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Journal articles on the topic 'Chemiluminescent'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Ibragimova, D. A., O. M. Kamil, T. V. Yankova, N. A. Yashtulov, and N. K. Zaitsev. "THE EFFECT OF SURFACTANTS ON THE CHEMILUMINESCENT REACTION OF LUMINOL WITH HYDROGEN PEROXIDE." Fine Chemical Technologies 12, no. 6 (December 28, 2017): 71–76. http://dx.doi.org/10.32362/2410-6593-2017-12-6-71-76.

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The luminol-hydrogen peroxide chemiluminescent system is widely used for the creation of diagnostic systems, for chemical analysis, for studying the kinetics and mechanisms of chemical reactions, for the creation of special and emergency light sources, and for monitoring living systems. However, the use of the luminol-hydrogen peroxide chemiluminescent system is limited by the fact that there are almost no ways of managing the reaction. The introduction of organized molecular systems into the luminol-hydrogen peroxide chemiluminescent system can create an additional channel for controlling chemiluminescent reactions. The luminol-hydrogen peroxide system was not previously studied in various classes of hydrocarbon and perfluorinated micellar solutions. This work was the first to study the effect of cationic, anionic and nonionic hydrocarbon surface-active substances (cetyltrimethylammonium bromide, sodium decyl sulfate, sodium dodecyl sulfate, triton X 100) and perfluorinated surface-active substances (FT-135 and FT-248) on the chemiluminescent systems luminol-hydrogen peroxide-potassium hexacyanoferrate(III) and luminol-hydrogen peroxide-copper(II) sulphate. The systems retain the ability to chemiluminescence in the presence of a surfactant. Cationic surfactants lower the intensity of chemiluminescence, and anionic surfactants increase the intensity of chemiluminescence. The introduction of a surfactant into the system allows increasing the range of dependence of the chemiluminescence intensity on the catalyst concentration. Kinetic curves of the growth and decay of chemiluminescence were measured in the systems. The rate constants of the chemiluminescence decay were measured in the framework of the first-order kinetics model.

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2

Fini, Fabiana, Giorgio Gallinella, Stefano Girotti, Marialuisa Zerbini, and Monica Musiani. "Development of a Chemiluminescence Competitive PCR for the Detection and Quantification of Parvovirus B19 DNA Using a Microplate Luminometer." Clinical Chemistry 45, no. 9 (September 1, 1999): 1391–96. http://dx.doi.org/10.1093/clinchem/45.9.1391.

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Abstract Background: Quantitative PCR of viral nucleic acids can be useful clinically in diagnosis, risk assessment, and monitoring of antiviral therapy. We wished to develop a chemiluminescence competitive PCR (cPCR) for parvovirus B19. Methods: Parvovirus DNA target sequences and competitor sequences were coamplified and directly labeled. Amplified products were then separately hybridized by specific biotin-labeled probes, captured onto streptavidin-coated ELISA microplates, and detected immunoenzymatically using chemiluminescent substrates of peroxidase. Chemiluminescent signals were quantitatively analyzed by a microplate luminometer and were correlated to the amounts of amplified products. Results: Luminol-based systems displayed constant emission but had a higher detection limit (100–1000 genome copies) than the acridan-based system (20 genome copies). The detection limit of chemiluminescent substrates was lower (20 genome copies) than colorimetric substrates (50 genome copies). In chemiluminescence cPCR, the titration curves showed linear correlation above 100 target genome copies. Chemiluminescence cPCR was positive in six serum samples from patients with parvovirus infections and negative in six control sera. Conclusions: The chemiluminescence cPCR appears to be a sensitive and specific method for the quantitative detection of viral DNAs.

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3

Fereja, Tadesse Haile, Ariaya Hymete, and Thirumurugan Gunasekaran. "A Recent Review on Chemiluminescence Reaction, Principle and Application on Pharmaceutical Analysis." ISRN Spectroscopy 2013 (November 26, 2013): 1–12. http://dx.doi.org/10.1155/2013/230858.

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This paper provides a general review on principle of chemiluminescent reactions and their recent applications in drug analysis. The structural requirements for chemiluminescent reactions and the different factors that affect the efficiency of analysis are included in the review. Chemiluminescence application in immunoassay is the new version for this review. Practical considerations are not included in the review since the main interest is to state, through the aforementioned applications, that chemiluminescence has been, is, and will be a versatile tool for pharmaceutical analysis in future years.

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4

Pieńkos, Milena, and Beata Zadykowicz. "Computational Insights on the Mechanism of the Chemiluminescence Reaction of New Group of Chemiluminogens—10-Methyl-9-thiophenoxycarbonylacridinium Cations." International Journal of Molecular Sciences 21, no. 12 (June 21, 2020): 4417. http://dx.doi.org/10.3390/ijms21124417.

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Immunodiagnostics, in which one of the promising procedures is the chemiluminescent labelling, is essential to facilitate the detection of infections in a human organism. One of the standards commonly used in luminometric assays is luminol, which characterized by low quantum yield in aqueous environments. Acridinium esters have better characteristics in this topic. Therefore, the search for new derivatives, especially those characterized by the higher quantum yield of chemiluminescence, is one of the aims of the research undertaken. Using the proposed mechanism of chemiluminescence, we examined the effect of replacing a single atom within a center of reaction on the efficient transformation of substrates into electronically excited products. The density functional theory (DFT) and time dependent (TD) DFT calculated thermodynamic and kinetic data concerning the chemiluminescence and competitive dark pathways suggests that some of the scrutinized derivatives have better characteristics than the chemiluminogens used so far. Synthesis of these candidates for efficient chemiluminogens, followed by studies of their chemiluminescent properties, and ultimately in chemiluminescent labelling, are further steps to confirm their potential applicability in immunodiagnostics.

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5

Kudryashova, A. M., N. A. Mikhailova, and O. V. Borisova. "COMPARISON OF COLORIMETRIC AND CHEMILUMINESCENT ELISA TESTS FOR DETECTION OF IgG ANTIBODIES TO HUMAN EPO IN THE SERA OF EXPERIMENTAL ANIMALS." Medical Immunology (Russia) 20, no. 6 (December 15, 2018): 935–42. http://dx.doi.org/10.15789/1563-0625-2018-6-935-942.

