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Journal articles on the topic "CKX7"
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Gasparis, Przyborowski, Kała, and Nadolska-Orczyk. "Knockout of the HvCKX1 or HvCKX3 Gene in Barley (Hordeum vulgare L.) by RNA-Guided Cas9 Nuclease Affects the Regulation of Cytokinin Metabolism and Root Morphology." Cells 8, no. 8 (July 26, 2019): 782. http://dx.doi.org/10.3390/cells8080782.
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Barley is among four of the most important cereal crops with respect to global production. Increasing barley yields to desired levels can be achieved by the genetic manipulation of cytokinin content. Cytokinins are plant hormones that regulate many developmental processes and have a strong influence on grain yield. Cytokinin homeostasis is regulated by members of several multigene families. CKX genes encode the cytokinin oxidase/dehydrogenase enzyme, which catalyzes the irreversible degradation of cytokinin. Several recent studies have demonstrated that the RNAi-based silencing of CKX genes leads to increased grain yields in some crop species. To assess the possibility of increasing the grain yield of barley by knocking out CKX genes, we used an RNA-guided Cas9 system to generate ckx1 and ckx3 mutant lines with knockout mutations in the HvCKX1 and HvCKX3 genes, respectively. Homozygous, transgene-free mutant lines were subsequently selected and analyzed. A significant decrease in CKX enzyme activity was observed in the spikes of the ckx1 lines, while in the ckx3 lines, the activity remained at a similar level to that in the control plants. Despite these differences, no changes in grain yield were observed in either mutant line. In turn, differences in CKX activity in the roots between the ckx1 and ckx3 mutants were reflected via root morphology. The decreased CKX activity in the ckx1 lines corresponded to greater root length, increased surface area, and greater numbers of root hairs, while the increased CKX activity in the ckx3 mutants gave the opposite results. RNA-seq analysis of the spike and root transcriptomes revealed an altered regulation of genes controlling cytokinin metabolism and signaling, as well as other genes that are important during seed development, such as those that encode nutrient transporters. The observed changes suggest that the knockout of a single CKX gene in barley may be not sufficient for disrupting cytokinin homeostasis or increasing grain yields.
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Köllmer, Ireen, Ondřej Novák, Miroslav Strnad, Thomas Schmülling, and Tomáš Werner. "Overexpression of the cytosolic cytokinin oxidase/dehydrogenase (CKX7) from Arabidopsis causes specific changes in root growth and xylem differentiation." Plant Journal 78, no. 3 (April 7, 2014): 359–71. http://dx.doi.org/10.1111/tpj.12477.
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Schwarz, Ireen, Marie-Therese Scheirlinck, Elisabeth Otto, Isabel Bartrina, Ralf-Christian Schmidt, and Thomas Schmülling. "Cytokinin regulates the activity of the inflorescence meristem and components of seed yield in oilseed rape." Journal of Experimental Botany 71, no. 22 (September 10, 2020): 7146–59. http://dx.doi.org/10.1093/jxb/eraa419.
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Abstract The number of flowers and seed-bearing structures formed by the inflorescence meristem and the formation of ovules in the female reproductive part of the flowers are important yield-related traits of crop plants. It has been shown that cytokinin is a pivotal factor regulating these traits. Here, we explore the impact of mutation of CYTOKININ OXIDASE/DEHYDROGENASE (CKX) genes encoding cytokinin-degrading enzymes on these yield-related traits in oilseed rape (Brassica napus L.). We describe the identification of four BnCKX3 and two BnCKX5 genes as regulators of reproductive development in the allotetraploid B. napus. RNA-seq analysis and in situ hybridization showed expression of these genes in reproductive organs. Loss-of-function mutants for each of these CKX gene copies were identified by targeting induced local lesions in genomes (TILLING) and combined by crossing. Sextuple ckx3 ckx5 mutants showed an increased cytokinin concentration and larger and more active inflorescence meristems. They also produced up to 72% more flowers with gynoecia containing 32% more ovules and up to 54% more pods on the main stem. The weight of seeds harvested from the main stem of plants grown in the greenhouse or in the field was increased by 20–32%. Our results show that cytokinin regulates inflorescence meristem and placenta activity in oilseed rape. The work demonstrates the potential to achieve yield enhancement in a dicot crop plant by modulating the cytokinin status through mutagenesis of specific CKX genes.
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Niemann, Michael C. E., Isabel Bartrina, Angel Ashikov, Henriette Weber, Ondřej Novák, Lukáš Spíchal, Miroslav Strnad, et al. "Arabidopsis ROCK1 transports UDP-GlcNAc/UDP-GalNAc and regulates ER protein quality control and cytokinin activity." Proceedings of the National Academy of Sciences 112, no. 1 (December 22, 2014): 291–96. http://dx.doi.org/10.1073/pnas.1419050112.
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The formation of glycoconjugates depends on nucleotide sugars, which serve as donor substrates for glycosyltransferases in the lumen of Golgi vesicles and the endoplasmic reticulum (ER). Import of nucleotide sugars from the cytosol is an important prerequisite for these reactions and is mediated by nucleotide sugar transporters. Here, we report the identification of REPRESSOR OF CYTOKININ DEFICIENCY 1 (ROCK1, At5g65000) as an ER-localized facilitator of UDP-N-acetylglucosamine (UDP-GlcNAc) and UDP-N-acetylgalactosamine (UDP-GalNAc) transport in Arabidopsis thaliana. Mutant alleles of ROCK1 suppress phenotypes inferred by a reduced concentration of the plant hormone cytokinin. This suppression is caused by the loss of activity of cytokinin-degrading enzymes, cytokinin oxidases/dehydrogenases (CKXs). Cytokinin plays an essential role in regulating shoot apical meristem (SAM) activity and shoot architecture. We show that rock1 enhances SAM activity and organ formation rate, demonstrating an important role of ROCK1 in regulating the cytokinin signal in the meristematic cells through modulating activity of CKX proteins. Intriguingly, genetic and molecular analysis indicated that N-glycosylation of CKX1 was not affected by the lack of ROCK1-mediated supply of UDP-GlcNAc. In contrast, we show that CKX1 stability is regulated in a proteasome-dependent manner and that ROCK1 regulates the CKX1 level. The increased unfolded protein response in rock1 plants and suppression of phenotypes caused by the defective brassinosteroid receptor bri1-9 strongly suggest that the ROCK1 activity is an important part of the ER quality control system, which determines the fate of aberrant proteins in the secretory pathway.
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Carrasco, Cristian, Andrés Tittarelli, Natalia Paillaleve, Maeva Del Pozo, Daniel Rojas-Sepúlveda, Omar Barría, Paula Fluxá, et al. "The Evaluation of 17 Gastrointestinal Tumor Markers Reveals Prognosis Value for MUC6, CK17, and CD10 in Gallbladder-Cancer Patients." Diagnostics 11, no. 2 (January 21, 2021): 153. http://dx.doi.org/10.3390/diagnostics11020153.
