Relevant bibliographies by topics / Chromosome mapping

Academic literature on the topic 'Chromosome mapping'

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Published: 4 June 2021
Last updated: 31 July 2024

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Journal articles on the topic "Chromosome mapping"

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Iannucci, Alessio, Marie Altmanová, Claudio Ciofi, Malcolm Ferguson-Smith, Jorge C. Pereira, Ivan Rehák, Roscoe Stanyon, et al. "Isolating Chromosomes of the Komodo Dragon: New Tools for Comparative Mapping and Sequence Assembly." Cytogenetic and Genome Research 157, no. 1-2 (2019): 123–31. http://dx.doi.org/10.1159/000496171.

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We developed new tools to build a high-quality chromosomal map of the Komodo dragon (Varanus komodoensis) available for cross-species phylogenomic analyses. First, we isolated chromosomes by flow sorting and determined the chromosome content of each flow karyotype peak by FISH. We then isolated additional Komodo dragon chromosomes by microdissection and amplified chromosome-specific DNA pools. The chromosome-specific DNA pools can be sequenced, assembled, and mapped by next-generation sequencing technology. The chromosome-specific paint probes can be used to investigate karyotype evolution through cross-species chromosome painting. Overall, the set of chromosome-specific DNA pools of V. komodoensis provides new tools for detailed phylogenomic analyses of Varanidae and squamates in general.
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Uno, Yoshinobu, Chizuko Nishida, Chiyo Takagi, Takeshi Igawa, Naoto Ueno, Masayuki Sumida, and Yoichi Matsuda. "Extraordinary Diversity in the Origins of Sex Chromosomes in Anurans Inferred from Comparative Gene Mapping." Cytogenetic and Genome Research 145, no. 3-4 (2015): 218–29. http://dx.doi.org/10.1159/000431211.

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Sex determination in frogs (anurans) is genetic and includes both male and female heterogamety. However, the origins of the sex chromosomes and their differentiation processes are poorly known. To investigate diversity in the origins of anuran sex chromosomes, we compared the chromosomal locations of sex-linked genes in 4 species: the African clawed frog (Xenopus laevis), the Western clawed frog (Silurana/X. tropicalis), the Japanese bell-ring frog (Buergeria buergeri), and the Japanese wrinkled frog (Rana rugosa). Comparative mapping data revealed that the sex chromosomes of X. laevis, X. tropicalis and R. rugosa are different chromosome pairs; however, the sex chromosomes of X. tropicalis and B. buergeri are homologous, although this may represent distinct evolutionary origins. We also examined the status of sex chromosomal differentiation in B. buergeri, which possesses heteromorphic ZW sex chromosomes, using comparative genomic hybridization and chromosome painting with DNA probes from the microdissected W chromosome. At least 3 rearrangement events have occurred in the proto-W chromosome: deletion of the nucleolus organizer region and a paracentric inversion followed by amplification of non-W-specific repetitive sequences.
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González-Siso, M. Isabel, M. Angeles Freire-Picos, M. Esperanza Cerdán, M. Wésolowski-Louvel, and H. Fukuhara. "Chromosomal mapping of the KlCYC1 gene from Kluyveromyces lactis." Genome 37, no. 3 (June 1, 1994): 515–17. http://dx.doi.org/10.1139/g94-073.

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Chromosomal assignment of the KlCYC1 gene from Kluyveromyces lactis has been performed by hybridization of the labelled probe to a DNA blot of isolated chromosomes. A clear hybridization signal to chromosome VI is reported.Key words: Kluyveromyces lactis, cytochrome c gene, chromosomal mapping.
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Schild, David, and Robert K. Mortimer. "A MAPPING METHOD FOR SACCHAROMYCES CEREVISIAE USING rad52-INDUCED CHROMOSOME LOSS." Genetics 110, no. 4 (August 1, 1985): 569–89. http://dx.doi.org/10.1093/genetics/110.4.569.

