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Postulka, Aleš. "Zobrazení a úprava informací v Transparency and Consent Framework." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2021. http://www.nusl.cz/ntk/nusl-445505.
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This thesis deals with the development of multilingual for web browsers Mozilla Firefox and Google Chrome. The purpose of the extension is to enable the automated management of provided consents to the processing of personal data on websites using the Transparency and Consent Framework. Extension was developed on the basis of knowledge about this framework and about legal norms GDPR and ePrivacy Directive, which deal with the protection of personal data. Knowledge of the method of developing extensions for web browsers using WebExtensions was also used during the implementation. During testing, consent was successfully enforced in 96,2 % of tested websites in Mozilla Firefox. In Google Chrome, success has been achieved in 82,1 % of tested websites. The banner requiring consent was not displayed in 33 % of websites in Mozilla Firefox and in 31,1 % of websites in Google Chrome.
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Janßen, Julia Annika [Verfasser], Ali [Akademischer Betreuer] El-Armouche, Christopher [Akademischer Betreuer] Antos, Henning [Gutachter] Morawietz, and Ali [Gutachter] El-Armouche. "In vivo FLIM-FRET as a novel technique to assess cAMP and cGMP in the intact zebrafish heart / Julia Annika Janßen ; Gutachter: Henning Morawietz, Ali El-Armouche ; Ali El-Armouche, Christopher Antos." Dresden : Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2018. http://d-nb.info/1151816477/34.
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Perina, Fernando Cesar. "Avaliação de risco ecológico devido à contaminação por metais no setor sul do Complexo Estuarino-Lagunar Cananéia-Iguape." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/21/21134/tde-15022017-112048/.
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O histórico de contaminação por metais no Vale do Rio Ribeira de Iguape é bem conhecido, com evidências de transporte destes contaminantes ao Complexo Estuarino Lagunar de Cananéia-Iguape (CELCI), adsorvidos ao material em suspensão. Assim, os metais podem alcançar o setor sul do CELCI, podendo acumular nos sedimentos e nos organismos filtradores. Neste estudo foi realizada uma avaliação de risco ecológico no CELCI associado à presença de metais oriundos das antigas áreas de mineração do Alto Ribeira, utilizando diferentes linhas de evidência. Os resultados foram avaliados de forma integrada, incluindo análises geoquímicas e biológicas (ensaios de toxicidade aguda, medidas de bioacumulação e biomarcadores bioquímicos em brânquias de bivalves). Os sedimentos apresentaram níveis moderados a altos de metais e toxicidade aguda, a qual foi associada, especialmente, com as concentrações de Cu, Pb e Zn. Da mesma forma estes metais foram encontrados em concentrações altas nos tecidos de bivalves. Os biomarcadores bioquímicos (MT, GSH, GST, GPx, GR, AChE, LPO e danos em DNA) indicaram a ocorrência de efeitos significativos nos bivalves. Portanto, os resultados mostram que os metais estão sendo carreados para a porção sul do estuário, causando bioacumulação e efeitos nos organismos aquáticos e, portanto, gerando riscos ecológicos para o CELCI.The historic of contamination by metals in the Valley of Ribeira de Iguape River is well known, and there are evidences that these chemicals are carried to the Estuarine Complex of Cananeia-Iguape (ECCI), adsorbed on the suspended particles. Metals can thus reach the south of the ECCI, where they may accumulate in the sediments and filter feeding organisms. This study aimed to evaluate the ecological risks associated to the metals originated from the former mining areas of the upstream Ribeira de Iguape River, by using different lines of evidence, which included geochemical and biological analysis (acute toxicity tests, bioaccumulation and biochemical biomarkers in bivalves\' gills). The sediments presented moderate to high levels of metals and acute toxicity, which was correlated with the concentrations of metals (Cu, Pb and Zn). These elements were found in high concentrations in the bivalves soft tissues. The biochemical biomarkers (MT, GSH, GST, GPx, GR, AChE, LPO, and DNA damage) indicated the occurrence of significant effects in the bivalves. Therefore, the results showed that metals are carried into the South portion of the estuary, accumulating in the biota and causing negative effects to the aquatic organisms, producing thus ecological risks to the ECCI.
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Crozé, Noémie de. "Rôles des facteurs de transcription AP2⍺ et Hairy2 dans l'induction de la crête neurale : Etude chez l'embryon de xénope." Paris 11, 2009. http://www.theses.fr/2009PA112320.
