Relevant bibliographies by topics / Colar cells

Academic literature on the topic 'Colar cells'

Author: Grafiati
Published: 30 May 2022
Last updated: 31 May 2022

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Journal articles on the topic "Colar cells"

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Wiren, KM, AR Toombs, and XW Zhang. "Androgen inhibition of MAP kinase pathway and Elk-1 activation in proliferating osteoblasts." Journal of Molecular Endocrinology 32, no. 1 (February 1, 2004): 209–26. http://dx.doi.org/10.1677/jme.0.0320209.

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Non-aromatizable androgens have significant beneficial effects on skeletal homeostasis independently of conversion to estradiol, but the effects of androgens on bone cell metabolism and cell proliferation are still poorly understood. Using an osteoblastic model with enhanced androgen responsiveness, MC3T3-E1 cells stably transfected with androgen receptor (AR) under the control of the type I collagen promoter (colAR-MC3T3), the effects of androgens on mitogenic signaling were characterized. Cultures were treated with the non-aromatizable androgen 5alpha-dihydrotestosterone (DHT) and the effects on osteoblast viability were determined as measured by an MTT assay. A complex response was observed in that continuous short-term DHT treatment enhanced osteoblast viability, but with longer-term DHT treatment inhibition was observed. The inhibition by DHT was prevented by the specific AR antagonist hydroxyflutamide, and was also observed in primary cultures of normal rat calvarial osteoblasts. In order to identify potential mediators of this effect, mitogenic pathway-specific cDNA microarrays were interrogated. Reduced hybridization of several genes important in MAP kinase-mediated signaling was observed, with the most dramatic effect on Elk-1 expression. Analysis of phosphorylation cascades demonstrated that DHT treatment inhibited phosphoERK1/2 levels, MAP kinase activation of Elk-1, Elk-1 protein and phosphoElk-1 levels, and downstream AP-1/luciferase reporter activity. Together, these data provide the first evidence that androgen inhibition of the MAP kinase signaling pathway is a potential mediator of osteoblast growth, and are consistent with the hypothesis that the MAP cascade may be a specific downstream target of DHT.
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Kasahara, Takashi, Shigeyuki Matsunami, Tomohiko Edura, Ryoichi Ishimatsu, Juro Oshima, Miho Tsuwaki, Toshihiko Imato, Shuichi Shoji, Chihaya Adachi, and Jun Mizuno. "Multi-color microfluidic electrochemiluminescence cells." Sensors and Actuators A: Physical 214 (August 2014): 225–29. http://dx.doi.org/10.1016/j.sna.2014.04.039.

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Kiberstis, P. A. "Cancer Stem Cells in Color." Science Signaling 5, no. 237 (August 14, 2012): ec212-ec212. http://dx.doi.org/10.1126/scisignal.2003499.

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Kiper, Daniel C., Suzanne B. Fenstemaker, and Karl R. Gegenfurtner. "Chromatic properties of neurons in macaque area V2." Visual Neuroscience 14, no. 6 (November 1997): 1061–72. http://dx.doi.org/10.1017/s0952523800011779.

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AbstractWe recorded from single cells in area V2 of cynomolgus monkeys using standard acute recording techniques. After measuring each cell's spatial and temporal properties, we performed several tests of its chromatic properties using sine-wave gratings modulated around a mean gray background. Most cells behaved like neurons in area V1 and their responses were adequately described by a model that assumes a linear combination of cone signals. Unlike in V1, we found a subpopulation of cells whose activity was increased or inhibited by stimuli within a narrow range of color combinations. No particular color directions were preferentially represented. V2 cells showing color specificity, including cells showing narrow chromatic tuning, were present in any of the stripe compartments, as defined by cytochrome-oxidase (CO) staining. An addition of chromatic contrast facilitated the responses of most neurons to gratings with various luminance contrasts. Neurons in all three CO compartments gave significant responses to isoluminant gratings. Receptive-field properties of cells were generally similar for luminance and chromatically defined stimuli. We found only a small number of cells with a clearly identifiable double-opponent receptive-field organization.
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Rodríguez-Fajardo, Valeria, Vanesa Sanz, Ignacio de Miguel, Johann Berthelot, Srdjan S. Aćimović, Rafael Porcar-Guezenec, and Romain Quidant. "Two-color dark-field (TCDF) microscopy for metal nanoparticle imaging inside cells." Nanoscale 10, no. 8 (2018): 4019–27. http://dx.doi.org/10.1039/c7nr09408f.

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While the applicability of standard DF to detect plasmonic nanoparticles in cells is limited by the scattering from the cell's compartments, TCDF overcomes this limitation by using the scattering information of two colors.
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ROUHI, MAUREEN. "Coral Color Shift Applied To Cells." Chemical & Engineering News 80, no. 39 (September 30, 2002): 8. http://dx.doi.org/10.1021/cen-v080n039.p008a.

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Kolb, H., and P. K. Ahnelt. "Color connectivity of human horizontal cells." Experimental Eye Research 55 (September 1992): 239. http://dx.doi.org/10.1016/0014-4835(92)91044-x.

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Sinkorova, Z., J. Sinkora, L. Zarybnicka, Z. Vilasova, and J. Pejchal. "Radiosensitivity of peripheral blood B cells in pigs." Veterinární Medicína 54, No. 5 (June 1, 2009): 223–35. http://dx.doi.org/10.17221/59/2009-vetmed.

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: Swine are here introduced to biodosimetry in an attempt to develop a large animal model allowing for comparison of <I>in vitro</I> experiments with the <I>in vivo</I> processes occurring after exposure to gamma radiation. This work investigates the radiosensitivity of the B cell compartment in peripheral blood. Four-week-old piglets were irradiated using the whole body protocol or full blood samples were irradiated <I>in vitro</I> in the dose range of 0–10 Gy. Relative radioresistance of B cell subpopulations and subsets was determined by measuring their relative numbers in leukocyte preparations at selected time intervals after irradiation using two color immunophenotyping and flow cytometry. Porcine B cells represent the most radiosensitive lymphocyte population in peripheral blood. Among B cell subpopulations and subsets investigated, the CD21+SWC7+ and CD21+CD1+ cells are highly radiosensitive and possess biodosimetric potential, at least in the range of low doses. Differences between cultures irradiated <I>in vitro</I> and lymphocyte dynamics in peripheral blood of irradiated animals clearly document the limits of <I>in vitro</I> data extrapolation in biodosimetry. We have shown that pigs can successfully be used in radiobiology and experimental biodosimetry due mainly to their availability, size and a relatively broad spectrum of available immunoreagents for lymphocyte classification.
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Sakai, Hiroko M., Hildred Machuca, and Ken-Ichi Naka. "Processing of Color- and Noncolor-Coded Signals in the Gourami Retina. II. Amacrine Cells." Journal of Neurophysiology 78, no. 4 (October 1, 1997): 2018–33. http://dx.doi.org/10.1152/jn.1997.78.4.2018.

