Academic literature on the topic 'CO2-BMP'
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Journal articles on the topic "CO2-BMP"
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Pabón Pereira, C. P., G. Castañares, and J. B. van Lier. "An OxiTop® protocol for screening plant material for its biochemical methane potential (BMP)." Water Science and Technology 66, no. 7 (October 1, 2012): 1416–23. http://dx.doi.org/10.2166/wst.2012.305.
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A protocol was developed for determining the biochemical methane potential (BMP) of plant material using the OxiTop® system. NaOH pellets for CO2 absorption and different pretreatment methods were tested for their influence in the BMP test. The use of NaOH pellets in the headspace of the bottle negatively affected the stability of the test increasing the pH and inhibiting methanization. Sample comminution increased the biodegradability of plant samples. Our results clearly indicate the importance of test conditions during the assessment of anaerobic biodegradability of plant material, considering BMP differences as high as 44% were found. Guidelines and recommendations are given for screening plant material suitable for anaerobic digestion using the OxiTop® system.
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Lallement, Audrey, Aline Siaud, Christine Peyrelasse, Prasad Kaparaju, Blandine Schraauwers, Samuel Maunas, and Florian Monlau. "Impact of Operational Factors, Inoculum Origin, and Feedstock Preservation on the Biochemical Methane Potential." Bioengineering 8, no. 11 (November 5, 2021): 176. http://dx.doi.org/10.3390/bioengineering8110176.
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Anaerobic digestion for the valorization of organic wastes into biogas is gaining worldwide interest. Nonetheless, the sizing of the biogas plant units require knowledge of the quantity of feedstock, and their associated methane potentials, estimated widely by Biochemical Methane Potential (BMP) tests. Discrepancies exist among laboratories due to variability of protocols adopted and operational factors used. The aim of this study is to verify the influence of some operational factors (e.g., analysis frequency, trace elements and vitamins solution addition and flushing gas), feedstock conservation and the source of inoculum on BMP. Among the operational parameters tested on cellulose degradation, only the type of gas used for flushing headspace of BMP assays had shown a significant influence on methane yields from cellulose. Methane yields of 344 ± 6 NL CH4 kg−1 VS and 321 ± 10 NL CH4 kg−1 VS obtained from assays flushed with pure N2 and N2/CO2 (60/40 v/v). The origin of inoculum (fed in co-digestion) only significantly affected the methane yields for straw, 253 ± 3 and 333 ± 3 NL CH4 kg−1 VS. Finally, freezing/thawing cycle effect depended of the substrate (tested on biowaste, manure, straw and WWTP sludge) with a possible effect of water content substrate.
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La Rosa, I., R. Fernandez-Martin, D. A. Paz, and D. F. Salamone. "106 EFFECTS OF BONE MORPHOGENETIC PROTEIN 4 (BMP4) AND ITS INHIBITOR NOGGIN ON BOVINE IN VITRO EMBRYO DEVELOPMENT." Reproduction, Fertility and Development 23, no. 1 (2011): 158. http://dx.doi.org/10.1071/rdv23n1ab106.
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Bone morphogenetic protein 4 (BMP4) is a member of the BMP family of conserved morphogenes in charge of many events of differentiation (Chen et al. 2004 Growth Factors 22, 233–241) BMP4 is involved in regulation of pluripotency in humans and mice though the role in bovine early embryo development is still undefined. Noggin is a BMP4 inhibitor (Groppe et al. 2002 Nature 420, 636–642) that does not have a specific receptor but functions by directly binding BMP ligands. The objective of this work was to study the effects of BMP4 and Noggin on early bovine embryo development. Cumulus–oocyte complexes (COC) were aspirated from abattoir ovaries and in vitro matured in TCM containing 10% fetal bovine serum (FBS), 2 mM FSH, 20 mM cysteamine, 1% antibiotic- antimycotic (15240, GIBCO, Grand Island, NY, USA) and 0.1 mM sodium pyruvate. Incubation conditions were a 6.5% CO2 humidified atmosphere at 39°C. After 22 h, in vitro fertilization was performed. Briefly, frozen–thawed semen was centrifuged twice at 490 × g and resuspended in B.O. solution to a final concentration of 20 × 106 mL–1 and incubation with COC was performed for 5 h. Presumptive zygotes were randomly cultured in CR2 with 0.3% BSA, free of serum and co-culture (control, n = 217) or supplemented with 100 ng mL–1 of either BMP4 (n = 218) or Noggin (n = 205). Cleavage