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Gallbladder cancer (GBC) is an aggressive and highly lethal disease with relatively low global incidence, but one that constitutes a major health problem in Asian and Latin American countries, particularly in Chile. The identification of new tumor-associated markers with potential prognosis value is required for GBC clinical practice. Using immunohistochemistry/tumor tissue microarray, we evaluated the expression of 17 gastrointestinal tumor-associated protein markers (CK7, CK17, CK19, CK20, CKLMW, CKHMW, MUC1, MUC2, MUC5AC, MUC6, CA125, CD10, CEA, vimentin, villin, claudin-4, and CDX2) in primary gallbladder adenocarcinomas from 180 Chilean patients and analyzed potential associations with their pathological and clinical characteristics. Younger female patients with well- to moderately differentiated tumors had a better prognosis than that of older female or male patients with tumors with a similar tumor differentiation grade. Among all analyzed markers, MUC6 expression was associated with better prognosis in patients with well- to moderately differentiated tumors, whereas CK17 or CD10 was associated with worse prognosis in patients with poorly differentiated tumors. In addition, the MUC6+CK17– expression pattern was strongly associated with better prognosis in patients with well- to moderately differentiated tumors, whereas patients with poorly differentiated tumors and with the CK17+CD10+ expression pattern showed worse prognosis. Our results suggest that tumor MUC6, CK17, and CD10 can be considered as potential prognosis markers for GBC.
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Mühlhauser, J., C. Crescimanno, M. Kasper, D. Zaccheo, and M. Castellucci. "Differentiation of human trophoblast populations involves alterations in cytokeratin patterns." Journal of Histochemistry & Cytochemistry 43, no. 6 (June 1995): 579–89. http://dx.doi.org/10.1177/43.6.7539466.
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Cytokeratins (CKs) are related to proliferation and differentiation of epithelial cells. Little knowledge exists about CK patterns in human trophoblast subpopulations (villous and extravillous trophoblasts). To better understand differentiation and function of trophoblast components, we studied the distribution patterns of CKs in the placenta throughout pregnancy. A panel of well-defined monoclonal antibodies against different types of cytokeratins, vimentin, and fibrin, was used on frozen and paraffin sections. CK8, 18, and 19 were expressed in all the villous and extravillous trophoblastic subsets throughout pregnancy. In the first trimester, syncytiotrophoblasts were positive for CK7 and 13 along the basal membrane. As pregnancy progressed there was an increase in intensity of the reaction product and a more diffuse positive staining of CK7 in the cytoplasm of the syncytium, with evident positivity along the apical membrane. CK13 showed similar expression as CK7, but with less intense staining along the apical membrane and less prominent staining in the cytoplasm. Villous cytotrophoblasts were also positive for CK7 and CK13. CK17 was found related to cytotrophoblastic cells in contact with or next to fibrin deposits. Extravillous cytotrophoblasts in cell islands and cell columns were positive for CK13 only in the cell layers located proximal to the villous stroma, whereas the distal and more differentiated cells were negative. CK7 was positive in all epithelial cells of cell islands and columns, but the reaction product was not present in cells deeply migrated into the decidua. Amnion was negative for anti-CK13 antibodies in the first trimester but was positive at term. CK4 and CK16 were not found in the placenta. Our study shows for the first time that the different populations of human placental trophoblast express cytokeratins in developmental, differentiative, and functional specific patterns. These findings can be useful to distinguish and classify the various trophoblastic populations and provide a foundation for studying pathological aspects of the trophoblast.
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Liu, Yang, Xun Wang, Xiaofei Wang, Wensheng Gao, and Chunxiang You. "Identification and Functional Characterization of Apple MdCKX5.2 in Root Development and Abiotic Stress Tolerance." Horticulturae 8, no. 1 (January 10, 2022): 62. http://dx.doi.org/10.3390/horticulturae8010062.
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Cytokinin oxidase/dehydrogenases (CKXs) are the key enzymes in cytokinin degradation and have been widely studied in model plants. Little is known about apple’s (Malus×domestica) CKX genes. Here, using genome-wide analysis, we identified 10 MdCKX genes in apple. The phylogenetics, chromosome locations, and genome structures were then tested. Expression analysis showed that MdCKX genes had different expression profiles in apple, pointing to the different roles. Meanwhile, relative expression analysis showed that these genes have different expression patterns in response to several exogenous cytokinin factors, including trans-zeatin (ZT), thidiazuron (TDZ), and N6-furfuryladenine (KT). Finally, we introduced the MdCKX5.2 gene into Arabidopsis to evaluate its functions, and the results suggested the transgenic Arabidopsis displayed phenotypes related to promoting primary root and lateral root development, response to exogenous ZT, and conferring to drought and salt tolerant. Taken together, our results provide insights on the possible application of the MdCKX5.2 gene for molecular breeding in apples.
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Sanguansin, Sirima, Theerachai Kosanwat, Rachai Juengsomjit, and Sopee Poomsawat. "Diagnostic Value of Cytokeratin 17 during Oral Carcinogenesis: An Immunohistochemical Study." International Journal of Dentistry 2021 (November 22, 2021): 1–9. http://dx.doi.org/10.1155/2021/4089549.
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Background. Little is known about the role of cytokeratin 17 (CK17) during oral carcinogenesis. CK17 expression in oral leukoplakia (OL), the most encountered oral potentially malignant disorders and oral squamous cell carcinoma (OSCC), remains very limited. To determine the role of CK17 during oral carcinogenesis and its potential diagnostic marker in oral premalignant and malignant lesions, this study evaluated CK17 expression in OL without dysplasia, OL with dysplasia, and OSCC. CK17 expression in these tissues was compared with those of normal oral mucosa (NOM). Additionally, the relationship between CK17 expression and clinicopathologic factors of OSCC was investigated. Methods. CK17 expression was evaluated in 186 samples consisting of 12 NOM, 33 OL without dysplasia, 58 OL with dysplasia, and 83 OSCC using immunohistochemistry. The proportion of positively immunostained cells was evaluated and scored. Results. CK17 was expressed in 8.3%, 54.5%, 74.1%, and 90.4% of NOM, OL without dysplasia, OL with dysplasia, and OSCC, respectively. NOM had a significantly lower CK17 score than OL with dysplasia ( p = 0.0003 ) and OSCC ( p < 0.0001 ). A significant association between CK17 expression and histopathologic differentiation of OSCC was found. Tumors with well differentiation had high CK17 expression compared with those of moderate and poor differentiation. Conclusion. CK17 was overexpressed in OL with dysplasia and OSCC, suggesting that CK17 plays a pivotal role in the development of premalignant lesions and OSCC. Of clinical significance, CK17 may be a good diagnostic marker for oral premalignant lesions and OSCC. Additionally, CK17 could be used as an objective tool to classify histopathologic grade in OSCC. The findings that CK17 expression is high in OSCC but low in NOM imply that CK17 may serve as a potential therapeutic target for OSCC.
