Journal articles: 'Cisgenesi'

1

Eriksson, Dennis, Sten Stymne, and Jan K. Schjoerring. "The slippery slope of cisgenesis." Nature Biotechnology 32, no. 8 (August 2014): 727. http://dx.doi.org/10.1038/nbt.2980.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Shankar, A. G. "Cisgenesis: An Approach for Crop Improvement." International Journal of Pure & Applied Bioscience 5, no. 3 (July 30, 2017): 245–50. http://dx.doi.org/10.18782/2320-7051.4041.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Schouten, Henk. "Reply to The slippery slope of cisgenesis." Nature Biotechnology 32, no. 8 (August 2014): 728. http://dx.doi.org/10.1038/nbt.2981.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Schouten, Henk J., and Evert Jacobsen. "Cisgenesis and intragenesis, sisters in innovative plant breeding." Trends in Plant Science 13, no. 6 (June 2008): 260–61. http://dx.doi.org/10.1016/j.tplants.2008.04.005.

Full text

APA, Harvard, Vancouver, ISO, and other styles

5

Espinoza, C., R. Schlechter, D. Herrera, E. Torres, A. Serrano, C. Medina, and P. Arce-Johnson. "Cisgenesis and Intragenesis: New tools For Improving Crops." Biological Research 46, no. 4 (2013): 323–31. http://dx.doi.org/10.4067/s0716-97602013000400003.

Full text

APA, Harvard, Vancouver, ISO, and other styles

6

Holme, Inger Baeksted, Toni Wendt, and Preben Bach Holm. "Intragenesis and cisgenesis as alternatives to transgenic crop development." Plant Biotechnology Journal 11, no. 4 (February 20, 2013): 395–407. http://dx.doi.org/10.1111/pbi.12055.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

DUDZIAK, KAROLINA, MAGDALENA SOZONIUK, KRZYSZTOF KOWALCZYK, and MICHAŁ NOWAK. "Cisgenesis as a novel prospect for crop improvement. A review." Agronomy Science 74, no. 2 (September 4, 2019): 7–14. http://dx.doi.org/10.24326/as.2019.2.1.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

den Nijs, T., H. Schouten, and F. Krens. "CISGENESIS FITS IN THE TOOLKIT OF A MODERN FRUIT BREEDER." Acta Horticulturae, no. 976 (February 2013): 435–38. http://dx.doi.org/10.17660/actahortic.2013.976.60.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Jacobsen, E., and H. J. Schouten. "Cisgenesis: an important sub-invention for traditional plant breeding companies." Euphytica 170, no. 1-2 (September 21, 2009): 235–47. http://dx.doi.org/10.1007/s10681-009-0037-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Jacobsen, Evert, and Henk J. Schouten. "Cisgenesis strongly improves introgression breeding and induced translocation breeding of plants." Trends in Biotechnology 25, no. 5 (May 2007): 219–23. http://dx.doi.org/10.1016/j.tibtech.2007.03.008.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Jochemsen, H. "An Ethical Assessment of Cisgenesis in Breeding Late Blight Resistant Potato." Potato Research 51, no. 1 (March 2008): 59–73. http://dx.doi.org/10.1007/s11540-008-9090-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Galarza, Janeth I., Ninoska Delgado, and Vitalia Henríquez. "Cisgenesis and intragenesis in microalgae: promising advancements towards sustainable metabolites production." Applied Microbiology and Biotechnology 100, no. 24 (November 7, 2016): 10225–35. http://dx.doi.org/10.1007/s00253-016-7948-z.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Han, Katherine M., Palitha Dharmawardhana, Reneé S. Arias, Cathleen Ma, Victor Busov, and Steven H. Strauss. "Gibberellin-associated cisgenes modify growth, stature and wood properties in Populus." Plant Biotechnology Journal 9, no. 2 (January 6, 2011): 162–78. http://dx.doi.org/10.1111/j.1467-7652.2010.00537.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Mlalazi, Bulukani, Ralf Welsch, Priver Namanya, Harjeet Khanna, R. Jason Geijskes, Mark D. Harrison, Rob Harding, James L. Dale, and Marion Bateson. "Isolation and functional characterisation of banana phytoene synthase genes as potential cisgenes." Planta 236, no. 5 (July 29, 2012): 1585–98. http://dx.doi.org/10.1007/s00425-012-1717-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Raymond, Guillaume, Martin Blais, Félix-Antoine Bergeron, and Martine Hébert. "Les expériences de victimisation, la santé mentale et le bien-être de jeunes trans au Québec." Santé mentale au Québec 40, no. 3 (January 29, 2016): 77–92. http://dx.doi.org/10.7202/1034912ar.

Full text

Abstract:

Les normes traditionnelles de genre prescrivent l’adéquation de l’expression de la masculinité et de la féminité au sexe anatomique de naissance. Les personnes qui présentent des variations dans l’expression de genre sont sujettes à diverses formes de réactions sociales suggérant la réprobation (des regards désapprobateurs aux violences physiques) susceptibles d’influencer négativement leur santé mentale. Trente-sept jeunes se décrivant comme trans ou en questionnement sur leur identité de genre ont été recrutés dans le cadre de l’enquête sur les Parcours amoureux des jeunes de minorités sexuelles du Québec. Leurs expériences de victimisation parentale et de victimisation basée sur la non-conformité de genre ainsi que des indicateurs de santé mentale (détresse psychologique, estime de soi) ont été mesurés. Afin de comparer les expériences de victimisation et l’état de santé mentale des jeunes trans, ils ont été appariés à 37 garçons et 37 filles cisgenres sur la base de leurs caractéristiques sociodémographiques. Des analyses de prévalence et un modèle acheminatoire ont été réalisés. Les résultats mettent en évidence que les jeunes trans sont plus susceptibles de présenter des scores cliniques de détresse psychologique et de faible estime d’eux-mêmes que leurs pairs cisgenres. La violence verbale parentale et la victimisation basée sur la non-conformité de genre influencent négativement l’estime de soi, ce qui en retour augmente la probabilité de vivre de la détresse psychologique. Les résultats appuient l’importance des interventions de soutien à la diversité sexuelle et de genre.