and blastocyst rates were evaluated at Days 2 and 9 of culture. Blastocysts cell numbers were analysed by nuclear staining with Hoechst 33342. The expression pattern of the transcription factor Oct-4 was studied by immunocytochemistry and confocal microscope analysis in blastocysts. Chi-square tests were applied for cleavage, blastocyst, and hatching rates. One-way ANOVA was used to compare blastocyst cell number and a proportion test was used for Oct-4 expression. For all, P < 0.05 was considered significant. Cleavage rate was significantly lower in the Noggin group compared to control (51.2% v. 62.3%) whereas the BMP group (61.3%) did not differ from control or Noggin groups. Blastocyst rates for the BMP and Noggin groups were statistically lower than control (9.24% and 11.7% v. 20.6%, respectively). Hatching rate for the control group was significantly higher than both BMP and Noggin groups (4.6% v. 1.4% and 0.49%, respectively). Blastocyst cell number did not differ between groups (130, 117, and 128 for control, BMP4, and Noggin groups, respectively). Oct-4 expressing cells over total cell number was lower in BMP (72%; n = 3) and Noggin (72%; n = 3) groups compared to control (83%; n = 3). In our conditions, BMP inhibition with Noggin or addition of exogenous BMP4 negatively affected developmental rates and altered the proportion of pluripotent (Oct-4 positive) cells. Our results demonstrate the importance of a correct balance within the BMP signalling system for proper bovine in vitro embryo development.
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Krenzer, Joseph, Alyson Nelson, Trisha Robakowski, Kevin Grant, Kornelia Galior, and Sarah A. Hackenmueller. "Lipemic Interference in Basic Metabolic Panels: Increasing the Lipemia Index Threshold in Order to Decrease the Frequency of Ultracentrifugation." American Journal of Clinical Pathology 154, Supplement_1 (October 2020): S15. http://dx.doi.org/10.1093/ajcp/aqaa137.027.
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Abstract Introduction Lipemia in clinical chemistry samples is a problematic form of interference. Clearing these samples for routine testing can be time consuming and increases the turn-around time for these specimens. In our laboratory, samples with a lipemia index >50 (L-index) are manually inspected and visibly lipemic specimens are cleared by ultracentrifugation. Objective The objective of this study was to determine at what L-index ultracentrifugation of lipemic BMP specimens is necessary prior to sample testing to ensure accurate results. Methods Specimens consisted of routinely ordered basic metabolic panels (BMP) that met current criteria for ultracentrifugation, which included an L-index >50 as measured on the Abbott Architect c8000 and visual lipemia. Specimens meeting these criteria were ultracentrifuged and retested. The difference of the pre-ultracentrifuged and post-ultracentrifuged result was evaluated and put into a percent to find the ‘percent difference’ and evaluated against the total allowable error (TEa) for each analyte. If the difference observed following ultracentrifugation was less than or equal to 50% of the TEa, clearance of lipemia by ultracentrifugation was considered unnecessary. Values from all BMP component tests were analyzed in order to find an L-index threshold at which samples need to be ultracentrifuged which could be applied to the entire panel. The report of lipemic indices for BMPs for the month of January 2020 were extracted from the laboratory information system to evaluate the potential impact of altering the L-index threshold for ultracentrifugation. Results Based on the acceptance criteria of ≤50% of TEa, L-index thresholds for Na, K, Cl, calcium, glucose, creatinine, CO2 and BUN were <203, <410, <287, <387, <410, <285, < 153 and <285, respectively. All the calculated differences or percent differences for each analyte did not exceed 50% of the TEa for a given analyte when the L-index was 150 or less. Adjusting the L-index to 150 and applying it to the 195 lipemic BMP samples in January 2020, would have potentially decreased the number of samples requiring ultracentrifugation to 24 lipemic BMPs (88% reduction). Conclusion These data suggest that an L-index greater than 150 can be used for all analytes within a BMP as the threshold for requiring ultracentrifugation. The BMP is one of the most frequently ordered tests in our laboratory and consistently accounts for a substantial portion of the lipemic samples that require ultracentrifugation. Increasing the L-index at which samples will be ultracentrifuged from 50 to 150 would potentially result in an 88% reduction in one month of BMP samples requiring ultracentrifugation.
