Academic literature on the topic 'Colpa specifica'

La psicopatia č un disturbo di personalitŕ definito dalla presenza di tratti e comportamenti che hanno un impatto negativo sugli individui e sulla societŕ, tra cui egocentrismo patologico, fascino superficiale, senso di sé grandioso, bisogno di stimoli, uso patologico di menzogne e manipolazione, mancanza di rimorso e di senso di colpa, insensibilitŕ, mancanza di empatia, impulsivitŕ, irresponsabilitŕ, tendenza alla criminalitŕ. Sebbene il concetto di psicopatia sia da secoli discusso nella letteratura psichiatrica, la mancanza di una specifica categorizzazione nosografica nei manuali diagnostici, nonché l'erronea sovrapposizione con altri disturbi clinici (ad es., il disturbo antisociale di personalitŕ, o il disturbo narcisistico di personalitŕ nelle sue varianti maggiormente primitive e maligne), hanno contribuito a creare una certa confusione riguardo all'utilizzo appropriato di tale termine. In questo contributo, le caratteristiche della personalitŕ psicopatica vengono confrontate con quelle di altri disturbi di personalitŕ, allo scopo di chiarire quegli aspetti psicopatologici e comportamentali peculiari della psicopatia che consentono di effettuare una diagnosi differenziale rispetto ad altri disturbi psichiatrici. La distinzione tra psicopatia e altri disturbi risulta infatti necessaria, considerato che i soggetti psicopatici sollevano problematiche particolarmente complesse sul piano sociale, dell'assessment clinico e dell'eventuale trattamento, molto diverse da quelle poste da altre categorie diagnostiche con le quali la psicopatia potrebbe essere erroneamente confusa.

Fibrosis is a hallmark of atrial structural remodeling. The main aim of this study was to investigate the role of micro-ribonucleic acids (miRNAs) in the modulation of fibrotic molecular mechanisms in response to hypoxic conditions, which may mediate atrial fibrosis. Under a condition of hypoxia induced by a hypoxia chamber, miRNA arrays were used to identify the specific miRNAs associated with the modulation of fibrotic genes. Luciferase assay, real-time polymerase chain reaction, immunofluorescence and Western blotting were used to investigate the effects of miRNAs on the expressions of the fibrotic markers collagen I and III (COL1A, COL3A) and phosphorylation levels of the stress kinase c-Jun N-terminal kinase (JNK) pathway in a cultured HL-1 atrial cardiomyocytes cell line. COL1A and COL3A were found to be the direct regulatory targets of miR-let-7a, miR-let-7e and miR-133a in hypoxic atrial cardiac cells in vitro. The expressions of COL1A and COL3A were influenced by treatment with miRNA mimic and antagomir while hypoxia-induced collagen expression was inhibited by the delivery of miR-133a, miR-let-7a or miR-let-7e. The JNK pathway was critical in the pathogenesis of atrial fibrosis. The JNK inhibitor SP600125 increased miRNA expressions and repressed the fibrotic markers COL1A and COL3A. In conclusion, MiRNA let-7a, miR-let-7e and miR-133a play important roles in hypoxia-related atrial fibrosis by inhibiting collagen expression and post-transcriptional repression by the JNK pathway. These novel findings may lead to the development of new therapeutic strategies.