APA, Harvard, Vancouver, ISO, and other styles

16

Ahmad, Daraz, Zhongwei Zhang, Haroon Rasheed, Xiaoyong Xu, and Jinsong Bao. "Recent Advances in Molecular Improvement for Potato Tuber Traits." International Journal of Molecular Sciences 23, no. 17 (September 1, 2022): 9982. http://dx.doi.org/10.3390/ijms23179982.

Full text

Abstract:

Potato is an important crop due to its nutritional value and high yield potential. Improving the quality and quantity of tubers remains one of the most important breeding objectives. Genetic mapping helps to identify suitable markers for use in the molecular breeding, and combined with transgenic approaches provides an efficient way for gaining desirable traits. The advanced plant breeding tools and molecular techniques, e.g., TALENS, CRISPR-Cas9, RNAi, and cisgenesis, have been successfully used to improve the yield and nutritional value of potatoes in an increasing world population scenario. The emerging methods like genome editing tools can avoid incorporating transgene to keep the food more secure. Multiple success cases have been documented in genome editing literature. Recent advances in potato breeding and transgenic approaches to improve tuber quality and quantity have been summarized in this review.

APA, Harvard, Vancouver, ISO, and other styles

17

Schouten, H. J., J. M. Soriano, S. G. Joshi, A. J. Kortstee, F. A. Krens, J. G. Schaart, K. van der Linden, et al. "CISGENESIS IS A PROMISING APPROACH FOR FAST, ACCEPTABLE AND SAFE BREEDING OF PIP FRUIT." Acta Horticulturae, no. 814 (March 2009): 199–204. http://dx.doi.org/10.17660/actahortic.2009.814.26.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Salimbeni, Alice. "La favola urbana. Reimmaginare lo spazio attraverso la realizzazione collettiva di film finzionali e parodici." RIVISTA GEOGRAFICA ITALIANA, no. 3 (September 2022): 78–102. http://dx.doi.org/10.3280/rgioa3-2022oa14591.

Full text

Abstract:

Durante la mia ricerca di dottorato, ho organizzato un workshop a Bruxelles per esplorare il rapporto fra le donne bianche cisgenere e lo spazio urbano. A partire dalle esperienze urbane personali delle partecipanti abbiamo scritto tre storie di invenzione e le abbiamo trasformate in tre film collettivi, finzionali e parodici che esplorano ciascuno una diversa discriminazione urbana di genere. In queste storie, che ho chiamato "favole urbane", realtà e immaginazione si intrecciano creando nuovi scenari, e comunicando una morale politica sullo spazio e sul ruolo attivo che svolge nella produzione delle discriminazioni.

APA, Harvard, Vancouver, ISO, and other styles

19

Louwaars, Niels, and Henk Jochemsen. "An Ethical and Societal Analysis for Biotechnological Methods in Plant Breeding." Agronomy 11, no. 6 (June 10, 2021): 1183. http://dx.doi.org/10.3390/agronomy11061183.

Full text

Abstract:

Technological developments in plant breeding, notably cisgenesis and gene editing, require a rethinking of biotechnology policies. In addition to legal debates about the definition of genetic modification in the Cartagena Protocol and at national and supra-national levels, and debates about the safety of the resulting products for mankind and environment, discussions are ongoing in society concerning ethical and societal questions. In this paper, we analyse the main ethical issues that need to be taken into account when evaluating contemporary plant breeding techniques. After a brief description of the state of the art in plant breeding, we discuss these main ethical issues. We take Consequentialist, Deontological and Virtue ethics as bases of our analysis. This results in a generally positive approach to gene editing, but also highlights several concerns, predominantly used by particular groups in society. This leads to a moral incentive toward transparency and options for operationalizing consumer choice.

APA, Harvard, Vancouver, ISO, and other styles

20

Lammerts van Bueren, E. T., M. Tiemens-Hulscher, and P. C. Struik. "Cisgenesis Does Not Solve the Late Blight Problem of Organic Potato Production: Alternative Breeding Strategies." Potato Research 51, no. 1 (March 2008): 89–99. http://dx.doi.org/10.1007/s11540-008-9092-3.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Jo, Kwang-Ryong, Chol-Jun Kim, Sung-Jin Kim, Tok-Yong Kim, Marjan Bergervoet, Maarten A. Jongsma, Richard GF Visser, Evert Jacobsen, and Jack H. Vossen. "Development of late blight resistant potatoes by cisgene stacking." BMC Biotechnology 14, no. 1 (2014): 50. http://dx.doi.org/10.1186/1472-6750-14-50.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Fedorina, Jaroslava, Nadezhda Tikhonova, Yulia Ukhatova, Roman Ivanov, and Elena Khlestkina. "Grapevine Gene Systems for Resistance to Gray Mold Botrytis cinerea and Powdery Mildew Erysiphe necator." Agronomy 12, no. 2 (February 17, 2022): 499. http://dx.doi.org/10.3390/agronomy12020499.

Full text

Abstract:

Grapevine is one of the world’s most economically important fruit crops. It is known that Vitis vinifera is a host for a large number of pathogenic agents, which significantly reduce the yield and berry quality. This forces the agronomists to use a huge amount of fungicides. Over the last few decades, alternative methods for solving this problem have been developed and continue to be developed. Such new technologies as marker-assisted selection, bioengineering of the rhizosphere, genetic engineering (transgenesis, cisgenesis and intragenesis) allow the production of pathogen-resistant cultivars. However, they are linked to a number of problems. One of the most promising methods is the creation of modified non-transgenic cultivars via CRISPR/Cas9-targeted mutagenesis. Therefore, researchers are actively looking for target genes associated with pathogen resistance and susceptibility. This review elucidates the main mechanisms of plant—pathogen interactions, the immune systems developed by plants, as well as the identified genes for resistance and susceptibility to the biotrophic pathogen Erysiphe necator and the necrotrophic pathogen Botrytis cinerea.

