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Dubois, Damien. "L'ilot génomique pks chez Escherichia coli : structure-fonction de la protéine ClbP et études épidémiologiques." Phd thesis, Université d'Auvergne - Clermont-Ferrand I, 2011. http://tel.archives-ouvertes.fr/tel-00612631.
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L'ilot génomique pks de Escherichia coli et d'autres Enterobacteriaceae code des synthases depolycétides et de peptides non ribosomaux qui permettent l'assemblage d'un composé hybride polycétidepeptidenon ribosomal putative. Ce composé nommé colibactine induit des cassures double-brin de l'ADN descellules eucaryotes.La machinerie enzymatique codée par l'ilot pks comporte une protéine essentielle ClbP, atypique dansce type d'ilot. Nous avons montré que ClbP possède une partie N-terminale catalytique et périplasmique, et unepartie C-terminale associée à la membrane cytoplasmique. La structure cristalline de ClbP et des expériences demutagenèse ont révélé un site actif à sérine et des caractéristiques structurales originales, qui sont compatiblesavec une activité peptidase, confirmée par des analyses biochimiques. Dix homologues de ClbP ont été identifiésin silico dans des ilots génomiques de synthases de peptides non ribosomaux d'espèces bactériennes proches etéloignées. Tous les homologues testés ont présenté une promiscuité fonctionnelle avec ClbP. ClbP est donc leprototype d'une nouvelle sous-famille de peptidases, qui sont probablement impliquées dans la maturation decomposés peptidiques non ribosomaux.Par ailleurs, nous avons réalisé deux études épidémiologiques sur la prévalence de l'ilot pks dansl'espèce E. coli dans deux contextes physiopathologiques, l'urosepsis et les cancers coliques et rectaux. L'ilotpks était significativement associé aux souches issues d'urosepsis comparé à des souches commensales, et auxsouches issues de biopsies de tumeurs coliques comparé à des souches commensales ou issues de biopsies detumeurs rectales, de diverticuloses et de lésions iléales de maladie de Crohn.
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Mukandoli, Kumuntu. "Predisposing factors of chronic low back pain (CLBP) among sedentary office workers (SOW) in Nairobi, Kenya." Thesis, University of the Western Cape, 2004. http://etd.uwc.ac.za/index.php?module=etd&.
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Chronic low back pain is a highly prevalent condition in industrialized nations. It is associated with activity limitations, disability, has significant economic impact on society and incurs personal cost. Today's working environment increasingly demands more time spent sitting due to computerization and other advances in technology. Sitting for hours without taking breaks may influence posture, and alignment of the lumbar spine. Therefore, it may influence low back pain. Kenya as a developing country has an increasing number of people involved in sedentary work. The aim of this study was to identify the predisposing factors of chronic low back pain among sedentary office workers in Nairobi. The main objectives were to establish the prevalence of chronc low back painto determine the possible predisposing factors of chronic low back pain and to determine the impact of chronic low back pain on work related quality of life among sedentary office workers in Nairobi, Kenya.
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Snelgrove, Sherrill. "A longitudinal investigation into patients' experiences of chronic low back pain (CLBP) using interpretative phenomenological analysis (IPA)." Thesis, Swansea University, 2010. https://cronfa.swan.ac.uk/Record/cronfa42594.
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Background/aim: Chronic low back pain (CLBP) is a variant of chronic pain and an overarching term for a diverse number of painful and benign conditions of the lower spine. Research has shown that CLBP challenges biomedical explanation and treatments and incurs passive coping strategies. Despite the enduring nature of CLBP there are few longitudinal studies. The aim of this investigation was to gain understandings of any consistencies and changes in the experiences of participants' experiences of living with CLBP. Design: A qualitative, longitudinal IP A research project that explored participants' pain experiences over two years (2005-2007). Methods: Semi-structured interviews were conducted with a purposeful sample of ten participants recruited from the waiting list of a chronic pain clinic. Each participant was interviewed prior to attendance and twice after treatment. The data were recorded and transcribed accounts were analysed using IPA. Results: The participants foreground the physicality of the pain. Further interpretive work showed that whilst participants emphasised the physicality of their condition they experienced embodied, multidimensional experiences characterised by loss. Most participants' continued to manage their pain within a biomedical model of understanding and behavioural focused coping strategies. In comparison, participants who experienced a period of painlessness due to medical interventions demonstrated a reappraisal of their situation and a trend towards adopting a wider, biopsychosocial understanding accompanied by changing coping strategies. Conclusion: The accounts revealed the relationship between the participants' painful body and self concept. For some participants, a respite from pain paralleled increasing psychosocial coping strategies and a future orientation that reflected changes in illness beliefs in the absence of a formal psychological intervention. In comparison, remaining participants continued to demonstrate a narrow repertoire of coping and loss orientation. Participants' responses to CLBP resonated with the grieving processes of bereaved individuals. Clinical implications are discussed with recommendations for future research.
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Gottwald, Cornelia Renate Verfasser], Harald [Akademischer Betreuer] [Gutachter] [Walach, and Stefan [Gutachter] Schmidt. "Chronischer Schmerz des unteren Rückens (CLBP)-ein medizinisches Leitsymptom unserer Kultur und eine zeitgemäße Behandlungsmöglichkeit / Cornelia Renate Gottwald ; Gutachter: Harald Walach, Stefan Schmidt ; Betreuer: Harald Walach." Frankfurt (Oder) : Europa-Universität Viadrina Frankfurt, 2017. http://d-nb.info/1142769844/34.
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Campos, Lara. "Predição da Resposta de Sucesso a um Programa de Exercício em Meio Aquático para Utentes com Dor Lombar Crónica." Master's thesis, Instituto Politécnico de Setúbal. Escola Superior de Saúde, 2014. http://hdl.handle.net/10400.26/7683.
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Relatório do Projeto de Investigação apresentado para cumprimento dos
requisitos necessários à obtenção do grau de Mestre em Fisioterapia, área de
especialização em Fisioterapia em Condições Músculo- EsqueléticasPALAVRAS-CHAVE: Dor Lombar Crónica (DLC), Factores de Prognóstico, Intensidade da Dor, Incapacidade Funcional Introdução e Objectivo: A evidência existente acerca de potenciais factores que possam predizer resultados de sucesso em utentes com DLC é não só escassa, mas sobretudo pouco consistente. O presente estudo teve como objectivo identificar factores de prognóstico para os bons resultados da Fisioterapia, a curto e médio prazo, ao nível da intensidade da dor, capacidade funcional e percepção de melhoria em indivíduos com DLC, que realizaram um programa de exercício em meio aquático. Metodologia: Foi realizado um estudo de coorte prospectivo, com 42 participantes com DLC; os quais foram submetidos a um programa de exercício aquático, com duração de 6 semanas. Os resultados do programa foram avaliados imediatamente após o seu término, e três meses após o final do tratamento. Os outcomes de interesse foram a intensidade da dor, medida pela Escala Visual Analógica (EVA), a incapacidade funcional, medida pela Quebek Back Pain Disability Scale – Versão Portuguesa (QBPDS-PT), e a percepção global de melhoria, medida pela Patient Global Impression Change Scale – Versão Portuguesa (PGIC-PT). As características sociodemográficas e clínicas avaliadas no início do estudo foram incluídas como potenciais factores de prognóstico. Como critérios de sucesso, foram utilizadas as Diferenças Clínicas Minimamente Importantes (DCMIs) definidas na literatura para os três instrumentos utilizados. Resultados: Os resultados obtidos sugerem que: 1) ao nível da intensidade da dor, as variáveis de prognóstico intensidade da dor reportada na baseline (OR= 1,049; 95% IC 1,004-1,097) e presença de irradiação para o membro inferior (OR=13,418; 95% IC 1,963- 91,716) estão significativamente associadas com os resultados de sucesso imediatamente após o programa de exercício aquático (6 semanas); e a intensidade da dor reportada na baseline está significativamente associada com os resultados de sucesso, três meses após o final do tratamento (OR=1,045; 95% IC 1,004-1,089); 2) ao nível da incapacidade funcional, apenas a pontuação na QBPDS-PT reportada na baseline se encontra estatisticamente associada com a incapacidade funcional registada 6 semanas após o início do estudo (OR=1,061 95% IC 1,009-1,115). Conclusões: Utentes com níveis mais elevados de intensidade de dor e presença de irradiação da dor para o membro inferior, no início do estudo, apresentam maior probabilidade de sucesso, ao nível da intensidade da dor, imediatamente após um programa de exercício aquático; e utentes com maiores níveis de intensidade da dor, no início do estudo, apresentam maior probabilidade de sucesso, também ao nível da intensidade da dor, três meses após o final do tratamento. Utentes com maiores níveis de incapacidade funcional no início do estudo, apresentam maior probabilidade de atingirem resultados de sucesso, ao nível da incapacidade funcional, imediatamente após o final do programa de exercício aquático.
