Dissertations / Theses on the topic 'Cervical artificial insemination (AI)'
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Januskauskas, Aloyzas. "Assessment of viability and function of post-thaw spermatozoa from Swedish dairy AI bulls /." Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 1999. http://epsilon.slu.se/avh/1999/91-576-5435-2.pdf.
Full textDorsey, Benjamin Reese. "Effect of Timing of Insemination and Synchronization of Estrus Method on Artificial Insemination (AI) Pregnancy Rates in Beef Heifers." Thesis, Virginia Tech, 2005. http://hdl.handle.net/10919/42873.
Full textMaster of Science
Sbardella, Pedro Ernesto. "Inseminação artificial pós-cervical em primíparas suínas." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/71572.
Full textThe study evaluated the reproductive performance of primiparous sows submitted to post cervical insemination (PCAI) compared to cervical artificial insemination (CAI). Difficulty with catheter introduction, occurrence of bleeding or semen backflow during insemination, and volume and sperm cell backflow up to 60 min after insemination were also evaluated. Sows were homogenously distributed, according to body weight loss in lactation, lactation length, weaned piglets, weaning-to-estrus interval and total born in previous farrowing, in two treatments: PCAI (n= 165) with 1.5 x 109 sperm cells in 45 ml and CAI (n= 165) with 3 x 109 sperm cells in 90 ml. Transabdominal real time ultrasonography was performed at the moment of standing heat and 24 h after last insemination. There was no difference between treatments in farrowing rate and litter size (P > 0.05). Successful passage of the intrauterine catheter in all the inseminations was possible in 86.8% (165/190) of sows initially allocated to PCAI treatment. Difficulty of introducing the catheter in at least one insemination did not affect the reproductive performance of PCAI sows (P > 0.05). Bleeding during insemination did not affect (P > 0.05) the farrowing rate in both treatments, but litter size was reduced in CAI and PCAI sows (P ≤ 0.06). Percentage of spermatozoa present in backflow was greater in CAI than PCAI treatment (P < 0.01). No difference (P > 0.05) was observed in farrowing rate and litter size according to the percentage of spermatozoa in backflow (Low: ≤ 20%; High: > 20%). It’s possible to perform the PCAI in primiparous sows with doses containing 1,5 x 109 sperm cells without detrimental on reproductive performance.
Ternus, Eduardo Miotto. "Performance reprodutiva de leitoas submetidas à inseminação artificial pós-cervical." Universidade do Estado de Santa Catarina, 2016. http://tede.udesc.br/handle/handle/2544.
Full textMade available in DSpace on 2018-03-15T12:45:06Z (GMT). No. of bitstreams: 1 PGCA16MA206.pdf: 750013 bytes, checksum: 70bddcd61c31dd2ee20002e2905f599e (MD5) Previous issue date: 2016-04-06
The objective of this study was to evaluate the reproductive performance of gilts subjected to post-cervical artificial insemination (PCAI) compared to traditional artificial insemination (TAI). We also evaluated the degree of difficulty in bypassing the cervix, time required to perform the insemination, presence of bleeding after insemination, semen backflow, as well as the volume and the total reflow cells 30 minutes after insemination. Gilts submitted to PCAI (n = 279) were inseminated with 45 mL doses of 1.5 x 10⁹ sperm cells and the ones submitted to TAI (n = 273) were inseminated with 80 mL doses with 2.5 x 10⁹ cells. The bypassing of the cervix was possible in 91.04% (254/279) of gilts. The difficulty bypassing the cervix in at least one of the gilt’s PCAI procedures happened with 41.58% (116/279) of the females, but it did not affect reproductive performance (P>0.05). The presence of bleeding after insemination did not affect the farrowing rate and total number of piglets born for both treatments (P>0.05). The average time needed to carry out the PCAI was 1.47 minutes and the TAI was 4.04 minutes. The percentage of sperm present in the reflux was higher in TAI than the PCAI, but no correlation was found between litter size and the percentage of sperm in reflux (P>0.05). There was no statistical difference (P>0.05) in farrowing rate (89.38% and 91.76%) and the total number of piglets born (11.63 and 11.81) between TAI and PCAI treatments, respectively. Thus, it is possible to perform the post-cervical artificial insemination in gilts without causing a reduction in reproductive performance, using doses with a concentration of 1.5 x 10⁹ sperm cells
