Journal articles: 'Chemosensory proteins'

1

Randazzo, B., F. Abbate, E. Ciriaco, G. Montalbano, M. F. Madrigrano, and M. B. Levanti. "Chemosensory proteins in the chemosensory organs of adult zebrafish." Annals of Anatomy - Anatomischer Anzeiger 207 (September 2016): 125. http://dx.doi.org/10.1016/j.aanat.2016.04.024.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Ban, L., A. Scaloni, A. Brandazza, S. Angeli, L. Zhang, Y. Yan, and Paolo Pelosi. "Chemosensory proteins of Locusta migratoria." Insect Molecular Biology 12, no. 2 (April 2003): 125–34. http://dx.doi.org/10.1046/j.1365-2583.2003.00394.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Rondoni, Gabriele, Alessandro Roman, Camille Meslin, Nicolas Montagné, Eric Conti, and Emmanuelle Jacquin-Joly. "Antennal Transcriptome Analysis and Identification of Candidate Chemosensory Genes of the Harlequin Ladybird Beetle, Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)." Insects 12, no. 3 (March 2, 2021): 209. http://dx.doi.org/10.3390/insects12030209.

Full text

Abstract:

In predatory ladybirds (Coleoptera: Coccinellidae), antennae are important for chemosensory reception used during food and mate location, and for finding a suitable oviposition habitat. Based on NextSeq 550 Illumina sequencing, we assembled the antennal transcriptome of mated Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae) males and females and described the first chemosensory gene repertoire expressed in this species. We annotated candidate chemosensory sequences encoding 26 odorant receptors (including the coreceptor, Orco), 17 gustatory receptors, 27 ionotropic receptors, 31 odorant-binding proteins, 12 chemosensory proteins, and 4 sensory neuron membrane proteins. Maximum-likelihood phylogenetic analyses allowed to assign candidate H. axyridis chemosensory genes to previously described groups in each of these families. Differential expression analysis between males and females revealed low variability between sexes, possibly reflecting the known absence of relevant sexual dimorphism in the structure of the antennae and in the distribution and abundance of the sensilla. However, we revealed significant differences in expression of three chemosensory genes, namely two male-biased odorant-binding proteins and one male-biased odorant receptor, suggesting their possible involvement in pheromone detection. Our data pave the way for improving the understanding of the molecular basis of chemosensory reception in Coccinellidae.

APA, Harvard, Vancouver, ISO, and other styles

4

Segura-León, Obdulia L., Brenda Torres-Huerta, Alan Rubén Estrada-Pérez, Juan Cibrián-Tovar, Fidel de la Cruz Hernandez-Hernandez, José Luis Cruz-Jaramillo, José Salvador Meza-Hernández, and Fabian Sánchez-Galicia. "Identification of Candidate Chemosensory Gene Families by Head Transcriptomes Analysis in the Mexican Fruit Fly, Anastrepha ludens Loew (Diptera: Tephritidae)." International Journal of Molecular Sciences 23, no. 18 (September 11, 2022): 10531. http://dx.doi.org/10.3390/ijms231810531.

Full text

Abstract:

Insect chemosensory systems, such as smell and taste, are mediated by chemosensory receptor and non-receptor protein families. In the last decade, many studies have focused on discovering these families in Tephritidae species of agricultural importance. However, to date, there is no information on the Mexican fruit fly Anastrepha ludens Loew, a priority pest of quarantine importance in Mexico and other countries. This work represents the first effort to identify, classify and characterize the six chemosensory gene families by analyzing two head transcriptomes of sexually immature and mature adults of A. ludens from laboratory-reared and wild populations, respectively. We identified 120 chemosensory genes encoding 31 Odorant-Binding Proteins (OBPs), 5 Chemosensory Proteins (CSPs), 2 Sensory Neuron Membrane Proteins (SNMPs), 42 Odorant Receptors (ORs), 17 Ionotropic Receptors (IRs), and 23 Gustatory Receptors (GRs). The 120 described chemosensory proteins of the Mexican fruit fly significantly contribute to the genetic databases of insects, particularly dipterans. Except for some OBPs, this work reports for the first time the repertoire of olfactory proteins for one species of the genus Anastrepha, which provides a further basis for studying the olfactory system in the family Tephritidae, one of the most important for its economic and social impact worldwide.

APA, Harvard, Vancouver, ISO, and other styles

5

Kang, Z. W., F. H. Liu, R. P. Pang, W. B. Yu, X. L. Tan, Z. Q. Zheng, H. G. Tian, and T. X. Liu. "The identification and expression analysis of candidate chemosensory genes in the bird cherry-oat aphid Rhopalosiphum padi (L.)." Bulletin of Entomological Research 108, no. 5 (December 4, 2017): 645–57. http://dx.doi.org/10.1017/s0007485317001171.

Full text

Abstract:

AbstractThe bird cherry-oat aphid Rhopalosiphum padi (L.) is one of the most important wheat pests with polyphagia and autumn migrants. And, chemosensory genes were thought to play a key role in insect searching their hosts, food and mate. However, a systematic identification of the chemosensory genes in this pest has not been reported. Thus, in this study, we identified 14 odorant-binding proteins, nine chemosensory proteins, one sensory neuron membrane protein, 15 odorant receptors, 19 gustatory receptors and 16 ionotropic receptors from R. padi transcriptomes with a significantly similarity (E-value < 10−5) to known chemosensory genes in Acyrthosiphon pisum and Aphis gossypii. In addition, real-time quantitative polymerase chain reaction (RT-qPCR) was employed to determine the expression profiles of obtained genes. Among these obtained genes, we selected 23 chemosensory genes to analyze their expression patterns in different tissues, wing morphs and host plants. We found that except RpOBP1, RpOBP3, RpOBP4 and RpOBP5, the rest of the selected genes were highly expressed in the head with antennae compared with body without head and antennae. Besides that, the stimulation and depression of chemosensory genes by plant switch indicated that chemosensory genes might be involved in the plant suitability assessment. These results not only provide insights for the potential roles of chemosensory genes in plant search and perception of R. padi but also provide initial background information for the further research on the molecular mechanism of the polyphagia and autumn migrants of it. Furthermore, these chemosensory genes are also the candidate targets for pest management control in future.

APA, Harvard, Vancouver, ISO, and other styles

6

Liu, Xiaolong, Na Tong, Zheran Wu, Yang Li, Meiqi Ma, Pei Liu, and Min Lu. "Identification of Chemosensory Genes Based on the Antennal Transcriptomic Analysis of Plagiodera versicolora." Insects 13, no. 1 (December 29, 2021): 36. http://dx.doi.org/10.3390/insects13010036.