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Sloan, Andrew, Issam Hussain, Mohamed El-Sheemy, Mohammad Maqsood, Latif Mubasher, Vikas Sharma, and Oleg Eremin. "In Vitro effect of Bone Morphogenetic Protein-2 (BMP-2) and Cigarette Smoke Extract (CSE) on Osteoblastic Mesenchymal Stem Cells: Beneficial Biological Effects of BMP-2 Negated By CSE." Journal of Bone Biology and Osteoporosis 4, no. 1 (December 20, 2018): 110–20. http://dx.doi.org/10.18314/jbo.v4i1.1387.
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Introduction: Clinical and demographic studies have shown that tobacco smoking is a major contributor to non- and delayed-union in fracture healing. The cellular and molecular basis for this is poorly understood, and few studies in human fractures have been undertaken.Aims: To analyse the in vitro biological effects of tobacco smoking at the cellular level within the human fracture microenvironment, with specific regard to mesenchymal stem cell (MSC) proliferation and to ascertain whether the application of bone morphogenetic factor-2 (BMP-2) could be used therapeutically to improve fracture healing.Methods: Fracture haematomas (n=10) were collected from anaesthetised, non-smoking patients who had sustained a tibial fracture, and who were undergoing surgical operative fixation. The semi-solid material was dissected and explanted into tissue culture flasks. Complete culture media was introduced, and cultures were incubated at 37oC in a humidified 5% CO2 environment. Cigarette smoke extract (CSE) was produced and infused into the cell cultures to establish an in vitro smoking environment. A control group (n=10) was set-up and left ntreated by CSE. Harvested, spindle-shaped adherent cells were characterised by immunocytochemistry. Cell populations were counted via flow cytometry to assess and compare proliferation rates between CSE-treated and untreated cell cultures. BMP-2 concentrations (10 and 100 ng/mL (an additional dose of 500 ng/mL in CSE- reated cells)) were infused into cell cultures to enhance in vitro cellular viability, which was analysed by means of the MTT assay.Results: There was a significant reduction in the rate of proliferation of osteoblastic MSCs in CSE-treated cells after 5 days of culture (p < 0.05). At a dose of 100 ng/mL, BMP-2 augmented cellular growth and improved cellular viability in cultures not treated with CSE (p < 0.0001). No significant improvement was seen in CSE-treated cell cultures.Summary: The effect of smoking on bone fracture healing appears to contribute to the inhibition of osteoblast proliferation, which may not be reversible with the therapeutic use of exogenous BMP-2. Moreover, the improvement seen in non-smokers does strengthen the case for smokers to cease using tobacco in the perioperative setting in order that such treatments are rendered more effective.
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Pacheco, Lívia Alencar, Jenniffer Tamayo-Peña, Bruna de Souza Moraes, and Telma Teixeira Franco. "Bioenergy, Electricity, Biogas Production, and Emission Reduction Using the Anaerobic Digestion of Organic Municipal Solid Waste in Campinas, One of the Largest Brazilian Cities." Processes 10, no. 12 (December 10, 2022): 2662. http://dx.doi.org/10.3390/pr10122662.
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Anaerobic digestion (AD) is an attractive process for bioenergy production and is considered to be an alternative way to reduce landfills. AD improves municipal solid waste (MSW) management, representing a profitable application of the circular economy and could reduce environmental impact. The methane (CH4) potential of four different organic fractions of MSW—paper (PFW), garden (GFW), food (FFW), and a mixture of these three (OFMSW)—via AD was used to investigate the energy potential and the economic and environmental impact of Campinas. Theoretical and experimental biochemical methane potential (BMP) and substrate biodegradability were determined using the Buswell and Müller equation and the VDI 4630 method. The Gompertz model was used to predict the kinetics of the biochemical processes. The highest experimental BMP (410.7 NmLCH4 gVS−1) and biodegradability (86.6%) were reached with OFMSW. OFMSW can avail an energetic potential of approximately 119 GWh year−1, with a biomethane production equivalent to diesel at 49.9 × 103 m3 year−1, hence, potentially curtailing the CO2 emissions of heavy-duty vehicles by almost 133 kt year−1. The electricity demand for approximately 11% of the households in Campinas could be met by the biogas produced by OFMSW, thus increasing local energy security. The replacement of fossil diesel with biomethane to fuel garbage trucks in Campinas could reduce 25% of the diesel demand.