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Liu, Haiyan, Jianhui Shi, Vasuki Anandan, Hanlin L. Wang, David Diehl, Joseph Blansfield, Glenn Gerhard, and Fan Lin. "Reevaluation and Identification of the Best Immunohistochemical Panel (pVHL, Maspin, S100P, IMP-3) for Ductal Adenocarcinoma of the Pancreas." Archives of Pathology & Laboratory Medicine 136, no. 6 (June 1, 2012): 601–9. http://dx.doi.org/10.5858/arpa.2011-0326-oa.
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Context.—Differentiation of ductal adenocarcinoma of the pancreas from nonneoplastic pancreatic tissues can be challenging, especially in small biopsy and fine-needle aspiration specimens. Objective.—To investigate the utility of 26 immunohistochemical markers (CAM 5.2, CK [cytokeratin] 7, CK20, CK17, CK19, MUC1, MUC2, MUC4, MUC5AC, MUC6, p53, DPC4/SMAD4, CDX2, pVHL [von Hippel-Lindau tumor suppressor gene protein], S100P, IMP-3 [insulin-like growth factor 2 messenger RNA binding protein 3], maspin, mesothelin, claudin 4, claudin 18, annexin A8, fascin, PSCA [prostate stem cell antigen], MOC31, CEA [carcinoembryonic antigen], and CA19-9 [cancer antigen 19-9]) in the diagnosis of ductal adenocarcinoma of the pancreas. Design.—Immunohistochemical staining for these markers was performed in 60 cases of pancreatic ductal adenocarcinoma on routine and tissue microarray sections. In addition, immunohistochemical staining for maspin, S100P, IMP-3, and pVHL was performed on cell blocks from 67 pancreatic fine-needle aspiration cases, including 44 cases of pancreatic ductal adenocarcinoma. Results.—The results demonstrated that (1) more than 90% of cases of ductal adenocarcinoma were positive for maspin, S100P, and IMP-3; (2) nearly all adenocarcinoma cases were negative for pVHL, whereas nonneoplastic ducts and acini were positive for pVHL in all cases; (3) normal/reactive pancreatic ducts were frequently positive for CK7, CK19, MUC1, MUC6, CA19-9, MOC31, PSCA, mesothelin, annexin A8, claudin 4, and claudin 18; (4) normal pancreatic ducts were usually negative for IMP-3, maspin, S100P, CK17, MUC2, MUC4, and MUC5AC; (5) 60% of adenocarcinomas were negative for DPC4/SMAD4; and (6) strong background staining was frequently seen with fascin, PSCA, and annexin A8. Conclusions.—pVHL, maspin, S100P, and IMP-3 constitute the best diagnostic panel of immunomarkers for confirming the diagnosis of pancreatic ductal adenocarcinoma in both surgical and fine-needle aspiration specimens.
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Hong, Soon Auck, Su Hyun Yoo, Jene Choi, Stanley J. Robboy, and Kyu-Rae Kim. "A Review and Update on Papillary Immature Metaplasia of the Uterine Cervix: A Distinct Subset of Low-Grade Squamous Intraepithelial Lesion, Proposing a Possible Cell of Origin." Archives of Pathology & Laboratory Medicine 142, no. 8 (April 13, 2018): 973–81. http://dx.doi.org/10.5858/arpa.2017-0267-oa.
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Context.— Papillary immature metaplasia (PIM) is a known papillary cervical lesion associated with low-risk human papillomavirus (LR-HPV). Objective.— To evaluate additional clinicopathologic features and the HPV genotypes of PIM and discuss the presumptive cell of origin. Design.— A total of 26 PIM cases were evaluated by p16INK4a, cytokeratin (CK) 7, and CK17 immunohistochemical stainings. Human papillomavirus genotyping was performed, by using HPV DNA Chip, HPV polymerase chain reaction (PCR), and real-time PCR. Results.— Histologically, PIM forms either a papillary mass (n = 21 of 26, 81%) or a slightly elevated/flat plaque (n = 5, 19%). All cases contain variable amounts of mucinous epithelia within the lesions. Koilocytosis was identified in 15 of the 26 cases (58%). Sixteen cases (61%) were associated with LR-HPV (types 6, 11, or 42), but 3 cases (12%) with high-risk (HR) HPV (16, 16/18, and 33), 2 cases (8%) with mixed LR- and HR-HPV (6/16 and 11/58), while 2 cases (8%) were negative, but p16INK4a immunostaining showed nonblock positivity in all cases. Eight (31%) had high-grade squamous intraepithelial lesion (HSIL) in the adjacent mucosa, 4 (50%) of which showed direct continuity. Identical HPV subtypes were confirmed in separately microdissected cases from PIM and adjacent HSIL. Most lesions (n = 24, 92%) expressed CK17 (reserve cell marker) in a bottom-heavy pattern and CK7 (squamocolumnar junction [SCJ] marker) in a top-heavy pattern, while most cases of low-grade squamous intraepithelial lesion (LSIL) were negative for both markers. Conclusions.— Our results suggest that PIM is a distinct subset of LSIL showing a productive HPV infection, but PIM involves the transformation zone and is proximal to SCJ, while LSIL is mostly from ectocervix or distal to the SCJ.
Dissertations / Theses on the topic "CKX7"
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Köllmer, Ireen [Verfasser]. "Funktionelle Charakterisierung von CKX7 und cytokininregulierten Transkriptionsfaktorgenen in Arabidopsis thaliana / Ireen Köllmer." Berlin : Freie Universität Berlin, 2009. http://d-nb.info/1027498353/34.
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DI, MARZO MAURIZIO. "THE MADS-DOMAIN SEEDSTICK PLAYS FUNDAMENTAL ROLES DURING TRANSMITTING TRACT DEVELOPMENT AND FRUIT GROWTH IN ARABIDOPSIS THALIANA." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/717626.