Full text

Abstract:

Insects can sense surrounding chemical signals by their accurate chemosensory systems. This system plays a vital role in the life history of insects. Several gene families participate in chemosensory processes, including odorant receptors (ORs), ionotropic receptors (IRs), gustatory receptors (GRs), chemosensory proteins (CSPs), odorant binding proteins (OBPs), and sensory neuron membrane proteins (SNMPs). Plagiodera versicolora (Coleoptera: Chrysomelidae), is a leaf-eating forest pest found in salicaceous trees worldwide. In this study, a transcriptome analysis of male and female adult antennae in P. versicolora individuals was conducted, which identified a total of 98 candidate chemosensory genes including 40 ORs, 7 IRs, 13 GRs, 10 CSPs, 24 OBPs, and 4 SNMPs. Subsequently, the tissue expression profiles of 15 P. versicolora OBPs (PverOBPs) and 39 ORs (PverORs) were conducted by quantitative real-time PCR. The data showed that almost all PverOBPs and PverORs were highly expressed in the male and female antennae. In addition, several OBPs and ORs (PverOBP10, PverOBP12, PverOBP18, PverOR24, and PverOR35) had higher expression levels in female antennae than those in the male antennae, indicating that these genes may be taking part in some female-specific behaviors, such as find mates, oviposition site, etc. This study deeply promotes further understanding of the chemosensory system and functional studies of the chemoreception genes in P. versicolora.

APA, Harvard, Vancouver, ISO, and other styles

7

Pelosi, P. "Diversity of Odorant-binding Proteins and Chemosensory Proteins in Insects." Chemical Senses 30, Supplement 1 (January 1, 2005): i291—i292. http://dx.doi.org/10.1093/chemse/bjh229.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Mameli, Marina, Andrea Tuccini, Mario Mazza, Ruggero Petacchi, and Paolo Pelosi. "Soluble proteins in chemosensory organs of phasmids." Insect Biochemistry and Molecular Biology 26, no. 8-9 (September 1996): 875–82. http://dx.doi.org/10.1016/s0965-1748(96)00055-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Picimbon, Jean-François, Karen Dietrich, Heinz Breer, and Jürgen Krieger. "Chemosensory proteins of Locusta migratoria (Orthoptera: Acrididae)." Insect Biochemistry and Molecular Biology 30, no. 3 (March 2000): 233–41. http://dx.doi.org/10.1016/s0965-1748(99)00121-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Agnihotri, Aniruddha, Naiyong Liu, and Wei Xu. "Chemosensory Proteins (CSPs) in the Cotton Bollworm Helicoverpa armigera." Insects 13, no. 1 (December 27, 2021): 29. http://dx.doi.org/10.3390/insects13010029.

Full text

Abstract:

Chemosensory proteins (CSPs) are a family of small, soluble proteins that play a crucial role in transporting odorant and pheromone molecules in the insect chemosensory system. Recent studies reveal that they also function in development, nutrient metabolism and insecticide resistance. In-depth and systematic characterization of previously unknown CSPs will be valuable to investigate more detailed functionalities of this protein family. Here, we identified 27 CSP genes from the genome and transcriptome sequences of cotton bollworm, Helicoverpa armigera (Hübner). The expression patterns of these genes were studied by using transcriptomic data obtained from different tissues and stages. The results demonstrate that H. armigera CSP genes are not only highly expressed in chemosensory tissues, such as antennae, mouthparts, and tarsi, but also in the salivary glands, cuticle epidermis, and hind gut. HarmCSP6 and 22 were selected as candidate CSPs for expression in Escherichia coli and purification. A new method was developed that significantly increased the HarmCSP6 and 22 expression levels as soluble recombinant proteins for purification. This study advances our understanding of insect CSPs and provides a new approach to highly express recombinant CSPs in E. coli.

APA, Harvard, Vancouver, ISO, and other styles

11

Xu, Ji-Wei, Xiu-Yun Zhu, Qiu-Jie Chao, Yong-Jie Zhang, Yu-Xia Yang, Ran-Ran Wang, Yu Zhang, et al. "Chemosensory Gene Families in the Oligophagous Pear Pest Cacopsylla chinensis (Hemiptera: Psyllidae)." Insects 10, no. 6 (June 17, 2019): 175. http://dx.doi.org/10.3390/insects10060175.

Full text

Abstract:

Chemosensory systems play an important role in insect behavior, and some key associated genes have potential as novel targets for pest control. Cacopsylla chinensis is an oligophagous pest and has become one of the main pests of pear trees, but little is known about the molecular-level means by which it locates its hosts. In this study, we assembled the head transcriptome of C. chinensis using Illumina sequencing, and 63,052 Unigenes were identified. A total of 36 candidate chemosensory genes were identified, including five different families: 12 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), 7 odorant receptors (ORs), 4 ionotropic receptors (IRs), and 2 gustatory receptors (GRs). The number of chemosensory gene families is consistent with that found in other Hemipteran species, indicating that our approach successfully obtained the chemosensory genes of C. chinensis. The tissue expression of all genes using quantitative real-time PCR (qRT-PCR) found that some genes displayed male head, female head, or nymph-biased specific/expression. Our results enrich the gene inventory of C. chinensis and provide valuable resources for the analysis of the functions of some key genes. This will help in developing molecular targets for disrupting feeding behavior in C. chinensis.

APA, Harvard, Vancouver, ISO, and other styles

12

Ishida, Yuko, Wataru Tsuchiya, Takeshi Fujii, Zui Fujimoto, Mitsuhiro Miyazawa, Jun Ishibashi, Shigeru Matsuyama, Yukio Ishikawa, and Toshimasa Yamazaki. "Niemann–Pick type C2 protein mediating chemical communication in the worker ant." Proceedings of the National Academy of Sciences 111, no. 10 (February 24, 2014): 3847–52. http://dx.doi.org/10.1073/pnas.1323928111.