Abstract:Introduction and Objectives: There is little evidence about potential prognostic factors that can influence the successful outcomes of patients with CLBP. The aim of this study was to assess prognostic factors for the success of an aquatic exercise program, for pain intensity, disability and global impression change, in patients with CLBP; immediately after the treatment and in a 3 months follow-up. Methodology: It was used a prospective cohort study with 42 participants, who undertake an exercise aquatic program for 6 weeks. The results of the program were assessed immediately after the treatment, and at 3 months follow-up. The primary outcomes were pain intensity, measured by Visual Analogic Scale (VAS), functional disability, measured by Quebek Back Pain Disability Scale – Portuguese Version (QBPDS-PT), and the global impression of change, measured by the Patient Global Impression Change Scale – Portuguese Version (PGIC-PT). The socio-demographic and clinical data were used as potential prognostic factors. Success with the treatment was defined considering the Minimal Clinically Important Difference (MCID) reported on the literature for the three instruments used. Results: For pain intensity, the variables pain intensity in the baseline (OR= 1,049; 95% IC 1,004-1,097) and presence of irradiating pain (OR=13,418; 95% IC 1,963-91,716), were associated with successful results for pain intensity, immediately after the end of the exercise aquatic program; and the pain intensity in the baseline were associated with successful results in the 3 months follow-up (OR=1,045; 95% IC 1,004-1,089). In what concerns to functional disability, only the score obtained in the QBPDS-PT, at the baseline, was associated with the functional disability assessed immediately after the end of the treatment (OR=1,061 95% IC 1,009-1,115). Conclusions: Participants with more pain intensity and presence of irradiating pain in the baseline, were more associated with results of success in pain intensity, immediately after a program of aquatic exercise; and participants with more pain intensity, in the baseline, were more probably associated with results of success in pain intensity, at the follow-up of 3 months. Participants with more disability at the baseline were more likely to present successful results, in functional disability, immediately after the end of the treatment.
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Infante, Meza María Soledad. "Clip." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/101664.
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Montero, Prieto María del Rosario. "Clip." Tesis, Universidad de Chile, 2007. http://repositorio.uchile.cl/handle/2250/101408.
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El siguiente texto trata sobre los procesos de creación; en especial, la
manera en que una secuencia de reglas convertidas en eventos preestablecidos,
configuran la obra en su estado final. Dichas normas se
constituyen como los puntos comunes que permiten relacionar el proceso de
producción de la obra de arte con un ámbito más abstracto como es el juego.
Por otra parte la presente tesis trata el tema del retrato fotográfico de
proyección, con esto me refiero al retrato de un sujeto en ausencia de éste. La
instantánea de aquellas cosas que lo rodean y en las cuales él imprime su huella
a través del uso y la relación que establece entre sujeto/objeto.
Por medio del análisis de distintas obras personales, se va develando la
problemática sobre la génesis del trabajo. Así mismo se analizan obras de otros
artistas, como Andreas Gursky, Sophie Calle y Miyako Ishiuchi, con las cuales
se establece por una parte la cercanía formal con mi trabajo y por otra aquellos
elementos que los alejan como referentes.
Con un breve recorrido histórico de los paradigmas básicos de la
fotografía (basándome en Barthes, Krauss y Fontcuberta) éste texto busca
aclarar dónde me sitúo y cómo se suceden mis procesos de creación en relación
al de otros artistas o personajes que transitan por ámbitos próximos a éstos,
siendo importante en todos los casos la relación entre el proyecto y las reglas
establecidas
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Kholodko, S. G. "Paper clip." Thesis, Сумський державний університет, 2014. http://essuir.sumdu.edu.ua/handle/123456789/34864.
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Sometimes, coming to work every day and using familiar objects, we do not even think that in the cup with stationery items is a tiny and unique thing glorious with its long, interesting and controversial history.
A paper clip is an instrument used to hold sheets of paper together, usually made of steel wire bent to a looped shape. Recent innovations include multi-colored plastic-coated paper clips and spring-fastened binder clips.
When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/34864
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Bocquet, Frédéric. "Remplissage des colonnes CLHP." Paris 5, 1996. http://www.theses.fr/1996PA05P010.
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Finke, Melanie [Verfasser], and Markus [Akademischer Betreuer] Bischoff. "Einfluss der HSP100/Clp ATPasen ClpB und ClpC aus Staphylococcus aureus auf die Internalisierung und das Langzeitüberleben in eukaryoten Zellen / Melanie Finke. Betreuer: Markus Bischoff." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2015. http://d-nb.info/1064868606/34.
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Golumbeanu, Monica. "Statistical Analysis of PAR-CLIP data." Thesis, KTH, Beräkningsbiologi, CB, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-124347.
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From creation to its degradation, the RNA molecule is the action field of many binding proteins with different roles in regulation and RNA metabolism. Since these proteins are involved in a large number of processes, a variety of diseases are related to abnormalities occurring within the binding mechanisms. One of the experimental methods for detecting the binding sites of these proteins is PAR-CLIP built on the next generation sequencing technology. Due to its size and intrinsic noise, PAR-CLIP data analysis requires appropriate pre-processing and thorough statistical analysis. The present work has two main goals. First, to develop a modular pipeline for preprocessing PAR-CLIP data and extracting necessary signals for further analysis. Second, to devise a novel statistical model in order to carry out inference about presence of protein binding sites based on the signals extracted in the pre-processing step.
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Wright, David. "Disentangling an unfoldase : structural studies of ClpB." Thesis, Birkbeck (University of London), 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.504736.
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ClpB is a bacterial Hsp 100 chaperone that has been shown to cooperate with the DnaKfJ (Hsp70/40) chaperone system in the removal of aggregated protein from cells. The HsplOO chaperones are members of the AAA+ super-family with two AAA+ domains per monomer. These proteins in their biologically active state from ring like oligomers, which have proved very hard to study with crystallographic techniques. We have studied the biologically active oligomer of E. Coli ClpB in both nucleotide bound and apo states by using negative stain and cryo electron microscopy techniques. We used image analysis and single particle reconstruction teclmiques to obtain 3 dimensional reconstructions at intermediate resolution of the biological oligomer under both sets of conditions. This reveals E. Coli ClpB forms a two layered heptameric ring with a large cavity in the centre. These particles are asymmetric at resolutions better than ~ 20 A suggesting that ClpB follows sequential or random ATP turnover kinetics. _.' Fitting X-ray crystal structures to the ClpB reconstructions shows that ClpB undergoes large domain movements during its active cycle and provides support for a threading mechanism of unfolding and disaggregation.
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Langhorst, Hanna [Verfasser]. "Deletion of Clmp in Mice Reveals Essential Roles for CLMP in Growth, Survival, Gastrointestinal and Urinary Tract Functions / Hanna Langhorst." Berlin : Freie Universität Berlin, 2016. http://d-nb.info/1084634678/34.
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Tibbles, Katherine L. "Regulation of Clb1 during meiosis in Saccharomyces cerevisiae." Thesis, University of Warwick, 2013. http://wrap.warwick.ac.uk/60444/.
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Meiosis is a specialised form of cell division in which diploid cells divide to form four non-identical spores containing half the genetic complement of the parent. During this cell division program, much of the usual machinery regulating cell division is put to alternate use to allow the cells to undergo an extra round of division without an intervening phase of DNA synthesis. In particular, the end of the first division, meiosis I, must be regulated differently than the end of the mitotic division. We used the model organism Saccharomyces cerevisiae to determine some of these differences in regulation. The cell division program is driven by the sequential association of cyclins with the CDK (cyclin dependent kinase), leading to waves of kinase activity. Exit from mitosis requires the downregulation of CDK activity, and is coordinated by two signalling networks, the FEAR (Cdc14 Early Anaphase Release) network and the MEN (Mitotic Exit Network). Both networks initiate the release of the phosphatase Cdc14 from its inhibitor, Net1, to counter CDK activity. Exit from meiosis I similarly relies on Cdc14 activity, but is driven only by the FEAR network. Experimental results showed that the phosphorylation state and subcellular localisation of the meiotic cyclin, C1b1, are altered in meiosis I. We investigated this relationship and aimed to determine the kinase responsible. We used modelling techniques to explore several rationales for the specific regulation of C1b1. We examined the functional significance of C1b1 localisation, using localisation mutants, and made an investigation into Cdc14 release in meiosis I.
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Mawla, Gina D. (Gina Danielle) Ph D. Massachusetts Institute of Technology. "Functions of alternative ClpP subunits in Pseudomonas aeruginosa." Thesis, Massachusetts Institute of Technology, 2020. https://hdl.handle.net/1721.1/127135.
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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, May, 2020Cataloged from the official PDF of thesis.
Includes bibliographical references.
