Journal articles on the topic 'Complex-component element'

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1

Xie, Li Yang, Wen Xue Qian, and Ning Xiang Wu. "Reliability Model for Complex Mechanical Component Design." Applied Mechanics and Materials 365-366 (August 2013): 28–31. http://dx.doi.org/10.4028/www.scientific.net/amm.365-366.28.

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Taking into account the uncertainty in material property and component quality, a complex mechanical component such as a gear should be treated as a series system instead of a component when evaluating its reliability, since there exist many sites of equal likelihood to fail. Besides, conventional system reliability model is not applicable to such a system because of the statistical dependence among the failures of the every element (damage site). The present paper presents a model to estimate complex mechanical component reliability by incorporating order statistic of element strength into load-strength interference analysis, which can deal with multiple failure mechanisms, reflect statistical dependence among element failure events and that among different failure modes.
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2

Yin, Xiao Wei, Wen Xue Qian, and Li Yang Xie. "Structural Analysis of Complex Aluminum Alloy Structural Component." Applied Mechanics and Materials 602-605 (August 2014): 49–52. http://dx.doi.org/10.4028/www.scientific.net/amm.602-605.49.

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Aluminum alloy structures are widely used in engineering practice. The advantage of aluminum alloy is light weight and corrosion resistance. For different application fields, the structures of aluminum alloy components are very different. They are typically lighter for the same strength and provide better heat conduction. As we know that do FEA (finite element analysis) is necessary before and after the alloy structures have been made. In this paper, a detail analysis was done with FEM (finite element method), and the stress distribution of alloy structures was obtained. Also the FEA results show that the maximum stress is much less than the yield stress and the stress concentration of the round is need to notice.
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3

Xie, Li Yang, and Shao Ze Yan. "A Unified Reliability Modeling Approach for Mechanical System and Complex Component." Advanced Materials Research 308-310 (August 2011): 1416–19. http://dx.doi.org/10.4028/www.scientific.net/amr.308-310.1416.

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With the scenario of reliability estimation, a geometrically complex mechanical/structural component with multiple damage sites should be treated as a system, since there are many links (damage sites) of similar failure probabilities on any of such a component and the failures of the individual damage sites are not perfectly dependent of each other. Conventional system reliability model is not applicable to such a system because of the statistical dependence among the element (damage site) failures. To estimate the reliability of a mechanical system (or a complex component) in which element (damage site) failure dependence plays an important role, a model capable of reflecting the effect of element failure dependence is necessary. The present paper develops models which can deal with multiple damage sites and multiple failure mechanisms, reflect the dependence among element failure events and that among different failure modes. Such models are applicable to both typical mechanical systems and various components.
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4

Bergin, M., T. A. Myles, A. Radić, C. J. Hatchwell, S. M. Lambrick, D. J. Ward, S. D. Eder, A. Fahy, M. Barr, and P. C. Dastoor. "Complex optical elements for scanning helium microscopy through 3D printing." Journal of Physics D: Applied Physics 55, no. 9 (November 26, 2021): 095305. http://dx.doi.org/10.1088/1361-6463/ac3a3e.

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Abstract Developing the next generation of scanning helium microscopes requires the fabrication of optical elements with complex internal geometries. We show that resin stereolithography (SLA) 3D printing produces low-cost components with the requisite convoluted structures whilst achieving the required vacuum properties, even without in situ baking. As a case study, a redesigned pinhole plate optical element of an existing scanning helium microscope was fabricated using SLA 3D printing. In comparison to the original machined component, the new optical element minimised the key sources of background signal, in particular multiple scattering and the secondary effusive beam.
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5

Weis, L., and D. Reinberg. "Accurate positioning of RNA polymerase II on a natural TATA-less promoter is independent of TATA-binding-protein-associated factors and initiator-binding proteins." Molecular and Cellular Biology 17, no. 6 (June 1997): 2973–84. http://dx.doi.org/10.1128/mcb.17.6.2973.

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Two promoter elements, the TATA element and initiator (Inr), are capable of directing specific transcription initiation of protein-encoding genes by RNA polymerase II (RNAPII). Although binding to the TATA element by the TATA-binding protein (TBP) has been shown to be the initial recognition step in transcription complex formation in vitro, the mechanism through which the basal machinery assembles into a functional complex on TATA-less promoters is controversial. Evidence supporting numerous models of Inr-mediated transcription complex formation exists, including the nucleation of a complex by Inr-binding proteins, a component of the TFIID complex, or a specific upstream activator common to many TATA-less promoters, Sp1. Using various techniques, we have undertaken a systematic analysis of the natural TATA-less human DNA polymerase beta (beta-pol) gene promoter. Although the beta-pol promoter contains upstream Sp1 elements and a functional Inr that binds YY1, neither of these factors is essential for Inr-mediated transcription complex formation. A complex containing TBP, TFIIB, TFIIF, and RNAPII (DBPolF complex) is capable of forming on the promoter in an Inr-dependent manner. A single point mutation within the Inr that affects DBPolF complex formation diminishes beta-pol transcriptional activity.
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6

Kumaresan, Srirangam, Narayan Yoganandan, and Frank A. Pintar. "Posterior Complex Contribution on Compression and Distraction Cervical Spine Behavior: A Finite Element Model." Journal of Musculoskeletal Research 02, no. 03 (September 1998): 257–65. http://dx.doi.org/10.1142/s0218957798000251.

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The relative contributions of the posterior elements on the human cervical spine behavior under axial compression and distraction were determined using the finite element modeling approach. This was accomplished by considering two structures: First, the intervertebral joint structure representing the anterior and posterior columns, including the C4–C6 vertebrae and the neighboring discs with the entire ligamentous complex; and second, the disc joint structure representing only the anterior column (vertebral bodies, discs and longitudinal ligaments). The anterior column sustained a higher share of the load than the posterior column under distraction. However, compression was shared equally among the anterior and the two posterior load bearing columns. The component internal stresses increased with posterior element removal. Consequently, inactivation or compromise in the posterior elements introduces additional internal loads or stresses in the anterior components which may accentuate degenerative changes or injure the cervical column. Thus, posterior complex integrity is critical for the load-carrying capacity of the cervical spine.
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7

Majidi, Omid, Mohammad Jahazi, and Nicolas Bombardier. "Finite Element Simulation of High-Speed Blow Forming of an Automotive Component." Metals 8, no. 11 (November 3, 2018): 901. http://dx.doi.org/10.3390/met8110901.

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High-speed blow forming (HSBF) is a new technology for producing components with complex geometries made of high strength aluminum alloy sheets. HSBF is considered a hybrid-superplastic forming method, which combines crash forming and gas blow forming. Due to its novelty, optimization of the deformation process parameters is essential. In this study, using the finite element (FE) code ABAQUS, thinning of an aluminum component produced by HSBF under different strain rates was investigated. The impact of element size, variation of friction coefficient, and material constitutive model on thinning predictions were determined and quantified. The performance of the FE simulations was validated through forming of industrial size parts with a complex geometry for the three investigated strain rates. The results indicated that the predictions are sensitive to the element size and the coefficient of friction. Remarkably, compared to a conventional power law model, the variable m-value viscoplastic (VmV) model could precisely predict the thickness variation of the industrial size component.
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8

Rapaport, Doron, and Walter Neupert. "Biogenesis of Tom40, Core Component of the Tom Complex of Mitochondria." Journal of Cell Biology 146, no. 2 (July 26, 1999): 321–32. http://dx.doi.org/10.1083/jcb.146.2.321.