APA, Harvard, Vancouver, ISO, and other styles

23

Park, T. H., V. G. A. A. Vleeshouwers, E. Jacobsen, E. van der Vossen, and R. G. F. Visser. "Molecular breeding for resistance toPhytophthora infestans(Mont.) de Bary in potato (Solanum tuberosumL.): a perspective of cisgenesis." Plant Breeding 128, no. 2 (April 2009): 109–17. http://dx.doi.org/10.1111/j.1439-0523.2008.01619.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Carr, John N. "Skateboarding in Dude Space: The Roles of Space and Sport in Constructing Gender Among Adult Skateboarders." Sociology of Sport Journal 34, no. 1 (March 2017): 25–34. http://dx.doi.org/10.1123/ssj.2016-0044.

Full text

Abstract:

This study aims to address how, to what extent, and under what conditions may those who are not cisgendered as male do the work of negotiating access to male sporting space. In doing so, it brings together critical geographies of masculinity and the critical literature on skateboarding to address the role of particular kinds of skateboarding spaces in either reproducing or potentially disrupting gender segregated, patriarchal skateboarding cultures. This project is offered not only to challenge patriarchal practices and values, but also to step beyond theory and actually examine how sport environments might be designed and sited so as to enable a wider range of gender performances and more inclusive spaces. Specifically, my research suggests that certain types of skate environments can somewhat lower women’s barriers to entering the gender charged realm of skateboarding if and when those responsible for those spaces take patriarchy and the needs of noncisgendered male skateboarders seriously.Cette étude a pour but de savoir comment, dans quelle mesure et dans quelles conditions, les personnes n’étant pas des hommes cisgenres négocient leur accès dans l’univers sportif masculin. Pour ce faire, elle réunit les géographies critiques de la masculinité et la littérature critique sur le skateboard pour examiner le rôle de certains univers particuliers de cette pratique dans la reproduction, ou dans l’éventuelle rupture avec la ségrégation genrée, des cultures patriarcales du skateboard. Le but de ce projet n’est pas seulement de contester les pratiques et les valeurs patriarcales, mais aussi de dépasser la théorie et d’examiner vraiment comment les environnements sportifs pourrait être conçus et localisés de manière à permettre une plus grande variété de performances en fonction du genre et de devenir des espaces plus inclusifs. Plus spécifiquement, ma recherche suggère que certains types d’environnements dédiés à la glisse pourraient contribuer à réduire les barrières que rencontrent les femmes pour entrer dans la sphère fortement genrée du skateboard si et quand les responsables de ces espaces prendront sérieusement en compte le patriarcat et les besoins des skatebordeurs qui ne sont pas cisgenres.

APA, Harvard, Vancouver, ISO, and other styles

25

Cardi, Teodoro. "Cisgenesis and genome editing: combining concepts and efforts for a smarter use of genetic resources in crop breeding." Plant Breeding 135, no. 2 (February 1, 2016): 139–47. http://dx.doi.org/10.1111/pbr.12345.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Islam, M. S., M. Z. K. Roni, R. Mondal, and K. Shimasaki. "Cis vs. trans: is cisgenesis an attractive alternative for transgenesis to make genetic modification acceptable to the public?" Acta Horticulturae, no. 1297 (November 2020): 423–32. http://dx.doi.org/10.17660/actahortic.2020.1297.56.

Full text

APA, Harvard, Vancouver, ISO, and other styles

27

Beghin, John C., and Christopher R. Gustafson. "Consumer Valuation of and Attitudes towards Novel Foods Produced with New Plant Engineering Techniques: A Review." Sustainability 13, no. 20 (October 14, 2021): 11348. http://dx.doi.org/10.3390/su132011348.

Full text

Abstract:

We follow the PRISMA extension for scoping reviews to review the emerging international body of empirical evidence on consumers’ attitudes and willingness to pay (WTP) for novel foods produced with New Plant Engineering Techniques (NPETs). NPETs include genome/gene editing, cisgenesis, intragenesis, and RNA interference. These novel foods are often beneficial for the environment and human health and more sustainable under increasingly prevalent climate extremes. These techniques can also improve animal welfare and disease resistance when applied to animals. Despite these abilities of NPETs, evidence suggests that many, but not all, consumers discount these novel foods relative to conventional ones. Our review sorts out findings to identify conditioning factors that can increase the acceptance of and WTP for these novel foods in a significant segment of consumers. International patterns of acceptance are identified. We also analyze how information and knowledge interact with consumer acceptance of these novel foods and technologies. Heterogeneity of consumers—across cultures and borders and in attitudes towards science and innovation—emerges as a key determinant of acceptance and WTP. Acceptance and WTP tend to increase when socially beneficial attributes—as opposed to producer-oriented cost-saving attributes—are generated by NPETs. NPET-improved foods are systematically less discounted than transgenic foods. Most of the valuation estimates are based on hypothetical experiments and surveys and await validation through revealed preferences in actual purchases in food retailing environments.

APA, Harvard, Vancouver, ISO, and other styles

28

Haverkort, A. J., P. M. Boonekamp, R. Hutten, E. Jacobsen, L. A. P. Lotz, G. J. T. Kessel, J. H. Vossen, and R. G. F. Visser. "Durable Late Blight Resistance in Potato Through Dynamic Varieties Obtained by Cisgenesis: Scientific and Societal Advances in the DuRPh Project." Potato Research 59, no. 1 (January 25, 2016): 35–66. http://dx.doi.org/10.1007/s11540-015-9312-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Hakeem, Hasheem. "Axiomes de la pédagogie queer." Voix Plurielles 18, no. 2 (December 4, 2021): 261–73. http://dx.doi.org/10.26522/vp.v18i2.3411.

