Dissertations / Theses on the topic 'Clostridium botulinum'
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Sharma, Davinder Kumar. "Toxin production by Clostridium botulinum." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301991.
Full textRaffestin, Stéphanie. "Régulation de la toxinogenèse chez Clostridium botulinum et Clostridium tetani." Paris 7, 2005. http://www.theses.fr/2005PA077044.
Full textDavis, Tom Owen. "Regulation of botulinum toxin complex formation in Clostridium botulinum : type A NCTC 2916." Thesis, Open University, 1998. http://oro.open.ac.uk/57744/.
Full textCooksley, Clare Marie. "Characterisation of a putative agr system in Clostridium botulinum and Clostridium sporogenes." Thesis, University of Nottingham, 2008. http://eprints.nottingham.ac.uk/11463/.
Full textHielm, Sebastian. "Molecular detection, typing and epidemiology og Clostridium botulinum." Helsinki : University of Helsinki, 1999. http://ethesis.helsinki.fi/julkaisut/ela/elint/vk/hielm/.
Full textConnan, Chloé. "Neurotoxinogénèse et Passage des neurotoxines botuliques à travers la barrière intestinale." Thesis, Paris 11, 2013. http://www.theses.fr/2013PA114830/document.
Full textBotulinum neurotoxins (BoNTs), produced by C. botulinum, are responsible for animal and human botulism. In its natural form, botulism is mostly acquired after absorption of BoNTs in the digestive tract after ingestion of food contaminated with C. botulinum and its toxins. The intoxination can be divided in 4 major steps: toxin production, ingestion of food contaminated with BoNTs, passage of BoNTs through the intestinal barrier, and proteolytic activity on nerve endings. Regulation of toxin production and passage of BoNTs through the intestinal barrier are poorly understood. BoNT associates with non toxic protein (NAPs) to form complexes of various sizes. The BoNTs and NAPs genes are clustered in the botulinum locus and are positively regulated by an alternative sigma factor BotR/A. Toxinogenesis in C. botulinum is regulated by a complex regulatory network containing at least 3 two components systems (TCS), identified by antisens RNA strategy, which regulate the production of botulinum complex independently of BotR/A. On the other hand, BoNT/B entry was monitored with fluorescent HcB fragment in ligatureted mouse intestinal loop. Fluorescent imaging analysis, immunohistochemistry and electron microscopy, have evidence that HcB is transcytosed through enterocytes cells by an endocytosis dynamin dependant. HcB targets acetylcholinergic nerves localized in lamina propria of villi then reaches serotoninergic and acetylcholinergic nerve endings in the submucosa and musculosa within 10 minutes. In vitro experiments performed on intestinal cell line (m-ICcl2) shows that the endocytosis of HcB is dependent on the GD1b/GT1b gangliosidic receptors on the cell surface but not on the synaptotagmine II protein which is recquiered HcB entry in neuronal cells
Masuyer, Geoffrey. "Structure and activity of Clostridium botulinum neurotoxin functional fragments." Thesis, University of Bath, 2012. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.557825.
Full textWoudstra, Cedric. "Clostridium botulinum, du génotypage de la toxine en passant par les flagellines jusqu'au séquençage de génomes : un aperçu de la diversité génétique des Clostridies associés au botulisme animal et humain." Thesis, Paris Est, 2016. http://www.theses.fr/2016PESC1020/document.