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The MADS-box gene SEEDSTICK (STK) controls several aspects of plant reproduction. STK is co-expressed with CESTA (CES), a basic Helix-Loop-Helix (bHLH) transcription factor-encoding gene. CES was reported to control redundantly with the brassinosteroid positive signaling factors BRASSINOSTEROID ENHANCED EXPRESSION1 (BEE1) and BEE3 the transmitting tract development. Through the characterization of the stk ces-2 double mutant, we observed that STK and CES act together in the regulation of transmitting tract development. Combining the stk with ces-2 bee1 bee3 we have obtained the quadruple mutant showed a clear increase of the unfertilized ovules and septum defects. In the quadruple mutant carpel fusion was compromised, causing the formation of holes at the center of the septum where transmitting tract differentiates. These phenotypes do not allow a proper pollen tube growth in the double mutant stk ces-2, and even more in the quadruple mutant bee1 bee3 stk ces-2 leading to high number of unfertilized ovules. The transcriptome profile of the quadruple mutant bee1 bee3 stk ces-2 compared to wild type revealed a small subset of misregulated genes, which probably act downstream of the transcription factor described above, mainly involved in cell death, the extracellular matrix of the transmitting tract, cell wall composition and auxin signaling. This specific subset of downstream target genes controlled directly or indirectly by STK-CES-BEE1-BEE3 protein complex, open doors to a new regulatory network controls transmitting tract development. Altogether our data reveal new insights in the regulation of transmitting tract development together by bHLH and MADS-box transcription factors.Upon fertilization, the ovary increases in size and undergoes a complex developmental process to become a fruit. The fruit of Arabidopsis thaliana is named silique. We show that cytokinins (CK), required to define ovary size before fertilization, have to be degraded to obtain the correct fruit growth. The expression of CYTOKININ OXIDASE DEHYDROGENASE 7 (CKX7), which encodes a cytosolic CK degrading enzyme, is directly regulated post-fertilization by the MADS-box transcription factor STK. Similar to stk, two ckx7 mutant alleles possess shorter fruits compared to wild type. Quantification of CKs revealed that stk has high CK levels during fruit elongation, which negatively control cell expansion during fruit development, compromising fruit growth. Overexpression of CKX7 partially complements the stk fruit phenotype. We show that CKX6 does not regulate fruit elongation process. CKX6 encodes for one of the fourth CK degrading enzymes that acts in the apoplast. The CKX6 promoter is not active during fruit elongation phases. Moreover, the ckx6 mutant does not display differences in fruit length when compared to wild type. Finally, we show that STK is also required for the correct expression of the MADS-box gene FUL, which is considered the master regulator of valve elongation in fruit. The double mutant stk ful displayed shorter siliques when compared to wild type, but also respect to the two single mutants. The additive phenotype of the double mutant stk ful suggests the possibility that the two MADS-box transcription factors act in two parallels pathways that can regulate fruit elongation process. Overall, we provide novel insights into the regulatory pathway that control fruit growth.
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Mameaux, Sabine Hee. "Characterisation of the cytokinin oxidase/dehydrogenase 2 (CKX2) orthologues in bread wheat." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609648.
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Guo, Tianqi [Verfasser]. "Functional characterization of clade-I HIPP proteins from Arabidopsis thaliana interacting with cytokinin-catabolizing CKX enzymes / Tianqi Guo." Berlin : Freie Universität Berlin, 2020. http://d-nb.info/1206934549/34.
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Fiala, Friederike Anna Verfasser], Margitta [Akademischer Betreuer] [Gutachter] Retz, and Jürgen E. [Gutachter] [Gschwend. "Nachweis von disseminierten Tumorzellen im Knochenmark von Patienten mit Harnblasenkarzinom mittels quantitativer real-time PCR und der molekularbiologischen Marker CK20, CK14, CK17, UPK2, SPINK1 und TMPRSS2 / Friederike Anna Fiala ; Gutachter: Jürgen E. Gschwend, Margitta Retz ; Betreuer: Margitta Retz." München : Universitätsbibliothek der TU München, 2016. http://d-nb.info/1114885061/34.
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Anderson-Long, Maria Alana. "Responses to Normative Disruption of the Gender Binary Through the Creation of Gender Inclusive Housing." Thesis, 2019. https://doi.org/10.7916/d8-ckx0-yh15.
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This study, a multisite qualitative case study, examines the responses of three institutions of higher education to normative disruption of the gender binary. Normative disruption, or the challenging of the social status quo, occurs when power structures in society are pushed back against. Central to this study is the use of open systems theory, which positions higher education as a subsystem of American society, and therefore responsive to changes in the environment external of the institution. This study investigates how, if at all, these case sites employed Gender Inclusive Housing (GIH) policies as an institutional response to changes in how gender was conceptualized on their campus. Specifically, this study addresses: 1. how changes in societal norms around the gender binary influence colleges and universities, 2. in what ways institutions respond to such changes, 3. what ways institutions reestablish organizational homeostasis around an expanded concept of gender, and 4. how institutional characteristics influence decision-making responses.
Out of the findings of this study emerged the Model of Normative Disruption, a mechanism that can be utilized to understand institutional decision-making responses to normative disruption. The findings of this study suggest: 1. various societal and institutional factors influence the ways in which normative disruption manifests at a college or university, 2. institutional characteristics and culture impact all responses to normative disruption, and can either support or hinder change, 3. GIH is one mechanism of responding to normative disruption, but, depending on institutional characteristics, may not be sufficient enough change to reestablish organizational homeostasis, and 4. if the institutional culture is not an amenable environment to such changes, organizational homeostasis is difficult to reestablish.
This study concludes with implications for theory, research, and practice. Importantly, I suggest that GIH policies might serve as an opportunity for administrators to begin the necessary conversations of understanding the myriad cisgenderist policies, practices, and culture that exist within systems of higher education.
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Vícha, Daniel. "Ovlivnění odpovědi rostlin na teplotní stres modulovanými hladinami cytokininů - fenomická a protemická analýza." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-250419.
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Cytokinins are important group of phytohormones regulating many physiological processes ranging from cell division to programmed cell death. This thesis is focused on effects of cytokinin levels in response to heat stress in Arabidopsis thaliana. Analysis of transgenic plants with regulated expression of ipt and HvCKX showed that cytokinins and their optimal levels play important role in the morphological alterations induced by heat stress. Seedlings with increased and decreased levels of cytokinins exhibit inhibition of petioles growth, decreased length of blades of true leaves and reduced leaf area. To obtain insights into molecular events underlying early response to heat stress LC-MS analysis of whole proteom was performed. Analysis revealed 57 differentialy regulated proteins in response to heat stress in Columbia ecotype. On the cellular level, most of the proteins were located in cytosol (47 %) nebo plastids (32 %). Coparative analysis between wild-type seedlings and seedlings with decreased level of cytokinins confirmed 31 proteinInfluencing plant responses to temperature stress modulating cytokinin levels - fenomic and proteomic analysiss regulated by cytokinins in response to heat stress. Among these proteins, desarurase 7 and Tudor SN1 protein were previously found as important factors in response to heat stress.
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黃茂栓. "Expression of Pan CK, CK4, CK5, CK7, CK8 & Ki-67 in reduced enamel epithelium." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/70339116856511332100.