Full text

Abstract:

Cet article présente une synthèse des résultats d’une recherche qui a analysé les perceptions d’élèves du secondaire au sujet de la diversité sexuelle et de genre. L’étude de cas qualitative a été menée auprès de 24 participant.e.s (âgé.e.s de 17 à 18 ans) provenant d’une école secondaire du Grand Vancouver. L’analyse de leurs discours a révélé que leurs perceptions avaient tendance à réaffirmer des visions du monde hétéronormatives et cisgenres, à normaliser la diversité sexuelle et de genre, et à minimiser l’impact des idéologies homophobes et transphobes en tant que formes de discrimination systémique. Les participants garçons étaient également plus susceptibles de défendre des conceptions binaires et biologiques du genre, et de rejeter l’identité trans, suggérant un lien entre la performance sociale de la transphobie et la construction de la masculinité cishétéronormative. À partir de ces résultats, une série de huit axiomes sont présentés dans le but d’orienter le développement d’une pratique pédagogique queer et de contribuer ainsi à la création de salles de classe véritablement équitables, inclusives et critiques.

APA, Harvard, Vancouver, ISO, and other styles

30

Rousselière, Damien, and Samira Rousselière. "Is biotechnology (more) acceptable when it enables a reduction in phytosanitary treatments? A European comparison of the acceptability of transgenesis and cisgenesis." PLOS ONE 12, no. 9 (September 6, 2017): e0183213. http://dx.doi.org/10.1371/journal.pone.0183213.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Maltseva, Elina, Gulnur Iskakova, Ainur Ismagul, Alexandr Chirkin, Dinara Naizabayeva, Gulnara Ismagulova, Natalya Malakhova, Nagima Aitkhozhina, Serik Eliby, and Yuriy Skiba. "A Cisgenic Approach in the Transformation of Bread Wheat cv. Saratovskaya 29 with Class I Chitinase Gene." Open Biotechnology Journal 15, no. 1 (May 24, 2021): 29–35. http://dx.doi.org/10.2174/1874070702115010029.

Full text

Abstract:

Background: Bread wheat is one of the major crops grown worldwide, showing high demand for new varieties with traits such as pathogen resistance. As the public acceptance of transgenic plants remains low, a novel approach – cisgenesis – is being developed to introduce the genes from the same or closely related species. Objective: This study presents a cisgenic approach used for the transformation of wheat with class I chitinase gene derived from T. aestivum cv. Stepnaya 15, co-transformed with acetohydroxyacid synthase gene that provides tolerance to imidazolinone herbicides. Methods: Calli from immature embryos of spring bread wheat Triticum aestivum cv. Saratovskaya 29 were used for co-transformation with two independent Minimal Expression Units (MEUs): class I chitinase and Acetohydroxyacid Synthase (AHAS) gene. For identification of cisgenic plants, genomic DNA was extracted from the leaves of imazethapyr-resistant regenerant plants at the plantlets stage and screened by polymerase chain reaction. The efficiency of transformation was calculated as the relation of regenerated plants with chitinase gene insert to the total number of calli in the experiment. Results: The average transformation efficiency in four series of experiments (total number of calli - 2299) was found to be 1.84% (ranging from 0.3% to 3.4%), while total co-transformation efficiency reached 87.93%. Conclusion: The high efficiency of co-transformation in the experiment promotes it as a very useful technique for the production of wheat lines, free of the selectable marker gene. To our knowledge, this is the first report of cisgenic bread wheat, where both target and selectable genes are derived from wheat.

APA, Harvard, Vancouver, ISO, and other styles

32

Pagliarini, Renata Fuganti, Juliane Prela Marinho, Mayla Daiane Correa Molinari, Juliana Marcolino-Gomes, André Luís Hartmann Caranhoto, Silvana Regina Rockenbach Marin, Maria Cristina Neves Oliveira, et al. "Overexpression of full-length and partial DREB2A enhances soybean drought tolerance." Agronomy Science and Biotechnology 8 (September 16, 2021): 1–21. http://dx.doi.org/10.33158/asb.r141.v8.2022.

Full text

Abstract:

Soybean is an important commodity worldwide. Abiotic conditions can adversely disturb crop growth and final yield. The transcription factor Dehydration-Responsive Element-Binding Proteins 2 (DREB2) act as a regulator of drought-responses. This study aimed to characterize soybean plants genetically modified with GmDREB2A;2 FL and GmDREB2A;2 CA for molecular, physiological, and agronomic responses, at different developmental periods. Results showed that seedlings from GmDREB2A;2 FL event presented lower growth reduction under osmotic treatment during germination. The GmDREB2A;2 FL and GmDREB2A;2 CA events showed improved performance in experiments of water deficit imposed in the vegetative period and higher rates in physiological parameters. In the reproductive period, there was a trend of higher yield compounds in GM GmDREB2A;2 FL event when compared to other genotypes and treatments. It was suggested that GmDREB2A;2 FL event presented superior performance due to the higher expression levels of the cisgene and drought-induced genes.

APA, Harvard, Vancouver, ISO, and other styles

33

Lütken, Henrik, Marina Laura, Cristina Borghi, Marian Ørgaard, Andrea Allavena, and Søren K. Rasmussen. "Expression of KxhKN4 and KxhKN5 genes in Kalanchoë blossfeldiana ‘Molly’ results in novel compact plant phenotypes: towards a cisgenesis alternative to growth retardants." Plant Cell Reports 30, no. 12 (August 18, 2011): 2267–79. http://dx.doi.org/10.1007/s00299-011-1132-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Sabbadini, S., L. Capriotti, C. Limera, O. Navacchi, G. Tempesta, and B. Mezzetti. "A plant regeneration platform to apply new breeding techniques for improving disease resistance in grapevine rootstocks and cultivars." BIO Web of Conferences 12 (2019): 01019. http://dx.doi.org/10.1051/bioconf/20191201019.