O objetivo deste trabalho foi avaliar o desempenho reprodutivo de nulíparas submetidas à inseminação artificial pós-cervical (IAPC) comparada à inseminação artificial tradicional (IAT). Foram avaliados a ocorrência de sangramento, refluxo durante à inseminação, dificuldade no transpasse da cérvix, volume e o total de células refluídas até 30 minutos após a inseminação. As fêmeas submetidas à IAPC (n=279) foram inseminadas com doses na concentração de 1,5 x 109 diluídos em 45 mL e as fêmeas submetidas à IAT (n=273) foram inseminadas com doses na concentração de 2,5 x 109 diluídos em 80 mL. O transpasse da cérvix foi possível em 91,04% (254/279) das leitoas. A dificuldade no transpasse da cérvix, em pelo menos uma das inseminações, foi de 41,58% (116/279) e não comprometeu o desempenho reprodutivo (P>0,05). A presença de sangramento durante a inseminação não afetou a taxa de parto nem o número de leitões nascidos totais para ambos os tratamentos (P>0,05). O tempo médio necessário para a realização da IAPC foi de 1,47 minutos e a IAT foi de 4,04 minutos. O percentual de espermatozoides presentes no refluxo foi maior na IAT do que na IAPC, não sendo observada diferença no tamanho de leitegada de acordo com o percentual de espermatozoides no refluxo (P>0,05). Não houve diferença estatística (P>0,05) taxa de parto (89,38% e 91,76%) e no número de leitões nascidos totais (11,63 e 11,81) entre os tratamentos IAT e IAPC, respectivamente. Desta forma, podemos concluir que pode - se realizar a inseminação artificial pós-cervical em leitoas sem causar redução no desempenho reprodutivo, utilizando doses com concentração de 1,5 x 109 células espermáticas
Sena, Ana Luísa Gonçalves de. "Condução da reprodução em suínos : análise zootécnica e estudo comparativo de técnicas de inseminação artificial." Master's thesis, Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária. Instituto Superior de Agronomia, 2012. http://hdl.handle.net/10400.5/4077.
Full textNeste trabalho, pretendeu-se expor e caracterizar as performances reprodutivas e produtivas do efectivo de porcas de uma suinicultura industrial em ciclo fechado. Realizou-se igualmente um ensaio experimental comparativo de métodos de inseminação artificial. Neste ensaio foram avaliadas as diferenças no que diz respeito aos valores de fertilidade e prolificidade das porcas, cobertas por inseminação artificial cervical, inseminação pós-cervical com cateter de esponja e a inseminação artificial pós-cervical com cateter em espiral (kubus). Para tal utilizou-se um total de 123 porcas, distribuídas por 41 grupos de 3 porcas que foram inseminadas por um dos três métodos de inseminação em estudo. Na análise zootécnica das performances produtivas e reprodutivas das porcas, verificou-se que a época do ano (Verão, Outono, Inverno e Primavera) de cobrição das porcas não afectou os parâmetros reprodutivos e produtivos das porcas. Após a 3ª parição, verificou-se uma melhoria acentuada do intervalo entre partos, no intervalo desmame cobrição fecundante e na produtividade. No triénio 2008-2010 observou-se uma redução do intervalo desmame cobrição fecundante e do intervalo entre partos e do número de leitões por ninhada mas, a produtividade das porcas aumentou em cerca de 2 leitões/porca/ano. Os resultados do ensaio experimental mostraram que as diferentes técnicas de inseminação artificial das porcas não afectaram significativamente a sua fertilidade nem o número de leitões nascidos por ninhada.
ABSTRACT - Conduct of reproduction in pigs: zootechnical analysis and comparative study of artificial insemination techniques - The productive and reproductive performances of the sows in a farrowing to finish swine unit were characterised. Moreover an experimental study to compare three different methods of artificial insemination (AI) was performed. In this trial the fertility rate and the litter size of 123 sows (41 groups of 3 sows), fertilised by cervical AI or two post-cervical AI techniques (sponge or kubus catheter) were analysed. Results show that the matting season (summer, fall, winter, spring) have no significant effect on the productive and reproductive performances of the sows. After the 3rd farrowing, there was a significant decrease in the farrowing interval and in the weaning-conception period and an improvement in the number of piglets weaned per sow per year. During the years 2008-2010, despite a decrease in the number of piglets per litter, the productivity of the sows increased by 2 piglets/sow/year as a result of a decrease in the duration of the productivity cycle of the sows. Results of the experimental trial show that the different AI techniques have no significant effect in the fertility rate and in the number of piglets born per litter.