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Sakai, Hiroko M., Hildred Machuca, and Ken-Ichi Naka. Processing of color- and noncolor-coded signals in the gourami retina. II. Amacrine cells. J. Neurophysiol. 78: 2018–2033, 1997. The same set of stimuli and analytic methods that was used to study the dynamics of horizontal cells ( Sakai et al. 1997a ) was applied to a study of the response dynamics and signal processing in amacrine cells in the retina of the kissing gourami, Helostoma rudolfi. The retina contains two major classes of amacrine cells that could be identified from their morphology: C and N amacrine cells. C amacrine cells had a two-layered dendritic field, whereas N cells had a monolayered dendritic field. Both types of amacrine cell were tracer-coupled but coupling was more extensive in the N amacrine cells. Responses from C amacrine cells lacked a DC component and had a small linear component that was <10% in terms of mean square error (MSE); the second-order component often accounted for >50% of the modulation response. The C amacrine cells did not show any characteristic color coding under any stimulus condition. Most responses of N cells to a pulsatile stimulus consisted of a series of depolarizing transient potentials and steady illumination did not generate any DC potential in these cells. The response to a white-noise modulated input was composed of well-defined first- and second-order components and, possibly, higher-order components. The response evoked by a red or green white-noise–modulated stimulus given alone was not color coded. Modulated red illumination in the presence of a green illumination elicited a color-coded response from >70% of N amacrine cells. Color information was carried not only by the polarity but also by the dynamics of the first-order component. No convincing evidence was obtained to indicate that the second-order component might be involved in color processing. Some N amacrine cells produced a well-defined (second-order) interaction kernel to show that the temporal sequence of red and green stimuli was a parameter to be considered. In a complex cell such as an amacrine cell, responses evoked by a pulsatile stimulus given in darkness and by modulation of a mean luminance could be very different in terms of their characteristics. It was not always possible to predict the response evoked by one stimulus from observing the cell's response to another stimulus. This is because, in N cells, a flash-evoked (nonsteady state) response is composed largely of nonlinear components whereas a modulation (steady state) response is composed of linear as well as nonlinear components.
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Sato, H., N. Katsuyama, H. Tamura, Y. Hata, and T. Tsumoto. "Broad-tuned chromatic inputs to color-selective neurons in the monkey visual cortex." Journal of Neurophysiology 72, no. 1 (July 1, 1994): 163–68. http://dx.doi.org/10.1152/jn.1994.72.1.163.

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1. Input mechanisms of 21 color-selective cells in cytochrome oxidase-rich blobs in layer II/III of the anesthetized and paralyzed monkey primary visual cortex were studied by an iontophoretic administration of the GABAergic receptor antagonist bicuculline methiodide (BMI). 2. Color-selective blob cells become responsive to originally nonresponsive colors of stimuli or brightness contrast stimuli during removal of intracortical inhibition. 3. The magnitudes of the cells' responses to color stimuli during BMI administration were larger than the expected value of response calculated from the previously reported color tuning of color-selective geniculate cells and emission spectra of color stimulus. 4. These results suggest that color-selective blob cells receive a convergence of different types of chromatic inputs and that intracortical inhibition confers selectivity for a given color on them.
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Dissertations / Theses on the topic "Colar cells"

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Мадан, Роман Григорович. "Фотоперетворювачі на основі наноструктурованого кремнію." Bachelor's thesis, КПІ ім. Ігоря Сікорського, 2019. https://ela.kpi.ua/handle/123456789/28855.

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Робота складається з 55 сторінок, 4 розділів та містить 35 ілюстрацій, 24 таблиці та 19 джерел в переліку посилань. Актуальність теми – інтерес до створення гібридних органічних та неорганічних фотоперетворювачів, що мають більш низьку вартість, ніж традиційні. Метою роботи є дослідження вольт-амперних характеристик фотоперетворювачів. Порівняння характеристик пористого кремнію, отриманого за різного часу травлення. Об’єкт дослідження – фотоперетворювачі на основі наноструктурованого кремнію. Предмет дослідження – методи одержання та морфологія наноструктурованого шару оксиду індію й олова, а також плівки меланіну.
The work consists of 55 pages, 4 sections and contains 35 illustrations, 24 tables and 19 sources in the list of references. The actuality of the topic is the interest in the creation of hybrid organic and inorganic photoconductors that have a lower cost than traditional ones. The purpose of the work is to study the volt-ampere characteristics of nanostructured silicon solar cells. Comparison of the characteristics of porous silicon obtained at different times of etching. The object of research is nanostructured silicon solar cells. Subject of research - methods of obtaining and morphology of nanostructured layer of indium and tin oxide, as well as melanine films.
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Szmajda, Brett A. "Subcortical pathways for colour vision /." Connect to thesis, 2006. http://eprints.unimelb.edu.au/archive/00003165.

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Thesis (Ph.D.)--University of Melbourne, The National Vision Research Institute of Australia and Dept. of Optometry & Vision Sciences, 2007.
Typescript. Includes bibliographical references (leaves 95-111).
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Pedron, Ilario. "Digital image processing for cancer cell finding using color images." Thesis, McGill University, 1988. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=61720.

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Andrew, Harold Gibbons. "Design and Application of Optical Polymers." Kyoto University, 2019. http://hdl.handle.net/2433/242585.

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Pino, Javier. "Transgneic Endothelin 3 Regulates Murine Pigment Production and Coat Color." FIU Digital Commons, 2017. https://digitalcommons.fiu.edu/etd/3533.

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Pigmentation plays a protective role against damage caused by ultraviolet (UV) irradiation. Humans with fair skin and light hair have a higher susceptibility to UV-induced DNA damage that can lead to the development of skin cancers. The melanocytes found in the skin and hair follicles depend on different signaling molecules for their proper development and pigment production. α-Melanocyte Stimulating Hormone (α-msh) binds to the Melanocortin 1 receptor (Mc1r) to regulate pigment production and the switch between eumelanin and pheomelanin. Lethal yellow mice (Ay) overexpress the agouti signaling protein, which inhibits the binding of α-msh, resulting in a yellow coat color phenotype. Endothelin 3 (Edn3) encodes for a ligand involved in melanocyte development by regulating the differentiation, proliferation and migration of melanocyte precursors. A tetracycline inducible transgenic mouse in which Edn3 was placed under the keratin 5 promoter (K5-tTA;TRE-Edn3-lacZ) displays a hyperpigmentation phenotype due to the accumulation of melanocytes in the skin and an increase in hair pigment. Comparative analysis of dorsal hairs from Ay and Ay; K5-tTA;TRE-Edn3-lacZ mice using high performance liquid chromatography showed that transgenic Edn3 expression significantly increased both eumelanin and pheomelanin. No significant difference in the number of follicular melanocytes between Edn3 transgenic and non-transgenic mice was evidenced by immunofluorescence using an antibody against Tyrosinase related protein 1. Gene expression analysis of hair follicles showed that Edn3 upregulates the expression of melanogenic genes. Deactivation of transgenic Edn3 is possible with doxycycline (dox) treatment. To test if transgenic Edn3 expression is required to rescue and maintain a dark pigmentation phenotype in Ay mice, dox was administered during embryonic and postnatal development to manipulate transgenic Edn3 expression. Results showed that transgenic Edn3 expression is required to maintain a dark pigmentation phenotype after birth but is independent of a developmental requirement. Transgenic Edn3 expression in Mc1re/e mice also resulted in a darkened coat color. Our results indicate that the paracrine expression of Edn3 from keratinocytes is capable of generating and maintaining a dark coat color by the regulation of melanogenic genes independent of Mc1r signaling. The results of this study may open new approaches to the treatment of hypopigmentation disorders.
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Vedin, Joel. "Utilizing an efficient color-conversion layer for realization of a white light-emitting electrochemical cell." Thesis, Umeå universitet, Institutionen för fysik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-122097.