Full text

Abstract:

Worldwide grapevine cultivation is based on the use of elite cultivars, in many cases strictly linked to local important wine brands. Most of Vitis viniferacultivars have high susceptibility to fungal and viral diseases therefore, new breeding techniques (e.g. Cisgenesis, RNAi and gene editing) offer the possibility to introduce new clones of the main cultivars with increased diseases resistance, in order to reduce environmental impact and improve quality in the intensive wine grape industry. This study is finalized to develop efficient in vitro regeneration and transformation protocols to extend the application of these technologies in wine grape cultivars and rootstocks. With this aim, in vitro regeneration protocols based on the production of meristematic bulks (Mezzetti et al., 2002) were optimized for different grapevine cultivars (Glera, Vermentino, Sangiovese, Thompson Seedless) and rootstocks (1103 Paulsen, and 110 Richter). The meristematic bulks were then used as explants for Agrobacteriummediated genetic transformation protocols, by comparing the use of NPTII and e-GFP as marker genes. Results confirmed the efficiency of meristematic bulks as the regenerating tissue to produce new modified plants in almost all the above genotypes. The highest regeneration efficiency in some genotypes allowed the selection of stable modified lines/calli with only the use of e-GFP marker gene. This protocol can be applied in the use of MYB marker gene for the production of cisgenic lines. Genotypes having the highest regeneration and transformation efficiency were also used for transformation experiments using a hairpin gene construct designed to silence the RNA-dependent RNA polymerase (RpRd) of the GFLV and GLRaV3, which would induce multiple virus resistances, and the Dicer-like protein 1 (Bc-DCL1) and Bc-DCL2 to control B. cinerea infection.

APA, Harvard, Vancouver, ISO, and other styles

35

Jacobsen, E., and H. J. Schouten. "Cisgenesis, a New Tool for Traditional Plant Breeding, Should be Exempted from the Regulation on Genetically Modified Organisms in a Step by Step Approach." Potato Research 51, no. 1 (March 2008): 75–88. http://dx.doi.org/10.1007/s11540-008-9097-y.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Muratova (Fadina), O. A., M. P. Beketova, M. A. Kuznetsova, E. V. Rogozina, and E. E. Khavkin. "South American species Solanum alandiae Card. and S. okadae Hawkes et Hjerting as potential sources of genes for potato late blight resistance." Proceedings on applied botany, genetics and breeding 181, no. 1 (April 12, 2020): 73–83. http://dx.doi.org/10.30901/2227-8834-2020-1-73-83.

Full text

Abstract:

For several decades, wild species of Solanum L. section Petota Dumort. have been involved in potato cultivar breeding for robust resistance to pests and diseases. Potato late blight (LB) is caused by oomycete Phytophthora infestans (Mont.) de Bary, and the genes for race-specific resistance to P. infestans (Rpi genes) have been introgressed into cultivated potatoes by remote crosses and trans- or cisgenesis, first from S. demissum Buk. and, more recently, from other wild species, such as S. bulbocastanum Dun., S. stoloniferum Schlechtd. et Bché, and S. venturii Hawkes et Hjerting (according to the nomenclature by Hawkes, 1990). Most wild species already involved in breeding for LB resistance came from North and Central Americas: series Bulbocastana (Rydb.) Hawkes, Demissa Buk. and Longipedicellata Buk., and some Rpi genes of these species have been already characterized in much detail. Rpi genes of South American species, including the series Tuberosa (Rydb.) Hawkes, have not been sufficiently investigated. Among the latter, this study focuses on the Rpi genes of S. alandiae Card. and S. okadae Hawkes et Hjerting. Four accessions of S. alandiae, one accession of S. okadae and 11 clones of interspecific potato hybrids comprising S. alandiae germplasm from the VIR collection were PCR-screened using specific SCAR (Sequence Characterized Amplified Region) markers for eight Rpi genes. SCAR amplicons of five Rpi genes registered in this study were validated by comparing their sequences with those of prototype genes deposited in the NCBI Genbank. Among the structural homologues of Rpi genes found in S. alandiae and S. okadae, of special interest are homologues of CC-NB-LRR resistance genes with broad specificity towards P. infestans races, in particular R2=Rpi-blb3, R8, R9a, Rpi-vnt1 and Rpi-blb2 (94–99, 94–99, 86–89, 92–98 and 91% identity with the prototype genes, respectively). Our data may help to better understand the process of Rpi gene divergence along with the evolution of tuberbearing Solanum species, particularly in the series Tuberosa.

APA, Harvard, Vancouver, ISO, and other styles

37

Pontes, Alice Fonseca, Talita Bianca Lima da Paixão, João Victor Barbosa dos Santos, Natália Almeida Rodrigues, Maria Eduarda da Silva Bastos, Amanda Monteiro dos Santos, Sanmyra Lopes Araújo, Rafaela Ribeiro de Oliveira Estelita, Simone Fernandes Monteiro, and Ângela Roberta Lessa de Andrade. "Papel da enfermagem no cuidado a mulheres profissionais do sexo." Research, Society and Development 11, no. 16 (November 30, 2022): e92111638002. http://dx.doi.org/10.33448/rsd-v11i16.38002.

Full text

Abstract:

Viu-se a necessidade de explanar o papel da enfermagem no cuidado e a atenção integral voltada para as profissionais do sexo, a fim de se diminuir riscos e malefícios para saúde dessas mulheres, tendo como objetivo descrever sobre a atenção integral voltada para mulheres profissionais do sexo e o papel da enfermagem no cuidado a esta população. Os dados foram colhidos de 13 artigos filtrados na BVS em duas bases de dados: LILACS e a BDENF acerca das mulheres profissionais do sexo. Foram utilizados o NANDA edição 2018-2020 e o NIC 6º Edição para realização dos Diagnósticos de Enfermagem e intervenções. Os resultados obtidos relatam que o perfil sociodemográfico dessa população colabora para o negligenciamento, pois trata-se em grande parte de mulheres negras cisgenero e/ou transsexuais, com baixo nível de escolaridade, integrantes de classe sociais menos abastadas e financeiramente instáveis, além disso, comumente encontram obstáculos por estas no acesso à saúde. Soma-se ainda a questão atual da pandemia causada pelo vírus SARS-CoV-2 que tornou mais difícil a atuação profissional dessas mulheres, agravando ainda mais a situação de vulnerabilidade. A partir dos Diagnósticos do NANDA-I e das intervenções do NIC, é possível guiar um cuidado de enfermagem integral, equânime e eficiente para esse público.É fundamental que a enfermagem desenvolva um olhar holístico, desprendido de preconceitos para desenvolver um cuidado que atenda às necessidades humanas básicas das profissionais do sexo, com a prestação de serviço pautada na ética profissional, aliada ao respeito e responsabilidade para com estas mulheres.