Full text

Abstract:

Ants are eusocial insects that are found in most regions of the world. Within its caste, worker ants are responsible for various tasks that are required for colony maintenance. In their chemical communication, α-helical carrier proteins, odorant-binding proteins, and chemosensory proteins, which accumulate in the sensillum lymph in the antennae, play essential roles in transferring hydrophobic semiochemicals to chemosensory receptors. It has been hypothesized that semiochemicals are recognized by α-helical carrier proteins. The number of these proteins, however, is not sufficient to interact with a large number of semiochemicals estimated from chemosensory receptor genes. Here we shed light on this conundrum by identifying a Niemann–Pick type C2 (NPC2) protein from the antenna of the worker Japanese carpenter ant, Camponotus japonicus (CjapNPC2). CjapNPC2 accumulated in the sensillum cavity in the basiconic sensillum. The ligand-binding pocket of CjapNPC2 was composed of a flexible β-structure that allowed it to bind to a wide range of potential semiochemicals. Some of the semiochemicals elicited electrophysiolgical responses in the worker antenna. In vertebrates, NPC2 acts as an essential carrier protein for cholesterol from late endosomes and lysosomes to other cellular organelles. However, the ants have evolved an NPC2 with a malleable ligand-binding pocket as a moderately selective carrier protein in the sensillum cavity of the basiconic sensillum. CjapNPC2 might be able to deliver various hydrophobic semiochemicals to chemosensory receptor neurons and plays crucial roles in chemical communication required to perform the worker ant tasks.

APA, Harvard, Vancouver, ISO, and other styles

13

Calvello, M., A. Brandazza, A. Navarrini, F. R. Dani, S. Turillazzi, A. Felicioli, and P. Pelosi. "Expression of odorant-binding proteins and chemosensory proteins in some Hymenoptera." Insect Biochemistry and Molecular Biology 35, no. 4 (April 2005): 297–307. http://dx.doi.org/10.1016/j.ibmb.2005.01.002.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Mandiana Diakite, Mory, Juan Wang, Suliman Ali, and Man-Qun Wang. "Identification of chemosensory gene families in Rhyzopertha dominica (Coleoptera: Bostrichidae)." Canadian Entomologist 148, no. 1 (May 7, 2015): 8–21. http://dx.doi.org/10.4039/tce.2015.13.

Full text

Abstract:

AbstractChemoreception is a key process for insects. Odorant messages diffuse through the air and are translated into physiological signals by chemosensory receptor neurons in sensilla that are mainly located on insect antennae. We sequenced the antenna transcriptome of Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), which is a serious pest of stored grains throughout regions with warm climates, and performed transcriptome analysis on R. dominica antennae. We obtained 57 million 90-base pair-long reads that we assembled into 37 877 unigenes with a mean size of 1007 base pairs. Predicted protein sequences were matched with Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) (79.1%), Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae) (1.7%), Megachile rotundata (Fabricius) (Hymenoptera: Megachilidae) (1.3%), Acyrthosiphon pisum Harris (Hemiptera: Aphididae) (1.2%), and other (16.7%) homologues. In chemosensory gene families, we identified transcripts that encoded the following putative genes: 12 odorant-binding proteins (OBPs), four pheromone-binding proteins (PBPs), eight chemosensory proteins (CSPs), five sensory neuron membrane proteins (SNMPs), six odorant receptors, and eight ionotropic receptors. The diversity of the predicted OBPs, PBPs, and CSPs are also discussed. These findings will advance our understanding of olfaction process by this pest.

APA, Harvard, Vancouver, ISO, and other styles

15

Rihani, Karen, Jean-François Ferveur, and Loïc Briand. "The 40-Year Mystery of Insect Odorant-Binding Proteins." Biomolecules 11, no. 4 (March 30, 2021): 509. http://dx.doi.org/10.3390/biom11040509.

Full text

Abstract:

The survival of insects depends on their ability to detect molecules present in their environment. Odorant-binding proteins (OBPs) form a family of proteins involved in chemoreception. While OBPs were initially found in olfactory appendages, recently these proteins were discovered in other chemosensory and non-chemosensory organs. OBPs can bind, solubilize and transport hydrophobic stimuli to chemoreceptors across the aqueous sensilla lymph. In addition to this broadly accepted “transporter role”, OBPs can also buffer sudden changes in odorant levels and are involved in hygro-reception. The physiological roles of OBPs expressed in other body tissues, such as mouthparts, pheromone glands, reproductive organs, digestive tract and venom glands, remain to be investigated. This review provides an updated panorama on the varied structural aspects, binding properties, tissue expression and functional roles of insect OBPs.

APA, Harvard, Vancouver, ISO, and other styles

16

Liu, Guoxia, Philippe Arnaud, Bernard Offmann, and Jean-François Picimbon. "Genotyping and Bio-Sensing Chemosensory Proteins in Insects." Sensors 17, no. 8 (August 4, 2017): 1801. http://dx.doi.org/10.3390/s17081801.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Nagnan-Le Meillour, P. "Chemosensory Proteins from the Proboscis of Mamestra brassicae." Chemical Senses 25, no. 5 (October 1, 2000): 541–53. http://dx.doi.org/10.1093/chemse/25.5.541.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Iovinella, Immacolata, Francesco Bozza, Beniamino Caputo, Alessandra della Torre, and Paolo Pelosi. "Ligand-Binding Study of Anopheles gambiae Chemosensory Proteins." Chemical Senses 38, no. 5 (April 17, 2013): 409–19. http://dx.doi.org/10.1093/chemse/bjt012.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Mombaerts, P. "Seven-Transmembrane Proteins as Odorant and Chemosensory Receptors." Science 286, no. 5440 (October 22, 1999): 707–11. http://dx.doi.org/10.1126/science.286.5440.707.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Peng, Yong, Shan-Ning Wang, Ke-Ming Li, Jing-Tao Liu, Yao Zheng, Shuang Shan, Ye-Qing Yang, Rui-Jun Li, Yong-Jun Zhang, and Yu-Yuan Guo. "Identification of odorant binding proteins and chemosensory proteins in Microplitis mediator as well as functional characterization of chemosensory protein 3." PLOS ONE 12, no. 7 (July 21, 2017): e0180775. http://dx.doi.org/10.1371/journal.pone.0180775.

Full text

APA, Harvard, Vancouver, ISO, and other styles

21

Roncalli, Vittoria, Marco Uttieri, Iole Di Capua, Chiara Lauritano, and Ylenia Carotenuto. "Chemosensory-Related Genes in Marine Copepods." Marine Drugs 20, no. 11 (October 29, 2022): 681. http://dx.doi.org/10.3390/md20110681.

Full text

Abstract:

Living organisms deeply rely on the acquisition of chemical signals in any aspect of their life, from searching for food, mating and defending themselves from stressors. Copepods, the most abundant and ubiquitous metazoans on Earth, possess diversified and highly specified chemoreceptive structures along their body. The detection of chemical stimuli activates specific pathways, although this process has so far been analyzed only on a relatively limited number of species. Here, in silico mining of 18 publicly available transcriptomes is performed to delve into the copepod chemosensory genes, improving current knowledge on the diversity of this multigene family and on possible physiological mechanisms involved in the detection and analysis of chemical cues. Our study identifies the presence of ionotropic receptors, chemosensory proteins and gustatory receptors in copepods belonging to the Calanoida, Cyclopoida and Harpacticoida orders. We also confirm the absence in these copepods of odorant receptors and odorant-binding proteins agreeing with their insect specificity. Copepods have evolved several mechanisms to survive in the harsh marine environment such as producing proteins to respond to external stimulii. Overall, the results of our study open new possibilities for the use of the chemosensory genes as biomarkers in chemical ecology studies on copepods and possibly also in other marine holozooplankters.

