Academic literature on the topic 'Coagulase-positive staphylococci'
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Journal articles on the topic "Coagulase-positive staphylococci"
Katic, Vera. "Significance of coagulase-positive staphylococci for microbiological food safety." Veterinarski glasnik 62, no. 5-6 (2008): 275–87. http://dx.doi.org/10.2298/vetgl0806275k.
Full textSleiniute, Jurate, and Jurate Siugzdaite. "Distribution of coagulase-positive staphylococci in humans and dogs." Acta Veterinaria Brno 84, no. 4 (2015): 313–20. http://dx.doi.org/10.2754/avb201584040313.
Full textCoimbra-e-Souza, V., M. A. V. P. Brito, R. C. Chamon, M. S. Laport, and M. Giambiagi-deMarval. "Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 69, no. 6 (November 2017): 1669–75. http://dx.doi.org/10.1590/1678-4162-9351.
Full textNWOSU, V. C. "Prevalence of Coagulase-Positive Staphylococcus in Market Meats in Awka." Journal of Food Protection 48, no. 7 (July 1, 1985): 603–5. http://dx.doi.org/10.4315/0362-028x-48.7.603.
Full textAslam, Nadia, Nazma Kiran, and Naima Mehdi. "Frequency of Isolation of Coagulase Negative Staphylococcus from Blood Cultures and its Antibiogram." Journal of Islamabad Medical & Dental College 10, no. 3 (September 30, 2021): 140–44. http://dx.doi.org/10.35787/jimdc.v10i3.468.
Full textRadoslava, Savić Radovanović, Zdravković Nemanja, and Velebit Branko. "Occurrence and Characterization of Enterotoxigenic Staphylococci Isolated from Soft Cheeses in Serbia." Acta Veterinaria 70, no. 2 (June 1, 2020): 238–54. http://dx.doi.org/10.2478/acve-2020-0017.
Full textBoipai, Manju, Krishna Chander Birua, N. P. Sahu, L. B. Pandey, Rameswari Beck, and Shashi Dinkar. "Microbiological and Biochemical Analysis of Methicillin-resistant Staphylococcus aureus Isolated from Patients Admitted in RIMS, Ranchi." Asian Pacific Journal of Health Sciences 7, no. 3 (August 5, 2020): 39–43. http://dx.doi.org/10.21276/apjhs.2020.7.3.10.
Full textHorváth, Brigitta, Ferenc Peles, László Bereczki, András Széll, Rita Sipos, Ágnes Erős, Csaba Lovász, and Adrienn Micsinai. "Comparison of sample preparation methods for the identification of Staphylococcus Aureus by MALDI-FOF MS." Acta Agraria Debreceniensis, no. 1 (May 23, 2019): 9–14. http://dx.doi.org/10.34101/actaagrar/1/2362.
Full textJankovic, Vesna, Vesna Djordjevic, Brankica Lakicevic, Branka Borovic, B. Velebit, and Radmila Mitrovic. "Determination of staphylococcal enterotoxins in cheese by immunoenzyme assays." Archives of Biological Sciences 64, no. 4 (2012): 1449–54. http://dx.doi.org/10.2298/abs1204449j.
Full textBENNETT, REGINALD W. "Atypical Toxigenic Staphylococcus and Non-Staphylococcus aureus Species on the Horizon? An Update†." Journal of Food Protection 59, no. 10 (October 1, 1996): 1123–26. http://dx.doi.org/10.4315/0362-028x-59.10.1123.
Full textDissertations / Theses on the topic "Coagulase-positive staphylococci"
Kaenjak, Anisa Wilkinson Brian J. "Osmoregulation in coagulase-positive and coagulase-negative staphylococci." Normal, Ill. Illinois State University, 1993. http://wwwlib.umi.com/cr/ilstu/fullcit?p9416856.
Full textTitle from title page screen, viewed March 6, 2006. Dissertation Committee: Brian J. Wilkinson (chair), Herman E. Brockman, H. Tak Cheung, Radheshyam K. Jayaswal, Robert L. Preston. Includes bibliographical references (leaves 162-176) and abstract. Also available in print.
Neskovic, Anika. "Characterization of Coagulase Positive Staphylococci from Pig Carcasses from Swedish Slaughterhouses." Thesis, Uppsala University, Department of Medical Biochemistry and Microbiology, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-9176.