Full textClostridium botulinum is the etiologic agent of botulism, a deadly paralytic disease that can affects both human and animals. Different bacteria, producing neurotoxins type A to H, are responsible for the disease. They are separated into different groups (I to VI) on the basis of their phenotypical and biological characteristics. Human botulism is mainly due to Groups I and II producing neurotoxins A, B, E and F, with type H recently discovered. Also C. butyricum and C. baratii species (Groups V and VI), producing toxins type F and E respectively, are scarcely reported. C. argentinense Group IV, producing toxin type G, which has been suspected to be associated with infant botulism in Argentina. Animal botulism is mainly due to Group III, which is constituted by C. novyi sensu lato species. They produce toxin types C, D and their mosaic variants. Botulinum neurotoxins are the most powerful toxin known to date with as little as 70 µg enough to kill a person by food poisoning. Therefore, it received a great deal of attention. Botulinum neurotoxins have been deeply studied, especially human related toxins compared to animal. The toxins found to be useful for medical or cosmetic (Botox) treatments, but it was also used as a biological warfare agent, and for bioterrorism. Its extreme potency is equal to its dangerousness. Therefore, governments show concerns of its potential misuse as a bioterrorism weapon; research programs are funded to study and raise awareness about both the toxins and the producing organisms. My PhD work was structured by the different projects I was involved in, which were related to C. botulinum detection and typing, like BIOTRACER and AniBioThreat European projects, the French national CBRN program, or the NRL for avian botulism. The main transversal objective I followed lead me to develop new methods to trace back the origin of C. botulinum contamination, in case of a deliberate, accidental or naturally occurring botulism outbreak. I investigated flagellin genes as potential genetic targets for typing C. botulinum Group I-II and III, responsible for human and animal botulism respectively. Flagellin genes flaA and flaB showed the investigated C. botulinum Group I and II strains to cluster into 5 major groups and up to 15 subgroups, some being specific for certain geographical areas, and flaB being specific to C. botulinum type E. Flagellin fliC gene investigated in C. botulinum Group III showed to cluster into five groups, with fliC-I and fliC-IV associated to type C/D and D/C respectively, being not discriminative enough to differentiate highly genetically related strains. I also studied the prevalence of mosaic toxin genes in C. botulinum Group III in animal botulism, mainly in poultry and bovine. The results brought out the mosaic toxin types C/D and D/C to be predominant in the samples investigated throughout Europe. Finally, I explored the full genome sequences of 14 types C/D and 3 types D/C C. botulinum Group III strains, mainly originating from French avian and bovine botulism outbreaks. Analyses of their genome sequences showed them to be closely related to other European strains from Group III. While studying their genetic content, I was able to point out that the extrachromosomal elements of strains type C/D could be used to generate a genetic ID card. Investigation of Crispr typing method showed to be irrelevant for type C/D, due to a deficient Crispr-Cas mechanism, but deserve more investigation for type D/C. The highest level of discrimination was achieved while using SNP core phylogeny, which allowed distinguishing up to the strain level. Here are the results I’m going to develop in this manuscript
Bradbury, Mark. "Genomic and flow cytometric studies of Clostridium sporogenes, a non-toxigenic surrogate for Clostridium botulinum." Thesis, Federation University Australia, 2014. http://researchonline.federation.edu.au/vital/access/HandleResolver/1959.17/97216.
Full textDoctor of Philosophy
Bucknavage, Martin M. "Growth and survival of Clostridium botulinum type E in pasturized oysters." Thesis, This resource online, 1988. http://scholar.lib.vt.edu/theses/available/etd-04122010-083636/.
Full textLA, TORRE ANGELA. "Studi molecolari del processo di germinazione in Clostridium sporogenes, modello non-patogeno di Clostridium botulinum." Doctoral thesis, Università Cattolica del Sacro Cuore, 2016. http://hdl.handle.net/10280/10793.
Full textWhen environmental conditions are unfavorable to the growth, Bacillus and Clostridium bacteria (including Clostridium botulinum, the causative agent of foodborne botulism) form endospores, metabolically dormant cell types resistant to several adverse conditions and difficult to kill. However, under suitable conditions, spores resume the vegetative life by triggering the germination process. Thus, spores are dangerous agents of human foodborne disease and food spoilage. In this work, the strain Clostridium sporogenes UC9000, isolated from raw milk, was used like not-pathogenic model of Clostridium botulinum to better understand the mechanisms underpinning the Clostridium germination. Clostridium sporogenes is a species phylogenetically related to Clostridium botulinum and often used like its surrogate. Physiological studies revealed that UC9000 spores germinate in presence of L-alanine/L-cysteine in combination with L-lactate, while in silico analyses allowed the identification of homologues of the Bacillus germinant receptors responsive to L-alanine. The genome screening also detected genes coding for SleB, CwlJ and SleL, enzymes participating to the cortex degradation. CwlJ was found resident in the spore coat by performing a proteomic analysis, it was expressed in soluble form in E. coli and an in vitro assay of activity revealed its capability to induce germination when added exogenously to decoated spores
LA, TORRE ANGELA. "Studi molecolari del processo di germinazione in Clostridium sporogenes, modello non-patogeno di Clostridium botulinum." Doctoral thesis, Università Cattolica del Sacro Cuore, 2016. http://hdl.handle.net/10280/10793.