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La crête neurale (CN) est une population de cellules pluripotentes migrantes spécifique des embryons de vertébrés. Sa formation est contrôlée par un réseau de régulations épistatiques conservées au cours de l’évolution impliquant les facteurs secrétés BMP, FGF et WNT. Ces facteurs induisent l’expression des déterminants de la bordure neurale qui vont, à leur tour, induire l’expression des déterminants de la crête neurale. Au cours de ma thèse, je me suis intéressée aux mécanismes d’induction de la CN et plus particulièrement au rôle joué par les facteurs de transcription AP2a et Hairy2 dans le réseau de régulations épistatiques contrôlant sa formation. Ces deux protéines sont exprimées précocement à la bordure neurale et interviennent dans le contrôle du développement de la CN. Mes travaux montrent qu’AP2a dans un premier temps joue un rôle central dans l’intégration des signaux FGF et WNT et dans l’induction de la bordure neurale. Dans un deuxième temps, il contrôle le développement de la CN indépendamment de son action sur le maintien de la bordure neurale. Ces travaux montrent aussi qu’Hairy2 intervient en aval de la voie FGF et en parallèle d’AP2a pour permettre le maintien de la bordure neurale. Il bloque la spécification des précurseurs de la CN et renforce l’expression des déterminants de la bordure neurale. Ces résultats ont permis d’enrichir le réseau de régulation épistatique contrôlant la formation de la CN, de révéler un nouveau rôle d’AP2 dans le développement de cette structure et de proposer un modèle du contrôle de l’équilibre entre le maintien d’une population de précurseurs des cellules de la crête neurale et l’initiation de leur spécificationThe neural crest is a multipotent, migratory cell population of vertebrate embryos that give rise to a variety of cell derivatives. An evolutionary-conserved epistatic network, which involves secreted factor BMP, FGF and WNT; has been proposed to control neural crest formation. This gene network suggests secreted factors induce the expression of neural border specifiers that will in turn induce neural crest specifiers genes. During my doctoral training I have investigated early steps in the induction of neural crest cells, focusing on the role of transcription factors AP2 and Hairy2. AP2 and Hairy2 are both expressed in the presumptive neural crest domain and are required for neural crest induction. My work demostrates that AP2 activity controls sequential steps in the neural crest network. First, AP2 acts as a neural border specifier to integrate FGF and WNT signals and allow neural border formation. Second, AP2 acts as a neural crest specifier in neural crest formation independent from its earlier role in neural border establishment. Additionally, I showed that Hairy2 activity is required in parallel with AP2 and downstream of FGF signaling to allow neural border formation. Hairy2 both blocks neural crest specification and maintains neural border genes expression. Hairy2 also induces Delta1 expression leading to activation of Notch pathway in the adjacent cells and suggest an additional role in specification of this tissue. Taking together my results allowed us to place AP2 and Hairy2 at specific steps in the regulatory network, which controls neural crest formation. This serves to highlight a new role of AP2 in neural crest development. Furthermore, I propose a model to explain how the embryo sets up a balance between neural crest cell specification and maintenance of a progenitor pool
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Kavčič, Silvija. "Überleben und Erinnern slowenische Häftlinge im Frauen-Konzentrationslager Ravensbrück /." Berlin : Metropol, 2007. http://catalog.hathitrust.org/api/volumes/oclc/221306493.html.
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Lecoq, Anne-Lise. "Implication d'AIP (Aryl hydrocarbon receptor Interacting Protein) dans la tumorigenèse des adénomes hypophysaires." Thesis, Université Paris-Saclay (ComUE), 2015. http://www.theses.fr/2015SACLS028.