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Organic semiconducting materials have received a lot of attention in recent years and can now be found in many applications. One of the applications, the light emitting electrochemical cell (LEC) has emerged due to its flat and lightweight device structure, low operating voltage, and possibility to be fully solution processed. Today LECs can emit light of various colors, but to be applicable in the lighting industry, white light need to be produced in an efficient way. White light on the other hand, is one of the toughest "colors" to achieve in an efficient way, and is of particular interest in general lighting applications, where high color-rendering index devices are necessary. In this thesis I show that blue light can be partially converted, into white light, by utilizing the photoluminescence of color conversion layers (CCLs). Furthermore, I show that a high color-quality white light can be attained by adopting a blue-emitting LEC with a CCL. Particularly, three different color-conversion materials were embedded onto a blue bottom-emitting LEC, to study the resulting spectrum. One of the materials, MEH-PPV, have good absorption compatibility with the electroluminescence of the blue emitters, but the materials photoluminescence do not cover the red to deep-red range of the spectrum. These parts of the spectrum are necessary to obtain high color rendering indices (≥80). A single layer of MEH-PPV adapted onto a blue-emitting LEC, led to a cold white LEC with CIE-coordinates x = 0.29, and y = 0.36, color-rendering index = 71, and correlated color temperature = 7200 K. These properties makes it potentially useful in outdoor-lighting applications. The photoluminescence of another studied color-converting material, polymer red, covers the red to deep-red range of the spectrum but the material lacks absorption in the green parts of the blue emitters electroluminescence spectrum. Thus it is necessary to combine it with MEH-PPV to be able to absorb all wavelengths from the blue-emitter and get a broad light-spectrum out of the device. In order to preserve a part of the blue light, a new device configuration was designed. It features a top-emitting blue LEC with a dual-layer CCL which reach an impressive color rendering index = 89 at a correlated color temperature = 6400 K (CIE-coordinates x = 0.31, y = 0.33). The color-rendering index is the highest reported for a white LEC. The absence of UV-, and IR-radiation, together with the high color rendering properties make the white LEC a possible candidate for even the most demanding lighting-applications, such as art galleries, and shop display windows, together with indoor lighting. In this thesis, I show that the CCLs function well. However, for the LECs to be worthy competitors, the efficiency and lifetime of the blue emitter need improvements.
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Pinidiyaarachchi, Amalka. "Digital Image Analysis of Cells : Applications in 2D, 3D and Time." Doctoral thesis, Uppsala universitet, Centrum för bildanalys, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9541.

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Light microscopes are essential research tools in biology and medicine. Cell and tissue staining methods have improved immensely over the years and microscopes are now equipped with digital image acquisition capabilities. The image data produced require development of specialized analysis methods. This thesis presents digital image analysis methods for cell image data in 2D, 3D and time sequences. Stem cells have the capability to differentiate into specific cell types. The mechanism behind differentiation can be studied by tracking cells over time. This thesis presents a combined segmentation and tracking algorithm for time sequence images of neural stem cells.The method handles splitting and merging of cells and the results are similar to those achieved by manual tracking. Methods for detecting and localizing signals from fluorescence stained biomolecules are essential when studying how they function and interact. A study of Smad proteins, that serve as transcription factors by forming complexes and enter the cell nucleus, is included in the thesis. Confocal microscopy images of cell nuclei are delineated using gradient information, and Smad complexes are localized using a novel method for 3D signal detection. Thus, the localization of Smad complexes in relation to the nuclear membrane can be analyzed. A detailed comparison between the proposed and previous methods for detection of point-source signals is presented, showing that the proposed method has better resolving power and is more robust to noise. In this thesis, it is also shown how cell confluence can be measured by classification of wavelet based texture features. Monitoring cell confluence is valuable for optimization of cell culture parameters and cell harvest. The results obtained agree with visual observations and provide an efficient approach to monitor cell confluence and detect necrosis. Quantitative measurements on cells are important in both cytology and histology. The color provided by Pap (Papanicolaou) staining increases the available image information. The thesis explores different color spaces of Pap smear images from thyroid nodules, with the aim of finding the representation that maximizes detection of malignancies using color information in addition to quantitative morphological parameters. The presented methods provide useful tools for cell image analysis, but they can of course also be used for other image analysis applications.
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Baum, Michael [Verfasser], and Karsten [Akademischer Betreuer] Rippe. "Protein Mobility and Interaction Measurements in Living Cells by Dual-Color Multi-Focus Fluorescence Cross-Correlation Spectroscopy / Michael Baum ; Betreuer: Karsten Rippe." Heidelberg : Universitätsbibliothek Heidelberg, 2014. http://d-nb.info/1179925017/34.

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Kumar, Sarker Shuronjit. "Textile wastewater treatment and electricity generation by Microbial Fuel Cell with freezing technology as pre-treatment (A No-water discharge approach)." Thesis, KTH, Mark- och vattenteknik (flyttat 20130630), 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-171813.

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Textile wastewater contains very high concentration of color, COD, suspended solids and other pollutants. Methods such as reverse osmosis, nano-filtration and ultrafiltration are known to be effective to remove some pollutants but these methods are very expensive. A new treatment approach which is the combination of freezing technology and Microbial Fuel Cell technology has been studied in this thesis work and seems to have great potential to remove color and COD from textile wastewater. Freezing splits a diluted stream into two different streams; one stream in which water is transferred into ice with a low pollutant concentration leaving a concentrated stream with pollutants. Microbial fuel cell uses the concentrated stream to convert biochemical energy into electrical energy. Three different types of substrates, KMnO4 solution, municipal wastewater and orange juice, were studied. Freezing technology can produce high quality water by neutralizing pH-value; close to 7.0, removal of COD is more than 95% and separating color by almost 100%. Similarly MFC can remove color, and COD by 88.8% and 73.6% respectively. The maximum generation of electrical power by MFC was estimated to 1.03 mW/m2 of electrode area. The findings suggest that this new approach of textile wastewater treatment can be a costeffective way to remove pollutants from textile wastewater while generating some electricity.
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Arriaga, Odra O. "Nature, Essence and Spirit| An Artistic Process of Space, Lines and Color from the Level of Cells to the Large Oceanic Water World." Thesis, The University of Texas Rio Grande Valley, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10263731.

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The methods, experimentations, philosophies and influential artists mentioned in this thesis all form part of my artistic exploration and art from the level of cells to the large oceanic water world. As an artist of Fine Arts at the University of Texas, Rio Grande Valley, my main emphasis is to associate these concepts with my personal experiences, cultural traditions and artistic perspectives with my likes for lines, colors and spaces in what I called, Nature, Essence and Spirit. Furthermore, my artistic approach was mostly influenced by the views of artists such as, Vasily Kandinsky, Frank Stella, Piet Mondrian, Paul Klee, Dale Chihuly and Tauba Auerbach.

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Books on the topic "Colar cells"

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Kuehnel, Wolfgang. Color atlas of cytology, histology, and microscopic anatomy . 4th ed. Stuttgart: Thieme, 2003.

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Color atlas of cancer cytology. 3rd ed. Tokyo: Igaku-Shoin, 2000.

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Color atlas of cytology, histology, and microscopic anatomy. 4th ed. Stuttgart: Thieme, 2003.

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Herzberg, Arlene J. Color atlas of normal cytology. New York: Churchill Livingstone, 1999.

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Theml, Harald. Color atlas of hematology: Practical microscopic and clinical diagnosis. [electronic resource]. 2nd ed. Stuttgart: Thieme, 2004.

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Frithjof, Hammersen, and Sobotta Johannes 1869-1945, eds. Histology, color atlas of microscopic anatomy. 3rd ed. Baltimore: Urban & Schwarzenberg, 1985.