Full textWhen environmental conditions are unfavorable to the growth, Bacillus and Clostridium bacteria (including Clostridium botulinum, the causative agent of foodborne botulism) form endospores, metabolically dormant cell types resistant to several adverse conditions and difficult to kill. However, under suitable conditions, spores resume the vegetative life by triggering the germination process. Thus, spores are dangerous agents of human foodborne disease and food spoilage. In this work, the strain Clostridium sporogenes UC9000, isolated from raw milk, was used like not-pathogenic model of Clostridium botulinum to better understand the mechanisms underpinning the Clostridium germination. Clostridium sporogenes is a species phylogenetically related to Clostridium botulinum and often used like its surrogate. Physiological studies revealed that UC9000 spores germinate in presence of L-alanine/L-cysteine in combination with L-lactate, while in silico analyses allowed the identification of homologues of the Bacillus germinant receptors responsive to L-alanine. The genome screening also detected genes coding for SleB, CwlJ and SleL, enzymes participating to the cortex degradation. CwlJ was found resident in the spore coat by performing a proteomic analysis, it was expressed in soluble form in E. coli and an in vitro assay of activity revealed its capability to induce germination when added exogenously to decoated spores
Preising, Claudia. "Literaturstudie zum Vermehrungs- und Toxinbildungs- vermögen von Clostridium botulinum, zu den Eigenschaften des Botulinumtoxins sowie zum Vorkommen und zur Tenazität der Clostridium botulinum-Sporen." Doctoral thesis, Universitätsbibliothek Leipzig, 2006. http://nbn-resolving.de/urn:nbn:de:swb:15-20070118-093729-8.
Full textBruhne, Lars. "Untersuchungen zur Beziehung zwischen positivem Clostridium botulinum Antikörper-Nachweis, ausgewählten Stoffwechselparametern, Akute-Phase-Proteinen und Erkrankungshäufigkeiten, Herdengröße sowie Herdenmilchleistung von Milchrindern." Doctoral thesis, Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-171930.
Full textBrown, Robert Christopher. "Development of a novel expression system in Clostridium perfringens." Thesis, Open University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321561.
Full textMarvaud, Jean-Christophe. "Contribution a l'etude des proteines associees aux neurotoxines clostridiennes et vectorisation de proteines dans les cellules (doctorat : microbiologie)." Paris 11, 1998. http://www.theses.fr/1998PA114851.
Full textCadieux, Brigitte. "Development of a novel, rapid, in vitro assay for the detection of Clostridium botulinum neurotoxin type E." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=32836.
Full textThe specificity of the antisera was increased by adsorbing cross-reactive antibodies from whole antisera with affinity columns made with total proteins from culture supernatants of closely related clostridia. Alternatively, specific antibodies were isolated with an affinity column prepared with C. botulinum type E toxoid.
Different methods of concentrating BoNT/E in each sample prior to testing them were evaluated to increase the sensitivity of the assay.
The slot blot immunoassay was then evaluated for detection of BoNT/E in mixed cultures and in food samples. (Abstract shortened by UMI.)
Alberto, François. "Etude physiologique et moléculaire de la germination des spores clostridium botulinum." Aix-Marseille 3, 2003. http://www.theses.fr/2003AIX30009.
Full textThe aim of this work is to elucidate some of the physiological and molecular mechanisms implicated in Clostridium botulinum spore germination. This strictly anaerobic bacterium is able to produce dormant spores when the environmental conditions become unfavourable. Spores can survive for a long time and are resistant to a wide range of physical and chemical treatments. Germination is defined as the conversion of a dormant spore into a metabolic active vegetative cell. Bacterial spores germinate in response to small molecules (amino acids, sugars, ions) called germinants. The first step of this work was to determine the main conditions of germination for different strains of mesophylic C. Botulinum. It was demonstrated that C. Botulinum spores germinate at 30ʿC in response to an induction by L-alanine at various germination rates according to the strain. This response is mainly enhanced by addition of L-lactate as a co-germinant. Only hydrophobic amino acids are able to trigger C. Botulinum spore germination. Spore germination is influenced dramatically by temperature. At 15ʿC, few spores germinate in L-alanine/L-lactate. The molecular analysis of the putative germinant receptors has revealed the presence of a three-gene operon in C. Botulinum Beans B, homologous to genes gerAA, gerAB and gerAC of the Bacillus subtilis gerA operon. Analysis of the gene transcription and mRNA mapping showed that expression is very high during late sporulation and controlled by a sG factor. Western-blot analysis have revealed that GerAB protein is located in inner membrane of the spore. The results obtained with C. Botulinum and the previous data on other sporulating species suggest that the first step of spore germination may be the same in all spore-forming bacteria
Blount, Benjamin Andrew. "Analysis of the roles and regulation of flagella in Clostridium botulinum." Thesis, University of Nottingham, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.588079.
Full textAshton, Philip Matthew. "A genomic and proteomic investigation into the Clostridium botulinum neurotoxin complex." Thesis, Open University, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.664471.
Full textFohler, Svenja [Verfasser]. "Occurrence of Clostridium botulinum neurotoxin genes and toxin-genotypes of Clostridium perfringens in dairy cattle / Svenja Fohler." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2016. http://d-nb.info/1104403897/34.