Fontana, Diogo Luiz. "Inseminação artificial pós-cervical em tempo fixo em porcas recebendo pLH no início do estro." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/72057.
Full textFixed-time artificial insemination (FTAI) associated to post cervical artificial insemination (PCAI) allows a wider use of high indexed boars and an expressive reduction on labor requirements in swine production. The aim of this study was to evaluate FTAI efficiency according to different AI protocols, using pLH – porcine luteinizing hormone - as ovulation inductor. A total of 597 weaned sows whose estrus detection was performed once daily (08:00 am) were allocated into three treatments: Control (n= 199) – the first insemination was performed at estrus onset (0 h) and repeated every 24 h thereafter, during estrus; FTAI1 (n= 199) - sows received a 5 mg (4 ml) i.m. injection of pLH at estrus onset, and were inseminated 24 h after, and FTAI2 (n= 199) - sows received 5 mg of pLH but were inseminated at estrus onset (0 h) and 24 h after. PCAI with homospermic doses (1.5 x 109 total sperm cells/50 ml) were performed in all treatments. Hormonal treatment did not affect the interval onset of estrus to ovulation (P>0.05). The number of inseminations was 2.9, 1.0 and 2.0 for Control, FTAI1 and FTAI2 respectively. Treatments did not affect farrowing rate and total born (P>0.05). Total piglets born per insemination dose was different (P<0.0001) among treatments (4.5, 12.5 and 6.2 for Control, FTAI1 and FTAI2 respectively). The use of pLH at estrus onset associated to a single fixed-time PCAI 24 h after does not compromise the reproductive performance of multiparous sows.
Sayre, Brian L. "Oxytocin-induced cervical dilation in sheep: mechanism of action and potential use for nonsurgical artificial insemination." Diss., Virginia Tech, 1995. http://hdl.handle.net/10919/40251.
Full textPerry, Kim Laura. "Role of hyaluronan in cervical relaxation of the ewe." Thesis, Royal Veterinary College (University of London), 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.558980.
Full textTusell, Palomero Llibertat. "Exploring the genetics of the efficiency of fertile AI dose production in rabbits." Doctoral thesis, Universitat Politècnica de València, 2011. http://hdl.handle.net/10251/11842.
Full textTusell Palomero, L. (2011). Exploring the genetics of the efficiency of fertile AI dose production in rabbits [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/11842
Palancia
Mellieon, Harold Irvin Jr. "Evaluation of the 5-day vs. 7-day Co-Synch + CIDR protocol in dairy heifers using timed AI." Thesis, Kansas State University, 2011. http://hdl.handle.net/2097/10749.
Full textDepartment of Animal Sciences and Industry
Jeffrey S. Stevenson
Our objectives were to determine: the effectiveness of upfront PGF2α injection to regress the corpus luteum; ovulation response to GnRH; and pregnancy outcomes. Dairy heifers (n = 545) from three locations (Florida, Kansas, and Mississippi) were assigned randomly to each of two treatments: 1) 25 mg of PGF2α injection and insertion of previously used autoclaved CIDR on d 7 followed by 100 µg of GnRH administered on d 5, and a 25 mg PGF2α injection at CIDR removal (7D) on d 0; 2) 100 µg of GnRH and insertion of previously used autoclaved CIDR on d 5 and 25 mg of PGF2α injection at CIDR removal (5D) on d 0. Artificial insemination (AI) occurred after detected estrus from d 0 to 3. Those heifers not detected in estrus were inseminated on d 3 and given a second 100 µg of GnRH. Blood collected on d 7 and 5 was assayed to determine concentrations of progesterone, presence of a CL (progesterone ≥1 ng/mL) on d 7, and whether luteolysis occurred in 7D heifers. Blood progesterone concentration from d 0 and 3 determined if luteolysis occurred in all heifers. Ovarian structure maps on d 5 and 0 were used to determine ovulation in response to GnRH on d 5. Pregnancy was determined on d 32 and 60 and intervening pregnancy loss was calculated. Of those heifers in the 7D treatment having progesterone ≥1 ng/mL on d 7, the proportion having progesterone <1 ng/mL 2 d later (luteolysis) was greater (P < 0.05) than that in the 5D treatment (43.0 vs. 22.9%, respectively). Total proportion of follicles that ovulated per heifer was numerically greater in the 7D treatment but only differed (P < 0.05) between locations. A treatment x location interaction was detected for pregnancy rates per AI. The Kansas location had no detectable treatment differences. In contrast, the 7D treatment produced greater (P < 0.05) pregnancy rates in the first replicate of the Florida location and at the Mississippi location. We concluded that the 5D protocol was not effective in producing acceptable luteolysis, pregnancy, and ovulation rates in comparison with the modified 7D protocol.
