Dissertations / Theses on the topic 'Chromatographic purification'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Chromatographic purification.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Hamdi, Anis. "Novel Chromatographic methodology for virus particles purification." Master's thesis, Universidade Nova de Lisboa, Instituto de Tecnologia Química e Biológica António Xavier, 2016. http://hdl.handle.net/10362/64186.
Full textN/A
Mekaoui, Nazim. "Contribution à l'étude de la chromatographie à contre-courant : partage de composés ionisables, nouvelles colonnes et purification séquentielles." Thesis, Lyon 1, 2012. http://www.theses.fr/2012LYO10249/document.
Full textCounter-current chromatography (CCC) is a preparative purification technique that works with the twoliquid phases of a biphasic liquid system. One phase is used as the mobile phase when the other phase isused as the stationary phase. There is no solid support: centrifugal fields are used to obtain a support-freeliquid stationary phase. This work contains an exhaustive bibliographic study of what can be found in theliterature concerning continuous chromatographic processes. The multi-dual-mode (MDM) process was foundto be the best one able to purify large amount of crude mixtures. The MDM method starts with a classicalseparation of the mixture followed by a switch of both the liquid phase nature and the flowing direction. Themobile phase flowing e.g. in a descending direction becomes the stationary phase. The previous stationaryphase becomes the mobile phase flowing in the ascending direction (or vice versa). The purified compoundsof the introduced mixture are eluted at one side of the column or the other according to their polarity. TheMDM method was used to purify a crude sample of Coomassie Blue: the polar part of the dye was eluted atthe column top (or head) and the apolar part at the column bottom (or tail) while the essential part of the dyewas trapped inside the CCC column. The work also presents a new small volume (30 mL) hydrostatic CCCcolumn. It is shown that this column could be used to test quickly the potential of a given biphasic liquidsystem
Balci, Oguz. "Affinity chromatographic purification of recombinant human growth hormone." Master's thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/2/12609269/index.pdf.
Full textselected oligonucleotides were synthesized and used as ligands. Effect of pH on ligand-human growth hormone complex formation was investigated and the highest complex formation was obtained at pH= 7.0. Human growth hormone is separated from the fermentation broth with 99.8% purity and 41% overall yield. The equilibrium data obtained was described by Langmuir type isotherm where saturation constant (q0) and affinity constant (K) are calculated as 0.338 mg hGH/µ
mol aptamer and 0.059 mg hGH/ml, respectively. Further, equilibrium data obtained using aptamer affinity column was described by Langmuir type isotherm where saturation constant (q0) and affinity constant (K) are 0.027 mg hGH/µ
mol aptamer and 1.543 mg hGH/ml, respectively. It is possible that, selected aptamer can be used for purification of bulk amounts of recombinant human growth hormone by using aptamer affinity chromatography.
Ramat, Fabien M. "Protein purification using expanded bed chromatography." Link to electronic thesis, 2003. http://www.wpi.edu/Pubs/ETD/Available/etd-0114104-114704.
Full textKeywords: zirconia; protein purification; anion exchange; chicken egg white; expanded bed chromatography. Includes bibliographical references (p. 83-86).
Nakamura, Koji. "Studies on High-performance Affinity Chromatography : Preparation of the Chromatographic Gels, Evaluation of the Chromatographic Conditions, and the Application to Purification of Enzymes." Kyoto University, 2004. http://hdl.handle.net/2433/148344.
Full textBergs, Dominik [Verfasser]. "A contribution to chromatographic purification of natural products / Dominik Bergs." München : Verlag Dr. Hut, 2013. http://d-nb.info/1045989150/34.
Full textRamat, Fabien M. "Protein purification using expanded bed chromatography." Digital WPI, 2004. https://digitalcommons.wpi.edu/etd-theses/92.
Full textPate, Martin Eric. "A practical investigation into the use of principal component analysis for the modelling and scale-up of high performance liquid chromatography." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.322003.
Full textKochendörfer, Kiara [Verfasser]. "Chromatographic Purification of an Intermediately Eluting Component from a Complex Mixture / Kiara Kochendörfer." München : Verlag Dr. Hut, 2017. http://d-nb.info/1126297976/34.
Full textJin, J. "Lipid foulant interactions during the chromatographic purification of virus-like particles from Saccharomyces cerevisiae." Thesis, University College London (University of London), 2011. http://discovery.ucl.ac.uk/1302065/.