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Nous avons souhaité, dans ce travail de Thèse, préciser l'impact de l'invalidation d'AIP sur la fonction sécrétoire et la prolifération des cellules somatotropes in vivo et explorer in vitro les voies de signalisation potentiellement impliquées dans la tumorigenèse hypophysaire AIP-dépendante. L'analyse du phénotype des souris Aip+/-, en particulier de la sécrétion pulsatile de GH, montre que, contrairement à l'Homme, les animaux mutés ne développent pas de gigantisme ni d'hypersécrétion de GH et que la pénétrance de la pathologie tumorale hypophysaire est beaucoup plus faible qu'initialement décrit. Les études réalisées sur les fibroblastes de patients mutés pour AIP et porteurs d'un adénome hypophysaire ainsi que sur les cellules somatolactotropes de rat GH3 révèlent que les mutations d'AIP altèrent l'activité transcriptionnelle d'AhR (Aryl hydrocarbon Receptor), mais affectent également la voie de signalisation de l'AMPc. Enfin, des mutations germinales du gène GPR101, récemment identifiées dans des cas d'acromégalie sporadique, ont aussi été trouvées chez des patients porteurs d'un adénome hypophysaire sporadique non somatotrope, sans association avec les mutations d'AIP. Ce travail a ainsi permis de préciser les conséquences des mutations d'AIP sur la fonction somatotrope et la signalisation d'AhR. Le rôle de l'AMPc dans la tumorigenèse hypophysaire AIP-dépendante sera évalué dans un nouveau modèle de souris transgéniqueIn this work, we investigated the effects of AIP deficiency in vivo on somatotroph cells, both at the secretory and proliferative levels and explored in vitro the signaling pathways potentially involved in AIP-dependent pituitary tumorigenesis. Phenotype analyzes of Aip+/- mice, especially of GH pulsatility, show that, unlike humans, mutant mice do not develop gigantism nor GH hypersecretion and present with a much lower penetrance of pituitary adenomas than initially described. In vitro studies in fibroblasts of AIP-mutation positive patients with pituitary adenomas and in somatolactotroph GH3 cells demonstrate that AIP mutations alter AhR (Aryl hydrocarbon Receptor) transcriptional activity and modify the cAMP pathway. Finally, GPR101 mutations, recently reported in patients with sporadic acromegaly, have also been identified in a small portion of patients with sporadic non-somatotroph pituitary adenomas, without any association with AIP mutations. This research work defines the consequences of AIP mutations on somatotroph cell function and AhR transcriptional activity. The role of cAMP signaling in AIP-related pituitary tumorigenesis will be further evaluated in a new transgenic mouse model
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Göttsch, Hans-Joachim. "Untersuchungen zu C.P. Snow's Romansequenz "Strangers and brothers"." Hamburg : [H.-J. Göttsch], 1985. http://catalog.hathitrust.org/api/volumes/oclc/38668802.html.
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Ramsden, Graziana. "Manuel Puig, Pedro Almodóvar and the politics of camp." [S.l. : s.n.], 2003. http://catalog.hathitrust.org/api/volumes/oclc/81324447.html.
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Jacinto, T?lio W?gner Batista. "Adi??o de superplastificante e anti-segregante ? base de CMC em pastas de cimento para Po?os de Petr?leo." Universidade Federal do Rio Grande do Norte, 2008. http://repositorio.ufrn.br:8080/jspui/handle/123456789/12655.
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Previous issue date: 2008-02-29Oil well cementing materials consist of slurries of Special class Portland cement dispersed in water. Admixtures can be used to provide the necessary fluidity, so the material can be efficiently pumped down as well as penetrate porous rocks with controlled filter loss. Construction admixtures can be used to modify the properties of oil well cements provided they can withstand and hold their properties at the higher than ambient temperatures usually encountered in oil fields. In civil construction, superplasticizer play the role of dispersants that reduce the facto r of water cement improve mechanical properties and fluidity of the cement, whereas anti-segregation agents improve the workability of the slurry. In the present study, oil well cement slurries were produced adding both a dispersant and an anti-segregation agent conventionally used in Portland CPII-Z-32 RS cement aiming at materials for primary cementing and squeeze operations. Three basic aspects were evaluated: fluidity, filter loss and the synergetic effect of the admixtures at two temperatures, i.e., 27?C and 56?C, following API RP 10B practical recommendations. The slurries were prepared using admixture concentrations varying from 2.60 Kgf/m3 (0.02 gallft3) to 5.82 Kgf/m3 (0.045 galJft3) BWOC. The density of the slurries was set to 1.89 g/cm3 (15.8 Ib/gal). 0.30 to 0.60% BWOC of a CMC-based anti-segregation agent was added to the cement to control the filter loss. The results showed that the addition of anti-segregation at concentrations above 0.55% by weight of cement resulted in the increased viscosity of the folders in temperatures evaluated. The increasing the temperature of the tests led to a reduction in the performance of anti-segregation. At concentrations of 5.20 kgf/m3 (0,040 gallft3) and 5.82 Kgf/m3 (0,045 gal/ft 3) observed a better performance of the properties evaluated in the proposed system. At low temperature was observed instability in the readings of rheology for all concentrations of anti-segregation. Contents that increasing the concentration of anti?-segregation is limited concentrations greater than 0.55 % BWOC of the CMC in temperature analyzed. The use of the system with CMC promoted a good performance against the properties evaluated. The principal function of anti?-segregation was optimized with increasing concentration of superplasticizer, at temperatures above the 2rC. The study of the behaviour of systemic additives, resulting in slurries of cement, which can be optimized face studies of other intrinsic properties in oil fields