APA, Harvard, Vancouver, ISO, and other styles

22

Matilla, Miguel A., Roberta Genova, David Martín-Mora, Sandra Maaβ, Dörte Becher, and Tino Krell. "The Cellular Abundance of Chemoreceptors, Chemosensory Signaling Proteins, Sensor Histidine Kinases, and Solute Binding Proteins of Pseudomonas aeruginosa Provides Insight into Sensory Preferences and Signaling Mechanisms." International Journal of Molecular Sciences 24, no. 2 (January 10, 2023): 1363. http://dx.doi.org/10.3390/ijms24021363.

Full text

Abstract:

Chemosensory pathways and two-component systems are important bacterial signal transduction systems. In the human pathogen Pseudomonas aeruginosa, these systems control many virulence traits. Previous studies showed that inorganic phosphate (Pi) deficiency induces virulence. We report here the abundance of chemosensory and two-component signaling proteins of P. aeruginosa grown in Pi deficient and sufficient media. The cellular abundance of chemoreceptors differed greatly, since a 2400-fold difference between the most and least abundant receptors was observed. For many chemoreceptors, their amount varied with the growth condition. The amount of chemoreceptors did not correlate with the magnitude of chemotaxis to their cognate chemoeffectors. Of the four chemosensory pathways, proteins of the Che chemotaxis pathway were most abundant and showed little variation in different growth conditions. The abundance of chemoreceptors and solute binding proteins indicates a sensing preference for amino acids and polyamines. There was an excess of response regulators over sensor histidine kinases in two-component systems. In contrast, ratios of the response regulators CheY and CheB to the histidine kinase CheA of the Che pathway were all below 1, indicative of different signaling mechanisms. This study will serve as a reference for exploring sensing preferences and signaling mechanisms of other bacteria.

APA, Harvard, Vancouver, ISO, and other styles

23

Höfer, Dirk, Esther Asan, and Detlev Drenckhahn. "Chemosensory Perception in the Gut." Physiology 14, no. 1 (February 1999): 18–23. http://dx.doi.org/10.1152/physiologyonline.1999.14.1.18.

Full text

Abstract:

The ability of the gut mucosa to sense the chemical composition of chyme is important for gastrointestinal functions. The demonstration of gustducin and transducin, two α-subunits of GTP-binding proteins involved in gustatory signal transduction, in gastrointestinal epithelial cells provides first clues to the molecular basis of enteric chemosensitivity. Nitric oxide may play a role as a secondary messenger.

APA, Harvard, Vancouver, ISO, and other styles

24

Li, Lu-Lu, Ji-Wei Xu, Wei-Chen Yao, Hui-Hui Yang, Youssef Dewer, Fan Zhang, Xiu-Yun Zhu, and Ya-Nan Zhang. "Chemosensory genes in the head of Spodoptera litura larvae." Bulletin of Entomological Research 111, no. 4 (February 26, 2021): 454–63. http://dx.doi.org/10.1017/s0007485321000109.

Full text

Abstract:

AbstractThe tobacco cutworm Spodoptera litura (Lepidoptera: Noctuidae) is a polyphagous pest with a highly selective and sensitive chemosensory system involved in complex physiological behaviors such as searching for food sources, feeding, courtship, and oviposition. However, effective management strategies for controlling the insect pest populations under threshold levels are lacking. Therefore, there is an urgent need to formulate eco-friendly pest control strategies based on the disruption of the insect chemosensory system. In this study, we identified 158 putative chemosensory genes based on transcriptomic and genomic data for S. litura, including 45 odorant-binding proteins (OBPs, nine were new), 23 chemosensory proteins (CSPs), 60 odorant receptors (ORs, three were new), and 30 gustatory receptors (GRs, three were new), a number higher than those reported by previous transcriptome studies. Subsequently, we constructed phylogenetic trees based on these genes in moths and analyzed the dynamic expression of various genes in head capsules across larval instars using quantitative real-time polymerase chain reaction. Nine genes–SlitOBP8, SlitOBP9, SlitOBP25, SlitCSP1, SlitCSP7, SlitCSP18, SlitOR34, SlitGR240, and SlitGR242–were highly expressed in the heads of 3- to 5-day-old S. litura larvae. The genes differentially expressed in olfactory organs during larval development might play crucial roles in the chemosensory system of S. litura larvae. Our findings substantially expand the gene inventory for S. litura and present potential target genes for further studies on larval feeding in S. litura.

APA, Harvard, Vancouver, ISO, and other styles

25

Vizueta, Joel, Paula Escuer, Cristina Frías-López, Sara Guirao-Rico, Lars Hering, Georg Mayer, Julio Rozas, and Alejandro Sánchez-Gracia. "Evolutionary History of Major Chemosensory Gene Families across Panarthropoda." Molecular Biology and Evolution 37, no. 12 (August 4, 2020): 3601–15. http://dx.doi.org/10.1093/molbev/msaa197.

Full text

Abstract:

Abstract Chemosensory perception is a fundamental biological process of particular relevance in basic and applied arthropod research. However, apart from insects, there is little knowledge of specific molecules involved in this system, which is restricted to a few taxa with uneven phylogenetic sampling across lineages. From an evolutionary perspective, onychophorans (velvet worms) and tardigrades (water bears) are of special interest since they represent the closest living relatives of arthropods, altogether comprising the Panarthropoda. To get insights into the evolutionary origin and diversification of the chemosensory gene repertoire in panarthropods, we sequenced the antenna- and head-specific transcriptomes of the velvet worm Euperipatoides rowelli and analyzed members of all major chemosensory families in representative genomes of onychophorans, tardigrades, and arthropods. Our results suggest that the NPC2 gene family was the only family encoding soluble proteins in the panarthropod ancestor and that onychophorans might have lost many arthropod-like chemoreceptors, including the highly conserved IR25a receptor of protostomes. On the other hand, the eutardigrade genomes lack genes encoding the DEG-ENaC and CD36-sensory neuron membrane proteins, the chemosensory members of which have been retained in arthropods; these losses might be related to lineage-specific adaptive strategies of tardigrades to survive extreme environmental conditions. Although the results of this study need to be further substantiated by an increased taxon sampling, our findings shed light on the diversification of chemosensory gene families in Panarthropoda and contribute to a better understanding of the evolution of animal chemical senses.