Full textThe aim was to characterize 100 coagulase positive staphylococci isolates originating from pig carcasses from Swedish slaughterhouses by biotyping, antibiotic susceptibility testing, typing with pulsed field gel electrophoresis (PFGE) and real-time PCR-screening of the enterotoxin genes sea, sec, seg and sei in order to evaluate the impact on human health. The biotyping classified 56 as non host specific (NHS), 29 as human biotype, five as poultry, one as ovine, one as bovine biotype and eight were unclassified (UCF). Susceptibility testing to 16 antibiotics revealed that 49% of the isolates were resistant to penicillin, which the biotype human dominated among these isolates. The results from the PFGE showed correlation between the biotypes and the pulsotypes obtained with several groups with identical strains. The results from the 47 isolates tested for enterotoxins were that the combination of seg and sei was the most common but sea and sec were also detected. There were slaughterhouses that had certain biotypes and penicillin resistance linked to them.
Raupelytė, Eglė. "Koaguliazei teigiamų stafilokokų išskyrimas iš gyvūnų augintinių." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140305_133815-68093.
Full textThe The goal of the study: to determine prevalence of coagulase positive staphylococci in companion animals. The aim of the study: 1. to isolate coagulase positive staphylococci in nasal cavity of companion animals; 2. to isolate coagulase positive staphylococci in rectum of companion animals; 3. to identificate the isolated strains of staphylococci; 4. to evaluate risk factors for prevalence of staphylococci; 5. to determine antibiotic resistance in isolated staphylococci. The master study consists of 50 pages. It includes 6 tables and 14 pictures. The master study consist of 4 major chapters. The first chapter is dedicated to review of literature that is related with analized topic. This part includes coagulase positive staphylococci virulence factors, antibiotic resistance, diseases caused by staphylococci and treatment use. Furthermore chapter contains review of the prevalence and risk factors influenced the prevalence of Staphylococcus aureus and Staphylococcus pseudintermedius. The second chapter introduce with materials and methods, that were used in the research at this master study. In the third chapter the results of the research are presented. The results are presented according to the statistical reliability. The fourth chapter is the resemblance and similarity comparision of the literature review and master study research. In this master study Staphylococcus aureus and Staphylococcus pseudintermedius were isolated from nasal cavity and rectum of companion... [to full text]
Couto, Natacha. "Nasal carriage of methicillin-resistant coagulase-positive staphylococci among cats and dogs hospitalized in the Veterinary Teaching Hospital of the Faculty of Veterinary Medicine – Technical University of Lisbon, Portugal." Bachelor's thesis, Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária, 2010. http://hdl.handle.net/10400.5/2177.
Full textMethicillin-resistant coagulase-positive staphylococci (MRCPS) colonization in companion animals is an emerging and significant problem in public and animal health. During one year, nasal swabs were obtained from 40 cats and 146 dogs admitted to the Teaching Hospital of the Faculty of Veterinary Medicine - Technical University of Lisbon. MRCPS colonization was screened by plating enrichment cultures on a selective medium, Chrom MRSA ID. Bacterial species and mecA were confirmed by PCR. Clonality of the isolates was assessed by pulsed-field gel electrophoresis (PFGE). All isolates were subjected to spa and SCCmec typing. They were also tested by PCR for the lukF/lukS genes encoding Panton-Valentine leukocidin (PVL) in Staphylococcus aureus and Luk-I and Staphylococcus intermedius exfoliative toxin (SIET) in Staphylococcus pseudintermedius. Methicillin-resistant S. aureus (MRSA) was found in two cats (5 %) and one dog (0.6 %). Isolates were spa type t032, SCCmec IV and shared identical PFGE profiles. These were similar to the EMRSA-15 human clone. Strains were PVL-negative. Nine dogs carried methicillin-resistant S. pseudintermedius (MRSP) (6 %), whereas none of the cats was positive. The PFGE type A strain (n=1) showed identical characteristics as the American MRSP clone strains (CC68-MRSP-V), while PFGE cluster B grouped European MRSP isolates (CC71-MRSP-III) (n=8). All isolates were SIETnegative. The 8 European MRSP isolates were positive for the lukF/lukS genes and the American MRSP isolate was negative for both genes. Strains were multidrug-resistant, which represents a major challenge for veterinarians in terms of antibiotic therapy.