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ABSTRACT Saccharomyces cerevisiae diploids homozygous for the rad52-1 mutation have previously been shown to lose chromosomes mitotically. Spontaneous events and events following low levels of X-ray or methyl methanesulfonate treatment result in monosomic diploids, whereas higher levels of treatment result in near haploidization. This rad52-1-dependent chromosome loss has been used to develop a new mapping method which can be used to assign a previously unmapped gene to a chromosome. Chromosome loss mapping can be done in either of two ways: (1) if a diploid, homozygous for rad52-1 but heterozygous for a variety of other recessive markers, is constructed with an unmapped recessive mutation in coupling with known chromosomal markers, chromosome loss will result in the coordinate expression of the mutation and other recessive markers on the same chromosome; (2) if, however, the diploid is constructed with the unmapped mutation in repulsion to chromosomal markers, then even haploidization will never result in the coordinate expression of the unmapped mutation and other markers on the same homologous chromosome pair—This mapping method and subsequent tetrad analyses have been used to locate hom6 on chromosome X, ade4 on chromosome XIII and cdc31 on chromosome XV and to demonstrate that met5, previously assigned to chromosome V, actually maps to chromosome X; the met- marker on chromosome V has been shown to be met6. GAL80 and SUP5, previously assigned to an unmapped fragment, have now been mapped to the right arm of chromosome XIII.
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Hausmann, Michael, C. Paul Popescu, Jeannine Boscher, Dominique Kerboœf, Jürgen Dölle, and Christoph Cremer. "Identification and Cytogenetic Analysis of an Abnormal Pig Chromosome for Flow Cytometry and Sorting." Zeitschrift für Naturforschung C 48, no. 7-8 (August 1, 1993): 645–53. http://dx.doi.org/10.1515/znc-1993-7-819.

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Abstract For cytogenetics of pig (Sus scrofa domestica) and the influence of chromosome aberrations on pig production, high interest exists in flow sorted chromosomes for gene mapping, to estab­lish DNA-libraries, or to produce DNA-probes. Flow karyotyping and sorting as well as slit scan flow analysis of metaphase chromosomes of an abnormal cell type carrying a translocation marker chromosome 6/15 are described. Flow sorting of the largest chromosomes of these cells was performed. After sorting the chromosomes still had a well preserved morphology and were identified microscopically by G-banding. The quality of the band pattern of the sorted chromosomes was compatible to that of isolated chromosomes not subjected to flow cytometry. The sorted fraction showed an enrichment of chromosom e 6/15 and chromosome 1 which have quantitatively about the same integrated fluorescence intensity. Slit scan flow analysis was performed to discriminate these two chromosomes. Metacentric and submetacentric chromosom es were analyzed according to their bimodal slit scan profiles. Profiles of the largest chromosomes were distinguished by their different centromeric indices. Two groups were interpreted as the normal chromosome 1 and the translocation chromosom e 6/15.
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Favarato, Ramon M., Leila Braga Ribeiro, Rafaela P. Ota, Celeste M. Nakayama, and Eliana Feldberg. "Cytogenetic Characterization of Two Metynnis Species (Characiformes, Serrasalmidae) Reveals B Chromosomes Restricted to the Females." Cytogenetic and Genome Research 158, no. 1 (2019): 38–45. http://dx.doi.org/10.1159/000499954.

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Karyotypes and chromosomal characteristics with focus on B chromosomes of 2 species of the serrasalmid genus Metynnis, namely M. lippincottianus and M. maculatus, were examined using conventional (C-banding) and molecular (FISH mapping of minor and major rDNAs and Rex1, Rex3, and Rex6 retrotransposable elements) protocols. Both species possessed a diploid chromosome number of 2n = 62 and karyotypes composed of 32 metacentric + 28 submetacentric + 2 subtelocentric and 32 metacentric + 26 submetacentric + 4 subtelocentric, respectively; one small B element was found in the female genome of M. lippincottianus. C-banding revealed heterochromatin in the pericentromeric and terminal portions of all chromosomes of both species; the B chromosome was entirely heterochromatic. FISH showed 18S rDNA sites in 2 chromosome pairs in both species (pairs 19 and 22), and a large block in the B chromosome, while 5S rDNA signals were detected in the first pair of subtelocentric chromosomes in both species, moreover in M. maculatus an additional labeled pair 4 was observed. Mapping of the Rex1, Rex3, and Rex6 retrotransposable elements in the genomes of M. lippincottianus and M. maculatus indicated that they were dispersed throughout nearly all the chromosomes of the complement, except for the B chromosome of M. lippincottianus.
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Goodfellow, P. N. "Mapping the Y chromosome." Development 101, Supplement (March 1, 1987): 39. http://dx.doi.org/10.1242/dev.101.supplement.39.

