Relevant bibliographies by topics / Cleanliness assay

Academic literature on the topic 'Cleanliness assay'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Cleanliness assay"

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Durkee, John. "Using simple science to assay surface cleanliness." Metal Finishing 106, no. 2 (February 2008): 49–51. http://dx.doi.org/10.1016/s0026-0576(08)80040-2.

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SEEGER, KLAUS, and MANSEL W. GRIFFITHS. "Adenosine Triphosphate Bioluminescence for Hygiene Monitoring in Health Care Institutions." Journal of Food Protection 57, no. 6 (June 1, 1994): 509–13. http://dx.doi.org/10.4315/0362-028x-57.6.509.

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An investigation was conducted to assess the practical use of an adenosine triphosphate (ATP) bioluminescence assay to evaluate the effectiveness of cleaning and sanitizing meat slicers in eight health care institutions. The ATP bioluminescence assay was compared to conventional swabbing techniques using standard plate count to enumerate microbial load. Assays were performed on meat slicers before use, after slicing a meat product and after sanitizing. There was a general overall agreement in results obtained by both methods but the ATP assay gave a better indication of the cleanliness of the meat slicer as it was able to detect the presence of meat residues left on the blade after improper sanitation. Results were available within 5 min using the ATP bioluminescence method, thus providing an opportunity for immediate remedial action.
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SHIBATA, Hirofumi, Kazuyoshi KAWAZOE, Takahiro SHIBATA, Shuji FUSHITANI, Miho WATANABE, Tomoko TAKAGAI, Tamiko NAGAO, Mami AZUMA, and Kazuo MINAKUCHI. "Investigation of the Cleanliness of Hospital Environmental Surfaces by Adenosine Triphosphate Bioluminescence Assay." Japanese Journal of Infection Prevention and Control 29, no. 6 (2014): 417–23. http://dx.doi.org/10.4058/jsei.29.417.

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Cooper, Moogega, Myron T. La Duc, Alexander Probst, Parag Vaishampayan, Christina Stam, James N. Benardini, Yvette M. Piceno, Gary L. Andersen, and Kasthuri Venkateswaran. "Comparison of Innovative Molecular Approaches and Standard Spore Assays for Assessment of Surface Cleanliness." Applied and Environmental Microbiology 77, no. 15 (June 7, 2011): 5438–44. http://dx.doi.org/10.1128/aem.00192-11.

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ABSTRACTA bacterial spore assay and a molecular DNA microarray method were compared for their ability to assess relative cleanliness in the context of bacterial abundance and diversity on spacecraft surfaces. Colony counts derived from the NASA standard spore assay were extremely low for spacecraft surfaces. However, the PhyloChip generation 3 (G3) DNA microarray resolved the genetic signatures of a highly diverse suite of microorganisms in the very same sample set. Samples completely devoid of cultivable spores were shown to harbor the DNA of more than 100 distinct microbial phylotypes. Furthermore, samples with higher numbers of cultivable spores did not necessarily give rise to a greater microbial diversity upon analysis with the DNA microarray. The findings of this study clearly demonstrated that there is not a statistically significant correlation between the cultivable spore counts obtained from a sample and the degree of bacterial diversity present. Based on these results, it can be stated that validated state-of-the-art molecular techniques, such as DNA microarrays, can be utilized in parallel with classical culture-based methods to further describe the cleanliness of spacecraft surfaces.
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Hung, I.-Chen, Hao-Yuan Chang, Aristine Cheng, An-Chi Chen, Ling Ting, Mei-Wen Chen, Yeur-Hur Lai, and Wang-Huei Sheng. "Application of a fluorescent marker with quantitative bioburden methods to assess cleanliness." Infection Control & Hospital Epidemiology 39, no. 11 (September 17, 2018): 1296–300. http://dx.doi.org/10.1017/ice.2018.222.

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AbstractBackgroundImprovement of environmental cleaning in hospitals has been shown to decrease in-hospital cross transmission of pathogens. Several objective methods, including aerobic colony counts (ACCs), the adenosine triphosphate (ATP) bioluminescence assay, and the fluorescent marker method have been developed to assess cleanliness. However, the standard interpretation of cleanliness using the fluorescent marker method remains uncertain.ObjectiveTo assess the fluorescent marker method as a tool for determining the effectiveness of hospital cleaning.DesignA prospective survey study.SettingAn academic medical center.MethodsThe same 10 high-touch surfaces were tested after each terminal cleaning using (1) the fluorescent marker method, (2) the ATP assay, and (3) the ACC method. Using the fluorescent marker method under study, surfaces were classified as totally clean, partially clean, or not clean. The ACC method was used as the standard for comparison.ResultsAccording to the fluorescent marker method, of the 830 high-touch surfaces, 321 surfaces (38.7%) were totally clean (TC group), 84 surfaces (10.1%) were partially clean (PC group), and 425 surfaces (51.2%) were not clean (NC group). The TC group had significantly lower ATP and ACC values (mean ± SD, 428.7 ± 1,180.0 relative light units [RLU] and 15.6 ± 77.3 colony forming units [CFU]/100 cm2) than the PC group (1,386.8 ± 2,434.0 RLU and 34.9 ± 87.2 CFU/100 cm2) and the NC group (1,132.9 ± 2,976.1 RLU and 46.8 ± 119.2 CFU/100 cm2).ConclusionsThe fluorescent marker method provided a simple, reliable, and real-time assessment of environmental cleaning in hospitals. Our results indicate that only a surface determined to be totally clean using the fluorescent marker method could be considered clean.
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Huang, Yu-Shan, I.-Chen Hung, Mei-Ling Chen, Jann-Tay Wang, Wang-Huei Sheng, Yee-Chun Chen, and Shan-Chwen Chang. "Evaluating the efficacy of environmental cleanliness by adenosine triphosphate bioluminescence assay at a medical center." Journal of Microbiology, Immunology and Infection 48, no. 2 (April 2015): S55. http://dx.doi.org/10.1016/j.jmii.2015.02.113.