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Tom40 is an essential component of the preprotein translocase of the mitochondrial outer membrane (TOM complex) in which it constitutes the core element of the protein conducting pore. We have investigated the biogenesis of Tom40. Tom40 is inserted into the outer membrane by the TOM complex. Initially, Tom40 is bound as a monomer at the mitochondrial surface. The import receptor Tom20 is involved in this initial step; it stimulates both binding and efficient insertion of the Tom40 precursor. This step is followed by the formation of a further intermediate at which the Tom40 precursor is partially inserted into the outer membrane. Finally, Tom40 is integrated into preexisting TOM complexes. Efficient import appears to require the Tom40 precursor to be in a partially folded conformation. Neither the NH2 nor the COOH termini are necessary to target Tom40 to the outer membrane. However, the NH2-terminal segment is required for Tom40 to become assembled into the TOM complex. A model for the biogenesis of Tom40 is presented.
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9

Company, M., and B. Errede. "A Ty1 cell-type-specific regulatory sequence is a recognition element for a constitutive binding factor." Molecular and Cellular Biology 8, no. 12 (December 1988): 5299–309. http://dx.doi.org/10.1128/mcb.8.12.5299.

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Ty transposable-element insertion mutations of Saccharomyces cerevisiae can cause cell-type-dependent activation of adjacent-gene expression. Several cis-acting regulatory regions within Ty1 are responsible for the effect of Ty1 on adjacent-gene expression. One of these is the block II sequence that was defined by its homology to mammalian enhancers and to the yeast a1-alpha 2 control site. Tandem copies of a 57-base-pair region encompassing block II caused an additive increase in expression of the CYC7 reporter gene in the absence of other Ty1 sequences. The activation of gene expression by the multiple repeats was abolished in a/alpha diploid cells. A specific complex between a constitutive factor in whole-cell extracts and the DNA regulatory element was observed. The protein-binding site for the constitutive factor coincided with the block II element. Base-pair substitutions within the binding site abolished the ability of the block II element to function as a component of the Ty1 activator and to form the factor-DNA complex. The correlation between complex formation and reporter gene expression indicates that factor binding to the cis-acting element is essential for this element to function as a component of the Ty1 activator.
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10

Company, M., and B. Errede. "A Ty1 cell-type-specific regulatory sequence is a recognition element for a constitutive binding factor." Molecular and Cellular Biology 8, no. 12 (December 1988): 5299–309. http://dx.doi.org/10.1128/mcb.8.12.5299-5309.1988.

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Ty transposable-element insertion mutations of Saccharomyces cerevisiae can cause cell-type-dependent activation of adjacent-gene expression. Several cis-acting regulatory regions within Ty1 are responsible for the effect of Ty1 on adjacent-gene expression. One of these is the block II sequence that was defined by its homology to mammalian enhancers and to the yeast a1-alpha 2 control site. Tandem copies of a 57-base-pair region encompassing block II caused an additive increase in expression of the CYC7 reporter gene in the absence of other Ty1 sequences. The activation of gene expression by the multiple repeats was abolished in a/alpha diploid cells. A specific complex between a constitutive factor in whole-cell extracts and the DNA regulatory element was observed. The protein-binding site for the constitutive factor coincided with the block II element. Base-pair substitutions within the binding site abolished the ability of the block II element to function as a component of the Ty1 activator and to form the factor-DNA complex. The correlation between complex formation and reporter gene expression indicates that factor binding to the cis-acting element is essential for this element to function as a component of the Ty1 activator.
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11

Albers, Bettina, Stavros A. Savidis, H. Ercan Taşan, Otto von Estorff, and Malte Gehlken. "BEM and FEM results of displacements in a poroelastic column." International Journal of Applied Mathematics and Computer Science 22, no. 4 (December 28, 2012): 883–96. http://dx.doi.org/10.2478/v10006-012-0065-y.

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The dynamical investigation of two-component poroelastic media is important for practical applications. Analytic solution methods are often not available since they are too complicated for the complex governing sets of equations. For this reason, often some existing numerical methods are used. In this work results obtained with the finite element method are opposed to those obtained by Schanz using the boundary element method. Not only the influence of the number of elements and time steps on the simple example of a poroelastic column but also the impact of different values of the permeability coefficient is investigated.
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12

Zhang, Xue Ling, Ya Hui Hu, and Shu Feng Chai. "Modeling Method for Complex Structure System in Finite Element Simulating Analysis." Applied Mechanics and Materials 130-134 (October 2011): 195–99. http://dx.doi.org/10.4028/www.scientific.net/amm.130-134.195.

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How to deal with the model is important in analyzing structure system with finite element method, because the rationality of model has direct relationship with veracity of simulation result. In this paper some simplify modeling methods are expatiated. And an approach is supposed that combine simplifying complex model and rigid-treating sub-component which make model more close to reality. In final, an instance of multi-type-element system structure is computed to prove the correctness of the method.
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13

Zhang, Yu Fu, Hui Xia Guo, Jun Chen Li, Gui Rong Yang, Ying Ma, Wen Min Song, Jie Sheng, and Yuan Hao. "Simulation Research of a Type of Pressure Vessel under Complex Loading Part 1 Component Load of the Numerical Analysis." Advanced Materials Research 756-759 (September 2013): 4656–61. http://dx.doi.org/10.4028/www.scientific.net/amr.756-759.4656.

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Macroscopic mechanical response is one of the key factors in designing pressure vessel. A geometric modeling of pressure vessel was established and the mesh of this modeling then generated by using the finite element simulating methods in software ABAQUS. Loading and boundary conditions of dead weight, hydraulic and uniform internal pressure which often suffered pressure vessel were set and calculated by the finite element method. Stress/strain response of pressure vessel in all kinds of alone loading ways were obtained. The results of finite element simulating were in accordance with those of theoretical calculation which provided useful data for research on mechanical response of pressure vessel under complex loading conditions.
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14

Zhelyazov, Todor. "Structural Materials: Identification of the Constitutive Models and Assessment of the Material Response in Structural Elements Strengthened with Externally-Bonded Composite Material." Materials 13, no. 6 (March 11, 2020): 1272. http://dx.doi.org/10.3390/ma13061272.

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This article investigates the material behavior within multiple-component systems. Specifically, a structural concrete element strengthened to flexure with externally-bonded fiber-reinforced polymer (FRP) material is considered. Enhancements of mechanical performances of the composite structural element resulting from synergies in the framework of the multiple-component system are studied. The research work comprises the determination of the constitutive relations for the materials considered separately as well as the investigation of materials’ response within a complex system such as the composite structural element. The definition of the material models involves a calibration of the model constants based on characterization tests. The constitutive relations are integrated into the finite element model to study the material behavior within the multiple-component system. Results obtained by finite element analysis are compared with experimental results from the literature. The finite element analysis provides valuable information about the evolution of some internal variables, such as mechanical damage accumulation. The material synergies find expression in the load-carrying capacity enhancement and the delay in the damage accumulation in concrete.
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15

Sita, Dan Dragos, Ligia Brezeanu, Cristina Bica, Dana Manuc, Edwin Sever Bechir, Marius Hack, and Mariana Pacurar. "Stress Variation in the Orthodontic Tipping Phenomenon Analysis through the finite element method." Revista de Chimie 69, no. 8 (September 15, 2018): 1992–95. http://dx.doi.org/10.37358/rc.18.8.6460.