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DNA probes isolated from the human Y chromosome have been used to resolve two fundamental problems concerning the biology of sex determination in man. Coincidentally, resolution of these problems has generated genetic maps of the short arm of the human Y chromosome and has allowed the regional localization of TDF. The first problem to be solved was the origin of XX males (de la Chapelle, this symposium): the majority of XX males are caused by a telomeric exchange between the X and Y chromosomes that results in TDF and a variable amount of Y-derived material being transferred to the X chromosome. The differing amounts of Y-derived material present in XX males has been used as the basis of a ‘deletion’ map of the Y chromosome (Müller; Ferguson-Smith & Affara; this symposium).
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Lohe, A. R., A. J. Hilliker, and P. A. Roberts. "Mapping simple repeated DNA sequences in heterochromatin of Drosophila melanogaster." Genetics 134, no. 4 (August 1, 1993): 1149–74. http://dx.doi.org/10.1093/genetics/134.4.1149.

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Abstract Heterochromatin in Drosophila has unusual genetic, cytological and molecular properties. Highly repeated DNA sequences (satellites) are the principal component of heterochromatin. Using probes from cloned satellites, we have constructed a chromosome map of 10 highly repeated, simple DNA sequences in heterochromatin of mitotic chromosomes of Drosophila melanogaster. Despite extensive sequence homology among some satellites, chromosomal locations could be distinguished by stringent in situ hybridizations for each satellite. Only two of the localizations previously determined using gradient-purified bulk satellite probes are correct. Eight new satellite localizations are presented, providing a megabase-level chromosome map of one-quarter of the genome. Five major satellites each exhibit a multi-chromosome distribution, and five minor satellites hybridize to single sites on the Y chromosome. Satellites closely related in sequence are often located near one another on the same chromosome. About 80% of Y chromosome DNA is composed of nine simple repeated sequences, in particular (AAGAC)n (8 Mb), (AAGAG)n (7 Mb) and (AATAT)n (6 Mb). Similarly, more than 70% of the DNA in chromosome 2 heterochromatin is composed of five simple repeated sequences. We have also generated a high resolution map of satellites in chromosome 2 heterochromatin, using a series of translocation chromosomes whose breakpoints in heterochromatin were ordered by N-banding. Finally, staining and banding patterns of heterochromatic regions are correlated with the locations of specific repeated DNA sequences. The basis for the cytochemical heterogeneity in banding appears to depend exclusively on the different satellite DNAs present in heterochromatin.
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Yuan, Xiuyun, Siqi Yuan, Ya Liu, Yun Xia, and Xiaomao Zeng. "Microsatellites mapping for non-model species with chromosomal rearrangement: a case study in the frog Quasipaa boulengeri (Anura: Dicroglossidae)." Genome 60, no. 8 (August 2017): 707–11. http://dx.doi.org/10.1139/gen-2016-0200.

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Gene mapping is an important resource for understanding the evolution of genes and cytogenetics. Model species with a known genetic map or genome sequence allow for the selection of genetic markers on a desired chromosome, while it is hard to locate these markers on chromosomes of non-model species without such references. A frog species, Quasipaa boulengeri, shows chromosomal rearrangement polymorphisms, making itself a fascinating model for chromosomal speciation mediated by suppressed recombination. However, no markers have been located on its rearranged chromosomes. We present a complete protocol to map microsatellites based on mechanical microdissection and chromosome amplification techniques. Following this protocol, we mapped 71 microsatellites of Q. boulengeri at the chromosome level. In total, eight loci were assigned to rearranged chromosomes, and the other 63 loci might attach to other chromosomes. These microsatellites could be used to compare the gene flow and verify the chromosomal suppressed recombination hypothesis in Q. boulengeri. This integrated protocol could be effectively used to map genes to chromosomes for non-model species.
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Liang, Jiangtao, Simon M. Bondarenko, Igor V. Sharakhov, and Maria V. Sharakhova. "Obtaining Polytene, Meiotic, and Mitotic Chromosomes from Mosquitoes for Cytogenetic Analysis." Cold Spring Harbor Protocols 2022, no. 12 (August 5, 2022): pdb.prot107872. http://dx.doi.org/10.1101/pdb.prot107872.