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Hawkey, C. M. Color atlas of comparative veterinary hematology: Normal and abnormal blood cells in mammals, birds and reptiles. Ames, [Iowa]: Iowa State University Press, 1989.

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Fournel-Fleury, Corinne. Atlas en couleur de cytologie du cancer chez le chien et le chat =: Color atlas of cancer cytology of the dog and cat. Paris: Conférence nationale des vétérinaires spécialisés en petits animaux, 1994.

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1926-, Naylor Bernard, ed. Practical guide to surgical pathology with cytologic correlation: A text and color atlas. New York: Springer-Verlag, 1992.

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Ransom, Bruce R. Neuroglia. Edited by Helmut Kettenmann. Oxford University Press, 2013. http://dx.doi.org/10.1093/med/9780199794591.001.0001.

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This resource is the long-awaited new revision of the most highly regarded reference volume on glial cells, and has been completely revised, greatly enlarged, and enhanced with full color figures throughout. Neglected in research for years, it is now evident that the brain only functions in a concerted action of all the cells, namely glia and neurons. Seventy one chapters comprehensively discuss virtually every aspect of normal glial cell anatomy, physiology, biochemistry and function, and consider the central roles of these cells in neurological diseases including stroke, Alzheimer disease, multiple sclerosis, Parkinson's disease, neuropathy, and psychiatric conditions. With more than 20 new chapters it addresses the massive growth of knowledge about the basic biology of glia and the sophisticated manner in which they partner with neurons in the course of normal brain function.
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Book chapters on the topic "Colar cells"

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Valberg, Arne. "Ganglion cells." In Encyclopedia of Color Science and Technology, 678–79. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4419-8071-7_276.

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Valberg, Arne. "Ganglion cells." In Encyclopedia of Color Science and Technology, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-642-27851-8_276-2.

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Valberg, Arne, and Jan Kremers. "Ganglion Cells." In Encyclopedia of Color Science and Technology, 1–3. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-642-27851-8_276-3.

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Valberg, Arne. "Ganglion Cells, On-Center Ganglion Cells, Off-Center Ganglion Cells, Midget Ganglion, Diffuse Ganglion Cells." In Encyclopedia of Color Science and Technology, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-27851-8_276-1.

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Davies, Derek, and Clare Hughes. "Dead Cell Discrimination." In In Living Color, 372–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57049-0_30.

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Diamond, Rochelle A. "Electronic Cell Sorting." In In Living Color, 563. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57049-0_46.

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Ikeda, Yuzuru. "Color Change in Cephalopods." In Pigments, Pigment Cells and Pigment Patterns, 425–49. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-1490-3_14.

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Rodieck, R. W. "Which Cells Code for Color?" In From Pigments to Perception, 83–93. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3718-2_10.

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Morrison, Sean J. "Purification of Mouse Fetal liver Hematopoietic Stem Cells." In In Living Color, 585–91. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57049-0_50.

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Leif, Robert C. "Methods for Preparing Sorted Cells as Monolayer Specimens." In In Living Color, 592–619. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57049-0_51.

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Conference papers on the topic "Colar cells"

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Li, Wei, and Daniel A. McAdams. "Novel Pixelated Multicellular Representation for Origami Structures That Innovates Computational Design and Control." In ASME 2013 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/detc2013-13231.

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Origami is a traditional Asian fine art of creating three-dimensional structures by folding paper. Recently, engineers have started to exploit the functional advantages of foldable structures. Formal representations of origami structures are limited. Most of the origami research describes an origami structure by providing the geometric properties of creases and vertices. This paper proposes a novel representation of an origami structure by describing the faces. An origami sheet is first pixelated with evenly distributed cellular agents, referred to as cells. Pixelization makes the origami structurally analogous to an LED matrix screen, with each of the cells being one LED pixel on the screen. Every cell will possess two key properties that contribute to determine the entire pattern: the cell type, which is analogous to the color of the LED; and the cell size, which is analogous to the light intensity of the LED. Therefore, a collection of cells with the same cell type (color) could represent the rough profile of an origami face, while the sizes of the cells are used to determine the face borders.. Creases and vertices can be subsequently determined by attaining the precise borders among faces. In this paper, we will also propose a crease restoration algorithm to determine the face borders and creases that then enable a fold to the final origami shape. The novel pixelated multicellular representation of origami enables new computational origami design methods as well as new self-folding origami structures.
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Ibrahim, Nagwa Ibrahim, and Manahil Mohammed Baher Edin Omer. "The Effect of Wavelength of Light on Solar Electrical Performance." In ASME 2020 Power Conference collocated with the 2020 International Conference on Nuclear Engineering. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/power2020-16096.

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Abstract The photovoltaic effect takes place at the junction of two semiconducting materials. The relation between energy (E) of light (photons) and wavelength (lambda) is given the energy of the incident photons is inversely proportional to their wavelengths. Violet is the Short-wavelength radiation, occupy the end of the electromagnetic spectrum which includes ultraviolet radiation and gamma rays. On the other hand, long-wavelength radiation occupies the red end and includes infrared radiation, microwaves, and radio waves. The wavelengths of visible light occur between 400 and 700 nm, so the bandwidth wavelength for silicon solar cells is in the very near-infrared range. Any radiation with a longer wavelength, such as microwaves and radio waves, lacks the energy to produce, electricity from a solar cell. The cost-efficiency of photovoltaic solar panels maybe reducing by reflection losses is a major field of study in the solar glass market. The color from glass cover is an important factor for the performance of photovoltaic panels as it can turn out to be an active component in the design of PV panels. Indeed, different glass covers perform very differently under direct and diffuse radiance. Several factors poignant the parameters of the solar cells, wherever these factors influence the performance on the solar cells. An experiment was carried out to investigate current interdependence on each color’s wavelength, and to give the effect regarding color cover, what part of the light of spectrum would produce a maximum power out, and also the effect of changing the wavelength (color) on short circuit current, and open voltage circuit. The results show the smallest value of maximum power in the violet zone and the biggest value of maximum power in the red zone.
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Paiè, Petra, Roberto Memeo, Federico Sala, Michele Castriotta, Thomas Vaccari, Andrea Bassi, Roberto Osellame, and Francesca Bragheri. "Dual-color on-chip light sheet microscopy of drosophila embryos." In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, edited by Daniel L. Farkas, James F. Leary, and Attila Tarnok. SPIE, 2020. http://dx.doi.org/10.1117/12.2544873.

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Burguete, Arturo, Maksim Makarenko, Fedor Getman, and Andrea Fratalocchi. "Refractive index and thickness mapping of cells from color photographs." In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIX, edited by James F. Leary, Attila Tarnok, and Irene Georgakoudi. SPIE, 2021. http://dx.doi.org/10.1117/12.2582743.

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Saito Nogueira, Marcelo, Vanderlei Salvador Bagnato, and Vitor Hugo Panhóca. "Characterization of teeth fluorescence properties due to coffee pigmentation: towards optimization of quantitative light-induced fluorescence for tooth color assessment." In Optical Interactions with Tissue and Cells XXXI, edited by Bennett L. Ibey and Norbert Linz. SPIE, 2020. http://dx.doi.org/10.1117/12.2546835.

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Tanaka, Satomi, and Norimichi Tsumura. "Improvement of analyzing method for human skin color separation by independent component analysis." In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVII, edited by Daniel L. Farkas, James F. Leary, and Attila Tarnok. SPIE, 2019. http://dx.doi.org/10.1117/12.2507672.