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The purpose of the study is to assess through a FEM (Finite Element Method analysis), the behavior of a complex structure (enamel-tooth-alveolar bone-periodontal ligament-pulp), subjected to an external load through an orthodontic bracket-with forces of various intensities and to determine its influence on the entire structure.It is necessary to analyze the way all elements of the structure take over the external action given by the action of an orthodontic appliance through the brackets and the influence on the inner component -the pulp-inside of which there are the nerve endings.
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16

Freidman, Oksana, and Irina Smirnova. "The Corruption Component in Transport and Logistics Services: a Causal Complex." Russian Journal of Criminology 12, no. 5 (November 8, 2018): 669–77. http://dx.doi.org/10.17150/2500-4255.2018.12(5).669-677.

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The authors analyze the problems of contemporary criminal policy and transport policy regarding the prevention of corruption and the effectiveness of combating it. They highlight the development of transport and logistics services. The authors use statistical data reflecting specific features of contemporary transport and anti-corruption policies to show that it is only possible to solve the urgent theoretical and practical problem of counteracting corruption in a specific business sphere if the used methodological instruments are poly-functional in nature. The key causes and conditions promoting corruption in this sector are such factors as multiple subject contacts, a large number of sanitary, labor and safety regulations, intense competition among transport companies due to a high concentration of companies on the market of transport and logistics services, their transition to the outsourcing sector, the development of ecologistics, etc. That is why corruption prevention should be carried out at the general social and the special criminological level. To prevent corruption in transport and logistics services at these levels the authors recommend, on the one hand, to modernize transport policy by widening the use of information technologies, organizing combined transportation, etc. On the other hand, it is necessary to develop the mechanisms of preventing and regulating conflicts of interests while stressing the prohibition element and to synchronize the transport and the criminal policy of the state that concerns combating corruption in this sector.
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17

Karpel, M., B. Moulin, and V. Feldgun. "Component Mode Synthesis of a Vehicle System Model Using the Fictitious Mass Method." Journal of Vibration and Acoustics 129, no. 1 (January 8, 2006): 73–83. http://dx.doi.org/10.1115/1.2202156.

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A new procedure for dynamic analysis of complex structures, based on the fictitious-mass component mode synthesis method, is presented. Normal modes of separate components are calculated by finite-element analysis with the interface coordinates loaded with fictitious masses that generate local boundary deformations in the low-frequency modes. The original fictitious-mass method is extended to include three types of component interconnections: displacement constraints, connection elements, and structural links. The connection elements allow the introduction of springs and dampers between the interface points without adding structural degrees of freedom. The structural links facilitate the inclusion the discrete finite-element representation of typically small components in the coupling equations. This allows a convenient treatment of loose elements and the introduction of nonlinear effects and parametric studies in subsequent analyses. The new procedure is demonstrated with the structural model of a typical vehicle with four major substructures and a relatively large number of interface coordinates. High accuracy is obtained in calculating the natural frequencies and modes of the assembled structure and the separate components with the fictitious masses removed. Dynamic response analysis of the vehicle travelling over a rough road, performed by modal coupling, is in excellent agreement with that performed for the full model.
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18

La Vista-Picard, N., P. D. Hobbs, M. Pfahl, M. I. Dawson, and M. Pfahl. "The receptor-DNA complex determines the retinoid response: a mechanism for the diversification of the ligand signal." Molecular and Cellular Biology 16, no. 8 (August 1996): 4137–46. http://dx.doi.org/10.1128/mcb.16.8.4137.

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To obtain insights into the principles governing the complex biological responses to retinoids, we have analyzed the ligand sensitivities of various retinoid receptor-DNA complexes. We find that different retinoid receptor heterodimers show distinct activation patterns with various response elements while a given heterodimer can be activated at different retinoic acid concentrations on different response elements. In vitro binding experiments suggest that the same retinoic acid receptor-retinoid X receptor (RAR-RXR) heterodimer can have different ligand affinities, depending on the response element it is bound to. The differential responses of a particular receptor heterodimer with various retinoic acid responsive elements can be enhanced through the use of conformationally restricted retinoids. RAR- and RXR-selective retinoids can also synergistically activate the receptor heterodimers, indicating that both partners in the heterodimer can contribute to ligand-induced transcriptional activation. However, the relative influence of the RAR or RXR partner is specific for each response element. Together, our data demonstrate that it is the receptor-DNA complex and not the receptor alone that determines the ligand response. This flexibility allows for a highly pleiotropic retinoid response. Furthermore, conformationally restricted retinoids can accentuate the differential responses and exhibit a certain degree of gene selectivity by differentially activating the RAR or RXR component in the context of a given response element.
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19

Ghouilem, Kamel, Rachid Mehaddene, and Mohammed Kadri. "Contact Friction Simulating between Two Rock Bodies Using ANSYS." International Journal of Engineering Research in Africa 29 (March 2017): 1–9. http://dx.doi.org/10.4028/www.scientific.net/jera.29.1.

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The ANSYS® Finite Element Method (FEM) program offers a variety of elements designed to treat cases of changing mechanical contact between the parts of an assembly or between different faces of a single part. These elements range from simple, limited idealizations to complex and sophisticated, general purpose algorithms. Contact problems are highly nonlinear and require significant computer resources to solve. Recently, analysts and designers have begun to use numerical simulation alone as an acceptable mean of validation employing numerical Finite Element Method (FEM). Contact problems fall into two general classes: rigid-to-flexible and flexible-to-flexible. In general, any time a soft material comes in contact with a hard material, the problem may be assumed to be rigid-to-flexible. The other class, flexible-to-flexible, is the more common type. To model a contact problem, you first need to identify the parts to be analyzed for their possible interaction. If one of the interactions is at a point, the corresponding component of your model is a node. If one of the interactions is at a surface, the corresponding component of your model is an element. The finite element model recognizes possible contact pairs by the presence of specific contact elements. These contact elements are overlaid on the parts of the model that are being analyzed for interaction. This paper present a simulation contact friction between Two Rock bodies loaded under two types of load condition: Axial pressure Load “σ” and Tangential Load “τ”. ANSYS® software has been used to perform the numerical calculation in this paper.
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20

Peng, Jian, Chang Yu Zhou, Ji Lin Xue, Xiao Hua He, and Qiao Dai. "Finite Element Simulation of Limit Load of Components at High Temperature." Materials Science Forum 704-705 (December 2011): 1291–97. http://dx.doi.org/10.4028/www.scientific.net/msf.704-705.1291.