APA, Harvard, Vancouver, ISO, and other styles

38

Tarasuk, Jill, Meghan Sullivan, Donna Bush, Christian Hui, Melissa Morris, Tami Starlight, François Cholette, et al. "Résultats en ce qui concerne les participants autochtones sondés dans le cadre de l'enquête Track auprès des utilisateurs de drogues injectables au Canada, phase 4, 2017 à 2019." Relevé des maladies transmissibles au Canada 47, no. 1 (January 29, 2021): 43–55. http://dx.doi.org/10.14745/ccdr.v47i01a07f.

Full text

Abstract:

Contexte : L’enquête Track auprès des utilisateurs de drogues injectables a permis de recueillir des données dans quatorze sites sentinelles au Canada (2017 à 2019). Ces résultats décrivent la prévalence du virus de l’immunodéficience humaine (VIH), de l’hépatite C et des comportements à risque associés à ceux-ci chez les participants autochtones. Méthodes : Des informations sur les caractéristiques sociodémographiques, les déterminants sociaux de la santé, le recours aux services de prévention et au dépistage, la consommation de drogues, les comportements à risque, ainsi que le dépistage, les soins et le traitement du VIH et de l’hépatite C ont été recueillies par l’entremise de questionnaires administrés par un intervieweur. Les échantillons biologiques ont été analysés pour y détecter la présence d’anticorps anti-VIH et anti-hépatite C et l’acide ribonucléique (ARN) de l’hépatite C. Les statistiques descriptives ont été calculées et examinées par un groupe consultatif dirigé par des autochtones, selon l’approche à double perspective (Two-Eyed Seeing). Résultats : Parmi les 2 383 participants, 997 étaient des autochtones (82,9 % étaient des membres des Premières Nations, 14,9 % étaient des Métis et 2,2 % étaient des Inuits). Plus de la moitié (54,5 %) étaient des hommes cisgenres et l’âge moyen était de 38,9 ans. Une grande proportion (84,0 %) des participants ont déclaré que leur santé mentale était de « passable excellente ». Une forte proportion d’entre eux ont été victimes de stigmatisation et de discrimination (90,2 %) ainsi que de violences physiques, sexuelles et/ou psychologiques durant l’enfance (87,5 %) ou de la part d’un partenaire sexuel (78,6 %). Un pourcentage élevé d’entre eux ont déclaré utiliser un programme de distribution de seringues (90,5 %) et avoir été dépisté pour le VIH (87,9 %) et l’hépatite C (87,8 %). La prévalence du VIH était de 15,4 % (78,2 % d’entre eux avaient connaissance de leur statut infectieux) et 36,4 % d’entre eux étaient séropositifs pour l’ARN de l’hépatite C (49,4 % d’entre eux avaient connaissance de leur statut infectieux). Conclusion : L’enquête a révélé des taux élevés de VIH et d’hépatite C. Elle a également révélé des défis liés à l’accès et au maintien des soins et des traitements liés au VIH et à l’hépatite C. Ces renseignements éclairent les stratégies de réduction des méfaits, y compris la nécessité d’accroître la sensibilisation à la prophylaxie d’une manière culturellement pertinente.

APA, Harvard, Vancouver, ISO, and other styles

39

Lydon-Hassen, Kathleen, Leigh Jonah, Lisa Mayotte, Ashley Hrabowy, Bonny Graham, Beverley Missens, Amanda Nelson, et al. "Sommaire des résultats des enquêtes Tracks mises en œuvre par les Premières Nations en Saskatchewan et en Alberta, au Canada, de 2018 à 2020." Relevé des maladies transmissibles au Canada 48, no. 4 (April 6, 2022): 163–75. http://dx.doi.org/10.14745/ccdr.v48i04a05f.

Full text

Abstract:

Contexte : Le système intégré de surveillance biocomportementale de l’Agence de la santé publique du Canada — Enquêtes Tracks — évalue le fardeau du VIH, de l’hépatite C et des risques associés dans les populations clés au Canada. De 2018 à 2020, les enquêtes Tracks ont été mises en œuvre avec succès par les organisations de services de santé dirigées par les Premières Nations en Alberta et en Saskatchewan. Méthodes : Les équipes responsables des enquêtes dirigées par les Premières Nations ont invité les membres de la collectivité qui s’identifiaient comme membres des Premières Nations, des Inuits ou des Métis à participer aux enquêtes Tracks et au dépistage du VIH, de l’hépatite C et de la syphilis. Des informations ont été recueillies sur les déterminants sociaux de la santé, le recours aux services de prévention, la consommation de substances, les comportements sexuels et les soins du VIH et de l’hépatite C. Des statistiques descriptives sont présentées. Résultats : Parmi les 1 828 participants, 97,4 % se sont identifiés comme membres des Premières Nations et 91,4 % vivaient dans une collectivité située dans une réserve. Plus de la moitié (52,2 %) étaient des femmes cisgenres, l’âge moyen était de 36,3 ans, 82,5 % vivaient dans un logement stable, 82 % avaient accès à des soins de santé primaires et 73,8 % ont déclaré avoir une bonne ou excellente santé mentale. La plupart des participants (97 %) avaient un membre de leur famille qui avait été placé dans un pensionnat. Des proportions élevées ont été victimes de stigmatisation et de discrimination (65,6 %), de contraintes financières (64,3 %) et d’abus dans l’enfance (65,1 %). Le dépistage du VIH (62,8 %) et de l’hépatite C (55,3 %) était relativement élevé. La prévalence du VIH était de 1,6 % (dont 64 % connaissaient leur statut d’infection). La prévalence de l’acide ribonucléique de l’hépatite C était de 5 % (44,9 % des personnes connaissaient leur statut d’infection actuel). Conclusion : Des expériences historiques et continues de traumatismes et une prévalence plus élevée de l’hépatite C ont été déterminées, réaffirmant les données probantes du legs du colonialisme, des pensionnats autochtones et du racisme systémique. La participation élevée au dépistage et à la prévention des infections transmissible sexuellement et par le sang (ITSS) reflète l’importance des services et des programmes de soins de santé adaptés à la culture, sûrs et réactifs, dirigés par les Premières Nations, pour obtenir de meilleurs résultats pour les peuples des Premières Nations.