Proteolysis is the process by which proteins are broken down, or hydrolyzed, into small peptides or amino acids by enzymes. Cells from all forms of life carry out regulated protein degradation as a way to control cellular physiology and regulate stress responses. Clp proteases, containing a AAA+ (A̲TPases A̲ssociated with various cellular A̲ctivities) unfoldase stacked with a compartmentalized peptidase, are central to bacterial proteolysis, and use the energy of ATP hydrolysis to unfold and translocate protein substrates into the peptidase chamber for their destruction. The opportunistic pathogen Pseudomonas aeruginosa is unusual in that it contains two isoforms of the subunits that form the ClpP peptidase chamber. These isoforms, PaClpP1 and PaClpP2, have not been well characterized previously and their specific functions are largely elusive. This work examines the structures and functions of PaClpP1 and PaClpP2 and proposes a model for functional peptides generated by these enzymes in P. aeruginosa development. Biochemical analysis establishes that PaClpP2 is only active as a peptidase when it is part of a PaClpP1₇P2₇ heterocomplex. Furthermore, multiple lines of evidence support that P. aeruginosa cells have two distinct ClpP peptidase assemblies: PaClpP1₁₄ and PaClpP1₇P2₇. Importantly, peptidase and protease analyses establish that these two ClpP assemblies exhibit distinct peptide cleavage specificities and interact differentially with the AAA+ unfoldases, ClpX and ClpA. Finally, the PaClpP2 peptide-cleavage active site uniquely contributes to P. aeruginosa biofilm development. Therefore, results presented in this thesis suggest that within AAA+ proteases, the specificity of the peptidase subunits, not only the recognition properties of the AAA+ unfoldase, control the biological outcome(s) of proteolysis.
by Gina D. Mawla.
Ph. D.
Ph.D. Massachusetts Institute of Technology, Department of Biology
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GAO, XIAOJIANG. "STRENGTH DETERMINATION OF HEAVY CLIP-ANGLE CONNECTION COMPONENTS." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1134401462.
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Compise, Karin D. "Student perceptions of the Clip Chart Management System." Scholarly Commons, 2019. https://scholarlycommons.pacific.edu/uop_etds/3625.
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Six children between the ages of seven and eleven and their parents were interviewed to gather perceptions of the Clip Chart Management System. The Clip Chart system is a behavior system used in many primary classrooms where teachers move students’ clothespins up and down a chart in response to students’ behavior. Some findings from this qualitative case study are: students experienced feelings of shame and embarrassment, students compared their clips to their peers’ clips, and students labeled other students as “bad.” Some parents appreciated the consistency of behavior monitoring, but other parents felt that the system was ineffective and contributed to their child’s negative feelings about school. The findings of this study suggest the need for much more research if this method is continued to be implemented in schools.
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Zheng, Bo. "Characterisation of the Clp Proteins in Arabidopsis thaliana." Doctoral thesis, Umeå : Department of Plant Physiology, Umeå Plant Science Centre, Umeå University, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-99.
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Gersch, Malte [Verfasser]. "Structure, Function and Inhibition of ClpP Proteases / Malte Gersch." München : Verlag Dr. Hut, 2014. http://d-nb.info/1067707883/34.
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Parakh, Neville J. "Design and implementation of a ferry clip test system." Thesis, University of British Columbia, 1989. http://hdl.handle.net/2429/27613.
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In order for Open Systems Interconnection (OSI) to work, protocol implementations must be tested as to their conformance to the specifications they purport to adhere. The International Standards Organization (ISO) has denned a set of abstract test methods for the conformance testing of computer communication protocols. The Ferry Clip concept is a test approach to realize those test methods. It is also a powerful tool that can be used for the diagnostic testing of communication protocols.
This thesis describes the design and implementation of a Ferry Clip based test system in three different environments - UNIX, MPT and OSI-PTE. A method for structuring the system into a specialized set of modules is presented. Such a structuring scheme can considerably reduce the effort required to test different protocol implementations. Implementation issues encountered in building the system under the different hardware/software environments are discussed. The versatility of the Ferry Clip approach is further illustrated by presenting a scheme for its use in Multi-layer and Multi-party testing.Science, Faculty of
Computer Science, Department of
Graduate
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Hersch, Greg Louis. "ClpX interactions with ClpP, SspB, protein substrate and nucleotide." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/34199.
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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, February 2006.Includes bibliographical references.
ClpXP and related ATP-dependent proteases are implements of cytosolic protein destruction. They couple chemical energy, derived from ATP hydrolysis, to the selection, unfolding, and degradation of protein substrates with the appropriate degradation signals. The ClpX component of ClpXP is a hexameric enzyme that recognizes protein substrates and unfolds them in an ATP-dependent reaction. Following unfolding, ClpX translocates the unfolded substrate into the ClpP peptidase for degradation. The best characterized degradation signal is the ssrA-degradation tag, which contains a binding site for ClpX and an adjacent binding site for the SspB adaptor protein. I show that the close proximity of these binding elements causes SspB binding to mask signals needed for ssrA-tag recognition by ClpX. The SspB dimer overcomes this signal masking by tethering itself and bound substrate to ClpX, via docking sites located in the dimeric N-terminal domain of ClpX. Because this N-domain dimer binds only a single SspB subunit, the ClpX hexamer can accommodate just one SspB dimer per hexamer. Other adaptor proteins that use these same tethering sites must compete with SspB for access to ClpXP. Substrates bearing ssrA tags with increased spacing between the SspB and ClpX binding elements are degraded more efficiently at low concentrations by ClpXP.
(cont.) This mechanism in which the adaptor first obstructs and then stimulates substrate recognition may have evolved to permit an additional level of regulation of substrate choice. SspB binding to ssrA-tagged substrate is a highly dynamic process, allowing rapid transfer of substrates from SspB to ClpX. Although the ClpX hexamer is composed of six identical polypeptides, individual subunits assume at least three distinct conformations. Using a hexamer that was engineered to prevent nucleotide hydrolysis, I show that some nucleotide-binding sites in ClpX release ATP rapidly, others release ATP slowly, and at least two sites remain nucleotide free. Occupancy of both the slow sites by ATP and the fast sites by either ATP or ADP is required to bind the degradation tags of protein substrates. The ability of ClpX to retain binding of substrate with ATP or ADP in the fast sites suggests that nucleotide hydrolysis in the fast sites, but not in the slow sites, will allow repeated unfolding attempts without substrate release over multiple ATPase cycles. My results rule out ATPase models including ClpX6eATP6 or ADP6 and also suggest that the enzyme hydrolyzes only a fraction of bound ATP in a single turnover event. Short peptide motifs of ClpX, known as IGF loops, interact with ClpP and change conformation as a response to nucleotide binding by ClpX.
(cont.) As ClpX varies its nucleotide content during the ATP hydrolysis cycle, it also varies its affinity for ClpP. Processing of substrates is coupled to the ATP-hydrolysis cycle of ClpX and appears to modulate ClpX's affinity for ClpP by changing how long each ClpX subunit spends in each nucleotide state.
by Greg Louis Hersch.
Ph.D.
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Zhang, Ting. "Mechanism of aggregate reactivation by the molecular chaperone CLPB." Diss., Kansas State University, 2012. http://hdl.handle.net/2097/13628.
Full textAbstract:
Doctor of PhilosophyGraduate Biochemistry Group
Michal Zolkiewski
ClpB, a bacterial chaperone that belongs to the AAA+ protein family, cooperates with the Hsp70/40 system (DnaK, DnaJ and GrpE in E.coli) in the reactivation of aggregated substrates by translocating them through the central channel of its hexameric form. ClpB is essential for survival of bacteria under heat shock and plays an important role in the infectivity of pathogenic microorganisms. However the detailed mechanism of ClpB disaggregation activity is still not clear. ClpB is a multi-domain protein, which consists of two nucleotide binding domains (NBD1 and NBD2) connected by the middle domain (M domain), and the N-terminal domain connected to the rest of the protein by a flexible linker. In this work, mutations were introduced into the linker region to modify the mobility of the N-terminal domain. It was found that without altering the proper folding and oligomerization of ClpB, all the mutants had deficiencies in aggregate reactivation, possibly due to the weaker binding to aggregated substrates in the initial step of disaggregation. This led to the conclusion that the flexible attachment of the N-terminal domain supports substrate binding and controls the disaggregation by ClpB. Moreover, partial inhibition of the ClpB chaperone activity was observed for all the linker variants, suggesting that the linker sequence might have been optimized by selective pressure to maintain the optimal efficiency of aggregate reactivation. To study the substrate translocation of ClpB, a BAP (ClpB-ClpA P-loop) variant that binds to the protease ClpP was constructed. A FRET-based experiment was designed and the fluorescently-labeled ClpB substrates were produced. This work sets the stage for further studies on the mechanism of aggregate recognition by ClpB. ClpB also plays important roles in pathogenic bacteria invasion and virulence. Recombinant ClpB from Ehrlichia chaffeensis, a pathogenic bacterium that causes human monocytic ehrlichiosis, was purified to study its biochemical properties. Ehrlichia ClpB (Eh_B) and E.coli ClpB (Ec_B) sequences are highly conserved in the nucleotide binding region and poorly conserved in the N-terminal and M domain. The oligomerization, ATPase activity, chaperone activity and substrate binding of the recombinant Eh_B were tested. Recombinant Eh_B was able to reactivate aggregated proteins in the presence of HSP70 from E.coli with equal efficiency as Ec_B. However, the mechanism of Eh_B interactions with substrates and/or substrate specificity may be different from that of E. coli ClpB.