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The creep of materials makes it difficulty to determine the limit load of component at high temperature. In this paper, limit load was obtained by finite element simulation according to isochronous stress versus cumulative strain data and creep failure criterion at high temperature. Firstly, isochronous stress versus cumulative strain data of P91 steel was generated. In finite element analysis code ABAQUS, isochronous stress versus cumulative strain data was replaced by equivalent elastic-plastic constitutive relation. Then, sustained load versus cumulative strain curves at high temperature during service was obtained after finite element simulation. At last, limit load at high temperature during given working hours was determined based on the restriction of total strain at key point of specific component. The restriction of total strain which could also be regarded as long-term fracture strain was discussed in this paper. Finite element simulation of limit load of component at high temperature is simple and reasonable. Limit load of complex component at high temperature during given working hours can be obtained by this method. Using this method, limit loads of a pipe with local wall thinning defect and a branch junction at high temperature during given working hours were obtained as examples.
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21

Adams, Allen D., Donna M. Choate, and Mary Ann Thompson. "NF1-L Is the DNA-binding Component of the Protein Complex at the Peripherin Negative Regulatory Element." Journal of Biological Chemistry 270, no. 12 (March 24, 1995): 6975–83. http://dx.doi.org/10.1074/jbc.270.12.6975.

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Adamas, Allen D., Donna M. Choate, and Mary Ann Thompson. "NF1-L is the DNA-binding component of the protein complex at the peripherin negative regulatory element." Journal of Biological Chemistry 270, no. 33 (August 18, 1995): 19668.1–19668. http://dx.doi.org/10.1074/jbc.270.33.19668.

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23

Tsarev, Yuri. "Monitoring of priorii information at complex product failure of single use in the process of its creation." E3S Web of Conferences 135 (2019): 01062. http://dx.doi.org/10.1051/e3sconf/201913501062.

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A variant of the parametric representation of a single use complex product in the process of its creation during testing is described, when structural failures of the product or its component parts according to one or more parameters specified in the technical specification, requires the implementation of modifications is required, leading to changes in general set and repetition trials, however, it is possible to reduce the volume of tests by taking into account a priorii information. This is especially true, while ensuring the reliability of complex space products of single use, the components of which pass the first stages of ground tests, and then as part of complex space products the stages of flight tests. The structural failure of any component of a space product during ground testing must not have the effect of occurring during flight tests as if it had been part of the product. Meanwhile, the completion of the component element of a singleuse product, the structural failure of which occurred at the stage of flight tests in the product, and the evaluation of its effectiveness, can be carried out at the stage of ground tests, in the case of a complex product consideration in parametric form. It can significantly reduce the cost of flight testing of a complex product, in the case of the product composite element effective refinement at the stage of ground testing, without a significant (temporary) reduction in the overall reliability of a single use complex product.
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24

Chen, Yan, Song Wei Wang, Hong Wu Song, and Shi Hong Zhang. "Forging Process Design and Simulation Optimization of a Complex-Shaped Aluminium Alloy Component." Materials Science Forum 941 (December 2018): 784–89. http://dx.doi.org/10.4028/www.scientific.net/msf.941.784.

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In order to meet the requirements of lightweight and replace steel with the aluminum for a component on the high speed rail, the forging process of a complex-shaped aluminum alloy component was researched and the parameters were optimized with the DEFORM-3D finite element simulation technology. The qualified products were finally obtained instead of the original steel castings by reducing weight of 65%. It is noted that the parts with complicated shape and non-symmetry, metal flow uneven during forging process that lead to incomplete forming, higher forging pressure problems. In this paper, such problems were analyzed couple with numerical simulation method based on a certain forming pressure. Moreover, the model and slot was reasonably designed. In addition, the size of blank was constantly optimized to change the metal flows direction and cavity filling mode. Finally, the forgings with good surface quality and mechanical properties were obtained by production test, and can be used as reference for this kind of forging components.
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25

Bychkova, Ya V., D. P. Starodymova, K. V. Shaikhutdinova, D. R. Diagileva, M. A. Semernin, E. M. Dekunova, I. Yu Nikolayeva, and A. Yu Bychkov. "Particularity of chemical preparation of bottom sediment samples for multi-element ICP-MS analysis." Moscow University Bulletin. Series 4. Geology, no. 4 (August 28, 2020): 45–54. http://dx.doi.org/10.33623/0579-9406-2020-4-45-54.

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Various techniques have been tested (multi-acid microwave decomposition and sintering with soda) for preparing samples for the analysis of ICP-MS for samples with a complex matrix including an organic component for the subsequent determination of the microelement composition. For research, we used samples of bottom sediments: terrigenous clay (SDO-1), volcanic-terrigenous silt (SDO-2), calcareous silt (SDO-3), ferromanganese nodules (SDO-5), Baikal silt (BIL-1) and complex sediment sample of Baikal (BIL-2). The most effective sample preparation methods for the studied rock type are proposed.
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26

MURAO, SATOSHI, and SOEY H. SIE. "PIXE AS AN ESSENTIAL TOOL FOR RARE METAL BENEFICIATION AND EXPLORATION." International Journal of PIXE 05, no. 02n03 (January 1995): 97–103. http://dx.doi.org/10.1142/s0129083595000137.

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We have examined tin-polymetallic ore, a complex mixture of cassiterite (SnO2) and sulfides, by micro-PIXE. Tin-polymetallic ore is one of the major sources of technologically important “rare metals”, especially of indium and bismuth, usually as trace elements. In addition to such rare metals, silver is another important trace component in the ore. But the trace elemental distribution of tin-polymetallic deposit has not well been described due to the small size of constituent minerals, complex ore texture, and lack of analytical method to detect trace elements in a small area. PIXE with a proton microbeam could be an effective tool to solve this problem by delineating the distribution of these trace elements among carrier minerals with the required sensitivity. Thus we have applied PIXE with the CSIRO’s proton microprobe to a tin-polymetallic ore from Canada. The result showed that micro-PIXE is an essential tool to study trace element distribution in such a complex ore.
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27

Zach, Theodor-Florian, and Mircea-Cristian Dudescu. "The Topological Optimization and the Design for Additive Manufacturing of a Steering Knuckle for Formula SAE Electric Vehicle." MATEC Web of Conferences 343 (2021): 04011. http://dx.doi.org/10.1051/matecconf/202134304011.

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Formula Student represents the main motorsport activity for future professionals during their academic preparation. There is intensive migration from the prototypes with an internal combustion engine to electrical vehicles. The challenge of electric vehicles is the weight of the batteries which has to be compensated. The Topological Optimization process represents a method of removing volume and mass of the component (elements of mesh) with the aid of the discretization allowed by the finite element method (FEM) until a mass or stress constraint is attained. The results come in a complex shape, the material being kept only in the stressstrain directions. The manufacturing process is usually as complex, being employed high complexity technologies such as Selective Laser Melting (SLM). Improved strength-to-weight ratio materials are to be considered to obtain the most performant design of a specific component. The present paper presents the topological optimization process of the steering knuckle for the Formula Student electric vehicle of ART-TU Team of Technical University of Cluj-Napoca. It means that the part is simulated and optimized through Ansys Static Structural and there is done post-processing of the component along with Data Validation. The conclusions consist of the viability of the Topological Optimization process when designing complex components for performance automotive.
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28

Pluta, Zdzisław, and Tadeusz Hryniewicz. "Kinetics of Flexibly Fixed Operating Element of a Rotating Device." International Letters of Chemistry, Physics and Astronomy 8 (September 2013): 67–84. http://dx.doi.org/10.18052/www.scipress.com/ilcpa.8.67.