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Ciller, I. M., U. A. Ciller, and J. R. McFarlane. "177. ANTIBODIES AGAINST BMPR-IB UNCOVERS A PARACRINE FUNCTION FOR THE RECEPTOR IN MALE MOUSE LEYDIG CELL TESTOSTERONE PRODUCTION IN VITRO." Reproduction, Fertility and Development 22, no. 9 (2010): 95. http://dx.doi.org/10.1071/srb10abs177.
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The male reproductive system is regulated by pituitary gonadotrophins and local testicular factors including the transforming growth factor-β superfamily members which up-regulate and modulate testosterone production respectively. Type I and type II bone morphogenetic protein (BMP) receptors have been identified in both steroidogenic and non-steroidogenic cells in the testis and have been reported to impact steroid production via their effect on steroidogenic enzymes. In this study we investigated if BMPR-IB had an autocrine or paracine role in testosterone production using BMPR-IB antibodies in testis tissue and Leydig cell culture in vitro. Immature (21d) and mature (60d) mice were sacrificed by CO2 asphyxiation and the testis removed, decapsulated and cultured in basal and equine chorionic gonadotrophin (eCG)-conditioned media for three hours at 32 °C in 5 % CO2, in the presence and absence of anti-BMPR-IB. Additionally, Leydig cells were Percoll purified from adult mouse testicular interstitial cells and cultured for three hours with and without anti-BMPR-IB under human chorionic gonadotrophin (hCG) stimulated or unstimulated conditions. After three hours of incubation the culture media was aspirated into labeled vials and assayed for testosterone using a radioimmunoassay. In adult testicular slice culture, treatment with anti-BMPR-IB resulted in a significant decrease in basal and eCG-stimulated testosterone production by 37 % and 41 % respectively, while having no significant effect on basal or eCG-stimulated testosterone production by the immature testis. In purified Leydig cell culture from adult male mice BMPR-IB immunization had no effect on testosterone production in basal or hCG-stimulated conditions. In conclusion, anti-BMPR-IB significantly reduced testosterone production in adult testicular slice culture but not in cell culture, demonstrating that BMP paracrine signalling from the seminiferous tubules is likely to be important in modulating testosterone production by Leydig cells. Additionally, the paracrine signalling appears to be developmentally regulated only occurring in the adult testis.
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García, E. V., M. Hamdi, A. D. Barrera, M. J. Sánchez-Calabuig, A. Gutiérrez-Adán, and D. Rizos. "83 BONE MORPHOGENETIC PROTEIN SIGNALING DURING INTERACTION OF THE BOVINE EMBRYO WITH OVIDUCTAL EPITHELIAL CELLS IN VITRO." Reproduction, Fertility and Development 29, no. 1 (2017): 149. http://dx.doi.org/10.1071/rdv29n1ab83.
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In previous studies, we have demonstrated that different signalling components of bone morphogenetic proteins (BMP) are expressed in an anatomically and temporally regulated fashion in the bovine oviduct. However, a local response of this signalling to the embryo presence has not been elucidated yet. The aim of the present study was to evaluate whether the interaction of the embryo with the oviduct can induce changes in the gene expression of BMP signalling components. For this purpose, we used an in vitro co-culture system of a bovine oviducal epithelial cell (BOEC) monolayer with pre-implantation embryos in 2 developmental time points: before and during the main phase of embryonic genome activation (EGA). Isthmus epithelial cells from post-ovulatory stage oviducts (Day 2–4) were cultured in 500 μL of SOF + 10% FCS in 4-well plates at 38.5°C, 5% CO2, 5% O2, and 90% N2. On Day 6 of culture, medium was replaced with SOF + 5% FCS, and 24 h later BOEC monolayer was cultured in the absence or presence of in vitro-produced embryos from 2- to 8-cell stage [G1 BOEC; 33–54 h post-insemination (hpi)] or from 8- to 16-cell stage (G2 BOEC; 54–98 hpi) in the same conditions. In both groups, a polyester mesh was used to define a local co-culture area, and 30 embryos per well were placed in a 6 × 5 grid over the monolayer. In addition, as control groups, embryos in both developmental stages were cultured either in SOF + 5% FCS (G1 FCS and G2 FCS) or in SOF + 3 mg mL−1 BSA (G1 BSA and G2 BSA). At 54 hpi (G1 BOEC/BSA/FCS) or 98 hpi (G2 BOEC/BSA/FCS), embryos that reached 8- or 16-cell stage, respectively, were transferred to SOF + BSA and cultured until Day 9. The mRNA expression levels of 3 BMP receptors (BMPRIA/IB/II), 2 signalling proteins (SMAD1/5), 1 inhibitor (SMAD6), and 1 target gene (ID2) were analysed by qPCR in 5 samples of