Full textMorineaux, Valérie. "Identification et Quantification des Sous-Types de la Neurotoxine Botulique de Type A par Spectrométrie de Masse." Thesis, Paris 11, 2015. http://www.theses.fr/2015PA114826.
Full textBotulinum neurotoxins (BoNTs) are the most poisonous substances known. They are responsible for human botulism, a rare but potentially fatal disease if not quickly treated. However, BoNTs were approved for the treatment of numerous medical applications due to their temporary paralysis effects. BoNTs are among the six agents with the highest risk of potential use as bio-weapons because of their high toxicity in aerosol form. BoNTs, produced by Clostridium botulinum, are divised into seven toxinotypes and each toxinotype contains several subtypes. This biodiversity makes more difficult their identification with classical methods by immunological ways. Until now, only molecular genetical methods could differenciate subtypes among them. The aim of this work was to develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) in MRM mode to efficiently discrimate the distinct subtypes from specific and common peptides. Immunocapture sample preparation with antipeptides antibodies was used and allowed the isolation of the toxin from the sample. Subtyping was performed with crude supernatants (BoNT/A1 to /A3, /A5, /A7 and /A8) in order to validate the method. Limit of detection (LOD) of the proposed method is in the range of minimal toxin concentration found in naturally contamined samples. In a second part of this work, this mass spectrometry method was used to quantify the neurotoxin in complex matrices (supernatants of Clostridium botulinum cultures). Isotope labeled light chain (13C6]K et [13C6]R) from botulinum A1 neurotoxin was produced and used as internal standart. Subtypes were quantified in supernatants and the quantity of neurotoxin for one minimal lethal dose 100% was determined for each subtype
Taylor, Reed H. "Conditions Associated with Clostridium sporogenes Growth as a Surrogate for Clostridium botulinum in Non-thermally Processed Canned Butter." BYU ScholarsArchive, 2010. https://scholarsarchive.byu.edu/etd/2447.
Full textRuth, Vieira de Lemos Vasconcelos Maria. "Efeito da acidificação com diferentes ácidos sobre as características sensoriais e inibição do Clostridium botulinum no palmito de pupunha em conserva." Universidade Federal de Pernambuco, 2004. https://repositorio.ufpe.br/handle/123456789/9010.
Full textA porção comestível do tronco de algumas palmeiras, utilizadas para a produção de palmito é um produto de importância para o Brasil como principal produtor e exportador na forma em coonserva, detendo 85% da produção mundial. A região Nordeste tem se destacado como produtora da variedade pupunha (Bactris gasipaes), nativa da Amazônia, que apresenta vantagens ecológicas por se tratar de palmeira cultivada, com características de perfilhamento, precocidade de produção e qualidade satisfatória. A ocorrência de toxinfecções por Clostridium botulinum provocadas pelo consumo de palmito industrializado levou o Ministério da Saúde a estabelecer a obrigatoriedade do uso de ácido associado à salmoura para produção de palmito com o objetivo de baixar o pH a limites inferiores a 4,5, desfavorecendo o desenvolvimento do Clostridium botulinum, assegurando portanto a saúde do consumidor. Normalmente o ácido cítrico é utilizado para atender as exigências, no entanto admite-se que outros ácidos podem ser utilizados com vantagem quanto aos aspectos sensoriais. Este trabalho teve por objetivo avaliar o efeito da acidificação do palmito de pupunha, cultivado em Pernambuco, com diferentes ácidos sobre as características do produto em conserva. Foram testados os ácidos: cítrico, málico, láctico e tartárico na estabilidade do produto embalado em frascos de vidro com capacidade para 600 ml e quanto às características organolépticas. Os resultados obtidos demonstraram a viabilidade da acidificação do palmito de pupunha com esses diferentes ácidos sem prejuíso nas características físicas, químicas, organolépticas e microbiológicas
Vogelsgesang, Martin. "Rho-ADP-ribosylierende Exoenzyme von Clostridium botulinum und Clostridium limosum : Untersuchungen zur Substratspezifität und Interaktion mit Ral-GTPasen /." [S.l. : s.n.], 2005. http://www.gbv.de/dms/bs/toc/513916806.pdf.
Full textSharkey, Freddie. "Toxin gene expression in Clostridium botulinum type E under different growth conditions." Thesis, University of Ulster, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274025.
Full textMcGrath, Susan. "The development of an RNA assay for Clostridium botulinum toxin gene expression." Thesis, University of Ulster, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326309.
Full textGil, Luciana Aquini Fernandes. "Produção e caracterização de quimeras recombinantes C e D de Clostridium botulinum." Universidade Federal de Pelotas, 2012. http://repositorio.ufpel.edu.br/handle/ri/2486.