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The work covers the adequate kinetics of the flexibly fixed working element of a rotating device. First the literature background of the problem has been presented, turning the attention on such systems where the phenomenon of centrifugal displacement of a body due to the rotating of the system occurs. Then the general characteristics of the complex motion of working element has been described. The core of the work is the description of the variable component motions, with the starting point of the description being the source differential equation, presenting this type of dependence of the path length on time. The accelerated angular motion and retarded radial motion have been separated, describing these component motions as the function of time. Based on these detailed descriptions, the trajectory equation of working element, the derivatives and further kinetic magnitudes, have been derived. In the conclusion, the cognitive and practical qualities of the presented solutions of the title problem, have been accented.
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29

Stargell, L. A., and K. Struhl. "A new class of activation-defective TATA-binding protein mutants: evidence for two steps of transcriptional activation in vivo." Molecular and Cellular Biology 16, no. 8 (August 1996): 4456–64. http://dx.doi.org/10.1128/mcb.16.8.4456.

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Using a genetic screen, we isolated four TATA-binding protein (TBP) mutants that are specifically defective in vivo for the response to acidic activators. In contrast to previously described activation-defective TBP mutants, these TBP derivatives are not specifically defective for interactions with TATA elements or TFIIA. Three of these derivatives interact normally with a TATA element, TFIIA, TFIIB, or an acidic activation domain; presumably, they affect another protein-protein interaction important for transcriptional activation. The remaining derivative (with F-237 replaced by D) binds a TATA element with wild-type affinity, but the TBP-TATA complex has an altered electrophoretic mobility and interacts poorly with TFIIA and TFIIB; this suggests that the conformation of the TBP-TATA element complex plays a role in transcriptional activation. To determine the step at which the TBP derivatives were unable to activate transcription, we utilized an artificial recruitment assay in which TBP is targeted to the promoter via fusion to the LexA DNA-binding domain. Consistent with previous evidence that acidic activators can increase recruitment of TBP to the promoter in vivo, the activation defect of some of these TBP derivatives can be corrected by artificial recruitment. In contrast, the activation defect of the other TBP derivatives is not bypassed by artificial recruitment. Thus, these TBP mutants define two steps in the process of transcriptional stimulation by acidic activators: efficient recruitment to the TATA element and a postrecruitment interaction with a component(s) of the initiation complex.
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30

Ayyagari, Aditya, Vahid Hasannaeimi, Harpreet Grewal, Harpreet Arora, and Sundeep Mukherjee. "Corrosion, Erosion and Wear Behavior of Complex Concentrated Alloys: A Review." Metals 8, no. 8 (August 3, 2018): 603. http://dx.doi.org/10.3390/met8080603.

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There has been tremendous interest in recent years in a new class of multi-component metallic alloys that are referred to as high entropy alloys, or more generally, as complex concentrated alloys. These multi-principal element alloys represent a new paradigm in structural material design, where numerous desirable attributes are achieved simultaneously from multiple elements in equimolar (or near equimolar) proportions. While there are several review articles on alloy development, microstructure, mechanical behavior, and other bulk properties of these alloys, then there is a pressing need for an overview that is focused on their surface properties and surface degradation mechanisms. In this paper, we present a comprehensive view on corrosion, erosion and wear behavior of complex concentrated alloys. The effect of alloying elements, microstructure, and processing methods on the surface degradation behavior are analyzed and discussed in detail. We identify critical knowledge gaps in individual reports and highlight the underlying mechanisms and synergy between the different degradation routes.
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31

Wu, Daniel Y., Anton Krumm, and William H. Schubach. "Promoter-Specific Targeting of Human SWI-SNF Complex by Epstein-Barr Virus Nuclear Protein 2." Journal of Virology 74, no. 19 (October 1, 2000): 8893–903. http://dx.doi.org/10.1128/jvi.74.19.8893-8903.2000.

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ABSTRACT The multiprotein human SWI-SNF (hSWI-SNF) complex is a chromatin-remodeling machine that facilitates transcription by overcoming chromatin-mediated gene repression. We had previously shown that hSNF5/INI1, an intrinsic, consistent component of the hSWI/SNF complex, is associated with Epstein-Barr nuclear antigen 2 (EBNA2) and have proposed that EBNA2 directs this complex to key EBNA2-responsive viral and cellular genes. Using chromatin immunoprecipitation and quantitative PCR, we show that antibodies directed against components of the hSWI-SNF complex preferentially precipitate chromatin-associated DNA that contains a targeted EBNA2-responsive element in the context of both episomal and cellular chromatin. This enrichment does not occur in EBNA2-negative cells or when the EBNA2-responsive element is mutated. The stable association of the hSWI-SNF complex with the EBNA2-responsive promoter can also be disrupted by deletion of the TATA element, suggesting that EBNA2 in itself is insufficient to mediate stable targeting of the hSWI-SNF complex. These results demonstrate that recruitment of the hSWI-SNF complex to selected promoters can occur in vivo through its interaction with site-specific activator proteins and that stable targeting may require the presence of basal transcription factors.
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32

Linhoff, M. W., K. L. Wright, and J. P. Ting. "CCAAT-binding factor NF-Y and RFX are required for in vivo assembly of a nucleoprotein complex that spans 250 base pairs: the invariant chain promoter as a model." Molecular and Cellular Biology 17, no. 8 (August 1997): 4589–96. http://dx.doi.org/10.1128/mcb.17.8.4589.

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The events that lead to promoter accessibility within chromatin are not completely understood. The invariant chain (Ii) promoter was used as a model to determine the contribution of different DNA-binding factors in establishing occupancy of a complex promoter. Gamma interferon induction of the Ii promoter requires the cooperation of multiple cis elements including distal S, X, and Y/CCAAT elements along with proximal GC and Y/CCAAT elements. The heteromeric transcription factor NF-Y binds to both Y/CCAAT elements. Genomic footprinting was used to analyze in vivo protein-DNA contacts for integrated Ii promoters bearing mutations in each element. The results reveal a hierarchy of transcription factor loading with NF-Y binding to the distal Y/CCAAT element being required for establishing protein-DNA interactions over the entire 250 bp analyzed. Mutation of the X box disrupts binding primarily at the adjacent Y/CCAAT element along with a lesser effect on GC box binding. Importantly, this finding is verified with a cell line which lacks a functional X-box-binding factor, RFX, providing physiological validity for the strategy described here. Mutation of both the S element and the GC box results in either no or little effect on transcription factor binding. However, mutation of the proximal Y/CCAAT element disrupts binding to the adjacent GC box and partially reduces binding in the distal S/X/Y domain. The crucial role for NF-Y in establishing promoter occupancy may be related to its histone fold motif, the essential component for assembling nucleosome-like structures.
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33

Nagaki, Kiyotaka, Paul B. Talbert, Cathy Xiaoyan Zhong, R. Kelly Dawe, Steven Henikoff, and Jiming Jiang. "Chromatin Immunoprecipitation Reveals That the 180-bp Satellite Repeat Is the Key Functional DNA Element of Arabidopsis thaliana Centromeres." Genetics 163, no. 3 (March 1, 2003): 1221–25. http://dx.doi.org/10.1093/genetics/163.3.1221.

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Abstract The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
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34

Floyd, P. A., R. E. Holdsworth, and S. A. Steele. "Geochemistry of the Start Complex greenschists: Rhenohercynian MORB?" Geological Magazine 130, no. 3 (May 1993): 345–52. http://dx.doi.org/10.1017/s0016756800020021.