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Dutton, T. W., L. R. Pate, and D. K. Hollingsworth. "Liquid Crystal Imaging of Surface-Tension-Driven Convection." In ASME 2000 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2000. http://dx.doi.org/10.1115/imece2000-1430.

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Abstract Surface tension forces can drive fluid motion within thin liquid layers with a free surface. Spatial variations in the temperature of the free surface create surface tractions that drive cellular motions. The cells are most commonly hexagonal in shape and they scale on the thickness of the fluid layer. This investigation documents the formation of cells in the liquid film in the presence of a uniform-heat-flux lower boundary condition. Liquid crystal thermography was used to image the cells and measure the temperature distribution on the lower surface of the liquid layer. A 1.1-mm deep pool of silicone oil was supported on a 50-μm-thick electrically heated metal foil. The oil was retained inside an independently heated acrylic ring mounted on the top surface of the foil, and a dry-ice cooling plate served as the low-temperature sink above the free surface of the oil. Color images of hexagonal convection cells were captured using liquid crystal thermography and a digital image acquisition and processing system. The temperature distribution inside a typical cell was measured using thermographic image analysis. Experimental issues such as the use of an independently heated retaining ring to control the height of the liquid film, and the utility of a flux-based Marangoni number are discussed.
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Wang, Xufeng, Weihui Fan, and Jianyong Liao. "256x256 cell color ACPDP." In Photonics China '96, edited by Eric G. Lean, Zhiren Tian, and Bao Gang Wu. SPIE, 1996. http://dx.doi.org/10.1117/12.253355.

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Xie, Yan, Dengfeng Lu, and Jingjun Yu. "Bimaterial Micro-Structured Annulus With Zero Thermal Expansion Coefficient." In ASME 2017 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/detc2017-68142.

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This paper mainly concentrates on the design and analysis of the annulus with zero thermal expansion coefficient (ZTE) aiming to solve the heat generation and deformation in high speed bearing. First, a fork-like lattice cell inspired by the basic triangular cell is put forward and further applied to construct an annulus. The stretch-dominated lattice cell utilizes the Poisson’s contraction effect to achieve the tailorable thermal expansion coefficient (CTE). The thermal behaviors differences between the continuous interfaces and lattice cells will lead to the internal stress. Thus, the CTE of the annulus consisting of the lattice cell can be tailored to zero even negative values through the offset between the thermal-strain and force-strain. Then a theoretical model is established with some appropriate assumptions to reveal the quantitative relations among the geometrical parameters, material properties and equivalent CTEs thoroughly. The prerequisites for realizing a zero CTE are further derived in terms of material limitations and geometric constraints. Finally, FEA method is implemented to verify and analyze the thermal behaviors of annulus. The proposed annulus design characterized by the CTE tunability, structure efficiency and continuous interfaces is hopefully to be applied in the high speed bearings, adapters between the shaft and collar and fastener screws.
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Abrol, Vipasha, Sabrina Dhalla, Jasleen Saini, Ajay Mittal, Sukhwinder Singh, and Savita Gupta. "Automated Segmentation of Leukocytes using Marker-based Watershed Algorithm from Blood Smear Images." In International Conference on Women Researchers in Electronics and Computing. AIJR Publisher, 2021. http://dx.doi.org/10.21467/proceedings.114.9.

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The aim of this paper is to perform segmentation of white blood cells (WBCs) using blood smear images with the help of image processing techniques. Traditionally, the process of morphological analysis of cells is performed by a medical expert. This process is quite tedious and time consuming. The equipments used to perform the experiments are very costly and might not be available in all hospitals. Further, the whole process is quite lengthy and prone to error easily because of the lack of standard set of procedure. Hence there is a need for innovative and efficient techniques. An automated image segmentation system can make the blood test process much easier and faster. Segmentation of a nucleus image is one of the most critical tasks in a leukemia diagnosis. In this work, we have investigated and implemented image processing algorithms to segment cells. The proposed model detects WBCs and converts cell images from RGB to HSV color space using Otsu thresholding. The resultant image is then processed with the morphological filter because the segmented image contains noise which affects the system performance. Lastly, the Marker-based watershed algorithm is implemented in which specific marker positions are defined. The proposed model is tested on publically available ALL-IDB2 dataset. The system’s performance was overall examined and resulted in 98.99% overall precision for WBC segmentation.
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Reports on the topic "Colar cells"

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Sadot, Einat, Christopher Staiger, and Zvi Kam Weizmann. functional genomic screen for new plant cytoskeletal proteins and the determination of their role in actin mediated functions and guard cells regulation. United States Department of Agriculture, January 2003. http://dx.doi.org/10.32747/2003.7587725.bard.

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The original objectives of the approved proposal were: 1. To construct a YFP fused Arabidopsis cDNA library in a mammalian expression vector. 2. To infect the library into a host fibroblast cell line and to screen for new cytoskeletal associated proteins using an automated microscope. 3. Isolate the new genes. 4. Characterize their role in plants. The project was approved as a feasibility study to allow proof of concept that would entail building the YFP library and picking up a couple of positive clones using the fluorescent screen. We report here on the construction of the YFP library, the development of the automatic microscope, the establishment of the screen and the isolation of positive clones that are plant cDNAs encoding cytoskeleton associated proteins. The rational underling a screen of plant library in fibroblasts is based on the high conservation of the cytoskeleton building blocks, actin and tubulin, between the two kingdoms (80-90% homology at the level of amino acids sequence). In addition, several publications demonstrated the recognition of mammalian cytoskeleton by plant cytoskeletal binding proteins and vice versa. The major achievements described here are: 1. The development of an automated microscope equipped with fast laser auto-focusing for high magnification and a software controlling 6 dimensions; X, Y position, auto focus, time, color, and the distribution and density of the fields acquired. This system is essential for the high throughput screen. 2. The construction of an extremely competent YFP library efficiently cloned (tens of thousands of clones collected, no empty vectors detected) with all inserts oriented 5't03'. These parameters render it well representative of the whole transcriptome and efficient in "in-frame" fusion to YFP. 3. The strategy developed for the screen allowing the isolation of individual positive cDNA clones following three rounds of microscopic scans. The major conclusion accomplished from the work described here is that the concept of using mammalian host cells for fishing new plant cytoskeletal proteins is feasible and that screening system developed is complete for addressing one of the major bottlenecks of the plant cytoskeleton field: the need for high throughput identification of functionally active cytoskeletal proteins. The new identified plant cytoskeletal proteins isolated in the pilot screen and additional new proteins which will be isolated in a comprehensive screen will shed light on cytoskeletal mediated processes playing a major role in cellular activities such as cell division, morphogenesis, and functioning such as chloroplast positioning, pollen tube and root hair elongation and the movement of guard cells. Therefore, in the long run the screen described here has clear agricultural implications.
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Paran, Ilan, and Allen Van Deynze. Regulation of pepper fruit color, chloroplasts development and their importance in fruit quality. United States Department of Agriculture, January 2014. http://dx.doi.org/10.32747/2014.7598173.bard.