Full textBovine Botulism is a lethal intoxication caused by the ingestion of the neurotoxins produced by Clostridium botulinum types C and D that inhibit the release of acetylcholine at the neuromuscular junction leading to death by flaccid paralysis. It produces important economic losses, being a major cause of casualties in cattle in several regions of Brazil. The control of the disease depends on the presence of neutralizing antibodies against botulinum neurotoxins (BONTs) in immunized cattle. Immunization is obtained inoculating toxoids produced from cultures of selected strains of C. botulinum types C and D, whose industrial production has limitations concerning efficiency and productivity. An alternative to the use of these toxoids is the production of recombinant antigens with high levels of purity and antigenicity. The C-terminal fraction of the heavy chain of botulinum neurotoxins has been the main target in the development of recombinant vaccines with promising results. In this work, two recombinant bivalent chimeras for the control of bovine botulism consisting of the neuronal receptor binding domains (NRBDs) of botulinum C and D toxins were efficiently produced in Escherichia coli. They were characterized and evaluated in mice, with promising results. Both the recombinant chimeras rLTB-C-D and rC-D were produced by cloning and expressing a synthetic gene encoding the C-terminal portion of both BONTs. The former also included the preferred codons of the E. coli heat labile enterotoxin B subunit (LTB), a potent humoral immune adjuvant. The levels of expression of the recombinant antigens were satisfactory, yielding approximately 100 mg of each recombinant antigen per liter of culture. An ELISA performed to assess the antigenicity of the molecules showed that both were recognized by sera of immunized mice suggesting the preservation of epitopes with the properties of native BONTs. Both chimeras induced high levels of neutralizing antibodies without undesirable effects. The level of neutralizing antibodies of the groups inoculated with equimolar concentrations of rLTB-C-D and rC-D containing Aluminum Hydroxide as adjuvant were similar, confirming the adjuvant properties of LTB. These results demonstrated that the recombinant chimeras were immunogenic. Sera from mice inoculated with commercial vaccines were also analyzed by ELISA using as antigens rC and rD, corroborating the neutralization.
O botulismo bovino é uma intoxicação letal causada pela ingestão da neurotoxina produzida pelo Clostridium botulinum principalmente dos tipos C e D que atua inibindo a liberação de acetilcolina na junção neuromuscular levando à morte por paralisia flácida, com grande importância econômica e sanitária, sendo uma das principais causas de morte em bovinos adultos no Brasil. O controle imunológico do botulismo bovino depende da presença de anticorpos neutralizantes contra as neurotoxinas botulínicas (NBOTs) no momento da ingestão da toxina pré-formada, por meio de imunização dos animais. Atualmente, a imunização é realizada com toxóides obtidos da detoxificação do extrato de cultivos de cepas selecionadas de C. botulinum dos tipos C e D que apresentam limitações quanto à eficiência e produção. Uma alternativa ao uso dos toxóides clássicos é a produção de vacinas recombinantes usando antígenos específicos de alta pureza e imunogenicidade. A fração C-terminal da cadeia pesada da neurotoxina botulínica tem sido o alvo principal no desenvolvimento de alternativas recombinantes a serem utilizadas como vacinas. Neste trabalho, duas quimeras recombinantes bivalentes compostas pelos domínios de ligação ao receptor neuronal (DLRNs) foram produzidas em Escherichia coli, caracterizadas e avaliadas em camundongos. As quimeras recombinantes rLTB-C-D e rC-D foram produzidas a partir da clonagem e expressão de um gene sintético que codifica a porção C-terminal das NBOTs construído com os códons preferenciais de E. coli e a subunidade B da enterotoxina termolábil de E. coli (LTB), um potente adjuvante da resposta imune humoral. O nível de expressão dos antígenos foi de aproximadamente 100mg de cada antígeno recombinante por litro de cultura. Um ELISA realizado para avaliar a antigenicidade das moléculas mostrou que ambas foram reconhecidas pelos soros padrões, sugerindo conservação de epitopos semelhantes aos dos DLRNs nativos. Ambas as quimeras foram inócuas para os camundongos, os quais não apresentaram lesões no local da inoculação bem como alteração de comportamento. Nos soros dos camundongos inoculados com as quimeras recombinantes foi possível detectar níveis de anticorpos neutralizantes. O grupo inoculado com a rLTB-C-D apresentou nível de anticorpos neutralizantes semelhante ao do grupo rC-D + hidróxido de alumínio confirmando o potencial adjuvante da LTB. As quantidades de antígenos utilizados foram equimolares. Esses resultados demonstram que as quimeras recombinantes foram imunogênicas. Os soros dos camundongos inoculados com as diferentes vacinas também foram analisados por ELISA indireto utilizando rC e rD como antígenos. Os dados obtidos neste ELISA corroboram os dados da soroneutralização.
OUAKNINE, ROGER. "La conservation des aliments par ionisation ; les problemes poses par clostridium botulinum." Strasbourg 1, 1987. http://www.theses.fr/1987STR10748.
Full textRheinhart, Courtney Elizabeth. "Clostridium botulinum toxin development in refrigerated reduced oxygen packaged Atlantic croaker (Micropogonias undulatus)." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/32440.
Full textMaster of Science in Life Sciences
Cleverley, Stephen. "Exploring the role of the small GTPase Rho in T-lymphocyte biology." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.311966.
Full textArritt, Fletcher M. III. "The Effects of Modified Atmosphere Packaging on Toxin Production by Clostridium botulinum in Raw Aquacultured Flounder Fillets and Fully Cooked Breaded and Battered Pollock Portions." Diss., Virginia Tech, 2004. http://hdl.handle.net/10919/28790.
Full textPh. D.
Fach, Patrick. "Évaluation des techniques de biologie moléculaire pour la détection et le typage des Clostridium pathogènes en agro-alimentaire : application à Clostridium perfringens et Clostridium botulinum." Compiègne, 1994. http://www.theses.fr/1994COMPD689.
Full textRagazani, Adriana Valim Ferreira [UNESP]. "Ocorrência de clostrídios patogênicos em solo de pastagem da micro-região de Jaboticabal, SP." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/103894.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Entre as espécies de Clostridium de importância em patologia animal, destaca-se o Clostridium perfringens, o Clostridium botulinum, o Clostridium chauvoei. O objetivo desta pesquisa foi verificar a presença de bactérias anaeróbias esporuladas (Clostridium sp), assim como, identificar as espécies de Clostridium patogênicos para a saúde animal, principalmente de bovinos, no solo de pastagem da micro-região de Jaboticabal, SP. Foram coletadas 250 amostras de solo e realizadas contagens de bactérias esporuladas do gênero Clostridium e identificação das espécies patogênicas presentes. Os resultados permitiram demonstrar a contagem de UFC de Closrtidium sp com média em log 10 igual a 2,79, sendo que os valores mínimo e máximo obtido foram 2,15 e 3,68 respectivamente. Para caracterização e identificação, os resultados permitiram identificar a presença de bactérias anaeróbias esporuladas em 233 amostras (93,2%), entre estas 180 eram do gênero Clostridium...
The species of Clostridium of major importance to animal pathology are Clostridium perfringens, Clostridium botulinum, and C. chauvoei. Considering this, the objective of this research was to verify the presence of anareobic sporulate bacteria (Clostridium sp), and also identify the species of pathogenic Clostridium for the animal health, mostly to bovine, in pasture soil of Jaboticabal-SP. A total of 250 samples were collected and used to determine the number of sporulated bacteria from Clostridium genderand identify the pathogenic species present. The results demonstrated that the average number of CFU in log 10 of Closrtidium sp was 2,79, and the minimum and maximum values obtained were 2,15 and 3,68 respectively. After characterization and isolation and identification, the results showed the presence of 233 samples (93,2%) of sporulated bacteria, of these 180 were of Clostridium gender. The biochemical tests were identified in 42 samples, being 23 samples (9,2%) of Clostridium perfringens, 13 samples (5,2%) of Clostridium botulinum and 6 samples (2,4%) of C. chauvoei ...(Complete abstract, click electronic access below)
Ferreira, Rosa Maria Moraes. "Contaminação ambiental pelo Clostridium Botulinum tipos C e D de valas de captação hidrica e cultivo do microrganismo em um sistema experimental." [s.n.], 2002. http://repositorio.unicamp.br/jspui/handle/REPOSIP/316869.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia
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Mestrado
Hunter, Leonie C. "The role of Clostridium botulinum type C in the pathogenesis of equine grass sickness." Thesis, University of Edinburgh, 2001. http://hdl.handle.net/1842/24726.
Full textLux, Romy [Verfasser]. "Glutamat-Aufnahme und -Freisetzung durch Astrozyten nach Clostridium botulinum C3-Protein-Behandlung / Romy Lux." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2009. http://d-nb.info/1023783649/34.
Full textPiepgras, Johannes [Verfasser]. "Der Einfluss von Clostridium botulinum C3 Proteinen auf die neuronale Glutamat-Aufnahme / Johannes Piepgras." Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2019. http://d-nb.info/1201650356/34.
Full textCampbell, Kathryn Deirdre. "Molecular inter-relationships of psychrotrophic Clostridium botulinum based on 23S rRNA and BoNT genes." Thesis, University of Reading, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.262610.
Full textDukart, Irene [Verfasser]. "Charakterisierung der Interaktion des C2-Toxins von Clostridium botulinum mit Cyclophilin A / Irene Dukart." Ulm : Universität Ulm. Medizinische Fakultät, 2014. http://d-nb.info/1049238400/34.
Full textZeiller, Matthias [Verfasser], and Anton [Akademischer Betreuer] Hartmann. "Systemische Kolonisierung von Weißklee (Trifolium repens) durch Clostridium botulinum / Matthias Zeiller. Betreuer: Anton Hartmann." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2016. http://d-nb.info/1110749392/34.
Full textIsmaiel, Adnan A. "The inhibition of Clostridium botulinum growth and toxin production by essential oils of spices." Diss., Virginia Polytechnic Institute and State University, 1987. http://hdl.handle.net/10919/77803.
Full textPh. D.
Ragazani, Adriana Valim Ferreira. "Ocorrência de clostrídios patogênicos em solo de pastagem da micro-região de Jaboticabal, SP /." Jaboticabal : [s.n.], 2007. http://hdl.handle.net/11449/103894.
Full textBanca: Maria Luiza Poiatti
Banca: Alessandra Aparecida Medeiros
Banca: Hélio José Montassier
Banca: Antônio Carlos Monteiro
Resumo: Entre as espécies de Clostridium de importância em patologia animal, destaca-se o Clostridium perfringens, o Clostridium botulinum, o Clostridium chauvoei. O objetivo desta pesquisa foi verificar a presença de bactérias anaeróbias esporuladas (Clostridium sp), assim como, identificar as espécies de Clostridium patogênicos para a saúde animal, principalmente de bovinos, no solo de pastagem da micro-região de Jaboticabal, SP. Foram coletadas 250 amostras de solo e realizadas contagens de bactérias esporuladas do gênero Clostridium e identificação das espécies patogênicas presentes. Os resultados permitiram demonstrar a contagem de UFC de Closrtidium sp com média em log 10 igual a 2,79, sendo que os valores mínimo e máximo obtido foram 2,15 e 3,68 respectivamente. Para caracterização e identificação, os resultados permitiram identificar a presença de bactérias anaeróbias esporuladas em 233 amostras (93,2%), entre estas 180 eram do gênero Clostridium...(Resumo completo, clicar acesso eletrônico abaixo)
Abstract: The species of Clostridium of major importance to animal pathology are Clostridium perfringens, Clostridium botulinum, and C. chauvoei. Considering this, the objective of this research was to verify the presence of anareobic sporulate bacteria (Clostridium sp), and also identify the species of pathogenic Clostridium for the animal health, mostly to bovine, in pasture soil of Jaboticabal-SP. A total of 250 samples were collected and used to determine the number of sporulated bacteria from Clostridium genderand identify the pathogenic species present. The results demonstrated that the average number of CFU in log 10 of Closrtidium sp was 2,79, and the minimum and maximum values obtained were 2,15 and 3,68 respectively. After characterization and isolation and identification, the results showed the presence of 233 samples (93,2%) of sporulated bacteria, of these 180 were of Clostridium gender. The biochemical tests were identified in 42 samples, being 23 samples (9,2%) of Clostridium perfringens, 13 samples (5,2%) of Clostridium botulinum and 6 samples (2,4%) of C. chauvoei ...(Complete abstract, click electronic access below)
Doutor
Carpusca, Irina. "The ADP-ribosylating mosquitocidal toxin (MTX) from bacillus Sphaericus : Role of its ricin-like domain in autoinhibition and translocation." Université Louis Pasteur (Strasbourg) (1971-2008), 2005. http://www.theses.fr/2005STR13003.