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Martins, Pedro Filipe M. V. C. "Pesquisa de clip arts combinando imagens raster e vectoriais." Master's thesis, Faculdade de Ciências e Tecnologia, 2012. http://hdl.handle.net/10362/8450.
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Existe actualmente um crescente desenvolvimento de sistemas de armazenamento
e pesquisa de imagens. Uma aproximação adoptada nesses sistemas é a recuperação de imagens baseada em conteúdo (CBIR, Content-Based Image Retrieval).
No âmbito destas aplicações existem utilizadores que pretendem utilizar
imagens clip art para os seus trabalhos e apresentações.
Existem muitas imagens clip art espalhadas por diversas bases de dados em sítios na Internet ou em colecções vendidas em dispositivos ópticos. A pesquisa de imagens nestas bases de dados leva os utilizadores a percorrem várias listas de imagens manualmente ou por métodos de pesquisa por texto, muitas vezes ineficientes. Essas bases de dados de clip arts são representadas por imagens vectoriais e imagens raster.
Existem várias tecnologias de pesquisa e recuperação de ambos os tipos de imagens clip art, raster e vectoriais, contudo, a investigação tem sido realizada em separado sem retirar partido das duas áreas de investigação em conjunto, no problema de recuperar e explorar colecções de clip arts. O objectivo deste trabalho é implementar um motor de busca para encontrar clip arts em base de dados compostas por imagens vectoriais e imagens raster. O trabalho envolve um conversor de imagens raster em vectoriais, a extracção de características das imagens raster e vectoriais e a avaliação do sistema de recuperação de clip arts.Fundação para a Ciência e Tecnologia - Projecto CRUSH (Clip art Retrieval using Sketches),referência PTDC/EIA-EIA/108077/2008
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Marchiset-Leca, Dominique. "Pirarubicine : dosages par clhp ; etude pharmacocinetique-pharmacodynamie, applications cliniques." Aix-Marseille 2, 1996. http://www.theses.fr/1996AIX22953.
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Fowler, Shaunda Lynn. "Clip reactions in standing seam roofs of metal buildings." Diss., Mississippi State : Mississippi State University, 2001. http://library.msstate.edu/etd/show.asp?etd=etd-07132001-151614.
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Swain, Martin T. "Protein side-chain placement using CLP." Thesis, University of Aberdeen, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.248614.
Full textAbstract:
Constraint logic programming (CLP) techniques can be used in protein side-chain placement, an important sub-task in comparative modelling. In a simple formulation values for domain variables represent rotamer side-chain conformations, and constraints represent atomic clashes. These constraints can be visualised using a "rotamer contact map", and observations made with this visualisation tool have been used to develop a strategy that overcomes limitations present in CLP caused by over-constrained residues. Null rotamers provide a mechanism that can automatically identify over-constrained residues. The use of null rotamers makes possible an iterative modelling strategy where, at each iteration, a CLP program is generated automatically; each program representing successively tighter packing constraints corresponding to larger atomic radii. Different CLP enumeration heuristics have been evaluated for use with this side-chain placement method, and it has been tested with several different rotamer libraries; a backbone-dependent rotamer library, when used with first-fail enumeration heuristics, was shown to be the most successful. Side-chain conformations predicted by this CLP method compare favourably against those predicted using other side-chain placement methods. The CLP method has been applied to two modelling problems. The first involved building models of class II MHC molecules in order to increase the utility of a peptide threading program. This program uses an allele's known or modelled 3D structure with a heuristic scoring function to predict peptides that are likely to bind to it - thus using CLP to model class II MHC alleles increases the program's utility. The second application used the CLP method to build structures of ribosome inactivating proteins (RIPs). These models were built using CLP together with comparative modelling approaches, and a model of bouganin, a recently identified wild RIF protein, has been built to help design engineered therapeutic proteins.
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Ali, Arslan Mehmet. "Generation and Bioinformatic Analysis of Synthetic Ago HITS-CLIP Data." Thesis, Uppsala universitet, Institutionen för informationsteknologi, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-204891.
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Micro-RNAs (miRNAs) have been discovered to regulate messenger RNA (mRNA) translation and degradation. Various recent studies have been focused on miRNA target prediction, in order to get a better understanding of the rules and nature of miRNA regulation over mRNAs. In this project we aim to create a software module to identify miRNA target sites on mRNAs. As basis to this project, we refer to a study that identified a platform for miRNA-mRNA interaction in protein-RNA complexes in mouse brain (AGO HITS-CLIP study). We propose a probabilistic model of the data from this study, and generate synthetic sample data according to this model, in order to create a test bed for a discovery module. Our discovery module analyzes the sample data to identify peak regions where the interaction density is high. We present results both on synthetic sample data and data from the AGO HITS-CLIP study to evaluate our module.
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Personne, Yoann. "Role of the two ClpP protease subunits in Mycobacterium tuberculosis." Thesis, Queen Mary, University of London, 2012. http://qmro.qmul.ac.uk/xmlui/handle/123456789/2962.
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Caseinolytic (Clp) proteases are the most widespread energy-dependent proteases in bacteria. They are involved in protein quality control by degrading misfolded and aggregated proteins and have a role in regulatory proteolysis. The main group of substrates of the Clp proteases is the SsrA-tagged proteins, which arise in the presence of defective translation. SsrA tagging is carried out by tmRNA, encoded by ssrA, together with a protein partner SmpB. While most organisms have only one ClpP, Mycobacterium tuberculosis has two ClpP protease subunits (ClpP1 and ClpP2) with at least one of them essential for growth. Co-expression of clpP1 and clpP2 was demonstrated showing that clpP1 and clpP2 are not expressed under different conditions. The promoter region of clpP1P2 was identified, together with the potential ClgR binding site. A reporter system to assay ClpP1 and ClpP2 enzymatic activities was developed based on LacZ incorporating SsrA tag sequences. This showed that both ClpP1 and ClpP2 degrade SsrA-tagged LacZ, whilst only ClpP2 degrades untagged proteins. This suggests different pattern recognition for the two ClpP proteins with substrate recognition by ClpP1 dependent on the last three residues of the C-terminus of the tag sequence. Mutagenesis analysis of the accessory components demonstrated that ssrA is essential but SmpB deletion is viable. SmpB is not required for aerobic growth but the smpBΔ mutant strain was more sensitive to antibiotics targeting the ribosome as compared to wildtype cells.
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Cahagne-Leroux, Isabelle. "Etude de l'énantiosélectivité en CLHP de protéines greffées sur silice." Bordeaux 1, 1990. http://www.theses.fr/1990BOR10542.
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Le greffage sur silice diol activee des proteines comme l'albumine de serum humain l'ovalbumine, la chymotrypsine, la mucine, la myoglobine suivant le procede in situ a permis de realiser des colonnes enantioselectives. L'activite de ces proteines est sensible aux facteurs d'environnement tels que la force ionique, le ph et le pourcentage de modificateur organique. L'influence de ces parametres sur la retention, la stereoselectivite et l'efficacite a ete etudiee. Une serie de onze amino-acides a ete chromatographiee a l'aide de differentes phases mobiles: un tampon de phosphate de potassium a des concentrations variees, un ph de 5,8 a 8,0 et l'addition d'un modificateur organique de 0 a 15%. L'ampleur de l'effet de la composition de la phase mobile sur les parametres etudies depend de la structure du solute et de la proteine. Les resultats sont discutes et des applications a la separation de medicaments sont montres
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Jampel, Michael Benjamin. "Over-constrained systems in CLP and CSP." Thesis, City University London, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.319633.
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Rhod, Eduardo Luis. "Quaternary CLB a falul tolerant quaternary FPGA." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2012. http://hdl.handle.net/10183/72925.