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Chromosome visualization is a key step for developing cytogenetic maps and idiograms, analyzing inversion polymorphisms, and identifying mosquito species. Three types of chromosomes—polytene, mitotic, and meiotic—are used in cytogenetic studies of mosquitoes. Here, we describe a detailed method for obtaining high-quality polytene chromosome preparations from the salivary glands of larvae and the ovaries of females forAnophelesmosquitoes. We also describe how to obtain mitotic chromosomes from imaginal discs of fourth-instar larvae and meiotic chromosomes from the testes of male pupae for all mosquitoes. These chromosomes can be used for fluorescence in situ hybridization (FISH), a fundamental technique in cytogenetic research that is used for physical genome mapping, detecting chromosomal rearrangements, and studying chromosome organization.
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Dissertations / Theses on the topic "Chromosome mapping"

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Morroll, Shaun Michael. "Mapping of yeast artificial chromosomes from Arabidopsis chromosome 5." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308922.

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Brinkman-Mills, Polly. "Transcriptional mapping in human chromosome 22q11.2." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0015/MQ47011.pdf.

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Apostolou, Sinoula. "Physical mapping of human chromosome 16." Title page, contents and abstract only, 1997. http://web4.library.adelaide.edu.au/theses/09PH/09pha645.pdf.

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Dutton, Elizabeth R. "Mapping studies on mouse distal Chromosome 2." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299401.

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Creavin, Treasa Agnes Della Geraldine. "Transcriptional mapping of human chromosome 16p12.3-p12.2." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321891.

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Mazaheri, Mona. "Radiation Hybrid Mapping of Barley Chromosome 3H." Diss., North Dakota State University, 2014. https://hdl.handle.net/10365/27454.

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Assembly of the barley (Hordeum vulgare L.) genome requires high resolution maps for aligning contig-based physical maps along chromosomes. Genetic maps lack accurate information on the physical position of almost half of the barley genome located in recombination-poor regions. Radiation hybrid (RH) mapping is an alternative approach, which is based on radiation-induced chromosomal deletions. In this study, an RH population for barley chromosome 3H was developed. Genotyping 373 3H-RH lines with 113 markers resulted in an RH map with an average resolution of 2.22 Kb. Compared to an analogous genetic map, the 3H-RH map resolution was 9.53-X higher, reaching to >262.40-X better resolution in the centromeric region. We suggest that RH maps would facilitate assembly of the barley genome. For future RH studies of the barley genome, an optimum genotyping platform, consisting of 400,536 barley-specific repeat junction markers (RJMs), was developed.
Frank Bain Dissertation Fellowship
Charles and Linda Moses Presidential Graduate Fellowship
North Dakota State University. College of Agriculture, Food Systems and Natural Resources
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He, Bing. "Susceptibility gene mapping in multiple sclerosis /." Stockholm, 1998. http://diss.kib.ki.se/search/diss.se.cfm?19980608he.

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Söderhäll, Cilla. "Gene mapping of atopic dermatitis /." Stockholm : [Karolinska institutets bibl.], 2001. http://diss.kib.ki.se/2001/91-7349-088-1/.

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Stephens, Sarah H. "Fine mapping of the chromosome 15q13-14 schizophrenia linkage region /." Connect to full text via ProQuest. Limited to UCD Anschutz Medical Campus, 2008.

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Thesis (Ph.D. in Human Medical Genetics) -- University of Colorado Denver, 2008.
Typescript. Includes bibliographical references (leaves 112-128). Free to UCD Anschutz Medical Campus. Online version available via ProQuest Digital Dissertations;
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Hinkley, Craig S. (Craig Steven). "Gene Dosage Study on Human Chromosome 22." Thesis, North Texas State University, 1986. https://digital.library.unt.edu/ark:/67531/metadc500617/.

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A gene dosage study was conducted on a rare complete trisomy 22 human fibroblast cell line utilizing three lysosomal enzymes, ∝-iduronidase, ∝-galactosidase B, and arylsulfatase A, whose genes are located on chromosome 22 and two control enzymes, ,β-hexosaminidase A and -- fucosidase, with genes not on chromosome 22. A gene dosage effect was clearly demonstrated for an early passage number of the fibroblasts; however, later passage numbers gave inconclusive results. This study suggests that gene dosage studies must be carefully designed to be conducted only on early, matched passage number cells. ∝-fucosidase gave anomalous results most likely due to pleiotropic effects. The present gene dosage study confirmed the trisomic nature of the cell line studied and suggests that this type of study may be a useful diagnostic tool for small deletions, additions, or unbalanced translocations.
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Books on the topic "Chromosome mapping"

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F, Verrity Jennifer, and Abbington Lilian E, eds. Chromosome mapping research developments. New York: Nova Science, 2008.