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Pepper exhibits large natural variation in chlorophyll content in the immature fruit. To dissect the genetic and molecular basis of this variation, we conducted QTL mapping for chlorophyll content in a cross between light and dark green-fruited parents, PI 152225 and 1154. Two major QTLs, pc1 and pc10, that control chlorophyll content by modulation of chloroplast compartment size in a fruit-specific manner were detected in chromosomes 1 and 10, respectively. The pepper homolog of GOLDEN2- LIKE transcription factor (CaGLK2) was found as underlying pc10, similar to its effect on tomato fruit chloroplast development. A candidate gene for pc1was found as controlling chlorophyll content in pepper by the modulation of chloroplast size and number. Fine mapping of pc1 aided by bulked DNA and RNA-seq analyses enabled the identification of a zinc finger transcription factor LOL1 (LSD-One-Like 1) as a candidate gene underlying pc1. LOL1 is a positive regulator of oxidative stress- induced cell death in Arabidopsis. However, over expression of the rice ortholog resulted in an increase of chlorophyll content. Interestingly, CaAPRR2 that is linked to the QTL and was found to affect immature pepper fruit color in a previous study, did not have a significant effect on chlorophyll content in the present study. Verification of the candidate's function was done by generating CRISPR/Cas9 knockout mutants of the orthologues tomato gene, while its knockout experiment in pepper by genome editing is under progress. Phenotypic similarity as a consequence of disrupting the transcription factor in both pepper and tomato indicated its functional conservation in controlling chlorophyll content in the Solanaceae. A limited sequence diversity study indicated that null mutations in CaLOL1 and its putative interactorCaMIP1 are present in C. chinensebut not in C. annuum. Combinations of mutations in CaLOL1, CaMIP1, CaGLK2 and CaAPRR2 are required for the creation of the extreme variation in chlorophyll content in Capsicum.
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Chou, Roger, Jesse Wagner, Azrah Y. Ahmed, Ian Blazina, Erika Brodt, David I. Buckley, Tamara P. Cheney, et al. Treatments for Acute Pain: A Systematic Review. Agency for Healthcare Research and Quality (AHRQ), December 2020. http://dx.doi.org/10.23970/ahrqepccer240.

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Objectives. To evaluate the effectiveness and comparative effectiveness of opioid, nonopioid pharmacologic, and nonpharmacologic therapy in patients with specific types of acute pain, including effects on pain, function, quality of life, adverse events, and long-term use of opioids. Data sources. Electronic databases (Ovid® MEDLINE®, PsycINFO®, Embase®, the Cochrane Central Register of Controlled Trials, and the Cochrane Database of Systematic Reviews) to August 2020, reference lists, and a Federal Register notice. Review methods. Using predefined criteria and dual review, we selected randomized controlled trials (RCTs) of outpatient therapies for eight acute pain conditions: low back pain, neck pain, other musculoskeletal pain, neuropathic pain, postoperative pain following discharge, dental pain (surgical or nonsurgical), pain due to kidney stones, and pain due to sickle cell disease. Meta-analyses were conducted on pharmacologic therapy for dental pain and kidney stone pain, and likelihood of repeat or rescue medication use and adverse events. The magnitude of effects was classified as small, moderate, or large using previously defined criteria, and strength of evidence was assessed. Results. One hundred eighty-three RCTs on the comparative effectiveness of therapies for acute pain were included. Opioid therapy was probably less effective than nonsteroidal anti-inflammatory drugs (NSAIDs) for surgical dental pain and kidney stones, and might be similarly effective as NSAIDs for low back pain. Opioids and NSAIDs were more effective than acetaminophen for surgical dental pain, but opioids were less effective than acetaminophen for kidney stone pain. For postoperative pain, opioids were associated with increased likelihood of repeat or rescue analgesic use, but effects on pain intensity were inconsistent. Being prescribed an opioid for acute low back pain or postoperative pain was associated with increased likelihood of use of opioids at long-term followup versus not being prescribed, based on observational studies. Heat therapy was probably effective for acute low back pain, spinal manipulation might be effective for acute back pain with radiculopathy, acupressure might be effective for acute musculoskeletal pain, an opioid might be effective for acute neuropathic pain, massage might be effective for some types of postoperative pain, and a cervical collar or exercise might be effective for acute neck pain with radiculopathy. Most studies had methodological limitations. Effect sizes were primarily small to moderate for pain, the most commonly evaluated outcome. Opioids were associated with increased risk of short-term adverse events versus NSAIDs or acetaminophen, including any adverse event, nausea, dizziness, and somnolence. Serious adverse events were uncommon for all interventions, but studies were not designed to assess risk of overdose, opioid use disorder, or long-term harms. Evidence on how benefits or harms varied in subgroups was lacking. Conclusions. Opioid therapy was associated with decreased or similar effectiveness as an NSAID for some acute pain conditions, but with increased risk of short-term adverse events. Evidence on nonpharmacological therapies was limited, but heat therapy, spinal manipulation, massage, acupuncture, acupressure, a cervical collar, and exercise were effective for specific acute pain conditions. Research is needed to determine the comparative effectiveness of therapies for sickle cell pain, acute neuropathic pain, neck pain, and management of postoperative pain following discharge; effects of therapies for acute pain on non-pain outcomes; effects of therapies on long-term outcomes, including long-term opioid use; and how benefits and harms of therapies vary in subgroups.
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Ruiz, Pablo, Craig Perry, Alejando Garcia, Magali Guichardot, Michael Foguer, Joseph Ingram, Michelle Prats, Carlos Pulido, Robert Shamblin, and Kevin Whelan. The Everglades National Park and Big Cypress National Preserve vegetation mapping project: Interim report—Northwest Coastal Everglades (Region 4), Everglades National Park (revised with costs). National Park Service, November 2020. http://dx.doi.org/10.36967/nrr-2279586.

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The Everglades National Park and Big Cypress National Preserve vegetation mapping project is part of the Comprehensive Everglades Restoration Plan (CERP). It is a cooperative effort between the South Florida Water Management District (SFWMD), the United States Army Corps of Engineers (USACE), and the National Park Service’s (NPS) Vegetation Mapping Inventory Program (VMI). The goal of this project is to produce a spatially and thematically accurate vegetation map of Everglades National Park and Big Cypress National Preserve prior to the completion of restoration efforts associated with CERP. This spatial product will serve as a record of baseline vegetation conditions for the purpose of: (1) documenting changes to the spatial extent, pattern, and proportion of plant communities within these two federally-managed units as they respond to hydrologic modifications resulting from the implementation of the CERP; and (2) providing vegetation and land-cover information to NPS park managers and scientists for use in park management, resource management, research, and monitoring. This mapping project covers an area of approximately 7,400 square kilometers (1.84 million acres [ac]) and consists of seven mapping regions: four regions in Everglades National Park, Regions 1–4, and three in Big Cypress National Preserve, Regions 5–7. The report focuses on the mapping effort associated with the Northwest Coastal Everglades (NWCE), Region 4 , in Everglades National Park. The NWCE encompasses a total area of 1,278 square kilometers (493.7 square miles [sq mi], or 315,955 ac) and is geographically located to the south of Big Cypress National Preserve, west of Shark River Slough (Region 1), and north of the Southwest Coastal Everglades (Region 3). Photo-interpretation was performed by superimposing a 50 × 50-meter (164 × 164-feet [ft] or 0.25 hectare [0.61 ac]) grid cell vector matrix over stereoscopic, 30 centimeters (11.8 inches) spatial resolution, color-infrared aerial imagery on a digital photogrammetric workstation. Photo-interpreters identified the dominant community in each cell by applying majority-rule algorithms, recognizing community-specific spectral signatures, and referencing an extensive ground-truth database. The dominant vegetation community within each grid cell was classified using a hierarchical classification system developed specifically for this project. Additionally, photo-interpreters categorized the absolute cover of cattail (Typha sp.) and any invasive species detected as either: Sparse (10–49%), Dominant (50–89%), or Monotypic (90–100%). A total of 178 thematic classes were used to map the NWCE. The most common vegetation classes are Mixed Mangrove Forest-Mixed and Transitional Bayhead Shrubland. These two communities accounted for about 10%, each, of the mapping area. Other notable classes include Short Sawgrass Marsh-Dense (8.1% of the map area), Mixed Graminoid Freshwater Marsh (4.7% of the map area), and Black Mangrove Forest (4.5% of the map area). The NWCE vegetation map has a thematic class accuracy of 88.4% with a lower 90th Percentile Confidence Interval of 84.5%.
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Friedmann, Michael, Charles J. Arntzen, and Hugh S. Mason. Expression of ETEC Enterotoxin in Tomato Fruit and Development of a Prototype Transgenic Tomato for Dissemination as an Oral Vaccine in Developing Countries. United States Department of Agriculture, March 2003. http://dx.doi.org/10.32747/2003.7585203.bard.