Full textMaeda, Atsushi. "Production of 3-Ketocellobiose from Cellobiose Using Agrobacterium tumefaciens Cells and Its Chromatographic Purification." Kyoto University, 2003. http://hdl.handle.net/2433/149002.
Full text0048
新制・課程博士
博士(農学)
甲第10278号
農博第1350号
新制||農||869(附属図書館)
学位論文||H15||N3799(農学部図書室)
UT51-2003-H699
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 松野 隆一, 教授 井上 國世, 教授 村田 幸作
学位規則第4条第1項該当
Ma, Chun-hang. "Partial purification and characterization of 1-aminocyclopropane-1-carboxylic acid n-malonyltransferase from etiolated mung bean." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B36181341.
Full textBonturi, Nemailla 1985. "Purificação em etapa cromatográfica única de DNA plasmidial a partir do lisado neutralizado visando a sua aplicação em estudos de terapia e vacinação gênica." [s.n.], 2011. http://repositorio.unicamp.br/jspui/handle/REPOSIP/266888.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Química
Made available in DSpace on 2018-08-18T15:34:36Z (GMT). No. of bitstreams: 1 Bonturi_Nemailla_M.pdf: 2076232 bytes, checksum: e3f4e56f400891ab77fe029e0c1f0899 (MD5) Previous issue date: 2011
Resumo: O número de estudos em terapia gênica com vetores plasmídiais (pDNA) têm aumentado nestes últimos anos. Como resultado, a demanda para preparações de pDNA em conformidade com as recomendações das agências reguladoras (EMEA, FDA) também aumentou. O DNA plasmidial é frequentemente obtido através da fermentação de Escherichia coli transformada e purificada por uma série de operações unitárias, incluindo a cromatografia. Este trabalho teve como objetivo o desenvolvimento de um processo cromatográfico para a recuperação e purificação do pDNA superenovelado (sc pDNA) a partir do lisado neutralizado. Os ligantes fenil (hidrofóbico) e mercaptopirimidina (tiofílico) foram imobilizados em matrizes de agarose e celulose. A seletividade destes ligantes para com o sc pDNA foi determinada através de estudos de adsorção utilizando citrato de sódio 1,5 mol/L e fosfato de potássio 2,0 mol/L como tampões de adsorção. A cromatografia com o adsorvente fenil-agarose e o citrato de sódio 1,5 mol/L permitiu recuperar 58% do pDNA sem contaminação por gDNA, proteínas e endotoxinas, sendo uma alternativa potencial para a recuperação primária do sc pDNA. O resultado mais promissor foi obtido com a cromatografia com o adsorvente mercaptopirimidina-agarose e fosfato de potássio 2,0 mol/L como tampão de adsorção. Este sistema tampão de adsorção/adsorvente permitiu a obtenção de pDNA com 100% de pureza e dentro das recomendações das agências reguladoras no tocante à contaminação por RNA e endotoxinas. Assim, este trabalho lançou as bases para o desenvolvimento de dois métodos cromatográficos para a recuperação primária ou purificação de pDNA diretamente do lisado neutralizado, ambos potencialmente aplicáveis em larga escala
Abstract: The number of studies in gene therapy with plasmid vectors (pDNA) has witnessed an increase in the recent years. As result the demand for preparations of pDNA in compliance with recommendations of the regulatory agencies (EMEA, FDA) has also increased. Plasmid DNA is oftenly obtained through fermentation of transformed Escherichia coli and purified by a series of unit operations, including chromatography. This work aimed the development of a chromatographic process for the recovery and purification of supercolied pDNA (sc pDNA) directly from neutralized cell lysate. Phenyl (hydrophobic) and mercaptopyrimidine (thiophilic) molecules immobilized in agarose and cellulose matrices were the ligands used to capture the pDNA. Their selectivity towards sc pDNA was evaluated through adsorption studies using sodium citrate 1.5 mol/L and potassium phosphate 2.0 mol/L as the adsorption buffers. The chromatography with the adsorbent phenyl-agarose and sodium citrate 1.5 mol/L was able to recover 58% of sc pDNA without gDNA, proteins and endotoxins contamination, being an potential alternative for the primary recovery of sc pDNA. The most promising result was obtained with the chromatography with mercaptopyrimidine-agarose and potassium phosphate 2.0 mol/L adsorpition buffer. With the latter buffer/adsorbent system it was possible to obtain in a single step pDNA with 100% purity and within the recommendations of regulatory agencies with regard to contamination by RNA and endotoxins. Thus, this work laid the basis for the development of two chromatographic process for the recovery or purification of pDNA directly from the neutralized lysate, both potentially applicable in larger scale
Mestrado
Desenvolvimento de Processos Biotecnologicos
Mestre em Engenharia Química
Kouyoumdjian, Arthur Jean Michel. "The functionalisation and application of microporous micro-capillary films for the chromatographic purification of biomolecules." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/289020.
