Journal articles on the topic 'Chlorella pyrenoidosa Growth'

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1

Sun, Jia, Guo Ping Yu, and Yue Li. "The Uranium Biosorption by Chlorella Pyrenoidosa and its Physiological Responses to Uranium." Advanced Materials Research 610-613 (December 2012): 40–43. http://dx.doi.org/10.4028/www.scientific.net/amr.610-613.40.

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The effect of uranium at various concentration on the growth of Chlorella pyrenoidosa and the resistance of Chlorella pyrenoidosa to uranium were investigated in this paper. The results showed that the content of O2- and the activity of CAT arose when the concentration of uranium varied at the range of 0.1 to 1.0 mg/L. Uranium had no negative effect on the growth of Chlorella pyrenoidosa at the concentration of 0.1 to 0.5 mg/L, however, had positive effect to some extent. And the rise of the content of O2- and the activity of CAT was followed by rapidly returning to the normal level. The uranium at the concentration of 1.0 mg/L prolonged the lag period of the growth of Chlorella pyrenoidosa. The growth of Chlorella pyrenoidosa arose negatively, the content of O2- also arose to an unreturnable level and the activity of CAT was lower than CK when the concentration of uraniumm varied at the range of 5.0 to 10.0 mg/L. The uranium biosorption rate by Chlorella pyrenoidosa reached to the highest value of 70.43%when concentration of uraniumm was 0.5 mg/L. Chlorella pyrenoidosa is resistant to uranium of low concentration(≤1.0mg/L)and its biosorption rate is rather high, which indicated good prospect in its future application.
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2

Guo, Hancheng, and Zhiguo Fang. "Effect of light quality on the cultivation of chlorella pyrenoidosa." E3S Web of Conferences 143 (2020): 02033. http://dx.doi.org/10.1051/e3sconf/202014302033.

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Effect of light quality, including red light, blue light, white light, red and blue mixing light with 8:1, 8:2 and 8:3, on the growth characteristics and metabolite accumulation of chlorella pyrenoidosa was conducted based on light emitting diode (LED). Results showed that chlorella pyrenoidosa grew best under blue light, and the optical density, specific growth rate and biomass of chlorella pyrenoidosa was about 2.4, 0.10 d-1 and 6.4 g·L-1, respectively, while the optical density of chlorella pyrenoidosa was between 1.0 and 1.7, specific growth rate was between 0.06-0.10 d-1 and biomass was between 2.7 and 3.8 g·L-1 under other light quality after 30 days of cultivation. The optical density, specific growth rate and biomass of chlorella pyrenoidosa was approximately 2.05 times, 1.33 times and 2.06 times under blue light than red light, respectively. Moreover, Red and blue mixing light was conducive to the synthesis of chlorophyll a and carotenoids of chlorella pyrenoidosa, and blue light could promote the synthesis of chlorophyll b. Chlorophyll a and carotenoids content of chlorella pyrenoidosa was 13.5 mg·g-1and 5.8 mg·g-1 respectively under red and blue mixing light with 8:1, while it was 8.4 mg·g-1 and 3.6 mg·g-1 respectively under blue light. Red and blue mixing light was more conducive to protein and total lipid content per dry cell of chlorella pyrenoidosa. Protein and total lipid content was 489.3 mg·g-1 and 311.2 mg·g-1 under red and blue mixing light with 8:3, while it was 400.9 mg·g-1 and 231.9 mg·g-1 respectively under blue light.
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3

Zhong, Yun Na, and Ya Lei Zhang. "Influence of Contaminative Bacteria during Cultivation of Chlorella pyrenoidosa in Soybean Processing Wastewater." Advanced Materials Research 666 (February 2013): 27–31. http://dx.doi.org/10.4028/www.scientific.net/amr.666.27.

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Bacteria pollution is easy to happen during the treatment of wastewater by Chlorella pyrenoidosa (C. pyrenoidosa). And it is the key technical problem in the engineering applications. It is necessary to find out the influence of bacteria during the process, and then formulate the control project of bacteria pollution. This paper mainly studied on the relationship between C. pyrenoidosa and bacteria, during the treatment of bean products wastewater. Comparing those relationship between three stains of bacteria and C. pyrenoidosa, we found that three stains of bacterial all have adverse impact on the growth of chlorella and adipose accumulate. And those three stains of bacterial have no definite influence on C. pyrenoidosa. According to the above results, the control of bacteria during the wastewater-treatment by C. pyrenoidosa should be based on the type of wastewater and the follow-up using of chlorella.
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4

Zhang, Zhan Ning, and Jian Sheng Cui. "The Effects of Pb2+ Stress on the Growth and Physiological Characteristics of Chlorella pyrenoidosa." Advanced Materials Research 1073-1076 (December 2014): 147–53. http://dx.doi.org/10.4028/www.scientific.net/amr.1073-1076.147.

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In the Pb2+ stress experiments, the concentrations of Pb2+ were 1, 5, 10, 50, and 80 mg/L respectively, BG11 culture medium was served as the control. The results showed that Pb2+ affected markedly on the growth and physiological characteristics of chlorella pyrenoidosa under certain concentration. As the exposure time, chlorella pyrenoidosa biomass is restrained gradually. Pb2+ also have significant inhibition to effect chlorophyll content and have an effect relationship with the malondialdehyde content of chlorella pyrenoidosa . Lead concentrations and the soluble protein content are time effect relationship . And the longer the inhibitory effect becomes more obvious. Superoxide dismutase (sod) activity increased first then decreased with the increase of the concentration of Pb2+.
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5

Fu, Hai Yan, Ming Hou, Tian Chai, Guo He Huang, Peng Cheng Xu, and Ya Li Guo. "Allelopathy Effects of Various Higher Landscape Plants on Chlorella pyrenoidosa." Advanced Materials Research 518-523 (May 2012): 23–28. http://dx.doi.org/10.4028/www.scientific.net/amr.518-523.23.

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In this paper, the effects of the culture water of three types of higher landscape plants Typha latifolia L, Canna indica L and Iris tectorum Maxim on the growth of Chlorella pyrenoidosa have been investigated. The results show that Iris culture water promoted the growth of Chlorella pyrenoidosa, which was facilitated at low contentions and inhibited at high concentrations by the culture water of Canna indica L. Moreover, inhibitory effect of the culture water of Typha latifolia L was the strongest with the rate of 50.7%. Differences of the effects of the three plants on the chlorophyll a contents of Chlorella pyrenoidosa were significant (P Iris tectorum Maxim was utilized, whereas the content was lowest at merely 41% of that of the control group when treated with 80% of the culture water of Typha latifolia L.
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6

Rismiarti, Asih, Hermin Pancasakti Kusumaningrum, and Muhammad Zainuri. "Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan Chlorella vulgaris Menggunakan 18SrDNA." Bioma : Berkala Ilmiah Biologi 18, no. 2 (August 8, 2016): 30. http://dx.doi.org/10.14710/bioma.18.2.30-40.