Full textJunqueira, Valeria Christina Amstalden. "Avaliação da incidencia de Clostridium botulinum e da produção de toxina em mortadela e presunto." [s.n.], 1994. http://repositorio.unicamp.br/jspui/handle/REPOSIP/254645.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos
Made available in DSpace on 2018-07-19T17:23:54Z (GMT). No. of bitstreams: 1 Junqueira_ValeriaChristinaAmstalden_M.pdf: 2459645 bytes, checksum: d1db248b13652657a6e8d7dedd8ccfcf (MD5) Previous issue date: 1994
Resumo: Na presente pesquisa foi realizada uma avaliação do risco de transmissão de botulismo por mortadela e presunto. Numa primeira fase, foram examinadas amostras de mortadela e' presunto cozido, coletadas ao acaso no varejo do município de Campinas - SP. A partir de 25 amostras de cada produto, em quadruplicata de 75g, num total de 100 subamostras, foi calculado o Número Mais Provável (NMP) de C. botulinum por kg de mortadela e presunto. Nesta fase, ainda foram determinados o tempo e a temperatura de estocagem na revenda, bem como o pH, atividade de água, concentração de cloreto de sódio e de nitrito de sódio e umidade desses produtos. Na segunda fase, mortadela e presunto elaborados por nós e artificialmente contaminados com 104 esporos de C. botulinum tipos A e 8 por amostra foram avaliados quanto à possibilidade de toxigênese do microrganismo, quando submetidos à estocagem inadequada, à temperatura de 30°C. Os resultados obtidos permitiram estimar a incidência de Clostridium botulinum na ordem de O,27NMP/kg de mortadela e inferior a O, 13NMP/kg de presunto. A formação de toxina botulínica foi observada nas amostras de presunto artificialmente contaminadas, após 12 dias de estocagem à temperatura de 30°C. Ao fim de 28 dias de estocagem, à mesma temperatura, não foi detectada toxina nas amostras de mortadela
Abstract: The risk of transmitting botulism via mortadella and ham was evaluated in this research. In the first phase, samples of mortadella and cooked ham were acquired at random from shops in the region of Campinas - SP. Twenty five samples of each product were acquired and examined in quadruplicate (75g each) giving a total of 100 sub-samples. The Most Probable Number (MPN) for C. botulinum per kg of mortadella or ham, was calculated. The storage times and temperatures at the distributers were also determined plus the pH, water activity, sodium chloride and sodium nitrite concentrations and water content. In the second phase, mortadella and ham were produced in the pil0t plant and contamined with 104 C. botulinum types A and B spores per sample. These samples were evaluated to determine the possibility of toxin formation by the microorganism when the products were submitted to inadequate storage at a temperature of 30°C. The results obtained allowed for the estimation of an incidence of C. botulinum at a level of 0,27 MPN/kg in mortadella and below 0,13 MPN/kg in ham. The formation of botulinum toxin was observed after 12 days of storage at 30°C in the artificially contamined ham samples. After 28 days of storage at the same temperature, no toxin was detected in the samples of mortadella
Mestrado
Mestre em Tecnologia de Alimentos
Reddy, Divya Shree A. "Iron Requirement of Clostridiiyum Botulinum Type A and Characterization of Iron-Sulfur Proteins in Nitrite Treated and Untreated Botulinal Cells." DigitalCommons@USU, 1985. https://digitalcommons.usu.edu/etd/5303.
Full textPhillips, Daphne 1956. "Safety studies with proteolytic Clostridium botulinum in high-moisture bakery products packaged under modified atmospheres." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=84412.
Full textSubsequent studies were directed at determining the levels of additional barriers that could be used to ensure the safety of high-moisture MAP crumpets. While ethanol vapour proved to be an effective additional barrier in crumpets (100-g, [aw 0.990, pH 6.5]) challenged with ~102 spores/g of C. botulinum, spoilage preceded toxigenesis due to absorption of ethanol from the package headspace by crumpets. Modelling studies in Trypticase Peptone Glucose Yeast (TPGY) broth confirmed the anti-botulinal nature of ethanol and showed that a level of ~4% (vol/vol) could be used for complete inhibition of this pathogen, depending on the aw and pH of the growth medium. However, while ethanol vapour could be used to inhibit the growth of C. botulinum in high-moisture crumpets, its anti-botulinal efficacy was influenced by the method of crumpet leavening (yeast v chemical).
Preliminary studies were also done to assess the potential of mastic oil, a novel inhibitor, against C. botulinum. While direct and indirect application of ethanolic extracts of mastic oil inhibited the growth of C. botulinum in vivo, they failed to do so in crumpets.
Yusof, Farida Zuraina Md. "The distribution of toxin genes among isolates of Clostridium botulinum Group II from different environments." Thesis, University of Ulster, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274549.
Full textPospiech, Janina Marta Lucia. "Untersuchung von Gärresten und Gärsubstraten aus landwirtschaftlichen Biogasanlagen des Freistaates Sachsen: Auswahl und Etablierung von bakteriologischen und molekularbiologischen Verfahren zum Nachweis ausgewählter Indikatorkeime." Doctoral thesis, Universitätsbibliothek Leipzig, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:15-qucosa-189760.
Full textHaug, Gerd. "Clostridium-botulinum-C2-Toxin Interaktion der Toxinkomponenten und Translokation der Enzymkomponente in das Zytosol von Zielzellen /." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=974136484.
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