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碩士臺北醫學大學
口腔復健醫學研究所
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Background and purposes: Dentigerous cysts occur most frequently in the impacted third molar areas of the jawbone. The lining epithelium of the dentigerous cyst is originated from the reduced enamel epithelium. The purposes of this study were to examine the histological features of dental follicles around the impacted molars, to investigate the characteristic features of reduced enamel epithelial cells, and to assess the relationship between the reduced enamel epithelium and the pathogenesis of the dentigerous cyst. Materials and methods: The study group included 57 specimens of dental follicular tissues excised from tissues around the impacted molars. The tissue sections were prepared by routine histological procedures. The histological features of dental follicles were studied by examining the hematoxylin & eosin-stained slides. In addition, immunohistochemical staining with anti-pan CK, -CK 4, -CK 5, -CK 7, -CK 8 and -Ki-67 antibodies was used to investigate the characteristic features of reduced enamel epithelial cells. PAS stain was used to observe whether there was the presence of glycogen or neutral mucopolysaccharide in the reduced enamel epithelial cells. Results: Most of the dental follicular tissues had reduced enamel epithelium of stratified squamous type on their inner surface. The dental follicular tissues were occasionally lined by a single layer of high columnar epithelium on their inner surface. Edema, hemorrhage, congestion and a chronic inflammatory cell infiltrate were often found in the connective tissue of dental follicles. By immunohistochemistry, we found that the reduced enamel epithelial cells were strongly positive for pan CK, weakly positive for CK 5, and negative for CK 4, CK 7, CK 8 and KI-67. By PAS stain, we also discovered that the reduced enamel epithelial cells did not contain glycogen or neutral mucopolysaccharide. Conclusions: We conclude that the reduced enamel epithelial cells do not have characteristic features of non-keratinized stratified squamous epithelium (CK 4-negative), of simple columnar epithelium (CK 7- and CK 8-negative), and of cells with proliferation ability (Ki-67-negative). Furthermore, they are not metabolically active cells (glycogen-negative). There is no direct evidence to support an intimate relationship between the reduced enamel epithelium and the pathogenesis of the dentigerous cyst. A chronic inflammatory cell infiltrate and edema are frequently observed in the connective tissue of the dental follicles. However, further studies are needed to evaluate whether there are evidences to support a direct association between the chronic inflammation and the pathogenesis of the dentigerous cyst. Keywords: Reduced enamel epithelium, Cytokeratin, immunohistochemistry, dental follicles.
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Reusche, Michael. "Analyse der Verticillium longisporum induzierten Seneszenz und Transdifferenzierung in Arabidopsis thaliana." Doctoral thesis, 2011. http://hdl.handle.net/11858/00-1735-0000-0006-AE32-1.
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Cunha, Marta Raquel Fortuna da. "Expressão das citoqueratinas 7 e 20 em adenocarcinomas do endométrio de gata." Master's thesis, 2016. http://hdl.handle.net/10348/5434.
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Dissertação de Mestrado Integrado em Medicina Veterinária, Ciências VeterináriasEm Patologia Veterinária, os adenocarcinomas do endométrio de gata (AEGs) são considerados raros. Estudou-se a expressão das Citoqueratinas 7 (CK7) e 20 (CK20) em 25 úteros normais de gata, tanto na fase folicular (n=15), como secretora (n=10) do ciclo éstrico, bem como em 49 amostras de AEG. As amostras foram submetidas à técnica de imunohistoquímica indireta, com os anticorpos anti-CK7 e anti-CK20. A intensidade da marcação foi avaliada em Fraca, Moderada ou Forte. Foram ainda avaliadas a percentagem e localização da marcação. Nos úteros normais, a fase folicular apresentava uma maior expressão da CK7, comparada com a fase secretora. A expressão da CK20 mostrava-se moderada em ambas as fases. Comparando tecidos normais com neoplasias, é possível notar uma diminuição na expressão da CK7, enquanto a expressão da CK20 aumenta nas amostras tumorais. Este é um estudo inovador nesta espécie animal, que nos permitiu adquirir conhecimentos nesta área e melhorar a caracterização dos AEGs, sendo uma mais valia para o seu correto diagnóstico, podendo no futuro ser um válido contributo para o conhecimento da sua progressão clínica e prognóstico.
In Veterinary Pathology, endometrial adenocarcinomas are considered rare in the queen. We studied the expression of Cytokeratins 7 (CK7) and 20 (CK20) in 25 normal queen uteri, both in the follicular (n=15) and secretory (n=10) phases of the oestrous cycle, as well as in 49 samples of endometrial adenocarcinoma. The samples were analysed through indirect immunohistochemistry, with the antibodies anti-CK7 and anti-CK20. The intensity of the staining was evaluated as Weak, Moderate or Strong. We also assessed the percentage of expression and its location. In normal uteri, the follicular phase presented a greater CK7 expression, compared to the secretory phase. The expression of CK20 was moderate in both phases. When comparing normal with neoplastic tissues, it is possible to perceive a decrease in the CK7 expression, while the expression of CK20 increases in the neoplastic samples. This is an innovative study in this species, which has allowed us to acquire knowledge in the field of the endometrial adenocarcinomas of the queen, also being a useful resource for its correct diagnosis. It may, in the future, be an effective contribution to the understanding of their clinical progression and prognosis.
Book chapters on the topic "CKX7"
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A. Heckman, Carol. "Cytokeratins of Tumorigenic and Highly Malignant Respiratory Tract Epithelial Cells." In Keratinocyte Biology: structure and function in the epidermis [Working Title]. IntechOpen, 2022. http://dx.doi.org/10.5772/intechopen.102592.
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In malignant airway epithelial cells, structural abnormalities were evident from the cytokeratin organization. To determine whether the cytokeratins themselves were responsible, an in vitro model for bronchogenic carcinoma, consisting of three highly malignant lines and three less tumorigenic lines, was studied. Cytokeratins were evaluated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). When typical constraints on tumors were relieved by in vitro culture, lines showed profiles resembling normal, primary cells. The CK5/CK14 combination, characteristic of basal epithelial layers, was represented by CK6A/CK14. CK17 was invariably present, while CK5, CK7, CK8, CK19, and CK42 content varied. CK19 appeared to substitute for the rarely observed CK18. While lacking the common CK8/CK18 combination of hyperproliferative cells, an invasive, metastasizing line had CK6A/CK7 or CK8 with CK19 suggesting derivation similar to adenocarcinomas. Bands of CK19 and actin migrated to higher pI in tumorigenic and malignant lines than in normal cells. Ubiquitinated acidic cytokeratins with a low isoelectric point (pI) and high molecular weight (MW) showed no consistent differences in lines that differed in growth potential. Type II made up 49–52% of total cytokeratins in nonmalignant lines, whereas highly malignant lines showed lower levels. Posttranslational modifications were identified but could not explain the shortfall of basic cytokeratins.