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A diminuição no tamanho dos transistores vem aumentando cada vez mais o número de funções que os dispositivos eletrônicos podem realizar. Apesar da diminuição do tamanho mínimo dos transistores, a velocidade máxima dos circuitos não consegue seguir a mesma taxa de aumento. Um dos grandes culpados apontados pelos pesquisadores são as interconexões entre os transistores e também entre os componentes. O aumento no número de interconexões dos circuitos traz consigo um significativo aumento do cosumo de energia, aumento do atraso de propagação dos sinais, além de um aumento da complexidade e custo do projeto dos circuitos integrados. Como uma possível solução a este problema é proposta a utilização de lógica multivalorada, mais especificamente, a lógica quaternária. Os dispositivos FPGAs são caracterizados principalmente pela grande flexibilidade que oferecem aos projetistas de sistemas digitais. Entretanto, com o avanço nas tecnologias de fabricação de circuitos integrados e diminuição das dimensões de fabricação, os problemas relacionados ao grande número de interconexões são uma preocupação para as próximas tecnologias de FPGAs. As tecnologias menores que 90nm possuem um grande aumento na taxa de erros dos circuitos, na lógica combinacional e sequencial. Apesar de algumas potenciais soluções começara a ser investigadas pela comunidade, a busca por circuitos tolerantes a erros induzidos por radiação, sem penalidades no desempenho, área ou potência, ainda é um assunto de pesquisa em aberto. Este trabalho propõe o uso de circuitos quaternários com modificações para tolerar falhas provenientes de eventos transientes. Como principal contribuição deste trabalho destaca-se o desenvolvimento de uma CLB (do inglês Configurable Logic Block) quaternária capaz de suportar eventos transientes e, na possibilidade de um erro, evitá-lo ou corrigi-lo.The decrease in transistor size is increasing the number of functions that can be performed by the electronic devices. Despite this reduction in the transistors minimum size, the circuit’s speed does not follow the same rate. One of the major reasons pointed out by researchers are the interconnections between the transistors and between the components. The increase in the number of circuit interconnections brings a significant increase in energy consumption, propagation delay of signals, and an increase in the complexity and cost of new technologies IC designs. As a possible solution to this problem the use of multivalued logic is being proposed, more specifically, the quaternary logic. FPGA devices are characterized mainly by offering greater flexibility to designers of digital systems. However, with the advance in IC manufacturing technologies and the reduced size of the minimum fabricated dimensions, the problems related to the large number of interconnections are a concern for future technologies of FPGAs. The sub 90nm technologies have a large increase in the error rate of its functions for the combinational and sequential logic. Although potential solutions are being investigated by the community, the search for circuits tolerant to radiation induced errors, without performance, area, or power penalties, is still an open research issue. This work proposes the use of quaternary circuits with modifications to tolerate faults from transient events. The main contribution of this work is the development of a quaternary CLB (Configurable Logic Block) able to withstand transient events and the occurrence of soft errors.
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Dominique, Manon. "Effets pharmacologiques d'une protéine bactérienne mimétique d'hormones satiétogènes : la protéine ClpB sur le comportement alimentaire." Thesis, Normandie, 2019. http://www.theses.fr/2019NORMR072/document.
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L’étude du microbiote intestinal et de ses produits de sécrétion est un domaine de recherche en expansion en raison des perspectives thérapeutiques que cela peut ouvrir pour les maladies nutritionnelles telles que l’obésité ou les TCA. La Caseinolytic peptidase B (ClpB) est une protéine bactérienne produite par les entérobactéries qui présente un mimétisme moléculaire avec l’α-MSH, un neuropeptide anorexigène signalant la satiété au niveau de l’hypothalamus. Des études récentes ont cherché à évaluer si la protéine ClpB pouvait induire des effets anorexigènes similaires à ceux de l’α-MSH, en situation physiopathologique et dans des modèles de troubles nutritionnels. Dans ce contexte, l’objectif de cette thèse a été d’étudier les potentiels effets pharmacologiques de la protéine ClpB, intacte ou fragmentée sur les différents mécanismes de régulation de la prise alimentaire impliquant l’axe microbiote-intestin-cerveau et l’influence des nutriments. La première étude a évalué in vitro l’impact de trois macronutriments sur la production et l’expression de la protéine ClpB par les bactéries E. coli : seul l’apport protéique augmentait significativement la production de ClpB. Nous avons montré que la ClpB augmentait la sécrétion de PYY par les cellules entéro-endocrines intestinales de rat en culture. La deuxième d’étude a été réalisée chez des souris dans un modèle d’anorexie (ABA). La restriction alimentaire, avec ou sans activité physique, augmentait la concentration plasmatique de ClpB, qui était associée à une augmentation de la proportion d’entérobactéries dans le microbiote, ce qui suggère son implication possible dans la physiopathologie de l’anorexie mentale. Enfin, la troisième étude a évalué in vivo chez le Rongeur, les effets pharmacologiques de la protéine ClpB sur la prise alimentaire. La prise alimentaire était réduite par l’injection de ClpB intacte ou fragmentée, mais pas par son fragment de 25 kDa. Ces résultats confortent le rôle de la protéine ClpB, produite par les entérobactéries, dans la régulation physiologique de la prise alimentaire et incitent à poursuivre l’étude de son implication dans les troubles anorexiques et son application thérapeutique dans les situations d’excès de poidsThe study of the gut microbiota and especially the effect of its secretory products is an expanding field of research in order to open therapeutic perspectives for nutritional diseases such as obesity or TCA. Among these molecules, the Caseinolytic peptidase B (ClpB) is a bacterial protein having a molecular mimicry in common with α-MSH, a neuropeptide whose anorectic actions are peripheral and central and possible via a microbiota-intestinal-brain communication. Current studies attempt to demonstrate whether this molecular mimicry can confer similar anorectic effects at the ClpB protein. The aim of this thesis was studied the potential pharmacological effects of ClpB protein the regulation of eating behavior. Given that the composition of the gut microbiota is dependent on the food present, the first study was evaluated in vitro the impact of three types of macronutrients on the production and expression of the ClpB protein by E. coli bacteria. Then, it was evaluated whether this protein could influence the secretion of satietogenic peptides like PYY by intestinal enteroendocrine cells using a primary culture of rat intestinal cells. Previous studies of U 1073 laboratory have shown that this protein has been found at the plasma level, the second study was performed in mice submitted to an anorexia model (ABA) to clarify the impact of dietary restriction on the ClpB protein, to better understand its possible involvement in the physiopathology of anorexia nervosa. Finally, the third study was evaluated the pharmacological effects of ClpB protein on food intake in vivo in rodents. The impact of the natural fragmentation of this protein and particularly of one of its fragments on food intake was also evaluated
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Magnusson, Jonna. "Lifeloggingkamera som hjälpmedel för familjer där ett barn har autism: En utvärdering av Narrative Clip : En kvalitativ studie hur Narrative Clip kan användas som ett hjälpmedel." Thesis, Linköpings universitet, Institutionen för datavetenskap, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-110129.
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Studien ämnar att testa ett nytt användningsområde för Narrative Clip. Syftet med studien var att undersöka potentiell nytta eller möjliga problem som användandet av Narrative Clip kan medföra till familjer som har barn med autism. För att undersöka detta har fyra familjer deltagit i studien, de har fått låna en kamera i två veckor. Under dessa två veckor fick deltagarna använda kameran på det sätt som passade deras behov. Efter att deltagarna använt kameran i två veckor genomfördes intervjuer med föräldrarna. Det kvalitativa materialet analyserades med hjälp av IPA för att finna deltagarnas upplevelse kring användandet utav kameran. Analysen resulterade i både många positiva men samt några negativa aspekter av att använda kameran som ett hjälpmedel.
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Liu, Zhonghua. "Characterization of two AAA+ proteins : Escherichia coli ClpB and human torsina /." Search for this dissertation online, 2005. http://wwwlib.umi.com/cr/ksu/main.
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Raschperger, Elisabeth. "Studies on CAR and CLMP, two proteins of epithelial tight junctions /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-909-2/.
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Polavarapu, Anjaneya Prasad. "Exploring Molecular Interactions : Synthesis and Studies of Clip-Shaped Molecular Hosts." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-8220.
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Lee, Mary Elizabeth Ph D. Massachusetts Institute of Technology. "Regulation of ClpP : role of substrate gating and activation by ClpX." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/58374.
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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2010."January 2010." Cataloged from PDF version of thesis.
Includes bibliographical references.
AAA+ self-compartmentalized proteases are an important class of proteome regulators that operate to selectively degrade protein substrates. All of these enzymes share the architectural theme of a hexameric ring unfoldase stacked axially onto a barrel-like peptidase, with six- or seven-fold symmetry and sequestered active sites. ClpXP is a model self-compartmentalized protease composed of the regulator ClpX and the serine protease ClpP. Proteolysis occurs by ClpX-dependent substrate selection, unfolding, and translocation into the degradation lumen of ClpP, where rapid and relatively non-specific peptide hydrolysis generates small peptide products. Prior work had shown that ClpP is unable to degrade polypeptides in the absence of ClpX, suggesting the existence of a mechanism that inhibits the activity of free ClpP. Structures of free ClpP show active sites geometrically competent to perform peptide-hydrolysis chemistry. However, some biochemical results suggested that N-terminal ClpP residues, which line the axial entrance pores, allosterically regulate these active sites. Through measurements of ClpP active-site reactivity, degradation of size-varied peptides, and mutagenesis of the N-termini, I found that peptide degradation is inhibited by steric occlusion, maintained by the N-terminal 3-stem loop and a-helix A of ClpP. The N-termini also participate in specifying substrate choice, as mutations within the axial channel prevent degradation of peptides containing stretches of charged amino acids. These data support a model in which ClpX binding opens the axial pore of ClpP to facilitate polypeptide translocation.