Full textMa, Chun-hang, and 馬進恆. "Partial purification and characterization of 1-aminocyclopropane-1-carboxylic acid n-malonyltransferase from etiolated mung bean." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B36181341.
Full textChapman, James M. "The preparation and evaluation of immobilized retinoid analogues in the affinity chromatographic purification of retinoid binding proteins /." The Ohio State University, 1990. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487678444256637.
Full textKissounko, Natalia. "Study of dynamics in a reaction catalyzed by ht- and ps-ADH cloning, purification and preliminary x-ray screening /." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 80 p, 2008. http://proquest.umi.com/pqdweb?did=1605142871&sid=6&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Full textGaliardi, Jackelyn. "Split Intein Applications for Downstream Purification and Protein Conjugation." The Ohio State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1618931990774251.
Full textKröner, Frieder [Verfasser], and J. [Akademischer Betreuer] Hubbuch. "Novel approaches in chromatographic purification process development for low concentrated proteins in complex mixtures / Frieder Kröner. Betreuer: J. Hubbuch." Karlsruhe : KIT-Bibliothek, 2013. http://d-nb.info/1046362674/34.
Full textPhilander, Ghouwaa. "Development of a gas chromatographic technique for the analysis of some groundwater contaminants from fuel leaks and its application in a site-specific study." Thesis, University of the Western Cape, 2009. http://hdl.handle.net/11394/2613.
Full textThis study focuses on the development of a Direct Aqueous Injection Gas Chromatographic method with Flame Ionization Detection (DAI-GC/FID) for the analysis of MTBE and TBA. The analytical method was then applied in a site specific study where MTBE contamination was evident. The method achieved detection limits of 1 ppm for MTBE and 0.1 ppm for TBA. The method showed good precision, accuracy and selectivity. The method was selected primarily for its ability to simultaneously analyze MTBE and TBA. The result of the site specific study showed the persistence of high concentrations of MTBE and TBA at the source of contamination, whilst concentrations at the adjacent primary school dropped to below detection limits as a result of rapid natural attenuation. It was found that an overall decrease in MTBE concentrations was met with an increase in TBA concentrations; which is a direct indication of MTBE degradation. Despite the fact that problematic MTBE concentrations persist at the source of contamination, limited evidence of the persistence of MTBE contamination was identified at the adjacent primary school. As such, MTBE health risks from existing pathways were found to be irrelevant for receptors at the adjacent school.
South Africa
Butters, Terry Douglas. "Ligand-affinity chromatographic purification of α-fucosidases and α-glucosidases : tools for the structural characterisation of N-linked oligosaccharides from diverse species." Thesis, Open University, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.296614.
Full textMarichal-Gallardo, Pável Alejandro Verfasser], and Udo [Gutachter] [Reichl. "Chromatographic purification of biological macromolecules by their capture on hydrophilic surfaces with the aid of non-ionic polymers / Pável Alejandro Marichal-Gallardo ; Gutachter: Udo Reichl." Magdeburg : Universitätsbibliothek Otto-von-Guericke-Universität, 2019. http://d-nb.info/1219965022/34.
Full textMarichal-Gallardo, Pável Alejandro [Verfasser], and Udo [Gutachter] Reichl. "Chromatographic purification of biological macromolecules by their capture on hydrophilic surfaces with the aid of non-ionic polymers / Pável Alejandro Marichal-Gallardo ; Gutachter: Udo Reichl." Magdeburg : Universitätsbibliothek Otto-von-Guericke-Universität, 2019. http://nbn-resolving.de/urn:nbn:de:gbv:ma9:1-1981185920-332329.
Full textMarlot, Léa. "Développement de méthodes bidimensionnelles préparatives CPCxLC : application à la purification de molécules d'intérêt issues de matrices végétales." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1299/document.