APA, Harvard, Vancouver, ISO, and other styles

40

Antonova, O. Y., N. S. Klimenko, Z. Z. Evdokimova, L. I. Kostina, and T. A. Gavrilenko. "Finding RB/Rpi-blb1/Rpi-sto1-like sequences in conventionally bred potato varieties." Vavilov Journal of Genetics and Breeding 22, no. 6 (September 27, 2018): 693–702. http://dx.doi.org/10.18699/vj18.412.

Full text

Abstract:

The main objectives in potato breeding are increasing yield abilities and improving resistance to numerous pathogens and pests. Among them, the late blight caused by the Phytophthora infestans oomycete is one of the most destructive potato diseases both in Russia and worldwide. Wild relatives of cultivated potato are traditionally used in breeding as the source of valuable R genes conferring resistance to pathogens. Of particular interest are Mexican wild species because Mexico is the centre of origin and diversity of P. infestans and at the same time, it is the centre of potato species diversity. Mexican wild potato species S. bulbocastanum and S. stoloniferum are an important source of the R genes conferring broad-spec trum resistance against various isolates of P. infestans (Rpi-blb1, Rpi-blb2, Rpi-sto1). Recently these genes have been transferred into cultivated potato gene pool using the cisgene approach. At the same time there is a high probability of finding geno types with the Rpi-sto1 gene (functional homologues of Rpi-blb1) among conventionally bred varieties because for about 40 years S. stoloniferum has been used in breeding as a source of the Rysto and Ry-fsto genes of the extreme resistance to the most important viral pathogen PVY. In this study 188 potato varieties bred in Russia and in near-abroad countries were screened for the presence of six gene-specific markers of the RB/Rpi-blb1 = Rpi-sto1 and Rpi-blb2 genes conferring broad-spectrum resistance against P. infestans, and for the markers linked to the Rysto and Ry-fsto genes conferring extreme resistance to PVY. In addition, a marker for detecting male sterile mitochondrial DNA type gamma derived from S. stoloniferum was used. The genotypes selected through the molecular markers were divided into four groups: (A) 13 PVY resistant varieties carrying diagnostic markers of the Rysto, Ry-fsto genes and having sterile mt-type gamma; (B) four varieties possessing mt-type gamma and not having the markers of the R genes introgressed from S. stoloniferum; (C) eight genotypes carrying five gene-specific markers for the RB/Rpi-blb1/= Rpi-sto1; (D) the rest 166 (86.9 %) varieties not possessing any of the diagnostic markers associated with the S. stoloniferum genetic material. The sequences of the Rpi-sto1- and BLB1 F/R-amplicons were identical in all the genotypes of group ‘C’ and showed respective 99 % and 100 % similarity to the corresponding fragments of the Rpi-sto1 and Rpi-blb1 genes from the GenBank database. Among the genotypes of group ‘C’ various mt-types were detected, and some of them were male fertile.

APA, Harvard, Vancouver, ISO, and other styles

41

Xu, Wenting, Hanwen Zhang, Yuchen Zhang, Ping Shen, Xiang Li, Rong Li, and Litao Yang. "A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice." Communications Biology 5, no. 1 (July 5, 2022). http://dx.doi.org/10.1038/s42003-022-03608-1.

Full text

Abstract:

AbstractEfficient, accurate molecular characterization of genetically modified (GM) organisms is challenging, especially for those transgenic events transferred with genes/elements of recipient species. Herein, we decipher the comprehensive molecular characterization of one novel GM rice event G281 which was transferred with native promoters and an RNA interference (RNAi) expression cassette using paired-end whole genome sequencing (PE-WGS) and modified TranSeq approach. Our results show that transgenes integrate at rice chromosome 3 locus 16,439,674 included a 36 bp deletion of rice genomic DNA, and the whole integration contains two copies of the complete transfer DNA (T-DNA) in a head-to-head arrangement. No unintended insertion or backbone sequence of the transformed plasmid is observed at the whole genome level. Molecular characterization of the G281 event will assist risk assessment and application for a commercial license. In addition, we speculate that our approach could be further used for identifying the transgene integration of cisgenesis/intragenesis crops since both ends of T-DNA in G281 rice were from native gene or elements which is similar with that of cisgenesis/intrasgenesis. Our results from the in silico mimicking cisgenesis event confirm that the mimic rice Gt1 gene insertion and its flanking sequences are successfully identified, demonstrating the applicability of PE-WGS for molecular characterization of cisgenesis/intragenesis crops.

APA, Harvard, Vancouver, ISO, and other styles

42

Mullins, Ewen, Jean‐Louis Bresson, Tamas Dalmay, Ian Crawford Dewhurst, Michelle M. Epstein, Leslie George Firbank, Philippe Guerche, et al. "Updated scientific opinion on plants developed through cisgenesis and intragenesis." EFSA Journal 20, no. 10 (October 2022). http://dx.doi.org/10.2903/j.efsa.2022.7621.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Hou, Hongwei, Neslihan Atlihan, and Zhen-Xiang Lu. "New biotechnology enhances the application of cisgenesis in plant breeding." Frontiers in Plant Science 5 (August 11, 2014). http://dx.doi.org/10.3389/fpls.2014.00389.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Obukosia, Silas, Olalekan Akinbo, Woldeyesus Sinebo, Moussa Savadogo, Samuel Timpo, Modupe Adeyemo, Sunday Akile, et al. "Update of Regulatory Options of New Breeding Techniques and Biosafety Approaches among Selected Countries: A Review." Asian Journal of Biotechnology and Bioresource Technology, November 17, 2020, 18–35. http://dx.doi.org/10.9734/ajb2t/2020/v6i330083.