A pasta de cimento Portland ? uma mistura de part?culas s?lidas de cimento dispersas em ?gua. Tal sistema deve possuir fluidez suficiente para penetrar de forma eficiente em meio poroso, al?m de proporcionar controle na ?gua perdida por filtrado na forma??o, garantindo o sucesso da opera??o de bombeio. Em determinados campos as condi??es geol?gicas das forma??es, bem como a profundidade das faces explorat?rias do po?o podem favorecer ao acr?scimo de temperatura. Na constru??o civil os superplastificantes reduzem o fator ?gua cimento das argamassas proporcionando aumento de propriedades como resist?ncia mec?nica e fluidez, j? o anti-segregante proporciona uma melhor trabalhabilidade das argamassas. Sistemas com a adi??o de dispersantes e anti?segregante utilizados convencionalmente em cimento Portland CPII-Z-32 RS foram analisados em pastas de cimento Portland Especial, utilizadas para opera??es de squeeze e cimenta??o em po?os petrol?feros, com ?nfase em tr?s aspectos: fluidez, perda do volume de filtrado e efeito sinerg?tico. Os par?metros analisados foram estudados por meio de ensaios de desempenho nas temperaturas de 2rC e 56?C, seguindo as recomenda??es pr?ticas da norma API RP 108. Para o estudo foram selecionados materiais com car?ter dispersante como superplastificantes. e preparadas pastas com concentra??es variando de 2,60 Kgf/m3 (0,02 gal/p?3) a 5,82 Kgf/m3 (0,045 gal/p?3) em peso de cimento, com densidade constante de 1,89 g/cm3 (15,8 Ib/gal). Para o controle da ?gua perdida por filtra??o foi utilizado um agente anti-segregante a base de CMC com concentra??es variando de 0,30 % a 0,60 % em peso de cimento. Os resultados mostraram que a adi??o do anti-segregante em concentra??es acima de 0,55 % em peso de cimento resultou no aumento da viscosidade das pastas nas temperaturas avaliadas. Foi verificado que o aumento da temperatura dos testes provocou uma redu??o no desempenho do anti-segregante. Nas concentra??es de 5,20 kgf/m3 (0,040 gal/p?3) e 5,82 Kgf/m3 (0,045 gal/p?3) observou-se um melhor desempenho das propriedades avaliadas no sistema proposto. Na temperatura ambiente foi verificado instabilidade nas leituras de reologia para todas as concentra??es do anti-segregante. Foi verificado que o aumento da concentra??o de anti-segregante ? limitada as concentra??es superior a 0,55 % em peso de cimento nas temperaturas analisadas. O uso do sistema contendo superplastificante, aliado ao anti-segregante promoveu um bom desempenho face ?s propriedades reol?gicas e de controle de filtrado das pastas. A fun??o principal do anti-segregante foi otimizada com o aumento da concentra??o do superplastificante, a temperaturas acima da ambiente. Por?m o aumento da concentra??o do agente dispersante nas pastas de cimento pode provocar o retardamento das pastas em temperaturas pr?ximas da ambiente. O estudo do comportamento sist?mico dos aditivos resultou na obten??o de pastas de cimento, que podem ser otimizadas face estudos de outras propriedades intr?nsecas a sua utiliza??o nas opera??es em campos petrol?feros
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DeGeer, Donna. "A novel oncogene AHI-1 interacts with BCR-ABL and JAK2 and mediates cellular resistance to tyrosine kinase inhibitors in CML." Thesis, University of British Columbia, 2010. http://hdl.handle.net/2429/24851.
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Chronic myeloid leukemia is a myeloproliferative disorder characterized by the presence of the Philadelphia chromosome, encoding a unique fusion gene BCR-ABL. The current first line treatment for patients diagnosed with CML involves administration of the ABL kinase inhibitor imatinib mesylate (IM). However, early relapses and acquired drug resistance remain a current impediment to successful treatment for many patients. This suggests the necessity for alternate treatment options which may include combination therapy targeting multiple vital proteins involved in the malignancy of the leukemia. AHI-1 (Abelson helper integration site 1) is a recently discovered oncogene that is highly deregulated in murine lymphomas and leukemias. AHI-1 displays a significant pattern of overexpression in a Philadelphia chromosome positive cell line K562 cells. To investigate AHI-1’s involvement in CML, AHI-1 was either stably overexpressed or suppressed in K562 cells. Interestingly, an increase in cellular proliferation and colony formation and a decrease in apoptosis were observed in the presence of IM when AHI-1 was overexpressed, while suppression of AHI-1 had the opposite effects. Phosphorylation and total protein expression levels of several proteins known to be involved in BCR-ABL signalling were quantified. Interestingly, elevated phosphorylation and total gene/protein expression levels of several of these proteins were observed when AHI-1 was overexpresessed, in particular NF-κB and JAK2/STAT5 displayed increased expression. Due to the strong effects AHI-1 had on the JAK2/STAT5 signalling cascade, we then inhibited JAK2 activity using a new JAK2 inhibitor, TG101209. AHI-1 overexpression led to a reduction in the cellular response to the inhibitor while suppression of AHI-1 caused an increase in sensitivity in viability, apoptosis, and colony forming cell assays. Finally, a combination of IM and TG101209 was examined in the same K562 cells lines. Results from suggest that using a combination treatment approach was more effective at inhibiting cellular viability and colony formation than either treatment alone. These findings together suggest that AHI-1 may play an important role in mediating cellular resistance to IM and TG101209 and activates several BCR-ABL signalling pathways, and that it may be a vital target in eradicating the malignant leukemic cells arising in CML.