(cont.) Additional residues in ClpP that are important for its function were identified by a selection for dominant-negative mutants impaired in ClpXP-dependent proteolysis. Biochemical studies and mapping of these mutations onto the structure of ClpP suggest that these variants are defective in tetradecamer assembly, peptide binding at the active sites, and ClpX binding. This work provides a foundation for further investigations of the mechanisms of ClpP assembly, degradation, and interactions with ClpX.
by Mary Elizabeth Lee.
Ph.D.
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Nuez, Catherine. "Méthodes d'analyse en microdialyse cérébrale et sanguine par CLHP-SM-SM." Mulhouse, 1996. http://www.theses.fr/1996MULH0428.
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La microdialyse est un excellent outil pour définir la concentration de substances directement dans leur site d'action, à condition d'utiliser une méthode analytique suffisamment sensible et spécifique pour l'analyse de faibles quantités venant des faibles volumes de dialysats disponibles (25 à 60 microlitres, en général). Cette méthode analytique doit être suffisamment efficace pour déterminer la pharmacocinétique de médicaments. Les méthodes analytiques très courantes (CLHP-UV ou CLHP-détection électrochimique) ne sont pas toujours appropriées pour l'analyse de microdialysats, de par leur manque de sensibilité et surtout de spécificité. Le but du présent travail est de pallier cet inconvénient majeur en développant des méthodes analytiques sensibles et spécifiques par chromatographie liquide couplée à la spectrométrie de masse en tandem (peu utilisées jusqu'à présent dans ce domaine). Dans notre travail, deux types de microdialyse et trois composés sont utilisés : la microdialyse cérébrale dans le cortex frontal pour quantifier le passage des substances -W, Z et ENA- à travers la barrière hématoencéphalique ; la microdialyse sanguine dans la veine porte et la veine jugulaire afin d'étudier l'effet de premier passage intestinal ou hépatique de ENA. La détermination rapide de la concentration des échantillons de microdialyse est possible grâce à la chromatographie liquide couplée à la spectrométrie de masse en tandem par une interface thermospray (CLHP-TSP-SM-SM) pour les composés W et Z, et grâce à la spectrométrie de masse en tandem en mode injection directe, c'est à dire sans colonne chromatographique, pour ENA et son métabolite (MET). Ces deux méthodes analytiques sont optimisées de façon à obtenir le maximum de sensibilité et de spécificité pour chacune des substances à étudier. Quelle que soit la méthode utilisée, la réponse des composés W, Z, ENA et MET est linéaire avec d'excellentes limites de quantification (LOQ). La combinaison de telles méthodes avec la microdialyse, associée à la méthode de rétrodialyse pour le calcul du rendement des sondes de microdialyse, indique une pénétration cérébrale significative de Z et ENA, un passage de W à travers la barrière hématoencéphalique plus difficile, un effet de premier passage hépatique de ENA non négligeable et apparemment une métabolisation de ENA au niveau pré-hépatique.
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Eriksson, Marcus. "A CLP(FD)-based model checker for CTL." Thesis, Linköping University, Department of Computer and Information Science, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-109.
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Model checking is a formal verification method where one tries to prove or disprove properties of a formal system. Typical systems one might want to prove properties within are network protocols and digital circuits. Typical properties to check for are safety (nothing bad ever happens) and liveness (something good eventually happens).
This thesis describes an implementation of a sound and complete model checker for Computation Tree Logic (CTL) using Constraint Logic Programming over Finite Domains (CLP(FD)). The implementation described uses tabled resolution to remember earlier computations, is parameterised by choices of computation strategies and can with slight modification support different constraint domains. Soundness under negation is maintained through a restricted form of constructive negation.
The computation process amounts to a fixpoint search, where a fixpoint is reached when no more extension operations has any effect. As results show, the choice of strategies does influence the efficiency of the computation. Soundness and completeness are of course independent of the choice of strategies. Strategies include how to choose the extension operation for the next step and whether to perform global or local rule instantiations, resulting in bottom-up or top-down computations respectively.
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Asplund, Mikael. "En optimierande kompilator för SMV till CLP(B)." Thesis, Linköping University, Department of Computer and Information Science, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-2805.
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This thesis describes an optimising compiler for translating from SMV to CLP(B). The optimisation is aimed at reducing the number of required variables in order to decrease the size of the resulting BDDs. Also a partitioning of the transition relation is performed. The compiler uses an internal representation of a FSM that is built up from the SMV description. A number of rewrite steps are performed on the problem description such as encoding to a Boolean domain and performing the optimisations.
The variable reduction heuristic is based on finding sub-circuits that are suitable for reduction and a state space search is performed on those groups. An evaluation of the results shows that in some cases the compiler is able to greatly reduce the size of the resulting BDDs.
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Strub, Christine Eva. "Untersuchungen zur Struktur und Substratspezifität des AAA+-Chaperons ClpB in Escherichia coli." [S.l.] : [s.n.], 2006. http://deposit.ddb.de/cgi-bin/dokserv?idn=979900379.
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Dany, Hendra. "Application of the ferry clip approach to multi-party and interoperability testing." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/28971.
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As communications protocols are becoming more complex and sophisticated, developing
a test system that has the ability to provide a controlled environment for comprehensive
protocol testing is essential to achieve a "real open system". This thesis advocates the need for a multi-party test method as currently identified by ISO, and discusses two important aspects of protocol testing: Conformance and Interoperability. They are complementary to each other and are necessary to ensure the conformity and interoperability
of a protocol implementation. The proposed ferry clip based test architecture is presented. Both the concepts and design principles employed to achieve a flexible and generalized test system and the specific components which comprise the Ferry Clip based Test System are described. The test system is general and flexible not only with respect to the test configurations and test methods but also with respect to the protocol to be tested, the system under test, and the underlying communication system. Applications of the ferry clip approach to multi-party conformance and interoperability testing are discussed, followed by an example of MHS conformance testing which demonstrates the applicability of the ferry clip approach to multi-party testing.Science, Faculty of
Computer Science, Department of
Graduate
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Lyons, Robert Joseph 1963. "Determining distributed source waveforms in casual, lossy, dispersive, plane-wave (CLDP) materials." Thesis, Massachusetts Institute of Technology, 1998. http://hdl.handle.net/1721.1/47722.
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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1998.Includes bibliographical references (p. 284-291).
This thesis presents and employs novel mathematics for the inversion of linear, first-kind Fredholm integral equations (IEs) which have a time t dependent response signal, a space z dependent source waveform, and a kernel with time dependence (at each z) corresponding to the impulse response of a thickness z slab of causal, lossy, dispersive, homogeneous material through which planar disturbances propagate according to the wave equation. These materials are called CLDP materials; these IEs are called CLDP IEs. These novel mathematics are applicable to the PESAW (aka PEA) charge recovery method. The proposed inversion method recognizes that the (temporal) Fourier transform of a CLDP IE's response signal can be interpreted as the values of the (spatial) Laplace transform of that IE's source waveform along a Laplace plane path determined by the material's propagation wavenumber k(f). Executing the Laplace transform inversion integral along this CLDP path yields an inverse CLDP IE which recovers the true source waveform provided that source waveform is real, causal, Fourier-transformable, and also satisfies the proposed k(f)-dependent 'CLDP criterion'. The forward and inverse CLDP IEs corresponding to a particular CLDP material model k(f) therefore comprise a particular integral transform relationship applicable to waveforms satisfying the CLDP criterion for that material. The CLDP transform relationship for a lossless/dispersionless material reduces to the (unilateral) Fourier transform. Even without noise, the 'inverse CLDP'-recovered waveform gleaned from an abruptly bandlimited CLDP response signal requires regularization - a generalized Gibbs-Dirichlet kernel dubbed 'the Darrell' comes into effect. The measured (time sampled) PESAW signal is necessarily bandlimited; this thesis investigates regularization via lowpass filtering of the measured signal. Both synthetic and experimental examples are investigated. The focus is on MHz-range signals culled from mm-range polymeric PESAW experiments. A method for determining the requisite model k(f) from measured PESAW signals is also presented and employed.
by R. Joseph Lyons.
Ph.D.
