Dissertations / Theses on the topic 'Cloning tyrosine kinase genes'
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Jones, P. F. "Cloning and expression of tyrosine kinase genes." Thesis, University of Oxford, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382626.
Full textSuen, Ki-Ling. "Cloning of protein kinase genes from a carrot cDNA library." Diss., Georgia Institute of Technology, 1991. http://hdl.handle.net/1853/25431.
Full textSchweighoffer, Edina. "The role of Syk protein tyrosine kinase in B cell development and function." Thesis, Open University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.250493.
Full textCoffer, Paul James. "The identification, cloning and characterisation of novel mammalian protein-serine kinase genes." Thesis, Open University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293298.
Full textMoncrieff, Colin Lindsay. "Cloning and characterisation of a novel DMPK-related gene : CDC42BPB." Thesis, University of Glasgow, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.323439.
Full textChen, Yi-Jun Grace. "Cloning and expression of three protein kinase genes of Colletotrichum gloeosporioides f. sp. malvae." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ40402.pdf.
Full textPylayeva, Yuliya. "Role of focal adhesion kinase in mammary gland tumorigenesis /." Access full-text from WCMC, 2008. http://proquest.umi.com/pqdweb?did=1528351831&sid=1&Fmt=2&clientId=8424&RQT=309&VName=PQD.
Full textPrata, Rodrigo Ferreira. "Papel do Tyrosine receptor Kinase B (TrkB) na regulação de genes do metabolismo hepático de colesterol e triglicerídeos." reponame:Repositório Institucional da UFABC, 2012.
Find full textSummy, Justin Matthew. "Functional domain contributions to signaling specificity between the non-receptor tyrosine kinases c-src and c-yes." Morgantown, W. Va. : [West Virginia University Libraries], 2001. http://etd.wvu.edu/templates/showETD.cfm?recnum=2239.
Full textTitle from document title page. Document formatted into pages; contains vi, 195 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 182-190).
AGNES, FRANCOIS. "Etude de la structure des genes codant les recepteurs tyrosine kinase avec des domaines de type immunoglobuline : un modele d'evolution moleculaire." Paris 6, 1995. http://www.theses.fr/1995PA066005.
Full text朱江 and Jiang Zhu. "RET transcriptional regulation by HOXB5 in Hirschsprung's disease." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2012. http://hdl.handle.net/10722/193397.
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Barker, Andrew Bellamy. "Expression, Cloning, and Sequencing of Putative Insulin Signaling Genes Involved in Diapause in the Flesh Fly Sarcophaga crassipalpis." Digital Commons @ East Tennessee State University, 2005. https://dc.etsu.edu/etd/1041.
Full textMa, Grace. "Heterodimeric deoxyguanosine/deoxyadenosine kinase from Lactobacillus acidophilus R-26 : affinity protein purification, molecular cloning, sequence of the genes, and expression in E. coli /." The Ohio State University, 1993. http://rave.ohiolink.edu/etdc/view?acc_num=osu148784197535822.
Full textSalsman, Scott J. "Redox regulation of protein tyrosine phosphatases in cell membrane receptor-mediated signal transduction." Oklahoma City : [s.n.], 2005.
Find full textJunior, Euclides Matheucci. "Isolamento e análise funcional do gene que codifica uma proteína serina-treonina quinase que modula a expressão de genes regulados por carboidratos em Trichoderma reesei." Universidade de São Paulo, 2000. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-18092015-163705/.
Full textA gene homologue to the members of the AMP-activated/SNFl protein kinase subfamily, TrSNF1, was isolated from the filamentous fungus Trichoderma reesei. The predicted protein of 692 amino acids has a kinase domain, that share 42 % identity and 59 % similarity to that of serine/threonine protein kinase of this family. In Saccharomyces cerevisiae, the SNFl protein kinase is required for expression of glucose repressed genes in response to withdrawal of glucose from the medium. Expression of the Trichoderma reesei SNF1-related sequence in yeast SNFl mutant restores SNFl function. The TrSNFl antisense expression in T. reesei causes a control alteration in the glucose-regulated gene CBHI. The observed structural identity with the AMP-activated/SNFl protein kinase subfamily, and the functional similarity to the yeast SNFl suggest that the TrSNFl may be involved in the regulation of sugar metabolism in Trichoderma reesei.
Chen, Min. "Studies of the SH2- and SH3- containing adaptor, Nck /." 1999. http://wwwlib.umi.com/dissertations/fullcit/9934031.
Full textchen, Hui-Lin, and 陳慧玲. "Cloning and characterization of a FGFR tyrosine kinase from Aedes aegypti." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/27772796482258781416.