Fitzgerald, Robert. "A Comparison of Intrauterine and Cervical Artificial Insemination Rods on Farrowing Rate and Litter Size in Artificially Mated Sows." TopSCHOLAR®, 2005. http://digitalcommons.wku.edu/theses/496.
Full textPitt, Colin John. "Lowering Sperm Dose Rates in Frozen Semen for Bovine Artificial Breeding." The University of Waikato, 2007. http://hdl.handle.net/10289/2232.
Full textWilson, Dallas James Patterson David J. "Comparison of controlled internal drug release (CIDR)-based protocols to synchronize estrus and facilitate artificial insemination (AI) in postpartum beef cows." Diss., Columbia, Mo. : University of Missouri--Columbia, 2008. http://hdl.handle.net/10355/5687.
Full textSerret, Carolina Gonçalves. "Eficiência reprodutiva com inseminação artificial intra-cervical e pós-cervical, em fêmeas suínas, associada à concentração espermática e perfil estral." Universidade Federal de Pelotas, 2005. http://guaiaca.ufpel.edu.br/handle/123456789/1243.
Full textArtificial insemination (AI) is a technique intensively used in the swine farms nowadays, due to its benefits related to genetic improvement and health status, allowing the industry to market high-quality healthy products. The post-cervical AI (PCAI) allows the deposition of semen in the uterine body, using 1 x 10 9 spermatozoa dose. Thus, with PCAI the fertility of one particular boar can influence the reproductive performance of a much higher number of females than in ICAI-based systems. The objective of this study was to compare the subsequent reproductive performance of swine breeding females submitted to either intra-cervical artificial insemination (ICAI) with sperm concentrations of 3.5 x 10 9 spermatozoa per dose or post-cervical artificial insemination (PCAI) with 2, 1, or 0.5 x 10 9 spermatozoa per dose. The study was conducted in a commercial farm including 338 F1 females. Among them, 232 were first inseminated 12 h after estrus detection, receiving the subsequent doses after 12 h intervals, whereas 106 were inseminated right after ovulation diagnosis through real-time ultrasound. Conception and farrowing (FR) rates for ICAI (98.9% e 97.9%, respectively) did not differ (P < 0,001) from those observed for PCAI (87.1% e 84.3%, respectively). Total litter size did not differ across treatments (P = 0.09). Although TP did not differ (P = 0,40) for different parities, total litter size for primiparous females (9.1 ± 0.4) was lower (P = 0,0004) than for parity-2 and -3 females (11.5 ± 0.5 e 11.5 ± 0.2, respectively). There was a significant effect of an interaction between treatment and parity on total litter size (P < 0.05), generally characterized by the occurrence of lower litter sizes for PCAI in primiparous females than for other treatment by parity combinations. In conclusion, reproductive performance for PCAI and ICAI were similar with sperm concentrations up to 1 x 10 9 spermatozoa per dose.