RESUMO - Frequência de colonização por staphylococci coagulase-positivo meticilinaresistente em cães e gatos internados no hospital escolar da Faculdade de Medicina Veterinária – Universidade Técnica de Lisboa, Portugal - A colonização por staphylococci coagulase-positivo meticilina-resistente (MRCPS) é um problema emergente e de grande importância em termos de saúde animal e pública. Durante um ano, zaragatoas nasais de 146 cães e 40 de gatos foram obtidas de animais internados no Hospital Escolar da Faculdade de Medicina Veterinária - Universidade Técnica de Lisboa. A colonização por MRCPSfoi pesquisada por inoculação de culturas de enriquecimento num meio selectivo, Chrom MRSA ID. As espécies de MRCPS e a amplificação do gene mecA por feita por PCR. A clonalidade dos isolados foi confirmada por PFGE. Todos os isolados foram sujeitos a tipagem spa e SCCmec. Os isolados de S. aureus meticilina-resistente (MRSA) e de S. pseudintermedius meticilina-resistente (MRSP) foram testados por PCR para a presença dos genes lukF/lukS que codificam, respectivamente, a leucocidina Panton-Valentine (PVL) e a leucocidina-I (Luk-I). Os isolados de MRSP foram ainda testados para a presença da toxina exfoliativa do S. intermedius (SIET). Nesta amostra, 0,6 % (n=1) dos cães testados e 5 % (n=2) dos gatos apresentaram MRSA. Os isolados de MRSA eram spa tipo t032, SCCmec IV e partilhavam padrões idênticos de PFGE. As estirpes eram idênticas ao clone humano EMRSA-15. Os 3 isolados eram PVL negativos. Nove cães apresentaram MRSP (6 %), enquanto nenhum dos gatos foi positivo. PFGE tipo A mostrou características idênticas ao clone americano de MRSP (CC68-MRSP-V) e PFGE tipo B agrupou os isolados europeus de MRSP (CC71-MRSP-III) (n=8). Os isolados de MRSP PFGE tipo B eram Luk-I positivos mas SIET negativos. Todas as estirpes de MRSP eram multirresistentes a várias classes de antibióticos, o que representa um desafio para os médicos veterinários em termos de estratégias de antibioterapia.
Ferreira, Jeane dos Santos. "Investigação de estafilococos coagulase positiva resistentes à meticilina em manipuladores de alimentos em hospitais públicos do município de Salvador- BA." Faculdade de Farmácia, 2012. http://repositorio.ufba.br/ri/handle/ri/27011.
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RESUMO O objetivo desse estudo foi avaliar o nível de conhecimento, atitudes e práticas dos manipuladores de alimentos em segurança alimentar; investigar a presença de estafilococos coagulase positiva resistentes à meticilina em mãos e em fossas nasais dos manipuladores; e avaliar o uso de antissépticos no controle da contaminação. O estudo desenvolveu-se em duas etapas: a primeira, através de entrevistas com o uso de um questionário auto-aplicável com 237 manipuladores e a segunda, através investigação da presença de estafilococos coagulase positiva resistentes à meticilina nas mãos e nas fossas nasais de 140 manipuladores. Este estudo está estruturado em três capítulos: o primeiro apresenta uma revisão bibliográfica com os assuntos pertinentes à pesquisa, o segundo a avaliação do nível de conhecimento, atitudes e práticas dos manipuladores em segurança alimentar e o terceiro a investigação da presença de estafilococos coagulase positiva resistentes a meticilina nas mãos e fossas nasais dos manipuladores e a avaliação in vitro para verificar a susceptibilidade das cepas isoladas aos antissépticos: álcool gel 70%, iodóforo 10% e clorexidina 2%. Os resultados demonstraram baixo nível de conhecimento para a maioria dos participantes (65,8%), apesar de 92,8% afirmarem que participaram de treinamento anterior. Em relação às atitudes e as práticas, o percentual de acertos foram de 98,3% e 73,4%, respectivamente. Foram desenvolvidos três modelos com as variáveis independentes onde se observou que a escolaridade interferiu no nível de conhecimento dos manipuladores de alimentos, mas não o treinamento. Na investigação de estafilococos coagulase positiva resistentes a meticilina nas mãos e fossas nasais, embora 100% dos manipuladores afirmassem que higienizavam suas mãos nas etapas do preparo e distribuição dos alimentos, encontrou-se 50% de amostras positivas para a presença do microrganismo, e destas 28,6% foram positivas para a presença de estafilococos coagulase positiva resistentes à meticilina (MRSA). Os isolados de MRSA apresentaram maior sensibilidade a clorexidina 2% em comparação com os outros antissépticos. Assim, pode-se concluir que o nível de conhecimento dos manipuladores em segurança alimentar é deficitário, sendo necessário reavaliar as formas de treinamento dos manipuladores de alimentos. Ainda, o uso dos antissépticos na lavagem das mãos com clorexidina 2% poderá evitar a contaminação das dietas hospitalares. Entretanto, apenas o uso de bons antissépticos não resolverá o problema se essa prática for negligenciada pelos manipuladores, ou os recursos necessários para condução da mesma não forem oferecidos pelos hospitais.