APA, Harvard, Vancouver, ISO, and other styles

26

Wu, Zheran, Na Tong, Yang Li, Jinmeng Guo, Min Lu, and Xiaolong Liu. "Foreleg Transcriptomic Analysis of the Chemosensory Gene Families in Plagiodera versicolora (Coleoptera: Chrysomelidae)." Insects 13, no. 9 (August 24, 2022): 763. http://dx.doi.org/10.3390/insects13090763.

Full text

Abstract:

Plagiodera versicolora (Coleoptera: Chrysomelidae) is a worldwide leaf-eating forest pest in salicaceous trees. The forelegs play important roles in the chemoreception of insects. In this study, we conducted a transcriptome analysis of adult forelegs in P. versicolora and identified a total of 53 candidate chemosensory genes encoding 4 chemosensory proteins (CSPs), 19 odorant binding proteins (OBPs), 10 odorant receptors (ORs), 10 gustatory receptors (GRs), 6 ionotropic receptors (IRs), and 4 sensory neuron membrane proteins (SNMPs). Compared with the previous antennae transcriptome data, 1 CSP, 4 OBPs, 1 OR, 3 IRs, and 4 GRs were newly identified in the forelegs. Subsequently, the tissue expression profiles of 10 P. versicolora chemosensory genes were performed by real-time quantitative PCR. The results showed that PverOBP25, PverOBP27, and PverCSP6 were highly expressed in the antennae of both sexes. PverCSP11 and PverIR9 are predominately expressed in the forelegs than in the antennae. In addition, the expression levels of PverGR15 in female antennae and forelegs were significantly higher than those in the male antennae, implying that it may be involved in some female-specific behaviors such as oviposition site seeking. This work would greatly further the understanding of the chemoreception mechanism in P. versicolora.

APA, Harvard, Vancouver, ISO, and other styles

27

Li, Xiaowei, Jianghui Cheng, Limin Chen, Jun Huang, Zhijun Zhang, Jinming Zhang, Xiaoyun Ren, et al. "Comparison and Functional Analysis of Odorant-Binding Proteins and Chemosensory Proteins in Two Closely Related Thrips Species, Frankliniella occidentalis and Frankliniella intonsa (Thysanoptera: Thripidae) Based on Antennal Transcriptome Analysis." International Journal of Molecular Sciences 23, no. 22 (November 11, 2022): 13900. http://dx.doi.org/10.3390/ijms232213900.

Full text

Abstract:

Two closely related thrips species, Frankliniella occidentalis and Frankliniella intonsa, are important pests on agricultural and horticultural crops. They have several similarities, including occurrence patterns, host range, and aggregation pheromone compounds. However, there are very few reports about the chemosensory genes and olfactory mechanisms in these two species. To expand our knowledge of the thrips chemosensory system, we conducted antennal transcriptome analysis of two thrips species, and identified seven odorant-binding proteins (OBPs) and eight chemosensory proteins (CSPs) in F. occidentalis, as well as six OBPs and six CSPs in F. intonsa. OBPs and CSPs showed high sequence identity between the two thrips species. The RT-qPCR results showed that the orthologous genes FoccOBP1/3/4/5/6, FintOBP1/3/4/6, FoccCSP1/2/3, and FintCSP1/2 were highly expressed in male adults. Molecular docking results suggested that orthologous pairs FoccOBP4/FintOBP4, FoccOBP6/FintOBP6, and FoccCSP2/FintCSP2 might be involved in transporting the major aggregation pheromone compound neryl (S)-2-methylbutanoate, while orthologous pairs FoccOBP6/FintOBP6, FoccCSP2/FintCSP2, and FoccCSP3/FintCSP3 might be involved in transporting the minor aggregation pheromone compound (R)-lavandulyl acetate. These results will provide a fundamental basis for understanding the molecular mechanisms of pheromone reception in the two thrips species.

APA, Harvard, Vancouver, ISO, and other styles

28

Bruch, R. C., and D. L. Kalinoski. "Interaction of GTP-binding regulatory proteins with chemosensory receptors." Journal of Biological Chemistry 262, no. 5 (February 1987): 2401–4. http://dx.doi.org/10.1016/s0021-9258(18)61669-2.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Matsunami, Hiroaki, Joel D. Mainland, and Sandeepa Dey. "Trafficking of Mammalian Chemosensory Receptors by Receptor-transporting Proteins." Annals of the New York Academy of Sciences 1170, no. 1 (July 2009): 153–56. http://dx.doi.org/10.1111/j.1749-6632.2009.03888.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Shitashiro, Maiko, Hirohide Tanaka, Chang soo Hong, Akio Kuroda, Noboru Takiguchi, Hisao Ohtake, and Junichi Kato. "Identification of chemosensory proteins for trichloroethylene in Pseudomonas aeruginosa." Journal of Bioscience and Bioengineering 99, no. 4 (April 2005): 396–402. http://dx.doi.org/10.1263/jbb.99.396.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Strotmann, J. "Olfactory Receptor Proteins in Axonal Processes of Chemosensory Neurons." Journal of Neuroscience 24, no. 35 (September 1, 2004): 7754–61. http://dx.doi.org/10.1523/jneurosci.2588-04.2004.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Celorio-Mancera, Maria de la Paz, Sara M. Sundmalm, Heiko Vogel, Dorothea Rutishauser, A. Jimmy Ytterberg, Roman A. Zubarev, and Niklas Janz. "Chemosensory proteins, major salivary factors in caterpillar mandibular glands." Insect Biochemistry and Molecular Biology 42, no. 10 (October 2012): 796–805. http://dx.doi.org/10.1016/j.ibmb.2012.07.008.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Picimbon, Jean-Fran�ois, Karen Dietrich, Sergio Angeli, Andrea Scaloni, J�rgen Krieger, Heinz Breer, and Paolo Pelosi. "Purification and molecular cloning of chemosensory proteins fromBombyx mori." Archives of Insect Biochemistry and Physiology 44, no. 3 (2000): 120–29. http://dx.doi.org/10.1002/1520-6327(200007)44:3<120::aid-arch3>3.0.co;2-h.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Yuan, Xin, Yan-Dong Jiang, Gui-Yao Wang, Hang Yu, Wen-Wu Zhou, Su Liu, Mao-Fa Yang, et al. "Odorant-Binding Proteins and Chemosensory Proteins from an Invasive PestLissorhoptrus oryzophilus(Coleoptera: Curculionidae)." Environmental Entomology 45, no. 5 (August 28, 2016): 1276–86. http://dx.doi.org/10.1093/ee/nvw111.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Ballesteros, Gabriel I., Daniela A. Sepúlveda, and Christian C. Figueroa. "Identification and Expression Profiling of Peripheral Olfactory Genes in the Parasitoid Wasp Aphidius ervi (Hymenoptera: Braconidae) Reared on Different Aphid Hosts." Insects 10, no. 11 (November 8, 2019): 397. http://dx.doi.org/10.3390/insects10110397.