By promoting atrial structural remodeling, atrial hypoxia contributes to the development of the atrial fibrillation substrate. Our study aimed to investigate the modulatory effect of hypoxia on profibrotic activity in cultured HL-1 cardiomyocytes and explore the possible signaling transduction mechanisms of profibrotic activity in vitro. Hypoxia (1% O2) significantly and time-dependently increased the expression of hypoxia-inducible factor (HIF)-1α and fibrotic marker proteins collagen I and III (COL1A and COL3A), transforming growth factor (TGF)-β1 and α-smooth muscle actin (SMA). Western blot or immunohistochemistry analysis showed that hypoxia-induced increase in COL1A and COL3A was significantly attenuated by the addition of SP600125 (a specific c-Jun N-terminal kinase [JNK] inhibitor) or expression of dominant-negative JNK before hypoxia treatment. The inhibition of hypoxia-activated phosphorylation of JNK signal components (JNK, MKK4, nuclear c-Jun and ATF-2) by pre-treatment with SP600125 could suppress hypoxia-stimulated HIF-1α upregulation and fibrotic marker proteins expression. Hypoxia significantly increased reactive oxygen species (ROS) production in cultured HL-1 atrial cells. Pre-treatment with N-acetylcysteine significantly abrogated the expression of nuclear HIF-1α, JNK transduction components and fibrotic marker proteins. Taken together, these findings indicated that the hypoxia-induced atrial profibrotic response occurs mainly via the ROS/JNK pathway, its downstream upregulation of HIF-1α and c-Jun/ATF2 phosphorylation and nuclear translocation to up-regulate the expression of fibrosis-related proteins (COL1A, COL3A, TGF-β1 and α-SMA). Our result suggests that suppression of ROS/JNK signaling pathway is a critical mechanism for developing a novel therapeutic strategy against atrial fibrillation.

L'autore esplora e analizza il proprio funzionamento mentale e le proprie modalitŕ relazionali per spiegare al lettore il percorso interiore necessario per superare le situazioni di impasse che mantengono persone e gruppi antagonisti su posizioni antitetiche e inconciliabili. Egli considera la guerra una "situazione patologica" che favorisce il proliferare dei "disturbi della relazione" e suggerisce il ruolo fondamentale che puň essere giocato in modo specifico dagli psicoanalisti e gruppoanalisti e dalle donne nella costruzione di un percorso che, partendo da un conflitto apparentemente insuperabile, arrivi alla capacitŕ di dialogo. Il disturbo relazionale connesso con l'avvio e il mantenimento di situazioni conflittuali č «l'eccesso di identificazione» con la famiglia, la comunitŕ o il gruppo di appartenenza, la nazione. La sola possibilitŕ di superamento del conflitto consiste nel porre fine all'evitamento del senso di colpa, ovvero nell'accettazione delle proprie colpe.

The discovery of experimentally derived induced pluripotent stem cells (iPSCs) has fostered prospects of patient-specific cell replacement therapies, novel toxicology and drug screening assays, and informative cell models for understanding disease pathogenesis. The latter is particularly valuable to the study of human syndromes caused by gene defects where animal models are lacking or inadequately mimic the human condition. One such vascular variant is Ehlers-Danlos Syndrome (EDS), a heritable disease of connective tissues involving collagen types COL5A, COL3A, tenascin-X, and to a lesser extent COL1A, lysyl hydroxlase and ADAM metallopeptidase. Among the six major types, the vascular form of EDS (vEDS) is the most severe. It results principally from mutations in the collagen III, alpha 1 (COL3A1) gene and is thought to cause aberrant collagen fibrillogenesis. These mutations contribute to catastrophic ruptures of large arteries, strokes, pregnancy-related mortalities, and pre-mature death among young adults. The presentation of this disease is heterogeneous, even among patients with the same gene mutation, and it remains unclear why some individuals exhibit severe phenotypes while others do not. In mouse models, COL3A1 deficiency mostly produces a weak disease variant without vascular ruptures, while a spontaneous COL3A1 mutation that leads to vascular defects lacks important disease features found in humans. Currently, there are no adequate therapeutic treatments. To determine how COL3A1 mutations lead to vEDS, we propose to examine and exploit a human cell model of this disease using iPSC lines derived from fibroblasts obtained from two probands. We will correct the Col3A1 gene mutations with TALENs to ensure against phenotypic iPSC variability and determine the principal source of COL3A1 secretion from iPSC-derived vascular progeny. We will examine basic growth characteristics and functional properties of these cells as well as determine the effects of doxycycline on collagen and matrix metalloproteinase synthesis (RNA and protein), stability or activity. By establishing high quality, experimentally confirmed iPSC vascular progeny, this study should lead directly to the development of reliable human tissue models of vEDS suitable for examining the effects of COL3A1 mutations on vasculogenesis and vascular integrity as well as establish a reliable system to test for possible therapeutic (pharmacological or regenerative) interventions.