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James, Louise Anne. Physical mapping on human chromosome 3 using yeast artificial chromosomes. Manchester: University of Manchester, 1994.

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B, Schook Lawrence, Lewin Harris Alan, and McLaren David G, eds. Gene-mapping techniques and applications. New York: Marcel Dekker, 1991.

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Scherer, Stephen W. Physical mapping of human chromosome 7 with yeast artificial chromosome (YAC) vectors. Ottawa: National Library of Canada = Bibliothèque nationale du Canada, 1991.

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United States. Congress. Senate. Committee on Energy and Natural Resources. Subcommittee on Energy Research and Development. The human genome project: Hearing before the Subcommittee on Energy Research and Development of the Committee on Energy and Natural Resources, United States Senate, One Hundred First Congress, second session on the human genome project, July 11, 1990. Washington: U.S. G.P.O., 1990.

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United States. Department of Energy. Health and Environmental Research Advisory Committee. Subcommittee on Human Genome. Report on the human genome initiative for the Office of Health and Environmental Research. Washington, DC]: [D.O.E.], 1987.

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Jacqueline, Boultwood, ed. Gene isolation and mapping protocols. Totowa, N.J: Humana Press, 1997.

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Leong, Merlin Mei Lun. Current gene mapping methods. Leioa-Vizcaya, Spain]: Servicio Editorial Universidad de Pais Vasco, 1985.

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E, Davies K., ed. Genome analysis: A practical approach. Oxford: IRL Press, 1988.

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Poptsova, Maria S. Genome analysis: Current procedures and applications. Norfolk, UK: Caister Academic Press, 2014.

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Book chapters on the topic "Chromosome mapping"

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Clark, M. S., and W. J. Wall. "Chromosome mapping." In Chromosomes, 176–205. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-0073-8_7.

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Gooch, Jan W. "Chromosome Mapping." In Encyclopedic Dictionary of Polymers, 882. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13392.

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Appels, Rudi, Rosalind Morris, Bikram S. Gill, and Cedric E. May. "Genetic and Molecular Mapping of Chromosomes." In Chromosome Biology, 318–37. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-5409-7_21.

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Keshava, Rohini. "Chromosome Mapping in Eukaryotes." In Genetics Fundamentals Notes, 165–237. Singapore: Springer Nature Singapore, 2022. http://dx.doi.org/10.1007/978-981-16-7041-1_4.

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Malembic-Maher, Sylvie, and Patricia Carle. "Mapping the Phytoplasma Chromosome." In Methods in Molecular Biology, 405–16. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-089-2_34.

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Gill, Bikram S. "A Century of Cytogenetic and Genome Analysis: Impact on Wheat Crop Improvement." In Wheat Improvement, 277–97. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-90673-3_16.

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AbstractBeginning in the first decade of 1900, pioneering research in disease resistance and seed color inheritance established the scientific basis of Mendelian inheritance in wheat breeding. A series of breakthroughs in chromosome and genome analysis beginning in the 1920s and continuing into the twenty-first century have impacted wheat improvement. The application of meiotic chromosome pairing in the 1920s and plasmon analysis in the 1950s elucidated phylogeny of the Triticum-Aegilops complex of species and defined the wheat gene pools. The aneuploid stocks in the 1950s opened floodgates for chromosome and arm mapping of first phenotypic and later protein and DNA probes. The aneuploid stocks, coupled with advances in chromosome banding and in situ hybridization in the 1970s, allowed precise chromosome engineering of traits in wide hybrids. The deletion stocks in the 1990s were pivotal in mapping expressed genes to specific chromosome bins revealing structural and functional differentiation of chromosomes along their length and facilitating map-based cloning of genes. Advances in whole-genome sequencing, chromosome genomics, RH mapping and functional tools led to the assembly of reference sequence of Chinese Spring and multiple wheat genomes. Chromosome and genomic analysis must be integrated into wheat breeding and wide-hybridizaton pipeline for sustainable crop improvement.
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Tanksley, Steven D., Joyce Miller, Andrew Paterson, and Robert Bernatzky. "Molecular Mapping of Plant Chromosomes." In Chromosome Structure and Function, 157–73. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-1037-2_7.

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Šimková, Hana, Petr Cápal, and Jaroslav Doležel. "Wheat Chromosomal Resources and Their Role in Wheat Research." In Compendium of Plant Genomes, 27–50. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-38294-9_3.