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Production of antibodies to erythropoietin-stimulating drugs is an important problem of therapy with recombinant human erythropoietin (EPO). It leads to changes in the pharmacokinetic profile and decreased therapeutic efficiency. Upon long-term treatment with EPO preparations, neutralizing antibodies can result in rare cases, thus leading to complete pure red cell aplasia. Hence, detection of antibodies to EPO is an important stage in the assessment of the drug immunogenicity in preclinical and clinical studies, as well as during the treatment with EPO. We have compared colorimetric and chemiluminescent ELISA tests for detection of IgG antibodies to human EPO with 3,3’,5,5’-tetramethylbenzidine – hydrogen peroxide and luminol -hydrogen peroxide detection systems, respectively. Аntibodies to human EPO were determined in blood serum samples of experimental animals, i.e., rabbits and guinea pigs following their immunization withdifferent doses of pegylated human recombinant EPO-beta subcutaneously or intravenously. The affinitypurified rabbit polyclonal antibodies to human EPO were used as a reference material. The effects of hydrogen peroxide and luminol concentrations upon sensitivity of a chemiluminescent method were also studied. We have shown a 1.5-2-fold increase in sensitivity when using 4-iodophenol for amplification of chemiluminescence. A comparison of the chemiluminescence intensity with time has demonstrated a better stability for the substrate mixture prepared on borate buffer. A decrease in chemiluminescence signal with time was proportional to the decrease in background signal, thus rendering stability of the signal/background ratio for 3 to 30 minutes. Due to optimizing the substrate mixture composition and conditions of chemiluminescence recording, the reached detection limits for colorimetric and chemiluminescent ELISA’s were, respectively, 0.6 ng/ml and 0.08 ng/ml. The measurement range was extended by more than 20 times for chemiluminescent ELISA. The chemiluminescent ELISA for anti-erythropoietin antibody detection showed a 1.9 to 2.6-fold increase in sensitivity for rabbit serum, and 1.8 to 8.9-fold for guinea pigs serum. Good correlation of results was found for quantitative detection of antibodies in rabbit sera using the two methods (R = 0.981). Thus, chemiluminescent ELISA allowed develop a more sensitive detection technique of IgG antibodies to human erythropoietin.

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6

Gnaim, Samer, Anna Scomparin, Anat Eldar-Boock, Christoph R. Bauer, Ronit Satchi-Fainaro, and Doron Shabat. "Light emission enhancement by supramolecular complexation of chemiluminescence probes designed for bioimaging." Chemical Science 10, no. 10 (2019): 2945–55. http://dx.doi.org/10.1039/c8sc05174g.

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7

Maity, Santanu, Xiaojian Wang, Subhamoy Das, Maomao He, Lee W. Riley, and Niren Murthy. "A cephalosporin–chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude." Chemical Communications 56, no. 24 (2020): 3516–19. http://dx.doi.org/10.1039/c9cc09498a.

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A beta-lactamase chemiluminescent probe, termed CCP, which can for the first time detect beta-lactamase activity via chemiluminescence and 4-orders of magnitude higher than commercial fluorescent probe.

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8

Lin, Ke Li, Tong Yang, Fang Fang Zhang, Gang Lei, Hong Yan Zou, Yuan Fang Li, and Cheng Zhi Huang. "Luminol and gold nanoparticle-co-precipitated reduced graphene oxide hybrids with long-persistent chemiluminescence for cholesterol detection." Journal of Materials Chemistry B 5, no. 35 (2017): 7335–41. http://dx.doi.org/10.1039/c7tb01607g.

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Luminol and AuNP dual-functionalized rGO hybrids (rGO/AuNP/luminol) have been synthesized to generate long-persistent chemiluminescence, which can be used as a chemiluminescent biosensing platform for the detection of cholesterol.

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9

Peck, Evan M., Allen G. Oliver, and Bradley D. Smith. "Enhanced Squaraine Rotaxane Endoperoxide Chemiluminescence in Acidic Alcohols." Australian Journal of Chemistry 68, no. 9 (2015): 1359. http://dx.doi.org/10.1071/ch15196.

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Squaraine rotaxane endoperoxides (SREPs) are storable chemiluminescent compounds that undergo a clean cycloreversion reaction that releases singlet oxygen and emits near-infrared light when warmed to body temperature. This study examined the effect of solvent on SREP chemiluminescence intensity and found that acidic alcohols, such as 2,2,2-trifluoroethanol, α-(trifluoromethyl)benzyl alcohol, and 1,1,1,3,3,3-hexafluoroisopropanol, greatly increased chemiluminescence. In contrast, aprotic solvents, such as trifluoroethylmethyl ether, had no effect. The interlocked rotaxane structure was necessary as no chemiluminescence was observed when the experiments were conducted with samples containing a mixture of the two non-interlocked components (squaraine thread and macrocycle endoperoxide). Spectroscopic analyses of the enhanced SREP chemiluminescent reactions showed a mixture of products. In addition to the expected squaraine rotaxane product caused by cycloreversion of the endoperoxide, a diol derivative was isolated. The results are consistent with an endoperoxide O–O bond cleavage process that is promoted by the hydrogen bonding solvent and produces light emission from a squaraine excited state.

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10

Agatsuma, Shinichi, Toshiyuki Nagoshi, Masaki Kobayashi, Masashi Usa, Haruo Watanabe, Hiroshi Sekino, and Humio Inaba. "Hydroxyl Radical-Induced Characteristic Chemiluminescent Spectra from Plasma of Hemodialysis Patients." Clinical Chemistry 38, no. 1 (January 1, 1992): 48–55. http://dx.doi.org/10.1093/clinchem/38.1.48.