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Sullivan, Kathryn. "Dissertation Report IS 8995 Using Dialog CIP At Winona State University To Educate End-Users." NSUWorks, 1991. http://catalog.hathitrust.org/api/volumes/oclc/27024072.html.
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Coulon, Pierre-Grégoire. "Interactions VIH/autophagie dans les cellules dendritiques : de la réplication à la présentation des antigènes." Thesis, Paris 6, 2014. http://www.theses.fr/2014PA066552/document.
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Le VIH-1 manipule les cellules présentatrices d’antigènes (APC) qui orchestrent les réponses immunes innées et adaptatrices, pour se propager dans l’hôte et établir le réservoir viral. Au laboratoire, nous étudions le rôle de l’autophagie dans les interactions entre les cellules dendritiques (DC) et le VIH-1 et la présentation des antigènes viraux. Dans divers modèles, la macroautophagie et l’autophagie médiée par les chaperonnes (CMA) semblent en effet être impliquées dans l’apprêtement d’antigènes sur les molécules du CMH. Ainsi, nous avons montré, dans une étude précédente, que la macroautophagie participait à la dégradation du VIH entrant dans les DC, conduisant à l’activation de lymphocytes T (LT) CD4+ spécifiques du VIH-1.Bien que sa réplication y soit limitée, le VIH-1 peut également infecter productivement les DC. J’ai donc voulu vérifier si les protéines virales néosynthétisées du virus peuvent constituer une source additionnelle d’antigènes. J’ai montré que, de façon remarquable, dans les DC infectées, des antigènes endogènes du VIH-1 peuvent être présentés par les molécules du CMH-II aux LT CD4 spécifiques. En utilisant différents outils, comme des inhibiteurs de l’autophagie ou des shRNA, j’ai montré que ni la macroautophagie ni la CMA ne contribuent significativement à l’apprêtement d’épitopes de la protéine virale Gag néosynthétisée sur les molécules du CMH-II. En parallèle, j’ai utilisé une protéine de fusion, Gag-LC3, pour acheminer spécifiquement Gag dans les autophagosomes (LC3+) des DC. Dans ce contexte, les drogues qui inhibent la macroautophagie réduisent drastiquement la présentation d’épitopes de Gag aux LT CD4. De façon remarquable, la présence de Gag dans les autophagosomes conduit à la génération d’épitopes antigéniques qui, dans le contexte infectieux, ne sont pas apprêtés sur les molécules de CMH-II par la voie endogène. Ainsi, diriger des protéines du VIH dans les autophagosomes conduirait à des variations dans le répertoire des antigènes endogènes présentés sur les molécules de CMH-II. Pour évaluer l’impact de l’autophagie sur la réplication du VIH dans les DC, j’ai ensuite analysé si la protéine Gag néosynthétisée pouvait être dégradée dans les autophagosomes. Dans les DC infectées, contrairement aux observations déjà décrites dans les macrophages, Gag ne colocalise ni avec les vésicules autophagiques LC3+, ni avec p62, une protéines adaptatrice impliquée dans le ciblage des protéines dans les autophagosomes. Ces résultats suggèrent que, dans ce contexte, les virions nouvellement produits ne sont pas acheminés et dégradés dans les autophagosomes. La protéine de fusion Gag-LC3 est utilisée dans ces expériences comme contrôle positif de colocalisation. Pour déterminer si mes observations pouvaient révéler un mécanisme d’échappement développé par VIH-1, j’ai utilisé différentes souches virales mutantes, modulé le flux autophagique avec des drogues et des ligands TLR, et exprimé Gag dans les DC en l’absence d’autres protéines virales. Dans l'ensemble, mon travail suggère que le VIH-1 ne manipule pas la macroautophagie dans les DC productivement infectées. En outre, la modulation de l’autophagie dans les DC (à l'aide de shRNA) n'a aucune incidence sur la réplication du VIH-1 et sur sa propagation.Mes travaux mettent en lumière la complexité des interactions entre l’autophagie et le VIH-1 dans les DC. Contrairement à ce qui a été observé lors des étapes d’entrée du virus, le virus ne semble pas être acheminé dans les autophagosomes une fois les DC infectées, et l’autophagie ne participe pas à l’apprêtement des antigènes néosynthétisés sur les molécules de CMH II. Cependant, les DC infectées activent de façon efficace les LT CD4 spécifiques du virus. Forcer l’acheminement d’antigènes du VIH dans les autophagosomes augmente fortement cette activation, et semble conduire à une diversification du répertoire des épitopes présentés sur les molécules de CMH-II par la voie endogèneHIV-1 manipulates antigen-presenting cells (APC) such as dendritic cells (DC), witch orchestrate innate and adaptive immune responses, in order to propagate in the host and to establish viral reservoirs. We are studying the role of autophagic processes in DC/HIV-1 interactions with a focus on antigen presentation. We have previously shown that macroautophagy in DC participates in the degradation of incoming HIV-1 particles leading to activation of HIV-1-specific (HS) CD4 T cells. HIV-1 can also productively infect DC. I thus first asked whether neo-synthetized viral proteins might represent an additional source of HIV-1 antigens. Remarkably, I have shown using infected monocyte derived DC that de novo expression of Gag leads to the activation of HS CD4 T cells, highlighting that this antigen is endogenously processed in order to be presented into MHC-II molecules. Since macroautophagy and chaperon-mediated autophagy (CMA) are known to be involved in this process for other viral antigens and model antigens, I then dissected the role of these two pathways. Using several tools including inhibitors and shRNA, I demonstrated that in HIV-1-infected DC neither macroautophagy nor CMA contribute significantly to the processing of HIV-1-Gag epitopes into MHC-II molecules. I also used a Gag-LC3 fusion protein to specifically channel Gag into LC3+ autophagic vesicles in DC. In this context, inhibiting autophagy dramatically reduced the presentation of HIV-1-Gag epitopes to CD4+ T cells. Strikingly, channelling Gag into autophagosomes generated epitopes that were not processed endogenously in the context of HIV-1 infection. Thus specifically directing HIV-1 proteins toward autophagosomes might influence the repertoire of MHC II-restricted HIV-1 antigens. I further analyzed whether autophagy could affect HIV-1 replication in infected DC. In these cells, in contrast to what has been described in macrophages, Gag did not colocalize with LC3 or with the autophagic adaptor p62, suggesting that newly-produced HIV-1 particles are not sequestrated into autophagosomes. The Gag-LC3 fusion protein was used here as a positive control of colocalization. To determine whether my findings might reveal a DC-specific escape mechanism developed by HIV-1, I used various HIV-1 mutants, enhanced autophagic flux using drugs or TLR ligands, and expressed Gag in the absence of other HIV-1 proteins. Overall, my work suggests that HIV-1 does not manipulate autophagy in productively-infected DC. Moreover, modulating autophagy in DC (using shRNA) does not impact HIV-1 replication and propagation. Finally, my work highlights the complexity of the interactions between the autophagic process and HIV-1 replication in DC. Unlike during viral entry, HIV-1 does not seem to be targeted into autophagosomes after viral replication in infected DC, and autophagy does not contribute significantly to the processing of endogenous viral antigens. Nonetheless HIV-1-infected DC efficiently activates HS CD4 T cells, and targeting HIV antigens into autophagosomes greatly enhances this activation and might broaden the repertoire of MHC-II-restricted antigen. Further dissection of the various routes of endogenous HIV antigen processing would aid in the development of innovative vaccines
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Pritchett, Kelly. "Perceptions and Expressions of Social Presence During Conversations on Twitter." Thesis, 2011. http://hdl.handle.net/1969.1/ETD-TAMU-2011-12-10616.
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Computer-mediated environments such as social media create new social climates that impact communication interactions in un-mediated environments. This study examined social variables during conversations on Twitter through a qualitative document analysis that coded messages into affective, interactive or cohesive categories. Perceived social presence, participant satisfaction, and relationships between social presence and satisfaction among Twitter users during streaming conversations were examined through an online questionnaire that was created using qualtrics.com and made available to respondents over a one-week period.