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AbstractThe meta-igneous greenschists of the Start Complex, SouthDevon, are composed of a mineralogically uniform, but texturally variable, actinolite-epidote-albite assemblage with retrogressed variants containing chlorite, muscovite, sphene, carbonate and oxidized opaque minerals. Geochemically they represent a suite of relatively primitive tholeiites, exhibiting mild differentiation, depleted incompatible element abundances, and variable light rare-earth-element-depletion patterns comparable to modern basalts from normal spreading ridge segments (N-MORB). As the Start greenschistsexhibit a number of chemical similarities to the nearby Upper Palaeozoic Lizard ophiolite, and MORB-type clasts within the Rhenohercynian Zone generally, they may also represent local Variscan ocean crust, which floored smalloceanic basins that separated the Old Red Sandstone continent from the Armorican microplate to the south. The Start Complex could thus represent a previously unrecognized oceanic component to the Variscan orogenic belt (Rhenohercynian Zone) of Northwest Europe.
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35

Abimbola, Raji A. "Method of Moment Determination of Current Distribution on Elements of Yagi-Uda Array." Circulation in Computer Science 2, no. 9 (October 20, 2017): 23–29. http://dx.doi.org/10.22632/ccs-2017-252-56.

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Presented in this paper is the numerical solution to the current distributions on two forms of Yagi-Uda antenna designs. One form consists of twelve elements while the other consists of fourteen elements. Employing method of moments technique in which the unknown current is expanded in terms of known expansion function and complex coefficients which are to be determined. It is demonstrated that, when the integral equation that expresses tangential component of an impressed field in terms of induced current on the elements of Yagi-Uda array is reduced into matrix form, the current distribution of interest becomes known. The profiles for the current distributions on elements of those arrays represented in graphical forms reveal that, the currents are symmetrical about the length of the element in each case. It is found that the highest magnitude of the current exists on the driven element. Furthermore, the characteristic profiles of the currents on elements of those arrays exhibit sinusoidal type of waveform and are largely similar when the frequencies of operation are 200MHz and 665MHz, respectively.
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36

Konjuskevic, Marija. "The preposition potential in formation of conjunctional means." Juznoslovenski filolog, no. 70 (2014): 17–33. http://dx.doi.org/10.2298/jfi1470017k.

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In the article the ability of preposition to form conjunction or its equivalent has been considered. The mechanism of denominalization has been shown on the Russian material with the involvement of the author?s observations on prepositions in the Belarusian language - the morphological form transformation in to the subordinate part of complex sentence. The author?s conception is: 1) the syntaxeme is the actant position in the sentence; 2) the syntaxeme consists of the formant (preposition + flexion) and nominative component (substantive); 3) at the level of the complex sentence the same actant position may take the predicative unit (subordinate part of the complex sentence); 4) the formant of the predicative syntaxeme in transforms is the conjunctional combination ?preposition + demonstrative pronoun that + subordinate part conjunction?. The preposition at case syntaxeme transformation in to predicative does not change its function, but it is included in to the conjunctional formant as one of the elements of more complex formant - conjunctional combination. Each element of the conjunctional combination fulfills its function: the preposition still forms the syntaxeme, but with more complex nominative component as predicative unit, the pronoun that is ?flection? of this nominative component (subordinate part), and the conjunction acts as the explicator, providing grammatical connection of the nominative component of syntaxeme with the ?flexion? and the preposition. It is noted that the mechanism and the results of denominalization depend on the syntaxemes repertoire and character and on the preposition meaning: the same preposition, with different meaning has different conjunctional potential. In to the mechanism of denominalization are involved categorematic names (classifiers), which initially are used as structural vocabulary for forming secondary prepositions, and together with them, for forming conjunctional combinations. The methods of transformation of prepositional-nominal syntaxeme in to conjunctional-predicative reveal more conjunctional means than there are in the existing grammatical and lexicographical descriptions.
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37

Tsui, Chi Pong, Chak Yin Tang, Chi Loong Chow, S. C. Hui, and Y. L. Hong. "Active Finite Element Method for Simulating the Contraction Behavior of a Muscle-Tendon Complex." Advanced Materials Research 9 (September 2005): 9–14. http://dx.doi.org/10.4028/www.scientific.net/amr.9.9.

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A three-dimensional finite element analysis was conducted to simulate the effects of the varying material parameters on the contraction behaviors of a muscle-tendon complex using an active finite element method. The material behavior of the skeletal muscle was assumed to be orthotropic and the muscle model consists of two parts: the active and the passive parts. An active finite element method was then used for accommodating both the active and passive behaviors of the muscle into the muscle model. In this active-passive muscle model, the active component is governed by an activation level, a time period, a muscle sensitivity parameter and a strain rate. The material property of the passive component was assumed to be viscoelastic and the tendon is assumed to be linear elastic. The effects of activation amplitude and viscoelastic material parameters on the active, passive and total force-length relationship of the cat muscle under isometric contraction were predicted. The predicted results were found to be close to the experimental data reported in the available literature. Hence, the active-passive muscle model was extended to simulate the stress distribution of the cat muscle subject to shortening contraction and different activation amplitude. By varying the magnitude of the material parameters, different muscle behaviors could be generated. The proposed active finite element method lays a good foundation for simulation of human musculoskeletal motion.
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38

Yamada, Tomonori, Noriyuki Kushida, Fumimasa Araya, Akemi Nishida, and Norihiro Nakajima. "Component-Wise Meshing Approach and Evaluation of Bonding Strategy on the Interface of Components for Assembled Finite Element Analysis of Structures." Key Engineering Materials 452-453 (November 2010): 701–4. http://dx.doi.org/10.4028/www.scientific.net/kem.452-453.701.

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The finite elements are extensively utilized to solve various problems in engineering fields with the growth of computing technologies. However, there is a lack of methodology for analyses of huge assembled structures. The mechanics on the interface of each components, for instance, contact, bolt joint and welding in assembly is a key issue for important huge structure such as nuclear power plants. On the other hand, it is well known that as finite element models become large and complex, construction of detailed mesh becomes a bottleneck in the CAE procedures. To solve these problems, the authors would like to introduce component-wise meshing approach and bonding strategy on the interface of components. In order to assemble component-wise meshes, the penalty method is introduced not only to constrain the displacements, but also to introduce classical spring connection on the joint interface, although penalty method is claimed that it is not suitable for iterative solver. In this paper, the convergence performance of an iterative solver with penalty method is investigated and the detailed component-wise distributed computation scheme is described with numerical examples.
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39

Birosz, Márton Tamás, Mátyás Andó, and Sudhanraj Jeganmohan. "Finite Element Method modeling of Additive Manufactured Compressor Wheel." Journal of The Institution of Engineers (India): Series D 102, no. 1 (February 13, 2021): 79–85. http://dx.doi.org/10.1007/s40033-021-00251-8.

