Dissertations / Theses on the topic 'Colour manipulation'

The purpose of the work is to explore the feasibility of devising a computer system that implements the desirable effects of a photographic filter and provides an environment for colour filter design for image processing. Using conversion from RGB to the CIELUV colour space a new method for the implementation of photographic filter as a digital filter is described. A filter is implemented by converting image pixel rgb values into CIELUV (u', v') and L* values and operates using the visual wavelength values provided by the (u', v') chromaticity diagram. However, the (u', v') diagram cannot provide wavelength values for pixels that correspond to (u', v') points in the `purple line' sector of the diagram. These pixels are allocated wavelengths by means of a new wavelengths scale that makes it possible for the filter to process any pixel in a digital image. Filter transmittance data for visual spectrum wavelengths is obtained from published tables. The transmittance data for purple sector pixels is provided by a colour model of the (u', v') chromaticity diagram. The system is evaluated by means of the Macbeth ColorChecker chart and the use of physical measurements. The extension of the CIELUV diagram with an equivalent wavelength scale provides a new environment for the enhancement and manipulation of digital colour images.

Thesis (MScAgric)--Stellenbosch University, 2000.
ENGLISH ABSTRACT: Red colour development on bi-coloured apples and pears ensures better prices for producers. The use of postharvest irradiation to improve colour has been successful on apples, and the objectives of this thesis were to optimise conditions during irradiation for apples and to evaluate the effects of irradiation on pears. 'Cripp's Pink' apples responded to postharvest irradiation with high-pressure sodium (HPS) lights by developing a red blush, whereas the response to irradiation with UV 8 plus incandescent lights was less effective. '8raeburn' apples held at -0.5°C for 4 or 8 weeks prior to irradiation showed a decrease in hue angle and an increase in anthocyanin concentration after 72 hours of irradiation with HPS lamps. In 'Forelle' pears treated in the same way, neither colour development nor anthocyanin synthesis was affected by irradiation. '8raeburn' and 'Cripp's Pink' apples picked weekly for 5 weeks until the optimum harvest date were irradiated with HPS lights. A significant increase in fruit colour was only measured in mature fruit of both cultivars. 'Cripp's Pink' apples were harvested from two production areas with different microclimates, namely, Ceres and Grabouw, and stored for 0, 2 or 5 days at -0.5 °C before irradiation for 120 hours at either 6°C or 20°C. Fruit from Ceres that were irradiated immediately after harvest developed better colour at 6 °C than at 20°C. The differences between fruit irradiated at the two temperatures were no longer significant after 5 days of cold storage prior to irradiation. Fruit from Grabouw consistently developed better colour when irradiated at 6°C than at 20°C. Colour development slightly after 5 days of cold storage prior to irradiation. In another experiment, fruit from both areas were stored at -0.5°C for 20 days before irradiation at either 6°C or 6/20°C. The fluctuating temperature regime resulted in decreases in hue angle of 70° and 65° for the fruit from Grabouw and Ceres, respectively. The decreases were smaller (±200) when fruit were irradiated at 6°C. The hue angle value of well-coloured 'Cripp's Pink' apples held at 3rC under HPS lights for 144 hours increased from 29.3° to 48.3°, and anthocyanin concentration decreased from 739.9 IJg·g·1to 283.6 IJg·g·1. Control fruit held at the same temperature in the dark did not show any change in hue angle value or anthocyanin concentration. 'Bon Rouge' and 'Red d' Anjou', two full red pear cultivars, irradiated with HPS lights for 72 hours, showed no significant changes in hue angle. 'Forelle' pears, harvested with or without attached leaves, were irradiated with HPS at two temperature regimes, 20°C and 200/6°C. The resulting decreases in hue angle were attributed to yellowing and not red colour formation. In conclusion, the response of apples to postharvest irradiation was affected by maturity and temperature, while pears failed to respond at all.
AFRIKAANSE OPSOMMING: Rooikleurontwikkeling van twee-kleur appels en pere verseker beter pryse vir die produsente. Na-oesbestraling om kleur te verbeter is al suksesvol uitgevoer op appels, en die doelwit van hierdie tesis was om die kondisies vir appels gedurende bestraling te optimaliseer en om die effek van bestraling op pere te evalueer. 'Cripp's Pink' appels het reageer op na-oesbestraling met hoëdruk-natriumligte (HDN) deur 'n rooi blos te ontwikkel, terwyl die reaksie op bestraling met UV-B plus gloeilamplig minder effektief was. 'Braebum' appels opgeberg by -O.5aC vir 4 of 8 weke voor bestraling het 'n afname in die kleurskakeringswaarde getoon, asook 'n toename in antosianienkonsentrasie na 72 uur se bestraling met HDN ligte. 'Forelle' pere wat dieselfde behandeling ontvang het, het geen kleurontwikkeling en geen antosianienontwikkeling getoon na bestraling nie. 'Braebum' en 'Cripp's Pink' appels wat weekliks geoes is vir 5 weke tot die optimum oesdatum is bestraal met HDN ligte. Slegs die volwasse vrugte van beide kultivars het 'n betekenisvolle toename in kleur getoon. 'Cripp's Pink' appels is geoes in twee produksie areas met verskillende mikroklimate, naamlik Ceres en Grabouw. Vrugte is opgeberg vir 0, 2 of 5 dae by -O.5aC voor bestraling vir 120 uur by of 6aC of 20aC. Vrugte van Ceres wat onmiddellik na oes bestraal is het beter kleur ontwikkel by 6aC as by 20aC. Kleurontwikkeling by vrugte bestraal by 6 of 20aC het nie verskil wanneer vrugte vooraf opgeberg was by -~5ac vir 5 dae nie. Vrugte van Grabouw het konstant beter kleur pntwikkel wanneer bestraling by 6°C eerder as 20°C plaasgevind het. In die volgende eksperiment, was beide die vrugte van Ceres en Grabouw vir 20 dae opgeberg by -o.soC voor bestraling by 6°C of 6°/20°C. Die flukturerende temperatuur regime het afnames van 70° en 65° in kleurskakeringswaarde getoon vir die vrugte van Grabouw en Ceres, respektiewelik. Die afname was kleiner (±200)wanneer vrugte by 6°C bestraal is. 'Cripp's Pink' appels wat goed gekleur was en opgeberg is by 3rC terwyl dit blootgestel is aan HDN ligte vir 144 uur, het 'n toename van 29.30 tot 48.3° getoon vir die kleurskakeringswaarde, en antosianienkonsentrasie het afgeneem van 739 I'g.g-1 tot 283.6 I1g.g-1. Die kontrole vrugte opgeberg by dieselfde temperatuur in die donker het geen verandering in beide die kleurskakeringswaarde of die antosianienkonsentrasie getoon nie. 'Bon Rouge' en 'Red d' Anjou', twee volrooi peerkultivars, is bestraal met HDN ligte vir 72 uur en het geen betekenisvolle verandering in kleur getoon nie. 'Forelle' pere, geoes met of sonder 'n aangehegte stingelsegment, is bestraal met HDN ligte by twee verskillende temperatuur regimes, nl. 20°C of 20/6°C. Die afname in kleurskakeringswaarde is aan vergeling toegeskryf eerder as aan rooikleurontwikkeling. Ter opsomming, die reaksie van appels op na-oes bestraling is beïnvloed deur rypheid en temperatuur, terwyl pere geen reaksie getoon het nie.

Thesis (MscAgric (Viticulture and Oenology))--University of Stellenbosch, 2006.
Under certain South African conditions, Redglobe develops a colour that is too dark and thus unacceptable for the Far Eastern markets. These markets require a pink colour instead of a dark red colour. The cultivation of grapes with an acceptable colour involves amongst other, canopy management practices. This generally includes the removal of leaves and/or lateral shoots. Hereby, the leaf area and the microclimatic conditions in the canopy are altered. The aim of this study was to test the usefulness of leaf and lateral shoot removal at different defoliation times after anthesis in order to obtain a pink coloured Redglobe crop. Other quality aspects, namely total soluble solids (TSS), total titratable acidity (TTA), berry mass and total yield, were also evaluated. A canopy management trial was conducted on six year old Redglobe vines with moderate vigour. The treatment design was a 2 x 3 x 4 factorial and involved two leaf removal (L) levels (L0 = 0% leaf removal; L33 = 33% leaf removal) in combination with three lateral shoot removal (LS) levels (LS0 = 0 % lateral shoot removal; LS50 = 50% lateral shoot removal; LS100 = 100% lateral shoot removal). Four defoliation times (DT) were selected: 36 (pea berry size), 69 (véraison), 76 (one week after véraison) and 83 (two weeks after véraison) days after anthesis (DAA). A total of 24 treatment combinations, replicated in four blocks, were applied. Generally, treatment combinations involving 33% leaf removal lowered the main shoot leaf area. Likewise, the lateral shoot leaf area was decreased by increasing levels of lateral shoot removal at any defoliation time. As expected, 33% leaf removal applied in combination with any level of lateral shoot removal, always resulted in a lower total vine leaf area compared to where 0% leaf removal was part of the treatment combination. Compensation reactions occurred and in this regard the main shoot leaf size increased due to 33% leaf removal applied at 1 week after véraison and 2 weeks after véraison. Treatment combinations involving lateral shoot removal increased the ratio of main shoot leaf area to the total leaf area. On the other hand, the main shoot leaf area percentage was lowered by the application of 33% leaf removal at 2 weeks after véraison compared to no leaf removal at the same defoliation time. It can therefore be assumed that the contribution of lateral shoot leaves to grape composition might have increased in cases where the main shoot leaf area was lowered at a later stage (e.g. 2 weeks after véraison). The bunches were visually evaluated and divided into classes from dark (class one) to light (class nine). This visual bunch evaluation showed that the mean bunch colour was in class three (lighter than class two) due to the defoliation time. The lateral shoot removal x leaf removal interaction resulted in a mean bunch colour that was in classes 2 and 3. However, within these classes, there was a tendency that bunch colour decreased for defoliation times later than pea berry size. The lateral shoot removal x leaf removal interactions showed that bunch colour was darker when the treatment combinations involved 0% leaf removal. The percentage of bunches with the desired colour was increased by application of the treatments at véraison, compared to the other defoliation times, and also with 50% lateral shoot removal and 100% lateral shoot removal compared to 0% lateral shoot removal. Biochemical analyses confirmed that increased levels of lateral shoot removal generally lowered the anthocyanin concentration regardless of defoliation time. A similar effect on TSS was observed, i.e. from véraison onwards, the application of 50% lateral shoot removal and 100% lateral shoot removal tended to lower TSS. The effect of these levels of lateral shoot removal at véraison was significant. The role of the lateral shoots in colour development and sugar accumulation is therefore emphasized. Furthermore, the special role that lateral shoots also play in berry development is illustrated in that berry mass tended to decrease when 100% lateral shoot removal in combination with 33% leaf removal and 100% lateral shoot removal in combination with 0% leaf removal were applied at véraison. This, together with the positive relationship obtained between grape colour and the lateral shoot leaf area:fruit mass ratio, accentuates the role of active leaf area during the ripening period. The possible effect of the microclimatic light environment on colour must also be considered. However, although the light intensity increased with increased levels of LS, the colour that was obtained was probably not associated with the differences in light intensity. It was found that it is possible to manipulate the colour of Redglobe grapes with defoliation treatments. However, the treatments that have a decreasing effect on grape colour also affected other quality parameters like TSS and berry size negatively. Although, it is possible to reduce the colour of Redglobe through the application of leaf and lateral shoot removal at different defoliation times, the question arises whether the treatment combinations used in this study are worthwhile to pursue because the mean bunch colour that was obtained was still too dark. However, it was possible to increase the percentage of bunches with the desired colour. Therefore, if such treatments are applied, it must be approached cautiously, keeping in mind that assimilate supply has to be sustained throughout the ripening period.

This work places itself within the field of textile design and atmospheric design. The primary motive is to integrate and broaden the two fields by developing design proposals in textile design. The aim is to explore how to change the atmosphere of a space through the use of fabric manipulations, surface patterns, colours, light and shadow. The design method consisted of material explorations, light and shadow experiments, design of surface patterns, heat transfer print and pleating techniques. Colours were tested in combination with lights. The result is a collection of three textile dividers of space that by the impact of light change the current atmosphere in public spaces, and by doing so, the perception and mood are changed. The value of this work is to show design potentials for new expressional possibilities, a new approach to merge the fields of textile design and atmospheric design.

Trichogramma carverae Oatman and Pinto is mass-released for biological control of the leafroller pest, light brown apple moth, Epiphyas postvittana (Walker) in Australian vineyards. Parasitoid performance can, however, be constrained by a lack of suitable adult food and no information is available on the effect of nectar on the parasitism and longevity of T. carverae. To address this, the effect of alyssum, Lobularia maritima (L.) flowers on E. postvittana parasitism was studied in a vineyard experiment with and without releases of T. carverae. Egg parasitoid activity was assessed with E. postvittana egg �sentinel cards� and no parasitism was recorded in plots without T. carverae releases. Where T. carverae were released, there was no significant enhancement of parasitism by the presence of L. maritima flowers. Three hypotheses were subsequently tested to account for the lack of an effect: (i) T. carverae does not benefit from L. maritima nectar, (ii) T. carverae was feeding on nectar from other flowering plants (weeds) present in the vineyard, (iii) T. carverae was feeding on sugars from ripe grapes. A growth-cabinet experiment using potted L. maritima plants with and without flowers did not support hypothesis one. No parasitism was recorded after day two for T. carverae caged without flowers whilst parasitism occured until day eight in the presence of flowers. A laboratory experiment with common vineyard weeds (Trifolium repens, Hypochoeris radicata, Echium plantagineum) as well as L. maritima did not support hypothesis one but gave partial support to hypothesis two. Survival of T. carverae was enhanced to a small but statistically significant extent in vials with intact flowers of L. maritima, white clover (T. repens) and catsear (H. radicata) but not in vials with flowering shoots of these species from which flowers and flowering buds had been removed. Paterson�s curse (E. plantagineum) flowers had no effect on T. carverae survival. In a laboratory study, punctured grapes significantly enhanced T. carverae survival compared with a treatment without grapes, supporting hypothesis three. Trichogramma carverae performance in the field experiment was probably also constrained by relatively cool and wet weather. Further work on the enhancement of T. carverae efficacy by L. maritima and other carbohydrate sources is warranted. Greenhouse and field experiments were conducted to investigate whether T. carverae benefit from different groundcover plant species. Ten T. carverae adults (<24h after eclosion) were caged with different groundcover species and a control with no plant materials. Epiphyas postvittana egg sentinel cards were used to measure parasitism and longevity was recorded visually. Survival and realised parasitism of T. carverae was significantly higher in L. maritima than in Brassica juncea, Coriandrum sativum, shoots of these species from which flowers had been removed and nil control treatments. A similar experiment with Fagopyrum esculentum (with- and without-flowers) and a control treatment showed that survival was significantly higher in intact F. esculentum than in without-flower and control treatments. There was no significant treatment effect on parasitism in the early stages of that experiment, though parasitism was recorded in the presence of F. esculentum flowers for 12 days, compared with 6 days in other treatments. Higher parasitism was observed in intact Borago officinalis than in the flowerless shoot, water only and no plant material control treatments in a third experiment. There was no significant treatment effect on parasitism. Fitted exponential curves for survival data differed significantly in curvature in the first, second and third experiments but the slope was a non-significant parameter in the second and third experiments. In a second series of laboratory experiments, one male and one female T. carverae were caged with groundcover species to investigate male and female longevity and daily fecundity. Both male and female longevity in F. esculentum and L. maritima treatments were significantly higher than on shoots of these species from which flowers had been removed, and than in the control treatments. Daily fecundity was significantly greater in the intact L. maritima treatment than in all other treatments. Fitted exponential curves for daily fecundity differed significantly in position and slope but not in curvature. There was no significant treatment effect on longevity or parasitism when a male and female were caged with intact B. juncea, B. officinalis or without-flower of these species, nor in the treatment with no plant materials. No parasitism was observed in a survey of naturally occurring egg parasitoids on two sites close to Orange and Canowindra in New South Wales, illustrating the importance of mass releases of T. carverae in biological control of E. postvittana. In an experiment on the Canowindra site, parasitism was significantly higher on day one and day two after T. carverae release when with-flower treatments were compared with without-flower treatments. Parasitism was significantly higher in the F. esculentum treatment than in C. sativum, L. maritima, vegetation without-flowers and control treatments on these dates. On day five, parasitism was higher in C. sativum than in all other treatments. There was no significant increase in parasitism in a second experiment conducted on the Orange site. Coriandrum sativum, F. esculentum and L. maritima appear to be suitable adult food sources for T. carverae and offer some scope for habitat manipulation in vineyards The adults of many parasitoid species require nectar for optimal fitness but very little is known about flower recognition. Flight cage experiments showed that the adults of T. carverae benefited from L. maritima bearing white flowers to a greater extent than was the case for light pink, dark pink or purple flowered cultivars, despite all cultivars producing nectar. Survival and realised parasitism on non-white flowers were no greater than when the parasitoids were caged on L. maritima shoots from which flowers had been removed. The possibility that differences between L. maritima cultivars were due to factors other than flower colour, such as nectar quality, was excluded by dyeing white L. maritima flowers by placing the roots of the plants in 5% food dye (blue or pink) solution. Survival of T. carverae was lower on dyed L. maritima flowers than on undyed white flowers. Mixing the same dyes with honey in a third experiment conducted in the dark showed that the low level of feeding on dyed flowers was unlikely to be the result of olfactory or gustatory cues. Flower colour appears, therefore, to be a critical factor in the choice of plants used to enhance biological control, and is likely to also be a factor in the role parasitoids play in structuring invertebrate communities. Provision of nectar producing plants to increase the effectiveness of biological control is one aspect of habitat manipulation, but care needs to be taken to avoid the use of plant species that may benefit pest species. Greenhouse experiments were conducted to investigate whether the adult E. postvittana and larvae benefit from nectar producing groundcover species. Newly emerged E. postvittana adults were caged with different groundcover species and a honey-based artificial adult diet. The longevity of male and female E. postvittana when caged with shoots of borage (B. officinalis) and buckwheat (F. esculentum) bearing flowers was as long as when fed a honey-based artificial diet. This effect was not evident when caged with shoots of these plants from which flowers had been removed. Longevity was significantly lower than in the artificial diet treatment when caged with coriander (C. sativum) or alyssum (L. maritima) irrespective of whether flowers were present or not. There was no significant treatment effect on the lifetime fecundity of E. postvittana. A second experiment with mustard (B. juncea) (with- and without-flowers), water only and honey-based artificial adult diet showed no significant treatment effects on the longevity of male and female E. postvittana or on the lifetime fecundity of E. postvittana. The anomalous lack of a difference between the water and honey-based diet treatments precludes making conclusions on the value of B. juncea for E. postvittana. Two greenhouse experiments were conducted to evaluate the effects of groundcover species on the larval development of E. postvittana. In the first experiment, larval mortality was significantly higher in C. sativum, and L. maritima than in B. juncea, B. officinalis and white clover (T. repens) a known host of E. postvittana. Coriandrum sativum and L. maritima extended the larval period. In B. juncea and B. officinalis, mortality did not differ from that in T. repens. In F. esculentum, larval mortality was significantly higher than in T. repens. A short larval period was observed on B. juncea, B. officinalis and F. esculentum. Fitted exponential curves for larval mortality differed significantly in curvature between plant treatments. Similarly, successful pupation was significantly lower in C. sativum, F. esculentum and L. maritima than in T. repens. The percentage of successful pupation in B. juncea and B. officinalis did not differ from F. esculentum and T. repens. Fitted exponential curves for pupation differed significantly in curvature. A similar trend was observed in a second experiment with potted plants. The overall results suggest that C. sativum and L. maritima denied benefit to E. postvittana adults and larvae, so could be planted as vineyard groundcover with minimal risk of exacerbating this pest. Overall results suggest that T. carverae require nutrients to reach their full reproductive potential and flowers provide such nutrients. Lobularia maritima and C. sativum may be considered �selective food plants� for T. carverae whereas F. esculentum appears to be a �non-selective food plant�; both T. carverae and E. postvittana benefited from it. Fruits such as grapes can be used as food resources in habitat manipulation and this merits further research. This result also suggests that within species flower colour is an important factor for flower selection in habitat manipulation.

Primad information är lättare tillgängligt i minnet och kan således lättare bli igenkänt. Förutsättningar för priming är bland annat subliminal perception, mål, tillförlitlighet, icke vaksamt och icke vanemässigt. Studiens syfte är att få bredare förståelse gällande subliminal primings påverkan. Frågeställningen var om kunder i en affär kan manipuleras, primas, till att ta en specifik kulör på plastpåse vid kassan samt om det finns någon könsskillnad vid effekten av priming. Deltagarna var 490 kunder, varav 333 män. Två olika skyltar med olika kulörer placerades vid kassan. Det noterades om kunderna valde den primade kulören på plastpåse eller inte. Kontrollgruppen bestod av 117 personer och dessa fick inte se någon skylt. Resultatet visade en signifikant skillnad, deltagarna valde samma kulör på plastpåse som skylten. Inga könsskillnader påträffades. Forskning visar att primingeffekter kan motstridas genom att individen gör sig medveten av potentiell omedveten påverkan.
Primed information is more accessible in memory and can thus easily be recognized. Prerequisites for priming include subliminal perception, goals, reliability, non alert and non habitually. The study aims to gain broader understanding regarding subliminal primings influence. The purpose of this thesis was to see whether the customers in a store could be manipulated, primed, to take a specific colour on plastic bags at checkout and if there are any gender differences in the effect of priming. Participants were 490 customers, of whom 333 men. Two different signs with different colours were placed at the checkout. It was noted if customers chose the primed colour of the plastic bag or not. The results showed a significant difference, the participants chose the same colour on the plastic bag as the sign. No gender differences were found. Research shows that priming effects can be opposed if people make themselves aware of potential unconscious influences.

Dendrobium is the most important cut flower orchid in the world. Understanding the chemical, histological and molecular aspects of flower color is crucial for the development of breeding strategies for novel colors. The objectives of this research were to examine the histology of flower color, cloning and characterization of flavonoid biosynthetic genes, and metabolic engineering of Dendrobium flavonoid pathway to obtain new colors. In Dendrobium, anthocyanins can be confined to a single layer of cells (epidermal or suepidermal) in pale flowers. More intensely colored flowers had anthocyanin in several cell layers. Striped patterns on the perianth were due to the restriction of pigment to cells surrounding the vascular bundles. Color perception is markedly influenced by the presence or absence of carotenoids. Four types of epidermal cells were found in Dendrobium: flat, dome, elongated dome, and papillate. Epidermal cell shape and cell packing in the mesophyll affected the visual texture. Perianth parts with flat cells and a tightly packed mesophyll had a glossy texture, whereas dome cells and loosely packed mesophyll contributed a velvety texture. The labella in the majority of flowers examined had a complex epidermis with more than one epidermal cell shape, predominantly papillate epidermal cells. We were able to isolate a full clone of Dendrobium dihydroflavonol 4-reductase (dfr), and partial clones of chalcone synthase (chs), flavonoid 3'-hydroxylase (f3'h) and flavonoid 3', 5'-hydroxylase (f3'5'h), from Dendrobium Jaquelyn Thomas 'Uniwai Prince' (UH503). Expression data indicated that dfr and chs were expressed to the greatest degree in unopened buds. Amount of f3'h and f3'5'h mRNA was too small to detect. Southern analysis has shown that f3'h and f3'5'h is represented by 2 copies each in UH503. These clones will be extremely useful in future for flower color manipulation. Two different color genes, dfr and f3'5'h from two non-orchid plants, under the constitutive promoter ubiquitin3, were inserted into Dendrobium Icy Pink 'Sakura' with the intention of creating orange-red and blue shades, which are absent in commercial Dendrobium. Presence of the transgene in two sets of transformants was confirmed by PCR. Expression of the transgene from a few plants was indicated by RT-PCR and northern analyses.
Thesis (Ph. D.)--University of Hawaii at Manoa, 2003.
Includes bibliographical references (leaves 148-153).
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The amenability of various color-controlling algorithms to the real-time operator control of color stimuli was investigated. Mathematical models based on eight color spaces were employed: three uniform color spaces (L*u*v*, L*h*C*, and Y 2.2u' VI), a graphics algorithm (HLS), an opponent color model, the NTSC broadcast signals (YIQ), and two sets of color primaries. Eighty subjects, divided equally among the color spaces, were required to match colors under time-limited conditions. The apparatus employed was a color-manipulation device using LEDs, custom-built in the Displays and Controls Laboratory at Virginia Tech. The device allows for 12-bit resolution on each color channel and higher stability of luminance and chrominance over the short and long term than can be achieved with experimental quality CRTs.
Master of Science

A spectrally based normalization methodology (Wpt normalization) for linearly transforming cone excitations or sensor values (sensor excitations) to a representation that preserves the perceptive concepts of lightness, chroma and hue is proposed resulting in a color space with the axes labeled W, p, t. Wpt (pronounced “Waypoint") has been demonstrated to be an effective material color equivalency space that provides the basis for defining Material Adjustment Transforms that predict the changes in sensor excitations of material spectral reflectance colors due to variations in observer or illuminant. This is contrasted with Chromatic Adaptation Transforms that predict color appearance as defined by corresponding color experiments. Material color equivalency as provided by Wpt and Wpt normalization forms the underlying foundation of this doctoral research. A perceptually uniform material color equivalency space (“Waypoint Lab" or WLab) was developed that represents a non-linear transformation of Wpt coordinates, and Euclidean WLab distances were found to not be statistically different from ΔE* ₉₄ and ΔE₀₀ color differences. Sets of Wpt coordinates for variations in reflectance, illumination, or observers were used to form the basis of defining Wpt shift manifolds. WLab distances of corresponding points within or between these manifolds were utilized to define metrics for color inconstancy, metamerism, observer rendering, illuminant rendering, and differences in observing conditions. Spectral estimation and manipulation strategies are presented that preserve various aspects of “Wpt shift potential" as represented by changes in Wpt shift manifolds. Two methods were explored for estimating Wpt normalization matrices based upon direct utilization of sensor excitations, and the use of a Wpt based Material Adjustment Transform to convert Cone Fundamentals to ”XYZ-like" Color Matching Functions was investigated and contrasted with other methods such as direct regression and prediction of a common color matching primaries. Finally, linear relationships between Wpt and spectral reflectances were utilized to develop approaches for spectral estimation and spectral manipulation within a general spectral reflectance manipulation framework – thus providing the ability to define and achieve “spectrally preferred" color rendering objectives. The presented methods of spectral estimation, spectral manipulation, and material adjustment where utilized to: define spectral reflectances for Munsell colors that minimize Wpt shift potential; manipulate spectral reflectances of actual printed characterization data sets to achieve colorimetry of reference printing conditions; and lastly to demonstrate the spectral estimation and manipulation of spectral reflectances using images and spectrally based profiles within an iccMAX color management workflow.

Le contrôle des propriétés et la maîtrise du couplage cohérent d'objets quantiques individuels dans une matrice solide sont un enjeu essentiel pour le développement de l'information quantique. Les centres colorés dans le diamant, équivalent aux molécules artificielles, présentent des caractéristiques qui sont très prometteuses pour la réalisation d'une telle application. Parmi les centres colorés, le centre NV (azote-lacune) est le plus intéressant, dû en particulier à sa grande photostabilité à température ambiante. Le travail de cette thèse est consacré à l'étude des propriétés optiques et électroniques des centres colorés NV à température ambiante.

The endocannabinoid system (ECS) is known to be involved in key aspects of cell maintenance within the human colon, as well as being dysregulated in pathophysiological conditions, including colon inflammation and cancer. However, the contribution of the ECS within each of these conditions has not been fully elucidated. This indicates that the current identification of key targets within the ECS that are involved in gut pathology could be used as potential novel therapeutics. Two experimental approaches were designed and optimised to give an insight into ECS signal regulation within the human colon and to screen ECS therapeutics, tetrahydrocannabinol (THC) and cannabidiol (CBD); a human colon ex vivo explant culture model and an innovative multiplexed quantitative gene expression technology, the GenomeLab GeXP system (Beckman Coulter). Gene targets were identified that are known markers of regulation and function in cells of healthy tissue. An assay, the hCellMarkerPlex was designed that incorporated twenty-three of these gene targets, epithelial (EZR, KRT18, SLC9A2), proliferation (PCNA, CCND1, MS4A12), differentiation (B4GANLT2, CDX1, CDX2), apoptotic (CASP3, NOX1, NTN1), fibroblast (FSP1, COL1A1), structural (ACTG2, CNN1, DES), gene transcription (HDAC1), stem cell (LGR5), endothelial (VWF) and mucin production (MUC2). The hCellMarkerPlex identified gene signatures which distinguished between normal, adenoma and carcinoma tissue, identifying cellular processes showing abnormal activity associated with pathological status. The resulting biomarker profiles were used to establish a human colon explant culture system. The human colon explant culture presents a novel model to study modulation of the ECS and screen ECS therapeutics. Combined with the GenomeLab GeXP System multiple components of the ECS were assessed at the gene regulatory level. A custom designed GeXP assay, the hECSplex, was developed. hECSplex gene expression signatures of EC receptors (CNR1, CNR2, GPR55 and TRPV1), ECS enzymes (NAPE-PLD, GDE1, DAGLA, DAGLB, FAAH, FAAH2 and PTGS2), inflammatory (IL1B, IL10, IL6, LEP, TNF and SOCS3), signalling pathway (ID1, BCL2, CFL1, BIRC5, TP53, MYC and KRAS), lipid production (SREBF1, ACACA), and plasma-membrane (OCLN) markers revealed altered expression of ECS components in carcinogenesis compared to normal tissue. Abstract vi . The hECSplex gene expression signature of colon explants showed that ECS was not altered during culture, emphasising the explant models capability as a pharmaceutical tool to test current and novel therapeutics. Applications of both THC and CBD to normal colon explants at different concentrations do not lead to any significant changes. Indicating the current pharmacological use of phytocannabinoids is causing no adverse effects in surrounding healthy colon tissue. The GenomeLab System presents new opportunities to interrogate multiple components of the endocannabinoid signalling system in small colon explant tissue samples, and in response to ECS therapeutics.

Colorectal neoplasia (CRN) continues to be a leading cause of morbidity and mortality in the developed world and with westernisation, similar trends are now emerging in the developing world. Although secondary prevention through screening programmes has reduced mortality, uptake remains poor due to the invasive nature of colonoscopy, which also exerts increased costs to the health care system. Primary prevention remains the ultimate aim to reduce the morbidity and mortality associated with CRN. In this regard, chemoprevention strategies through regular use of aspirin and other NSAIDS have showed great promise but the associated significant side-effects of these drugs has prevented their routine clinical application for this purpose. Hence there is an urgent need for the identification of safer alternatives for primary prevention of CRN. In parallel to this search, better understanding of the molecular pathogenesis of CRN to identify biomarkers that aid in stratification of at risk individuals would also help. In this regard, the role of chronic inflammation and the influence of host genetics in the pathogenesis of CRN has been the focus of extensive research in recent years. However there is a lack of studies which have investigated these associations in an exclusively screened population, which confers some advantages for this type of investigation. Firstly, most of the screened subjects are relatively healthy, asymptomatic and with no significant co-morbidities, the factors which could otherwise influence the levels of inflammatory markers. Secondly, the screened population is in the 50 to74 year age group which represents the group with a high prevalence of CRN and hence increasing the possibility of finding associations which would be more relevant and generalisable. Thirdly, the selected controls match the cases in all important respects, apart from having CRN, thus increasing the validity of the findings in this population. The Grampian region was one of the first in the UK to participate in the National Colorectal Cancer Screening Programme and this resource gave the ideal opportunity to conduct research involving an exclusively screened population. Utilising this cohort, the current thesis addressed three important aspects of the association between inflammation and CRN. Firstly the investigation of the association of inflammatory genotype, inflammatory phenotype and CRN risk. Secondly the impact of environmental factors, specifically dietary antiinflammatory salicylic acid intakes on CRN risk. And finally assessing if inflammation, and hence in the long term risk of CRN, could be attenuated through a comprehensive anti-inflammatory dietary supplementation in the form of a randomised dietary intervention clinical trial. The study of the association of polymorphisms in key inflammatory genes (IL1B- 31, IL8-251, IL6-174, TNFα-308, IL10-1082, IL10-592, PTGS2-765, and IL1RN VNTR) and CRN risk showed some significant findings. A novel finding was that the homozygous IL1B-31C*C genotype was associated with statistically significant increased risk of CRN, OR 1.63 (95% CI 1.06-2.50) whilst the IL8-251 A*A genotype increased the propensity of having high risk lesions by two-fold (OR 2.04; 95% CI 1.02-4.07). The study of circulating inflammatory marker levels in subjects in whom the CRN was in-situ showed that increased CRP levels were associated with increased risk of CRN, OR 1.55 (95% CI 1.00-2.39). Increased levels of IL8 were associated with increased risk of having a high risk lesion, OR 2.57 (95% CI 1.03-6.44). In a sub group of subjects, it was observed that levels IL8 and CRP decreased following polypectomy (mean IL8 20.3 pg/ml to 14.9 pg/ml, p=0.05 and mean CRP 5.99 mg/l to 3.82 mg/l, p=0.07) raising an important question regarding the sequence of the inflammation-neoplasia cascade, “Is inflammation the cause or the effect of neoplasia?” The study of the association of dietary salicylic acid (SA) and CRN using the newly constructed SA database showed that high levels of total SA (aspirin and dietary SA) intakes were associated with a 75% and moderate levels with a 67% decreased risk of CRN. But dietary SA on its own showed no significant effect on CRN risk probably because of low intake levels in the current cohort. Applying the SA database to populations with higher dietary SA intake would help to further explore its association with CRN risk. The randomised clinical trial examining the effect of a combined antiinflammatory dietary supplement (curcumin, omega-3 PUFA and polyphenols rich fruit smoothie) on markers of inflammation in subjects who had adenomatous colorectal polyps removed showed that the inflammatory marker levels in the control group who just continued their habitual diet remained stable without any statistically significant changes at 6 weeks compared to the baseline. Whereas following 6 weeks of dietary intervention, there was marginally significant increase in IL8 and IL1B levels. One of the possible mechanisms for increase in pro-inflammatory marker levels in the intervention group was the weight gain seen in the intervention group. In the intervention group, the post-intervention mean weight (86.80kgs) was significantly higher than the pre-intervention mean weight (85.38 kgs). In summary, the findings from these investigations suggest that a proinflammatory genotype (IL1B-31C*C and IL8-251 A*A) and elevated circulating inflammatory marker levels (CRP and IL8) are associated with increased risk of CRN. And along with the findings that regular NSAID use and total dietary SA are associated with decreased risk of CRN, our data point to inflammation as an underlying pathogenetic mechanism in CRN. The pilot clinical trial has demonstrated that a clinical trial with combined dietary supplementation is feasible, but challenging. The anti-inflammatory dietary intervention strategy employed to reduce the inflammatory markers did not achieve the desired effect and hence more research is required to establish the ideal delivery strategy of the anti-inflammatory dietary agents. Once this is established, dietary chemoprevention of CRN as a safe alternative should be a realistic achievable goal in the future.

This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Thesis: Ph. D., Massachusetts Institute of Technology, Department of Mechanical Engineering, 2020
Cataloged from student-submitted PDF of thesis.
Includes bibliographical references (pages 66-68).
Optical and photonic fibers represent versatile systems for light manipulation. They are used to guide, reflect, emit, and absorb light, and can be designed to alter the light's spectral composition in any of these light-matter interactions. Additional functionality arises from the combination of these effects in single fibers, and the ability to employ fibers as individual strands, or as woven networks. Two distinct light-manipulating-fiber systems are the focus of this thesis: (1) photonic fibers, which have vivid structural color that changes reversibly in response to mechanical or electrical stimuli, and (2) leaky light guides, which emit light along their length when illuminated from one end. Mechanochromic fibers that convert a mechanical perturbation into an optical response can be used, standalone or integrated into textiles, as easy-to-read strain sensors. Such fibers respond to elongation with a gradual shift in their reflected color through the visible range of light.
In particular, their use in compressive bandages - discussed in detail in this thesis - could greatly improve the efficiency of compression therapy for chronic venous ulcers and other vascular maladies. Electrochromic fibers exploit the electrochemically-tunable absorption of poly(3,4-ethylenedioxythiphene) polystyrene sulfonate, a common conducting polymer, to design devices that can be flipped between a vivid, structurally colored state, and a dull, absorption-colored state. Custom optical multilayer and lumped parameter models are used to analyze the behavior of these fibers. Leaky light guides, by distributing light throughout volumes of algae culture, could yield greater productivity in microalgae cultivation, while lowering energy requirements. The combination of these factors could enable the economically favorable generation of algal biomass for fuels, feedstock, pharmaceuticals, and many other uses.
A passive system for distributing light throughout culture volumes, by selectively scattering light out of light-guiding fibers, is developed and implemented. The process of designing and manufacturing these leaky light guides, and their use in a variety of laboratory-scale bioreactors with live microalgae cultures, are described.
by Joseph D. Sandt.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Mechanical Engineering

Today, there is an increasing demand for smart robots that can make decisions on their own and cooperate with humans in changing environments. The application areas for robotic arms with camera vision are likely to increase in the future of artificial intelligence as algorithms become more adaptable and intelligent than ever. The purpose of this bachelor’s thesis is to develop a robotic arm that recognises arbitrarily placed objects with camera vision and has the ability to pick and place the objects when they appear in unpredictable positions. The robotic arm has three degrees of freedom and the construction is modularised and 3D-printed with respect to maintenance, but also in order to be adaptive to new applications. The camera vision sensor is integrated in an external camera tripod with its field of view over the workspace. The camera vision sensor recognises objects through colour filtering and it uses an edge detection algorithm to return measurements of detected objects. The measurements are then used as input for the inverse kinematics, that calculates the rotation of each stepper motor. Moreover, there are three different angular potentiometers integrated in each axis to regulate the rotation by each stepper motor. The results in this thesis show that the robotic arm is able to pick up to 90% of the detected objects when using barrel distortion correction in the algorithm. The findings in this thesis is that barrel distortion, that comes with the camera lens, significantly impacts the precision of the robotic arm and thus the results. It can also be stated that the method for barrel distortion correction is affected by the geometry of detected objects and differences in illumination over the workspace. Another conclusion is that correct illumination is needed in order for the vision sensor to differentiate objects with different hue and saturation.
Idag ökar efterfrågan på smarta robotar som kan ta egna beslut och samarbeta med människor i föränderliga miljöer. Tillämpningsområdena för robotar med kamerasensorer kommer sannolikt att öka i en framtid av artificiell intelligens med algoritmer som blir mer intelligenta och anpassningsbara än tidigare. Syftet med detta kandidatexamensarbete är att utveckla en robotarm som, med hjälp av en kamerasensor, kan ta upp och sortera godtyckliga objekt när de uppträder på oförutsägbara positioner. Robotarmen har tre frihetsgrader och hela konstruktionen är 3D-printad och modulariserad för att vara underhållsvänlig, men också anpassningsbar för nya tillämpningsområden. Kamerasensorn ¨ar integrerad i ett externt kamerastativ med sitt synfält över robotarmens arbetsyta. Kamerasensorn detekterar objekt med hjälp av en färgfiltreringsalgoritm och returnerar sedan storlek, position och signatur för objekten med hjälp av en kantdetekteringsalgoritm. Objektens storlek används för att kalibrera kameran och kompensera för den radiella förvrängningen hos linsen. Objektens relativa position används sedan till invers kinematik för att räkna ut hur mycket varje stegmotor ska rotera för att erhålla den önskade vinkeln på varje axel som gör att gripdonet kan nå det detekterade objektet. Robotarmen har även tre olika potentiometrar integrerade i varje axel för att reglera rotationen av varje stegmotor. Resultaten i denna rapport visar att robotarmen kan detektera och plocka upp till 90% av objekten när kamerakalibrering används i algoritmen. Slutsatsen från rapporten är att förvrängningen från kameralinsen har störst påverkan på robotarmens precision och därmed resultatet. Det går även att konstatera att metoden som används för att korrigera kameraförvrängningen påverkas av geometrin samt orienteringen av objekten som ska detekteras, men framför allt variationer i belysning och skuggor över arbetsytan. En annan slutsats är att belysningen över arbetsytan är helt avgörande för om kamerasensorn ska kunna särskilja objekt med olika färgmättad och nyans.

A novel flame spray collar called the Coanda Assisted Spray Manipulation collar (CSM) has been tested for use on the Sulzer Metco 5P II combustion flame spray gun. A comparison study of the stock nozzle and the CSM has been performed by evaluating the porosity, surface roughness, microhardness, tensile strength and microscopy of normal and off-normal sprayed NiAl deposits. The use of the CSM collar resulted in the need to position the sprayed coupons closer to the gun, which in turn affected the particle impact energy and particle temperatures of the NiAl powder. For the CSM, porosities had a larger scatterband, surface roughness was comparably the same, microhardness was lower, and tensile strength was higher. The microscopy analysis revealed a greater presence of unmelted particles and steeper intersplat boundaries for the CSM. For both processes, the porosity and surface roughness increased and the microhardness decreased as the spray angle decreased.

In this thesis we examine the feasibility of developing a white light source capable of producing colors between 2500 and 7500 Kelvin on the black-body radiator spectrum by simply adjusting amperage to a blue and ultraviolet (UV) light emitting diode (LED). The purpose of a lighting source of this nature is to better replicate daylight inside a building at a given time of day. This study analyzes the proposed light source using a 385 nm UV LED, a 457 nm blue LED, a 479 nm blue LED, a 562 nm peak cerium doped yttrium aluminum garnet (YAG:Ce) phosphor, and a 647 nm peak selenium doped zinc sulfide (ZnS:Se) phosphor. Our approach to this study initially examined optical performance of yellow-emitting phosphor (YAG:Ce) positioned at specific distances above a blue LED using polydimethylsiloxane (PDMS) as a substrate. An understanding of how phosphor concentration within the PDMS, the thickness of the PDMS, and how substrate distance from the LED die affected light intensity and color values (determined quantitatively by utilizing the 1931 CIE 2° Standard Observer) enabled equations to be developed for various lens designs to efficiently produce white light using a 457 nm peak wavelength LED. The combination of two luminescent sources (457 nm LED and YAG:Ce) provided a linear trend on the 1931 CIE diagram which required a red illumination source to obtain Kelvin values from 2500 to 7500. Red-emitting phosphor (ZnS:Se), selected to compliment the system, was dispersed with YAG:Ce throughout PDMS where they were stimulated with a blue LED thereby enabling all desired Kelvin values with differing concentration lenses. Stimulating ZnS:Se with the addition of a UV LED did not provide the ability to change the color value of the set up to the degree required. Many other factors resulted in the decision to remove the UV LED contribution from the multi-Kelvin light source design. The final design incorporated a combination of ZnS:Se and YAG:Ce stimulated with a blue LED to obtain a 2500 Kelvin value. A separate blue LED provides the means to obtain 7500 Kelvin light and the other color values in between, with a linear approximation, by adjusting the amperages of both LEDs. In addition to investigating the feasibility of obtaining the Kelvin values from 2500 to 7500, this thesis also examined the problem of ZnS:Se’s inability to cure in PDMS and a method to create a lens shape to provide equal color values at all points above a phosphor converted LED source. ZnS:Se was found to be curable in PDMS if first coated with a low viscosity silicon oil prior to dispersion within PDMS. The lens configuration consists of phosphors equally distributed in PDMS and cured in the shape of a Gaussian distribution unique to multiple factors in LED-based white light design.

Le pétrole, le gaz et les minerais sont des ressources indispensables pour le bon fonctionnement de nos économies et sont très convoitées. Elles sont, en outre, particulièrement propices à l’émergence de flux financiers illicites tels que la corruption, l’évitement fiscal ou encore le blanchiment de capitaux. La prise de conscience relative à l’ampleur des pratiques illicites dans le secteur extractif est relativement récente. Depuis une vingtaine d’années, diverses mesures formant un régime juridique ambitieux ont été adoptées pour endiguer ce phénomène. Malgré l’existence de ce régime juridique, les pratiques illicites perdurent au sein de ce secteur. Les acteurs du secteur extractif, entreprises et agents publics, ont su s’adapter et faire preuve de créativité pour contourner, détourner et manipuler ce régime juridique, afin de préserver leurs avantages et de perpétuer les flux financiers illicites. Ils peuvent également recourir à l’ingénierie juridique et financière qui leur fournit un arsenal d’outils juridiques et mobilise des experts maîtrisant à la perfection l’environnement juridique, politique et financier dans lequel les acteurs du secteur extractif évoluent. Ainsi le droit peut-il être propice aux flux financiers illicites et même au service de ceux-ci tout en voulant les combattre
Oil, gas and minerals are indispensable resources for our economies and are sought-after. They are particularly prone to illicit financial flows such as corruption, tax avoidance and money laundering. Awareness of the extent of illicit practices in the extractive sector is relatively new. Over the last two decades, various measures have been adopted, forming an ambitious legal framework to combat illicit financial flows in the extractive sector. Despite the existence of this legal framework, illicit practices are still persistent. Stakeholders within the extractive sector such as companies and public officials have been able to adapt and be creative in order to abuse, circumvent and manipulate this legal framework in order to preserve their advantages and perpetuate illicit financial flows. They can also use legal and financial engineering, which provides them with an arsenal of legal tools and mobilizes experts who master the legal, political and financial environment in which companies and public officials evolve. Hence while wanting to combat illicit financial flows, law can find itself propitious to these illicit practices and even to their service

From an overview of most commonly used kinematic conceptions of robotic manipulators, the conception of Cartesian robot was identified as the most suitable for the given task of colony picking. A control system consisting of two modular parts has been designed for the colony picking robot. ColonyCounter module is a set of image processing libraries for identification of microbial colonies in image data and precise localization of individual colonies. This has been achieved by combination of multiple methods, most importantly connected components labelling and Hough circular transform. The second module – ColonyPicker – utilizes output of ColonyCounter module to plan the picking and placing of colonies. Subsequently it controls the transfer process itself using an innovative task planning and executing system.

Presentation du systeme evita capable d'assister automatiquement l'operateur dans la prevention de toute forme de collision, en restant independant de la commande des engins. Le teleoperateur est averti de toute forme de danger par un signal d'alarme et peut connaitre l'etat du systeme a tout moment grace a la visualisation graphique couleur 3d

This thesis deals with improvement of 3D-manipulator which is currently used for 3D printing. The improvement involves adding a new printer head which will be able to acquire various colors by mixing up to three materials. Furthermore, device is extended with milling as a finishing process responsible for better surface quality of printed object.

博士
國立臺灣大學
光電工程學研究所
102
Using nanostructures to manipulate surface plasmon polariton (SPP) plane waves is an important issue. The interactions of surface nanostructure on SPP wave involve not only the in-plane behavior, but also out-of-plane scattering which is captured as the far-field radiated light. In this dissertation, by using the monochrome laser and multiwavlength laser, the three-dimensional focusing and diverging of SPP waves by a plasmonic structure composed of laser-fabricated gold nanobumps were studied. The forward and backward scattering from individual gold nanobump are observed above and below gold surface, respectively. The gold nanobump structure confers additional three-dimensional propagating wave vectors on SPP wave for departing from surface. A practical application to manipulate the three-dimensional plasmonic scattering is proposed by arranging the gold nanobumps. We manipulate the scattering of SPP waves by various plasmonic structures composed of arranged nanobumps on a gold thin film. Upon controlling the geometry of the plasmonic structures, the height, position, and pattern of scattered light can be modified as desired. It provides a simple and efficient way to project a specific light pattern into free space, and demonstrate the capability of three-dimensional light manipulation. By precisely designing a particular curved structure with appropriate radius of curvature and adjacent interspacing of nanobumps, we can construct a clear single focusing spot at a specific altitude. These results confirm the easy controllability of the focused spot in three-dimensional space by settling the unit curved structures. This research has potential to be applied in the area such as the integrated photonic circuit in the future.

碩士
國立交通大學
光電工程研究所
107
The nano-scale thickness and higher quantum yield compared to other two dimensional materials made transition metal dichalcogenides (TMDCs) a good candidate for realizing ultra-small light emitting devices. However, TMDCs encountered obstacles for practical application since the quantum yields of TMDCs were still relatively low in contrast to traditional semiconductors. Thus, we integrated quantum dots (QDs) with monolayer tungsten diselenide (WSe2), and utilized silver nanodisk (Ag ND) arrays, a kind of plasmonic metasurfaces which could boost absorption and emission at specific resonance frequency, to directly enhance the emission of monolayer WSe2 as well as convert the light from QDs to WSe2. Among the two effects induced by the metasurface, the light conversion or so-called color conversion described the process of one material absorbing the light from other material or source, and then re-emitted the light with absorber’s wavelength, which was a crucial phenomenon used in illumination or display. There were three parts of experiments in the thesis. The first part of experiment will focus on how to realize the Ag ND metasurfaces with desired resonant wavelengths. With the help of FEM simulation as well as bilayer photoresist lift-off process, we designed and fabricated a series of metasurfaces for studying their impact on the color conversion. In the second part, we observed the dependence of plasmonic resonant wavelength. The metasurfaces were made on silica with monolayer WSe2, and their direct enhancement to TMDCs was measured by photoluminescence (PL) system first. Then, we spray coated QDs on them for studying color conversion and found the ideal resonant wavelength located at position between QDs and WSe2 but closer to QDs. At last, we attempted to further increase the conversion efficiency by tuning the metal density of our Ag ND metasurfaces while making all their resonant wavelengths around the ideal position observed in second part. We repeated the measurement in second part and found the density of Ag ND could also affect the enhancement along with conversion efficiency. With higher density came stronger localized surface plasmon effect, which result in the improvement of both effects we got. The successful demonstration of controlling light conversion between QDs and WSe2 through plasmonic metasurfaces suggested that the TMDCs had the potential to be applied to light emitting devices or display technology such as white light emitting diodes (WLEDs) with the assist of localized surface plasmon effect.

碩士
國立成功大學
工業設計學系
105
The little size of the smartphone screen limited the amount of information that can be presented in a single scene, thus the user needs to give more instructions to system for getting corresponding amount of information in the traditional screen, therefore, if the manipulation performance of smartphone can be improved through the design, it can save a lot of unrevealed time of the user. Due to the human performance is primary affected by the ability of the human information process, and the vision is the primary channel for human to receive the exterior information. In the visual performance aspect, the former research indicated that the color is important to influence the human visual performance, and the ability of human information process can be affected through the presentation of information that is changed by colors, thus improving the visual performance. Therefore, the research discussed whether the effect on the visual performance through applying the color can be applied to improve the manipulation performance in the smartphone interface. The research used experimentation as the research method, applying the color combination of font color and background color to change the presentation of number information on screen, and requesting the participant to execute the visual searching and the touch manipulation on the right number. The experiment applied the 7 different hues and the 3 different luminance contrast to construct the color combination as the independent variable, and applied the manipulation time of the participant as the dependent variable, and analyzed the result of experiment through One-way ANOVA statistic analysis. The result of the experiment: in the same luminance contrast combination, the 7 different hues have no significant influence on the manipulation time, indicated that the color combination of experiment have no significant influence on the manipulation performance among the hues; in the same hue, the 3 different luminance contrast combinations have no significant influence on the manipulation time, indicated that the color combination of experiment have no significant influence on the manipulation performance among the luminance contrast combinations. Due to the result of experiment is not consistent with the expectation of the research hypothesis, therefore, discussing the factors that can influence performance such as the variety of color combinations of font color and background color, the size of font, the number of groups, the spacing between information groups, the density of information in the screen, the movement distance of touch manipulation, the balance of the learning effect of the participant, and the test patterns that is applied to balance the movement distance and the learning effect in the experiment, whether the relation of the patterns and the tests is fair enough in design. As a result of the discussing of the factors, the factors that are related to the visual performance and the reaction time applied the suggestion of the former human factor engineering research except the color combination limitation, therefore, the color combination of the research can not induce significant variation among the tests is because of the variety limitation of the color-pairings, or, in the condition that other factors that has been proved do influence performance were adopted in optimization, the color combination of experiment can not affect the manipulation performance in smartphone interface. On the other hand, there is a probability that one of the experiment control variables is the dominant factor that can influence the manipulation performance.