ABSTRACT The aim of this study was to evaluate the knowledge level, attitudes and practices of food handlers; investigate the presence of methicillin-resistant coagulase positive staphylococci in the hands and nares of handlers and to evaluate the use of antiseptics in the control of contamination. The study was carried out in two steps: first, through interviews with the use of a self-administered questionnaire with 237 handlers, and the second step, to investigate the presence of methicillinresistant coagulase positive staphylococci in the hands and in the nares of 140 handlers. This study is structured into three chapters: The first presents a literature review with matters relevant to the research, the second, an article on assessing the level of knowledge, attitudes and practices of food handlers and the third, an article investigating the presence of methicillin-resistant coagulase positive staphylococci (MRSA) in the hands and nares of food handlers and also the in vitro tests to check the sensitivity of these strains to antiseptics, alcohol gel 70%, iodine 10% and chlorhexidine 2%, commonly used for hand hygiene. The results showed low level of knowledge for most participants (65.8%), although 92.8% said they participated in training. Regarding the attitudes and practices, the percentage of correct answers were 98.3% and 73.4%, respectively. Three models were developed with the independent variables and were observed that education level interfered with the knowledge of food handlers. In the investigating of the presence of MRSA in the hands and nares, although 100% of the foodhandlers had informed that they sanitized their hands on the preparation and distribution of foods, we found 50% of the samples with coagulase positive staphylococci and 28.6% with MRSA. The MRSA strains were more sensitive to chlorhexidine 2% in comparison with other antiseptics. Thus, it can be concluded that the level of knowledge of food handlers in food safety is low, so it is necessary to reevaluate the ways of training of food handlers. In other side, the use of antiseptics in hand washing is essential to avoid contamination of hospital preparations. However, only the use of good antiseptics will not be sufficient if this practice is neglected by the foodhandlers, or if the hospitals do not provide the resources needed to conduct the practice.
Gatti, Júnior Pedro [UNESP]. "Qualidade higiênica e sanitária de tilápias provenientes de cultivo, comercializadas no varejo." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/86684.
Full textFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O objetivo deste trabalho foi avaliar os riscos para a saúde pública apresentado por tilápias, produzidas em cativeiro, comercializadas em supermercados na região nordeste de São Paulo. Para tal, análises microbiológicas foram realizadas, em 40 amostras de tilápias in natura e em 50 filés de tilápias. No peixe in natura foi analisado a água de enxaguadura da pele e o trato gastrintestinal. Os números mais prováveis de coliformes totais e termotolerantes foram determinados assim como a contagem Staphylococcus coagulase positivo e a presença de Salmonella spp. de acordo com a American Public Health Association (APHA). Os resultados obtidos foram analisados utilizando valores logarítmicos e as médias comparadas pelo teste de Tukey, com nível de 5% de probabilidade. A pele foi o tecido com maior nível de contaminação em relação ao trato gastrintestinal e músculo de tilápia in natura. O número de amostras com presença de coliformes totais e termotolerantes na água de enxaguadura da pele foi, respectivamente, 72,5% e 60%, no trato gastrintestinal 25% e 12,5% e no músculo 17,5% e 5%. A carga bacteriana foi significativamente maior no filé em relação ao músculo. Em duas amostras de filé foram verificados Staphylococcus coagulase positivo, uma acima dos parâmetros microbiológicos estabelecidos pela Resolução RDC nº 12/2001 da ANVISA. Salmonella spp. não foi detectada em nenhuma amostra analisada. As amostras de peixes estavam em boas condições para consumo, com exceção de duas amostras de filé. Os filés apresentaram maior contaminação que o músculo da tilápia in natura. Indicadores de poluição fecal demonstraram que a pele foi o órgão com maior contaminação
The objective of this work was to evaluate the risks to public health of consumption of tilápia fish growed in captivity and commercialised in supermarkets in the Northwest region of São Paulo state. In order to accomplish with the objective the researcher undertook analysis in 40 samples of fresh tilápias and in 50 fillets of the fish. In the fresh fish it was also analysed the washing water of the skin and of the gastrointestinal tract. It were determined, in accordance to the American Public Health Association (APHA), the number of total and thermotolerant fecal coliforms as well as the number of coagulase-positive Staphylococcus and presence of the bacteria Salmonella spp. The results were analyzed using logarithmic values and means compared by Tukey test with 5% of probability. The skin was the tissue with higher levels of contamination comparing with the gastrointestinal tract and muscle of the fresh tilapia. The percentage of samples with total and thermotolerant fecal coliforms in the skin washing water was 72.5% and 60% respectively; 25% and 12.5% in the gastrointestinal tract and 17.5% and 5% in the muscles. Bacterial presence was significantly higher in fillets comparing to muscles. Moreover, it was noticed presence of coagulase-positive Staphylococcus in two samples of fillet; one of them showed levels above of the microbiological parameters established by the RDC resolution no. 12/2001 ANVISA. Additionally, it was not identified presence of Salmonella spp in any of the samples analyzed. Generally, the studied samples were in good condition for human consumption, except for two samples of fillets. Fillets presented higher levels of contamination comparing with muscles of the fresh tilapia. Fecal pollution indicators have proved that the skin was the organ with higher levels of contamination
Muftah, Hana H. B. "Identification and antimicrobial resistance of Staphylococcus aureus from bovine milk." The Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1316462989.
Full textFarias, Marcia Fernandes de. "Ciclo reprodutivo, distribuiÃÃo populacional e condiÃÃes microbiologicas de Tagelus plebeius (Lighfoot, 1786) (MOLLUSCA: BIVALVIA: SOLECURTIDAE) no estuÃrio do rio cearà em Fortaleza - Ce." Universidade Federal do CearÃ, 2008. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=2695.
Full textOs moluscos bivalves sÃo alimentos de grande importÃncia nutricional sendo ususalmente consumidos nas regiÃes costeiras. Quando ingeridos in natura (crus) ou levemente cozidos, podem oferecer risco à saÃde pÃblica por serem filtradores e acumuladores de resÃduos quÃmicos e biolÃgicos, principalmente se a qualidade sanitÃria da Ãgua onde forem capturados estiverem comprometidas. A presente pesquisa tem como objetivo estudar a espÃcie Tagellus plebeius ("unha de velho") no estuÃrio do rio cearà em Fortaleza - Ce, considerando aspectos de seu ciclo reprodutivo, da sua biologia populacional e de suas condiÃÃes microbiolÃgicas. Para a anÃlise do ciclo reprodutivo os exemplares de T. plebeius foram coletados mensalmente no estuÃrio do rio cearà em Fortaleza - Ce (03, 42, 09.8 S; 35, 35, 49.0 W) no perÃodo de abril de 2006 a junho de 2007 em marÃs diurnas de sizÃgia. A cada mÃs as gÃnadas de 30 indivÃduos fram submetidas a exame histolÃgico. Para as amostragens microbiolÃgicas, as anÃlises foram feitas em dois meses da estaÃÃo seca )novembro e dezembro de 2006) e em dois meses da estaÃÃo chuvosa (marÃo e abril de 2007). Foram isoladas 22 cepas suspeitas de Salmonella spp., das quais 11 cepas foram confirmadas atravÃs do teste de sorologia com o antisoro polivalente O:H, sendo identificados 3 sorovares (S. Bredeney, S. London e S. Muenchen) e 1 (uma) cepa foi classificada atà subespÃcie: S. enterica subesp. enterica. Dentre os fatores ambientais observados neste estudo, a precipitaÃÃo pluviomÃtrica e a salinidade foram os que apresentaram as maiores amplitutes de variaÃÃo. O aumento da contaminaÃÃo por coliformes termotelorantes (45ÂC) na estaÃÃo chuvosa pode estar relacionado ao aporte de Ãgua doce no rio, como uma consequente diminuiÃÃo da salinidade associada a uma maior contaminaÃÃo bacteriologica. A correlaÃÃo significativa observada entre a salinidade e as fases "MaturaÃÃo/EliminaÃÃo Inicial" (M/E) e "Atresia Acular" (AC) mostra tambÃm a influÃncia desta variÃvel ambiental no desenvolvimento gonadal da espÃcie, prolongando ou reduzindo as fases do seu ciclo reprodutivo.
Bivalve molluscs are of great nutritional food ususalmente being consumed in the coastal regions. When ingested in nature (raw) or lightly cooked, can provide public health risk because filtrating and accumulators of chemical and biological waste, especially if the sanitary quality of water they are caught compromised. This research aims to study the species Tagellus plebeius ( "nail the old") on the estuary of the Rio Cearà in Fortaleza - Ce, considering aspects of their reproductive cycle, its population biology and their microbiological conditions. For the analysis of the reproductive cycle the specimens of T. plebeius were collected monthly in the estuary of the Rio Cearà in Fortaleza - Ce (03, 42, 09.8 S, 35, 35, 49.0 W) from April 2006 to June 2007 in the diurnal tides sizÃgia. Each month the gonads of 30 individuals subjected to histological examination FRAM. For microbiological samples, the tests were made in two months of dry season) in November and December 2006) and in two months of the rainy season (March and April 2007). We isolated 22 strains of suspected Salmonella spp., Of which 11 strains were confirmed by serology test with polyvalent antiserum O: H, and identified 3 serovars (S. Bredeney, S. London and S. Muenchen) and 1 (one ) strain was classified by subspecies: S. enterica subsp. enterica. Among the environmental factors observed in this study, rainfall and salinity were those who had the greatest amplitutes change. The increase of contamination by coliform termotelorantes (45 C) in the rainy season may be related to the intake of fresh water in the river, and a consequent decrease in salinity with increased bacterial contamination. The significant correlation observed between salinity and stages "Maturation / Initial Elimination" (M / E) and "incite atresia (CA) also shows the influence of the environmental variable in the gonadal development of the species, extending or reducing the phases of its cycle reproductive.
Gaspar, Carlos Alexandre Vieira. "Avaliação de perigos microbiológicos em produtos da pesca importados : Posto de Inspeção Fronteiriça do porto de Lisboa." Master's thesis, Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária, 2013. http://hdl.handle.net/10400.5/5967.
Full textO presente trabalho foi realizado no âmbito do estágio de Mestrado Integrado em Medicina Veterinária da FMV-UTL, realizado no Posto de Inspeção Fronteiriço de Lisboa da Direção-Geral de Alimentação e Veterinária. Teve como objetivo prático verificar a ocorrência de Salmonella spp. e Listeria monocytogenes e realizar a contagem de Staphylococcus coagulase positiva e E. coli em produtos da pesca proveniente de países terceiros no período de setembro de 2010 a março de 2011. Das 30 amostras analisadas (crustáceos, moluscos bivalves e delícias do mar), todas apresentaram resultados negativos na pesquisa em 25 g, relativamente a Salmonella spp. e L. monocytogenes. No que diz respeito à contagem de Staphylococcus coagulase positivos e E. coli os resultados apresentados foram, respetivamente, inferiores a 100 ufc/g de produto e a 1 ufc/g de produto, valores considerados seguros no momento da ingestão dos alimentos, de acordo com os atuais critérios microbiológicos. Com base nos resultados obtidos conclui-se que as amostras analisadas apresentaram baixos níveis de ocorrência de Salmonella spp. e L. monocytogenes e de Staphylococcus coagulase positivos e E. coli. Ainda assim, é importante a vigilância da qualidade dos produtos da pesca, a exigência constante de implementação de boas práticas e a aplicação de sistema pró-ativos de segurança dos alimentos na cadeia dos produtos da pesca, na captura e produção em aquicultura de produtos da pesca, na comercialização, nas indústrias, bem como nos procedimentos de transporte e de receção nos postos de inspeção fronteiriços.
ABSTRACT - EVALUATION OF MICROBIOLOGIC HAZARDS IN IMPORTED FISHING PRODUCTS - BORDER INSPECTION POST OF LISBOA PORT - The present work was developed for the internship of the integrated Master’s in Veterinarian Medicine, of the FMV-UTL, and carried out at the Lisbon Border Inspection Post, of the Portuguese National Authority for Animal Health (Direção-Geral de Alimentação e Veterinária). Its objective was to verify the occurrence of Salmonella spp., Listeria monocytogenes and to perform the enumeration of coagulase positive Staphylococcus and E. coli in fish coming from third countries, from September 2010 to March 2011. All 30 analyzed samples (crustaceans, bivalve molluscs and crab sticks) presented negative results for Salmonella spp. and L. monocytogenes, in 25 g. Regarding the counting of Coagulase positive Staphylococcus and E. coli, results showed less than 100 cfu/g of product and less than 1 cfu/g of product, respectively, values which, according to the current microbiological criteria, are considered safe. On the basis of the results obtained, it is concluded that the analyzed samples presented low levels of occurrence of Salmonella spp. and L. monocytogenes, as well as Coagulase positive Staphylococcus and E. coli. Even so, it is important to monitor the quality of the fishing products, and to continue to promote the implementation of good practices, and of pro-active food safety systems in the fish chain, in the capture and production of fishing products for and in aquaculture, in the commercialization, in the industries, and also in the transportation procedures and product reception at the border inspection posts.
Lima, Ariosvana Fernandes. "Staphylocuccus coagulase positiva e enterotoxinas em queijo coalho." reponame:Repositório Institucional da UFC, 2005. http://www.repositorio.ufc.br/handle/riufc/17244.