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Nolasco, Eduardo Lima. "Estudo da sepse experimental em animais diabéticos e sadios, tratados ou não com insulina." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-03052013-143917/.
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Sepse e choque séptico são causas frequentes de morte nas unidades de terapia intensiva, sendo responsável pelo alto custo global de internação, segundo DATA-SUS. Sabendo-se que o paciente diabético apresenta um maior risco de infecção e que 22% dos indivíduos com sepse apresentam diabetes, é de extrema importância o melhor entendimento da sua fisiopatologia no paciente diabético para que medidas intervencionistas sejam desenvolvidas, preservando vidas. O projeto buscou avaliar a sepse experimentalmente através do modelo de ligadura e perfuração do ceco (CLP - 2 perfurações) em ratos Wistar machos sadios e tornados diabéticos através da injeção endovenosa de aloxana (42 mg/kg, i.v., 10 dias). Além disso, buscamos avaliar se o tratamento com insulina alteraria as variáveis escolhidas. O trabalho avaliou parâmetros hematológicos e bioquímicos como uréia, creatinina, alanina aminotransferase (ALT), aspartato aminotransferase (AST) e fosfatase alcalina (FAL). Após 6 horas da realização da CLP foram coletados lavados broncoalveolares (LBA) e peritoneal (LPe) com o objetivo de estudar o perfil de citocinas através da dosagem das interleucinas (IL)-1β, IL-6, IL-10, fator de necrose tumoral (TNF)-α e cytokine-induced neutrophill chemoattractant (CINC)-1 e CINC-2, celularidade total e específica. Rim, pulmão e fígado foram coletados para análises morfológicas e da atividade da mieloperoxidase (MPO). Após 6 horas de CLP observamos que a hematimetria, hemoglobina, plaquetometria e celularidade do LBA não sofreram alterações nem após o tratamento com insulina. A sepse provocou diminuição da leucometria total nos animais diabéticos e controles, aumento da celularidade total do LPe com um predomínio de polimorfonucleares, que foi semelhante em ambos os grupos; aumento da concentração no LPe de IL-1β, IL-6, CINC-1, CINC-2 e IL-10; as concentrações do TNF-α permaneceram idênticas em ambos os grupos. Em relação aos marcadores hepáticos, foram observados que os animais diabéticos possuem os maiores valores de ALT, AST e FAL em relação ao grupo controle e já apresentavam uma disfunção morfológica de hepatócitos, porém, sem infiltrado neutrofílico. O tratamento com insulina reduziu os valores dessas enzimas em níveis próximos aos do controle. Dos marcadores renais, apenas a uréia sofreu aumento significativo nos animais diabéticos em relação aos controles, sendo exacerbada pela CLP. Alterações glomerulares, como capilares dilatados e redução do espaço de Bowman foram observadas nos animais com sepse, os quais não sofreram influência após o tratamento com insulina. Estes dados sugerem que a insulina teve um efeito hepato-protetor e que o padrão causado pela sepse nos animais controles e diabéticos não foi modificado pelo tratamento com insulina.Sepsis and septic shock are common cause of death in intensive care units (ICU) and according to Brazilian DATA-SUS is one of the major causes of high cost hospitalization. Since diabetic patients have a higher risk of infection and represent approximately 22% of all the septic individuals, it is extremely important to understand the pathophysiology of sepsis in diabetic patients in order to develop interventional measure. The objective of this study is to evaluate the experimental sepsis model by cecal ligation and puncture (CLP 2 punctures) in Wistar healthy males rendered diabetic by intravenous injection of alloxan (42 mg/kg, i.v., 10 days). The study analyzed hematological and biochemical parameters such as urea, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). Furthermore, we studied how insulin treatment could modulate these parameters. After 6 hours of CLP bronchoalveolar (BAL) and peritoneal lavages (PeL) were collected and a cytokine profile was accessed: interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α, cytokine-induced neutrophill chemoattractant (CINC)-1, CINC-2. Samples from kidney, lung and liver were collected for morphological analysis and for the measurement of myeloperoxidase activity (MPO). After 6 hours of CLP, red blood cell count, hemoglobin, platelet count, cytokine profile and cellularity of the BAL did not change. In addition, insulin treatment did not change these parameters. CLP caused a decrease in total leukocyte count in both groups diabetic and control rats. Moreover, this model induced a rise in total PeL cellularity with a predominance of polymorfonuclear cells, which was similar in both groups control and diabetic rats. In the PeL, proinflammatory cytokines such as IL-1β, IL-6, CINC-1, CINC-2 and the antinflammatory cytokine IL-10 were enhanced. The TNF-α concentrations remained similar in both groups. Hepatic markers inferring in hepatocyte dysfunction were higher in the diabetes-induced animals what was in part restored after insulin administration. Regarding renal markers, only urea levels were enhanced in diabetic rats and worsened after CLP induction. Morphological changes, such as capillary dilatation and reduced Bowman space was already observed in the kidney of animals with sepsis, insulin treatment did not corrected the values. These data suggest that insulin had a hepato-protective effect but further changes were not observed after insulin treatment.
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Salas, Erika. "Les pigments du vin rouge : étude des réactions directes entre anthocyanes et flavanols." Montpellier, ENSA, 2005. http://www.theses.fr/2005ENSA0009.
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La couleur des vins rouges est imputable aux anthocyanes extraites à partir du raisin mais aussi aux pigments formés à partir de ces molécules, notamment par réaction avec les flavanols. Les anthocyanes réagissent avec les flavanols, soit directement, soit par des voies impliquant aussi l'acétaldéhyde. L'objectif de cette étude était de déterminer les mécanismes des réactions directes, ainsi que la structure et les propriétés colorantes des produits qui en sont issus. Ces réactions ont été suivies en solutions modèles par chromatographie liquide haute performance couplée à la spectrophotométrie UV-visible et à la spectrométrie de masse. Deux groupes de pigments, flavanols-anthocyanes (F-A) et anthocyanes-flavanols (A-F), ont été mis en évidence. Les premiers, qui résultent de l'addition des anthocyanes sur les espèces intermédiaires formées par clivage acido-catalysé des proanthocyanidines, ont été obtenus par hémisynthèse, isolés par chromatographie à contre courant et identifiés par RMN. Leurs propriétés colorantes sont identiques à celles de l'anthocyane. L'application des techniques développées pour l'analyse des milieux modèles à un vin a permis d'isoler une fraction colorée polaire et d'y mettre en évidence, en plus des produits isssus des réactions avec l'acétaldéhyde, deux groupes d'adduits résultant de réactions directes: les pigments F-A mais aussi des produits A-F incolores. L'importance relative des adduits A-F et F-A est dépendante du pH, la première étape conduisant aux F-A étant favorisée en milieu acide. Les mécanismes de formation des adduits A-F colorés et incolores et l'influence des trois types d'adduits sur la qualité des vins restent à élucider.
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Atanasova, Vessela Dimitrova. "Réactions des composés phénoliques dans les vins rouges induites par la technique de micro-oxygénation : caractérisation de nouveaux produits de condensation des anthocyanes avec l'acétaldéhyde." Montpellier, ENSA, 2003. http://www.theses.fr/2003ENSA0002.
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Dans cette étude, l'influence de la micro-oxygénation sur la composition phénolique et les caractéristiques colorimétriques d'un vin a été étudiée. Des changements significatifs entre les vins oxygénés et témoins ont eu lieu à partir du septième mois de conservation. L'analyse en Composantes Principales appliquée sur les vins à différents stades de leur vieillissement a montré clairement que les vins sont séparés, le long du premier axe, en fonction de leur temps de conservation, et le long du second axe, en fonction de l'oxygénation. Avec le temps, la concentration des anthocyanes natives a progressivement diminué et des structures plus stables telles que les pyranoanthocyanes et les pigments T-A+ se sont accumulées. La micro-oxygénation a favorisé les mécanismes impliquant l'acétaldéhyde, à savoir les réactions de condensation entre les composés phénoliques et l'acétaldéhyde, et les réactions de cycloaddition entre les anthocyanes, les flavanols et l'acétaldéhyde. Au cours de l'étude de ces mécanismes en solutions modèles, deux nouvelles structures ont été mises en évidence, résultant soit de la réaction directe entre les anthocyanes (Mv 3g-Mv 3g), soit induite par l'acétaldéhyde (Mv 3g-éthyl-Mv 3g). Ce dernier dérivé a été également détecté dans les vins. Compte tenu de l'intérêt que représente cette molécule dans le domaine de l'œnologie sur le plan de la couleur, elle a été purifiée afin d'établir sa structure par RMN et d'effectuer des études sur ses propriétés physico-chimiques. Une fraction oligomérique caractérisée par un DPm de 3,1 a été également isolée. La mise en évidence des oligomères constitués d'unités Mv 3g reliées par des ponts éthyles indique que la position C6 des anthocyanes est également réactiveIn this study, the influence of micro-oxygenation on the phenolic composition and the colour characteristics of a wine were studied. Significant changes between the oxygenated and control wines were observed after seven months of storage. Principal Component Analysis applied to the wines at various stages of ageing showed c1early that the wines are separated, along the first axis, according to their storage time, and along the second axis, according to oxygenation. Over time, the concentration of the native anthocyanins gradually decreased and more stable structures such as pyranoanthocyanins and T-A+ pigments accumulated. Micro-oxygenation favoured the mechanisms involving acetaldehyde, i. E. Condensation reactions between phenolic compounds and acetaldehyde, and reactions of cyc1oaddition between anthocyanins, flavanols and acetaldehyde. During the study of these mechanisms in mode! solutions, two new structures were detected, resulting either from the direct reaction between anthocyanins (Mv 3g-Mv 3g), or induced by acetaldehyde (Mv 3g-ethyl-Mv 3g). The last derivative was also detected in the wines. Taking into account the interest that this molecule presents in the field of enology, it was purified in order to investigate its structure by NMR and to study its physical-chemical properties. An oligomeric fraction characterised by DPm 3,1 was also isolated. The detection of ethyl-linked Mv 3g oligomers indicates that the C6 position of the anthocyanins is also reactive
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Jesus, Aline Alves de. "Hidrogênio molecular inibe a resposta inflamatória e previne o dano cognitivo em ratos submetidos ao choque séptico." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/17/17134/tde-07022019-132708/.
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O sistema nervoso central (SNC) é uma das primeiras regiões a ser acometida durante a sepse e choque séptico, o que contribui para o aumento da taxa de morbidade e mortalidade. Pacientes em choque séptico apresentam disfunção neuronal aguda, tais como o delírio, desorientação e coma. Em longo prazo, o dano cognitivo pode ocorrer ocasionando o comprometimento do aprendizado e formação de memória. Estudos demonstram que durante a resposta inflamatória sistêmica exacerbada, mediadores inflamatórios presentes na circulação sistêmica, são capazes de chegar ao SNC e ocasionar a ativação de células gliais, conduzindo a um estado de neuroinflamação. Nesse processo, algumas estruturas do SNC, tais como o hipocampo são mais vulneráveis à ação de espécies reativas de oxigênio (ERO), e a mediadores inflamatórios produzidos de forma excessiva durante a sepse. Neste contexto, a investigação de novas estratégias terapêuticas que sejam capazes de atenuar a resposta inflamatória exacerbada se faz necessário. Assim, o presente projeto teve como objetivo investigar prováveis propriedades antioxidante e anti-inflamatória do Hidrogênio molecular (H2), bem como sua possível ação neuroprotetora em ratos submetidos à sepse polimicrobiana, induzida por ligadura e perfuração cecal (CLP). Para isso o projeto foi dividido em dois protocolos experimentais. No primeiro protocolo ratos Wistar submetidos à cirurgia de CLP ou Sham, foram submetidos ao tratamento com inalação do H2 a 2%, por um período de 1h durante 10 dias consecutivos, e logo após foram submetidos a testes comportamentais para avaliação da memória de habituação, discriminativa e aversiva. No segundo protocolo os animais foram tratados com inalação do H2 por um período de 3h, e 24h após ao término da cirurgia de CLP/Sham foram decapitados para coleta do sangue e cérebro. A partir dos resultados dos testes comportamentais observamos que o tratamento com inalação do H2 durante a sepse experimental preveniu a perda de memória e o dano cognitivo, bem como foi capaz de diminuir os níveis de citocinas pró-inflamatórias de fase aguda tais como IL-1?, IL-6 e TNF? no córtex pré-frontal e hipocampo. A estratégia também foi capaz de diminuir os níveis de TBARS no plasma. Observamos um aumento da concentração da enzima catalase nos animais tratados com H2. Em conjunto os resultados indicam que o H2 foi capaz de inibir a resposta inflamatória e prevenir o dano cognitivo, agindo como uma substância neuroprotetora em ratos submetidos ao choque séptico experimentalThe central nervous system is one of the first regions to be affected during Sepsis and septic shock, which contributes to the increased rate of morbidity and mortality. Patients with severe sepsis may present acute neuronal dysfunction such as delirium, disorientation, and unconscious. In the long term, cognitive damage can occur causing the commitment of learning and memory formation. Studies show that during the exacerbated systemic inflammatory response, inflammatory mediators present in the systemic circulation, are able to reach the CNS and cause the activation of glial cells, leading to a state of neuroinflammation. In this process, some CNS structures such as the hippocampus are more vulnerable to the action of reactive oxygen species (ROS), and to inflammatory mediators produced excessively during sepsis. In this context, the investigation of new therapeutic strategies that are capable of attenuating the exacerbated inflammatory response is necessary. Thus, the present project aimed to investigate the probable antioxidant and anti-inflammatory properties of molecular hydrogen (H2), as well as its possible neuroprotective action in rats submitted to polymicrobial sepsis induced by ligature and cecal puncture (CLP). In order to check this hypothesis, the project was divided into two experimental protocols. In the first protocol Wistar rats submitted to CLP or Sham surgery were submitted to 2% H2 inhalation treatment for a period of 1h for 10 consecutive days, and soon after they underwent behavioral tests to evaluate habituation memory, discriminative and aversive. In the second protocol the animals were treated with H2 inhalation for a period of 3h and 24h, and at the end of the treatment, they were decapitated for blood and brain collection. Plasma was already used for nitrate dosage, lipid peroxidation, antioxidant enzymes and inflammatory cytokines. From the results of the behavioral tests, we observed that treatment with H2 inhalation during the experimental sepsis prevented memory loss and cognitive damage, and was able to decrease the levels of acute-phase inflammatory cytokines such as IL-1?, IL -6 and TNF? in the prefrontal cortex and hippocampus. The therapeutic strategy was also able to decrease plasma TBARS levels. We also observed an increase in the concentration of the enzyme catalase in H2-treated animals. Together the results indicate that molecular hydrogen was able to inhibit the inflammatory response and prevent cognitive damage, acting as a neuroprotective substance, in rats submitted to experimental septic shock
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Cordelières, Fabrice. "Quelle fonction pour la CLIP-170 ? Recherche de partenaires et nouveaux outils d'investigation." Phd thesis, Université Paris Sud - Paris XI, 2003. http://tel.archives-ouvertes.fr/tel-00432222.
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Le terme de CLIP-170 désigne la protéine de lien cytoplasmique de 170 KDa, isolée par Rickard et Kreis (1990). In vitro, elle constitue un lien statique entre les endosomes et les microtubules (MTs). En chacun des points où la CLIP-170 est localisée, elle se co-distribue avec le complexe moteur dynéine/dynactine (D/D). Les auteurs ont initialement établi un modèle de fonctionnement de concert de ces trois protagonistes : la CLIP-170 établirait le lien initial entre le cargo et le MT. Le complexe D/D serait recruté sur le cargo. Une fois le moteur associé au MT, le lien statique serait levé, rendant possible le mouvement. Ce modèle offrait une explication aux données d'immunolocalisation. Toutefois, le fonctionnement de concert de la CLIP-170 et du moteur moléculaire nécessitait que l'on puisse trouver une interaction entre les protagonistes. Les travaux présentés dans cette thèse apportent pour la première fois la preuve d'une interaction indirecte entre le complexe D/D et la CLIP-170. LIS1 est une protéine codée par le gène causal du syndrome de Miller-Dieker, une forme de lissencéphalie de type I. Elle sert d'adaptateur entre le domaine carboxy-terminal de la CLIP-170 et le complexe moteur dont elle régule l'activité. Nos résultats établissent que le domaine d'interaction de la CLIP-170 avec LIS1 est requis pour l'adressage de la première aux kinétochores prémétaphasiques. Il est nécessaire à l'adressage de LIS1 (et du complexe moteur) aux bouts (+) des MTs interphasiques. Nous présentons deux nouvelles approches permettant d'interférer avec le fonctionnement de la CLIP-170 tant en interphase qu'en mitose : la microinjection d'anticorps dirigés contre les domaines extrêmes de la protéine, ainsi que l'utilisation de siRNA dirigés contre l'ARNm la codant. Combinées aux techniques de suivi de protéines fluorescentes par vidéomicroscopie 3D développées au laboratoire, elles permettront d'interférer avec le fonctionnement de la CLIP-170 et de définir ainsi son rôle.
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Guo, Dong. "Analytical investigation of block shear of coped beams with welded clip angles connection." Thesis, University of Macau, 2007. http://umaclib3.umac.mo/record=b1636330.
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Le, Solleu Hervé. "Le bromazépam : de l'anxiolyse à la toxicité : dosage dans le plasma par CLHP." Bordeaux 2, 1992. http://www.theses.fr/1992BOR2P031.
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