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Hung, I.-Chen, Bao-Haw Huang, An-Chi Chen, Jann-Tay Wang, Wang-Huei Sheng, Yee-Chun Chen, and Shan-Chwen Chang. "Using feedback with adenosine triphosphate (ATP) bioluminescence assay improved the cleanliness of computerized nursing cart." Journal of Microbiology, Immunology and Infection 48, no. 2 (April 2015): S168. http://dx.doi.org/10.1016/j.jmii.2015.02.587.

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ENOKIDANI, Masafumi, Katsuya KIDA, and Akio MIYAMOTO. "Evaluation of Teat Skin Cleanliness During Milking at a Dairy Farm Using an ATP-Bioluminescence Assay." Journal of the Japan Veterinary Medical Association 66, no. 12 (2013): 847–51. http://dx.doi.org/10.12935/jvma.66.847.

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Ferreira, Adriano Menis, Denise de Andrade, Marcelo Alessandro Rigotti, and Maria Verônica Ferrareze Ferreira. "Condition of cleanliness of surfaces close to patients in an intensive care unit." Revista Latino-Americana de Enfermagem 19, no. 3 (June 2011): 557–64. http://dx.doi.org/10.1590/s0104-11692011000300015.

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Surface cleaning is a well-known control procedure against the dissemination of microorganisms in the hospital environment. This prospective study, carried out in an intensive care unit over the course of 14 days, describes the cleaning/disinfection conditions of four surfaces near patients. In total, 100 assessments of the surfaces were carried out after they were cleaned. Three methods were used to evaluate cleanliness: a visual inspection, an adenosine triphosphate (ATP) bioluminescence assay and testing for the presence of Staphylococcus aureus and meticillin-resistant Staphylococcus aureus/MRSA. Respectively, 20%, 80% and 16% of the assessments by the visual method, ATP and the presence of Staphylococcus aureus/MRSA failed. There were statistically significant differences (p<0.05) between the rates of failure of the cleaning using the ATP method, compared to the visual and microbiological methods. The visual inspection was not a reliable measure to evaluate surface cleanliness. The results demonstrated that the adopted cleaning routine should be reconsidered.
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Masia, Maria Dolores, Marco Dettori, Grazia Maria Deriu, Sabina Bellu, Lisa Arcadu, Antonio Azara, Andrea Piana, Alessandra Palmieri, Antonella Arghittu, and Paolo Castiglia. "ATP Bioluminescence for Assessing the Efficacy of the Manual Cleaning Procedure during the Reprocessing of Reusable Surgical Instruments." Healthcare 9, no. 3 (March 19, 2021): 352. http://dx.doi.org/10.3390/healthcare9030352.

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Achieving sterilization by adopting proper practices is essential to ensure that surgical instruments do not transmit microorganisms to patients. As the effectiveness of sterilization mandates effective cleaning, it is necessary to verify the success of cleaning procedures. In this study, we used the adenosine triphosphate (ATP) bioluminescence method for assessing the efficacy of the manual cleaning procedure during the reprocessing of reusable surgical instruments. The ATP bioluminescence assay was performed on 140 surgical instruments of 12 different types, both before being cleaned (baseline) and after each of the cleaning procedures (i.e., decontamination, manual washing, drying, and visual inspection). For each instrument, two swabs were used as follows: one to sample the entire surface (test point 1) and the other to sample the most difficult part of the surface to clean (test point 2). Overall, for each type of instrument, there was a decrease in contamination ranging from 99.6 to >99.9% (log reduction from 2.40 to 3.76). Thus, in order to standardize the assessment of cleanliness, it may be useful to introduce the bioluminescence method into the daily routine or, at least, at regular time intervals as a complementary check combined with visual inspection. This would allow real-time verification of the achievement of an adequate level of cleanliness.
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Conference papers on the topic "Cleanliness assay"

1

FRUNDZHYAN, VG, NN UGAROVA, NI GABRIYELYAN, LI AREFÈVA, and TB PREOBRAZHENSKAY. "BIOLUMINESCENT ASSAY OF STERILITY OR CLEANLINESS IN A HOSPITAL ENVIRONMENT." In Proceedings of the 13th International Symposium. WORLD SCIENTIFIC, 2005. http://dx.doi.org/10.1142/9789812702203_0092.

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