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Trifunović-Momčilov, Milana, and Václav Motyka. "Transgenic AtCKX Centaury Plants Grown In Vitro." In Plant Breeding - Current and Future Views [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.94836.
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The production and breeding of plants with desired properties are possible by a fundamental biotechnological technique, genetic engineering. Applying and developing of genetic engineering procedures also enable preservation and improvement of plant species endangered in nature, including medicinal plant common centaury (Centaurium erythraea Rafn.). Numerous developmental processes in plants are controlled by cytokinins (CKs). The only so far known enzyme involved in CK catabolism is cytokinin oxidase/dehydrogenase (CKX). Genes coding for two Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were successfully introduced into centaury root explants. Subsequently, the contents of endogenous CKs in AtCKX-overexpressing centaury plants grown in vitro were investigated. Simultaneous secondary metabolite analyses showed antibacterial and antifungal activity of transgenic centaury plants and suggested their use as potential producers of anti-cancer compounds. Considering that centaury can inhabit saline soils in natural habitats, following investigations included evaluation of salinity tolerance in vitro. All obtained and summarized results indicated that transgenic AtCKX centaury plants could serve as a suitable model for studies of numerous physiological and developmental processes under endogenous phytohormonal control.
Conference papers on the topic "CKX7"
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Menghesha, H., F. Doerr, G. Schlachtenberger, A. Camo, A. Amorin Estremadoyro, M. Heldwein, T. Wahlers, and K. Hekmat. "CK7 beeinflusst das Langzeitüberleben bei Lungenkrebspatienten." In 31. Jahrestagung der Deutschen Gesellschaft für Thoraxchirurgie. Georg Thieme Verlag, 2022. http://dx.doi.org/10.1055/s-0042-1754194.
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Yang, Haisu, Rosalba Tamayo, Bruce Horten, Moacyr DaSilva, Maryann Gangi, Evelyn Vazquez, Daisy Joseph, Patricia Okamoto, and Thomas Scholl. "Abstract LB-274: Clinical significance of MUC1, MUC2 and CK17 expression patterns in the diagnosis of pancreatic carcinoma." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-lb-274.
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Qin, Mei-Hua, Hong-Dong Guo, Mei-Juan Qin, and Gang-Ping Wang. "Applications of Immunohistochemistry: E-cadherin, CK7, CK20, CD38, CD56 and Ki-67 in Distinguishing Plasmacytoid Urothelial Carcinoma from Small Cell Neuroendocrine Carcinoma." In 2015 International Conference on Medicine and Biopharmaceutical. WORLD SCIENTIFIC, 2016. http://dx.doi.org/10.1142/9789814719810_0041.
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Soni, Abhishek, Nupur Bansal, A. K. Dhull, Vivek Kaushal, Rajeev Atri, and Monica Verma. "Diagnostic dilemma of mesonephric adenocarcinoma cervix." In 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685283.
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Introduction: Mesonephric carcinoma is a rare type of epithelial tumor of the uterine cervix which derive from the remnants of the paired mesonephric (Wolff’s) ducts. The incidence of such neoplasms is difficult to determine due to rarity, previous misclassification of clear cell carcinomas and yolk sac tumours as mesonephric carcinomas and potential underreporting due to misclassification of mesonephric carcinoma as Mullerian tumours or mesonephric hyperplasia. The evidence regarding the clinical course, prognosis and optimal treatment is limited. Materials and Methods: Searches were performed on MEDLINE, EMBASE and Google Scholarly articles. All the relevant articles were included in the study. Only approximate 40 cases have been reported till now. Discussion: Mesonephric adenocarcinoma cervix has different morphologies like ductal, tubular, solid, retiform, sex-cord like pattern, clear cell and serous papillary structures. IHC assessment is helpful in differentiating it from Mullerian counterpart, as it is negative for CEA, CK20, p16, PAX2, ER/PR and vimentin and positive for CD10, calretinin, CK7, CAM5.2 and EMA. It has no relation with HPV infection. Unlike squamous epithelial carcinoma, it is rarely presenting with the abnormal cervical smear result, has more advanced age at presentation and its incidence does not appear to decline with age. The diagnosis has been supported by endometrial curettings, directed/cone cervical biopsies and hysterectomy specimens. The majority of patients are diagnosed at stage IB with mean DFS of 48.6 months. Recurrence rate is 23%, with a mean interval of 40 months. Hysterectomy is the primary treatment. Advanced stage disease of adenocarcinoma seemed to respond to radiotherapy, but for the MMMTs the combination of chemotherapy with radiotherapy appears to be preferable. Conclusion: Rarity of the neoplasm, varied morphology, mix presentation and very low number of cases leads to difficulty in correct diagnosis in a small biopsy specimen. IHC helpful in differentiating it from other lesions.
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Yela, Daniela Angerame, Mariana Sousa Sguerra Silva, and Cristina Laguna Benetti-Pinto. "Correlação entre os aspectos anatamopatológicos com a dor pélvica de mulheres com endometriose profunda." In 45º Congresso da SGORJ XXIV Trocando Ideias. Zeppelini Editorial e Comunicação, 2021. http://dx.doi.org/10.5327/jbg-0368-1416-20211311028.
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Introdução: O tratamento da endometriose pode ser clínico, cirúrgico ou combinado. Uma das principais indicações cirúrgicas no tratamento da endometriose profunda é a má resposta ao tratamento clínico. Acredita-se que as mulheres que têm focos com maior população glandular em relação à estromal e/ou fibrótica possam ter melhor resposta à hormonioterapia quanto à dor, bem como aquelas cujos focos de endometriose apresentem maior número de receptores de estrógeno/progesterona. Objetivo: correlacionar os aspectos morfológicos com a dor pélvica das mulheres com endometriose profunda. Métodos: Foi realizado estudo retrospectivo com 45 mulheres com endometriose profunda que foram submetidas ao tratamento cirúrgico num hospital terciário de 2007 até 2017. Os dados analisados foram idade, paridade, índice de massa corpórea, local de acometimento da doença, tratamento hormonal antes da cirurgia, sintomatologia álgica e análise morfométrica. As lâminas histológicas das peças cirúrgicas foram revistas e, com o uso de um software para estudo morfométrico (ImageJ®), as porcentagens de tecidos estromal/ glandular foram calculadas nos cortes histológicos. Resultados: A média de idade das mulheres foi 38,1±7,2 anos. O nível de dor médio foi 9,07±1,56 e o tempo médio do início dos sintomas foi de 4,22±2,1 anos. Entre as 45 mulheres, 60% eram nuligestas e 96% realizaram tratamento hormonal prévio à cirurgia. A média de expressão dos marcadores anatomopatológicos CD10, CK7 e S100 foi de 16,22±10,9%, 9,59±6,2% e 7,06±5,1%, respectivamente. Não houve diferença significativa entre a expressão dos marcadores e o local de acometimento da endometriose e a realização de tratamento. Não se observou correlação entre a expressão dos marcadores e a idade, o nível de dor e o tempo dos sintomas. Conclusão: Mulheres com tratamento hormonal não apresentam diferença na composição histológica do tecido endometrial e não há associação entre os aspectos morfométricos das lesões de endometriose e a dor.
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Bastos, Alberto Freaza Lobão, Paula de de Holanda, José Augusto Machado, Nereu Guerra Neto, Amanda Mendes Soares, and Yara Lúcia Mendes Furtado de Melo. "O uso da imuno-histoquímica no diagnóstico diferencial do adenocarcinoma endometrial: relato de caso." In 44° Congresso da SGORJ - XXIII Trocando Ideias. Zeppelini Editorial e Comunicação, 2020. http://dx.doi.org/10.5327/jbg-0368-1416-2020130202.
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Introdução: O câncer de corpo uterino é um dos tumores ginecológicos mais prevalentes na pós-menopausa, sendo o adenocarcinoma o mais frequente. Em alguns casos, o sítio primário, endométrio ou colo uterino, só pode ser definido pela imuno-histoquímica. Objetivo: Relatar caso de adenocarcinoma de endométrio de conclusão diagnóstica com estudo imuno-histoquímico. Relato de Caso: Paciente de 48 anos, G IV P III, índice de massa corpórea (IMC) 21 kg/m2, vem encaminhada pela Clínica da Família em razão de pólipo em colo uterino. Referia sangramento transvaginal contínuo e discreto, dispareunia, corrimento abundante e dor hipogástrica. Trouxe resultado de citologia (normal) e ultrassonografia transvaginal (USG TV) com útero aumentado de volume, eco endometrial 19 mm, imagem nodular hipoecoica no canal cervical. Ao exame: colo fibroelástico, doloroso à mobilização, dor à palpação de anexos, útero aumentado de volume. Foi indicado tratamento de doença inflamatória pélvica (DIP) e retorno para avaliar histeroscopia. Retornou em dois meses, com mesma sintomatologia e tratamento inadequado para DIP (apenas doxiciclina). Indicou-se internação hospitalar para tratamento. Mantinha dor pélvica e saída de secreção purulenta em grande quantidade do colo uterino. Foi indicada curetagem uterina pela impossibilidade de ser submetida à vídeo-histeroscopia. Retornou no mês seguinte após curetagem, com queixa de sinusorragia. Ao exame: útero de volume aumentado, colo uterino endurecido, volumoso, com infiltração tumoral. Toque retal: paramétrio infiltrado em terço médio à esquerda e proximal ao colo à direita. Resultado histopatológico da curetagem: adenocarcinoma moderadamente diferenciado, não se podendo definir se a origem é de colo ou endométrio. Optou-se por imuno-histoquímica para definição diagnóstica. Resultado: CK7, receptores de estrogênio positivos e vimentina, antígeno carcinoembriônico (CEA) e CK20 negativos; favorecem diagnóstico de adenocarcinoma do endométrio do tipo endometrioide grau 2. Conclusão: O diagnóstico do adenocarcinoma de endométrio, geralmente, é histológico, porém o uso da imuno-histoquímica é importante principalmente em casos de envolvimento do colo uterino.
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Carneiro Filho, Genilson Gomes, Flávia Maria de Souza Climaco, Afranio Coelho-Oliveira, Bianca Kurtz Fontoura, Maria Celia Resende Djahjah, and Ana Helena Correia Carneiro. "Carcinoma primário em mama acessória axilar: relato de caso." In 44° Congresso da SGORJ - XXIII Trocando Ideias. Zeppelini Editorial e Comunicação, 2020. http://dx.doi.org/10.5327/jbg-0368-1416-2020130231.
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Introdução: A incidência de polimastia na população varia de 0,4 a 6,0%. O tecido glandular ectópico pode ser acometido por patologias benignas ou malignas. Entretanto, o carcinoma das mamas acessórias é raro, representando cerca de 0,3 a 0,6% de todos os cânceres de mama. Objetivo: Relatar um caso de carcinoma em mama acessória axilar, discutindo as características clínico-patológicas, as dificuldades do diagnóstico e as controvérsias em relação ao tratamento, em razão de sua raridade. Relato de Caso: Paciente de 61 anos, branca, apresenta queixa de nódulo em região axilar há um mês, com dor à palpação. Refere hipertensão arterial e hipotireoidismo. Menarca aos 12 anos. IIIG IIP IA. Amamentou por dez meses. Menopausa aos 52 anos. Nega reposição hormonal. Nega história familiar de neoplasias. Ao exame, apresenta mamas simétricas, flácidas e indolores. Ausência de retrações, abaulamentos ou nodulações. Presença de tecido glandular ectópico em região axilar direita, com nódulo de consistência endurecida, não aderido a planos profundos, medindo 2,0×3,0 cm, com lesão ulcerada não exsudativa. A mamografia demonstrou padrão de lipossubstituição, sem sinal de doença. BI-RADS I. A ultrassonografia detectou lesão nodular de alta densidade com limites imprecisos, espiculada, em mama acessória na região axilar direita. Mamas tópicas sem alterações. BI-RADS V. Realizada core biópsia com laudo de adenocarcinoma pouco diferenciado com padrão de crescimento difuso ou em fileiras, podendo corresponder a carcinoma lobular infiltrante ou a ductal infiltante ou ductal infiltrante com características lobulares. Imuno-histoquímica: CK7 positivo, CK20 negativo, antígeno carcinoembriônico positivo, RE positivo (90%), RP positivo (80%), KI67 positivo (50%), TTF-1 negativo, CERB B-2 negativo, E-CADERINA negativa. Indicada ressecção de mama acessória axilar e biópsia de linfonodo sentinela, sendo realizada injeção peritumoral de azul patente. O laudo histopatológico demonstrou carcinoma ductal infiltrante pouco diferenciado grau 3, com características lobulares, margens livres de neoplasia. Presença de metástase em um de dois linfonodos examinados. Realizada radioterapia adjuvante, quimioterapia e uso de anastrozol. Paciente permanece em acompanhamento, sem evidência de doença. Discussão: O carcinoma em mama acessória é raro e em 80% dos casos localiza-se na região axilar. O tipo histológico mais frequente é o carcinoma invasivo do tipo não especial (72%), seguido do lobular infiltrante. O diagnóstico, muitas vezes, é tardio, já que a mamografia pode não abordar adequadamente o tecido glandular. Incidências adicionais como a oblíqua e a craniocaudal forçada, assim como a ultrassonografia, permitem maior visualização do tecido ectópico. A ressecção da mama acessória com margens livres é o tratamento cirúrgico indicado. Não é necessária mastectomia, uma vez que a mama homolateral não está comprometida. A biópsia do linfonodo sentinela é uma alternativa viável na ausência de comprometimento ganglionar.
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Tiwari, Alok, Dhananjay Gughe, Radhika Dureja, and Satinder Kaur. "Synchronous primary malignancy of ovary and cervix with different histopathology: A rare case report." In 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685388.
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Concurrent different histopathological types of gynecologic tumors arise rarely. We present ovarian serous and cervical squamous cell carcinoma formed synchronously. A 51-year-old woman with a poor general condition was admitted with gradual distension of abdomen for 1 year with gradual loss of weight and appetite for the last three months and pain in the abdomen and irregular vaginal bleeding for the last two months. There was no family history of malignancy of genital tract, breast or colon. On examination she was cachexic, pale, dehydrated, tachypnoeic and had edema over feet. Per abdomen examination revealed solid, non-mobile palpable mass arising from pelvis. Per vaginal examination revealed large mass in pelvis and uterus can not be felt separately on per speculum examination there was small endocervical erosion, hypertrophied cervix. On per rectal examination bilateral parametria were free. Her tumor marker were evaluated and CA-125 was found to be raised (CA 125: 915.6 u/ml U/mL); rest tumor markers were normal. Cervical punch biopsy was suggestive of moderately differentiated carcinoma and pap smear was also suggestive of cervical cancer. MRI findings revealed a mass of altered signal intensity 2.5 × 1.5 × 2.2 cm with diffusion restriction and post contrast enhancement in the anterior lip of cervix and another large, lobulated predominantly solid mass, hypo intense on T1, intermediate on T2 with diffusion restriction and post contrast enhancement in the right adnexal region abutting the small bowel and sigmoid colon optimal debulking surgery with standard protocol was done. Histopathology report revealed squamous cell carcinoma of cervix, grade III and high grade serous cystadenocarcinoma of ovary. Tumour deposits from ovary were seen on right fallopian tube and right parametrium. Squamous cell carcinoma cervix involved ectocervix, endocervix and infiltrated near full thickness of cervical stroma, endomyometrium, vaginal cuff, paracervical tissue omentum and appendix were free of tumour. Twenty five right pelvic lymphnodes dissected were free of tumour, (00/25). One out of fifteen lymphnode dissected were involved with extra capsular extent, 01/15 and thirteen para aortic lymph node dissected were free of tumor. Immunohistochemistry markers: Ovarian mass-tumour cell expressed ck, vimentin, wt-1 with focal Ck positivity, no expression of ck20, p63, ck5/6 and CEA seen. Cervical tumour-tumour cells expressed ck, ck7, p63 and ck5/6 no expression of ck20, wt-1. Based on our case report we need to keep in mind that even if patient presents with symptoms pertaining to a single malignancy; still the rare possibility of synchronous malignancies should be looked for by doing proper investigations. In our case, patient had symptoms pertaining to ovarian malignancy; whereas cervical malignancy was diagnosed after investigating the patient. Histologic examination should be done properly as the prognosis depends on the malignancies being metastatic or synchronous one appropriate management should be offered in all such cases. Long term follow up of such patients should be maintained to determine the prognosis.
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Bohm, Emanuelle Fick, and Ernesto de Paula Guedes Neto. "Metástase de carcinoma de sítio primário de mama para o trato genital feminino: relato de caso e revisão de literatura." In 44° Congresso da SGORJ - XXIII Trocando Ideias. Zeppelini Editorial e Comunicação, 2020. http://dx.doi.org/10.5327/jbg-0368-1416-2020130245.
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Introdução: Os sítios mais comuns de metástases de câncer de mama são pulmões, ossos, fígado e cérebro. Sítios menos comuns incluem trato gastrointestinal, pâncreas, baço, tireoide, adrenais, rins, coração e trato genital feminino (TGF). As metástases no TGF de sítios primários distantes são incomuns, mas quando se apresentam, tendem a estar envolvidas com os ovários ou o endométrio. O relato deste estudo retrata a ocorrência de neoplasia maligna metastática paratubária em paciente com carcinoma primário de mama. Objetivos: Relatar o caso de uma paciente com metástase de carcinoma de sítio primário de mama para o TGF e revisar na literatura disponível tal ocorrência. Material e Métodos: As informações do caso foram obtidas a partir da revisão de prontuário, registro fotográfico diagnóstico da paciente e revisão de literatura. A revisão foi realizada na base de dados do PubMed, em estudos publicados nos últimos 20 anos (2000‒2020), com os termos “breast cancer” AND “metastasis” AND “female genital tract”. Os artigos selecionados foram estudos meta-analíticos, casos clínicos e revisões na língua inglesa. Resultados e Conclusão: Paciente, sexo feminino, 70 anos, G1P1. Há 20 anos submetida à mastectomia esquerda apresentando carcinoma ductal invasor (CDI), com RE e RP positivo e Human Epidermal growth factor Receptor-type 2 (HER2) negativo. Realizou histerectomia com anexotomia profilática com diagnóstico de neoplasia maligna metastática em tuba esquerda com 1:1 cm no maior eixo. Exame imuno-histoquímico apresentando GATA3 negativo, CK7, Mamoglobina, PAX8, RP e RE positivos. Exames laboratoriais e de imagem pré-operatórios negativos para neoplasia. O CDI é o tipo mais comum de câncer de mama invasivo, seguido pelo carcinoma lobular invasivo (CLI). Contudo, metástases para o TGF são mais frequentemente associadas ao CLI do que ao CDI. Estima-se que mais de 80% dos cânceres de mama que metastatizam para o TGF são CLI. Um estudo realizado em mulheres com metástase ginecológica (n=54) demonstrou que 42,6% tinham câncer de mama correspondente a CLI; 48,1%, carcinoma invasor sem tipo específico; e 9,3%, outros. Além disso, o estudo revelou que os sítios de metástases ginecológicas mais envolvidos foram os ovários, seguido das tubas e do útero. A respeito da expressão de biomarcadores de tumores primários que se espalharam para sítios ginecológicos, 93,5% expressou RE positivo; 65,7%, RP positivo; e 0%, HER2 positivo. Uma avaliação de 13 casos mostrou que, enquanto a expressão de RE e FOXA1 foi mantida ou aumentada durante a progressão para órgãos ginecológicos, a expressão de proteínas acessórias envolvidas com a regulação hormonal diminuiu. Conforme o aumento do tumor, as células que expressam RP, RA e GATA3 em metástases distantes diminuíram em 69,2, 38,5 e 46% dos casos, respectivamente. O relato em questão confirma os resultados obtidos nos estudos, já que apresenta semelhança na expressão gênica de carcinomas primários de mama com metástase para o TGF. No entanto, apresenta um histotipo incomum para metástases ginecológicas.