The researcher concluded that most social variables in the Twitter conversations of this study fall into the interactive social presence category. In addition, each category of responses functions in a different way to foster social presence. Two groups of survey respondents agreed with 10 out of 21 and 13 out of 21 statements about social presence and 10 out of 13 and 12 out of 13 statements about satisfaction. Findings indicated that positive and negative relationships exist between social presence and satisfaction.
Both conversations in this study appeared to be successful. Therefore, agricultural communicators should feel comfortable using CMC more frequently to circulate agricultural information among populations across the globe. It was recommended that further research be conducted to examine social presence among new topics, populations, and other forms of CMC.
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Gabbay, Robert Abraham. "Mechanism of insulin antagonism of cAMP-mediated hormone action." 1985. http://catalog.hathitrust.org/api/volumes/oclc/13014218.html.
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Abo-Sharkh, Basel Fathi Mohammad. "Feasibility of CMA production by alkaline fusion of cellulose." 1990. http://catalog.hathitrust.org/api/volumes/oclc/23666695.html.
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Kim, Myoung-hee. "A measure of consicuous consumption propensity CCP scale development /." 2001. http://catalog.hathitrust.org/api/volumes/oclc/50074038.html.
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Thesis (M.S.)--University of Wisconsin--Madison, 2001.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 76-83).
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Sparrow, Carl Peter. "Biosynthesis and utilization of CDP-diglyceride in Escherichia coli." 1985. http://catalog.hathitrust.org/api/volumes/oclc/12363455.html.
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Po-Hsuan and 陳博玄. "Tongkat Ali (Eurycoma longifolia) decrease the PDE5A gene expression and enhance the NO and cGMP concentration of the plasma." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/48108866942795387684.
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碩士中山醫學大學
醫學研究所
97
Eurycoma longifolia (Tongkat Ali) is a plant with folk medicine in Indonesia, Malaysia, Thailand and Vietnam. The residences in Malaysia usually treat it as “ginseng of Malaysia”, and think it possesses multiple medical functions such as anti-hypertension and appetite stimulation. The purpose of this project was to examine the effect of E. longifolia extract on plasma NO and cGMP concentration in mice. Viagra was also used for comparison. Mice were treated by E. longifolia, Viagra, L-Arginine, Tongkat Ali plus ginseng, Dioscorea for 2 and 4 weeks. Plasma NO, cGMP level and the activity of type 5 phophodiesterase and cytochrome P450 were analyzed. Results showed that 5 treatments increased the NO and cGMP levels, and inhibited the activity of type 5 phophodiesterase. Tongkat Ali and Tongkat Ali plus ginseng treated mice at 4 weeks has significantly higher cytochrome P450 mRNA expression. These treatments did not affect the anti-oxidative defense in liver and kidney. These findings implied that Tongkat Ali could provide protective or medical functions via elevating plasma NO and cGMP concentration and decreasing PDE5 enzyme activity.
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Casimir, David Alan. "Regulation of early preadipocyte differentiation cAMP and prostaglandin F₂[alpha] /." 1996. http://catalog.hathitrust.org/api/volumes/oclc/36569652.html.
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Thesis (Ph. D.)--University of Wisconsin--Madison, 1996.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (lweaves 133-145).
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Williams, Rolf S. "An actor's process Michael Frayn's Noises Off and C.P. Taylor's Good /." 1988. http://catalog.hathitrust.org/api/volumes/oclc/18390213.html.
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Thesis (M.F.A.)--University of Wisconsin--Madison, 1988.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 52-56).
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Nett, Cristabel. "Rehabilitation boot camp: an innovative, four-week program to deliver intensive balance and mobility therapy to people with acquired brain injury (ABI)." 2015. http://hdl.handle.net/1993/30975.
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Acquired Brain Injury (ABI) can cause balance and mobility deficits with activity and participation limitations. Repetitive Functional Task Practice (RFTP), currently best practice to promote recovery, is often not delivered at an adequate volume due to limited resources.
This case series looked at the feasibility of treating community-dwelling people with ABI, in a group format, thus allowing economical, intense rehabilitation. Four participants attended for four weeks, three days/week, 4.25 hours/day. One-to-one and semi-supervised therapy was delivered with one therapist and one assistant. 89.51 minutes of RFTP and 134.82 minutes of total physical therapeutic activity was delivered per day. Participant satisfaction was good.
All participants improved on some clinical measures. Three participants improved single and dual-task balance measures. This project established feasibility, allowed the formation of guiding principles for and supported the value of future research and development of this ABI Boot Camp model.February 2016
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Boxer, Matthew E. "It's not just about the money emerging adulthood and camp staff turnover /." 2005. http://catalog.hathitrust.org/api/volumes/oclc/61207335.html.