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AbstractDesigning components is a complex task, which depends on the component function, the raw material, and the production technology. In the case of rotating parts with higher RPM, the creep and orientation are essential material properties. The PLA components made with the material extrusion process are more resistant than VeroWhite (material jetting) and behave similarly to weakly cross-linked elastomers. Also, based on the tensile tests, Young’s modulus shows minimal anisotropy. Multilinear isotropic hardening and modified time hardening models are used to create the finite element model. Based on the measurements, the finite element method simulation was identified. The deformation in the compressor wheel during rotation became definable. It was concluded that the strain of the compressor wheel manufactured with material extrusion technology is not significant.
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40

Liu, Yang, Yan Li, Dejun Wang, and Shaoyi Zhang. "Model Updating of Complex Structures Using the Combination of Component Mode Synthesis and Kriging Predictor." Scientific World Journal 2014 (2014): 1–13. http://dx.doi.org/10.1155/2014/476219.

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Updating the structural model of complex structures is time-consuming due to the large size of the finite element model (FEM). Using conventional methods for these cases is computationally expensive or even impossible. A two-level method, which combined the Kriging predictor and the component mode synthesis (CMS) technique, was proposed to ensure the successful implementing of FEM updating of large-scale structures. In the first level, the CMS was applied to build a reasonable condensed FEM of complex structures. In the second level, the Kriging predictor that was deemed as a surrogate FEM in structural dynamics was generated based on the condensed FEM. Some key issues of the application of the metamodel (surrogate FEM) to FEM updating were also discussed. Finally, the effectiveness of the proposed method was demonstrated by updating the FEM of a real arch bridge with the measured modal parameters.
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41

Mohamed, Mohamed, David Carty, John Storr, Nicola Zuelli, Paul Blackwell, and David Savings. "Feasibility Study of Complex Sheet Hydroforming Process: Experimental and Modelling." Key Engineering Materials 716 (October 2016): 685–91. http://dx.doi.org/10.4028/www.scientific.net/kem.716.685.

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The application of finite element method (FEM) in the area of metal forming and material processing has significantly increased in the recent years. The presented study provides details of the development of a finite element modelling approach to form a part via sheet hydroforming (SHF) process. Both FEM analysis and experimental trials were introduced in this study to produce a complex shape component from Inconel 718 material. The FEM provides a robust feasibility study for forming this part in terms of blank design, load path and process design optimisation. The simulated hydroformed part was validated by performing experimental trials. The analysis demonstrated close correlation between the predicted FE model and the physical trial.
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42

Li, Ruoyu, Jianyao Yao, Linlin Wang, Chen Jiang, Fei Wu, and Ning Hu. "A Novel Gap Element for the Coupling of Incompatible Interface in Component Mode Synthesis Method." International Journal of Computational Methods 17, no. 07 (May 30, 2019): 1950033. http://dx.doi.org/10.1142/s0219876219500336.

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The component mode synthesis (CMS) methods are often utilized for modal analysis to investigate the vibration characteristics of the complex structures which are commonly divided into several substructures. However, non-matching finite element meshes may occur at the interfaces between components and virtual gaps are easily produced along the curved interfaces, which limit the application of CMS and lead to larger numerical errors for vibration analysis. To overcome the problem, a novel gap element method (GEM) is employed into a free-interface CMS method in this paper, where both displacements and forces of the nodes on the incompatible interfaces are introduced by two independent Lagrange multipliers to enforce the compatibility conditions. Two-dimensional numerical examples are given to validate the effectiveness of the proposed method. The results of natural frequencies and modal shapes obtained using the proposed method agree very well with the ones obtained using full finite elements model, no matter the gaps along the interface exist or not. The influence of the number of nodes on the non-matching interfaces on the accuracy of frequencies is also discussed.
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43

Chandra, N., and S. C. Rama. "Application of Finite Element Method to the Design of Superplastic Forming Processes." Journal of Engineering for Industry 114, no. 4 (November 1, 1992): 452–58. http://dx.doi.org/10.1115/1.2900697.

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Finite element method is used as a design tool in the prediction of process parameters for superplastic metal forming processes. The method is used in the design of various plane strain and axisymmetric components. The effect of varying die radii and die friction are studied in the forming characteristics of a simple pan. The cone test used for the mechanical characterization of superplastic materials is simulated. A complex component typically used in the aerospace industry is analyzed and the output pressure-time loading and the resulting thickness distributions are determined.
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44

Lalas, Antonios, Nikolaos Kantartzis, and Theodoros Tsiboukis. "Exploitation of Piezoelectric Micro-Devices as Building Blocks of Controllable Terahertz Complex Materials." Materials Science Forum 856 (May 2016): 135–40. http://dx.doi.org/10.4028/www.scientific.net/msf.856.135.

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A reconfigurable THz complex medium, consisting of fundamental piezoelectric micro-devices, is introduced in this paper. By actuating the piezoelectric modules, a controllable metamaterial, presenting enhanced bandwidth tunability, is accomplished. Two diverse polarization topologies are examined, revealing the anisotropic performance of this material. The advantages of the proposed component are sufficiently clarified through several numerical data, derived by a robust finite element method (FEM).
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45

Jiang, Wei, Kadda Yahiaoui, and Frank R. Hall. "Finite Element Predictions of Temperature Distributions in a Multipass Welded Piping Branch Junction." Journal of Pressure Vessel Technology 127, no. 1 (February 1, 2005): 7–12. http://dx.doi.org/10.1115/1.1845450.

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This contribution deals with the complex temperature profiles that are generated by the welding process in the intersection region of thick walled, cylinder-cylinder junctions. These affect material microstructure, mechanical properties and residual stresses. Knowledge of the thermal history and temperature distributions are thus critical in developing control schemes for acceptable residual stress distributions to improve in-service component behavior. A comprehensive study of three-dimensional temperature distributions in a stainless steel tee branch junction during a multipass welding process is presented. A newly developed partitioning technique has been used to mesh the complex intersection areas of the welded junction. Various phenomena associated with welding, such as temperature dependent material properties, heat loss by convection and latent heat have been taken into consideration. The temperature distribution at various times after deposition of certain passes and the thermal cycles at various locations are reported. The results obtained in this study will be used for on-going and future analysis of residual stress distributions. The meshing technique and modeling method can also be applied to other curved, multipass welds in complex structures.
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46

Liu, Gaolei, Ran Dai, Feng Zhang, Yanpeng Zhao, Chaoyue Zhang, and Fucheng Huang. "Site selection of LNG terminal based on cloud matter element model and principal component analysis." MATEC Web of Conferences 272 (2019): 01027. http://dx.doi.org/10.1051/matecconf/201927201027.

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With the development of liquefied natural gas(LNG) port, as one of the crucial LNG port sitting process, the LNG terminal site’s condition assessment method has always received attention from experts, scholars concern more and more about the method’s practicality and reliability. In the traditional condition assessment method, due to the characteristics of the complex and extensive factors in the comprehensive assessment of the LNG terminal site, the assessment system is not comprehensive enough, or the assessment is too complex, the indexes are not easy to quantify, such problems are emerging. In view of the above reasons, the principal component analysis(PCA) method is used to transform the multi-indicators that affect the comparison of terminal sites into a few comprehensive indicators. A comprehensive evaluation model of the LNG terminal site based on cloud matter element theory and subjective and objective comprehensive weighting method was constructed. By the subjective and objective comprehensive weighting method, the comprehensive weight of each index is determined and the LNG terminal site comprehensive assessment standard cloud element model is constructed with the combination of cloud model and matter-element theory. The cloud matter-element correlation function is established to determine the degree of association between the matter element to be evaluated and the standard cloud matter element model. In order to eliminate random errors and improve the credibility of the results, the algorithm is used for multiple calculations and analysis to achieve the purpose of simultaneously giving the evaluation results and coefficients of credible degree. Finally, the reliability and rationality of the method are verified by an example.
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47

Kaszubska, W., R. Hooft van Huijsduijnen, P. Ghersa, A. M. DeRaemy-Schenk, B. P. Chen, T. Hai, J. F. DeLamarter, and J. Whelan. "Cyclic AMP-independent ATF family members interact with NF-kappa B and function in the activation of the E-selectin promoter in response to cytokines." Molecular and Cellular Biology 13, no. 11 (November 1993): 7180–90. http://dx.doi.org/10.1128/mcb.13.11.7180.

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We previously reported that NF-kappa B and a complex we referred to as NF-ELAM1 play a central role in cytokine-induced expression of the E-selectin gene. In this study we identify cyclic AMP (cAMP)-independent members of the ATF family binding specifically to the NF-ELAM1 promoter element. The NF-ELAM1 element (TGACATCA) differs by a single nucleotide substitution from the cAMP-responsive element consensus sequence. We demonstrate that this sequence operates in a cAMP-independent manner to induce transcription and thus define it as a non-cAMP-responsive element (NCRE). We show that ATFa is a component of the NF-ELAM1 complex and its overexpression activates the E-selectin promoter. In addition, ATFa, ATF2, and ATF3 interact directly with NF-kappa B in vitro, linking two unrelated families of transcription factors in a novel protein-protein interaction. Furthermore, we demonstrate that the ability of overexpressed NF-kappa B to transactivate the E-selectin promoter in vivo is dependent on the NF-ELAM1 complex. Our results suggest that a direct interaction between ATFs and NF-kappa B is, at least in part, the mechanism by which these factors specifically regulate E-selectin promoter activity.
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48

Kaszubska, W., R. Hooft van Huijsduijnen, P. Ghersa, A. M. DeRaemy-Schenk, B. P. Chen, T. Hai, J. F. DeLamarter, and J. Whelan. "Cyclic AMP-independent ATF family members interact with NF-kappa B and function in the activation of the E-selectin promoter in response to cytokines." Molecular and Cellular Biology 13, no. 11 (November 1993): 7180–90. http://dx.doi.org/10.1128/mcb.13.11.7180-7190.1993.

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We previously reported that NF-kappa B and a complex we referred to as NF-ELAM1 play a central role in cytokine-induced expression of the E-selectin gene. In this study we identify cyclic AMP (cAMP)-independent members of the ATF family binding specifically to the NF-ELAM1 promoter element. The NF-ELAM1 element (TGACATCA) differs by a single nucleotide substitution from the cAMP-responsive element consensus sequence. We demonstrate that this sequence operates in a cAMP-independent manner to induce transcription and thus define it as a non-cAMP-responsive element (NCRE). We show that ATFa is a component of the NF-ELAM1 complex and its overexpression activates the E-selectin promoter. In addition, ATFa, ATF2, and ATF3 interact directly with NF-kappa B in vitro, linking two unrelated families of transcription factors in a novel protein-protein interaction. Furthermore, we demonstrate that the ability of overexpressed NF-kappa B to transactivate the E-selectin promoter in vivo is dependent on the NF-ELAM1 complex. Our results suggest that a direct interaction between ATFs and NF-kappa B is, at least in part, the mechanism by which these factors specifically regulate E-selectin promoter activity.
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49

Michelotti, E. F., G. A. Michelotti, A. I. Aronsohn, and D. Levens. "Heterogeneous nuclear ribonucleoprotein K is a transcription factor." Molecular and Cellular Biology 16, no. 5 (May 1996): 2350–60. http://dx.doi.org/10.1128/mcb.16.5.2350.

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The CT element is a positively acting homopyrimidine tract upstream of the c-myc gene to which the well-characterized transcription factor Spl and heterogeneous nuclear ribonucleoprotein (hnRNP) K, a less well-characterized protein associated with hnRNP complexes, have previously been shown to bind. The present work demonstrates that both of these molecules contribute to CT element-activated transcription in vitro. The pyrimidine-rich strand of the CT element both bound to hnRNP K and competitively inhibited transcription in vitro, suggesting a role for hnRNP K in activating transcription through this single-stranded sequence. Direct addition of recombinant hnRNP K to reaction mixtures programmed with templates bearing single-stranded CT elements increased specific RNA synthesis. If hnRNP K is a transcription factor, then interactions with the RNA polymerase II transcription apparatus are predicted. Affinity columns charged with recombinant hnRNP K specifically bind a component(s) necessary for transcription activation. The depleted factors were biochemically complemented by a crude TFIID phosphocellulose fraction, indicating that hnRNP K might interact with the TATA-binding protein (TBP)-TBP-associated factor complex. Coimmunoprecipitation of a complex formed in vivo between hnRNP K and epitope-tagged TBP as well as binding in vitro between recombinant proteins demonstrated a protein-protein interaction between TBP and hnRNP K. Furthermore, when the two proteins were overexpressed in vivo, transcription from a CT element-dependent reporter was synergistically activated. These data indicate that hnRNP K binds to a specific cis element, interacts with the RNA polymerase II transcription machinery, and stimulates transcription and thus has all of the properties of a transcription factor.
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50

Redka, A., V. Bomko, M. Slomchynskiy, O. Cherniavskiy, and S. Babenko. "Digestibility of feed nutrients, nutrient excretion and nutrient retention in broilers under consumption of combined feed with sulfate and zinc-mixed ligand complex." Ukrainian Journal of Ecology 9, no. 3 (October 7, 2019): 156–61. http://dx.doi.org/10.15421/2019_73.

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Abstract:
Digestibility of the feed nutrients and the chemical elements balance in broiler chickens were studied under use of mixed feed with sulfate and Zink-mixed ligand complex in the physiological experiment that was conducted in the vivarium of the Bila Tserkva National Agrarian University. A tendency to increase the level of feed nutrients digestibility in the experimental groups of broiler chickens consuming mixed fodders with Zinc-mixed ligand complex was revealed in the conducted experiment. The nutrients digestibility indices were the highest in the chickens of the experimental group 3 where Zink-mixed ligand complex was added to the mixed feed. 45, 37.5 and 30 g of the element was added per 1 ton of mixed feed, respectively, for the growing period of 5-21, 22-35 and 36-42 days. The protein digestibility in this group was 89.2, fat - 82.5, fiber - 12.9 and Nitrogen free extractive substances (NFES) - 83.8%. The lowest digestibility indices of the nutrients were in the control group chickens, which, depending on the age, consumed feeds added with Zinc sulfate at doses corresponding to the introduction of 60, 50, and 40 g of the element per 1 ton of feed - 60.8, 80.2, 11.27, and 82.2%, respectively. Indicators of Nitrogen, Calcium, Phosphorus, and Zinc deposits in the body were also higher in the chicks of the experimental group 3 consuming the feed with Zink-mixed ligand complex. Feeding the birds with Zinc-mixed ligand complex as a component of mixed feed, in comparison with sulfate, improves the nutrients digestibility and increases the amount of chemical elements deposited in the body, which makes it possible to reduce the dose of the additive.
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