BOEC cultured with or without embryos before or during EGA, and in 3 pools of 10 embryos at 8 (54 hpi), 16 (98 hpi), and blastocyst stage (Day 7–8) from all groups. Genes H2A.Z and ACTG1 were used as housekeeping genes, and statistical differences were assessed by ANOVA. The presence of the embryo, irrespective the stage, significantly reduced the expression levels of BMPRIB, BMPRII, SMAD1, SMAD6, and ID2 in BOEC. Embryos that interacted with BOEC before EGA (G1 BOEC) showed a significant increase in the relative abundance of SMAD1 at the 8-cell stage compared with controls. Moreover, embryos that interacted with BOEC during EGA (G2 BOEC) showed a significant increase in the relative abundance of BMPRIB, BMPRII, and ID2 at the 16-cell stage when compared with controls. However, no differences were observed in the mRNA expression levels of BMP signalling components in the blastocysts between groups. In conclusion, local embryo-oviduct interaction in vitro induces changes in the transcriptional levels of BMP signalling, causing a bidirectional response that reduces the expression levels of this signalling in the oviducal cells while increases them in the embryo at early stages. This suggests that BMP signalling pathway could be involved in an early cross-talk between the bovine embryo and the oviduct during first stages of development.
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Hussein, T. S., R. B. Gilchrist, and J. G. Thompson. "327 OOCYTE-SECRETED FACTORS DIRECTLY AFFECT OOCYTE DEVELOPMENTAL COMPETENCE DURING IN VITRO MATURATION OF THE BOVINE CUMULUS - OOCYTE COMPLEX." Reproduction, Fertility and Development 18, no. 2 (2006): 271. http://dx.doi.org/10.1071/rdv18n2ab327.
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Paracrine factors secreted by the oocyte (oocyte-secreted factors, OSFs) regulate a broad range of cumulus cell functions including proliferation, differentiation, and apoptosis. The capacity of oocytes to regulate their own microenvironment by OSFs may in turn contribute to oocyte developmental competence. The aim of this study was to determine if OSFs have a direct influence on bovine oocyte developmental competence during in vitro maturation (IVM). Cumulus-oocyte complexes (COCs) were obtained by aspiration of >3-mm follicles from abattoir-derived ovaries. IVM was conducted in Bovine VitroMat (Cook Australia, Eight Mile Plains, Brisbane, Australia) supplemented with 0.1 IU/mL rhFSH for 24 h under 6% CO2 in air at 38.5�C. In the first experiment, COCs were co-cultured with denuded oocytes (DOs, 5/COC in 10 �L) beginning at either 0 or 9-h of IVM. To generate the 9-h DO group, COCs were first cultured intact for 9-h and then denuded. In the second experiment, specific OSFs, recombinant bone morphogenetic protein-15 (BMP-15) and growth differentiation factor 9 (GDF-9), were prepared as partially purified supernatants of transfected 293H cells, and used as 10% v/v supplements in Bovine VitroMat. Treatments were: (1) control (no supplement), (2) BMP-15, (3) GDF-9, (4) BMP-15 and GDF-9, and (5) untransfected 293H control. Following maturation, in vitro production of embryos was performed using the Bovine Vitro system (Cook Australia) and blastocysts were examined on Day 8 for development. Developmental data were arcsine-transformed and analyzed by ANOVA, followed by Tukey's test. Cell numbers were analyzed by ANOVA. Co-culturing intact COCs with DOs from 0 or 9 h did not affect cleavage rate, but increased (P < 0.001) the proportion of cleaved embryos that reached the blastocyst stage post-insemination (50.6 � 1.9 and 61.3 � 1.9%, respectively), compared to COCs cultured alone (40.7 � 1.4%). Therefore, paracrine factors secreted by DOs increased the developmental competence of oocytes matured as COCs. OSFs also improved embryo quality, as co-culture of COCs with DOs (0 or 9 h) significantly increased total cell (156.1 � 1.3 and 159.1 � 1.3, respectively) and trophectoderm (105.7 � 1.3 and 109.8 � 0.4, respectively) numbers, compared to control COCs (total = 148 � 1.2, trophectoderm = 98.2 � 0.8, P < 0.001). BMP-15 alone or with GDF-9 also significantly (P < 0.001) increased the proportion of oocytes that reached the blastocyst stage post insemination (57.5 � 2.4% and 55.1 � 4.5%, respectively), compared to control (41.0 � 0.9%) and 293H-treated (27.1 � 3.1%) COCs. GDF-9 also increased blastocyst yield (49.5 � 3.9%) but not significantly. These results are the first to demonstrate that OSFs, and particularly BMP-15 and GDF-9, directly affect bovine oocyte developmental competence. These results have far-reaching implications for improving the efficiency of IVM in domestic species and human infertility treatment, and support the role of OSF production by oocytes as a diagnostic marker for developmental competence.