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Chlorella pyrenoidosa is a unicellular green algae that grows in fresh water with carotenoids consisting of β-carotene, α-carotene, anthaxanthin, neoxanthin, zeaxanthin and lutein. C. vulgaris usually it lives in sea water with carotenoids, chlorophyll, tocopherol, ubiquinone and proteins. The quality of them is improved by protoplast fusion and identified further using moleculer analysis. This study aims to find out the characterization and identification of molecular fusan that is obtained from interspecies C. pyrenoidosa and C. vulgaris protoplast fusion process using 18SrDNA. Both C. pyrenoidosa and C. vulgaris are combined by protoplast fusion and then they were performed the isolation of DNA with CTAB modification method, followed by PCR gradient using primers 18S Chlorella and performed DNA sequencing. The result show that there are different characters between masterplan and fusan based on growth of fresh water and sea water medium. The success frequency of fusan as a result from protoplast fusion in the fresh water media is 21% and 6% for sea water medium. The results of the alignment between fresh water fusan and C. vulgaris masterplan from GeneBank shows that the base sequence homology is 93% C. pyrenoidosa masterplan from GeneBank is 90%. The result of molecular identification towards the sequence of fresh water fusan bases shows that there is a kinship relationship with the masterplan of C. pyrenoidosa 18S Chlorella and Chlorosphaera klebsii microalgae compared with some other species from Chlorophyta group with similarity value as many as 91%. It shows that the high variety genetic is based on variations of the base sequence and has a kinship with other species in the Chlorophyta group. Keywords : Chlorella pyrenoidosa, Chlorella vulgaris, Protoplast Fusion, DNA Sequensing, 18SrDNA.
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7

Megawati, Megawati, Astrilia Damayanti, Zuhriyan Ash Shiddieqy Bahlawan, Erma Nurunia, Fatkhulil Jannah Eva Agustina, Fidyawati Fidyawati, and Hanifah Hanifah. "GROWTH RATE AND BIOCHEMICAL CHARACTERIZATION OF CHLORELLA PYRENOIDOSA CULTIVATED IN SUGARCANE VINASSE MEDIUM." ASEAN Engineering Journal 12, no. 2 (June 1, 2022): 127–34. http://dx.doi.org/10.11113/aej.v12.17156.

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Chlorella pyrenoidosa is a microalgae species that contains proteins, carbohydrates, amino acids, carotenoids, vitamins, and minerals. Due to its compounds, the researchers have attempted to make bioethanol using C. pyrenoidosa through a biorefinery approach. However, the ratio of bioethanol production towards the raw material needs of C. pyrenoidosa is still small because of its low carbohydrate content. Thus, in this research, vinasse is used as its growth medium to increase the carbohydrate content. The research objective is to study the effect of vinasse volume ratio and nutrient addition towards the size, optical density, carbohydrate composition, growth rate of the C. pyrenoidosa, and its evaluation as a biorefinery raw material. C. pyrenoidosa was cultivated in freshwater and vinasse (20 and 30% v/v) in mini ponds, equipped with lighting using 3280 lumens lamp, aeration with air, and Guillard as nutrient. In vinasse, the cultivation was done with and without periodic nutrient additions. The microalgae cell size was increased if cultivated in vinasse and given Guillard addition, which is 3.0-3.6 mm (in freshwater), 4.1-8.6 mm (in vinasse with nutrient every 2 days), 4.8-6.3 mm (in vinasse without nutrient every 2 days). The microalgae carbohydrate composition cultivated in vinasse was sharply increased compared to in freshwater. Thus, C. pyrenoidosa cultivated in vinasse is very potential for bioethanol production. Specific growth of C. pyrenoidosa in vinasse with nutrient is faster (0.087 day-1) than without nutrient (0.023 day-1) and in freshwater (0.062 day-1). Cultivated C. pyrenoidosa contains proteins, lipids, and carbohydrates, so it has the potential of becoming a biorefinery raw material.
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8

Kusumaningrum, Hermin Pancasakti, Muhammad Zainuri, Indras Marhaendrajaya, Agus Subagio, Widianingsih Widianingsih, Hadi Endrawati, Annisa Fadillah, Muhammad Iskandar Zulkarnain, Yuvita Muliastuti, and Imam Misbach. "Effect of Silver Nano Particle Microalgae Chlorella pyrenoidosa and Dunaliella salina on Growth and Survival of Penaeus monodon Larvae." Buletin Oseanografi Marina 10, no. 2 (April 29, 2021): 105–11. http://dx.doi.org/10.14710/buloma.v10i2.35483.

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Penaeus monodon is one of the most important farmed crustaceans. Its also known as Asia Tiger Shrimp because its carapace and abdomen are transversely banded with red and white. The use of synthetic antibiotic in aquaculture had caused problems related to health and environmental safety. Chlorella pyrenoidosa and Dunaliella salina are photosynthetic microalgae. Silver nano particle in microalgae C. pyrenoidosa and D. salina had synthesized and showed their growth stability. They offer a potency to be exploited to supported growth and survival of shrimp larvae. The objective of the study was the application of silver nano particle in microalgae C. pyrenoidosa and D. salina on P. monodon larvae. The research methodology was carried out by making microalgae C. pyrenoidosa and D. salina containing silver nano particle and used as feed of shrimp larvae. Observations were made on the growth and survival of shrimp larvae compared to both microalgae and common feed. The results showed that the P. monodon larvae have the higher growth and survival rate with microalgae C. pyrenoidosa at the beginning of their growth compared to D. salina. However, microalgae without nanosilver and common feed showed a better result for growth and activity of shrimp larvae.
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9

Tian, Jian Ru, Guo Li Chen, Guang Feng Kang, and Xiu Ping Zheng. "Bioavailability of Phosphorus Fractions for Incepting of Chlorella pyrenoidosa in the Lower Reaches of the Hanjiang River in China." Advanced Materials Research 183-185 (January 2011): 3–7. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.3.

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Using total phosphorus (TP) concentrations to estimate eutrophication risk is problematic for management purposes, as only some forms of P are biologically available for phytoplankton growth. This study estimated the bioavailability of P fractions of water and the floodplain sediment in the lower reaches of the Hanjiang River in China using multiple regressions. And the effects of concentrations of P fractions on algal growth were also studied. The results showed that the total soluble P (TSP) and the particulate P (PP) can well predict the growth of Chlorella pyrenoidosa in water samples. And the exchangeable P (exch-P) and the P bound to Al, Fe, and Mn oxides and hydroxides (Fe/Al–P) were adapted to predicting the growth of Chlorella pyrenoidosa in the floodplain sediment samples.
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10

Li, Q., Y. Y. Wu, and Y. D. Wu. "Effects of fluoride and chloride on the growth of Chlorella pyrenoidosa." Water Science and Technology 68, no. 3 (August 1, 2013): 722–27. http://dx.doi.org/10.2166/wst.2013.279.

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To compare the toxic action of fluoride (F−) and chloride (Cl−) on freshwater algae, effects of F− and Cl− on Chlorella pyrenoidosa growth were investigated by determination of the algal biomass and model analysis. Results showed that the exponential growth equation (D = D0 + aebT) may be used to fit the relationship between cell density (D) of C. pyrenoidosa and exposure time (T), but F− and Cl− have significantly different effects on parameters a (initial quantity of algae at the exponential growth stage) and b (growth constant). In the range of experimental concentrations, F− inhibited C. pyrenoidosa growth, and percent inhibition increased with increasing exposure time and F− concentration. By contrast, Cl− either inhibited or enhanced C. pyrenoidosa growth depending on Cl− concentration and exposure time, and percent inhibition increased with increasing Cl− concentration but decreased with increasing exposure time. The dose–response equation: I = min + (max − min)/(1 + 10lgEC50−lgC) may be used to fit the relationship between percent inhibition (I) and F− or Cl− concentration (C); however, F− was distinct from Cl− in terms of effects on parameter ‘min’. The EC50 values of F− and Cl− ranged from 118 to 170 mg/L and 19.73 to 36.33 g/L, respectively; the latter is much higher than the former. Thus F− has higher toxicity than Cl−.
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11

Li, Feng-Min, and Hong-Ying Hu. "Isolation and Characterization of a Novel Antialgal Allelochemical from Phragmites communis." Applied and Environmental Microbiology 71, no. 11 (November 2005): 6545–53. http://dx.doi.org/10.1128/aem.71.11.6545-6553.2005.

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ABSTRACT Antialgal allelochemicals were isolated from Phragmites communis Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of Chlorella pyrenoidosa and Microcystis aeruginosa but had no inhibition on Chlorella vulgaris. The 50% effective concentrations (EC50) of the allelopathic fractions on C. pyrenoidosa and M. aeruginosa were 0.49 and 0.79 mg/liter, respectively. The allelopathic activity of the fraction was species-specific. The isolated allelopathic fraction caused metal ion leakage from algal cells. The fraction decreased the activities of antioxidant enzymes, such as superoxide dismutase and peroxidase. The addition of the isolated fraction increased the concentration of unsaturated lipid fatty acids in cell membrane of C. pyrenoidosa and M. aeruginosa. This caused a change in plasma membrane integrity and the leakage of ions in the protoplast. The allelopathic compound was identified by nuclear magnetic resonance and gas chromatography-mass spectrometry as ethyl 2-methylacetoacetate. Synthesized ethyl 2-methylacetoacetate also showed allelopathic activity on C. pyrenoidosa and M. aeruginosa. The EC50 of synthesized ethyl 2-methylacetoacetate on C. pyrenoidosa and M. aeruginosa were 0.49 and 0.65 mg/liter, respectively.
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12

Lv, Kunling, Qingxia Yuan, Hong Li, Tingting Li, Haiqiong Ma, Chenghai Gao, Siyuan Zhang, Yonghong Liu, and Longyan Zhao. "Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro." Foods 11, no. 5 (March 1, 2022): 725. http://dx.doi.org/10.3390/foods11050725.

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This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food. C. pyrenoidosa polysaccharides were prepared and their physicochemical characteristics were determined. The digestibility and fermentation characteristics of C. pyrenoidosa polysaccharides were evaluated using in vitro models. The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara. C. pyrenoidosa polysaccharides could not be degraded under saliva and the gastrointestinal conditions. However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed. Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes. Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases. Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
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13

Yang, Qi Peng, Xiu Lin Wang, Xiao Yong Shi, Ke Qiang Li, and Li Hong Yue. "Study on Biological Fixation of High-Concentration CO2 Using Chlorella Pyrenoidosa." Advanced Materials Research 343-344 (September 2011): 361–67. http://dx.doi.org/10.4028/www.scientific.net/amr.343-344.361.

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CO2 emissions have serious impact on the world economy, especially at high-concentration. Green algae are known to have a tolerance to high CO2 level. In this paper, the rate of CO2 fixation using Chlorella Pyrenoidosa was analyzed in different environments. 1) Chlorella Pyrenoidosa fixed CO2 slowly in air, while this alga could rapidly grow in high-concentration CO2 until CO2 level was beyond 10%. At 25% CO2, the rate of CO2 fixation was lower than that at 10% CO2, but still 1.18 times as higher as in air. 2) At lower initial inoculation density of Chlorella Pyrenoidosa, its growth rate was relatively high but the rate of CO2 fixation was low. When initial inoculation density beyond 0.187×108cells/ml, the average rate of CO2 fixation was ranged from 2.786 gCO2/L·d to 2.847 gCO2/L·d. 3) During the five days, the average rate of CO2 fixation was 3.044 gCO2/L·d in NaNO3 resource. When NH4Cl or NaNO2 is regarded as N resource, the rate of CO2 fixation was almost neglectable.
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14

YUE, Xia-li, Xiao-qing YU, Yong-hong LIU, and Yuan-yan DONG. "Effect of Bensulfuron-Methyl on Growth of Chlorella pyrenoidosa." Agricultural Sciences in China 6, no. 3 (March 2007): 316–21. http://dx.doi.org/10.1016/s1671-2927(07)60051-0.

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15

Larson, Loren J. "Effect of phenolic acids on growth of Chlorella pyrenoidosa." Hydrobiologia 183, no. 3 (October 1989): 217–22. http://dx.doi.org/10.1007/bf00007242.

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16

Li, Junrong, Yingjun Wang, Na Li, Yan He, Hong Xiao, Dexin Fang, and Chao Chen. "Toxic Effects of Bisphenol A and Bisphenol S on Chlorella Pyrenoidosa under Single and Combined Action." International Journal of Environmental Research and Public Health 19, no. 7 (April 2, 2022): 4245. http://dx.doi.org/10.3390/ijerph19074245.

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Bisphenol A (BPA) is an important industrial chemical; bisphenol S (BPS) is a substitute for BPA. Both are frequently detected in rivers, sewage, and surface water, and have a great impact on the water environment. The effects of BPA and BPS on cell growth, chlorophyll a content, and oxidative stress of Chlorella pyrenoidosa (C. pyrenoidosa) were studied. When BPA and BPS acted alone or in combination, compared with the blank control group, the growth of C. pyrenoidosa in the experimental group showed a pattern of “low promotion and high inhibition”, and the inhibition rate reached the maximum on the 6th day. Under the combined action, the reactive oxygen species (ROS) level of C. pyrenoidosa first increased, and then decreased. In addition, the activity of superoxide dismutase (SOD) and peroxidase (POD) increased with the increase in combined concentration. In the 0.5 P treatment group, SOD and POD activity reached peak values of 29.59 U/mg∙prot and 1.35 U/mg∙prot, respectively. The combined toxicity of BPA and BPS to C. pyrenoidosa was evaluated as a synergistic effect by using toxicity unit and additive index methods. This study evaluated the effects of BPA and BPS on algae in the aquatic environment, providing some data support for their potential ecological risks.
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17

Liu, Lu, Shulin Zhang, Wei Dai, Xiangdong Bi, and Dajuan Zhang. "Comparing effects of berberine on the growth and photosynthetic activities of Microcystis aeruginosa and Chlorella pyrenoidosa." Water Science and Technology 80, no. 6 (September 15, 2019): 1155–62. http://dx.doi.org/10.2166/wst.2019.357.

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Abstract Berberine is a potent algicidal allelochemical of Microcystis aeruginosa. To optimize its application in the control of Microcystis blooms, the effects of berberine on the growth and photosynthetic activities of M. aeruginosa and a non-target green alga, Chlorella pyrenoidosa, were compared. The results showed that the algicidal activity of berberine on M. aeruginosa was light dependent. Berberine had no algicidal effects on C. pyrenoidosa with or without light exposure. Under light-dark conditions, berberine significantly decreased the chlorophyll fluorescence parameters in M. aeruginosa while no significant berberine-induced changes were observed under constant darkness. Significant reductions of photosystem II (PSII) and whole chain electron transport activities in M. aeruginosa exposed to berberine suggested that PSII was the important target site attacked by berberine. Contrary to M. aeruginosa, no berberine-induced inhibition in photosynthesis activities were observed in C. pyrenoidosa. The differences in photosynthetic apparatuses of these two algae might be responsible for their different sensitivities to berberine.
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18

Yang, Cong-fa, Zhong-yang Ding, and Ke-chang Zhang. "Growth of Chlorella pyrenoidosa in wastewater from cassava ethanol fermentation." World Journal of Microbiology and Biotechnology 24, no. 12 (August 25, 2008): 2919–25. http://dx.doi.org/10.1007/s11274-008-9833-0.

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19

Wan, Liang, Yixiao Wu, Xuemei Zhang, and Weihao Zhang. "Nutrient removal from pickle industry wastewater by cultivation of Chlorella pyrenoidosa for lipid production." Water Science and Technology 79, no. 11 (June 1, 2019): 2166–74. http://dx.doi.org/10.2166/wst.2019.217.

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AbstractThe present research examined the feasibility of cultivating Chlorella pyrenoidosa in pickle industry wastewater for simultaneous nutrient removal and lipid production. The characteristics of microalgae growth, nutrient removal, lipid accumulation and composition of C. pyrenoidosa cultivated in pickle wastewater with different dilution ratios were investigated. The results showed the maximum algae biomass concentration of 1.57 ± 0.12 g L−1 was achieved in non-diluted pickle wastewater with the highest biomass productivity of 170.65 mg L−1 day−1. Maximum nutrient removal efficiency was observed in 20.0% pickle wastewater with removal rates of chemical oxygen demand (COD), total phosphorus (TP), total nitrogen (TN) and NH4-N at 84.67%, 92.46%, 85.82% and 93.42%, respectively. The lipid content of C. pyrenoidosa growing in pickle wastewater ranged from 29.73% to 31.78%, with a highest lipid productivity of 57.23 mg L−1 day−1. The relative content of triolefinic acids (C16:3 and C18:3) decreased while the monoenoic acids (C16:1 and C18:1) increased synchronously with the pickle wastewater concentration. Unsaturated fatty acid methyl esters were the main components, ranging from 73.04% to 77.6%. The biodiesel properties satisfied the major specifications in US and European biodiesel standards. The results indicated that C. pyrenoidosa is a promising species for nutrient removal together with lipid production in pickle industry wastewater.
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20

Czerpak, Romuald, Andrzej Bajguz, Bożena Białecka, Lidia E. Wierzchołowska, and Małgorzata M. Wolańska. "Effect of auxin precursors and chemical analogues on the growth and chemical composition in Chlorella pyrenoidosa Chick." Acta Societatis Botanicorum Poloniae 63, no. 3-4 (2014): 279–86. http://dx.doi.org/10.5586/asbp.1994.038.

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In this paper the authors present studies on the effect of auxin precursors and chemical analogues on the growth and biochemical composition in <i>Chlorella pyrenoidosa</i> (<i>Chlorophyceae</i>). Among auxin precursors tryptamine exhibited slightly higher stimulative activity in regard to fresh and dry weight, mineral substances, chlorophylls, carotenoids, monosaccharides (aldohexoses) and water-soluble proteins content in <i>Ch. pyrenoidosa</i> cells as compared to anthranilic acid. Among auxin analogues used phenoxyacetic acid and naphthyl-3-acetic acid had the strongest stimulative effect of the above-mentioned parameters. Their activity was significantly higher than that of auxin precursors. The activity of naphthyl-3-sulphonic acid was slightly lower than that of tryptamine, whereas the stimulation by 2,4-dichlorophenoxyacetic acid was similar to that of anthranilic acid. In <i>Ch. pyrenoidosa</i> cells 2,4-dichlorophenoxyacetic acid and naphthyl-3-sulphonic reached their maximum activity at the latest (between the 15th or 16th day) of the culturing, whereas tryptamine, phenylacetic acid, naphthyl-3-acetic acid and indolyl-3-acetic acid - at the earliest (between the 8th or 12th) day.
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21

Elystia, Shinta, Sri Rezeki Muria, and Herta Furaida Erlangga. "Cultivation of Chlorella pyrenoidosa as a raw material for the production of biofuels in palm oil mill effluent medium with the addition of urea and triple super phosphate." Environmental Health Engineering and Management 7, no. 1 (January 13, 2020): 1–6. http://dx.doi.org/10.34172/ehem.2020.01.

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Background: The utilization of microalgae as a renewable energy is an important aspect in solving shortage of future oil reserve in 15 years. One of the renewable energy sources is microalgae biodiesel. Palm oil mill effluent (POME) is a wastewater that has a high content of organic materials. These organic materials can be used as growth nutrients for microalgae. Chlorella pyrenoidosa is one of the most potential microalgae used as a raw material for the production of biodiesel since it contains lipids (8%-35%). Methods: Chlorella pyrenoidosa was cultured on the POME medium with concentrations of 0%, 25%, 50%, 75%, and 100%v with addition of synthetic nutrients (urea: TSP) at the ratios of 2:1 ; 1:2 ; 0.5:1 in a 500 mL Erlenmeyer flask, at pH 6-8, aeration using aquarium pumps, and using LED lights (3000 lux). Results: It was revealed that at POME concentration of 25% and with addition of urea: TSP at a ratio of 2:1, the optimum specific growth rate (0.306/day) with the highest number of cells was 3.530 × 107 cells/mL and the highest lipid content was 36% of its dry weight. The removal efficiency of POME could be obtained from the removal efficiency of chemical oxygen demand (COD), total nitrogen, and orthophosphate (P-PO4 ), which was 70, 90.42, and 81.12%, respectively. Conclusion: According to the results, under appropriate culture conditions, C. pyrenoidosa can produce lipids with good use of nutrients contained in the POME medium.
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Duan, Chen, and Duan. "Transcriptional Analysis of Chlorella Pyrenoidosa Exposed to Bisphenol A." International Journal of Environmental Research and Public Health 16, no. 8 (April 16, 2019): 1374. http://dx.doi.org/10.3390/ijerph16081374.

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Bisphenol A (BPA) is the raw material of 71% of polycarbonate-based resins and 27% of epoxy-based resins which are used for coating metal-based food and beverage cans. Meanwhile, it is taken into account as a typical environmental pollutant. Hormesis may occur in algae exposed to BPA. In this study, the effects of BPA on Chlorella pyrenoidosa were assessed based on growth inhibition and transcriptome analysis. We have focused on two exposure scenarios as follows: (1) exposure to a low stimulation concentration (0.1 mg.L−1, 19.35% promotion in cell density on the 3rd day); (2) exposure to a high inhibition concentration (10 mg.L−1, 64.71% inhibition in cell density on the 3rd day). Transcriptome analysis showed enrichment in nucleotide transport, single-organism transport, cellular respiration. Among them, adenosine triphosphate (ATP) synthase and Nicotinamide adenine dinucleotide (NADH) dehydrogenase were upregulated under 0.1 mg.L−1 BPA treatment. These changes enhanced the physiological and energy metabolic pathways of C. pyrenoidosa, thereby stimulating cell proliferation. At exposure to the high BPA, severe inhibited changes in the expression levels of several pathways were observed, which were related to tricarboxylic acid (TCA) cycle, glycolysis, fatty acid metabolism, oxidative phosphorylation, and photosynthesis. Therefore, BPA could negatively affect growth inhibition through the multiple energy metabolism processes. These results may result in a deeper insight into BPA-induced biphasic responses in algae, and provide vital information to assess the potential ecological risks of exposure to BPA in an aquatic ecosystem.
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Azhar, Afriyanti, Abdi Dharma, Armaini Armaini, Zulkarnain Chaidir, Syafriza Yanti, and Nasril Nasir. "Integrasi Bioremediasi Limbah Peternakan Sapi dan Kultivasi Mikroalga Chlorella vulgaris dan Chlorella pyrenoidosae." Jurnal Katalisator 2, no. 2 (October 6, 2017): 67. http://dx.doi.org/10.22216/jk.v2i2.2127.

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<p>Dairy manure that functioned as organic fertilizer has potential impact as environmental destructive waste because the absorption of inorganic nutrients in the fertilizer. On the other hand, microalgae growth is hindered by the high cost of culture, whereas organic and inorganic nutrient requirements. Dairy manure have the potential for microalgae nutrients, but are hindered by turbidity levels. Its has been tested for dilution dairy manure and treatments on fluorescence and sunlight sources and the addition of urea commercial fertilizer to absorbs organic and inorganic nutrients. Its prove the integration of bioremediation of dairy manure and microalgae cultivation Chlorella pyrenoidosa and Chlorella vulgaris can be performed and also serves as a source of caratenoid (chlorophyll)</p>
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Moreno Osorio, Jairo Hernan, Angelo Del Mondo, Gabriele Pinto, Antonino Pollio, Luigi Frunzo, Piet Nicolaas Luc Lens, and Giovanni Esposito. "Nutrient removal efficiency of green algal strains at high phosphate concentrations." Water Science and Technology 80, no. 10 (November 15, 2019): 1832–43. http://dx.doi.org/10.2166/wst.2019.431.

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Abstract The effects of autotrophic and mixotrophic conditions on microalgae growth and nutrient removal efficiency from synthetic wastewater by different microalgae were investigated. Although several studies have demonstrated the suitability of microalgae technologies for ammonia-rich wastewater treatment, only a few have been used for treatment of phosphate-rich industrial wastewaters. In this work, six microalgae were cultivated in batch mode in a growth medium with a high phosphate concentration (0.74 Mm PO43−-P) and different carbon sources (ammonium acetate and sodium bicarbonate) without CO2 supplementation or pH adjustment. Their potential for nutrient removal and biomass generation was estimated. The biomass growth in the reactors was modeled and the data aligned to the Verhulst model with R2 &gt; 0.93 in all cases. Chlorella pyrenoidosa ACUF_808 showed the highest final biomass productivity of 106.21 and 75.71 mg·L−1·d−1 in media with inorganic and organic carbon sources, respectively. The highest phosphorus removal efficiency was 32% with Chlorella vulgaris ACUF_809, while the nitrate removal efficiency in all reactors exceeded 93%. The coupled cultivation of the novel isolated strains of C. pyrenoidosa and C. vulgaris under mixotrophic conditions supplemented with ammonium acetate might be a promising solution for simultaneous nitrate and phosphate removal from phosphorus-rich wastewaters.
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Tang, Jun, Jun Yang, Sirui Zhao, Xiaoyu Wang, and Zhengxin Xie. "Toxic Effects of Tetracycline and Its Removal by the Freshwater Microalga Chlorella pyrenoidosa." Agronomy 12, no. 10 (October 13, 2022): 2497. http://dx.doi.org/10.3390/agronomy12102497.

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The prevalence of tetracycline (TC) in aquatic environments has raised increasing concern due to its high ecotoxicology risk. The application of microalgae in the removal of antibiotics is a competitive alternative technology. However, the removal mechanism of TC by microalgae and its correlation with the ecotoxic response of microalgae are still not clear. In this study, the ecotoxicity of TC (0.5–10 mg L−1) and its removal by the freshwater alga Chlorella pyrenoidosa were investigated. The results show that TC has significant inhibitory effects on microalgal growth, photosynthetic pigment, and photosynthetic efficiency, with maximum inhibition rates of 49.68%, 62.54%, and 48.08%, respectively. However, the growth inhibition and photosynthesis impairment caused by TC seems to be reversible, as reflected by the decreased inhibition rates with prolonged exposure time. The simultaneous increases in the activities of superoxide dismutase (9.69–23.53%) and peroxidase (15.15–110.92%) and the contents of glutathione (17.62–64.17%) and malondialdehyde (25.16–35.25%) suggest that TC causes moderate oxidative stress. C. pyrenoidosa exhibits high removal efficiency (91.44–95.14%) for TC after 48 h of exposure with short half-lives of 11.31–13.48 h. Biodegradation (56.86–64.62%) is the primary removal mechanism of TC, accompanied by the abiotic process (24.68–40.97%), bioaccumulation (1.95–10.97%), and bioadsorption (0.09–0.38%). These findings demonstrate the toxicity resistance and high removal capacity of C. pyrenoidosa to TC, highlighting its potential application in the remediation of TC-contaminated water.
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Silaen, Srinatalia, and Welmar Olfan Basten Barat. "The Effect of Water Quality on Growth of Microalgaes Chlorella pyrenoidosa." Journal of Applied Geospatial Information 6, no. 2 (August 22, 2022): 638–40. http://dx.doi.org/10.30871/jagi.v6i2.4396.

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The diversity of microalgae on earth is very high, the high diversity of microalgae allows us to obtain microalgae that have the potential to produce large amounts of oil. In addition, the use of microalgae as a source of biodiesel does not interfere with the food supply. This is because microalgae do not compete with food. The results of the analysis showed that the parameters that passed the water quality standard were BOD in the range of 0.990-90.76 mg/l, COD in the range of 3.093 -283 mg/l, and phosphate in the range of 0.01-0.21 mg/l. Judging from the color and smell, the waters that are closer to the cages will be greener in color accompanied by the smell of fish mixed with fishy feed and feel thick. COD content ranged from 3.093 mg/l-269.5 mg/l. This is caused by the high concentration of organic matter in the waters. The quality of the waters of Lake Toba which is classified as not good is the measurement of color, smell, taste, BOD, COD, and Phosphate in the waters. Which are classified as good temperature, turbidity, pH, Ammonia, and Nitrate. The level of fertility of the waters of Lake Toba in terms of the abundance of phytoplankton is included in the fertile category. If it is categorized at the level of pollution through the diversity index, then the waters are included in the moderately polluted category.
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Zhang, Chen Chen, Xiao Yun Liu, and Zhen Yu Wang. "Toxicity of Copper Oxide Nanoparticles on the Green Algae: Chlorella pyrenoidosa." Applied Mechanics and Materials 328 (June 2013): 758–62. http://dx.doi.org/10.4028/www.scientific.net/amm.328.758.

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The toxicity of CuO engineered nanoparticles (ENPs) to chlorella pyrenoidosa was investigated in this study. CuO ENPs inhibited the growth of algal cells significantly, while the Cu ion and CuO bulk particles (BPs) showed lower toxicity than ENPs. The black dots observed in cells using Transmission electron microscopic (TEM) were determined as copper compound by EDS analysis. Cu content in algal cells measured confirmed the Cu internalized.
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Mentari, Fentika Rahma, Boedi Hendrarto, and Haeruddin Haeruddin. "EFEK DARI PEWARNA BATIK BERBAHAN DASAR SAMPAHMANGROVE (Rhizophora sp.): UJI TOKSISITAS TERHADAP IKAN GUPPY(Poecilia reticulata) DAN Chlorella pyrenoidosa." Management of Aquatic Resources Journal (MAQUARES) 5, no. 4 (December 20, 2016): 190–98. http://dx.doi.org/10.14710/marj.v5i4.14407.

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ABSTRAK Sampah mangrove (Rhizophora sp.)telah dimanfaatkan sebagai pewarna alami untuk batik. Pemanfaatan ini diduga akan menghasilkan limbah cair yang dibuang langsung ke lingkungan perairan yang dapat menyebabkan pencemaran perairan dan meracuni biota air. Oleh karena itu diadakan penelitian untuk mengetahui efek pembuangan sisa pewarnaan batik dari limbah mangrove (Rhizhophora sp.) terhadap ikan dan alga. Metode penelitian yang digunakanadalah eksperimental skala laboratoris dengan menggunakan ikan guppy (P. reticulata) dan alga C. pyrenoidosa menyertakan uji toksisitas. Analisis data menggunakan analisis probit dengan persamaan regresi untuk mendapatkan nilai (LC50) 96 jam dan (IC50) 96 jam. Pertumbuhan ikan guppy dianalisa menggunakan analisis ANOVA dua faktor rancangan blok random lengkap dan data pertumbuhan alga dianalisa menggunakanANOVA petak terpisah. Data pertumbuhan ikan dan alga dianalisa dengan uji lanjut Dunnet untuk mengetahui nilai LOEC (Lowest-Observed Effect Concentration).Hasil yang diperoleh adalah nilai konsentrasi (LC50) 96 jam pada limbah pewarna batik mangrove adalah 29% dan diketahui mampu memberikan pengaruh nyata dalam menghambat laju pertumbuhan berat pada hewan uji ikan guppy (P < 0,05) dan mampu menghambat laju pertumbuhan Chlorella sp. dengan nilai konsentrasi penghambatan media (IC50) 96 jam adalah61,70%. Uji anova split plot in time pada konsentrasi 1,5625% memberikan pengaruh nyata (P < 0,05) pada jam ke-48 dalam pertumbuhan C. pyrenoidosa dengan nilai konsentrasi LOEC adalah 1,5625%. Kata kunci: Toksisitas;Rhizopora sp.; Ikan Guppy;Chlorella sp. ABSTRACT Mangrove litters (Rhizophora sp.) has been used as a natural dye for batik. This utilization is expected to produce liquid waste which is dumped directly into the aquatic environments which may cause water pollution and poisoning aquatic organism. Therefore it was necessary to do the research to determine the effect of batik dye from mangrove (Rhizophora sp.) against fish and algae. The methods used in this research was experimental laboratory scale using guppies (P. reticulata) and algae C. pyrenoidosa including toxicity test. The analysis of data used probit analysis with regression equations to get the value of (LC50) 96 hours and (IC50) 96 hours. Guppy fish growth data were analyzed using a complete random block design two way ANOVA and data of alga growth were analyzed with split plot analysis of variance. Fish and algae growth data were analyzed by a further test to determine the value of LOEC (Lowest-Observed Effect Concentration). Results obtained concentration values (LC50) 96 hours on mangrove batik dye waste was 29% and it was known to give significant effect in inhibiting the growth rate of the weight in the guppies animals test (P < 0,05), and also was able to inhibit the growth rate of C. pyrenoidosa with a medium inhibitory concentration values (IC50) 96 hours are61,70%. The test of split plot in time anova on the concentration of 1,5625% was giving significant effect (P < 0,05) at the 48th hour in the growth of C. pyrenoidosa with the concentration of LOEC was 1,5625%. Keywords: Toxicity;Rhizoporasp.; Poecilia reticulata; Chlorella sp.
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Huang, Jianke, Chao Zhang, Han Zhang, Ting Yao, Yi Du, Zheng Cheng, Ai-Hua Zhang, Daofeng Zhang, and Zhen Zhang. "Simultaneous Treatment of Swine and Furfural Wastewater Integrated with Lipid Production of Chlorella pyrenoidosa." Applied Sciences 12, no. 16 (August 14, 2022): 8144. http://dx.doi.org/10.3390/app12168144.

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Adding organic compounds to wastewater can improve the carbon/nitrogen ratio and benefit microalgae growth. We studied microalgal growth, nutrient removal and lipid accumulation of Chlorella pyrenoidosa cultured in a mixture of swine wastewater (SW) and furfural wastewater (FW). The mix ratio of SW:DFW (diluted furfural wastewater) had a significant effect on microalgae growth. As the mix ratio of SW:DFW decreased from 1:0.5 to 1:19, the maximum microalgal biomass increased, while the specific growth rate initially increased and then decreased. The efficiency of nutrient removal also depended on the mix ratio of wastewater. The highest chemical oxygen demand (COD) removal efficiency (57.30%) occurred at the mix ratio of SW:DFW = 1:3. The highest removal efficiencies of total phosphorous (TP) reached 61.93% when the mix ratio of SW:DFW was 1:9. Wastewater at the mix ratio of SW:DFW = 1:19 had a maximum lipid productivity of 49.48 mg L−1 d−1, which was 4.9 times higher than that at a mix ratio of SW:DFW = 1:0.5. These results showed that C. pyrenoidosa can be used to remove nutrients from mixed wastewater sources and simultaneously produce algal lipids.
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Zhuang, Xiuyuan, Daojing Zhang, Wen Qin, Jia Deng, Hui Shan, Liming Tao, and Yuanguang Li. "A comparison on the preparation of hot water extracts from Chlorella pyrenoidosa (CPEs) and radical scavenging and macrophage activation effects of CPEs." Food Funct. 5, no. 12 (2014): 3252–60. http://dx.doi.org/10.1039/c4fo00214h.

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Chen, Zhijie, and Bosheng Su. "Influence of Medium Frequency Light/Dark Cycles on the Cultivation of Auxenochlorella pyrenoidosa." Applied Sciences 10, no. 15 (July 24, 2020): 5093. http://dx.doi.org/10.3390/app10155093.

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Light (wavelength, intensity, and light/dark cycle) have been considered as one of the most important parameters for microalgae cultivation. In this paper, the effect of medium frequency intermittent light on Auxenochlorella pyrenoidosa (formerly Chlorella pyrenoidosa) cultivation was investigated. Three parameters of intermittent light, light intensity, light/dark ratio, and light/dark cycle were employed and the influence of these parameters on the productivity of Auxenochlorella pyrenoidosa was studied. The biomass yield and growth rates were mainly affected by the light fraction and cycle time. Light with 220 μE m−2 s−1 light intensity was determined as the optimal light intensity for biomass production. At the light intensity of 420 μE m−2 s−1, the results indicated that the intermittent light improved the biomass production with larger light/dark ratio compared with the continuous light. At a lower mean light intensity over time, the intermittent light should be more suitable for biomass growth and the decrease in the light/dark ratio (L/D) will lead to a higher biomass productivity. The light/dark cycle time has little influence on the biomass yield.
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Christian, Onwurah, Shahana Majumder, and Pankaj Taneja. "Growth Characteristics of Indian and Nigerian Chlorella Pyrenoidosa used as food Supplement." Biosciences, Biotechnology Research Asia 14, no. 2 (June 25, 2017): 835–41. http://dx.doi.org/10.13005/bbra/2516.

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Noorhana. "Effect of Nitrogen on Growth and Lipid Content of Chlorella pyrenoidosa." American Journal of Biochemistry and Biotechnology 7, no. 3 (September 1, 2011): 124–29. http://dx.doi.org/10.3844/ajbbsp.2011.124.129.

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Nigam. "Effect of Nitrogen on Growth and Lipid Content of Chlorella pyrenoidosa." American Journal of Biochemistry and Biotechnology 7, no. 3 (March 1, 2011): 126–31. http://dx.doi.org/10.3844/ajbbsp.2011.126.131.

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Chelladurai, Chellamboli, and Muthiah Perumalsamy. "Statistical Study on the Growth of Chlorella pyrenoidosa Using the Response Surface Methodology." Current Biochemical Engineering 4, no. 1 (February 3, 2017): 43–59. http://dx.doi.org/10.2174/1570180813666160527100108.

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Zhang, Yurui, Zixu Chen, Yue Tao, Wanyin Wu, Yuyang Zeng, Kejun Liao, Xinyue Li, and Lanzhou Chen. "Transcriptomic and Physiological Responses of Chlorella pyrenoidosa during Exposure to 17α-Ethinylestradiol." International Journal of Molecular Sciences 23, no. 7 (March 25, 2022): 3583. http://dx.doi.org/10.3390/ijms23073583.

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17α-ethinylestradiol (17α-EE2) is frequently detected in water bodies due to its use being widespread in the treatment of prostate and breast cancer and in the control of alopecia, posing a threat to humans and aquatic organisms. However, studies on its toxicity to Chlorella pyrenoidosa have been limited to date. This study investigated the effects of 17α-EE2 on the growth, photosynthetic activity, and antioxidant system of C. pyrenoidosa and revealed related molecular changes using transcriptomic analysis. The cell density of algae was inhibited in the presence of 17α-EE2, and cell morphology was also altered. Photosynthetics were damaged, while reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA) content increased. Further transcriptomic analysis revealed that the pathways of photosynthesis and DNA replication were affected at three concentrations of 17α-EE2, but several specific pathways exhibited various behaviors at different concentrations. Significant changes in differentially expressed genes and their enrichment pathways showed that the low-concentration group was predominantly impaired in photosynthesis, while the higher-concentration groups were biased towards oxidative and DNA damage. This study provides a better understanding of the cellular and molecular variations of microalgae under 17α-EE2 exposure, contributing to the environmental risk assessment of such hazardous pollutants on aquatic organisms.
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Johar Gunawan, Teuku, Yusni Ikhwan, Fajar Restuhadi, and Usman Pato. "Effect of light Intensity and photoperiod on growth of Chlorella pyrenoidosa and CO2 Biofixation." E3S Web of Conferences 31 (2018): 03003. http://dx.doi.org/10.1051/e3sconf/20183103003.

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Microalgae have been viewed as one of potential solution for CO2 biofixation or CO2 sequestration. However, many factors need to be evaluated to support development of CO2 biofixation. One important environmental factor for the growth of micro algae is related with light requirement. The aim of this study was to evaluate the effect of light intensity and photoperiod on growth of Chlorellapyrenoidosa (C.pyrenoidosa) and CO2 biofixation. Experiments were carried out in 1000 mL semi batch photo bioreactors, purged continuously with air (0.034% CO2). An Experiment of Factorial Design was employed in which the light intensity was evaluated 4 level at 2000, 4000, 6000 and 8000 lux with 3 level of photo period at L/D (light /dark) 8 hours/16 hours; L/D 12 hours/12 hours and L/D 16 hours/8 hours. The result indicated that both light intensity and photo period had significant effect (p< 0.05) on growth of C. pyrenoidosa. However, the photo period showed stronger effect relative to light intensity on growth of C.pyrenoidosa within the range reviewed. The interaction between the two factors was indicative but statistically not significant. Best growth profile sustained at combination of L/D 16 hours/8 hours of photoperiod and light intensity of 8000 lux with the highest average biomass observed at 0.516 ± 0.069gr/L. An increase in CO2biofixation rate of around 2 times was also observed between highest setting (8000 lux; L/D 16/12 hours) relative to that of lowest setting (2000 lux; L/D 8/12 hours).
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Feng, Yu, Ao Wang, Wenxian Fu, and Donghui Song. "Growth performance, antioxidant response, biodegradation and transcriptome analysis of Chlorella pyrenoidosa after nonylphenol exposure." Science of The Total Environment 806 (February 2022): 150507. http://dx.doi.org/10.1016/j.scitotenv.2021.150507.

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GONZALEZ-MURUA, C., A. MUNOZ-RUEDA, F. HERNANDO and, and M. SANCHEZ-DIAZ. "Effect of atrazine and methabenzthiazuron on oxygen evolution and cell growth of Chlorella pyrenoidosa." Weed Research 25, no. 1 (February 1985): 61–66. http://dx.doi.org/10.1111/j.1365-3180.1985.tb00618.x.

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Sachdeva, Neha, G. Dinesh Kumar, Ravi Prakash Gupta, Anshu Shankar Mathur, B. Manikandan, Biswajit Basu, and Deepak Kumar Tuli. "Kinetic modeling of growth and lipid body induction in Chlorella pyrenoidosa under heterotrophic conditions." Bioresource Technology 218 (October 2016): 934–43. http://dx.doi.org/10.1016/j.biortech.2016.07.063.

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Lee, Yuan-Kun, and Hong-Soon Tay. "High CO2 partial pressure depresses productivity and bioenergetic growth yield of Chlorella pyrenoidosa culture." Journal of Applied Phycology 3, no. 2 (June 1991): 95–101. http://dx.doi.org/10.1007/bf00003690.

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Wu, Penghui, Lei Zhang, Haixing Chang, Guoli Xu, and Mengxue Liu. "Investigation of hydrogen peroxide-driven transcriptional stress on the biomass growth of Chlorella pyrenoidosa." Algal Research 68 (November 2022): 102897. http://dx.doi.org/10.1016/j.algal.2022.102897.

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Zhong, Xueqing, Yali Zhu, Yujiao Wang, Quanyu Zhao, and He Huang. "Effects of three antibiotics on growth and antioxidant response of Chlorella pyrenoidosa and Anabaena cylindrica." Ecotoxicology and Environmental Safety 211 (March 2021): 111954. http://dx.doi.org/10.1016/j.ecoenv.2021.111954.

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Martínez, Ma E., F. Camacho, J. M. Jiménez, and J. B. Espínola. "Influence of light intensity on the kinetic and yield parameters of Chlorella pyrenoidosa mixotrophic growth." Process Biochemistry 32, no. 2 (February 1997): 93–98. http://dx.doi.org/10.1016/s0032-9592(96)00045-3.

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Fan, Jianhua, Yanbin Cui, Yang Zhou, Minxi Wan, Weiliang Wang, Jingli Xie, and Yuanguang Li. "The effect of nutrition pattern alteration on Chlorella pyrenoidosa growth, lipid biosynthesis-related gene transcription." Bioresource Technology 164 (July 2014): 214–20. http://dx.doi.org/10.1016/j.biortech.2014.04.087.

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Zhang, Weiguo, Peiliang Zhang, Hao Sun, Maozhen Chen, Shan Lu, and Pengfu Li. "Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa." Bioresource Technology 173 (December 2014): 52–58. http://dx.doi.org/10.1016/j.biortech.2014.09.084.

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Wang, Qing, Wenbiao Jin, Xu Zhou, Shida Guo, Shu-Hong Gao, Chuan Chen, Renjie Tu, Song-Fang Han, Jiao Jiang, and Xiaochi Feng. "Growth enhancement of biodiesel-promising microalga Chlorella pyrenoidosa in municipal wastewater by polyphosphate-accumulating organisms." Journal of Cleaner Production 240 (December 2019): 118148. http://dx.doi.org/10.1016/j.jclepro.2019.118148.

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Kokkiligadda, Sujana, Bindiya Pandey, and Srinivasa Reddy Ronda. "Effect of plant growth regulators on production of alpha-linolenic acid from microalgae Chlorella pyrenoidosa." Sādhanā 42, no. 10 (October 2017): 1821–24. http://dx.doi.org/10.1007/s12046-017-0723-8.

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Wang, Xiucui, Xuezhu Zhu, Xuemei Chen, Baitao Lv, Xue Wang, and Danqin Wang. "Phenanthrene and pyrene disturbed the growth of Microcystis aeruginosa as co-cultured with Chlorella pyrenoidosa." Environmental Science and Pollution Research 27, no. 36 (October 17, 2020): 45957–64. http://dx.doi.org/10.1007/s11356-020-10979-7.

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Yu, Zongyi, Lu Liu, Junhui Chen, Xinpeng Wang, and Dong Wei. "Effect of crude glycerol on heterotrophic growth of Chlorella pyrenoidosa and Coccomyxa subellipsoidea C-169." Journal of Applied Phycology 30, no. 6 (June 23, 2018): 2989–96. http://dx.doi.org/10.1007/s10811-018-1551-x.

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