Dissertation submitted in partial compliance with the requirements for the Master's Degree in Technology: Chiropractic, Technikon Natal, 1999.
The aim of this placebo-controlled clinical trial was to determine the role of spinal manipulation in the management of irritable bowel syndrome (IBS), in terms of the patients' subjective response to treatment. It was hypothesized that spinal manipulation would have a greater effect than placebo in reducing the intensity of the symptoms of IBS. Thirty subjects diagnosed with IBS were randomly divided into two groups. Each group consisted of 15 subjects, aged between 18 and 50. Patients were treated twice a week for three weeks and once in the fourth week. Thereafter, each patient returned approximately 1 month later to be assessed for any longer-term benefit to treatment. Patients in the experimental group received spinal manipulation directed at areas of spinal fixation, as determined by motion palpation. Patients in the control group were treated using a detuned ultrasound machine over areas of spinal fixation. Treatment was performed with the same degree of enthusiasm in both groups, where possible.
M

碩士
國立臺灣海洋大學
水產養殖學系
102
The colorchip (PANTONE® formula gude solid uncoated), digital image acquisition and processing (DIAP), and high performance liquid chromatography analysis were used in the present study. In experiment I, 4.09±0.02 g blood parrot were fed with eleven commercial enhanced pigment feeds coming from domestic and foreign countries for two weeks. A control diet without pigment included was made in this study. The questionnaires of the behavior of the ornamental fish consumers were investigated. There were no significant different weigh gain of fish fed control and commercial diets. The astaxanthin concentration of commercial diets ranged from 5.7 to 1164.2 mg/kg. After one week feeding, the astaxanthin concentrations of fish fin of D grouper, the fish skin of C group and the fish muscle of I group showed the highest levels, 26.96 + 1.75, 22.28 + 0.14 and 18.55 + 0.14 mg/kg, respectively. However, after two weeks feeding, the astaxanthin concentrations of fish fin of B group, and the fish skin and fish muscle of E group showed the highest levels, 26.97 + 1.75, 20.74 + 0.37, and 7.6 + 0.17 mg/kg, respectively. Through DIAP observation, the highest level of L*, a*, b*, C* and H* values were found in H group (55), J group (22), D group (30), E group (35) and control group (1.4), respectively. Through color chip observation, the highest level was found in D group (43) and the lowest level was found in K group (16). The questionnaire was distributed to the ornamental fish trade show during October. From this survey, we know the following facts: 1) Taiwanese consumers like to raise small size ornamental fish; 2) they like the colorful ornamental fish; 3) they can afford below 250 NT dollars for 100 g diet; 4) they are used to buy the domestic diets rather than alien diets. In experiment II, 6.41±0.10 g blood parrot were fed diets containing 0, 50 (AX50), 100 (AX100), 150 (AX150), 200 (AX200) and 250 (AX250) mg/kg astaxanthin for three weeks. Fish were fed twice a day (9:00, 17:00) of 2% body weight. After two weeks feeding trial, fish fed AX200 diet had the highest concentration of astaxanthin in fin and skin (18.84±1.73 and 14.08±0.90 mg/kg, respectively). The a* and H* values were optimum in fish fed AX150 diet. In experiment III, 2.87±0.02g gold fish were fed diets containing 0, 25, 50, 75 and 100 mg/kg astaxanthin for four week. Fish were fed 1% body weight diets twice a day (09:00 and 17:00), and photoperiod was maintained 10L:14D in light treatment (AX0-L, AX25-L, AX50-L, AX75-L and AX100-L diets) and 24D in dark treatment (AX0-D, AX25-D, AX50-D, AX75-D and AX100-D diets). Fish fed AX50-L diet showed the highest concentration of astaxanthin in skin and muscle (18.50±2.92 and 27.52±0.57 mg/kg, respectively) after four weeks feeding trial. However, fish fed AX100-L diets showed the highest level of a* and H* values. In dark treatment, the highest concentrations of astaxanthin were found in skin and muscle when fish fed AX50-D and AX25-D diets repectively (19.69±1.17 and 18.78±1.07 mg/kg, repectively), and The a* and H* values were highest in fish fed AX100-D diet.

The goal of this thesis was to assess colour inheritance in Showa tricolour variety of koi carp (black basis, white and red signs). Fish of the same colouration were pair-mated. Colouration of fingerlings was assessed in one day age. Colouration was observed in two forms, like melanin pigmentation and non-melanin pigmentation (black- pigmented and gold -pigmented fingerlings). In the tests, gynogenesis was employed, transmissing only mother`s genome to offspring. Gynogenesis was practiced in RIFCH USB in Vodnany. Gynogenetic offspring was further photographically documented to assess colouration of fingerlings and relationship between both variants of colouration. Fry from propagation was bred in ponds to the size stage of advanced fry (Kr; 90-day old, size 5- 6 cm). In this size, fingerlings were caught from breeding ponds, counted and further documented to check phenotype segregation.

Includes nine pages of amendments.
Bibliography: leaves 140-211.
211, [200] leaves, [12] leaves of plates : ill. (some col.) ; 30 cm.
Aims to determine the reason for the lack of ability of many myeloid leukaemic cell populations to stimulate allogeneic lymphocytes in mixed leucocyte culture (MLC), with a view to manipulating the immunogenicity of these cells for therapeutic purposes.
Thesis (Ph.D.)--University of Adelaide, Dept. of Microbiology and Immunology, 1995

碩士
國立臺灣大學
光電工程學研究所
101
In this thesis, we systematically study the GaN-based LEDs in two aspects: the emission characteristics of semipolar InGaN/GaN quantum well with strain manipulation and the carrier transport of dual color light-emitting-diodes. The first part of the thesis investigates the optical anisotropic behaviour of the (11$ ar{2}$2) and (20$ ar{2}$1) semipolar InGaN/GaN single quantum well LEDs. The influence of different indium compositions of the quantum well, and different degrees of strain relaxation along the projection of $c$-axis are discussed in detail. Our developed one dimensional model is used to solve drift-diffusion, Poisson equations, and 6$ imes$6 $kcdot p$ Schr"{o}dinger equations to investigate the band structures and emission characteristics. The study shows that for the (11$ ar{2}$2)-plane, there exists a switching of light emission polarization directions with the increase of indium composition. While for the (20$ ar{2}$1)-plane, the polarization ratio $ ho_{y''x''}$ can be achieved over 90$\%$ with a high indium composition and a large degree of strain relaxation, which is promising for laser diodes and LCD backlight modules applications. The second part of the thesis will investigate the carrier transport of a novel GaN-based dual color light-emitting diodes (LEDs) with an additional p-GaN layer inserted between the two-color wavelength MQWs. We apply our 2D Poisson, drift-diffusion solver with an extra plug-in function considering the indium fluctuation to make the simulation result more precise. The result shows that by properly selecting the doping density and the thickness of the p-GaN insertion layer, we can effectively optimize the ratio of output light intensities between the dual color wavelength even under a low bias current density.

In this paper, we investigate how controlled changes to image properties and orientation affect eye movements for repeated viewings of images of natural scenes. We make changes to images by manipulating low-level image content (such as luminance or chromaticity) and/or inverting the image. We measure the effects of these manipulations on human scanpaths (the spatial and chronological path of fixations), additionally comparing these effects to those predicted by a widely used saliency model (L. Itti & C. Koch, 2000). Firstly we find that repeated viewing of a natural image does not significantly modify the previously known repeatability (S. A. Brandt & L. W. Stark, 1997; D. Noton & L. Stark, 1971) of scanpaths. Secondly we find that manipulating image features does not necessarily change the repeatability of scanpaths, but the removal of luminance information has a measurable effect. We also find that image inversion appears to affect scene perception and recognition and may alter fixation selection (although we only find an effect on scanpaths with the additional removal of luminance information). Additionally we confirm that visual saliency as defined by L. Itti and C. Koch's (2000) model is a poor predictor of real observer scanpaths and does not predict the small effects of our image manipulations on scanpaths.