIII, edited by Takuo Tanaka and Din Ping Tsai. SPIE, 2020. http://dx.doi.org/10.1117/12.2568640.

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Globus, Tatiana, Tatyana Khromova, Rebecca Lobo, Dwight Woolard, Nathan Swami, and Erik Fernandez. "THz characterization of lysozyme at different conformations." In Defense and Security, edited by R. Jennifer Hwu, Dwight L. Woolard, and Mark J. Rosker. SPIE, 2005. http://dx.doi.org/10.1117/12.603581.

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Mosca, A., S. Viganò D'Angelo, and A. D'Angelo. "ACTIVATION OF PROTEIN C INDUCES CHANGES IN ITS INTRINSIC FLUORESCENCE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644302.

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Upon activation with either thrombin (T) or thrombin-thrombo modulin complex (T-TM), the zymogen protein C (PC) is transformed into a serine-protease, activated protein C(APC), by release of a small activation peptide. The rate of PC activation changes dramatically with T or with T-TM as a function of the Ca++ concentration in the activation medium, suggesting a configurational change of the zymogen in the presence of Ca++ . It has been shown that Ca++ binding to one single high-affinity binding site of gla-domainless PC is accompanied by a significant decrease of the intrinsic fluo rescence e
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Reports on the topic "Conformational characterization"

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Ashani, Y., and I. Silman. Characterization by NMR and Fluorescence Spectroscopy of Differences in the Conformation of Non-aged and Aged Organophosphoryl Conjugates of AChE. Defense Technical Information Center, 1985. http://dx.doi.org/10.21236/ada202381.

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Ashani, Y., and I. Silman. Characterization by NMR and Fluorescence Spectroscopy of Differences in the Conformation of Non-Aged and Aged Organophosphoryl Conjugates of AChE. Defense Technical Information Center, 1990. http://dx.doi.org/10.21236/ada238708.

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Sadot, Einat, Christopher Staiger, and Mohamad Abu-Abied. Studies of Novel Cytoskeletal Regulatory Proteins that are Involved in Abiotic Stress Signaling. United States Department of Agriculture, 2011. http://dx.doi.org/10.32747/2011.7592652.bard.

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In the original proposal we planned to focus on two proteins related to the actin cytoskeleton: TCH2, a touch-induced calmodulin-like protein which was found by us to interact with the IQ domain of myosin VIII, ATM1; and ERD10, a dehydrin which was found to associate with actin filaments. As reported previously, no other dehydrins were found to interact with actin filaments. In addition so far we were unsuccessful in confirming the interaction of TCH2 with myosin VIII using other methods. In addition, no other myosin light chain candidates were found in a yeast two hybrid survey. Nevertheless
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