Full textLiao, chen yee, and 廖千億. "Cloning,Expression , and Structure studies of SH2 Domain of Bruton's Tyrosine Kinase." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/09038241850831693475.
Full text陳俊宏. "Molecular cloning and characterization of kinase genes from lily (Lilium longiflorum)." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/44629284594846786695.
Full text國立中興大學
農業生物科技學研究所
88
Signal transduction pathway involving protein kinase is required for flower development in plants. To study the signal transduction pathway regulated by protein kinase in lily, the cloning and characterization of protein kinase genes from lily is necessary. Using RT-PCR and 5’-RACE strategies, a cDNA showed high homology to ATPK1/ATPK6, a serine/threonine protein kinase gene with unknown function from Arabidopsis, was isolated from lily floral buds and named LPK1 (lily protein kinase gene 1). LPK1, which showed 64% identity and 74% similarity to ATPK1/ATPK6 with the highest homology (about 87% identity) in the central catalytic domain (about 243 amino acids), encodes a hydrophilic polypeptide of 479 amino acids with the centrally located catalytic domain containing all 11 conserved subdomains of eukaryotic protein kinase, and have the greatest homology to p70 ribosomal S6 kinases, protein kinase C and protein kinase A family of protein-serine kinases. The catalytic domain also contains motifs characteristic of protein-serine/threonine kinases such as GXGXXG and HRDLKPEN. Other motifs such as putative autophosphorylation site (XRXXSX) and a lysine-rich domain (42% lysine) were also found in the catalytic domain of LPK1. Northern blot analysis indicated that LPK1 was developmental regulated. LPK1 RNA was strongly detected in young floral buds and its expression was absent in mature floral buds. Low expression for LPK1 was also observed in leaf and stem. Ectopic expression of LPK1 in transgenic Arabidopsis plants reduced the petal and stamen growth by inhibiting the cell expansion. The 35S::LPK1 transgenic plants were sterile due to the reduction of filaments elongation of stamens. A 1911 bp cDNA encoding 510 amino acids of a lily pyruvate kinase (LPYK) was also cloned and characterized in this research during the cloning of LPK1 cDNA.
Lee, Tzuu-Fen, and 李祖芬. "Cloning and Analysis of the Ripening-related Genes Encoding Protein Kinase in Banana." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/26601213171978414721.
Full text國立臺灣大學
園藝學系研究所
85
Reversible protein phosphorylation mechanism is thought to play a key role in diverse biological signal transduction systems in animal, yeast, and plant. To identify protein kinases which functions during fruit ripening, we have isola ted several cDNA clones from Musa spp. using PCR and degenerate oligonucleotid es designed based on the conserved regions of the catalytic domain of protein kinases. One of the cDNA clones (pBPK3) was completely sequenced and character ized.The cDNA clone is 1172bp in length and encodes a polypeptide of 266 amino acids with a calculated molecular mass of 29,814 Da. The putative BPK3 protei n shows extensive homology to MAPKK genes from Arabidopsis, yeast and vertebra tes. Major (1.35kb) and minor (2.5kb) transcripts were detected in bract, anth er, and most abundantly in ripened banana fruit. The mRNA level of BPK3 increa sed markedly at stage 2 fruit, remained nearly constant during ripening stages and slightly decreased at stage 7 fruit. To investigate the structure of pro tein kinase genes, a genomic library of Musa spp. was screened using four cDNA s (pBPK3、pBPK6、pBPK9 and pBPK10) as probes. Thirty-three positive clones wer e obtained by screening 1.5x106 plaques under high-stringency hybridization co ndition. One of the genomic clone lBPSK70 was characterized and revealed the p resence of only one exon. The gene is 3644 bp long, and the encoded protein co ntains an open reading frame of 348 amino acids with calculated molecular mass of 38,329 Da and predicted pI of 9.41 . Sequence comparison revealed that pBP K3 cDNA was derived from BPSK70 gene. The two protein kinase genes fragments ( BPK6 and PK5) were also sequenced, and the predicted polypeptides show closely related to receptor-like serine/threonine kinase / or Raf and ribosomal prote in kinase, respectively. Northern analysis revealed that the two genes express ed in ripening banana fruit.The existence of these protein kinase genes has in dicated that the signaling pathway during fruit ripening may resemble eukaryot ic MAPK signaling cascades.
MOHAMED, AHMED. "Identification of Genes Involved in the C. elegans VAB-1 Eph Receptor Tyrosine Kinase Signaling Pathway." Thesis, 2011. http://hdl.handle.net/1974/6615.