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Zeng, Shengquan, Riley Harris, and Eunsung Kan. "Effect of Alfalfa-Derived Biochar on Anaerobic Digestion of Dairy Manure." Agronomy 12, no. 4 (April 11, 2022): 911. http://dx.doi.org/10.3390/agronomy12040911.
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Biochemical methane potential (BMP) tests were conducted for investigating the effects of alfalfa-derived biochar (AF-BC) on anaerobic digestion (AD) of dairy manure under various loading of AF-BC (0–10 g/L). BMP tests were performed at mesophilic temperature (37 °C) with the addition of AF-BC. Biogas and methane volumes and concentrations, water quality parameters (i.e., COD (chemical oxygen demand)), and volatile fatty acids (VFAs) were measured during the AD process. The addition of 1 and 5 g/L of AF-BC increased the biogas yields by 15.51% and 26.09% and methane yields by 14.61% and 26.88% compared with the control without addition of AF-BC. Additionally, the addition of AF-BC (1–10 g/L) decreased the lag phase by 7.14–22.45% and the CO2 content of biogas by 13.60–32.48%, while increasing the COD removal efficiency by 19.19–35.94% in the AD of dairy manure. Moreover, the addition of AF-BC also decreased total VFAs and acetic acid concentrations in the AD process. The increase in AD performance was mainly owing to the improvement of buffering ability of the AD system and direct interspecies electron transfer (DIET) among AD microorganisms resulting from the addition of AF-BC. In contrast, the addition of 10 g/L AF-BC did not show any obvious improvement in biogas and methane yields in the AD of dairy manure, possibly because of toxic effects from excessive addition of AF-BC toward the AD microorganisms. Therefore, this study supported practical feasibility of AF-BC-enhanced AD of dairy manure.
Conference papers on the topic "CO2-BMP"
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Nwaigwe, Kevin N., Abhishek Agarwal, and Emmanuel E. Anyanwu. "Biogas Potentials Evaluation of Household Wastes in Johannesburg Metropolitan Area Using the Automatic Methane Potential Test System (AMPTS) II." In ASME 2018 12th International Conference on Energy Sustainability collocated with the ASME 2018 Power Conference and the ASME 2018 Nuclear Forum. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/es2018-7553.
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A work on biogas potentials evaluation of household wastes in Johannesburg metropolitan area using the Automatic Methane Potential Test System (AMPTS) II is presented. The AMPTS II consists of three units — the sample incubation unit, CO2 absorption unit and the gas volume measuring device. Organic fraction of wastes collected from households within Johannesburg metropolis were sorted, ground and prepared into slurry by mixing with water. Microcrystalline cellulose powder with 3.5% loss on drying and 0.28g/cc density was used as control substrate while anaerobic sludge collected from a functional biogas reactor was used as inoculum. Anaerobic sludge was classified as sample A, household waste containing mainly non-food waste was labelled sample B, sample C was microcrystalline cellulose used as positive control while household waste composing of mainly food waste was classified as sample D. Each sample was fed into a 50 mL bottle reactor in triplicates and stirred in a clockwise direction continuously for 5 minutes with a pulse interval of 1 minute at a set temperature of 37°C for 30 days retention time. NaOH solution was prepared into solution following standard procedure and mixed with a prepared 0.4 % Thymolpthalein solution. The resultant solution was poured into the 100 mL bottles of the CO2 unit. Produced biogas was measured through water displacement in the volumetric bath and values read off through a data-logger connected to a laptop. Results indicated biochemical methane potential (BMP) of 69–800 NmL/gvs and biogas composition with more than 50% methane before CO2 fixing and over 80% after CO2 fixing. Given that the average amount of waste generated per person per day in South Africa is over 0.7 kg, there is huge potentials for biogas production from household wastes in Johannesburg.