Full text

Abstract:

A new set of breeding techniques, referred to as New Breeding Techniques developed in the last two decades have potential for enhancing improved productivity in crop and animal breeding globally. These include site directed nucleases based genomic editing procedures-CRISPR and Cas associated proteins, Zinc Finger Nucleases, Meganucleases/Homing Endonucleases and Transcription- Activator Like-Effector Nucleases for genome editing and other technologies including- Oligonucleotide-Directed Mutagenesis, Cisgenesis and intragenesis, RNA-Dependent DNA methylation; Transgrafting, Agroinfiltration, Reverse breeding. There are ongoing global debates on whether the processes of and products emerging from these technologies should be regulated as genetically modified organisms or approved as conventional products. Decisions on whether to regulate as GMOs are based both on understanding of the molecular basis of their development and if the GMO intermediate step was used. For example- cisgenesis, can be developed using Agrobacterium tumefaciens methods of transformation, a process used by GMO but if the selection is properly conducted the intermediate GMO elements will be eliminated and the final product will be identical to the conventionally developed crops. Others like Site Directed Nuclease 3 are regulated as GMOs in countries such as United State of America, Canada, European Union, Argentina, Australia. Progress in genome editing research, testing of genome edited bacterial blight resistant rice, development of Guidelines for regulating new breeding techniques or genome editing in Africa is also covered with special reference to South Africa, Kenya and Nigeria. Science- and evidence-based approach to regulation of new breeding techniques among regulators and policy makers should be strongly supported.

APA, Harvard, Vancouver, ISO, and other styles

45

Jacobsen, E. "Cisgenesis: a modern way of domesticating traits of the breeders' gene pool." CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 8, no. 056 (January 1, 2013). http://dx.doi.org/10.1079/pavsnnr20138056.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Mullins, Ewen, Jean‐Louis Bresson, Tamas Dalmay, Ian Crawford Dewhurst, Michelle M. Epstein, Leslie George Firbank, Philippe Guerche, et al. "Criteria for risk assessment of plants produced by targeted mutagenesis, cisgenesis and intragenesis." EFSA Journal 20, no. 10 (October 2022). http://dx.doi.org/10.2903/j.efsa.2022.7618.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

"Scientific opinion addressing the safety assessment of plants developed through cisgenesis and intragenesis." EFSA Journal 10, no. 2 (February 2012): 2561. http://dx.doi.org/10.2903/j.efsa.2012.2561.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Schicharin, Luc. "Buck Angel & les Public Service Announcement : la culture de soi sur Youtube comme stratégie de visibilité pour la communauté trans'." Recherches en Communication 36 (June 5, 2012). http://dx.doi.org/10.14428/rec.v36i36.51063.

Full text

Abstract:

Cet article interroge la mise en visibilité que la plateforme de partage de vidéos YouTube offre aux usagers trans’. Partant du constat que l’expression de soi accompagne l’émancipation des normes hégémoniques de genre, l’auteur montre comment les pratiques de cette catégorie d’usagers permettent de questionner les principes de la performativité du (trans)genre. Sur YouTube, l’autoreprésentation transgenre est confrontée aux normes corporelles cisnormatives qui exposent les trans’ à la censure. Chez les trans’, la visibilité demeure un « dilemme » entre une volonté d’assimilation et l'affirmation politique d'une identité minoritaire. Enfin, la plateforme YouTube est un outil de dénonciation et de résistances aux institutions et organisations médicales focalisées sur les corps cisgenres.

APA, Harvard, Vancouver, ISO, and other styles

49

Holme, Inger Baeksted, Claus Krogh Madsen, Toni Wendt, and Henrik Brinch-Pedersen. "Horizontal Stacking of PAPhy_a Cisgenes in Barley Is a Potent Strategy for Increasing Mature Grain Phytase Activity." Frontiers in Plant Science 11 (October 23, 2020). http://dx.doi.org/10.3389/fpls.2020.592139.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Pirrello, Carlotta, Gabriele Magon, Fabio Palumbo, Silvia Farinati, Margherita Lucchin, Gianni Barcaccia, and Alessandro Vannozzi. "Past, present and future of genetic strategies to control tolerance to the main fungal and oomycete pathogens in grapevine." Journal of Experimental Botany, December 15, 2022. http://dx.doi.org/10.1093/jxb/erac487.

Full text

Abstract:

Abstract The production of high-quality wines is strictly related to the correct management of the vineyard, which guarantees good yields and grapes with the right characteristics required for subsequent vinification. Winegrowers face a variety of challenges during the grapevine cultivation cycle: the most notorious are fungal and oomycete diseases such as downy mildew, powdery mildew, and gray mold. If not properly addressed, these diseases can irremediably compromise the harvest, with disastrous consequences for the production and wine economy. Conventional defense methods used in the past involved the use of chemical pesticides. However, such approaches are in conflict with the growing attention on environmental sustainability and shifts from the uncontrolled use of chemicals to the use of integrated approaches for crop protection. Improvements in genetic knowledge and the availability of novel biotechnologies have created new scenarios for possibly producing grapes with a reduced, if not almost zero, impact. Here, the main approaches used to protect grapevines from fungal and oomycete diseases are reviewed, starting from conventional breeding, which allowed the establishment of new resistant varieties, followed by biotechnological methods, such as transgenesis, cisgenesis, intragenesis and genome editing, and ending with more recent perspectives concerning the application of new products based on RNA interference (RNAi) technology. Evidence of their effectiveness, as well as potential risks and limitations based on the current legislative situation, are critically discussed.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Cisgenesi'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles