Relevant bibliographies by topics / Chinese family life

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Books
  4. Book chapters
  5. Conference papers

Academic literature on the topic 'Chinese family life'

Author: Grafiati
Published: 4 June 2021
Last updated: 19 February 2022

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Journal articles on the topic "Chinese family life"

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Whyte, Martin King. "Continuity and Change in Urban Chinese Family Life." China Journal 53 (January 2005): 9–33. http://dx.doi.org/10.2307/20065990.

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Ren, Xiaoni, and Darren John Caudle. "Balancing academia and family life." Gender in Management: An International Journal 35, no. 2 (February 20, 2020): 141–65. http://dx.doi.org/10.1108/gm-06-2019-0093.

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Purpose This paper aims to explore and compare academics’ experiences of managing work-life balance (WLB) in the British and Chinese contexts. The authors have three specific purposes. Firstly, to investigate whether there are marked gender differences in either context, given female and male academics’ work is considered fully comparable. Secondly, to examine contextual factors contributing to gender differences that influence and shape decisions in WLB and career paths. Thirdly, to explore the gendered consequences and implications. Design/methodology/approach A cross-national and multilevel analytical approach to WLB was chosen to unpick and explore gender land contextual differences and their influence on individual academics’ coping strategies. To reflect the exploratory nature of uncovering individual experience and perceptions, the authors used in-depth, semi-structured interviews. In total, 37 academics participated in the study, comprised of 18 participants from 6 universities in the UK and 19 participants from 6 universities in China. Findings This study reveals gendered differences in both the British and Chinese contexts in three main aspects, namely, sourcing support; managing emotions; and making choices, but more distinct differences in the latter context. Most significantly, it highlights that individual academics’ capacity in cultivating and using coping strategies was shaped simultaneously by multi-layered factors at the country level, the HE institutional level and the individual academics’ level. Originality/value Very few cross-cultural WLB studies explore gender differences. This cross-national comparative study is of particular value in making the “invisible visible” in terms of the gendered nature of choices and decisions within the context of WLB. The study has significant implications for female academics exercising individual scope in carving out a career, and for academic managers and institutions, in terms of support, structure and policy.
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CHAO, Fanglin. "Elderly’s Family Life Supplies - Innovative Chinese Checkers Game Board." IOP Conference Series: Materials Science and Engineering 234 (September 2017): 012024. http://dx.doi.org/10.1088/1757-899x/234/1/012024.

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Zhao, Yang, Yuka Fujimoto, and Sanjaya Singh Gaur. "Life enrichment for workers in contemporary China." Journal of Organizational Change Management 31, no. 1 (February 12, 2018): 173–87. http://dx.doi.org/10.1108/jocm-06-2017-0239.

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Purpose The purpose of this paper is to identify key antecedents of work-family enrichment (WFE) for Chinese workers in China. The paper adopts the Chinese cultural perspectives (i.e. philosophy of Confucian: Chinese family orientation and collectivism) as well as traditional Chinese philosophies of life as a whole. Design/methodology/approach This is a conceptual paper. Therefore, this section does not apply. Findings The key antecedents of Chinese WFE have been identified and presented in the conceptual framework. Testable propositions have also been developed and presented in this paper. Practical implications The conceptual framework showing the identified key antecedents of Chinese WFE highlights the necessity for the corporate leaders to rethink the ways to promote well-being and productivity of Chinese workforce in China. Meanwhile, managers should rethink about WFE among Chinese employees and regard employees as a whole person rather than just a worker with certain skills or abilities. Originality/value This is the first paper to propose the concept of life enrichment by examining the antecedents of WFE particularly from the Chinese philosophical and cultural perspective.
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Wang, Kaipeng, Yanqin Liu, Fei Sun, Dexia Kong, and Bei Wu. "Attitude Toward Family Involvement in End-of-Life Care Among Older Chinese Americans." Innovation in Aging 4, Supplement_1 (December 1, 2020): 507. http://dx.doi.org/10.1093/geroni/igaa057.1638.

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Abstract Family involvement is critical to end-of-life (EOL) care of older adults. Attitude toward family involvement in EOL care can be influenced by family relationship. Yet, mechanisms explaining such influence have not been examined among older Chinese Americans. This study aims to examine the association between family relationship and older Chinese Americans’ attitude toward family involvement in EOL care and explore pathways of this association. Potential mediators include self-efficacy, perceived benefits, and perceived barriers of discussing EOL care with family members. Data were collected from 276 Chinese Americans aged 55+ in two metropolitan areas in 2018. Participants’ average age was 74 years (SD=9.6). Approximately 64% of the sample were female. Most participants (57%) held positive attitudes toward family involvement in EOL care. Using the Structural Equation Modeling method, we found that family relationship had a significant positive total effect on positive attitude toward family involvement in EOL care (z=5.57, p<0.001). Indirect direct of family relationship on attitude toward family involvement in EOL care through both self-efficacy (z=3.13, p<0.01) and perceived barriers (z=2.30, p<0.05) of discussing EOL care with family members was significant. Results suggest that improving family relationship may increase elder’s self-efficacy and reduce barriers of discussing EOL with family members, which is associated with more positive attitude toward family involvement in EOL care. Findings provide empirical evidence of how family relationship affects older Chinese Americans’ attitude toward family involvement in EOL care and underline the need for family-centered interventions for older Chinese Americans.
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Ngan, Raymond, and William Wong. "Injustice in Family Care of the Chinese Elderly in Hong Kong." Journal of Aging & Social Policy 7, no. 2 (January 29, 1996): 77–94. http://dx.doi.org/10.1300/j031v07n02_06.

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Ownby, David, and Frank Ching. "Ancestors: 900 Years in the Life of a Chinese Family." Pacific Affairs 62, no. 3 (1989): 390. http://dx.doi.org/10.2307/2760634.

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Wang, W., and T. McDonald. "Patient, family, nurse perspectives on Chinese elders' quality of life." International Nursing Review 64, no. 3 (November 11, 2016): 388–95. http://dx.doi.org/10.1111/inr.12333.

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Choi, Jaepil, and Chao C. Chen. "Gender Differences in Perceived Work Demands, Family Demands, and Life Stress among Married Chinese Employees." Management and Organization Review 2, no. 2 (July 2006): 209–29. http://dx.doi.org/10.1111/j.1740-8784.2006.00041.x.

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Although gender-based division of labour and the identity theory of stress suggest that the relationship between work and family demands and life stress may vary as a function of gender, it is largely unknown whether these arguments are also valid in China. To address this gap in the existing literature, the current study investigates the gender differences in perceived work and family demands, and the effects of these perceived demands on the life stress of Chinese male and female employees. The study of 153 married Chinese employees found that Chinese women perceived a higher level of family demands than did Chinese men, whereas there was no significant gender difference in the perception of work demands. In addition, while perceived family demands were similarly related to life stress differently for men and women, perceived work demands were associated more strongly with the life stress of men than that of women.
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Pei, Yaolin, Wei Zhang, and Bei Wu. "Advance Care Planning Engagement and End-of-Life Preference Among Older Chinese Americans." Innovation in Aging 4, Supplement_1 (December 1, 2020): 750–51. http://dx.doi.org/10.1093/geroni/igaa057.2704.