Full text

Abstract:

Generalist parasitoids of aphids, such as the wasp Aphidius ervi, display significant differences in terms of host preference and host acceptance, depending on the host on which they developed (natal host), which is preferred over a non-natal host, a trait known as host fidelity. This trait allows females to quickly find hosts in heterogeneous environments, a process mediated by chemosensory/olfactory mechanisms, as parasitoids rely on olfaction and chemical cues during host selection. Thus, it is expected that proteins participating in chemosensory recognition, such as odorant-binding proteins (OBPs) and odorant receptors (ORs) would play a key role in host preference. In this study, we addressed the effect of parasitoid reciprocal host switching between two aphid hosts (Sitobion avenae and Acyrthosiphon pisum) on the expression patterns of chemosensory genes in the wasp A. ervi. First, by using a transcriptomic approach based on RNAseq of A. ervi females reared on S. avenae and A. pisum, we were able to annotate a total of 91 transcripts related to chemoperception. We also performed an in-silico expression analysis and found three OBPs and five ORs displaying different expression levels. Then, by using qRT-PCR amplification, we found significant differences in the expression levels of these eight genes when the parasitoids were reciprocally transplanted from S. avenae onto A. pisum and vice versa. This suggests that the expression levels of genes coding for odorant receptors and odorant-binding proteins would be regulated by the specific plant–aphid host complex where the parasitoids develop (maternal previous experience) and that chemosensory genes coding for olfactory mechanisms would play a crucial role on host preference and host acceptance, ultimately leading to the establishment of host fidelity in A. ervi parasitoids.

APA, Harvard, Vancouver, ISO, and other styles

36

Chang, Hetan, Dong Ai, Jin Zhang, Shuanglin Dong, Yang Liu, and Guirong Wang. "Candidate odorant binding proteins and chemosensory proteins in the larval chemosensory tissues of two closely related noctuidae moths, Helicoverpa armigera and H. assulta." PLOS ONE 12, no. 6 (June 8, 2017): e0179243. http://dx.doi.org/10.1371/journal.pone.0179243.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Zhou, Xiang, Jixing Guo, Mingxia Zhang, Chunxiu Bai, Zheng Wang, and Zhidong Li. "Antennal transcriptome analysis and candidate olfactory genes in Crematogaster rogenhoferi." Bulletin of Entomological Research 111, no. 4 (March 11, 2021): 464–75. http://dx.doi.org/10.1017/s0007485321000134.

Full text

Abstract:

AbstractCrematogaster rogenhoferi (Hymenoptera: Formicidae), an omnivorous ant, is one of the dominant predatory natural enemies of a soft scale pest, Parasaissetia nigra Nietner (Homoptera: Coccidae), and can effectively control P. nigra populations in rubber forests. Olfaction plays a vital role in the process of predation. However, the information about the molecular mechanism of olfaction-evoked behaviour in C. rogenhoferi is limited. In this study, we conducted antennal transcriptome analysis to identify candidate olfactory genes. We obtained 53,892 unigenes, 16,185 of which were annotated. Based on annotations, we identified 49 unigenes related to chemoreception, including four odourant-binding proteins, three chemosensory proteins, 37 odourant receptors, two odourant ionotropic receptors and three sensory neuron membrane proteins. This is the first report on the molecular basis of the chemosensory system of C. rogenhoferi. The findings provide a basis for elucidating the molecular mechanisms of the olfactory-related behaviours of C. rogenhoferi, which would facilitate a better application of C. rogenhoferi as a biological control agent.

APA, Harvard, Vancouver, ISO, and other styles

38

Wu, Lixian, Xin Zhai, Liangbin Li, Qiang Li, Fang Liu, and Hongxia Zhao. "Identification and Expression Profile of Chemosensory Genes in the Small Hive Beetle Aethina tumida." Insects 12, no. 8 (July 21, 2021): 661. http://dx.doi.org/10.3390/insects12080661.

Full text

Abstract:

Aethina tumida is a parasite and predator of honeybee causing severe loss to the bee industry. No effective and environmentally friendly methods are available to control this pest at present. Chemosensory genes play key roles in insect behavior which can potentially be used as targets for developing environmentally friendly pest control agents. In this study, the putative chemosensory genes in antennae and forelegs of A. tumida involved in olfaction or contact chemical communication of adults were investigated using RNA transcriptome sequencing and PCR methods. Based on transcriptomic data, unigenes encoding 38 odorant receptors (ORs), 24 ionotropic receptors (IRs), 14 gustatory receptors (GRs), 3 sensory neuron membrane proteins (SNMPs), 29 odorant binding proteins (OBPs), and 22 chemosensory proteins (CSPs) were identified. The analyses of tissue expression profiles revealed that genes encoding 38 ORs, 13 antennal IRs, 11 GRs, 1 SNMP, 24 OBPs, and 12 CSPs were predominately expressed in antennae. No significant differences in expression levels of these genes were found between males and females. Genes encoding 5 non-NMDA iGluRs, 3 GRs, 2 SNMPs, 5 OBPs, and 12 CSPs were predominately expressed in forelegs. RT-PCR assays for SNMPs, OBPs and CSPs further revealed that 3 OBPs (AtumOBP3, 26 and 28) and 3 CSPs (AtumCSP7, 8 and 21) were highly expressed in antennae. Our results enrich the gene inventory of A. tumida and facilitate the discovery of potential novel targets for developing new pest control measures.

APA, Harvard, Vancouver, ISO, and other styles

39

Büch, Thomas, Eva Büch, Ingrid Boekhoff, Dirk Steinritz, and Achim Aigner. "Role of Chemosensory TRP Channels in Lung Cancer." Pharmaceuticals 11, no. 4 (September 21, 2018): 90. http://dx.doi.org/10.3390/ph11040090.