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Thesis (M.S.)--University of Wisconsin--Madison, 2005.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 52-56)
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Uǧuz, Cevdet. "The role of cyclic adenosine monophosphate (cAMP) during capacitation of bovine sperm." 1994. http://catalog.hathitrust.org/api/volumes/oclc/32919123.html.
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Thesis (M.S.)--University of Wisconsin--Madison, 1994.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 57-84).
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Loy, Martin John. "A study of the effectiveness of a camp intervention for bereaved adolescents." 1999. http://catalog.hathitrust.org/api/volumes/oclc/44410925.html.
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Thesis (Ph. D.)--University of Wisconsin--Madison, 1999.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 126-133).
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Leineweber, Daniel B. "Production of calcium magnesium acetate (CMA) from dilute aqueous solutions of acetic acid." 1992. http://catalog.hathitrust.org/api/volumes/oclc/32195163.html.
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Thesis (M.S.)--University of Wisconsin--Madison, 1992.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 162-173).
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Liu, Chen. "C axes from radar depolarization experiments at UPB Camp on ice stream B Antarctica." 1994. http://catalog.hathitrust.org/api/volumes/oclc/33058709.html.
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Thesis (M.S.)--University of Wisconsin--Madison, 1994.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 62-64).
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Knap, Jane Alice. "Use of the detective novel form by C.P. Snow, Gabriel Garcia Marquez and Vladimir Nabokov." 1989. http://catalog.hathitrust.org/api/volumes/oclc/21890653.html.
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Thesis (Ph. D.)--University of Wisconsin--Madison, 1989.Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 321-333).
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Mook, Carrie S. "Youth development at a girls' residential camp positive outcomes and growth experiences of young adult counselors /." 2005. http://catalog.hathitrust.org/api/volumes/oclc/61196483.html.
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Thesis (M.S.)--University of Wisconsin--Madison, 2005.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 99-106)
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Noel, Richard J. "The lac -45 region, CAP, and RNA polymerase three partners in transcription initiation in Escherichia coli /." 1998. http://catalog.hathitrust.org/api/volumes/oclc/41036933.html.
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Jaeckels, Jeffrey Michael. "Positive matrix factorization (PMF) of carbonaceous aerosols for source apportionment and comparison to chemical mass balance (CMB) apportionment." 2007. http://catalog.hathitrust.org/api/volumes/oclc/153260239.html.
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Gendreau, Steven B. "Characterization of the Caenorhabditis elegans Kip/Cip cyclin-dependent kinase inhibitor family members, cki-1 and cki-2." 1998. http://catalog.hathitrust.org/api/volumes/oclc/42696748.html.
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Thesis (Ph. D.)--University of Wisconsin--Madison, 1998.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 140-151).
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Duran, Ergin. "Research on registration of 3-D geometric features and an automated inspection system integrating CMM and solid modelling systems." 1996. http://catalog.hathitrust.org/api/volumes/oclc/35980246.html.
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Thesis (M.S.)--University of Wisconsin--Madison, 1996.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 121-124).
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Blake, Kevin P. "Depositional processes and glacial margins associated with the late glacial isolation and retreat of Svartisen Ice Cap, Northern Norway." 1998. http://catalog.hathitrust.org/api/volumes/oclc/42706497.html.
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Thesis (Ph. D.)--University of Wisconsin--Madison, 1998.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 124-133).
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Davis, Jinx. "Chaos and comedy creating the roles of Belinda in Noises Off by Michael Frayn and Helen in Good by C.P. Taylor /." 1988. http://catalog.hathitrust.org/api/volumes/oclc/18230019.html.
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Thesis (M.F.A)--University of Wisconsin--Madison, 1988.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 145-148).
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Muljadi, Anton. "Air-injectd clean-in-place (CIP) for milking systems Development for a study method and characterization of chemical, mechanical and thermal factors /." 1996. http://catalog.hathitrust.org/api/volumes/oclc/37328984.html.
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Thesis (M.S.)--University of Wisconsin--Madison, 1996.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 76-82).
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Jensen, Bruce Robert. "Studies of the role of intracellular cAMP in development and cell-fate and the characterization of a novel protein kinase in Dictyostelium discoideum." 1993. http://catalog.hathitrust.org/api/volumes/oclc/30841842.html.
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Thesis (Ph. D.)--University of Wisconsin--Madison, 1993.Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (144-179).
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Krusky, J. Bradley. "Survival of the indirect pulp cap a retrospective study : a thesis submitted in partial fulfillment ... for the degree of Masters' [sic] of Science in Pediatric Dentistry ... /." 2001. http://catalog.hathitrust.org/api/volumes/oclc/68944127.html.
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