Full textPreparative two-dimensional chromatography is gaining interest in the elucidation of complex samples as it allows the collection of a large number of molecules with high recovered purity and quantity. While the second dimension is often selected to be liquid chromatography (LC), centrifugal partition chromatography (CPC) is a technique with multiple advantages representing a suitable first dimension. In order to purify several molecules of interest in plant matrices, the comprehensive CPCxLC represents a technique with high potential. After explaining its interest and the issues related to the preparative separation in comprehensive mode, the development of such a separation is studied according to three axes. Firstly, a purification of two targeted molecules in Edelweiss plant is carried out at industrial scale thanks to the realization of 2D-contour plot. This application allows to expose the interest of the separation and to highlight the locks related to the conditions of total transfer of the fractions in second dimension. In a second part, the comprehensive CPCxLC separation is developed with the total transfer of the sample in second dimension applied to the purification of five target compounds from Edelweiss plant. The key points of the CPCxLC separation, namely the sampling time and the second dimension transfer, are studied with regard to the LCxLC separation in order to ensure a separation quality allowing the total recovery of the compounds. Finally, the third part consists in the implementation of a CPCxLC system selection methodology based on the quantitative evaluation of the potential of two-dimensional systems to generate distance between peaks. This selection procedure is developed on the sample Cyclopia genistoides with the objective of isolating eight target compounds
Bouiche, Feriel. "Amélioration instrumentale de la chromatographie de partage centrifuge en vue de la purification de molécules très polaires." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1003/document.
Full textThe aim of this thesis is to develop a new centrifugal partition chromatography (CPC) instrument in order to purify highly polar molecules. CPC is a preparative technique for the separation of molecules using a solvent system composed of two immiscible liquids. This manuscript describes the different protein purification techniques used in industrial production process. A focus is made on the use of aqueous biphasic systems for the purification of biomolecules, which represents a real trend in the industry thanks to its low cost, scaling simplicity and especially the favorable environment that it provides to biomolecules. Thus, based on the advantages of these solvent systems known as Aqueous Two Phase Systems (ATPS), CPC could provide additional performances to purify proteins at lower cost. To respond to this industrial challenge, it is necessary to develop both innovative chromatographic methods and new devoted instruments. Indeed, current CPC instruments are not compatible with Good Manufacturing Practices due to the presence of Teflon seals which prevents the possibility of sterilizing the instruments. The manufacture of a new monobloc instrument entirely made of titanium was achieved thanks to the 3D printing technology. The purpose of this thesis is the evaluation of this new column performance in order to determine its applicability to biomolecules purification. A special attention is also provided to the injection of very small sample volumes in order to facilitate method development
Blanc, Claire-Line. "Conception et optimisation d’un procédé innovant pour la purification d’acides organiques issus de biotechnologie." Thesis, Châtenay-Malabry, Ecole centrale de Paris, 2015. http://www.theses.fr/2015ECAP0008.
Full textThe objective of this study is to evaluate the use of preparative chromatography in the context of the elaboration and optimization of an innovative purification process of organic acids from biotechnology. Lactic and succinic acids were mainly studied. They are produced by fermentation and used in industry as additive, for a long time. They are identified as promising building blocks for green chemistry development, from renewable carbon. In particular, they are monomers for bioplastic industry. Unlike historical utilizations, this new type of application requires much higher purity levels. Those purities are currently obtained by additional purification steps, like liquid-liquid extraction, distillation and/or crystallization. We tried to evaluate if the required specifications may be reached by the implementation of preparative chromatography. For this chromatography was studied in details as unitary operation, in order to better understand separation mechanisms of studied compounds and implementation parameters. Two resin types were mainly used, a strong cationic one and a strong anionic one. Firstly, thermodynamic study of the adsorption of three organic acids in pure solution was performed. It revealed very different performances for both resins: adsorption on strong cationic resin is quite linear, whereas on strong anionic one adsorption is strongly nonlinear and fits with Langmuir model. Elution velocity influence on peak shape and so on dispersion was then studied. Column efficiency decreases linearly with elution velocity, accordingly to Van Deemter model. It was shown that the line slope was identical at lab scale and on a pilot ten times bigger. Then it may be used to predict column efficiency evolution during scale-up. Mixing solutions from synthetic or real origin were studied, to evaluate operational parameter influence on the separation, as load, feed concentration, pH… On the strong anionic resin, a first modeling was developed for experimental results. It highlighted that Langmuir type adsorption mechanism is not able to explain peak shape and position. We supposed that an ion exchange mechanism with the organic acid dissociated part may happen. This exchange may have a significant impact on peak shape and position, even if organic acids are mainly in molecular form, because of a low work pH. 4 Separations established at lab scale were validated at pilot scale in continuous chromatography ISMB. It was demonstrated that the anionic resin allows to reach a higher productivity than the cationic one, with a similar productivity. A complete purification process was tested with succinic acid, using bipolar electrodialysis acidification, reverse osmosis concentration, preparative chromatography separation with a strong anionic resin and nanofiltration discoloration. Product was then crystallized, to be compared to an industrial product. Our crystals were close to waited specifications and relatively better than the industrial ones. An additional ion exchange step could have allows to reach polymer grade. We show that chromatography is useful in an organic acid purification process, in order to reach a very high purity
Forrer, Nicola. "Antibody purification with ion-exchange chromatography." Zürich : ETH, 2008. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17784.
Full textFerreira, Filipe Miguel Garcia. "Antibodies purification using centrifugal partition chromatography." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/22486.
Full textA dificuldade em desenvolver antibióticos mais eficientes, numa altura em que a resistência microbiana tem vindo a aumentar, torna essencial o desenvolvimento de terapias alternativas, económicas e eficazes. Os anticorpos obtidos a partir da gema de ovo de galinha, imunoglobulina Y (IgY), têm-se destacado não só pela sua produção mais simples e em maior quantidade em relação aos anticorpos policlonais de mamífero, mas também devido às inúmeras vantagens em termos de aplicações. No entanto, atualmente não existe uma plataforma de purificação de IgY que seja económica, eficaz e passível de aplicação a nível industrial - uma lacuna que este trabalho se propõe a resolver. Assim, neste trabalho, estudou-se a possibilidade da utilização de sistemas aquosos bifásicos (SAB) compostos por PEG 1000 e tampão fosfato (K2HPO4/KH2PO4) ou K2HPO4, seguidos de um passo de ultrafiltração, ou acoplados à tecnologia de cromatografia de partição de força centrífuga (CPC), para a purificação de IgY. Foram avaliados os efeitos de pH (5,5; 6,0; 6,5; 7,5; e 8,0) e composição de PEG e sal na extração de IgY, bem como as condições utilizadas na CPC (fluxo da fase móvel, rotação e modo de operação). Foi estudada a estabilidade do anticorpo em soluções aquosas dos componentes utilizados nos SAB utilizando dicroísmo circular, assim como a atividade/estabilidade do anticorpo após o processo de purificação por ELISA, salientando assim o efeito do PEG 1000 na estrutura secundária e na atividade do IgY. O ATPS constituído por 18 % PEG 1000 + 13 % tampão fosfato a pH 6,0 conduz aos melhores resultados em termos de purificação, obtendo-se num único passo de extração uma pureza de IgY de 39 %. Após a aplicação de CPR, obteve-se IgY com um grau de pureza de 51 %, e com ultrafiltração, IgY com um grau de pureza de 47 %. Face aos resultados obtidos, destaca-se a CPR como a técnica mais adequada dado que permite obter IgY com um maior grau de pureza e ser passível de aplicação à escala industrial.
The difficulty in developing more effective antibiotics, at a time where the microorganism’s resistance to them has been increasing, turns essential the development of cheaper and effective alternative therapeutics. Antibodies obtained from the chicken’s egg yolk, immunoglobulin Y (IgY), have stood out because of their production simplicity and production in higher quantity when compared to mammal polyclonal antibodies, and also because of their advantages in terms of applicability. Nonetheless, there is still no low-cost, effective and scalable platform for the IgY purification - a vacuity that this work aims to solve. Therefore, in this work, the possibility of using aqueous biphasic systems (ABS) composed of PEG 1000 and phosphate buffer (K2HPO4/KH2PO4) or K2HPO4, followed by an ultrafiltration step, or coupled with centrifugal partition chromatography (CPC), was studied. The effect of pH (5.5; 6.0; 6.5; 7.5 and 8.0) and the PEG + phosphate buffer composition in the extraction of IgY was investigated, as well as the conditions to be used in CPC (mobile phase flow rate, rotation, and the operation mode). The stability of the antibody in aqueous solutions of the components used in the ABS formation was studied using circular dichroism, as well as the activity/stability of the antibody after the purification process, by ELISA, primarily outlining the effect of PEG 1000 in the secondary structure and activity of IgY. The ABS composed of 18 wt % PEG 1000 + 13 wt % phosphate buffer at pH 6.0 yielded the best results in terms of purification, achieving a 39 % IgY purity in a single extraction process. After the application of CPC, an IgY purity of 51 % was obtained, and with the ultrafiltration technique a purity of 47 % was obtained. According to these results, CPC appears as the most adequate purification technique due to the higher purity of IgY obtained and possibility of being applied at an industrial level.