To identify Ejiao and its adulterants at the DNA level by using DNA molecula marker. Ejiao (Asini Corii Colla) is a commonly used medicinal material. However, its adulteration is a serious concern. Due to the morphological characteristics of Asini Corii Colla and its adulterants, traditional identification techniques often complex and professional, which is not conducive to the circulation management and safety of the medicinal materials. To improve the distinction between Asini Corii Colla and its adulterants accurately, this study identified and its adulterant samples based on the CytB sequence. Sequence characteristics, Basic Local Alignment Search Tool (BLAST) application, genetic distance, construction of phylogenetic tree showed the CytB sequence to accurately identify Asini Corii Colla from its adulterants. Furthermore, in this study, we designed a specific primer, based on the CytB sequence, and established a PCR detection system for rapid, sensitive, and specific identification of Asini Corii Colla. Compared to DNA barcoding technology, this method has shorter detection time, stronger specificity, and higher sensitivity, which lays the foundation for the rapid identification of Asini Corii Colla.

Volgendo l’attenzione alla problematica traduzione del cosiddetto basilicus iactus menzionato nella commedia Curculio di Plauto questo articolo cerca di fornire un nuovo contributo allo studio della cultura ludica latina. Sulla scorta di evidenze interne al testo, in particolare a proposito dell’identità drammatica dei suoi protagonisti e delle isotopie narrative presenti, e grazie a testimonianze esterne alla commedia, nello specifico alcune tradizioni cultuali greche e magno-greche, questa ricerca propone di intendere l’espressione basilicus iactus non come “colpo del re” bensì come “colpo della regina”, con un rinvio implicito alla dea Venus e al colpo degli astragali che dalla divinità prendeva il nome.

Abstract Mechanisms by which leptin stimulates collagen α1(I) [Col1a(I)] gene expression are unclear. The purposes of this study were to identify the trans-acting factors and cis-acting elements in Col1a(I) promoter involved in this effect as well as the pathways that are implicated. In primary cultures of rat hepatic stellate cells (HSCs), we measured the effects of leptin on Col1a(I) gene and protein expression and on the binding of nuclear proteins to the Col1a(I) promoter. We found that leptin increased Col1a(I) gene and protein expression in activated HSCs. Transient transfections showed that leptin exerted its effects through elements located between −220 and −112 bp of the Col1a(I) promoter. Gel retardation assays demonstrated that leptin induced the binding of transcription factors specific protein (Sp)-1 and Sp3 to two elements located between −161 and −110 bp of the Col1a(I) promoter. Leptin-induced Sp1/Sp3 phosphorylation, but this effect was suppressed by inhibiting or silencing Janus kinase-2, phosphatidylinositol-3-kinase, nonphagocytic adenine dinucleotide phosphate (NADPH) oxidase, or ERK1/2, by the use of antioxidants or catalase, or by preventing protein-aldehyde adduct formation. Leptin provoked oxidative stress, aldehyde-protein adduct formation, and increased gene expression of some components of the NADPH oxidase complex. In conclusion, in HSCs, leptin up-regulates Col1a(I) gene expression after activating NADPH oxidase, inducing oxidative stress, aldehyde-protein adduct formation, and ERK1/2 phosphorylation, which in turn activates Sp1/Sp3 and provokes the binding of these two factors to regulatory elements located between −161 and −110 bp of the Col1a(I) promoter. These findings may contribute to a better understanding of mechanisms involved in the leptin-induced liver fibrosis.

Tre diversi movimenti compongono il tempo del lutto: la perdita, la colpa, il perdono. Ciascuno di questi immette nel tempo vissuto dal soggetto uno specifico ritmo e andamento. La morte č figurata come il tradimento per eccellenza ed il lavoro del lutto come un confronto radicale con questo tradimento. Il compiersi del lutto compone la tessitura di una nuova trama relazionale con l'oggetto perduto, quella tela di Penelope, opera del lavoro del lutto e del lavoro della memoria.