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It was evaluated the incidence of Staphylococcus sp. and Staphylococcus positivecoagulase in 80 samples of industrialized and artisanal “coalho” cheese commercialized in several selling points in Fortaleza-Ceará, as well the detection of staphylococcal enterotoxins in “coalho” cheese samples and in the isolated strains of Staphylococcus positive-coagulase. From the 80 samples of “coalho” cheese analysed, 44 (55%) were artisanal produced, being 33 (41,25%) samples of Ceará and 11 (13,75%) from other states; 13 (16,25%) industrialized with State Inspection Service (SIE) register and 23 (28,75%) industrialized with Federal Inspection Service (SIF) register. The incidence of Staphylococcus sp. in the analyzed samples of artisanal “coalho” cheese from Ceará, from other states and in the samples of industrialized “coalho” cheese with SIE, was of 100%; except for one unique (4,35%) industrialized “coalho” cheese sample with SIF register, which didn’t presented count of Staphylococcus sp. The incidence of samples with Staphylococcus sp. occurred in: 33 Ceará artisanal samples, varying from 6,0x104 to 8,9x107 UFC/g; in 11 samples of artisanal cheese from other states, varying from de 2,2 x 105 a 8,9 x 107 UFC/g; in 13 samples of industrialized cheese with SIE, varying from 9,0 x 104 a 4,3 x 107 UFC/g. These results showed a significative percentage (95,65%) of Staphylococcus sp. To the industrialized cheese. However, the contamination levels of Staphylococcus positive-coagulase in the analyzed “coalho” cheese were of: 23 (53,49%) artisanal Ceará cheese samples, with counts from 1,2 x 105 to 5,9 x 107 UFC/g; 7 (16,28%) artisanal samples from other states with counts from 6,9 x 105 to 2,6 x 107 UFC/g; 9 (20,93%) samples to the industrialized SIE register cheeses, with counts from 4,4 x 104 to 1,7 x 107 UFC/g; 4 (9,30%) samples to the industrialized “coalho” with SIF with counts from 4,7 x 105 to 2,7 x 106 UFC/g. From the 43 samples of “coalho” cheese contaminated with Staphylococcus positive-coagulase, the majority (21 samples) presented counts from 1 x 106 to 1 x 107 UFC/g. The high incidence of Staphylococcus positive-coagulase puts the consumer’s health in risk. For the staphylococcal enterotoxins analysis, there were obtained 43 extracts from the “coalho” cheese contaminated samples and 12 extracts of selected strain pools of Staphylococcus positive-coagulase, to be submitted to the detection of staphylococcal enterotoxins by the ELFA method in the VIDAS® system of bioMérieux. Inspite the high counts of Staphylococcus positive-coagulase, no staphylococcal enterotoxins were detected in any of the 43 samples of industrialized and artisanal “coalho” cheese. From the 12 strains pools of Staphylococcus positivecoagulase submitted to the detection of enterotoxins, only one strain pool (8,33%) was able to produce staphylococcal enterotoxins. These results indicate the need to orient the producers concerning to the raw material’s quality, suitable hygienic conditions and manipulation in the manufacturing of artisanal cheese; and a greater attention and fiscalization by the responsable organs, in order to practice the implementation of more rigid actions in the processing of this product, including Good Manufacturing Practices in the whole production chain for the guaranty of the products’ quality offered to the consumer and for the maintenance of the public health.
Avaliou-se a incidência de Staphylococcus sp. e Staphylococcus coagulase positiva em 80 amostras de queijos de coalho artesanal e industrial, comercializados em diversos pontos de venda em Fortaleza-Ceará, bem como a detecção de enterotoxinas estafilocócicas em queijos de coalho e nas cepas isoladas de Staphylococcus coagulase positiva. Das 80 amostras de queijos de coalho analisadas, 44 (55%) foram de produção artesanal, sendo 33 (41,25%) amostras do Ceará e 11 (13,75%) de outros estados; 13 (16,25%) industrializados com registro do SIE e 23 (28,75%) industrializados com registro do SIF. A incidência de Staphylococcus sp. nas amostras analisadas de queijos de coalho artesanais do Ceará, de outros estados e nas amostras de queijos de coalho industriais com registro do SIE, foi de 100%; com exceção de apenas uma (4,35%) amostra de queijo de coalho industrializado com registro do SIF que não apresentou contagem de Staphylococcus sp. A incidência de amostras com Staphylococcus sp. Ocorreu em: 33 amostras artesanais do Ceará, variando de 6,0 x 104 UFC/g a 8,9 x 107 UFC/g; em 11 amostras de queijos artesanais de outros estados, variando de 2,2 x 105 a 8,9 x 107 UFC/g; em 13 amostras de queijos industriais com SIE, variando de 2,2 x 105 a 1,9 x 107 UFC/g; em 22 amostras de queijos industriais com SIF, variando de 9,0 x 104 a 4,3 x 107 UFC/g. Esses resultados mostram uma