Full textThesis (Ph.D, Biology) -- Queen's University, 2011-07-28 16:20:31.957
Parnell, Laurence David. "Cloning and characterization of a serine/threonine protein kinase of Dictyostelium discoideum, with a phylogenetic analysis of serine/threonine and tyrosine protein kinases." 1993. http://catalog.hathitrust.org/api/volumes/oclc/31338222.html.
Full textTypescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 398-488).
Hsu, Yi-Hsuan, and 許翼軒. "Cloning of Deoxynucleoside Monophosphate Kinase and Deoxynucleoside Diphosphate Kinase Genes from Thermus thermophilus HB8 and Establishment of a New Enzymatic Method for Biosynthesis of Deoxynucleoside Triphosphates." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/3t59rs.
Full text國立清華大學
分子醫學研究所
102
Deoxynucleoside triphosphates (dNTP) are essential biological materials for synthesizing DNA. The compounds are also used in molecular biotechnology such as the polymerase chain reaction (PCR) and other DNA polymerase-based applications. Nowadays, dNTPs are commercially produced by chemical synthesis due to their low concentrations in living organisms. However, the chemical synthesis method utilizes toxic solvents and the yield is low. To solve these problems, this study attempt to establish an in vitro enzymatic method for complete biosynthesis of dNTP. The process involves two stages of enzymatic phosphorylation procedures. The deoxynucleoside monophosphates (dNMPs) are used in the first step. The products of the first and second stages are deoxynucleoside diphosphates (dNDP) and dNTP, respectively. Five genes from the Thermus thermophilus HB8, which encode four kinds of deoxynucleoside monophosphate kinases (NMKs) and one nucleoside diphosphate kinase (NDK), were cloned and expressed in high quality in E. coli BL21 (DE3) and NovaBlue (DE3). We have performed functional and kinetic property analysis of NMKs and NDK. The results showed that the five enzymes exhibited optimum activity at the pH value of 7.0 to 8.0 and at the temperature between 75OC to 80 OC. Furthermore, all of them displayed excellent thermal stability. Approximate 80% and 50% of enzyme activities were retained after incubation at 75 OC for 3 hr and 24hr, respectively. The kinetic property analysis showed that five enzymes have higher catalytic efficiency under optimum environment as compared to the mesophilic bacteria. Later, we combined these crude enzymes preliminarily to produce dNTP. The PCR performed using the enzymes-synthesized dNTP showed the same efficiency compared to the commercial one. Finally, this system provides a clean and better method of preparation that has potential for commercialization.
Tice, David Alan. "The role of C-SRC in tumorigenesis /." 1999. http://wwwlib.umi.com/dissertations/fullcit/9930109.
Full textVanDusen, Nathan J. "Hand2 function within non-cardiomyocytes regulates cardiac morphogenesis and performance." Thesis, 2014. http://hdl.handle.net/1805/6170.
Full textThe heart is a complex organ that is composed of numerous cell types, which must integrate their programs for proper specification, differentiation, and cardiac morphogenesis. During cardiac development the basic helix-loop-helix transcription factor Hand2 is dynamically expressed within the endocardium and extra-cardiac lineages such as the epicardium, cardiac neural crest cells (cNCCs), and NCC derived components of the autonomic nervous system. To investigate Hand2 function within these populations we utilized multiple murine Hand2 Conditional Knockout (H2CKO) genetic models. These studies establish for the first time a functional requirement for Hand2 within the endocardium, as several distinct phenotypes including hypotrabeculation, tricuspid atresia, aberrant septation, and precocious coronary development are observed in endocardial H2CKOs. Molecular analyses reveal that endocardial Hand2 functions within the Notch signaling pathway to regulate expression of Nrg1, which encodes a crucial secreted growth factor. Furthermore, we demonstrate that Notch signaling regulates coronary angiogenesis via Hand2 mediated modulation of Vegf signaling. Hand2 is strongly expressed within midgestation NCC and endocardium derived cardiac cushion mesenchyme. To ascertain the function of Hand2 within these cells we employed the Periostin Cre (Postn-Cre), which marks cushion mesenchyme, a small subset of the epicardium, and components of the autonomic nervous system, to conditionally ablate Hand2. We find that Postn-Cre H2CKOs die shortly after birth despite a lack of cardiac structural defects. Gene expression analyses demonstrate that Postn-Cre ablates Hand2 from the adrenal medulla, causing downregulation of Dopamine Beta Hydroxylase (Dbh), a gene encoding a crucial catecholaminergic biosynthetic enzyme. Electrocardiograms demonstrate that 3-day postnatal Postn-Cre H2CKO pups exhibit significantly slower heart rates than control littermates. In conjunction with the aforementioned gene expression analyses, these results indicate that loss of Hand2 function within the adrenal medulla results in a catecholamine deficiency and subsequent heart failure.