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The broad objective of the project was to develop a feasible approach to combat diarrheal disease caused by ETEC through the development of a low-cost oral immunogen in tomato fruit, expressed in the context of a prototype tomato that would answer the shortcomings of plant oral vaccines, especially in terms of produce handling and control of gene escape. Specifically, the goals for Boyce Thompson Institute (BTI) on this project were to develop transgenic tomato lines that express the enterotoxigenic E. coli (ETEC) heat-labile enterotoxin (LT) subunits A and/or B for use in oral edible vaccines, and to optimize expression and assembly of these antigens in tomato fruits.LT-B is a useful vaccine antigen against ETEC disease, since antibodies against LT-B can prevent binding and delivery of the holotoxinLT. Mutant forms of the toxic LT-A subunit that have reduced toxicity can be co-expressed and assembled with LT-Bpentamers to form mutant LT (mLT) complexes that could be used as mucosaladjuvants for other oral vaccines. Work on the project is continuing at Arizona State University, after Dr. Mason moved there in August 2002. A number of approaches were taken to ensure the expression of both subunits and bring about their assembly inside the transgenic fruits. Initially, expression was driven by the fruit-specific E-8 promoter for LT-B and the constitutive CaMV 35S promoter for LT-A(K63). While LT-B accumulated up to 7 µg per gram ripe fruit, assembled LT-K63 was only 1 µg per gram. Since promoter activities for the two genes likely differed in cell type and developmental stage specificity, the ratios of A and B subunits was not optimal for efficient assembly in all cells. In order to maximize the chance of assembly of mLT in fruit, we focused on constructs in which both genes are driven by the same promoter. These included co-expression plasmids using the 35S promoter for both, while switching to attenuated mLTs (LT-R72 and LT-G192) that have shown greater potential for oral adjuvanticity than the initial LT-K63, and thus are better candidates for a plant-derived adjuvant. Other, more novel approaches were then attempted, including several new vectors using the tomato fruit-specific E8 promoter driving expression of both LT-B and mutant LT-A, as well as a dicistronic construct for co-expression of both LT-B and mutant LT-A genes from a single promoter, and a geminivirusreplicon construct. We describe in the Appendix the results obtained in transgenic tomato lines transformed with these constructs. Overall, each contributed to enhanced expression levels, but the assembly itself of the holotoxin to high levels was not observed in the fruit tissues. The Israeli lab’s specific objective was to develop transgenic tomato lines expressing the LTholotoxin antigen bearing attributes to prevent gene escape (male sterility and orange fruit color) and to improve the dissemination of the oral vaccine (long shelf-life tomato cherry fruit or tomato processing background). Breeding lines bearing a number of attributes to prevent gene escape were developed by combining material and backcrossing either to a tomato cherry background, or two different processing backgrounds. Concomitantly, (these lines can be utilized for the creation of any future oral vaccine or other therapeutic-expressing tomato, either by crosses or transformation), the lines were crossed to the holotoxin-expressing tomatoes received from the United States, and this transgenic material was also incorporated into the backcrossing programs. To date, we have finalized the preparation of the cherry tomato material, both non-transgenic (bearing all the desired attributes), and transgenic, expressing the holotoxin. The level of expression of LT-B in the cherry fruits was comparable to the original transgenic tomatoes. Since it was not higher, this would necessitate the consumption of more fruits to reach a desired dose. A final backcross has been made for both the non-transgenic and the transgenic material in the processing lines. Auxin sprays resulted in high percentages of fruit set, but the processing genotypes gave many puffed fruits.
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Levin, Ilan, Avtar K. Handa, Avraham Lalazar, and Autar K. Mattoo. Modulating phytonutrient content in tomatoes combining engineered polyamine metabolism with photomorphogenic mutants. United States Department of Agriculture, December 2006. http://dx.doi.org/10.32747/2006.7587724.bard.

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Fruit constitutes a major component of our diet, providing fiber, vitamins, minerals, and many other phytonutrients that promote good health. Fleshy fruits, such as tomatoes, already contain high levels of several of these ingredients. Nevertheless, efforts have been invested in increasing and diversifying the content of phytonutrients, such as carotenoids and flavonoids, in tomato fruits. Increasing levels of phytonutrients, such as lycopene, is highly justified from the perspective of the lycopene extraction industry due to cost effectiveness reasons. Diversifying phytonutrients, in particular those that contribute to fruit color, could potentially provide an array of attractive colors to our diet. Our major goal was to devise a novel strategy for developing tomato fruits with enhanced levels of phytochemicals known to promote good health with special emphasis on lycopene content. A further important goal was to analyze global gene expression of selected genetic lines produced throughout this study in order is to dissect the molecular mechanisms regulating phytonutrients accumulation in the tomato fruit. To achieve these goals we proposed to: 1. combine, by classical breeding, engineered polyamine metabolism with photomorphogenic high pigment mutants in order generate tomato plant with exceptionally high levels of phytonutrients; 2. use gene transfer technology for genetic introduction of key genes that promote phytonutrient accumulation in the tomato fruit, 3. Analyze accumulation patterns of the phytonutrients in the tomato fruit during ripening; 4. Analyze global gene expression during fruit ripening in selected genotypes identified in objectives 1 and 2, and 5. Identify and analyze regulatory mechanisms of chloroplast disassembly and chromoplast formation. During the 3 years research period we have carried out most of the research activities laid out in the original proposal and our key conclusions are as follows: 1. the engineered polyamine metabolism strategy proposed by the US collaborators can not increase lycopene content either on its own or in combination with an hp mutant (hp-2ᵈᵍ); 2. The hp-2ᵈᵍ affects strongly the transcriptional profile of the tomato fruit showing a strong tendency for up- rather than down-regulation of genes, 3. Ontology assignment of these miss-regulated genes revealed a consistent up-regulation of genes related to chloroplast biogenesis and photosynthesis in hp-2ᵈᵍ mutants throughout fruit development; 4. A tendency for up-regulation was also usually observed in structural genes involved in phytonutrientbiosynthesis; however this up-regulation was not as consistent. 5. Microscopic observations revealed a significantly higher number of chloroplasts in pericarp cells of mature-green hp-2ᵈᵍ/hp-2ᵈᵍ fruits in comparison to their normal fully isogenic counterparts. 6. The relative abundance of chloroplasts could be observed from early stages of fruit development. Cumulatively these results suggest that: 1. the overproduction of secondary metabolites, characterizing hp-2ᵈᵍ/hp-2ᵈᵍ fruits, is more due to chloroplast number rather then to transcriptional activation of structural genes of the relevant metabolic pathways, and 2. The molecular trigger increasing metabolite levels in hp-2ᵈᵍ mutant fruits should be traced at early stage of fruit development.
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Sherman, Amir, Rebecca Grumet, Ron Ophir, Nurit Katzir, and Yiqun Weng. Whole genome approach for genetic analysis in cucumber: Fruit size as a test case. United States Department of Agriculture, December 2013. http://dx.doi.org/10.32747/2013.7594399.bard.