Full text

Abstract:

Transient receptor potential (TRP) channels represent a large family of cation channels and many members of the TRP family have been shown to act as polymodal receptor molecules for irritative or potentially harmful substances. These chemosensory TRP channels have been extensively characterized in primary sensory and neuronal cells. However, in recent years the functional expression of these proteins in non-neuronal cells, e.g., in the epithelial lining of the respiratory tract has been confirmed. Notably, these proteins have also been described in a number of cancer types. As sensor molecules for noxious compounds, chemosensory TRP channels are involved in cell defense mechanisms and influence cell survival following exposure to toxic substances via the modulation of apoptotic signaling. Of note, a number of cytostatic drugs or drug metabolites can activate these TRP channels, which could affect the therapeutic efficacy of these cytostatics. Moreover, toxic inhalational substances with potential involvement in lung carcinogenesis are well established TRP activators. In this review, we present a synopsis of data on the expression of chemosensory TRP channels in lung cancer cells and describe TRP agonists and TRP-dependent signaling pathways with potential relevance to tumor biology. Furthermore, we discuss a possible role of TRP channels in the non-genomic, tumor-promoting effects of inhalational carcinogens such as cigarette smoke.

APA, Harvard, Vancouver, ISO, and other styles

40

Wang, Q., J. J. Zhou, J. T. Liu, G. Z. Huang, W. Y. Xu, Q. Zhang, J. L. Chen, Y. J. Zhang, X. C. Li, and S. H. Gu. "Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids." Insect Molecular Biology 28, no. 1 (October 5, 2018): 1–22. http://dx.doi.org/10.1111/imb.12513.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Yu, Yanxue, Shangan Zhang, Long Zhang, and Xingbo Zhao. "Developmental expression of odorant-binding proteins and chemosensory proteins in the embryos ofLocusta migratoria." Archives of Insect Biochemistry and Physiology 71, no. 2 (June 2009): 105–15. http://dx.doi.org/10.1002/arch.20303.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Zhu, Jiao, Immacolata Iovinella, Francesca Romana Dani, Yu-Ling Liu, Ling-Qiao Huang, Yang Liu, Chen-Zhu Wang, Paolo Pelosi, and Guirong Wang. "Conserved chemosensory proteins in the proboscis and eyes of Lepidoptera." International Journal of Biological Sciences 12, no. 11 (2016): 1394–404. http://dx.doi.org/10.7150/ijbs.16517.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Marchese, Silvana, Sergio Angeli, Annapaola Andolfo, Andrea Scaloni, Anna Brandazza, Mario Mazza, Jean-François Picimbon, Walter S. Leal, and Paolo Pelosi. "Soluble proteins from chemosensory organs of Eurycantha calcarata (Insects, Phasmatodea)." Insect Biochemistry and Molecular Biology 30, no. 11 (November 2000): 1091–98. http://dx.doi.org/10.1016/s0965-1748(00)00084-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Abedrabbo, Samar, Juan Castellon, Kieran D. Collins, Kevin S. Johnson, and Karen M. Ottemann. "Cooperation of two distinct coupling proteins creates chemosensory network connections." Proceedings of the National Academy of Sciences 114, no. 11 (February 27, 2017): 2970–75. http://dx.doi.org/10.1073/pnas.1618227114.

Full text

Abstract:

Although it is appreciated that bacterial chemotaxis systems rely on coupling, also called scaffold, proteins to both connect input receptors with output kinases and build interkinase connections that allow signal amplification, it is not yet clear why many systems use more than one coupling protein. We examined the distinct functions for multiple coupling proteins in the bacterial chemotaxis system of Helicobacter pylori, which requires two nonredundant coupling proteins for chemotaxis: CheW and CheV1, a hybrid of a CheW and a phosphorylatable receiver domain. We report that CheV1 and CheW have largely redundant abilities to interact with chemoreceptors and the CheA kinase, and both similarly activated CheA’s kinase activity. We discovered, however, that they are not redundant for formation of the higher order chemoreceptor arrays that are known to form via CheA–CheW interactions. In support of this possibility, we found that CheW and CheV1 interact with each other and with CheA independent of the chemoreceptors. Therefore, it seems that some microbes have modified array formation to require CheW and CheV1. Our data suggest that multiple coupling proteins may be used to provide flexibility in the chemoreceptor array formation.

APA, Harvard, Vancouver, ISO, and other styles

45

Poivet, Erwan, Aurore Gallot, Nicolas Montagné, Pavel Senin, Christelle Monsempès, Fabrice Legeai, and Emmanuelle Jacquin-Joly. "Transcriptome Profiling of Starvation in the Peripheral Chemosensory Organs of the Crop Pest Spodoptera littoralis Caterpillars." Insects 12, no. 7 (June 23, 2021): 573. http://dx.doi.org/10.3390/insects12070573.

Full text

Abstract:

Starvation is frequently encountered by animals under fluctuating food conditions in nature, and response to it is vital for life span. Many studies have investigated the behavioral and physiological responses to starvation. In particular, starvation is known to induce changes in olfactory behaviors and olfactory sensitivity to food odorants, but the underlying mechanisms are not well understood. Here, we investigated the transcriptional changes induced by starvation in the chemosensory tissues of the caterpillar Spodoptera littoralis, using Illumina RNA sequencing. Gene expression profiling revealed 81 regulated transcripts associated with several biological processes, such as glucose metabolism, immune defense, response to stress, foraging activity, and olfaction. Focusing on the olfactory process, we observed changes in transcripts encoding proteins putatively involved in the peri-receptor events, namely, chemosensory proteins and odorant-degrading enzymes. Such modulation of their expression may drive fluctuations in the dynamics and the sensitivity of the olfactory receptor neuron response. In combination with the enhanced presynaptic activity mediated via the short neuropeptide F expressed during fasting periods, this could explain an enhanced olfactory detection process. Our observations suggest that a coordinated transcriptional response of peripheral chemosensory organs participates in the regulation of olfactory signal reception and olfactory-driven behaviors upon starvation.

APA, Harvard, Vancouver, ISO, and other styles

46

Olafson, Pia Untalan, and Christopher A. Saski. "Chemosensory-Related Gene Family Members of the Horn Fly, Haematobia irritans irritans (Diptera: Muscidae), Identified by Transcriptome Analysis." Insects 11, no. 11 (November 19, 2020): 816. http://dx.doi.org/10.3390/insects11110816.

Full text

Abstract:

Horn flies are one of the most significant economic pests of cattle in the United States and worldwide. Chemical control methods have been routinely utilized to reduce populations of this pest, but the steady development of insecticide resistance has prompted evaluation of alternative control strategies. Behavior modifying compounds from natural products have shown some success in impacting horn fly populations, and a more thorough understanding of the horn fly chemosensory system would enable improvements in the development of species-specific compounds. Using an RNA-seq approach, we assembled a transcriptome representing genes expressed in adult female and male horn fly head appendages (antennae, maxillary palps, and proboscides) and adult fly bodies from which heads were removed. Differential gene expression analysis identified chemosensory gene family members that were enriched in head appendage tissues compared with headless bodies. Candidate members included 43 odorant binding proteins (OBP) and 5 chemosensory binding proteins (CSP), as well as 44 odorant receptors (OR), 27 gustatory receptors (GR), and 34 ionotropic receptors (IR). Sex-biased expression of these genes was not observed. These findings provide a resource to enable future studies targeting horn fly chemosensation as part of an integrated strategy to control this blood-feeding pest.