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AbstractBread wheat (Triticum aestivum L.) is grown on more area of land than any other crop, and its global significance is challenged only by rice. Despite the socioeconomic importance, the wheat genome research was lagging behind other crops for a long time. It was mainly a high complexity of the genome, polyploidy and a high content of repetitive elements that were laying obstacles to a thorough genome analysis, gene cloning and genome sequencing. Solution to these problems came in the beginning of the new millennium with the emergence of chromosome genomics—a new approach to studying complex genomes after dissecting them into smaller parts—single chromosomes or their arms. This lossless complexity reduction, enabled by flow-cytometric chromosome sorting, reduced the time and cost of the experiment and simplified downstream analyses. Since the approach overcomes difficulties due to sequence redundancy and the presence of homoeologous subgenomes, the chromosomal genomics was adopted by the International Wheat Genome Sequencing Consortium (IWGSC) as the major strategy to sequence bread wheat genome. The dissection of the wheat genome into single chromosomes enabled the generation of chromosome survey sequences and stimulated international collaboration on producing a reference-quality assembly by the clone-by-clone approach. In parallel, the chromosomal resources were used for marker development, targeted mapping and gene cloning. The most comprehensive approaches to gene cloning, such as MutChromSeq and assembly via long-range linkage, found their use even in the post-sequencing era. The chapter provides a two-decade retrospective of chromosome genomics applied in bread wheat and its relatives and reports on the chromosomal resources generated and their applications.
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Li, Le, Trude Schwarzacher, Paulina Tomaszewska, Qing Liu, Xiaoyu Zoe Li, Kexian Yi, Weihuai Wu, and J. S. Pat Heslop-Harrison. "Protocols for Chromosome Preparations: Molecular Cytogenetics and Studying Genome Organization in Coffee." In Mutation Breeding in Coffee with Special Reference to Leaf Rust, 291–314. Berlin, Heidelberg: Springer Berlin Heidelberg, 2023. http://dx.doi.org/10.1007/978-3-662-67273-0_21.

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AbstractCytological preparations from cell nuclei are required to count the number of chromosomes (including determining ploidy or aneuploidy), to investigate their morphology and organization. The results are valuable for genetic and evolutionary studies, and in breeding programs to understand species relationships, polyploidy, and potential introgression of chromosomes in hybrids between different species. Preparation of good chromosome spreads with well-separated metaphase chromosomes is the foundation of cytogenetic research including chromosomal mapping based on FISH (fluorescence in situ hybridization). FISH combined with specific locus probes correlated with molecular markers to specific chromosomes for integrating physical and linkage maps as well as studying the genetic evolution of allopolyploidization, has rarely been applied in Coffea spp. despite being a global high-value crop. Cytogenetic studies of Coffea are limited by the small size and similar morphology of the chromosomes, but FISH can help to map sequences to chromosome arms and identify individual chromosomes. This chapter presents protocols for germinating seeds and growing coffee plants involving pre-treatment and fixation of root-tips where the meristems of actively growing roots have many divisions. Mitotic metaphase chromosome preparation on microscope slides is described, as well as preparing probes of 5S and 18S rDNA to be used for FISH. The FISH experiments involve a two-step protocol with pre-treatments and setting up the hybridization on day 1 and the detection of probe sites on day 2 after overnight hybridization. A final section gives advice about visualization using a fluorescent microscope and capturing images.
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Gisselsson, David. "Refined characterisation of chromosome aberrations in tumours by multicolour banding and electronic mapping resources." In Chromosome Painting, 23–28. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0330-8_4.

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Conference papers on the topic "Chromosome mapping"

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Liu, Yu, Kaitai Zhang, Xinyu Zhang, Yanning Gao, and Ying Hu. "ArraySVG: Chromosome mapping and network visualization of multi-platform data." In 2010 3rd International Conference on Biomedical Engineering and Informatics (BMEI). IEEE, 2010. http://dx.doi.org/10.1109/bmei.2010.5639343.

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Sharakhova, Maria V. "Advanced cytogenetic physical mapping for the development of chromosome-based genome assemblies." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.109079.

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Jain, Sakait, and Hae Chang Gea. "Two-Dimensional Packing Problems Using Genetic Algorithms." In ASME 1996 Design Engineering Technical Conferences and Computers in Engineering Conference. American Society of Mechanical Engineers, 1996. http://dx.doi.org/10.1115/96-detc/dac-1466.

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Abstract This paper presents a technique for applying genetic algorithms for the two dimensional packing problem. The approach is applicable to not only convex shaped objects, but, can also accommodate any type of concave and complex shaped objects including objects with holes. In this approach, a new concept of a two dimensional genetic chromosome is introduced. The total layout space is divided into a finite number of cells for mapping it into this 2-D genetic algorithm chromosome. The mutation and crossover operators have been modified and are applied in conjunction with connectivity analysis for the objects to reduce the creation of faulty generations. A new feature has been added to the genetic algorithm(GA) in the form of a new operator called compaction. Several examples of GA based layout are presented.
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"373. Fine-mapping young-stock survival QTL on chromosome 6 in Nordic Red Dairy Cattle." In World Congress on Genetics Applied to Livestock Production. The Netherlands: Wageningen Academic Publishers, 2022. http://dx.doi.org/10.3920/978-90-8686-940-4_373.

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Feusier, Julie Ellen, Michael J. Madsen, Karl Voelkerding, Martha Glenn, and Nicola Camp. "Abstract 2119: Gene mapping in high-risk chronic lymphocytic leukemia pedigree identifies risk locus at chromosome 2q22.1." In Proceedings: AACR Annual Meeting 2020; April 27-28, 2020 and June 22-24, 2020; Philadelphia, PA. American Association for Cancer Research, 2020. http://dx.doi.org/10.1158/1538-7445.am2020-2119.

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Mills, Alea A. "Abstract IA-1: From chromosome engineering to chromatin remodeler: CHD5 is a tumor suppressor mapping to human 1p36." In Abstracts: First AACR International Conference on Frontiers in Basic Cancer Research--Oct 8–11, 2009; Boston MA. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.fbcr09-ia-1.

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Parikh, Hemang M., Zhaoming Wang, Kerry Pettigrew, Jinping Jia, Sarah Daugherty, Meredith Yeager, Kevin Jacobs, et al. "Abstract 2778: Fine mapping the KLK3 locus on chromosome 19q13.33 associated with prostate cancer susceptibility and PSA levels." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-2778.

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Ruterbusch, Julie J., Albert M. Levin, Rick Kittles, Benjamin A. Rybicki, and Cathryn H. Bock. "Abstract A66: Admixture and fine mapping in African Americans identifies a susceptibility locus for prostate cancer on chromosome 7." In Abstracts: Fifth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; Oct 27–30, 2012; San Diego, CA. American Association for Cancer Research, 2012. http://dx.doi.org/10.1158/1055-9965.disp12-a66.

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Jain, Sakait, and Hae Chang Gea. "PCB Layout Design Using a Genetic Algorithm." In ASME 1995 Design Engineering Technical Conferences collocated with the ASME 1995 15th International Computers in Engineering Conference and the ASME 1995 9th Annual Engineering Database Symposium. American Society of Mechanical Engineers, 1995. http://dx.doi.org/10.1115/detc1995-0070.

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Abstract This paper presents an approach to find the optimal design layout of chips on a circuit board in a manner that minimizes the area covered on the board and the connections between the various chips. In addition, there are no major heat sources next to each other and certain physical constraints are satisfied while finding a layout design. In this approach, the whole circuit board area is divided into a finite number of cells for mapping it into a genetic algorithm chromosome. The mutation and crossover operators have been modified and are applied in conjunction with connectivity analysis for the chips to reduce the creation of a lot of faulty generations. Examples of GA based chip layout are presented to show how each of the objectives are attained separately followed by examples to arrive at layouts using multiple objectives.
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Williams, Julia L., Maisa Yoshimoto, Olga Ludkovski, Sircar Kanishka, Tarek Bismar, Andrew Evans, and Jeremy A. Squire. "Abstract 320: Fine structure genomic mapping of chromosome 10q23 interstitial deletions in prostate cancer reveals thePTENgene locus as the minimum region deleted." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-320.

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Reports on the topic "Chromosome mapping"

1

Antonarakis, S. E. Human Chromosome 21: Mapping of the chromosomes and cloning of cDNAs. Office of Scientific and Technical Information (OSTI), September 1991. http://dx.doi.org/10.2172/6397375.

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Antonarakis, S. E. Human chromosome 21: Linkage mapping and cloning in yeast artificial chromosomes. Office of Scientific and Technical Information (OSTI), January 1991. http://dx.doi.org/10.2172/6278130.