Stevenson, Steven A. "Chromatography and purification of endohedral metallofullerenes." Diss., Virginia Tech, 1995. http://hdl.handle.net/10919/29176.
Full textPh. D.
Шебедя, Дмитро Сергійович. "Технологія виробництва субстанції моноклональних антитіл, специфічних до білку HBsAg вірусу гепатиту B. Дільниця культивування." Bachelor's thesis, КПІ ім. Ігоря Сікорського, 2020. https://ela.kpi.ua/handle/123456789/41083.
Full textDiploma work: 135 p., 26 figures, 12 tables, 13 formulas, 90 references. The work is devoted to the unification and improvement of the technology of mass production of monoclonal antibodies in the bioreactor, by immobilization of producer cells on the surface of semipermeable membranes and perfusion type of nutrient medium. Based on comparative characteristics, the main producer was selected by the most stable and highly immunogenic strain of hybridoma cells obtained by fusion of myeloma cells and splenocytes of mouse origin. Strain 95E1 is characterized by a titer of 1: 1000 in the culture fluid and an isotope of Ig G2α antibodies.Presented data describes the main stages of technology with biochemical characteristics and physicochemical parameters. From between of the designs of bioreactors used in the manufacturing technology, based on the index of a high yield of the target product, was chosen membrane bioreactors with hollow fiber cartridges type. In result of using the real model of the bioreactor as prototype, the technological, structural and hydraulic calculations were performed in order to modernize the technological parameters and production indicators.
Fargues, Claire. "Chromatographie des protéines appliquée à la purification de la pénicilline acylase : Modélisation de la colonne d'adsorption sur un gel d'hydroxyapatite." Vandoeuvre-les-Nancy, INPL, 1993. http://www.theses.fr/1993INPL014N.
Full textNourichafi, Nadia. "Purification des immunoglobulines plasmatiques humaines par chromatographie à partir de la fraction II+III de Cohn." Nancy 1, 1993. http://www.theses.fr/1993NAN19429.
Full textTHEOBALD, JEROME Weber Jean-Victor. "LES FULLERENES : PREPARATION ET PURIFICATION PAR CHROMATOGRAPHIE LIQUIDE PREPARATIVE /." [S.l.] : [s.n.], 1995. ftp://ftp.scd.univ-metz.fr/pub/Theses/1995/Theobald.Jerome.SMZ9544.pdf.
Full textThéobald, Jérôme. "Les fullerènes : préparation et purification par chromatographie liquide préparative." Metz, 1995. http://docnum.univ-lorraine.fr/public/UPV-M/Theses/1995/Theobald.Jerome.SMZ9544.pdf.
Full textOur purpose was the study of the production and the purification of fullerenes. After summarizing the bibliographic advances in the field of fullerenes production, synthesis mechanisms hypotheses and usual analysis methods, we focuse on the parameters which are expected to step in these molecules discovered recently : the simultaneous presence of aromatic molecules and warmth. In the second part of this thesis, we perform laser ablation experiments on various carbonaceous materials and show the link between the aromatic behavior of the target and the nature of fullerenes. We also propose original fullerenes sources, as polystyrene and toluene combustion or fullerenes recovery in carbonaceous wastes of some industrial processes. The production methods of fullerenes and particularly those presented in this work, lead to complex, mixtures of fullerenes and polycyclic aromatic compounds (PAC). We propose solutions for extraction, pre-purification for partial PAC elimination and separation of fullerenes. We develop a convenient method easy to be scaled-up. We present production forecasts and cost of fullerenes preparative scale separation
Emanuelsson, Ida, Anna-Karin Jansson, and Katarina Risö. "Characterising of chromatography gels for purification of erythropoietin." Thesis, Högskolan i Borås, Institutionen Ingenjörshögskolan, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-19097.
Full textUppsatsnivå: C
Amarouche, Nassima. "Développement de nouvelles méthodologies en Chromatographie de Partage Centrifuge (CPC) : Application à l’isolement et la purification des peptides pharmaceutiques." Thesis, Reims, 2013. http://www.theses.fr/2013REIMP205.
Full textThe work presented in this thesis deals with the development of new methodologies for the purification of pharmaceutical peptides by centrifugal partition chromatography (CPC) in order to introduce this technique as a tool for R & D but also in industrial production. The original character of this work relies on the introduction of new solvent systems and the development of new purification processes based on the co-current CPC mode. The different aspects of the process intensification and industrialization have also been studied.In the first part of the work, a study of some new aspects of the interest of the application of the stationary phase co-current mode in CPC is described. An original method for the purification of non-ionic tensioactive peptides in the co-current CPC mode was developed. This method has been successfully applied to the purification of a modified cyclosporine showing a therapeutic interest. This particular elution mode, taking advantage of the liquid nature of the stationary phase, appears to be an efficient solution to get round some hydrodynamic instabilities that sometimes appears during a purification intensification by CPC. A fundamental study of the effect of the peptide on the hydrodynamic behavior of the two phases in the separation and visualization of flow patterns within the CPC column allowed highlighting the role of the peptide in the disruption of phases composition of the chromatographic system. Other aspects of the interest of the co-current mode in CPC were investigated in this study, including the improvement of the efficiency and the resolution of the separation.The second part of the work focused on the development of new biphasic solvent systems particularly suitable for the purification of hydrophobic non-ionic peptides, including protected intermediates, which are very poorly soluble in the most common solvents used in chromatography. Two new scales of biphasic solvent systems showing a wide range of polarity and a ternary biphasic system were introduced to overcome solubility problems often encountered with synthetic hydrophobic protected peptides. The originality of these systems relies on the use of green solvents with high solvating character such as Methyl-THF and cyclopentyl methyl ether (CPME). The developed systems have been effectively used for the purification in CPC of a 39mer protected exenatide and and a 8mer protected peptide intermediate of bivalirudin synthesis
Seddas, Abdessamad. "Purification du mycoplasma-like organism (mlo) de la flavescence dorée de la vigne par immunoaffinité. Intégrité physique et biologique. Etude des principaux constituants." Dijon, 1994. http://www.theses.fr/1994DIJOS024.
Full textBrouchon, Julie. "Chromatographie cellulaire d'affinité : étude expérimentale des mécanismes de capture spécifique et implications pour un développement industriel." Thesis, Paris 6, 2015. http://www.theses.fr/2015PA066335/document.
Full textDevelopment of cell sorting system based on affinity chromatography for medical application is the goal of this thesis. We study mechanisms responsible for specific cell capture in chromatographic column. First an experimental study on system model emphasize the importance of the transport step, which allows the encounter between cells and surface of capture. Cell sedimentation in the main way of transport. That means flow velocity is a key parameter to optimize this transport step. Then the whole cell capture is studied : encounter and specific link formation. Depending on the flow velocity, there are two regimens. Until a velocity of 10-3 m.s-1, kinetics of capture is governed by encounter kinetics. For higher velocity, only fraction of encounters leads to cell capture. The capture kinetics depends not only on transport, but also on kinetics of formation of specific link. This experimental study allows us to design cell affinity chromatography depending on the need of each application. Especially, cell sorting at industrial scale is conceivable concerning the separation duration and column dimensions
Pezzini, Jérôme. "La chromatographie en mode mixte pour la purification de protéines recombinantes à visée santé : caractérisation des interactions impliquées dans les supports de chromatographie HyperCel®, modélisation et applications." Thesis, Bordeaux 2, 2011. http://www.theses.fr/2011BOR21885/document.
Full textMixed mode chromatography is the most innovative technique for bioseparation. Mixed mode resins, as the term suggest, involves multiples types of interaction at the same time. HyperCel mixed mode resins, HEA, PPA and MEP, involve aliphatic, aromatic or thiophilic groups as well as protonable amine located in the spacer arm or as a head group. Using classical chromatographic experiments, standards proteins and complex mixtures, we highlighted the two major types of interactions involved: hydrophobic and electrostatic interactions. We specifically influenced these interactions by modifying the environment in terms of ionic strength, pH, salt types, and other compounds. The combination of these interactions during every phase of a chromatographic process has been demonstrated. Mixed mode resins thus offer unique selectivity that can be controlled by the environment. This allowed us to develop several applications from antibodies fragments capture from insect cells, to the purification of MBP-tagged proteins, through monoclonal antibody capture from CHO cells. We thus enhanced mixed mode chromatography
Yu, Xiao-Jie. "Chromatographie liquide d'affinité de la thrombine sur résines de polystyrène modifié." Paris 13, 1987. http://www.theses.fr/1987PA132003.
Full textCastiblanco, Adriana P. "Expression and Purification of Engineered Calcium Binding Proteins." Digital Archive @ GSU, 2009. http://digitalarchive.gsu.edu/chemistry_theses/20.
Full textBoudesocque, Leslie. "Nouvelles méthodologies de purification de peptides par chromatographie de partage centrifuge : application à l'isolement et à la purification de peptides bio-actifs." Reims, 2010. http://theses.univ-reims.fr/sante/2010REIMP205.pdf.
Full text@This work presented in this manuscript deal with the valorization of alfalfa (Medicago sativa) peptide pool. In the first part of the work, the development of a new methodology for peptide purification using Centrifugal Partition Chromatography (CPC) is described. This process uses the ion exchange mode. A versatile methodology for peptide purification using a new mixed ion exchange mode was developed. A homogeneous or divided, with different exchanger activation rates, stationary phase can also be used. A fundamental study of the diffusion behavior of the exchanger in the solvent system, by NMR DOSY, confirmed the hypothesis of the ion pairs. The application of the mixed ion exchange process, whether for the capture of a bioactive peptide within a complex matrix, or for polishing a peptide of pharmaceutical interest, was successful. Fractionation of alfalfa's extracts generated fractions which were then biologically evaluated as an anti-ageing agent. The purification of an anti-HIV modified cyclosporine is also presented in this work. The second part of the work focused on alfalfa extracts screening as anti-ageing agent. Skin ageing is dependent on many molecular events initiated in part by free radicals, and amplified by activation of a particular class of enzymes: the Matrix Metalloproteases (MMPs). The potential antioxidant and inhibitory activity towards MMPs were then evaluated. A crude extract demonstrates significant antioxidant activity and inhibitory activity towards key enzymes of skin ageing: the MMP-1 and -3
Andersson, Mikael. "Characterisation of Chromatography Media Aimed for Purification of Biomolecules." Doctoral thesis, Uppsala universitet, Analytisk kemi, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-234743.
Full textHey, Carolyn McKenzie. "Antibody Purification from Tobacco by Protein A Affinity Chromatography." Thesis, Virginia Tech, 2010. http://hdl.handle.net/10919/42645.
Full textMaster of Science
Fernando, Samantha. "Monoclonal antibody (mAb) purification by counter current chromatography (CCC)." Thesis, Brunel University, 2011. http://bura.brunel.ac.uk/handle/2438/6522.
Full textHsu, Kuang-Hsin. "Scale-up of affinity chromatography for protein purifications." Ohio : Ohio University, 2000. http://www.ohiolink.edu/etd/view.cgi?ohiou1172002240.
Full textHidayah, Siti Nurul [Verfasser]. "Improvement of liquid chromatography for analysis and purification of proteoforms via rational protein purification parameter screening and sample displacement chromatography / Siti Nurul Hidayah." Hamburg : Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky, 2021. http://d-nb.info/1241743037/34.
Full textRing, Ludwig. "Purification of psychoactive biomolecules in plants using size exclusion chromatography." Thesis, Linköping University, Department of Physics, Chemistry and Biology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-18434.
Full textSize exclusion chromatography (SEC) was applied for purification of psychoactive biomolecules from plants. These molecules are in the same molecular weight range, but do not necessarily share other chemical properties, that makes the SEC technique efficient. By applying SEC as a first purification step much of the co-extractives from the plants can easily be removed. Large amounts of target substance can be obtained with little effort if the system is automated. Combining SEC with a second purification step, consisting of normal phase chromatography, provides high purity of the target substance.
Both known and unknown psychoactive biomolecules can easily be purified using the purification method developed in this Master's Thesis. Purifications that previously required long time and much "hands-on" can be completed much faster and with less manual work.
The method developed was tested on cannabis, coffee and 'Spice' with good results.
McElhiney, Jacqueline. "Purification, detection and biological effects of cyanobacterial toxins." Thesis, Robert Gordon University, 1999. http://hdl.handle.net/10059/528.
Full textKarnchanasri, Kritsadanchalee. "Bi-layered chromatography matrices for the purification of biological nanoplexes." Thesis, University of Birmingham, 2013. http://etheses.bham.ac.uk//id/eprint/4149/.
Full text