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Abstract Plasma from hemodialysis patients evoked weak photon emissions (chemiluminescence) in a characteristic emission spectrum with a peak at 430 nm, attributed to attack by hydroxyl radicals generated from the iron-catalyzed breakdown of hydrogen peroxide (Fenton reaction), whereas plasma from normal healthy subjects showed a rather weak red chemiluminescence peak at around 680 nm, similar to that resulting from attack by hydroxyl radicals. However, the addition of hydrogen peroxide in the absence of divalent irons induced almost the same red chemiluminescent emission spectrum in both plasmas. The HPLC-gel-filtration chromatography carried out with both plasmas revealed that a primary emitter evoking a peak emission at 430 nm was located in the fraction of lower-molecular-mass substances in fractionated plasma from hemodialysis patients. In contrast, the elution peaks evoking red chemiluminescence with the addition of hydrogen peroxide were mainly observed for the higher-molecular-mass fraction, as determined by gel chromatography of both plasmas. Therefore, the observation of a chemiluminescence peak at 430 nm, induced by the generation of hydroxyl radicals, correlated well with chemiluminescent emissions in plasma samples from patients with chronic renal failure. Spectral analyses of clinical samples that show weak chemiluminescence by forced oxidation by such an active oxygen may provide a new and more sensitive method for diagnosing metabolic disorders.

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11

Selin, A. D., N. A. Terekhina, and G. A. Terekhin. "INFLUENCE OF ELECTROMAGNETIC RADIATION ON THE PERMEABILITY OF ERYTHROCYTE MEMBRANES." Crimea Journal of Experimental and Clinical Medicine 10, no. 4 (2021): 43–49. http://dx.doi.org/10.37279/2224-6444-2020-10-4-43-49.

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The purpose of the survey is to experimentally study the influence of electromagnetic radiation from the decimeter range on the permeability of erythrocyte membranes. The object of the study was the blood of 80 white rats, 60 of them for three months were under the influence of electromagnetic radiation of the decimeter range. 20 animals of the control group were not exposed to electromagnetic fields. The intensity of free radical oxidation was evaluated using chemiluminescent analysis of red blood cells and blood plasma. The permeability of erythrocyte membranes and the content of reduced glutathione in blood erythrocytes were determined spectrophotometrically. A prolonged stay of animals under the influence of an electromagnetic field leads to pronounced changes in chemiluminescence indices in erythrocytes: a decrease in the maximum intensity of chemiluminescence (Imax), light sum (S), and light sum after the maximum value of chemiluminescence (Simax). Despite the increase in the content of glutathione and the chemiluminescent analysis index tg2 reflecting the antioxidant potential, there is an increase in the permeability of erythrocyte membranes under the influence of electromagnetic radiation

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12

Nie, Fei, and Jiuru Lu. "Novel chemiluminescence system with calcein as chemiluminescent reagent." Luminescence 22, no. 5 (2007): 480–86. http://dx.doi.org/10.1002/bio.988.

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13

Sturgess, M. L., I. Weeks, C. N. Mpoko, I. Laing, and J. S. Woodhead. "Chemiluminescent labeled-antibody assay for thyroxin in serum, with magnetic separation of the solid-phase." Clinical Chemistry 32, no. 3 (March 1, 1986): 532–35. http://dx.doi.org/10.1093/clinchem/32.3.532.

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Abstract A chemiluminescent labeled-antibody immunoassay has been developed for measurement of total thyroxin (T4) in serum. Monoclonal antibodies to T4 labeled with a chemiluminescent acridinium ester are used. Serum samples are incubated with the labeled antibodies and a thyroxin-rabbit immunoglobulin conjugate, then reacted with magnetizable particles containing sheep anti-rabbit immunoglobulin. The total reaction time is 40 min. The chemiluminescence intensity of the solid-phase immune complexes is inversely proportional to the concentration of T4 in the sample. The sensitivity of the assay is 1 nmol/L, and the working range of 20-190 nmol/L is characterized by CVs less than or equal to 10%.

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14

Shani, W. Krol, J., Z. Czuba, and S. Scheller. "Modulating Luminol-Dependent Chemiluminescence Of Neutrophils By Flavones." Zeitschrift für Naturforschung C 47, no. 11-12 (December 1, 1992): 889–92. http://dx.doi.org/10.1515/znc-1992-11-1216.

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The effect of 14 flavones on luminol-dependent chemiluminescence of neutrophils was studied in vitro. Chemiluminescence was used in this study as an indicator for the production of a reactive oxygen species by neutrophils, stimulated by phorbol myristate acetate. While flavone- 8-acetic acid, and most of the compounds tested, inhibited chemiluminescence, flavone and its 5-hydroxy-7-methoxy derivatives enhanced it by up to 150%. The most active inhibitors of photon emission were the glycosides. These results indicate that lipophilicity and some structural determinants modulate the chemiluminescent capacity of neutrophils

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15

Pranszke, B., P. Kierzkowski, and A. Kowalski. "A Search for Isotope Effects in Chemiluminescent Reactions of Metastable Ca*( 3Pj, 1D2 ) Atoms with CH3I and CD3I Molecules." Zeitschrift für Naturforschung A 54, no. 3-4 (April 1, 1999): 191–94. http://dx.doi.org/10.1515/zna-1999-3-406.

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Chemiluminescent reactions of calcium atoms in the metastable 3Pj and 1D2 states with CH3I and CD3I were studied in a beam-gas arrangement. Calcium monoiodide spectra associated with transitions from the electronic A 2Π, B 2Σ+ and C 2Π states to the X 2Σ+ ground state were recorded. Total collision and chemiluminescence cross sections were measured. It was found that isotopic substitution in the methyl group does not change the reaction cross sections and the chemiluminescence spectra.

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16

Musiani, M., P. Pasini, M. Zerbini, G. Gentilomi, A. Roda, G. Gallinella, E. Manaresi, and S. Venturoli. "Prenatal Diagnosis of Parvovirus B19-Induced Hydrops Fetalis by Chemiluminescence In Situ Hybridization." Journal of Clinical Microbiology 37, no. 7 (1999): 2326–29. http://dx.doi.org/10.1128/jcm.37.7.2326-2329.1999.

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Parvovirus B19 can be transmitted transplacentally from the infected mother to the fetus during pregnancy, and hydrops fetalis, abortion, or stillbirth can result. In our study we explored the use of chemiluminescence in situ hybridization to detect B19 DNA on cord blood cells, amniotic fluid cells, and pleuric fluid cells from several cases of hydrops fetalis. B19 DNA was detected by using digoxigenin-labeled probes immunoenzymatically visualized with the chemiluminescent adamantil-1,2-dioxetane phenyl phosphate substrate for alkaline phosphatase. The luminescent signal emitted from the hybridized probes was detected, analyzed, and measured with a high-performance, low-light-level imaging luminograph connected to an optical microscope and to a personal computer for the quantification and localization of the chemiluminescent emission inside individual cells.

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17

Rahman, Habibur. "Analytical Applications of Permanganate as an Oxidant in the Determination of Pharmaceuticals Using Chemiluminescence and Spectrophotometry: A Review." Current Analytical Chemistry 16, no. 6 (August 13, 2020): 670–86. http://dx.doi.org/10.2174/1573411015666190617103833.

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Background: Potassium permanganate is a green and versatile industrial oxidizing agent. Due to its high oxidizing ability, it has received considerable attention and has been extensively used for many years for the synthesis, identification, and determination of inorganic and organic compounds. Objective: Potassium permanganate is one of the most applicable oxidants, which has been applied in a number of processes in several industries. Furthermore, it has been widely used in analytical pharmacy to develop analytical methods for pharmaceutically active compounds using chemiluminescence and spectrophotometric techniques. Results: This review covers the importance of potassium permanganate over other common oxidants used in pharmaceuticals and reported its extensive use and analytical applications using direct, indirect and kinetic spectrophotometric methods in different pharmaceutical formulations and biological samples. Chemiluminescent applications of potassium permanganate in the analyses of pharmaceuticals using flow and sequential injection techniques are also discussed. Conclusion: This review summarizes the extensive use of potassium permanganate as a chromogenic and chemiluminescent reagent in the analyses of pharmaceutically active compounds to develop spectrophotometric and chemiluminescence methods since 2000.

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18

Fethi, F., F. Poblete, E. Martinez, A. Gonzalez Urena, and G. Taieb. "Reaction Cross Sections of Ca (41S, 43P and 31D States) With Halogenated Compounds and Water." Laser Chemistry 16, no. 4 (January 1, 1996): 229–43. http://dx.doi.org/10.1155/1996/29359.

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By using two independent and different methods, absolute values of the reaction cross-sections have been determined for the following chemiluminescent reactions Ca(3P,1D)+Cl4C(CHBr3)→CaX*(A, B)(X=Cl, Br)+Cl3C(CHBr2) and Ca(1D)+H2O→CaOH*+H Both chemiluminescence and laser-induced fluorescence spectra are reported. A comparison with related types of reactions is also presented.

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19

SARVER, RONALD, CAYLEY HIGBEE, PREETHA BISWAS, LEI ZHANG, NATE BANNER, JENNIFER RICE, MARK MOZOLA, et al. "A Portable Chemiluminescence Assay of Alkaline Phosphatase Activity to Monitor Pasteurization of Milk Products." Journal of Food Protection 82, no. 12 (November 15, 2019): 2119–25. http://dx.doi.org/10.4315/0362-028x.jfp-19-153.

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ABSTRACT A chemiluminescence assay using a handheld luminometer to measure the activity of alkaline phosphatase was developed that can detect 0.002% or more of unpasteurized milk in various milk products. Evaluation of the assay followed an National Conference on Interstate Milk Shipments (NCIMS)–approved protocol in which aliquots of pasteurized milk products were spiked with raw milk at various levels. Milk products evaluated included skim white milk, 1 and 2% fat content white milk, whole white milk, strawberry-flavored 1% fat content milk, chocolate-flavored 1% fat content milk, half-and-half, and heavy cream. Split samples were prepared, and alkaline phosphatase activities were determined in triplicate on 4 days by three NCIMS-accredited laboratories by the chemiluminescent method and NCIMS-approved reference methods. Equivalence of the chemiluminescent method to the approved reference methods was demonstrated for all eight products evaluated over a range of raw milk concentration from 0 to 0.5%, using criteria established by NCIMS, in which mean results obtained by the three laboratories by the chemiluminescent method were within 1 standard deviation of the mean results obtained by the NCIMS-approved reference methods at each alkaline phosphatase concentration. Procedures for measurement of microbial and reactivated alkaline phosphatase were also established for the method. HIGHLIGHTS

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20

Stanchev, Stancho, Ivanka Pencheva, Spiro Konstantinov, Danka Obreshkova, and Vera Hadjimitova. "Application of UV-Vis spectrophotometric and chemiluminescent methods for evaluation of the antioxidant action of curcumin." Journal of the Serbian Chemical Society 77, no. 8 (2012): 1063–69. http://dx.doi.org/10.2298/jsc110621032s.

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Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5- dione) is a natural biological active substance with an antioxidant activity. The ability of curcumin to inhibit the free radical mechanisms can be used in a prevention of diseases such as cancer and coronary heart disease. The UV-VIS spectrophotometric and chemiluminescent dynamic methods for determination of antioxidant activity of curcumin were developed. The spectrophotometric method includes investigation of the interaction between DNA, isolated from HL-60 cells, and curcumin. The decreasing of the absorption of curcumin in the presence of HL-60 DNA against the blank sample can be a measurement for some complex formation between curcumin and DNA. The chemiluminescent method involves three tests for detection of luminol - depending chemiluminescence on the base of model systems which generate superoxide, hydroxide and hypochlorite radicals. The strongest decay of chemilunimescence was registered at the highest concentration of curcumin (100 ?mol/L).

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21

Choi, Hong Yeob, Jin Hyang Song, Yong Sung Park, Gabriel Lord, and Dong Ki Park. "Flow injection-chemiluminescent assay for the determination of superoxide dismutase activity." Canadian Journal of Chemistry 79, no. 3 (March 1, 2001): 337–41. http://dx.doi.org/10.1139/v01-026.

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Superoxide dismutase (SOD) activity was measured using a flow injection-chemiluminescent assay (FI-CLA) based on xanthinexanthine oxidase dependent superoxide (O2.) formation. The mobile phase consists of 50 mM potassium phosphate buffer (pH 7.4) containing lucigenin (5 µM) and xanthine (0.3 mM). Under optimum conditions, bovine albumin did not affect chemiluminescence at concentrations of 11000 µg mL1 and KCN inhibited 100% of the Cu, Zn-SOD activity using 5 µL of a 0.23 mM concentration. The analysis of one sample was done in less than 30 s with a relative standard deviation of ±3.1%. SOD activity in the biological samples was correlated to the amount of exogenously applied SOD. The FI-CLA method reported here appears to be one of the faster and more useful tools used to assay SOD activity.Key words: superoxide dismutase, flow injection-chemiluminescent assay, superoxide, xanthine oxidase, lucigenin.

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22

Jiang, Qingqing, Fei Nie, and Jiuru Lu. "Chemiluminescence determination of bromhexine hydrochloride with morin as chemiluminescent reagent." Luminescence 23, no. 1 (January 2008): 32–36. http://dx.doi.org/10.1002/bio.1013.

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23

Voicescu, Mariana, Rodica Ion, and Aurelia Meghea. "Evaluation of the oxidative activity of some free base porphyrins by a chemiluminescence method." Journal of the Serbian Chemical Society 75, no. 3 (2010): 333–41. http://dx.doi.org/10.2298/jsc090809021v.

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Due to their spectral characteristics, phototoxicity and high affinity for tumour tissues, porphyrins and their derivatives are widely used in modern medicine as contrast agents for cancer diagnostics and as sensitizers in photodynamic therapy, where they kill tumours via enhancement of tumour oxidative stress. The aim of this work was to simulate in vitro the effects caused by oxidation of two free base porphyrins, 5,10,15,20-tetraphenylporphyrin (TPP) and 5,10,15,20-tetra(4-methoxyphenyl)porphyrin (TMOPP). The kinetic study was monitored using spectral techniques and chemiluminescence. The effect of both porphyrins on an oxidation process was evidenced using the chemiluminescent system, luminal-hydrogen peroxide, in a phosphate buffer at pH 7. It was found that at low concentration, TPP exerts the anti-oxidative effect in the employed chemiluminescent system, while at higher concentrations, its effect is pro-oxidative. TMOPP exerts a pro-oxidant effect, which was more pronounced than TPP. The results are discussed with respect to oxidative stress.

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24

Miller, S. A., M. S. Morton, and A. Turkes. "Chemiluminescence Immunoassay for Progesterone in Plasma Incorporating Acridinium Ester Labelled Antigen." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 25, no. 1 (January 1988): 27–34. http://dx.doi.org/10.1177/000456328802500103.

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A sensitive, solid-phase chemiluminescence immunoassay suitable for determining progesterone concentrations in plasma has been developed. The solid-phase antiserum was prepared by coupling a monoclonal progesterone-antibody, raised against a progesterone-11α-hemisuccinyl/bovine serum albumin conjugate, to cyanogen bromide activated cellulose. An 11α-progesteryl-2-carboxymethyltyramine-4-(10-methyl)-acridinium-9-carboxylate conjugate was used as the chemiluminescent label. The assay had a lower limit of sensitivity of 3 pg/assay tube and satisfied accepted validation criteria. Progesterone concentrations determined by chemiluminescence assay were in good agreement not only with a radioimmunoassay in routine use but also with a gas chromatography-mass spectrometry procedure.

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25

OLINESCU, R. M., MARIA GREABU, D. CROCNAN, F. A. KUMMEROW, VALY CONSTANTINESCU, and FRAGA PAVELIU. "COMPARATIVE STUDY OF THE CHEMILUMINESCENCE PRODUCED BY THE ACTIVATED POLYMORPHONUCLEAR LEUKOCYTES IN PHYSIOLOGICAL AND VARIOUS PATHOLOGICAL CONDITIONS." SOUTHERN BRAZILIAN JOURNAL OF CHEMISTRY 6, no. 6 (June 20, 1998): 25–32. http://dx.doi.org/10.48141/sbjchem.v6.n6.1998.27_1998.pdf.

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The chemiluminescent emission released by activated polymorphonuclear leukocytes has been known for a long time, but its clinical use is still scarce. We suggest the as a quantitative measurement of chemiluminescence, the stimulatmy index (ratio of the chemiluminescence value of stimulated polymorphonuclear leukocytes to non-stimulated leukocytes) was obtained for the same individual. The results obtained by a standardized, simplified technique for more than 2000 individuals (healthy persons such as medical personnel, athletes, soldier, and patients suffering from cardiovascular disease, cancer, and pneumoconiosis) explain the still restricted use of chemiluminescence. There was a wide range of individual variations and an overlap of normal physiological and pathological values. A significant statistical difference was obtained only for patients in the acute phase of the disease. The chemiluminescence measurement of phagocytic activity of leukocytes is strongly influenced by age, diet, presence of stress, and chronic inflammations.

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Singh, A. K., Y. Jiang, T. White, and D. Spassova. "Validation of Nonradioactive Chemiluminescent Immunoassay Methods for the Analysis of Thyroxine and Cortisol in Blood Samples Obtained from Dogs, Cats, and Horses." Journal of Veterinary Diagnostic Investigation 9, no. 3 (July 1997): 261–68. http://dx.doi.org/10.1177/104063879700900307.

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The performances of a radioimmunoassay method, a chemiluminescent immunoassay method, and a chemiluminescent-enzyme immunoassay method were evaluated for the analysis of cortisol and total thyroxine in blood samples obtained from dogs, cats, horses, and humans (reference samples). The analysis of cortisol in human and animal samples exhibited good precision, linearity, and recovery. The 3 methods gave comparable values for the ACTH-induced increase and the dexamethasone-induced decrease in cortisol concentrations in animal samples. The recoveries of total thyroxine from human samples, analyzed by the 3 methods, were comparable. However, the basal total thyroxine concentrations determined by the chemiluminescent immunoassay method were 30–40% lower than those determined by the radioimmunoassay and the chemiluminescent-enzyme immunoassay methods in animal samples. In both human and animal samples, the plot of thyroxine values obtained by the radioimmunoassay method against those obtained by the chemiluminescent immunoassay method or the chemiluminescent-enzyme immunoassay method was linear. However, although the slope of the radioimmunoassay versus chemiluminescent-enzyme immunoassay curve was close to unity, the slope of the radioimmunoassay versus chemiluminescent immunoassay curve was 0.6. This result suggests that, compared with the radioimmunoassay method, the chemiluminescent immunoassay method underestimated thyroxine values in animal samples but not in human samples. Although all 3 methods yielded comparable changes in thyroxine concentrations in response to thyroid stimulating hormone, they did not yield comparable thyroxine concentrations in response to T3 suppression in dogs and cats.

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Fan, F. R. F., A. Mau, and A. J. Bard. "Electrogenerated chemiluminescence, a chemiluminescent polymer based on poly(vinyl-9,10-diphenylanthracene)." Chemical Physics Letters 116, no. 5 (May 1985): 400–404. http://dx.doi.org/10.1016/0009-2614(85)80192-5.

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Phillips, Donald B. "A chemiluminescent sign." Journal of Chemical Education 70, no. 9 (September 1993): 773. http://dx.doi.org/10.1021/ed070p773.

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Akutagawa, Masamoto, Akira Katoh, Hiroshi Yamamoto, Hiromu Aoyama, and Yoshimori Omote. "A chemiluminescent iminehydroperoxide." Journal of the Chemical Society, Chemical Communications, no. 19 (1985): 1290. http://dx.doi.org/10.1039/c39850001290.

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30

Buzanovskii, V. A., and A. A. Bulaev. "Chemiluminescent gas analyzers." Chemical and Petroleum Engineering 44, no. 9-10 (September 2008): 514–18. http://dx.doi.org/10.1007/s10556-008-9093-8.

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31

Marquette, Christophe A., and Loïc J. Blum. "Electro-chemiluminescent biosensing." Analytical and Bioanalytical Chemistry 390, no. 1 (October 2, 2007): 155–68. http://dx.doi.org/10.1007/s00216-007-1631-2.

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32

Tode, B., G. Messeri, F. Bassi, M. Pazzagli, and M. Serio. "Chemiluminescence immunoassay for somatomedin C in serum." Clinical Chemistry 33, no. 11 (November 1, 1987): 1989–93. http://dx.doi.org/10.1093/clinchem/33.11.1989.

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Abstract To select the best tracer for use in a competitive immunoassay, we conjugated human somatomedin C (SmC) to various chemiluminescent compounds via two different synthetic pathways. Naphthylhydrazides and arylhydrazides, used as the labels, were incorporated via their imidate or their succinimide esters. Conjugating the carboxy terminal of (amino ethyl)ethyl-isoluminol to SmC via a succinimide linkage supplied the most sensitive detection limit and the most immunoreactive conjugate. We developed an immunoassay based on the use of this conjugate, and evaluated dextran-coated charcoal, second-antibody precipitation, and solid-phase immunoprecipitation for separating bound and free label. This chemiluminescent method has a detection limit of 16 pg per tube, and it is accurate and precise. Correlation studies with a conventional radioimmunoassay (x) for SmC gave the following regression equation: y = 0.66x + 3.76 (r = 0.953, n = 30); the slight discrepancies between the two methods are probably ascribable to the use of different antibodies. We thus propose this chemiluminescence immunoassay as an inexpensive and sensitive alternative to radioimmunoassay for measuring SmC in serum or in extracts of serum.

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33

Roby, R. J., A. J. Hamer, E. L. Johnson, S. A. Tilstra, and T. J. Burt. "Improved Method for Flame Detection in Combustion Turbines." Journal of Engineering for Gas Turbines and Power 117, no. 2 (April 1, 1995): 332–40. http://dx.doi.org/10.1115/1.2814099.

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A fast response chemiluminescent flame detection approach is presented along with field test results from a fiber optic based flame detector device. Chemiluminescence, the light given off by molecules formed in their excited states, has long been recognized as a diagnostics method for use in combustion. The recent advent of higher quality optical fibers with improved transmission properties in the UV, as well as UV optical detectors, has made the use of chemiluminescence for gas turbine diagnostics and monitoring practical. Advances in combustor designs on new low-emissions machines as well as reliability issues with some existing machines are creating the need for improved flame dynamics measurements as well as improvements in reliability for existing measurements such as combustor flame detection. This paper discusses the technology, principle of operation, and detectors that operate on the chemiluminescence principle.

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Fatoki, Toluwase H. "In Silico Investigation of Luminol, Its Analogues and Mechanism of Chemiluminescence for Blood Identification Beyond Forensics." Current Chemical Biology 14, no. 2 (November 19, 2020): 117–27. http://dx.doi.org/10.2174/2212796814999200801020729.

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Objective: This study aimed at discovering chemiluminescent analogues of luminol, predict their molecular binding to hemoglobin of bloodstains in household crime, and expound the mechanism of chemiluminescence of luminol. Materials and Methods: Similarity and clustering analyses of luminol analogues were conducted, and molecular docking was carried out using hemoglobin from Homo sapiens and four domestic organisms namely Gallus gallus, Drosophila melanogaster, Rattus norvegicus, and Canis familiaris. Results: The results showed the order of overall binding score as D. melanogaster > H. sapiens > C. familiaris > R. norvegicus > G. gallus. Seven compounds namely ZINC16958228, ZINC17023010, ZINC19915427, ZINC34928954, ZINC19915369, ZINC19915444, and ZINC82294978, were found to be consistently stable in binding with diverse hemoglobin and possibly have chemiluminescence than luminol in this in silico study. The interaction of human hemoglobin with luminol and its analogues, showed that amino acid residues His45, Lys61, Asn68, Val73, Met76, Pro77, Ala79, Ala82, Leu83, Pro95, Phe98, Lys99, Ser102, Ser133, Ala134, and Thr134, were possibly significant in the mechanism of action of presumptive test compounds. It was hypothesized that the improved mechanism of chemiluminescent for the identification of blood was based on peroxidase-like reaction, that produces nitric oxide which binds to hemoglobin (Hb) and inhibits Hb degradation without yielding fluorescent products. The compound 2,3-benzodioxine-1,4,5(6H)-trione was formed, which possibly emits light. Conclusion: This study provides novel insight on the luminol and its expanded mechanism for broader possible applications with careful development of new methodologies.

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35

Song, Li, Chunmei Xie, Xueke Liu, Zhen Huo, Yinhai Xie, Jiafeng Gao, Shuping Zhou, Jing Shen, Xiaolong Tang, and Xinkuang Liu. "Development of a Sandwich Chemiluminescence Immunoassay for the Detection of Intact Procollagen Type I N Propeptide with Magnetic Nanosphere Carrier Technology." Journal of Biomedical Nanotechnology 17, no. 8 (August 1, 2021): 1690–98. http://dx.doi.org/10.1166/jbn.2021.3132.

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The metabolic product of type I collagen synthesis, intact procollagen type I N propeptide (intact PINP), is a potential marker of bone formation and osteoporosis, which is not affected by kidney function. We sought to establish a chemiluminescent immunoassay method for the detection of serum intact PINP with previously prepared paired monoclonal antibodies and to evaluate the diagnostic value of the assay in osteoporosis. Using the capture molecule and monoclonal antibody as detection molecule, a diagnostic reagent was developed to detect intact PINP in serum with magnetic nanosphere carriers by the chemiluminescence method, and its analytical performance in the laboratory was evaluated. Serum intact PINP was measured in 142 healthy people and 115 osteoporosis patients. Results were matched with results of a similar test kit, Roche total PINP Elecsys Chemiluminescent Immunoassay Assay. Compared with the performance of the Roche PINP assay product, our method had higher sensitivity (0.02 ng/mL), wider linear range (0.02-1500 ng/mL), and anti-interference. Serum intact PINP values in osteoporosis patients were significantly higher than in healthy subjects (p < 0.001). Our method had good consistency compared with the Roche PINP assay (r = 0.9794). This chemiluminescence method for detecting serum intact PINP (CLIA-intact PINP) with magnetic nanosphere carrier technology meets the requirements of a clinical testing reagent and is expected to have clinical application after further evaluation and can compete with expensive imported kits on the market.

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36

Motoyoshiya, Jiro. "Chemiluminescence in Organic Reactions: Fundamental Investigation and Application of Peroxyoxalate Chemiluminescence and Related Chemiluminescent Reactions." Journal of Synthetic Organic Chemistry, Japan 70, no. 10 (2012): 1018–29. http://dx.doi.org/10.5059/yukigoseikyokaishi.70.1018.

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37

Li, Haigang, and Zhike He. "Magnetic bead-based DNA hybridization assay with chemiluminescence and chemiluminescent imaging detection." Analyst 134, no. 4 (2009): 800. http://dx.doi.org/10.1039/b819990f.

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38

Zhao, Shulin, Yong Huang, Rongjun Liu, Ming Shi, and Yi-Ming Liu. "A Nonenzymatic Chemiluminescent Reaction Enabling Chemiluminescence Resonance Energy Transfer to Quantum Dots." Chemistry - A European Journal 16, no. 21 (April 21, 2010): 6142–45. http://dx.doi.org/10.1002/chem.201000478.

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39

Blazheyevskіy, M. Ye, O. V. Koval’ska, and K. V. Dynnyk. "A novel luminol-based chemiluminescence method for detecting acetylcholine." Journal of Organic and Pharmaceutical Chemistry 19, no. 1(73) (March 15, 2021): 25–31. http://dx.doi.org/10.24959/ophcj.21.224212.

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Aim. To develop а new simple non-enzymatic method for the determination of acetylcholine (ACh) by the chemiluminescent reaction of luminol under conditions of the enzymatic hydrolysis of acetylcholine (pH 8.5).Experimental part. The method proposed is based on the perhydrolysis reaction of ACh by the excess ofhydrogen peroxide with the formation of peracetic acid. The latter was further determined by the activation eﬀect of the luminol chemiluminescent oxidation reaction in the presence of hydrogen peroxide. The analytical signal was the summary luminescence (Σ) registered within certain time.Results and discussion. The pH range of the analytically applicable system was from 8.2 to 8.5. The eﬀect of ACh + H2O2 incubation period on the reaction progress was also studied. The increase of the incubation period enhanced the sensitivity of the method (the limit of detection (LOD)), but because of practical reasons (especially the detection speed) and practical experience the incubation period was set to 30 min. The linear dependence was observed in the acetylcholine chloride concentration range of (0.8 – 2.8) × 10-4 mol/L. While determining acetylcholine chloride in the concentration range of (1.1 – 2.2) × 10-4 mol/L the relative standard deviation (RSD) did notexceed 3 % ((X – μ) × 100 %/μ = –0.5…+0.5 %). The Limit of Quantitation (LOQ, 10S) was 7.7 × 10-5 mol/L.Conclusions. A new non-enzymatic kinetic method for the chemiluminescent determination of ACh in aqueous solutions and the pharmaceutical formulation Acetylcholinchlorid Injeel® has been proposed. This method is simple, fast, inexpensive, and thus appropriate for the routine ACh quality control in the laboratories of hospitals, pharmaceutical industries and research institutions.Key words: acetylcholine; chemiluminescence method

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40

Yappert, M. Cecilia, and J. D. Ingle. "Absorption-Corrected Spectral Studies of the Lucigenin Chemiluminescence Reaction." Applied Spectroscopy 43, no. 5 (July 1989): 767–71. http://dx.doi.org/10.1366/0003702894202111.

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With a spectrometer based on two multichannel detectors, luminescence and absorption spectra are acquired simultaneously during the reaction between lucigenin and H2O2 in a basic solution. Alterations in the fluorescence and chemiluminescence spectral contours occur during the reaction, due to time-dependent inner filter effects caused by the changing absorption of the emission radiation by reactants, intermediates, or products. The spectrometer uses the measured absorbances and appropriate equations for automatic correction of luminescence spectra for inner filter effects. The corrected spectra demonstrate that the primary fluorescent product of the reaction, N-methyl acridone, is not the primary chemiluminescent emitting species.

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41

Sushma, Umesh, Alok K. Srivastava, and Manonmani H. Krishnan. "Melamine Detection in Food matrices employing Chicken Antibody (IgY): A Comparison between Colorimetric and Chemiluminescent Methods." Current Analytical Chemistry 15, no. 6 (October 3, 2019): 668–77. http://dx.doi.org/10.2174/1573411015666181205120323.

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Background:Melamine, contains 67% nitrogen by mass, and is adulterated in foods to uplift false protein. There is an urgent requirement to develop fast screening techniques for monitoring melamine in foods.Objective:To develop rapid, high throughput detection techniques for melamine in the food matrix.Methods:IgY antibodies were developed against melamine in the hen, isolated and used for detection of melamine. The detection by colorimetric and chemiluminescent methods was compared.Results:The detection range for melamine was 1 ng-25 µg by the colorimetric method and 10 fg/mL-25 ng/mL by the chemiluminescent method. There was a very low matrix effect, where the recovery was 86 to 106 % by colorimetric method and 71 to 98 % by the chemiluminescent method.Conclusion:Both colorimetric and chemiluminescent methods could be employed for the fast and consistent melamine detection in the food matrix.

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42

Israël, M. "A chemiluminescent serotonin assay." Neurochemistry International 42, no. 3 (February 2003): 215–20. http://dx.doi.org/10.1016/s0197-0186(02)00095-5.

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43

Thomas, Nicholas C. "A chemiluminescent ammonia fountain." Journal of Chemical Education 67, no. 4 (April 1990): 339. http://dx.doi.org/10.1021/ed067p339.

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44

Kricka, L. J. "Chemiluminescent and bioluminescent techniques." Clinical Chemistry 37, no. 9 (September 1, 1991): 1472–81. http://dx.doi.org/10.1093/clinchem/37.9.1472.

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Abstract Light-emitting chemical reactions (chemiluminescence, CL) and biological reactions (bioluminescence, BL) have a diverse range of analytical applications but relatively few have been adopted by routine clinical laboratories. Advantages of CL and BL assays include sensitivity (attomole and sub-attomole detection limits), speed (signal generated in a few seconds and in some cases stable for several hours), nonhazardous reagents, and simple procedures. The most promising clinical applications are in immunoassay, protein blotting, and DNA probe assays. Chemiluminescent molecules exploited as labels include luminol, isoluminol, acridinium esters, thioesters and sulfonamides, and phenanthridinium esters. Separation and nonseparation assays have been devised, based on isoluminol and acridinium ester labels. The combination of the amplification properties of an enzyme and a CL or BL detection reaction provides a highly sensitive analytical system. Since 1983, CL and BL methods have been developed for many enzyme labels, e.g., alkaline phosphatase, glucose-6-phosphate dehydrogenase, horseradish peroxidase, Renilla luciferase, and xanthine oxidase. Currently, the most successful enzyme assays are the enhanced CL method for a peroxidase label involving a mixture of luminol, hydrogen peroxide, and an enhancer (e.g., p-iodophenol) and the direct CL method for alkaline phosphatase, with an adamantyl 1,2-dioxetane phenyl phosphate as substrate. Both systems are very sensitive (the detection limit for alkaline phosphatase when using the dioxetane reagent is 0.001 amol) and produce long-lived light emission (greater than 30 min), which is ideal for membrane applications in which light emission is detected with photographic film or a charge-coupled device camera.

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45

Gillevet, Patrick M. "Chemiluminescent multiplex DNA sequencing." Nature 348, no. 6302 (December 1990): 657–58. http://dx.doi.org/10.1038/348657a0.

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46

Stevenson, James D., Anja Dietel, and Neil R. Thomas. "A chemiluminescent catalytic antibody†." Chemical Communications, no. 20 (1999): 2105–6. http://dx.doi.org/10.1039/a906566k.

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47

Kricka, L. J., and T. P. Whitehead. "Chemiluminescent and bioluminescent immunoassays." Journal of Pharmaceutical and Biomedical Analysis 5, no. 8 (January 1987): 829–33. http://dx.doi.org/10.1016/0731-7085(87)80101-2.

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48

Kricka, L. J., and G. H. G. Thorpe. "Enhanced chemiluminescent enzyme immunoassays." Parasitology Today 2, no. 4 (April 1986): 123–25. http://dx.doi.org/10.1016/0169-4758(86)90047-5.

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49

van der Ende, A., R. W. M. van der Hulst, P. Roorda, G. N. J. Tytgat, and J. Dankert. "Evaluation of Three Commercial Serological Tests with Different Methodologies To Assess Helicobacter pyloriInfection." Journal of Clinical Microbiology 37, no. 12 (1999): 4150–52. http://dx.doi.org/10.1128/jcm.37.12.4150-4152.1999.

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The sera of 142 Helicobacter pylori-positive and 32H. pylori-negative patients were assessed by a desktop test (QuickVue), an enzyme-linked immunosorbent assay (ELISA) (HM-CAP), and a solid-phase, two-step chemiluminescent enzyme immunoassay (Immulite). These tests yielded sensitivities of 97, 97, and 91% and specificities of 97, 94, and 100%, respectively. In conclusion, the desktop test and the ELISA are more sensitive than the chemiluminescent enzyme immunoassay (P < 0.05). The chemiluminescent enzyme immunoassay has the advantage that it is fully automated.

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Schank, Kurt, Horst Beck, and Frank Werner. "Chemiluminescent and Non-Chemiluminescent Ozonations of Selected Electron-Rich Alkynes in Halomethanes." Helvetica Chimica Acta 83, no. 7 (July 5, 2000): 1611–24. http://dx.doi.org/10.1002/1522-2675(20000705)83:7<1611::aid-hlca1611>3.0.co;2-l.

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