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Feldman, Moshe, Eitan Millet, Calvin O. Qualset, and Patrick E. McGuire. Mapping and Tagging by DNA Markers of Wild Emmer Alleles that Improve Quantitative Traits in Common Wheat. United States Department of Agriculture, February 2001. http://dx.doi.org/10.32747/2001.7573081.bard.

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The general goal was to identify, map, and tag, with DNA markers, segments of chromosomes of a wild species (wild emmer wheat, the progenitor of cultivated wheat) determining the number, chromosomal locations, interactions, and effects of genes that control quantitative traits when transferred to a cultivated plant (bread wheat). Slight modifications were introduced and not all objectives could be completed within the human and financial resources available, as noted with the specific objectives listed below: 1. To identify the genetic contribution of each of the available wild emmer chromosome-arm substitution lines (CASLs) in the bread wheat cultivar Bethlehem for quantitative traits, including grain yield and its components and grain protein concentration and yield, and the effect of major loci affecting the quality of end-use products. [The quality of end-use products was not analyzed.] 2. To determine the extent and nature of genetic interactions (epistatic effects) between and within homoeologous groups 1 and 7 for the chromosome arms carrying "wild" and "cultivated" alleles as expressed in grain and protein yields and other quantitative traits. [Two experiments were successful, grain protein concentration could not be measured; data are partially analyzed.] 3. To derive recombinant substitution lines (RSLs) for the chromosome arms of homoeologous groups 1 and 7 that were found previously to promote grain and protein yields of cultivated wheat. [The selection of groups 1 and 7 tons based on grain yield in pot experiments. After project began, it was decided also to derive RSLs for the available arms of homoeologous group 4 (4AS and 4BL), based on the apparent importance of chromosome group 4, based on early field trials of the CASLs.] 4. To characterize the RSLs for quantitative traits as in objective 1 and map and tag chromosome segments producing significant effects (quantitative trait loci, QTLs by RFLP markers. [Producing a large population of RSLs for each chromosome arm and mapping them proved more difficult than anticipated, low numbers of RSLs were obtained for two of the chromosome arms.] 5. To construct recombination genetic maps of chromosomes of homoeologous groups 1 and 7 and to compare them to existing maps of wheat and other cereals [Genetic maps are not complete for homoeologous groups 4 and 7.] The rationale for this project is that wild species have characteristics that would be valuable if transferred to a crop plant. We demonstrated the sequence of chromosome manipulations and genetic tests needed to confirm this potential value and enhance transfer. This research has shown that a wild tetraploid species harbors genetic variability for quantitative traits that is interactive and not simply additive when introduced into a common genetic background. Chromosomal segments from several chromosome arms improve yield and protein in wheat but their effect is presumably enhanced when combination of genes from several segments are integrated into a single genotype in order to achieve the benefits of genes from the wild species. The interaction between these genes and those in the recipient species must be accounted for. The results of this study provide a scientific basis for some of the disappointing results that have historically obtained when using wild species as donors for crop improvement and provide a strategy for further successes.
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Sutherland, G. R. Physical mapping of human chromosome 16. Office of Scientific and Technical Information (OSTI), January 1992. http://dx.doi.org/10.2172/7236268.

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Connolly, Sarah. Mapping genes to human chromosome 19. Office of Scientific and Technical Information (OSTI), May 1996. http://dx.doi.org/10.2172/576741.

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Marrone, B. L., L. L. Deaven, D. J. Chen, Min S. Park, M. A. MacInnes, G. C. Salzman, and T. M. Yoshida. Directly labeled fluorescent DNA probes for chromosome mapping. Office of Scientific and Technical Information (OSTI), December 1995. http://dx.doi.org/10.2172/205135.

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Caskey, C., D. Nelson, and D. Ledbetter. Mapping and ordered cloning of the human X chromosome. Office of Scientific and Technical Information (OSTI), October 1989. http://dx.doi.org/10.2172/5518435.

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Caskey, C. T., and D. L. Nelson. Mapping and ordered cloning of the human X chromosome. Office of Scientific and Technical Information (OSTI), December 1992. http://dx.doi.org/10.2172/6387495.

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Sutherland, G. R. Physical mapping of human chromosome 16. Annual progress report. Office of Scientific and Technical Information (OSTI), August 1992. http://dx.doi.org/10.2172/10167242.

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Buenaventura, J. M. The mapping of novel genes to human chromosome 19. Office of Scientific and Technical Information (OSTI), December 1994. http://dx.doi.org/10.2172/96636.

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