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Abstract The study aimed to examine how immigrant status and family relationships are associated with advanced care planning (ACP) engagement and end-of-life (EOL) preference over burial plan among older Chinese Americans, the largest subgroup of Asian Americans. Logistic regressions were used to analyze data from a survey of 430 older Chinese Americans aged 55 and older living in a Honolulu, Hawai’i. Results show that U.S.-born Chinese Americans were more likely to engage in ACP, including willingness thought of EOL care, and discussion about EOL care, and having preference over burial plan, than the foreign-born Chinese American. Family cohesion was not associated with ACP engagement and EOL preference over burial plan. Family conflict increased the possibility of ACP engagement, indicated by willingness thought of ACP, willingness discussion on ACP, and EOL preference over burial plan. The culturally sensitive educational intervention is needed to increase ACP engagement among older Chinese Americans.
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Dissertations / Theses on the topic "Chinese family life"

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Yang, Hongyi. "Herb and Life: A Chinese Medical Family." Thesis, University of North Texas, 2002. https://digital.library.unt.edu/ark:/67531/metadc3296/.

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This written thesis examines the process of producing Herb and Life: a Chinese Medical Family, a thirty-minute documentary video that explores the producer's family members' relationship with Traditional Chinese Medicine. This documentary uses interviews, narration, music, and observational sequences to display documentary subjects' career choices and their experiences with Traditional Chinese Medicine. This written thesis reveals the development of this documentary, from the pre-production to production and post-production stages. It also incorporates theoretical analysis and self-evaluation of this documentary video.
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Fung, Gordon Lindsay. "Filial piety with Chinese family teachings." online access from Digital Dissertation Consortium, 2006. http://libweb.cityu.edu.hk/cgi-bin/er/db/ddcdiss.pl?3264342.

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Chen, Frederic. "How to develop a strong family ministry in North American Chinese churches scriptural principles for family living /." Theological Research Exchange Network (TREN), 1988. http://www.tren.com.

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Lee, Joyce Wai Kuan. "Cancer and the family : distress and quality of life among Chinese-speaking patients and family caregivers." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/59419.

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Background: Cancer is a family disease affecting the patient and family members. To date, few studies have explored the psychological distress of family caregivers and its relation with patient quality of life (QOL), particularly among culturally diverse cancer populations. This study seeks to understand the associations between patient distress, family caregiver distress and patient QOL in a Chinese-speaking population in British Columbia, Canada. Methods: A cross-sectional survey of Chinese-speaking (study population) and Anglophone (comparison group) patients (N = 55) and their family caregivers (N = 40) was used to examine patient QOL and correlates. Multiple imputation of missing data for incomplete dyads resulted in data for 29 Chinese-speaking and 28 Anglophone dyads. Multiple linear regression and mediation analyses examined predictors of QOL, and its domains, and mediation effects of patient distress and family caregiver distress. Semi-structured interviews with a subset of the Chinese-speaking survey participants comprising ten patients and six family caregivers, including five patient-family caregiver dyads, were also conducted. Analysis of culturally-embedded experiences of patients and family caregivers regarding their distress and QOL was conducted, to provide further context and explanation for the findings from the quantitative analysis. Results: Patient distress emerged as the key factor in explaining patient QOL. The effects of patient age on patient emotional well-being were mediated by patient distress, such that lower distress in older patients explained better emotional functioning. A key theme that surfaced from the qualitative data analysis was the emotional regulation occurring in the family when coping with cancer. The negative impact of family caregiver distress on patient emotional well-being was acknowledged by both the patients and the family caregivers. Younger and older patient interviewees alike indicated anxiety and worries about the impact of their cancer diagnosis on their families. Conclusions: This study provides insights to Chinese-speaking patients’ and family caregivers’ illness experience and the interrelatedness of the dyads’ responses to cancer. In expanding our knowledge of cross-cultural cancer care, these findings highlight the important role of cultural background in shaping service needs and, in turn, service delivery to cancer patients and their families.
Medicine, Faculty of
Population and Public Health (SPPH), School of
Graduate
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Mai, Dan T. "Sustaining family life in rural China : reinterpreting filial piety in migrant Chinese families." Thesis, University of Oxford, 2015. http://ora.ox.ac.uk/objects/uuid:8e679650-a857-4f3c-a5c1-770a1bff848e.