APA, Harvard, Vancouver, ISO, and other styles

47

Zhang, Shen, Zhang, Wang, Kong, Liu, and Zhang. "Chemosensory Characteristics of Two Semanotus bifasciatus Populations." Forests 10, no. 8 (August 2, 2019): 655. http://dx.doi.org/10.3390/f10080655.

Full text

Abstract:

Semanotus bifasciatus (Motschulsky) (Cerambycidae: Coleoptera) is a major forest borer in China, and attractants provide a promising method for the control of this pest. Exploration of the chemosensory mechanisms of S. bifasciatus is important for the development of efficient attractants for this pest. However, little information is available about the olfactory mechanisms of S. bifasciatus. Previous research has indicated that the trapping effects of the same attractant are different between Beijing and Shandong populations of S. bifasciatus. To explore the reasons for this, next-generation sequencing was performed to analyze the antennal transcriptome of both sexes of the two S. bifasciatus populations, and the olfactory-related genes were identified. Furthermore, the expression levels and single nucleotide polymorphisms (SNPs) of the olfactory-related genes between the two populations were compared. We found that the expression levels of odorant binding proteins (OBPs), odorant receptors (ORs), and sensory neuron membrane proteins (SNMPs) in male S. bifasciatus of the Beijing population were obviously lower than those in the Shandong population, and most of the conserved SNPs in OBPs and ORs of the two populations showed more diversity in the Beijing population. Our work provides a foundation for future research of the molecular olfactory mechanisms and pest management of S. bifasciatus, as well as other longhorn beetles.

APA, Harvard, Vancouver, ISO, and other styles

48

Lucero, Mary T., Wei Huang, and Tu Dang. "Immunohistochemical evidence for the Na + /Ca 2+ exchanger in squid olfactory neurons." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 355, no. 1401 (September 29, 2000): 1215–18. http://dx.doi.org/10.1098/rstb.2000.0670.

Full text

Abstract:

The olfactory organs from the squid Lolliguncula brevis are composed of a pseudostratified epithelium containing five morphological subtypes of chemosensory neurons and ciliated support cells. Physiological recordings have been made from two of the subtypes and only the type 4 neuron has been studied in detail. Odour–stimulated increases in intracellular calcium and rapid activation of an electrogenic Na + /Ca 2+ exchanger current in type 4 neurons suggest that the exchanger proteins are localized very close to the transduction machinery. Electrophysiological studies have shown that olfactory signal transduction takes place in the apical ciliary regions of olfactory neurons. Using polyclonal antiserum against squid Na + /Ca 2+ proteins, we observed specific staining in the ciliary region of cells that resemble type 2, 3, 4 and 5 neurons. Staining was also observed in axon bundles, and in muscle tissue. Collectively, these data support the model that Na + /Ca 2+ exchanger proteins are localized to transduction machinery in cilia of type 4 neurons and suggest that the other olfactory subtypes also use Ca 2+ during chemosensory responses.

APA, Harvard, Vancouver, ISO, and other styles

49

Kim, Min-Su, Allen Repp, and Dean P. Smith. "LUSH Odorant-Binding Protein Mediates Chemosensory Responses to Alcohols in Drosophila melanogaster." Genetics 150, no. 2 (October 1, 1998): 711–21. http://dx.doi.org/10.1093/genetics/150.2.711.

Full text

Abstract:

Abstract The molecular mechanisms mediating chemosensory discrimination in insects are unknown. Using the enhancer trapping approach, we identified a new Drosophila mutant, lush, with odorant-specific defects in olfactory behavior. lush mutant flies are abnormally attracted to high concentrations of ethanol, propanol, and butanol but have normal chemosensory responses to other odorants. We show that wild-type flies have an active olfactory avoidance mechanism to prevent attraction to concentrated alcohol, and this response is defective in lush mutants. This suggests that the defective olfactory behavior associated with the lush mutation may result from a specific defect in chemoavoidance. lush mutants have a 3-kb deletion that produces a null allele of a new member of the invertebrate odorant-binding protein family, LUSH. LUSH is normally expressed exclusively in a subset of trichoid chemosensory sensilla located on the ventral-lateral surface of the third antennal segment. LUSH is secreted from nonneuronal support cells into the sensillum lymph that bathes the olfactory neurons within these sensilla. Reintroduction of a cloned wild-type copy of lush into the mutant background completely restores wild-type olfactory behavior, demonstrating that this odorant-binding protein is required in a subset of sensilla for normal chemosensory behavior to a subset of odorants. These findings provide direct evidence that odorant-binding proteins are required for normal chemosensory behavior in Drosophila and may partially determine the chemical specificity of olfactory neurons in vivo.

APA, Harvard, Vancouver, ISO, and other styles

50

King, Kendall, Megan E. Meuti, and Norman F. Johnson. "Identification and expression of odorant binding proteins in the egg-parasitoid Trissolcus basalis (Wollaston) (Hymenoptera, Scelionidae, Telenominae)." Journal of Hymenoptera Research 87 (December 23, 2021): 251–66. http://dx.doi.org/10.3897/jhr.87.68954.

Full text

Abstract:

Trissolcus basalis (Wollaston) (Hymenoptera, Scelionidae) is an egg-parasitoid of the southern green stink bug, Nezara viridula (Linneaus) (Hemiptera, Pentatomidae). Many behaviors associated with female T. basalis host-finding and acceptance are mediated by chemosensory pathways, for which olfactory, gustatory and ionotropic receptors have been previously identified. Odorant binding proteins (OBPs) are small, globular proteins, one of the functions of which is the transport of odorant ligands through the aqueous lymph of chemosensory sensilla to these receptors. We identified 18 classical OBP sequences in the T. basalis genome and transcriptomes sharing an average 26.8% pairwise identity. Gene tree analyses suggest very limited lineage-specific expansion and identify potential orthologs among other scelionids and Hymenoptera. Transcriptome mapping and qPCR comparison of expression levels in antennae and bodies of both sexes determine that at least five TbOBPs are preferentially expressed in the female antennae. These are, therefore, prime candidates for further study to determine their role in detecting host-produced semiochemicals.

APA, Harvard, Vancouver, ISO, and other styles

Journal articles on the topic 'Chemosensory proteins'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles