Journal articles: 'Chemiluminescence'

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Ibragimova, D. A., O. M. Kamil, T. V. Yankova, N. A. Yashtulov, and N. K. Zaitsev. "THE EFFECT OF SURFACTANTS ON THE CHEMILUMINESCENT REACTION OF LUMINOL WITH HYDROGEN PEROXIDE." Fine Chemical Technologies 12, no. 6 (December 28, 2017): 71–76. http://dx.doi.org/10.32362/2410-6593-2017-12-6-71-76.

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The luminol-hydrogen peroxide chemiluminescent system is widely used for the creation of diagnostic systems, for chemical analysis, for studying the kinetics and mechanisms of chemical reactions, for the creation of special and emergency light sources, and for monitoring living systems. However, the use of the luminol-hydrogen peroxide chemiluminescent system is limited by the fact that there are almost no ways of managing the reaction. The introduction of organized molecular systems into the luminol-hydrogen peroxide chemiluminescent system can create an additional channel for controlling chemiluminescent reactions. The luminol-hydrogen peroxide system was not previously studied in various classes of hydrocarbon and perfluorinated micellar solutions. This work was the first to study the effect of cationic, anionic and nonionic hydrocarbon surface-active substances (cetyltrimethylammonium bromide, sodium decyl sulfate, sodium dodecyl sulfate, triton X 100) and perfluorinated surface-active substances (FT-135 and FT-248) on the chemiluminescent systems luminol-hydrogen peroxide-potassium hexacyanoferrate(III) and luminol-hydrogen peroxide-copper(II) sulphate. The systems retain the ability to chemiluminescence in the presence of a surfactant. Cationic surfactants lower the intensity of chemiluminescence, and anionic surfactants increase the intensity of chemiluminescence. The introduction of a surfactant into the system allows increasing the range of dependence of the chemiluminescence intensity on the catalyst concentration. Kinetic curves of the growth and decay of chemiluminescence were measured in the systems. The rate constants of the chemiluminescence decay were measured in the framework of the first-order kinetics model.

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Fini, Fabiana, Giorgio Gallinella, Stefano Girotti, Marialuisa Zerbini, and Monica Musiani. "Development of a Chemiluminescence Competitive PCR for the Detection and Quantification of Parvovirus B19 DNA Using a Microplate Luminometer." Clinical Chemistry 45, no. 9 (September 1, 1999): 1391–96. http://dx.doi.org/10.1093/clinchem/45.9.1391.

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Abstract Background: Quantitative PCR of viral nucleic acids can be useful clinically in diagnosis, risk assessment, and monitoring of antiviral therapy. We wished to develop a chemiluminescence competitive PCR (cPCR) for parvovirus B19. Methods: Parvovirus DNA target sequences and competitor sequences were coamplified and directly labeled. Amplified products were then separately hybridized by specific biotin-labeled probes, captured onto streptavidin-coated ELISA microplates, and detected immunoenzymatically using chemiluminescent substrates of peroxidase. Chemiluminescent signals were quantitatively analyzed by a microplate luminometer and were correlated to the amounts of amplified products. Results: Luminol-based systems displayed constant emission but had a higher detection limit (100–1000 genome copies) than the acridan-based system (20 genome copies). The detection limit of chemiluminescent substrates was lower (20 genome copies) than colorimetric substrates (50 genome copies). In chemiluminescence cPCR, the titration curves showed linear correlation above 100 target genome copies. Chemiluminescence cPCR was positive in six serum samples from patients with parvovirus infections and negative in six control sera. Conclusions: The chemiluminescence cPCR appears to be a sensitive and specific method for the quantitative detection of viral DNAs.

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Fereja, Tadesse Haile, Ariaya Hymete, and Thirumurugan Gunasekaran. "A Recent Review on Chemiluminescence Reaction, Principle and Application on Pharmaceutical Analysis." ISRN Spectroscopy 2013 (November 26, 2013): 1–12. http://dx.doi.org/10.1155/2013/230858.

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This paper provides a general review on principle of chemiluminescent reactions and their recent applications in drug analysis. The structural requirements for chemiluminescent reactions and the different factors that affect the efficiency of analysis are included in the review. Chemiluminescence application in immunoassay is the new version for this review. Practical considerations are not included in the review since the main interest is to state, through the aforementioned applications, that chemiluminescence has been, is, and will be a versatile tool for pharmaceutical analysis in future years.

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Smirnova, O. V., A. A. Sinyakov, and V. V. Tsukanov. "Monocyte Chemiluminescence Traits in Gastric Cancer." Russian Journal of Gastroenterology, Hepatology, Coloproctology 31, no. 2 (June 2, 2021): 34–39. http://dx.doi.org/10.22416/1382-4376-2021-31-2-34-39.

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Aim. A study of monocyte chemiluminescent activity at variant stages of gastric cancer.Materials and methods. The study enrolled 90 gastric cancer patients and 70 healthy donors. Spontaneous and induced chemiluminescence in monocytes was assessed for 90 min with a “BLM 3607” 36-channel chemiluminescence analyser (Russia). Opsonized zymosan-induced chemiluminescence enhancement was measured as a ratio of the areas under the induced vs. spontaneous chemiluminescence curves, the activation index. Statistical significance was estimated with the Mann—Whitney criterion (p < 0.05).Results. The maximal spontaneous monocyte chemiluminescence intensity significantly decreased in stage IV gastric cancer patients compared to the control cohort (p = 0.035). Time to maximum in spontaneous chemiluminescence increased in all gastric cancer patients vs. control (p = 0.001), and in stage IV gastric cancer vs. stage I patients (p = 0.043). The areas under a curve in spontaneous and induced monocyte chemiluminescence increased in all gastric cancer patients vs. control (p = 0.001), and in stage IV gastric cancer vs. stage I patients (p = 0.037). The activation index was higher in all gastric cancer cases compared to control (p = 0.001).Conclusion. All patients with gastric adenocarcinoma, irrespective of the stage, revealed changes in the monocyte chemiluminescence activity, i.e. a longer time to maximum in spontaneous chemiluminescence and larger area under the curve of spontaneous and induced chemiluminescence, the activation index. Maximal monocyte spontaneous chemiluminescence intensity diminished in stage IV gastric cancer compared to the control cohort. Immune activity reflected in monocyte chemiluminescence correlates with the stage of gastric adenocarcinoma.

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Shani, W. Krol, J., Z. Czuba, and S. Scheller. "Modulating Luminol-Dependent Chemiluminescence Of Neutrophils By Flavones." Zeitschrift für Naturforschung C 47, no. 11-12 (December 1, 1992): 889–92. http://dx.doi.org/10.1515/znc-1992-11-1216.

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The effect of 14 flavones on luminol-dependent chemiluminescence of neutrophils was studied in vitro. Chemiluminescence was used in this study as an indicator for the production of a reactive oxygen species by neutrophils, stimulated by phorbol myristate acetate. While flavone- 8-acetic acid, and most of the compounds tested, inhibited chemiluminescence, flavone and its 5-hydroxy-7-methoxy derivatives enhanced it by up to 150%. The most active inhibitors of photon emission were the glycosides. These results indicate that lipophilicity and some structural determinants modulate the chemiluminescent capacity of neutrophils

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Pieńkos, Milena, and Beata Zadykowicz. "Computational Insights on the Mechanism of the Chemiluminescence Reaction of New Group of Chemiluminogens—10-Methyl-9-thiophenoxycarbonylacridinium Cations." International Journal of Molecular Sciences 21, no. 12 (June 21, 2020): 4417. http://dx.doi.org/10.3390/ijms21124417.

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Immunodiagnostics, in which one of the promising procedures is the chemiluminescent labelling, is essential to facilitate the detection of infections in a human organism. One of the standards commonly used in luminometric assays is luminol, which characterized by low quantum yield in aqueous environments. Acridinium esters have better characteristics in this topic. Therefore, the search for new derivatives, especially those characterized by the higher quantum yield of chemiluminescence, is one of the aims of the research undertaken. Using the proposed mechanism of chemiluminescence, we examined the effect of replacing a single atom within a center of reaction on the efficient transformation of substrates into electronically excited products. The density functional theory (DFT) and time dependent (TD) DFT calculated thermodynamic and kinetic data concerning the chemiluminescence and competitive dark pathways suggests that some of the scrutinized derivatives have better characteristics than the chemiluminogens used so far. Synthesis of these candidates for efficient chemiluminogens, followed by studies of their chemiluminescent properties, and ultimately in chemiluminescent labelling, are further steps to confirm their potential applicability in immunodiagnostics.

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Agatsuma, Shinichi, Toshiyuki Nagoshi, Masaki Kobayashi, Masashi Usa, Haruo Watanabe, Hiroshi Sekino, and Humio Inaba. "Hydroxyl Radical-Induced Characteristic Chemiluminescent Spectra from Plasma of Hemodialysis Patients." Clinical Chemistry 38, no. 1 (January 1, 1992): 48–55. http://dx.doi.org/10.1093/clinchem/38.1.48.

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Abstract Plasma from hemodialysis patients evoked weak photon emissions (chemiluminescence) in a characteristic emission spectrum with a peak at 430 nm, attributed to attack by hydroxyl radicals generated from the iron-catalyzed breakdown of hydrogen peroxide (Fenton reaction), whereas plasma from normal healthy subjects showed a rather weak red chemiluminescence peak at around 680 nm, similar to that resulting from attack by hydroxyl radicals. However, the addition of hydrogen peroxide in the absence of divalent irons induced almost the same red chemiluminescent emission spectrum in both plasmas. The HPLC-gel-filtration chromatography carried out with both plasmas revealed that a primary emitter evoking a peak emission at 430 nm was located in the fraction of lower-molecular-mass substances in fractionated plasma from hemodialysis patients. In contrast, the elution peaks evoking red chemiluminescence with the addition of hydrogen peroxide were mainly observed for the higher-molecular-mass fraction, as determined by gel chromatography of both plasmas. Therefore, the observation of a chemiluminescence peak at 430 nm, induced by the generation of hydroxyl radicals, correlated well with chemiluminescent emissions in plasma samples from patients with chronic renal failure. Spectral analyses of clinical samples that show weak chemiluminescence by forced oxidation by such an active oxygen may provide a new and more sensitive method for diagnosing metabolic disorders.

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Kurtasova, I. M., T. V. Lubnina, and E. V. Safontseva. "Сhanges in functional activity of peripheral blood neutrophils in young children with recurrent respiratory infection." Medical Immunology (Russia) 24, no. 2 (April 20, 2022): 407–12. http://dx.doi.org/10.15789/1563-0625-cif-2407.

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Neutrophils characterized by high mobility and ability for quick and accurate reresponse upon homeostatic changes. These changes primarily occur at the inflammation site. The pathogen elimination depends on the phagocytic activity of neutrophils. The data from past decades have revisited the role of neutrophils and their involvement in changing the human cellular and humoral immunity. Neutrophils are not only effector cells, but also regulatory cells of both innate and adaptive immunity. Our purpose was to study phagocytic activity and parameters of oxygen-dependent metabolism of peripheral blood neutrophils in young children with recurrent respiratory infections. We examined 111 children aged 1-3 years with recurrent respiratory infections over the period of clinical remission. The control group consisted of 24 healthy children aged 1-3 years. Phagocytic activity of peripheral blood neutrophils was studied by the latex test. Luminol-dependent chemiluminescence of blood neutrophils was studied according to de Sole et al. (1983). The study of phagocytic indexes of peripheral blood neutrophils in the children with recurrent respiratory infections has revealed a decrease in the number of actively phagocytizing cells and preservation of their absorptive capacity. Studies of luminol-dependent chemiluminescence in peripheral blood neutrophils in children with recurrent respiratory infections revealed changes in oxygen-dependent metabolism depending on the clinical variant of complicated infection. In the group of children with broncho-obstructive syndrome, the background chemiluminescence parameters of peripheral blood neutrophils were characterized by faster time to chemiluminescence curve peak. Chemiluminescence indices induced by opsonized zymosan showed a lower time of reaction to stimuli, decreased intensity of “respiratory burst”-associated luminescence, and decreased trend for activation index of peripheral blood neutrophils. Study of luminol-dependent chemiluminescence peripheral in blood neutrophils in children with hypertrophy of pharyngeal tonsils did not reveal changes in the background chemiluminescence levels. Chemiluminescence evaluation upon stimulation of peripheral blood neutrophils by opsonized zymosan caused a decrease in the stimulated response time, lower maximal “respiratory burst”, and decrease in AUC chemiluminescence. Thus, kinetics of spontaneous chemiluminescent response in peripheral blood neutrophils is impaired.in children with broncho-obstructive syndrome. Similarly, the in vitro neutrophil stimulation showed changes in chemiluminescent response kinetics and decreased reserve of oxygen-dependent metabolic capacity. In the children with hypertrophy of pharyngeal tonsil, we observed changes in chemiluminescent response of peripheral blood neutrophils only after opsonized zymosan induction. Compensatory metabolic capacity of peripheral blood neutrophils was retained in the opsonized zymosan stress tests. The study results showed unidirectional changes in peripheral blood neutrophil phagocytic activity parameters both in children with broncho-obstructive syndrome, and in children with pharyngeal tonsil hypertrophy and recurrent respiratory infection.

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Gnaim, Samer, Anna Scomparin, Anat Eldar-Boock, Christoph R. Bauer, Ronit Satchi-Fainaro, and Doron Shabat. "Light emission enhancement by supramolecular complexation of chemiluminescence probes designed for bioimaging." Chemical Science 10, no. 10 (2019): 2945–55. http://dx.doi.org/10.1039/c8sc05174g.

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Peck, Evan M., Allen G. Oliver, and Bradley D. Smith. "Enhanced Squaraine Rotaxane Endoperoxide Chemiluminescence in Acidic Alcohols." Australian Journal of Chemistry 68, no. 9 (2015): 1359. http://dx.doi.org/10.1071/ch15196.

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Squaraine rotaxane endoperoxides (SREPs) are storable chemiluminescent compounds that undergo a clean cycloreversion reaction that releases singlet oxygen and emits near-infrared light when warmed to body temperature. This study examined the effect of solvent on SREP chemiluminescence intensity and found that acidic alcohols, such as 2,2,2-trifluoroethanol, α-(trifluoromethyl)benzyl alcohol, and 1,1,1,3,3,3-hexafluoroisopropanol, greatly increased chemiluminescence. In contrast, aprotic solvents, such as trifluoroethylmethyl ether, had no effect. The interlocked rotaxane structure was necessary as no chemiluminescence was observed when the experiments were conducted with samples containing a mixture of the two non-interlocked components (squaraine thread and macrocycle endoperoxide). Spectroscopic analyses of the enhanced SREP chemiluminescent reactions showed a mixture of products. In addition to the expected squaraine rotaxane product caused by cycloreversion of the endoperoxide, a diol derivative was isolated. The results are consistent with an endoperoxide O–O bond cleavage process that is promoted by the hydrogen bonding solvent and produces light emission from a squaraine excited state.

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Lekomtseva, Y. V. "THE INCREASED KINETICS OF H2O2-INDUCED CHEMILUMINESCENCE IN THE PATIENTS WITH LONG-TERM CONSEQUENCES AFTER CEREBRAL CONTUSION." Modern medical technologies 43, no. 4 (December 2019): 21–25. http://dx.doi.org/10.34287/mmt.4(43).2019.4.

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Abstract Purpose of the study. The aim was to study in the patients with long-term consequences after cerebral contusion the intensity of spontaneous and H2O2-induced chemiluminescence in order to evaluate the early fast-flowing reactions caused by oxidative stress and associated with the formation of primary radicals such as free radical oxidations. Materials and Methods. Forty-two patients with long-term consequences after cerebral contusion were investigated (39,04 ± 12,84 years mean age; mean onset years 32,56 ± 6,4) where both spontaneous and H2O2-induced chemiluminescences were measured directly by HPLC-chemiluminescence assay. Results. The study have showed that sera of the investigated patients with long-term consequences after cerebral contusion have the increased H2O2-induced chemiluminescence associated with the high amplitude of «fast» burst and the tendency to increase of spontaneous chemiluminescence (p = 0,039 and p = 0,58, accordingly). Thus, the patients with long-term consequences after cerebral contusion showed the abnormal high kinetics of H2O2-induced chemiluminescence (p < 0,05). The statistically significant increase serum Н2О2-induced chemiluminescence intensity detected in examined patients (3085,6 ± 114,2 vs 669,1 ± 214,83 controls) have showed the development of certain oxidative stress processes in this category of patients associated with the increasing of primary free radical reactions and their activity were getting increased with the progression of the disease duration (p < 0,05). Conclusions. The study provides the novel data revealing the increased kinetics of H2O2-induced chemiluminescence in the patients with long-term consequences after cerebral contusion accompanied by the tendency to increase of spontaneous chemiluminescence that may play the certain pathogenetic role. Keywords: H2O2-induced chemiluminescence, long-term consequences after cerebral contusion, oxidative stress.

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OLINESCU, R. M., MARIA GREABU, D. CROCNAN, F. A. KUMMEROW, VALY CONSTANTINESCU, and FRAGA PAVELIU. "COMPARATIVE STUDY OF THE CHEMILUMINESCENCE PRODUCED BY THE ACTIVATED POLYMORPHONUCLEAR LEUKOCYTES IN PHYSIOLOGICAL AND VARIOUS PATHOLOGICAL CONDITIONS." SOUTHERN BRAZILIAN JOURNAL OF CHEMISTRY 6, no. 6 (June 20, 1998): 25–32. http://dx.doi.org/10.48141/sbjchem.v6.n6.1998.27_1998.pdf.

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The chemiluminescent emission released by activated polymorphonuclear leukocytes has been known for a long time, but its clinical use is still scarce. We suggest the as a quantitative measurement of chemiluminescence, the stimulatmy index (ratio of the chemiluminescence value of stimulated polymorphonuclear leukocytes to non-stimulated leukocytes) was obtained for the same individual. The results obtained by a standardized, simplified technique for more than 2000 individuals (healthy persons such as medical personnel, athletes, soldier, and patients suffering from cardiovascular disease, cancer, and pneumoconiosis) explain the still restricted use of chemiluminescence. There was a wide range of individual variations and an overlap of normal physiological and pathological values. A significant statistical difference was obtained only for patients in the acute phase of the disease. The chemiluminescence measurement of phagocytic activity of leukocytes is strongly influenced by age, diet, presence of stress, and chronic inflammations.

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Selin, A. D., N. A. Terekhina, and G. A. Terekhin. "INFLUENCE OF ELECTROMAGNETIC RADIATION ON THE PERMEABILITY OF ERYTHROCYTE MEMBRANES." Crimea Journal of Experimental and Clinical Medicine 10, no. 4 (2021): 43–49. http://dx.doi.org/10.37279/2224-6444-2020-10-4-43-49.

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The purpose of the survey is to experimentally study the influence of electromagnetic radiation from the decimeter range on the permeability of erythrocyte membranes. The object of the study was the blood of 80 white rats, 60 of them for three months were under the influence of electromagnetic radiation of the decimeter range. 20 animals of the control group were not exposed to electromagnetic fields. The intensity of free radical oxidation was evaluated using chemiluminescent analysis of red blood cells and blood plasma. The permeability of erythrocyte membranes and the content of reduced glutathione in blood erythrocytes were determined spectrophotometrically. A prolonged stay of animals under the influence of an electromagnetic field leads to pronounced changes in chemiluminescence indices in erythrocytes: a decrease in the maximum intensity of chemiluminescence (Imax), light sum (S), and light sum after the maximum value of chemiluminescence (Simax). Despite the increase in the content of glutathione and the chemiluminescent analysis index tg2 reflecting the antioxidant potential, there is an increase in the permeability of erythrocyte membranes under the influence of electromagnetic radiation

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Pranszke, B., P. Kierzkowski, and A. Kowalski. "A Search for Isotope Effects in Chemiluminescent Reactions of Metastable Ca*( 3Pj, 1D2 ) Atoms with CH3I and CD3I Molecules." Zeitschrift für Naturforschung A 54, no. 3-4 (April 1, 1999): 191–94. http://dx.doi.org/10.1515/zna-1999-3-406.

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Chemiluminescent reactions of calcium atoms in the metastable 3Pj and 1D2 states with CH3I and CD3I were studied in a beam-gas arrangement. Calcium monoiodide spectra associated with transitions from the electronic A 2Π, B 2Σ+ and C 2Π states to the X 2Σ+ ground state were recorded. Total collision and chemiluminescence cross sections were measured. It was found that isotopic substitution in the methyl group does not change the reaction cross sections and the chemiluminescence spectra.

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Smirnova, O. V., E. V. Kasparov, Ya I. Perepechay, A. A. Nesytykh, and V. S. Belyaev. "FEATURES OF NEUTROPHIL CHEMILUMINESCENCE IN THE PATIENTS WITH ADVANCED RECTAL CANCER." Medical Immunology (Russia) 21, no. 1 (January 24, 2019): 157–64. http://dx.doi.org/10.15789/1563-0625-2019-1-157-164.

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Colorectal cancer is one of the most common malignant diseases in Russia worldwide making up 5-6% of all human malignant tumors. Neutrophilic granulocytes are actively involved in development of antitumor response. A key role in tumor regression is assigned to active forms of oxygen produced by neutrophils. In connection with these pre-requisites, our goal was to study functional characteristics of spontaneous and induced chemiluminescent activity of neutrophil granulocytes in patients with rectal cancer before starting pathogenetic therapy and in subsequent dynamics. The paper presents some laboratory results, i.e., functional indices of neutrophilic granulocytes’ activity in 36 patients with rectal cancer being at different stages of oncological process. The control group consisted of 112 practically healthy volunteers, comparable in sex and age to the group of patients under study. To perform the study venous blood was taken from patients to vacuum test tubes with lithium heparin in the morning time before surgical treatment, and on day 7 after the surgical intervention. Evaluation of spontaneous and induced chemiluminescence was performed for 90 minutes in a 36-channel “CL 3606” chemiluminescence analyzer (Russia). The following characteristics were determined: time of the curve transition to maximal chemiluminescence intensity (Tmax), maximal value of chemiluminescence intensity (Imax), integral area under the chemiluminescence curve (S). Luminol was used as the chemiluminescence enhancer. Opsonized zymosan was used to induce the respiratory explosion. Chemiluminescence amplification induced by opsonized zymosan was evaluated by the ratio of induced-tospontaneous chemiluminescence (Sind/spont) designated as an activation index.Analysis of chemiluminescence activity in neutrophilic granulocytes showed a significant increase in spontaneous chemiluminescence activity at the stages III and IV of the disease. The production of active oxygen forms induced in neutrophilic granulocytes by opsonized zymosan increased in all the study groups, relative to control parameters. The area under the curves of spontaneous and induced chemiluminescence in patients with colorectal cancer at all stages of the oncological process is less, as compared to the control group, which, despite high indices of maximal chemiluminescence activity, may indicate insufficient total production of reactive oxygen species. The time-to-peak values of the chemiluminescence curves in patients with rectal cancer at all stages of the disease did not show statistically significant differences from the control group.

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Kudryashova, A. M., N. A. Mikhailova, and O. V. Borisova. "COMPARISON OF COLORIMETRIC AND CHEMILUMINESCENT ELISA TESTS FOR DETECTION OF IgG ANTIBODIES TO HUMAN EPO IN THE SERA OF EXPERIMENTAL ANIMALS." Medical Immunology (Russia) 20, no. 6 (December 15, 2018): 935–42. http://dx.doi.org/10.15789/1563-0625-2018-6-935-942.

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Production of antibodies to erythropoietin-stimulating drugs is an important problem of therapy with recombinant human erythropoietin (EPO). It leads to changes in the pharmacokinetic profile and decreased therapeutic efficiency. Upon long-term treatment with EPO preparations, neutralizing antibodies can result in rare cases, thus leading to complete pure red cell aplasia. Hence, detection of antibodies to EPO is an important stage in the assessment of the drug immunogenicity in preclinical and clinical studies, as well as during the treatment with EPO. We have compared colorimetric and chemiluminescent ELISA tests for detection of IgG antibodies to human EPO with 3,3’,5,5’-tetramethylbenzidine – hydrogen peroxide and luminol -hydrogen peroxide detection systems, respectively. Аntibodies to human EPO were determined in blood serum samples of experimental animals, i.e., rabbits and guinea pigs following their immunization withdifferent doses of pegylated human recombinant EPO-beta subcutaneously or intravenously. The affinitypurified rabbit polyclonal antibodies to human EPO were used as a reference material. The effects of hydrogen peroxide and luminol concentrations upon sensitivity of a chemiluminescent method were also studied. We have shown a 1.5-2-fold increase in sensitivity when using 4-iodophenol for amplification of chemiluminescence. A comparison of the chemiluminescence intensity with time has demonstrated a better stability for the substrate mixture prepared on borate buffer. A decrease in chemiluminescence signal with time was proportional to the decrease in background signal, thus rendering stability of the signal/background ratio for 3 to 30 minutes. Due to optimizing the substrate mixture composition and conditions of chemiluminescence recording, the reached detection limits for colorimetric and chemiluminescent ELISA’s were, respectively, 0.6 ng/ml and 0.08 ng/ml. The measurement range was extended by more than 20 times for chemiluminescent ELISA. The chemiluminescent ELISA for anti-erythropoietin antibody detection showed a 1.9 to 2.6-fold increase in sensitivity for rabbit serum, and 1.8 to 8.9-fold for guinea pigs serum. Good correlation of results was found for quantitative detection of antibodies in rabbit sera using the two methods (R = 0.981). Thus, chemiluminescent ELISA allowed develop a more sensitive detection technique of IgG antibodies to human erythropoietin.

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Slepov, E. V., R. A. Zukov, M. S. Serbaeva, A. M. Karapetyan, O. V. Kashaeva, A. Yu Pavlenko, and Yu V. Kozina. "Possibility of modification of peripheral blood neutrophils functional activity during chemoradiotherapy in patients with anorectal cancer." Cancer Urology 19, no. 1 (May 12, 2023): 133–40. http://dx.doi.org/10.17650/1726-9776-2023-19-1-133-140.

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Background. Ionizing radiation is an effective antitumor therapy, but it has a serious negative effect on the immune system requiring the use of radiation reaction prevention and reduction methods. Neutrophils are a sensitive element of the immune system both in interaction with tumor tissue and in response to radiation injury.Aim. To evaluate functional activity of peripheral blood neutrophils by chemiluminescent analysis in patients with anorectal cancer after radiotherapy.Materials and methods. The study included 80 patients with anorectal cancer. Patients received chemo- and radio-therapy with 3D conformal radiotherapy and radiation therapy under visual control, followed by the use of radioprotector and without it. Neutrophil activity determined by chemiluminescent analysis.Results. In patients with anorectal cancer found maximum spontaneous and induced chemiluminescence acceleration. The chemiluminescence activation index with luminol is lower in patients with anorectal cancer, and with lucigenin shows no differences with the control group. The low luminol chemiluminescence maximum intensity, as well as the decrease in the synthesis of reactive secondary oxygen species in enzymatic systems, is likely regulatory intracellular limitations consequence. After treatment, patients with radioprotector showed a decrease in the number of parameters with statistically significant differences with the control group. Undesirable phenomena associated with sodium deoxyribonucleate therapy not detected in anorectal cancer patients during radiotherapy and subsequent observation period.Conclusion. During treatment, differences in chemiluminescence parameters suggest that ionizing radiation affects them in patients with anorectal cancer receiving standard chemoradiotherapy. Use of chemoradiotherapy with a radioprotector leads to indirect restoration of cellular functional activity. This is confirmed by luminol-dependent chemiluminescence faster reaching its maximum and a decrease in the number of significant differences from the control group after the start of the drug treatment.

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Maity, Santanu, Xiaojian Wang, Subhamoy Das, Maomao He, Lee W. Riley, and Niren Murthy. "A cephalosporin–chemiluminescent conjugate increases beta-lactamase detection sensitivity by four orders of magnitude." Chemical Communications 56, no. 24 (2020): 3516–19. http://dx.doi.org/10.1039/c9cc09498a.

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A beta-lactamase chemiluminescent probe, termed CCP, which can for the first time detect beta-lactamase activity via chemiluminescence and 4-orders of magnitude higher than commercial fluorescent probe.

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Shelef, Omri, Adam C. Sedgwick, Sabina Pozzi, Ori Green, Ronit Satchi-Fainaro, Doron Shabat, and Jonathan L. Sessler. "Turn on chemiluminescence-based probes for monitoring tyrosinase activity in conjunction with biological thiols." Chemical Communications 57, no. 86 (2021): 11386–89. http://dx.doi.org/10.1039/d1cc05217a.

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We report a chemiluminescent probe that permits the paired detection of tyrosinase (Tyr) and biological thiols. The Tyr-formed benzoquinone intermediate reacts with GSH and produces an enhanced chemiluminescence response.

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Lin, Ke Li, Tong Yang, Fang Fang Zhang, Gang Lei, Hong Yan Zou, Yuan Fang Li, and Cheng Zhi Huang. "Luminol and gold nanoparticle-co-precipitated reduced graphene oxide hybrids with long-persistent chemiluminescence for cholesterol detection." Journal of Materials Chemistry B 5, no. 35 (2017): 7335–41. http://dx.doi.org/10.1039/c7tb01607g.

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Luminol and AuNP dual-functionalized rGO hybrids (rGO/AuNP/luminol) have been synthesized to generate long-persistent chemiluminescence, which can be used as a chemiluminescent biosensing platform for the detection of cholesterol.

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Roby, R. J., A. J. Hamer, E. L. Johnson, S. A. Tilstra, and T. J. Burt. "Improved Method for Flame Detection in Combustion Turbines." Journal of Engineering for Gas Turbines and Power 117, no. 2 (April 1, 1995): 332–40. http://dx.doi.org/10.1115/1.2814099.

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A fast response chemiluminescent flame detection approach is presented along with field test results from a fiber optic based flame detector device. Chemiluminescence, the light given off by molecules formed in their excited states, has long been recognized as a diagnostics method for use in combustion. The recent advent of higher quality optical fibers with improved transmission properties in the UV, as well as UV optical detectors, has made the use of chemiluminescence for gas turbine diagnostics and monitoring practical. Advances in combustor designs on new low-emissions machines as well as reliability issues with some existing machines are creating the need for improved flame dynamics measurements as well as improvements in reliability for existing measurements such as combustor flame detection. This paper discusses the technology, principle of operation, and detectors that operate on the chemiluminescence principle.

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Smirnova, O. V., and E. S. Ovcharenko. "Functional activity of the monocyte immune link in gastric adenocarcinoma." Medical Immunology (Russia) 25, no. 5 (June 1, 2023): 1117–22. http://dx.doi.org/10.15789/1563-0625-fao-2687.

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Stomach cancer is in the top ten in terms of prevalence and ranks 4th in terms of causes of death worldwide. The most common and most aggressive variant of gastric cancer is adenocarcinoma. The monocytic link of immunity provides the main line of the body's fight against malignant cells, while in patients with adenocarcinoma it is insufficiently studied. The purpose of the study was to evaluate the functional activity of monocytes in patients with gastric adenocarcinoma at different stages of the disease.Individuals (n = 164) were examined, among whom 85 were diagnosed with stage I-IV stomach adenocarcinoma. The study also involved 79 apparently healthy donors. The functional activity and oxygen-dependent phagocytosis of monocytes were assessed by the chemiluminescent method. Luminol was used as a chemiluminescence inducer. The respiratory burst was activated with opsonized zymosan.In patients with stomach adenocarcinoma, at rest (spontaneous chemiluminescence), an increase in the time the curve reached the maximum intensity of chemiluminescence (Tmax = 7957 s), the area under the chemiluminescence curve (Squr = 0.2 x 106), the activation index (1.89 c. u.) and a decrease in the maximum value of chemiluminescence intensity (Imax = 424 c. u.) relative to the control group (Tmax = 5533 s, Squr = 0.011 x 106, activation index = 0.88 c. u., Imax = 424 c. u., p < 0.05) were seen. When chemiluminescence is induced in patients with stomach adenocarcinoma, a statistically significant predominance of Squr is fixed (0.46 x 106, in the control group Squr = 0.031 x 106). Also, in the group of patients with stomach adenocarcinoma, monocytic phagocytosis was reduced by more than 2 times (29% vs 84% in the control group, p < 0.05). When analyzing the studied parameters, depending on the stage of the disease, it was found that the violation of the chemiluminescent reaction in patients with stomach adenocarcinoma is fixed already at an early stage. At the same time, in patients with stage IV stomach adenocarcinoma, the indicators of spontaneous and induced chemiluminescence are more than 2 times different from those in the control group and patients at stage I of the disease. The identified features indicate a decrease in the effectiveness of immune reactions of the monocytic link in stomach adenocarcinoma already in the early stages of the disease and can be used to detect early signs of immune disorders and optimize therapeutic approaches in this disease.

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Wang, Baoqu, Zhenzhou Chen, Xiaohong Cen, Yuqing Liang, Liyi Tan, En Liang, Lu Zheng, Yanjun Zheng, Zhikun Zhan, and Kui Cheng. "A highly selective and sensitive chemiluminescent probe for leucine aminopeptidase detection in vitro, in vivo and in human liver cancer tissue." Chemical Science 13, no. 8 (2022): 2324–30. http://dx.doi.org/10.1039/d1sc06528a.

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The first chemiluminescent probe for the detection of LAP is described. It shows a highly selective, sensitive and rapid chemiluminescence response for the detection of LAP in vitro and in vivo, and enables the differentiation of liver cancer.

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Gao, Jie, Zhengjun Chen, Xinmin Li, Mingyan Yang, Jiajia Lv, Hongyu Li, and Zeli Yuan. "Chemiluminescence in Combination with Organic Photosensitizers: Beyond the Light Penetration Depth Limit of Photodynamic Therapy." International Journal of Molecular Sciences 23, no. 20 (October 19, 2022): 12556. http://dx.doi.org/10.3390/ijms232012556.

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Photodynamic therapy (PDT) is a promising noninvasive medical technology that has been approved for the treatment of a variety of diseases, including bacterial and fungal infections, skin diseases, and several types of cancer. In recent decades, many photosensitizers have been developed and applied in PDT. However, PDT is still limited by light penetration depth, although many near-infrared photosensitizers have emerged. The chemiluminescence-mediated PDT (CL-PDT) system has recently received attention because it does not require an external light source to achieve targeted PDT. This review focuses on the rational design of organic CL-PDT systems. Specifically, PDT types, light wavelength, the chemiluminescence concept and principle, and the design of CL-PDT systems are introduced. Furthermore, chemiluminescent fraction examples, strategies for combining chemiluminescence with PDT, and current cellular and animal applications are highlighted. Finally, the current challenges and possible solutions to CL-PDT systems are discussed.

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Plekhanov A. Yu., Puzyk M. V., Usikov A. S., Roenkov A. D., Lebedev A. A., Lebedev S. P., Klotchenko S. A., Vasin A. V., and Makarov Yu. N. "Chemiluminescence of a Functionalized Graphene Surface." Optics and Spectroscopy 130, no. 9 (2022): 1141. http://dx.doi.org/10.21883/eos.2022.09.54835.3628-22.

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To analyze the modification of the functionalized surface of graphene by protein molecules, a chemiluminescent enzyme-linked immunosorbent assay based method was proposed. Using the example of functionalized graphene (FG) purification, the possibilities of chemiluminescent control of the state of its surface are shown. Methods for purifying FG from protein molecules with the restoration of the ability to resorb protein molecules are discussed. It has been shown that the FG surface can be cleaned from sorbed proteins, and a biosensor can be designed again based on such purified graphene, including with a different specificity. Therefore, the graphene sensor can be used repeatedly. Keywords: functionalized graphene, 1-pyrenemethylamine hydrochloride, cyclic voltammetry, chemiluminescence, monoclonal antibodies, biosensors

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Dudina, M. A., A. A. Savchenko, S. A. Dogadin, and I. I. Gvozdev. "The chemiluminescent and enzymatic activity of blood neutrophils in patients with Graves' disease depending on hyperthyroidism compensation." Clinical and experimental thyroidology 18, no. 1 (July 11, 2022): 4–14. http://dx.doi.org/10.14341/ket12717.

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BACKGROUND: Oxidative stress in Graves’ disease can potentiate the cytotoxicity of neutrophils and support autoimmune inflammation. Elimination of hyperthyroidism with conservative therapy with thiamazole only in some cases leads to remission, which determines the necessity to search an immunological markers for etiotropic therapeutic approaches in treatment of the disease.AIM: To study the chemiluminescent and enzymatic activity of peripheral blood neutrophils in patients with Graves’ disease depending on hyperthyroidism compensation to determine the intracellular targets of immunotropic treatment.MATERIALS AND METHODS: Spontaneous and zymosan-induced chemiluminescence was evaluated using a 36-channel chemiluminescence analyzer «BLM-3607» (MedBioTech, Krasnoyarsk). The reactivity of peripheral blood neutrophils was characterized by: Tmax — the rate of development of the chemiluminescent reaction, Imax — the maximum level of reactive oxygen species (ROS) synthesis and the area under the chemiluminescence curve (S — total synthesis of ROS for 90 minutes of measurement). The activity of NAD(P)-dependent dehydrogenases in neutrophils was determined using the bioluminescent method.RESULTS: The prospective study included 126 women with Graves’ disease, aged 18 to 65 years, 93 (73.81%) with compensated and 33 (26.19%) with uncompensated hyperthyroidism. In uncompensated hyperthyroidism, the indicator S of spontaneous ROS and zymosan-induced lucigenin-dependent chemiluminescence increases significantly, both relative to the control and to the values of compensated hyperthyroidism patients. Antigenic neutrophils stimulation during luminol-dependent chemiluminescence in patients with recurrent hyperthyroidism demonstrated more than tenfold increase in the total synthesis of ROS relative to the control, but no statistically significant differences with compensated hyperthyroidism patients. A high level of NADH-GDG was established in patients with recurrent hyperthyroidism, both relative to the control range and to the patients with euthyroidism.CONCLUSION: Violation of oxygen peripheral blood neutrophils metabolism in patients with euthyroidism mainly affects the production of ROS, which is associated with hyperthyroidism compensation and the immunosuppressive effect of thiamazole. In patients with recurrent hyperthyroidism, there are more changes in the production of high-energy oxidants not only at initial oxidative reactions stage, but also at the level of secondary ROS, indicating the activation of cellular response immunological mechanisms.

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Motoyoshiya, Jiro. "Chemiluminescence in Organic Reactions: Fundamental Investigation and Application of Peroxyoxalate Chemiluminescence and Related Chemiluminescent Reactions." Journal of Synthetic Organic Chemistry, Japan 70, no. 10 (2012): 1018–29. http://dx.doi.org/10.5059/yukigoseikyokaishi.70.1018.

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Miller, S. A., M. S. Morton, and A. Turkes. "Chemiluminescence Immunoassay for Progesterone in Plasma Incorporating Acridinium Ester Labelled Antigen." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 25, no. 1 (January 1988): 27–34. http://dx.doi.org/10.1177/000456328802500103.

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A sensitive, solid-phase chemiluminescence immunoassay suitable for determining progesterone concentrations in plasma has been developed. The solid-phase antiserum was prepared by coupling a monoclonal progesterone-antibody, raised against a progesterone-11α-hemisuccinyl/bovine serum albumin conjugate, to cyanogen bromide activated cellulose. An 11α-progesteryl-2-carboxymethyltyramine-4-(10-methyl)-acridinium-9-carboxylate conjugate was used as the chemiluminescent label. The assay had a lower limit of sensitivity of 3 pg/assay tube and satisfied accepted validation criteria. Progesterone concentrations determined by chemiluminescence assay were in good agreement not only with a radioimmunoassay in routine use but also with a gas chromatography-mass spectrometry procedure.

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Nie, Fei, and Jiuru Lu. "Novel chemiluminescence system with calcein as chemiluminescent reagent." Luminescence 22, no. 5 (2007): 480–86. http://dx.doi.org/10.1002/bio.988.

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Silva, José Pedro, Patricia González-Berdullas, Joaquim C. G. Esteves da Silva, and Luís Pinto da Silva. "Development of a Coelenterazine Derivative with Enhanced Superoxide Anion-Triggered Chemiluminescence in Aqueous Solution." Chemosensors 10, no. 5 (May 5, 2022): 174. http://dx.doi.org/10.3390/chemosensors10050174.

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Superoxide anion is a reactive oxygen species (ROS) of biological interest. More specifically, it plays a role in intra- and intercellular signaling, besides being associated with conditions such as inflammation and cancer. Given this, efforts have been made by the research community to devise new sensing strategies for this ROS species. Among them, the chemiluminescent reaction of marine Coelenterazine has been employed as a sensitive and dynamic probing approach. Nevertheless, chemiluminescent reactions are typically associated with lower emissions in aqueous solutions. Herein, here we report the synthesis of a new Coelenterazine derivative with the potential for superoxide anion sensing. Namely, this novel compound is capable of chemiluminescence in a dose-dependent manner when triggered by this ROS species. More importantly, the light-emission intensities provided by this derivative were relevantly enhanced (intensities 2.13 × 101 to 1.11 × 104 times higher) in aqueous solutions at different pH conditions when compared to native Coelenterazine. The half-life of the chemiluminescent signal is also greatly increased for the derivative. Thus, a new chemiluminescence molecule with significant potential for superoxide anion sensing was discovered and reported for the first time.

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Ikkes, L. A., A. A. Savchenko, G. P. Martinova, and I. I. Gvozdev. "Granulocyte-macrophage colony-stimulating factor: prospects for use in children with infectious mononucleosis." Voprosy praktičeskoj pediatrii 17, no. 5 (2022): 43–51. http://dx.doi.org/10.20953/1817-7646-2022-5-43-51.

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Objective. To analyze the production of active oxygen species (AOS) by peripheral blood neutrophils in children with infectious mononucleosis (IM) after their exposure to granulocyte-macrophage colony-stimulating factor (GM-CSF) in vitro. Patients and methods. We examined 78 children aged 3 to 11 years in the acute period of IM (including 47 children aged 3–6 years and 31 children aged 7–11 years). The control group comprised 40 healthy children matched for age. Luminoldependent chemiluminescence (CL) of peripheral blood neutrophils was assessed using the method of De Sole et al. The following CL parameters were measured: time to reach the maximum (Tmax), maximum value (Imax), and the area (S) under the chemiluminescent curve. Results. Children with IM (from both age groups) demonstrated lower spontaneous lucigenin-dependent chemiluminescence of neutrophils than controls: Tmax (p < 0.001 and p = 0.036, respectively), Imax (p = 0.024 and p = 0.025), and S (p = 0.01). Incubation of neutrophils with GM-CSF caused changes in luminol-dependent chemiluminescence only in children with MI from both age groups: Tmax decreased, whereas Imax and S increased. IM patients aged 7–11 years were found to have higher levels of chemiluminescence of neutrophils exposed to GM-CSF in vitro than IM patients aged 3–6 years (Tmax (p < 0.001) and SGM-CSF/Ssp. (p = 0.024)). Conclusion. Our findings suggest that GM-CSF can increase functional activity of neutrophils. This might facilitate adaptive immunity at periphery. Key words: infectious mononucleosis, neutrophilic granulocytes, chemiluminescence of neutrophils, granulocyte-macrophage colony stimulating factor

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Rahman, Habibur. "Analytical Applications of Permanganate as an Oxidant in the Determination of Pharmaceuticals Using Chemiluminescence and Spectrophotometry: A Review." Current Analytical Chemistry 16, no. 6 (August 13, 2020): 670–86. http://dx.doi.org/10.2174/1573411015666190617103833.

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Background: Potassium permanganate is a green and versatile industrial oxidizing agent. Due to its high oxidizing ability, it has received considerable attention and has been extensively used for many years for the synthesis, identification, and determination of inorganic and organic compounds. Objective: Potassium permanganate is one of the most applicable oxidants, which has been applied in a number of processes in several industries. Furthermore, it has been widely used in analytical pharmacy to develop analytical methods for pharmaceutically active compounds using chemiluminescence and spectrophotometric techniques. Results: This review covers the importance of potassium permanganate over other common oxidants used in pharmaceuticals and reported its extensive use and analytical applications using direct, indirect and kinetic spectrophotometric methods in different pharmaceutical formulations and biological samples. Chemiluminescent applications of potassium permanganate in the analyses of pharmaceuticals using flow and sequential injection techniques are also discussed. Conclusion: This review summarizes the extensive use of potassium permanganate as a chromogenic and chemiluminescent reagent in the analyses of pharmaceutically active compounds to develop spectrophotometric and chemiluminescence methods since 2000.

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Savchenko, Andrei A., Ruslan A. Zukov, Michael A. Firsov, Evgeniy V. Slepov, Vasiliy D. Belenyuk, I. I. Gvozdev, and A. G. Borisov. "Phagocytic and chemiluminescent activity of blood neutrophils in patients with bladder cancer." Russian Journal of Oncology 26, no. 2 (October 6, 2022): 39–48. http://dx.doi.org/10.17816/1028-9984-2021-26-2-39-48.

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BACKGROUND: Tumor microenvironment modulates (including with the help of metabolites) the functional activity of the neutrophils that contribute to the reprogramming of the antitumor activity into a protumor one. AIMS: To study the phagocytic and chemiluminescent activity of neutrophils in patients with bladder cancer (BC) under the influence of metabolites of the tumor microenvironment in vitro. MATERIALS AND METHODS: We examined 37 patients with superficial BC (T1,а,isN0M0) and considered 32 healthy individuals as a control group. Neutrophils isolated from their blood were incubated in vitro with lactate, ADP, and glutamate. Phagocytic activity was examined using flow cytometry, and the intensity of the respiratory burst of neutrophils was evaluated via chemiluminescent analysis. RESULTS: In patients with BC, the phagocytic index (PhI) values are reduced compared to the control sample (without in vitro metabolite exposure) and when exposed to glutamate, while the effect of lactate on cells causes an increase in the phagocytic number and PhI. Moreover, under the influence of lactate in vitro, the activity of spontaneous and zymosan-induced chemiluminescence of neutrophils decreases. ADP causes a decrease in spontaneous chemiluminescence only. Finally, under the influence of glutamate, the indicators of spontaneous and induced chemiluminescence decrease. CONCLUSIONS: Under the influence of lactate and ADP (products of tumor cells), the phagocytic activity of a population of immature neutrophils is stimulated, which leads to myeloid suppressor cells that inhibit antitumor immunity. Thus, metabolites of the tumor microenvironment modulate the activity of the respiratory burst of neutrophils in patients with BC.

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Usmani, Shaista S., Jerrold S. Schlessel, Concepcion G. Sia, Shahid Kamran, and Shahnaz D. Orner. "Polymorphonuclear Leukocyte Function in the Preterm Neonate: Effect of Chronologic Age." Pediatrics 87, no. 5 (May 1, 1991): 675–79. http://dx.doi.org/10.1542/peds.87.5.675.

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In this study, effect of chronologic age on polymorphonuclear leukocyte (PMN) chemiluminescence and random and chemotactic motility was evaluated in 38 stable preterm neonates of less than 32 weeks' gestation during the first month of life. Chemiluminescence and random and chemotactic motility of PMNs from preterm neonates were first evaluated at mean postnatal age of 9.8 days and then weekly for an ensuing 21-day period. For comparison, one blood sample was obtained for PMN functions from 14 healthy term neonates younger than 72 hours of age and seven normal adults. On day 1 PMN chemiluminescence and random and chemotactic motility values in preterm neonates were significantly lower (P < .001) compared with those in term neonates and PMN function values of term neonates were significantly lower (P < .001) than those of adults. Although initial PMN chemiluminescence and random and chemotactic motility values in preterm neonates were depressed, subsequent values on days 7, 14, and 21 increased significantly (P < .002). On day 21 (mean postnatal age of 30.8 days) no differences existed in chemiluminescent activity and random motility between preterm and term neonates; chemotactic motility in preterm neonates, however, remained impaired. Mean cumulative age (gestational age at birth plus postnatal age) of preterm neonates on day 21 of study was 32.5 weeks, suggesting that chronologic age has more effect on maturational changes in PMN functions than gestational age.

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FlØ, REINHARDT W., ARE NaeSs, GRETHE Albrektsen, and CLAUS OLA Solberg. "Isoprinosine stimulates granulocyte chemiluminescence and inhibits monocyte chemiluminescencein vitro." APMIS 102, no. 1-6 (January 1994): 249–54. http://dx.doi.org/10.1111/j.1699-0463.1994.tb04872.x.

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Kricka, Larry J. "Chemiluminescence." Cold Spring Harbor Protocols 2018, no. 4 (April 2018): pdb.top098236. http://dx.doi.org/10.1101/pdb.top098236.

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McCapra, Frank. "Chemiluminescence." Journal of Photochemistry and Photobiology A: Chemistry 52, no. 2 (May 1990): 345–46. http://dx.doi.org/10.1016/1010-6030(90)80013-n.

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Fethi, F., F. Poblete, E. Martinez, A. Gonzalez Urena, and G. Taieb. "Reaction Cross Sections of Ca (41S, 43P and 31D States) With Halogenated Compounds and Water." Laser Chemistry 16, no. 4 (January 1, 1996): 229–43. http://dx.doi.org/10.1155/1996/29359.

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By using two independent and different methods, absolute values of the reaction cross-sections have been determined for the following chemiluminescent reactions Ca(3P,1D)+Cl4C(CHBr3)→CaX*(A, B)(X=Cl, Br)+Cl3C(CHBr2) and Ca(1D)+H2O→CaOH*+H Both chemiluminescence and laser-induced fluorescence spectra are reported. A comparison with related types of reactions is also presented.

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Novikov, V., E. Yablokova, I. Shaev, N. Novikova, and E. Fesenko. "COMPARATIVE ANALYSIS OF THE INHIBITORY EFFECTS OF THE HYPOMAGNETIC FIELD AND COMBINED MAGNETIC FIELDS ON THE PRODUCTION OF REACTIVE OXYGEN SPECIES (ROS) BY NEUTROFILS." Russian Journal of Biological Physics and Chemisrty 8, no. 2 (May 21, 2024): 143–50. http://dx.doi.org/10.29039/rusjbpc.2023.0601.

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The article reveals that a decrease in the background production of reactive oxygen species (ROS) in the peritoneal neutrophils of mice after a short-term (40 minutes) stay in hypomagnetic conditions (residual field  10 nT) at physiological temperatures, detected by the method of lucigenin-dependent chemiluminescence, is not accompanied by a violation of chemiluminescent response to respiratory burst activators: formylated peptide N-formyl-Met-Leu-Phe (fMLF) and phorbol ester of phorbol-12-meristat-13-acetate (PMA). These results were obtained by activated chemiluminescence using lucigenin or luminol and various combinations of ROS production activators (PMA and/or fMLF). In contrast, the action of combined parallel constant (induction 60 μT) and alternating (amplitude range 60-180 nT, frequency 49.5 Hz) magnetic fields (CMF) leads to a decrease in the chemiluminescent response to these activators. These data indicate different sources of ROS that respond to certain modes of CMF and hypomagnetic field in neutrophils. The conducted research and the previously obtained results enable to exclude the systems that control the respiratory burst in neutrophils from the main targets and acceptors that respond to short-term deprivation of the magnetic field.

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Agatsuma, S., H. Sekino, T. Nagoshi, and H. Watanabe. "Indoxyl-beta-D-glucuronide, the primary emitter of low-level chemiluminescence in plasma of hemodialysis patients." Clinical Chemistry 40, no. 8 (August 1, 1994): 1580–86. http://dx.doi.org/10.1093/clinchem/40.8.1580.

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Abstract Characteristic light emission induced by the oxidation of hydroxyl radicals has been found in plasma of hemodialysis patients (Agatsuma et al., Clin Chem 1992;38:48-55). We purified a primary emitter, a chemiluminescent component peaking at 430 nm, by anion-exchange chromatography and reversed-phase HPLC. By using proton nuclear magnetic resonance and authentic indoxyl compounds, we determined the primary emitter to be indoxyl-beta-D-glucuronide. Absorption and fluorescence spectra of the purified sample coincided well with those of authentic indoxyl-beta-D-glucuronide, as did the peak in the chemiluminescence emission spectrum. Retention time of the purified sample on reversed-phase HPLC, measured by fluorescence, was also in accordance with that of indoxyl-beta-D-glucuronide. To our knowledge, this is the first identification of a primary emitter of low-level chemiluminescence from a biological source.

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Brichagina, Anastasiya S., Mariya I. Dolgikh, Larisa R. Kolesnikova, and Larisa V. Natyaganova. "Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress." Acta Biomedica Scientifica 4, no. 1 (April 4, 2019): 133–37. http://dx.doi.org/10.29413/abs.2019-4.1.20.

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Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

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Tode, B., G. Messeri, F. Bassi, M. Pazzagli, and M. Serio. "Chemiluminescence immunoassay for somatomedin C in serum." Clinical Chemistry 33, no. 11 (November 1, 1987): 1989–93. http://dx.doi.org/10.1093/clinchem/33.11.1989.

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Abstract To select the best tracer for use in a competitive immunoassay, we conjugated human somatomedin C (SmC) to various chemiluminescent compounds via two different synthetic pathways. Naphthylhydrazides and arylhydrazides, used as the labels, were incorporated via their imidate or their succinimide esters. Conjugating the carboxy terminal of (amino ethyl)ethyl-isoluminol to SmC via a succinimide linkage supplied the most sensitive detection limit and the most immunoreactive conjugate. We developed an immunoassay based on the use of this conjugate, and evaluated dextran-coated charcoal, second-antibody precipitation, and solid-phase immunoprecipitation for separating bound and free label. This chemiluminescent method has a detection limit of 16 pg per tube, and it is accurate and precise. Correlation studies with a conventional radioimmunoassay (x) for SmC gave the following regression equation: y = 0.66x + 3.76 (r = 0.953, n = 30); the slight discrepancies between the two methods are probably ascribable to the use of different antibodies. We thus propose this chemiluminescence immunoassay as an inexpensive and sensitive alternative to radioimmunoassay for measuring SmC in serum or in extracts of serum.

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Sławinska, Danuta, and Janusz Sławinski. "Chemiluminescence of cereal products. II. Chemiluminescence spectra." Journal of Bioluminescence and Chemiluminescence 13, no. 1 (January 1998): 13–19. http://dx.doi.org/10.1002/(sici)1099-1271(199801/02)13:1<13::aid-bio461>3.0.co;2-f.

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Li, Ruiji, Qi Fang, Bingzhao Mou, Minghui Chen, Qiwen Tang, Baojun Shen, Dong Wang, and Wei Li. "A convenient one pot synthesis, crystal structure and chemiluminescence of S,S-diphenyl ethanebis(thioate) compound." Bulletin of the Chemical Society of Ethiopia 37, no. 5 (June 30, 2023): 1185–91. http://dx.doi.org/10.4314/bcse.v37i5.11.

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ABSTRACT. A compound S,S-bis(2-(prop-1-yn-1-yl)phenyl) ethanebis(thioate) is synthesized through one-pot synthesis. The lithiation reaction of 3-bromo-2-methylbenzo[b]thiophene by using n-butyllithium, is followed by conversion to the corresponding organocopper compound, and subsequent reaction with oxalyl chloride to afford the target compound. The chemical structure of the final compound is well confirmed by NMR, HRMS and X-ray analysis. Meanwhile, the proposed reaction mechanism is fully discussed. Furthermore, the chemiluminescent reaction by using target compound to release light is also investigated. This work provides a convenient synthetic approach for obtaining S,S-diphenyl ethanebis(thioate) compound and broadening its application in chemiluminescence. KEY WORDS: One pot synthesis, S,S-Diphenyl ethanebis(thioate) compound, Crystal structure, Reaction mechanism, Chemiluminescence Bull. Chem. Soc. Ethiop. 2023, 37(5), 1185-1191. DOI: https://dx.doi.org/10.4314/bcse.v37i5.11

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Musiani, Monica, Marialuisa Zerbini, Simona Venturoli, Giovanna Gentilomi, Giorgio Gallinella, Elisabetta Manaresi, Michelangelo La Placa, Antonietta D'Antuono, Aldo Roda, and Patrizia Pasini. "Sensitive Chemiluminescence In Situ Hybridization for the Detection of Human Papillomavirus Genomes in Biopsy Specimens." Journal of Histochemistry & Cytochemistry 45, no. 5 (May 1997): 729–35. http://dx.doi.org/10.1177/002215549704500511.

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We developed a sensitive chemiluminescence in situ hybridization assay for detection of human papillomavirus (HPV) DNA for objective and semiquantitative evaluation of the results. The hybridization reaction was performed using either digoxigenin-, biotin-, or fluorescein-labeled probes, visualized with alkaline phosphatase as the revealing enzyme and a highly sensitive 1,2 dioxetane phosphate as chemiluminescent substrate. The light emitted from the hybridized probes was detected, analyzed, and measured using a high-performance, low light-level imaging luminograph connected to an optical microscope and to a personal computer for quantification of the photon fluxes and for image analysis. The system operated in consecutive steps: First, hybridized specimens were recorded in transmitted light. Then the net luminescent signal was recorded, and then an overlay of the two images provided by the transmitted light and by the luminescent signal allowed the spatial distribution of the target DNA to be localized, measured, and evaluated. Biopsy specimens from different pathological conditions associated with HPV, which had previously been proved positive for HPV DNA with the polymerase chain reaction (PCR), were analysed. The chemiluminescence in situ hybridization proved sensitive and specific with digoxigenin-, biotin-, or fluorescein-labeled probes, and provided an objective evaluation of the results. The results obtained with chemiluminescence in situ hybridization were also compared with results obtained with in situ hybridization with colorimetric detection, with good concordance of the data. Chemiluminescence in situ hybridization therefore offers the possibility of detecting HPV DNA with great sensitivity in biopsy specimens. Moreover, the images of the samples, stored in the computer, are a permanent record of the reaction and can also be sent for evaluation or comparison to other laboratories using computer networks.

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Luo, Liezhao, Ting Li, Jiangge Deng, Runzhou Zhao, Jinkui Wang, and Lijun Xu. "Experimental Investigation on Self-Excited Thermoacoustic Instability in a Rijke Tube." Applied Sciences 12, no. 16 (August 11, 2022): 8046. http://dx.doi.org/10.3390/app12168046.

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The experimental investigations into the thermoacoustic instability in a Rijke tube are presented. In order to capture the dynamics of the temperature, a single-ended tunable diode laser absorption spectroscopy (TDLAS) technique was developed, with a measurement rate of 5 kHz. The temperature was found to fluctuate periodically at a dominant frequency of 230 Hz, corresponding to the fundamental frequency of the Rijke tube used in the experiment. The flame chemiluminescence was detected by a high-speed camera to demonstrate flame response to thermoacoustic instability. It was evident that the flame front stretched regularly and had jagged edges. To quantitate the fluctuations of chemiluminescence intensity, the relative area was defined. According to the result, the intensity also oscillated at 230 Hz. Furthermore, the same feature was found in regard to pressure at the exit of the Rijke tube. Compared with temperature and chemiluminescent intensity, the pressure oscillations presented the most approximate standard waveform, as they suffered the least disruptions. The results indicated that the dominant frequencies of temperature, chemiluminescent intensity and pressure were consistent, in accordance with the fundamental frequency of the Rijke tube in the experiment. In addition, etalon effects on the TDLAS signals were mitigated efficiently by a lowpass filter.

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Jiang, Qingqing, Fei Nie, and Jiuru Lu. "Chemiluminescence determination of bromhexine hydrochloride with morin as chemiluminescent reagent." Luminescence 23, no. 1 (January 2008): 32–36. http://dx.doi.org/10.1002/bio.1013.

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Capuano, Giuseppe E., Domenico Corso, Roberta Farina, Gianni Pezzotti Escobar, Giuseppe A. Screpis, Maria Anna Coniglio, and Sebania Libertino. "Miniaturizable Chemiluminescence System for ATP Detection in Water." Sensors 24, no. 12 (June 17, 2024): 3921. http://dx.doi.org/10.3390/s24123921.

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We present the design, fabrication, and testing of a low-cost, miniaturized detection system that utilizes chemiluminescence to measure the presence of adenosine triphosphate (ATP), the energy unit in biological systems, in water samples. The ATP–luciferin chemiluminescent solution was faced to a silicon photomultiplier (SiPM) for highly sensitive real-time detection. This system can detect ATP concentrations as low as 0.2 nM, with a sensitivity of 79.5 A/M. Additionally, it offers rapid response times and can measure the characteristic time required for reactant diffusion and mixing within the reaction volume, determined to be 0.3 ± 0.1 s. This corresponds to a diffusion velocity of approximately 44 ± 14 mm2/s.

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Yappert, M. Cecilia, and J. D. Ingle. "Absorption-Corrected Spectral Studies of the Lucigenin Chemiluminescence Reaction." Applied Spectroscopy 43, no. 5 (July 1989): 767–71. http://dx.doi.org/10.1366/0003702894202111.

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With a spectrometer based on two multichannel detectors, luminescence and absorption spectra are acquired simultaneously during the reaction between lucigenin and H2O2 in a basic solution. Alterations in the fluorescence and chemiluminescence spectral contours occur during the reaction, due to time-dependent inner filter effects caused by the changing absorption of the emission radiation by reactants, intermediates, or products. The spectrometer uses the measured absorbances and appropriate equations for automatic correction of luminescence spectra for inner filter effects. The corrected spectra demonstrate that the primary fluorescent product of the reaction, N-methyl acridone, is not the primary chemiluminescent emitting species.

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Stauff, Joachim, and Gerhard Stärk. "Chemilumineszenz von Photoprodukten polyzyklischer aromatischer Kohlenwasserstoffe und deren Carbonylverbindungen / Chemiluminescence of Photoproducts of Polycyclic Aromatic Hydrocarbons and their Carbonyl Compounds." Zeitschrift für Naturforschung B 41, no. 1 (January 1, 1986): 113–21. http://dx.doi.org/10.1515/znb-1986-0124.

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During photooxidation of polycyclic aromatic hydrocarbons (PAH) products can be formed which develop chemiluminescence on treatment with bases. Flash photolysis experiments show that this is the case only after previous formation of cation radicals, e.g. in the presence of CCl4 as solvent or of e-acceptors in aprotic solvents. These radicals react with oxygen to peroxy-radicals which can combine to several kinds of peroxides. Primary and secondary peroxides are the sources of chemiluminescent activity.Chemiluminescent peroxides can also be obtained by irradiation of PA H carbonyl com pounds in protic solvents under nitrogen. It is assumed that two excited CO groups combine exceptionally with their O-atom s thus creating a peroxide bond. 24 aromatic aldehydes, ketones, dicarboxylic acid anhydrides and coumarines develop chemiluminescence after illumination with wavelengths ≥ 320 nm with intensities varying 4 magnitudes of order.The sensitivity of the photochemiluminescent method is sufficient to detect amounts of PA H and their CO derivatives in the ppb to ppm range.

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Journal articles on the topic 'Chemiluminescence'

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