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This study explores the changing nature of filial piety in contemporary society in rural China. With the economic, social and political upheavals that followed the Revolution, can 'great peace under heaven' still be found for the rural Chinese family as in the traditional Confucian proverb,"make yourself useful, look after your family, look after your country, and all is peaceful under heaven"? This study explores this question, in terms not so much of financial prosperity, but of non-tangible cultural values of filial piety, changing familial and gender roles, and economic migration. In particular, it examines how macro level changes in economic, social and demographic policies have affected family life in rural China. The primary policies examined were collectivisation, the hukou registration system, marketization, and the One-Child policy. Ethnographic interviews reveal how migration has affected rural family structures beyond the usual quantifiable economic measures. Using the village of Meijia, Sichuan province, as a paradigmatic sample of family, where members have moved to work in the cities, leaving their children behind with the grandparents, the study demonstrates how migration and modernization are reshaping familial roles, changing filial expectations, reshuffling notions of care-taking, and transforming traditional views on the value of daughters and daughters-in-law. The study concludes that the choices families make around migration, child-rearing and elder-care cannot be fully explained by either an income diversification model or a survival model, but rather through notions of filial piety. Yet the concept of filial piety itself is changing, particularly in relation to gender and perceptions about the worth of daughters and the mother/ daughter-in-law relationship. Understanding these new family dynamics will be important for both policy planners and economic analysts.
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Chan, Kenneth, and n/a. "Chinese history books and other stories." University of Canberra. Creative Communication, 2005. http://erl.canberra.edu.au./public/adt-AUC20061020.144139.

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My thesis is a creative writing doctorate which focuses on one Chinese family's adaptation to living in Australia in the mid-twentieth century. The thesis is in two parts. Part I is an examination of Chineseness and identity within the context of the short stories that make up Part I1 of the thesis. In Part I, I have looked at the place of the Chinese within the larger, dominant cultures of America and Australia. In particular, I have discussed the way in which the discourses of the dominant culture have framed Chineseness; and also what it might mean to describe authentic and essential qualities in Chineseness. The question I ask is whether the concept of Chineseness shifts according to time, location, history, and intercultural encounters. This leads me to try to "place" my family and myself. I provide some background on my family and on specific incidents that have served as springboards for the fiction. Part I also discusses some aspects of narrative theory in relation to the stories and considers the stories within the context of other Chinese- Australian fiction and performance. Ln Part 11, I have written a collection of nine short stories about the lives of a fictitious family called the Tangs. The stories can be described as a cycle that is unified and linked by characters who are protagonists in one story but appear in a minor or supporting role in other stories. Composing a linked cycle of stories has given me the opportunity to extend the short story form, especially by giving me scope to expand the lives of the characters beyond a single story. The lives of the characters can take on greater complexity since they confront challenges at different stages of their lives from different perspectives.
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Sham, Sylvia. "Cultural differences in teaching and learning styles : a case study of Chinese adolescents." Thesis, Manchester Metropolitan University, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336492.

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Chung, Lai-ping. "A study of the family life adaptation of new immigrant wives from China /." Hong Kong : University of Hong Kong, 1996. http://sunzi.lib.hku.hk/hkuto/record.jsp?B19470083.

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Wu, Esther Lo. "The Chinese view of the ideal family life as seen in the light of the Bible." Lynchburg, Va. : Liberty University, 1991. http://digitalcommons.liberty.edu.

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Wu, Yikun. "Stress, satisfaction and resilience : the psychological aspects of life for elders in a Chinese setting." Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/942/.