Abstract Leaf tissue extracts of Musa acuminata Colla diploid subspecies malaccensis, diploid M. balbisiana Colla, the triploid dessert banana cultivar ‘Valery’, the interspecific hybrid (acuminata × balbisiana) cooking banana cultivars ‘Chato’ and ‘Peli-pita’ and the triploid putative balbisiana-derived cultivars ‘Saba’, ‘Saba Puti’, and ‘Cardaba’ were subjected to isozyme analysis for four different enzymes. Isozyme banding patterns of the interspecific hybrids were generally additive and were a composite of the species-specific forms of each enzyme. Banding patterns for shikimate dehydrogenase, malate dehydrogenase, peroxidase, and phosphoglucomutase indicate that ‘Saba’, ‘Saba Puti’, and ‘Cardaba’ are acuminata × balbisiana hybrids.

La ricerca prende l’abbrivio dalla constatazione della crescente importanza acquisita, nel panorama delle fonti penalistiche, dal fenomeno dell’autonormazione: prodotto del diritto penale post-moderno consistente nell’autoimposizione, da parte dei destinatari stessi della norma, di precetti comportamentali in chiave criminal-preventiva. Oltre al ruolo ambivalente del principio di legalità penale (effetto e causa, al contempo, del fenomeno qui preso in considerazione), l’interesse del penalista per l’approfondimento scientifico del fenomeno è sollecitato dal potenziale che quest’ultimo rivela come alternativa (sostitutiva o integrata) rispetto al diritto penale. Il primo capitolo è dedicato alla ricostruzione delle cause che hanno dato origine al fenomeno, all’uopo ripartite in due macro-categorie: (i) le cause di ordine generale, per l’enucleazione delle quali è stata condotta una ricerca che spazia nelle materie sociologiche, economiche e giusfilosofiche; (ii) le cause di natura giuridica, che sono state investigate considerando sia le manifestazioni comuni all’intero ordinamento giuridico, sia quelle specifiche della penalistica, in cui la crisi del principio della riserva di legge e il declino del diritto penale classico assumono un’importanza cruciale. Nel secondo capitolo, il focus dell’analisi si concentra sulla dimensione strutturale del paradigma autonormativo per come emerso nelle sue principali manifestazioni e nelle concettualizzazioni teoriche maturate soprattutto grazie all’approfondimento riservato al fenomeno della Self-Regulation dagli studiosi di area anglosassone. La paradigmatica dell’autonormazione viene scrutinata tanto nelle sue singole componenti costitutive statiche, quanto nei suoi moti dinamici come strategia regolatoria all’interno dell’ordinamento. La ricerca si sposta nel terzo capitolo dalla struttura alla funzione, con l’obiettivo di ricavare i criteri di politica-criminale strumentali all’impiego dell’autonormazione nel sistema penale. A tal fine, sono state esplorate le possibili relazioni interordinamentali di raccordo tra sistemi autonormativi e ordinamento statale, applicando una metodologia mutuata dall’impostazione di Santi Romano ma ambientata sul terreno del diritto penale e delle sue alternative. Nel quarto capitolo l’indagine si rivolge verso i più eminenti esempi di autonormazione manifestatisi nell’ordinamento italiano: i modelli organizzativi ex D. Lgs. 231 del 2001; i piani per la prevenzione della corruzione nella P.A.; le linee guida medico-chirurgiche per lo svolgimento delle attività sanitaria. Oltre a una disamina ricognitiva della disciplina di questi sub-sistemi normativi, i tre banchi di prova vengono scandagliati in chiave struttural-funzionalistica alla luce dei criteri di analisi illustrati nel secondo capitolo e ricavati nel terzo. Il capitolo 5 chiude il lavoro proiettando i risultati delle ricerche sul piano della teoria del reato, per verificare quale impatto abbia/possa avere l’autonormazione sulla dogmatica. Dopo aver passato in rassegna le possibili ricadute sulle diverse categorie penalistiche, la chiosa finale valorizza il potenziale del diritto riflessivo come candidato ideale per la concretizzazione della clausola di extrema ratio in materia penale. L’uso dell’autonormazione come strumento alternativo rispetto al diritto penale viene ritenuto, infatti, il profilo applicativo più promettente e degno di essere ulteriormente esplorato.
One of the crucial challenges of Criminal Law in the new millennium is to deal with the complexity of contemporary society. The traditional approach based on the State monopoly on criminal matters keeps abreast no longer with the scientific-technological sophistication and the rate of changes in criminal behavior in the era of globalization. In this scenario, we witness the rise of Self-Regulation as an auxiliary tool of crime prevention, whose main goal is to fill the vacuum and to compensate for the rapid obsolescence of state legislation. Compliance Programs, Anti-Bribery Plans, Clinical Guidelines are some of the elements of a diverse constellation of cases in which preventive measures, behavioral rules, surveillance, and sanctions are issued and enforced by a legislator who coincides with the recipient, and which is often a private actor. Nevertheless, the ambivalence of Self-Regulation lies in the fact that – in the face of some positive externalities promised – this paradigm could jeopardize some of the fundamental principles of Criminal Law. The aim of this work is to provide a critical analysis of such phenomenon in order to verify the compatibility of Self-Regulation with the Rule of Law and to assess its efficacy in deterring and detecting misconducts.

Research within translation characterises cultural references as being problematic for translators, meaning they must choose between different translation strategies as to how to transfer these references into the translated text. These strategies are often described in general terms according to how closely oriented they are to the target-culture or source-culture, known as domestication or foreignisation. Translation of literature into English is minimal in world terms and there is seemingly a lack of statistically comparable data concerning the translation of cultural references. This study investigates and compares translation strategies of words denoting cultural references in two translations of the 1998 Swedish Nordic Noir novel Sprängaren by Liza Marklund. The methodology in the study is based on descriptive translation studies, and a categorisation model for types of cultural references is used, as well as an adapted taxonomy model for evaluating translation strategies. Issues addressed are: what types of strategies are used; whether strategies change between translations; how the second translation stands in relation to the retranslation hypothesis and whether any general translation norms are apparent. Results show that a wide variety of translation strategies are used for different types of cultural references, with subtle differences in strategies used in each translation. However, both translations appear to be more domestication-oriented with little overall movement towards to the source culture, as the retranslation hypothesis suggests.

Sensors based on LiDAR (Light Detection and Ranging) technology have the potential to provide accurate 3D models of the trees retrieving information such as canopy volume and height. This information can be used for diagnostics and prescriptions of fertilizers and plant protection products on a site-specific basis. This research aimed to investigate the use of LiDAR sensors in orange crops. Orange is one of the most important tree crop in Brazil. So far, research have developed and tested LiDAR based systems for several tree crops. However, usually individual trees or small field plots have been used. Therefore, several aspects related to data acquisition and processing must still be developed for large-scale application. The first study reported in this document (Chapter 3) aimed to develop and test a mobile terrestrial laser scanner (MTLS) and new data processing methods in order to obtain 3D models of large commercial orange groves and spatial information about canopy geometry. A 2D laser sensor and a RTK-GNSS receiver (Real Time Kinematics - Global Navigation Satellite System) were mounted on a vehicle. The data processing was based on generating a georeferenced point cloud, followed by the filtering, classification and surface reconstruction steps. A 25 ha commercial orange grove was used for field validation. The developed data acquisition and processing system was able to produce a reliable point cloud of the grove, providing high resolution canopy volume and height information. The choice of the type of point cloud classification (by individual trees or by transversal sections of the row) and the surface reconstruction algorithm is discussed in this study. The second study (Chapter 4) aimed to characterize the spatial variability of canopy geometry in commercial orange groves. Understanding such variability allows sensor-based variable rate application of inputs (i.e, applying proportional rates of inputs based on the variability of canopy size) to be considered as a suitable strategy to optimize the use of fertilizers and plant protection products. Five commercial orange groves were scanned with the developed MTLS system. According to the variability of canopy volume found in those groves, the input savings as a result of implementing sensor-based variable rate technologies were estimated in about 40%. The second goal of this study was to understand the relationship between canopy geometry and several other relevant attributes of the groves. The canopy volume and height maps of three groves were analyzed against historical yield maps, elevation, soil electrical conductivity, organic matter and clay content maps. The correlations found between canopy geometry and yield or soil maps varied from poor to strong correlations, depending on the grove. When classifying the groves into three classes according to canopy size, the yield performance and soil features inside each class was found to be significantly different, indicating that canopy geometry is a suitable variable to guide management zones delineation in one grove. Overall results from this research show the potential of MTLS systems and subsequent data analysis in orange crops indicating how canopy geometry information can be used in site-specific management practices.
Sensores baseados em tecnologia LiDAR (Light Detection and Ranging) têm o potencial de fornecer modelos tridimensionais de árvores, provendo informações como o volume e altura de copa. Essas informações podem ser utilizadas em diagnósticos e recomendações localizadas de fertilizantes e defensivos agrícolas. Este estudo teve como objetivo investigar o uso de sensores LiDAR na cultura da laranja, uma das principais culturas de porte arbóreo no Brasil. Diversas pesquisas têm desenvolvido sistemas LiDAR para culturas arbóreas. Porém, normalmente tais sistemas são empregados em plantas individuais ou em pequenas áreas. Dessa forma, diversos aspectos da aquisição e processamento de dados ainda devem ser desenvolvidos para viabilizar a aplicação em larga escala. O primeiro estudo deste documento (Capítulo 3) focou no desenvolvimento de um sistema LiDAR (Mobile Terrestrial Laser Scanner - MTLS) e nova metodologia de processamento de dados para obtenção de informações acerca da geometria das copas em pomares comerciais de laranja. Um sensor a laser e um receptor RTK-GNSS (Real Time Kinematics - Global Navigation Satellite System) foram instalados em um veículo para leituras em campo. O processamento de dados foi baseado na geração de uma nuvem de pontos, seguida dos passos de filtragem, classificação e reconstrução da superfície das copas. Um pomar comercial de laranja de 25 ha foi utilizado para a validação. O sistema de aquisição e processamento de dados foi capaz de produzir uma nuvem de pontos representativa do pomar, fornecendo informação sobre geometria das plantas em alta resolução. A escolha sobre o tipo de classificação da nuvem de pontos (em plantas individuais ou em seções transversais das fileiras) e sobre o algoritmo de reconstrução de superfície, foi discutida nesse estudo. O segundo estudo (Capítulo 4) buscou caracterizar a variabilidade espacial da geometria de copa em pomares comerciais. Entender tal variabilidade permite avaliar se a aplicação em taxas variáveis de insumos baseada em sensores LiDAR (aplicar quantias de insumos proporcionais ao tamanho das copas) é uma estratégia adequada para otimizar o uso de insumos. Cinco pomares comerciais foram avaliados com o sistema MTLS. De acordo com a variabilidade encontrada, a economia de insumos pelo uso da taxa variável foi estimada em aproximadamente 40%. O segundo objetivo desse estudo foi avaliar a relação entre a geometria de copa e diversos outros parâmetros dos pomares. Os mapas de volume e altura de copa foram comparados aos mapas de produtividade, elevação, condutividade elétrica do solo, matéria orgânica e textura do solo. As correlações entre geometria de copa e produtividade ou fatores de solo variaram de fraca até forte, dependendo do pomar. Quando os pomares foram divididos entre três classes com diferentes tamanhos de copas, o desempenho em produtividade e as características do solo foram distintas entre as três zonas, indicando que parâmetros de geometria de copa são variáveis úteis para a delimitação de unidades de gestão diferenciada em um pomar. Os resultados gerais desta pesquisa mostraram o potencial de sistemas MTLS para pomares de laranja, indicando como a geometria de copa pode ser utilizada na gestão localizada de pomares de laranja.

Le succès évolutif de la polyploïdie, notamment de l’allopolyploïdie (où la duplication de génome complet est associée à une hybridation entre génomes différenciés) est en partie lié au fait que cet événement s’accompagne de nombreux changements dans l'organisation du génome et la régulation de l'expression des gènes. On parle du « choc génomique » de l’hybridation interspécifique et de l’allopolyploïdie. Ces sources de diversité génétique, à la fois structurale et fonctionnelle, apparaissent utiles et nécessaires à l'adaptation et l’évolution des espèces. Alors que de nombreuses études portant sur la compréhension des mécanismes moléculaires à l’origine du succès des allopolyploïdes ont concerné les modifications de l’expression des gènes, mes travaux de thèse ont porté sur les agents non codants du génome que sont les éléments transposables et les petits ARN non codants. Le modèle d'étude est le colza (Brassica napus, AACC), espèce allotétraploïde issue de l'hybridation entre les espèces diploïdes navette (B. rapa, AA) et chou (B. oleracea, CC). Nous disposions de colzas néo-synthétisés, étudiés à différentes générations d’autofécondation, permettant de caractériser les changements génomiques accompagnant la formation puis l’évolution du génome néo-allopolyploïde. Une étude a tout d’abord été menée sur un élément transposable (ET) spécifique du génome C, Bot1, en vue d’identifier de nouvelles transpositions survenant chez les colzas néo-synthétisés par rapport aux parents diploïdes, par une approche SSAP. Quelques rares événements de transposition ont été identifiés. Ces résultats, confrontés à ceux obtenus sur deux autres ET, ont permis de mettre en évidence un impact modéré de l’allopolyploïdie sur la transposition de ces différents ET. Par contre, il est apparu que des changements de méthylation auraient accompagné cette allopolyploïdisation, sans doute à l’origine de la réactivation et la transposition de quelques copies de Bot1. Les petits ARN non codants ont été suggérés comme impliqués dans les différents événements génomiques accompagnant la formation d’un génome allopolyploïde. Pour étudier la dynamique d’expression des petits ARN chez des colzas néo-synthétisés pris à deux générations d’autofécondation (S1, S5) en comparaison de leurs parents diploïdes, j’ai exploité des données de séquençage haut débit obtenues pour 11 banques construites à partir des tiges de ces différents génotypes. J’ai ainsi démontré, qu’à une échelle globale, les petits ARN présentaient une réponse immédiate mais transitoire à l’événement d’allopolyploïdie. Les fractions particulièrement affectées par l’allopolyploïdie se sont révélées correspondre (1) à des petits ARN interférents dérivés d’éléments transposables avec une baisse de leur abondance en génération précoce S1, et (2) à des populations de petits ARN de 21 nucléotides exprimées uniquement de manière très précoce, de l’hybride F1 à la génération S1. Nous avons notamment identifié des transcrits de type viral correspondant à ces petits ARN de 21-nt, et présentant les mêmes profils d’expression (de l’hybride F1 à la génération S1), suggérant une réactivation d’éléments viraux endogènes (EVE) en réponse à l’hybridation et l’allopolyploïdie. L’ensemble de mon étude a démontré la mise en place d’une succession des voies de régulation par petits ARN où ET et EVE, réactivés au niveau transcriptionnel, sont immédiatement soumis à une répression post-transcriptionnelle (PTGS), renforcée ensuite par une répression de leur transcription (TGS). L’hypothèse d’une absence de cette régulation par petits ARN lors des phénomènes de nécrose et létalité hybride, amène à envisager ces populations de petits ARN comme les clés de la réussite de la formation d’un génome hybride, où la répression immédiate et efficace des ET et autres endovirus, réactivés suite au choc génomique, se révèle être une nécessité
The evolutionary success of polyploid species is partly due to the dynamic changes in genome organization and gene expression patterns that occur at the onset of the polyploid formation. These changes are promoted by the merging of divergent genomes into a single nucleus (i.e. allopolyploidy) that causes a “genomic shock”; they are thought to provide a rich source of new genetic material upon which selection can act to promote adaptation and evolution. Many studies have thus aimed to uncover molecular mechanisms that are responsible for the evolutionary success of allopolyploid species, most of them focusing on gene expression changes. In the present PhD thesis, my interest has been concentrated on the non-coding components of the genome: transposable elements and small non-coding RNAs. My study involves oilseed rape (Brassica napus, AACC), a relatively young allopolyploid species that originated from hybridizations between B. rapa (AA) and B. oleracea (CC). Specifically, I have used resynthesized B. napus polyploids advanced by self-pollination of single plants for several generations; I have analyzed these plants at different generations for genomic changes accompanying polyploid formation and subsequent evolution. In a first part, sequence-specific amplification polymorphism (SSAP) targeting the C genome-specific transposable element Bot1, was used to evaluate transposition rate of Bot1 in resynthesized B. napus in comparison with the diploid parents. Only a few transposition events were identified. When combined with the results obtained for two other TEs, this work suggests that allopolyploidy has only a moderate impact on TE transposition and restructuring. The changes observed in SSAP profiles led us to hypothesize that some of them resulted from changes in DNA methylation, resulting in rare but highly specific TE activation and transposition. In a second part, I have concentrated on small non-coding RNAs (sRNAs), which are thought to mediate different aspects of the response to the “genomic shock” induced by allopolyploid formation. Comprehensive analyses of sRNA expression in resynthesized B. napus allopolyploids have been carried out by deep sequencing sRNAs from 11 libraries prepared from stems of three allotetraploids (surveyed at the two generations S1 and S5) and the two diploid parents. Characterization of sRNA distributions in these plants indicates that sRNAs show an immediate but transient response to allopolyploidy. The sRNAs derived from transposable elements (down-regulated in the S1) or targeting unknown sequences (no Blast hit against any available public database) were particularly affected. The use of B. napus mRNAseq data revealed that these latest unknown candidates, which are 21-nt long and over-expressed in the earliest generations (F1, S0, S1) were derived from endogenous viral elements (EVE). We confirmed that these EVEs showed the same expression patterns as the 21-nt long sRNAs that specifically target them (over-expression in the F1, S0 and S1). These results suggest that (at least) some EVEs might be reactivated as a response to the merging of divergent genomes (in interspecific hybrids and newly formed allopolyploids). Altogether, our results have demonstrated a succession of sRNA pathways that counteract the reactivation of some specific TEs and/or EVEs at the onset of polyploid formation; reactivated TEs and/or EVEs being immediately repressed at the post-transcriptional level (PTGS), and then fully repressed by transcriptional gene silencing (TGS) in the subsequent generations. Such data lead to hypothesize that sRNAs are essential to overcome interspecific hybrid incompatibilities due to the uncontrolled and deleterious reactivation of TEs / EVEs. Therefore, sRNAs should be considered as the guardians of genome integrity even in newly-formed allopolyploids

Il volume, attingendo a tradizioni disciplinari e storiografiche diverse, offre una serie di sintesi e di affondi tematici, funzionali alla contestualizzazione del peculiare caso dell’episcopato di Bobbio, fondato nel 1014 nell’Appennino piacentino quale sviluppo del celebre monastero di San Colombano. La notevole massa documentaria disponibile grazie al recente riordino degli archivi diocesani, che ha consentito di illustrare anche la biblioteca della cattedrale, ha richiesto una presentazione d’insieme e una specifica attenzione per gli atti costitutivi della nuova istituzione. L’approfondimento del contesto storico entro cui si dipana la successiva vicenda diocesana è stato condotto in rapporto con il monastero e con una selezione delle città vicine. All’analisi delle dinamiche insediative del centro bobbiese e degli sviluppi strutturali della chiesa e del complesso episcopale fanno da contrappunto sia approfondimenti mirati all’organizzazione del territorio, colta nelle sue articolazioni civili, religiose, assistenziali, sia trattazioni di componenti culturali e liturgiche che animano questa Chiesa.

This chapter aims to deconstruct the category of modernity by confronting a prevailing abstracted view on screen advertising with the contingencies of its archival history. Taking as a case study the 1960s ‘cola wars’ and the marketing of cola soft drinks, the chapter shows how this competition between Pepsi and Coke related to stylistic innovations such as montage sequences, and what relevant mid-level finds can be made regarding one specific Pepsi campaign of that era without indulging in overly general arguments about modernism or modernity.

Scientific communities tend to speciate in tribes that evolve their culture so that after some time they do not have the same understanding of the same terms or concepts. Tacit knowledge, that is the knowledge that cannot be expressed or formalized, complicates this even more. Since we cannot fully know what a concept refers to, we would have limitations in developing IT systems to support collaborative and distributed cognition activities and knowledge networks trying to map knowledge between paradigms. To address this, the authors propose to switch to another perspective where knowledge is kept implicit and the referential communication function (iconic signification) is exploited instead. In this perspective, they develop a specific virtual research environment named COLLA-ANT, which, albeit being still a prototype and needing more use cases, has proved successful in addressing the above-mentioned issues.