porcentagem significativa (95,65%) de Staphylococcus sp. para os queijos industrializados. No entanto, os níveis de contaminação para Staphylococcus coagulase positiva nos queijos de coalho analisados foram de: 23 (53,49%) amostras artesanais do Ceará, com contagens entre 1,2 x 105 a 5,9 x 107 UFC/g; 7 (16,28%) amostras artesanais de outros estados com contagens entre 6,9 x 105 a 2,6 x 107 UFC/g; 9 (20,93%) amostras para queijos industriais com SIE, com contagens entre 4,4 x 104 a 1,7 x 107 UFC/g; 4 (9,30%) amostras para queijos de coalho industriais com SIF com contagens entre 4,7 x 105 a 2,7 x 106 UFC/g. Das 43 amostras de queijos de coalho contaminados com Staphylococcus coagulase positiva, a maioria (21 amostras) apresentou contagem entre 1 x 106 a 1 x 107 UFC/g. A alta incidência de Staphylococcus coagulase positiva colocam em risco a saúde do consumidor. Para as análises de enterotoxinas estafilocócicas, foram obtidos 43 extratos das amostras de queijos de coalho contaminadas e 12 extratos de “pools” de cepas selecionadas de Staphylococcus coagulase positiva, para serem submetidas à detecção de enterotoxinas estafilocócicas pelo método ELFA no sistema VIDAS® da bioMérieux. Não foram detectadas enterotoxinas estafilocócicas em nenhuma das 43 amostras de queijos de coalho artesanal e industrial, apesar das elevadas contagens de Staphylococcus coagulase positiva. Dos 12 pools de cepas de Staphylococcus coagulase positiva submetidos à detecção de enterotoxinas, somente um pool (8,33%) de cepas foi capaz de produzir enterotoxinas estafilocócicas. Esses resultados evidenciam a necessidade de orientação aos produtores com relação à qualidade da matéria-prima, condições adequadas de higiene e manipulação na fabricação de queijo artesanal; e uma maior atenção e fiscalização por parte dos órgãos responsáveis, no sentido de implementar medidas mais rígidas de controle no processamento desse produto, incluindo Boas Práticas de Fabricação em toda a cadeia de produção para a garantia da qualidade dos produtos oferecidos ao consumidor e para manutenção da saúde pública.
Books on the topic "Coagulase-positive staphylococci"
Federation, International Dairy. Milk and milk based products: Detection of thermonuclease produced by coagulase-positive staphylococci in milk and milk-based products. Brussels: IDF, 1987.
Find full textDawson, Susan. Other bacterial diseasesStaphylococcal zoonoses. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0026.
Full textBook chapters on the topic "Coagulase-positive staphylococci"
Chajęcka-Wierzchowska, Wioleta, and Anna Zadernowska. "Antibiotic Resistance of Coagulase-Positive and Coagulase-Negative Staphylococci Isolated From Food." In Foodborne Pathogens and Antibiotic Resistance, 349–63. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781119139188.ch15.
Full textMejia, Francisco Medina, and Stephen R. Kandall. "Coagulase-negative staphylococcal sepsis in drug-dependent newborns who may be HIV-positive." In Acquired Immunodeficiency Syndrome, 121–22. Boston, MA: Springer US, 1989. http://dx.doi.org/10.1007/978-1-4613-0807-2_33.
Full textBradley, Suzanne F. "Staphylococcus." In Schlossberg's Clinical Infectious Disease, edited by Cheston B. Cunha, 992–96. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780190888367.003.0149.
Full textKaplan, Sheldon L., Kristina G. Hulten, and Edward O. Mason. "STAPHYLOCOCCUS AUREUS INFECTIONS (COAGULASE-POSITIVE STAPHYLOCOCCI)." In Feigin and Cherry's Textbook of Pediatric Infectious Diseases, 1197–213. Elsevier, 2009. http://dx.doi.org/10.1016/b978-1-4160-4044-6.50096-0.
Full textMarsilio, Fulvio, Cristina E. Di Francesco, and Barbara Di Martino. "Coagulase-Positive and Coagulase-Negative Staphylococci Animal Diseases." In Pet-To-Man Travelling Staphylococci, 43–50. Elsevier, 2018. http://dx.doi.org/10.1016/b978-0-12-813547-1.00004-2.
Full textHota, Bala, Kyle J. Popovich, and Robert A. Weinstein. "Staphylococci." In Oxford Textbook of Medicine, 693–709. Oxford University Press, 2010. http://dx.doi.org/10.1093/med/9780199204854.003.070604_update_006.
Full textFontana, Carla, and Marco Favaro. "Coagulase-Positive and Coagulase-Negative Staphylococci in Human Disease." In Pet-To-Man Travelling Staphylococci, 25–42. Elsevier, 2018. http://dx.doi.org/10.1016/b978-0-12-813547-1.00003-0.
Full textPopovich, Kyle J., Robert A. Weinstein, and Bala Hota. "Staphylococci." In Oxford Textbook of Medicine, edited by Christopher P. Conlon, 991–1010. Oxford University Press, 2020. http://dx.doi.org/10.1093/med/9780198746690.003.0108.
Full textHumphreys, Hilary. "Case 23." In Oxford Case Histories in Infectious Diseases and Microbiology, edited by Maheshi Ramasamy, 151–57. Oxford University Press, 2020. http://dx.doi.org/10.1093/med/9780198846482.003.0023.
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