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Little research to date has focused on personal accounts of psychological aspects of the aging process of elders and their relationship with adult-children in an urban Chinese setting. The present study aimed to explore such aspects in Chinese elders. Semi-structured interviews were conducted with 3 varied samples of elders (aged 70 to 87), with those living with their family (study 1), those in a residential setting (study 2) and those in a sheltered community (study 3). Grounded Theory, was used as the method for data collection and analysis, and in addition to the interviews, focus groups and a written account also provided data. The overarching theme of “family communication” was highlighted in the studies, reflecting the central position of participants’ concerns with their relationship with adult-children, and their understanding of their roles in the family. The variety of responses seemed to be connected with whether the person was self-reliant or dependant as well as with individual personality. The study’s findings suggest that this small sample of older people in a large urban city in China generally live closely with their families and take great pride in retaining their independence and supporting families and friends.
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Books on the topic "Chinese family life"

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Chan, Sui-Lin Lisa. Wife assault: The Chinese Family Life Services experience. Toronto: Chinese Family Life Services of Metro Toronto, 1989.

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Jung, John. Sweet and sour: Life in Chinese family restaurants. [Cypress, Calif.]: Yin and Yang Press, 2010.

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Jung, John. Sweet and sour: Life in Chinese family restaurants. [Cypress, Calif.]: Yin and Yang Press, 2010.

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Yolen, Jane. And twelve Chinese acrobats. New York: Philomel Books, 1994.

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Yolen, Jane. And twelve Chinese acrobats. New York: Philomel Books, 1994.

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Yolen, Jane. And twelve Chinese acrobats. New York: Philomel Books, 1994.

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Ba, Jin. The family: A contemporary Chinese classic. Singapore: ASIAPAC Books, 1995.

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Yan, Wong San, ed. My grandmother's Chinese kitchen: 100 family recipes and life lessons. New York, N.Y: Home, 2006.

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Ching, Frank. Ancestors: 900 years in the life of a Chinese family. London: Harrap, 1988.

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Ching, Frank. Ancestors: 900 years in the life of a Chinese family. London: Pan Books, 1989.

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Book chapters on the topic "Chinese family life"

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Shek, Daniel T. L. "Chinese Family Quality of Life." In Encyclopedia of Quality of Life and Well-Being Research, 842–47. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-0753-5_355.

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Yeung, Jerf W. K. "Linked life." In Religion, Family, and Chinese Youth Development, 148–212. Abingdon, Oxon ; New York, NY : Routledge, 2021. |: Routledge, 2020. http://dx.doi.org/10.4324/9780429284885-6.

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Shek, Daniel T. L., and Rachel C. F. Sun. "Chinese Family Assessment Instrument." In Encyclopedia of Quality of Life and Well-Being Research, 839–42. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-0753-5_3553.

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Shek, Daniel T. L., and Lu Yu. "Chinese Version of Family Assessment Device." In Encyclopedia of Quality of Life and Well-Being Research, 885–87. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-0753-5_3552.

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Shek, Daniel T. L. "Chinese Version of Self-Report Family Inventory." In Encyclopedia of Quality of Life and Well-Being Research, 887–90. Dordrecht: Springer Netherlands, 2014. http://dx.doi.org/10.1007/978-94-007-0753-5_3551.

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ChenFeng, Jessica. "Bridging the Relational Space Between First- and Second-Generation Chinese American Christians." In Transition and Change in Collectivist Family Life, 33–43. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-50679-1_4.

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Um, Seong-gee. "Struggling to Make Time for Family: Work and Family Life of Korean-Chinese Institutional Care Workers in South Korea." In Migrant Domestic Workers and Family Life, 257–75. London: Palgrave Macmillan UK, 2015. http://dx.doi.org/10.1057/9781137323552_13.

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Chen, Hao-Min, and Denise C. Lewis. "The Role of Chinese Grandparents in Their Adult Children’s Parenting Practices in the United States." In Transition and Change in Collectivist Family Life, 57–66. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-50679-1_6.

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Shek, Daniel T. L., and Rachel C. F. Sun. "Promotion of Positive Youth Development and Family Quality of Life in Chinese Adolescents." In Quality of Life in Asia, 221–37. Singapore: Springer Singapore, 2014. http://dx.doi.org/10.1007/978-981-287-143-5_12.

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Shih, Kristy Y. "Addressing Power and Resistance with Chinese American Daughters-in-Law and Their Immigrant Mothers-in-Law." In Transition and Change in Collectivist Family Life, 11–20. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-50679-1_2.

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Conference papers on the topic "Chinese family life"

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Luan, Xiaoying, and Yi Zhang. "A study on the mode of public participation in Chinese urban design under the concept of multi-body participation. Community building oriented with multi-age participation." In Post-Oil City Planning for Urban Green Deals Virtual Congress. ISOCARP, 2020. http://dx.doi.org/10.47472/gxie2200.

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With the society and economy of China entering the transition period, people's requirements for urban life show a trend of diversification and high standards. Urban design is no longer limited to physical space but starts to carry out multi-angle and multi-level comprehensive consideration on the social and spatial problems of symbiosis. In this context, the innovative social governance and the quality improvement of urban public space that highlights the role of the public have become hot topics. As the main body of city users, ordinary people entering into the deep and substantial participation from the superficial and symbolic is the only way for urban design to transform from the expert-led "optimal scheme" to the "reasonable scheme" recognized by pluralistic bodies. In the process of western democratic politics, the participation theory that focuses on the construction of micro democracy is prevalent, and the public participation based on pluralism has become the theoretical basis for the planning participation. Besides, the concept of communicative planning and collaborative planning, which emphasize cooperation and consultation, also enriches the connotation of multiple participation. Due to different systems and awareness, it is difficult to promote public participation under China's national conditions. Community building, the interdisciplinary product of urban design and social governance under communities, is regarded as the experimental field for participating in the reform. Therefore, under the current administrative system and regulations, this paper tries to make use of the grass-roots management mode with Chinese characteristics to establish an inclusive multi-participation mechanism. It allows residents of different ages can go deep into the process of community building by taking the family unit. Meanwhile, some suggestions and strategies are raised for effective participation. We hope the bottom-up process of urban design in microscale can be an effective instrument to reflect the public's will and repair social relations at the same time, while explore and solve urban problems in diverse cooperation.
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Popovici, Violeta, Laura Bucur, Gabriela Vochita, Victoria Badea, and Florin-Ciprian Badea. "CONTRIBUTIONS TO THE COMPLEX STUDY ON ANTITUMOR ACTIVITY OF USNEA BARBATA (L.) F.H.WIGG." In NORDSCI International Conference. SAIMA Consult Ltd, 2020. http://dx.doi.org/10.32008/nordsci2020/b1/v3/25.

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Usnea barbata (L.)F.H.Wigg. - known as “old man’s beard”, “tree moss”, “songluo” is a lichen in the family Parmeliaceae, genus Usnea. Usnea species have recorded history of therapeutic use dating back over three thousand years in Chinese medicine. The lichen secondary metabolites have shown an impressive range of biological proprieties, including antibiotic, antifungal, antiviral, anti-inflammatory, or anticancer activities. In this study, the antitumor activity of Usnea barbata extract was evaluated by observing the morphological changes on squamous cells carcinoma cell-line CAL 27 (ATCC® CRL-2095 ™) in contact with different concentrations of extract, ranged between 12.5–400 μg/mL. The results obtained were quantified by the intensity of morphological changes of the tumor cells after 24 hours of contact. The most significant activity were recorded for 400 μg/mL extract. This study shows that Usnea barbata (L.)F.H.Wigg. extract has antitumor activity. The analysis of the obtained results showed that the cytotoxicity of lichen extract on CAL 27 tumor cells is directly related to the concentration of the applied solution.
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French, Jesse J., Caitlin T. Clancy, Allison L. Johnston, Maria A. Holland, and John M. Henshaw. "Design and Fabrication of the Energy Generating Components for the Sustainable Shepherd’s Residence in Northeastern China." In ASME 2009 3rd International Conference on Energy Sustainability collocated with the Heat Transfer and InterPACK09 Conferences. ASMEDC, 2009. http://dx.doi.org/10.1115/es2009-90074.

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The Sustainable Shepherd’s Residence (SSR) is a novel, multi-year service learning project executed by students at the University of Tulsa in the Jilin Province of Northeastern China. The SSR is an effort to design and fabricate a dwelling that is manufacturable with local materials on a budget commensurate with local earnings. It utilizes modern sustainable energy methods to bring power to some of the 20 million rural Chinese who live without electricity in one of the world’s harshest climates. Wind power, passive solar heating, photovoltaic battery charging, and biogas technology are all incorporated into a structure that houses a single family and their associated livestock. After completing an assessment trip to the region to inventory health conditions, energy needs, and available materials, students designed a residence that provided greatly improved living conditions for the rural shepherd whilst accommodating the special security issues that remain a challenge in the region. On subsequent return trips, the students worked alongside local craftsmen to build the structure and the sustainable energy components and assemble them into a ‘green model home’. The SSR is currently occupied and visited frequently by farmers from neighboring villages who seek to improve their standard of living using the sustainable techniques on display. This paper focuses on the challenges associated with the fabrication of fairly well understood modern sustainable energy technologies in the face of cultural and materiel difficulties present in an international and rural setting. The competing forces at play include the lack of basic maintenance habits due to long-term absence of privatized machinery ownership and the desire for high-wattage electrical conveniences found in the larger cities. The practical engineering aspects of the design and testing of the SSR energy generating components are considered. Specific topics include the fabrication and modification of two different wind turbines, manufacture and operational testing of a biogas digester, and the design of an integrated residential greenhouse with an aquaponics system capable of year round production.
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Homes, W. E., H. R. Lijnen, L. Nelles, C. Kluft, and D. Collen. "AN ALANINE INSERTION IN α2-ANTIPLASMIN ‘ENSCHEDE’ ABOLISHES ITS PLASM IN INHIBITORY ACTIVITY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642897.

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Congenital deficiencies of the fibrinolytic inhibitor α2antiplasmin (α2AP) may result in bleeding disorders. An abnormal a AP (α2AP‘Enschede’) is known. 2 siblings with 3% functional activity and normal antigen level have parents with 50% activity and normal antigen. The protein interacts normally with the lysine-binding site(s) of plasmin(ogen) but does not inhibit plasmin irreversibly. α2AP Enschede is a plasmin substrate that like the normal protein releases a M 8,000 peptide upon reaction with plasmin. In the present study, Southern blot analysis, using an α2AP cDNA probe showed a restriction fragment length polymorphism within a small genomic DNA fragment of the Enschede family members. Cloning and sequencing of these fragments revealed a GCG inframe insertion that results in an alanine addition between amino acids 353 and 357, 7-10 positions NH -terminal to the reactive site PI residue, Arg364. This area is homologous to the A4 B-sheet of reactive site cleaved a -antitrypsin. Clones from each individual confirm the parents as true heterozygotes and the children as true homozygotes. A cloned genomic DNA sequence containing the insertion (V ) was exchanged for the normal sequence in a eukaryotic a AP expression plasmid. Recombinant α2AP‘Enschede’ (ra AfVAla) purified from the conditioned media of transfected Chinese Hamster Ovary Cells is analogous to plasma a α2AP‘Enschede’ with respect to interactions with plasmin and plasminogen. Preliminary analysis of the released Mr 8,000 recombinant peptide shows that its NH -terminus is the same as the peptide cleaved from normal a AP. Although ra α2APVAla does not inhibit plasmin irreversibly it does, however, act as a competitive inhibitor of hydrolysis of the chromogenic substrate S-2251 by plasmin.The K for this interaction is 25 nM. Thus, α2APAla retains a high affinity for the active center of plasmin. In conclusion, an Ala insertion near the reactive site of α2AP must have resulted in a structural perturbation that has abolished the plasmin inhibitory activity of a α2AP‘Enschede’. This variant may provide a model for further investigation of structure-function relationships in the serpins which determine the relative inhibitor vs. substrate properties.
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