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The Cucurbitaceae family includes a broad array of economically and nutritionally important crop species that are consumed as vegetables, staple starches and desserts. Fruit of these species, and types within species, exhibit extensive diversity as evidenced by variation in size, shape, color, flavor, and others. Fruit size and shape are critical quality determinants that delineate uses and market classes and are key traits under selection in breeding programs. However, the underlying genetic bases for variation in fruit size remain to be determined. A few species the Cucurbitaceae family were sequenced during the time of this project (cucumber was already sequenced when the project started watermelon and melon sequence became available during the project) but functional genomic tools are still missing. This research program had three major goals: 1. Develop whole genome cucumber and melon SNP arrays. 2. Develop and characterize cucumber populations segregating for fruit size. 3. Combine genomic tools, segregating populations, and phenotypic characterization to identify loci associated with fruit size. As suggested by the reviewers the work concentrated mostly in cucumber and not both in cucumber and melon. In order to develop a SNP (single nucleotide polymorphism) array for cucumber, available and newly generated sequence from two cucumber cultivars with extreme differences in shape and size, pickling GY14 and Chinese long 9930, were analyzed for variation (SNPs). A large set of high quality SNPs was discovered between the two parents of the RILs population (GY14 and 9930) and used to design a custom SNP array with 35000 SNPs using Agilent technology. The array was validated using 9930, Gy14 and F1 progeny of the two parents. Several mapping populations were developed for linkage mapping of quantitative trait loci (QTL) for fruit size These includes 145 F3 families and 150 recombinant inbred line (RILs F7 or F8 (Gy14 X 9930) and third population contained 450 F2 plants from a cross between Gy14 and a wild plant from India. The main population that was used in this study is the RILs population of Gy14 X 9930. Phenotypic and morphological analyses of 9930, Gy14, and their segregating F2 and RIL progeny indicated that several, likely independent, factors influence cucumber fruit size and shape, including factors that act both pre-anthesis and post-pollination. These include: amount, rate, duration, and plane of cell division pre- and post-anthesis and orientation of cell expansion. Analysis of F2 and RIL progeny indicated that factors influencing fruit length were largely determined pre-anthesis, while fruit diameter was more strongly influenced by environment and growth factors post-anthesis. These results suggest involvement of multiple genetically segregating factors expected to map independently onto the cucumber genome. Using the SNP array and the phenotypic data two major QTLs for fruit size of cucumber were mapped in very high accuracy (around 300 Kb) with large set of markers that should facilitate identification and cloning of major genes that contribute to fruit size in cucumber. In addition, a highly accurate haplotype map of all RILS was created to allow fine mapping of other traits segregating in this population. A detailed cucumber genetic map with 6000 markers was also established (currently the most detailed genetic map of cucumber). The integration of genetics physiology and genomic approaches in this project yielded new major infrastructure tools that can be used for understanding fruit size and many other traits of importance in cucumber. The SNP array and genetic population with an ultra-fine map can be used for future breeding efforts, high resolution mapping and cloning of traits of interest that segregate in this population. The genetic map that was developed can be used for other breeding efforts in other populations. The study of fruit development that was done during this project will be important in dissecting function of genes that that contribute to the fruit size QTLs. The SNP array can be used as tool for mapping different traits in cucumber. The development of the tools and knowledge will thus promote genetic improvement of cucumber and related cucurbits.
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8

Hovav, Ran, Peggy Ozias-Akins, and Scott A. Jackson. The genetics of pod-filling in peanut under water-limiting conditions. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7597923.bard.

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Abstract:
Pod-filling, an important yield-determining stage is strongly influenced by water stress. This is particularly true for peanut (Arachishypogaea), wherein pods are developed underground and are directly affected by the water condition. Pod-filling in peanut has a significant genetic component as well, since genotypes are considerably varied in their pod-fill (PF) and seed-fill (SF) potential. The goals of this research were to: Examine the effects of genotype, irrigation, and genotype X irrigation on PF and SF. Detect global changes in mRNA and metabolites levels that accompany PF and SF. Explore the response of the duplicate peanut pod transcriptome to drought stress. Study how entire duplicated PF regulatory processes are networked within a polyploid organism. Discover locus-specific SNP markers and map pod quality traits under different environments. The research included genotypes and segregating populations from Israel and US that are varied in PF, SF and their tolerance to water deficit. Initially, an extensive field trial was conducted to investigate the effects of genotype, irrigation, and genotype X irrigation on PF and SF. Significant irrigation and genotypic effect was observed for the two main PF related traits, "seed ratio" and "dead-end ratio", demonstrating that reduction in irrigation directly influences the developing pods as a result of low water potential. Although the Irrigation × Genotype interaction was not statistically significant, one genotype (line 53) was found to be more sensitive to low irrigation treatments. Two RNAseq studies were simultaneously conducted in IL and the USA to characterize expression changes that accompany shell ("source") and seed ("sink") biogenesis in peanut. Both studies showed that SF and PF processes are very dynamic and undergo very rapid change in the accumulation of RNA, nutrients, and oil. Some genotypes differ in transcript accumulation rates, which can explain their difference in SF and PF potential; like cvHanoch that was found to be more enriched than line 53 in processes involving the generation of metabolites and energy at the beginning of seed development. Interestingly, an opposite situation was found in pericarp development, wherein rapid cell wall maturation processes were up-regulated in line 53. Although no significant effect was found for the irrigation level on seed transcriptome in general, and particularly on subgenomic assignment (that was found almost comparable to a 1:1 for A- and B- subgenomes), more specific homoeologous expression changes associated with particular biosynthesis pathways were found. For example, some significant A- and B- biases were observed in particular parts of the oil related gene expression network and several candidate genes with potential influence on oil content and SF were further examined. Substation achievement of the current program was the development and application of new SNP detection and mapping methods for peanut. Two major efforts on this direction were performed. In IL, a GBS approach was developed to map pod quality traits on Hanoch X 53 F2/F3 generations. Although the GBS approach was found to be less effective for our genetic system, it still succeeded to find significant mapping locations for several traits like testa color (linkage A10), number of seeds/pods (A5) and pod wart resistance (B7). In the USA, a SNP array was developed and applied for peanut, which is based on whole genome re-sequencing of 20 genotypes. This chip was used to map pod quality related traits in a Tifrunner x NC3033 RIL population. It was phenotyped for three years, including a new x-ray method to phenotype seed-fill and seed density. The total map size was 1229.7 cM with 1320 markers assigned. Based on this linkage map, 21 QTLs were identified for the traits 16/64 weight, kernel percentage, seed and pod weight, double pod and pod area. Collectively, this research serves as the first fundamental effort in peanut for understanding the PF and SF components, as a whole, and as influenced by the irrigation level. Results of the proposed study will also generate information and materials that will benefit peanut breeding by facilitating selection for reduced linkage drag during introgression of disease resistance traits into elite cultivars. BARD Report - Project4540 Page 2 of 10
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