A inseminação artificial (IA) é uma biotécnica reprodutiva que vem sendo intensamente utilizada na suinocultura, em função dos benefícios genéticos e sanitários, permitindo que as agroindústrias coloquem no mercado produtos saudáveis e de qualidade. A técnica de IA pós-cervical (IAPC) possibilita a deposição do sêmen no corpo do útero, utilizando doses inseminantes com de 1 x 10 9 espermatozóides/dose. Portanto, com a IAPC, a fertilidade do reprodutor utilizado como doador de sêmen, passa a influenciar o desempenho reprodutivo de um número três vezes maior de fêmeas, quando comparado a IA convencional. Este estudo teve como objetivo comparar o desempenho reprodutivo de fêmeas suínas submetidas a IAIC, com dose inseminante convencional com a IAPC com 3 diferentes concentrações (2; 1; e 0,5 x 10 9 espermatozóides/ dose). O estudo incluiu 338 fêmeas F1, sendo que, 232 fêmeas receberam a primeira dose inseminante 12 h após a detecção de cio e as doses subseqüentes em intervalos de 12 h, enquanto 106 fêmeas foram inseminadas imediatamente após a ovulação, confirmada por ultra-sonografia em tempo real. Taxas de concepção e parição (TPAR) para a IAIC (98,9% e 97,9%, respectivamente), foram maiores (P < 0,001) às observadas para a IAPC (87,1% e 84,3%, respectivamente). Tanto para taxa de concepção como TPAR, os índices observados para o T8 foram numericamente inferiores aos demais tratamentos, porém sem diferença significativa ( P > 0,05). O total de leitões nascidos por parto não diferiu entre os tratamentos (P = 0,09). Para as fêmeas com 1, 2 , 3 ou mais partos, a TPAR foi igual a 90,2%, 82,7% e 88,9% (P = 0,40). No entanto, o total de leitões nascidos foi influenciado pela ordem de parto (OP) das matrizes, sendo inferior (P = 0,0004) para fêmeas primíparas (9,1 ± 0,4) do que para fêmeas com 2 e 3 ou mais partos (11,5 ± 0,5 e 11,5 ± 0,2, respectivamente). Também houve interação entre o efeito dos tratamentos e a OP com relação ao tamanho de leitegada, pois a resposta foi inferior (P < 0,05) em fêmeas primíparas em alguns dos tratamentos com IAPC. Com isso, podemos concluir que o método de IAPC, em condições de rotina em uma granja comercial, atingiu índices de desempenho reprodutivo semelhantes quando comparado com o IAIC, com doses de até 1 x 10 9 espermatozóides.
Zuluaga, Velez Juan Federico. "Pre- and post-synchronization methodologies to enhance the efficiency of fixed timed artificial insemination in pharmacologically-controlled breeding systems with Bos indicus-influenced cattle." Thesis, Texas A&M University, 2006. http://hdl.handle.net/1969.1/4812.
Full textCasali, Renata. "Estratégias para viabilizar o uso de sêmen congelado na inseminação artificial cervical de ovinos." Universidade do Estado de Santa Catarina, 2014. http://tede.udesc.br/handle/handle/907.
Full textCoordenação de Aperfeiçoamento de Pessoal de Nível Superior
Oxidative stress and premature sperm capacitation, generated during cryopreservation of ram semen, reduces their viability, especially after cervical insemination. The use of seminal plasma (SP) and negative pressure have produced protection and the reversion of such damages. Two experiments evaluated these potential enhancers of cryotolerance, and a third experiment compared 2 methods of cervical AI. In experiment 1 ram semen was subjected to the treatments: (TC) control or negative pressure of 200mBar (P200); 500mBar (P500) and 800mBar (P800). In experiment 2, the PS from rams, stallions and bulls was lyophilized (L) and its protein measured. From each SP 600μg of protein per mL was aded to the freezing diluent used, compounding the experimental groups: control (TC), ovine PS (PSLO), bovine PS (PSLB) and equine PS (PSLE). Experiment 3 evaluated 2 methods of AI, the superficial cervical AI (G1), and deep intrauterine or cervical AI with clamping the vaginal fornix (G2). The in vitro data were subjected to ANOVA and test T, and the pregnancy rate to the chi square test, all with 5% significance level. In the experiment 1 higher progressive motility (PM) was observed in TC (49%) compared to P200 (40.9%), P500 (38.9%) and P800 (38.9%) treatments. In PM during the test the thermal resistance (TTR), MP after percoll (PP), acrosome integrity (IAC), IAPP and membrane integrity (MI), there was no difference between the groups. In cleavage rate P800 (34.5%) was less than P200 (51.2%) and P500 (50.9%) did not differ from the control (44.3%). In conclusion the P500 is the most appropriate for use in ram semen cryopreservation, enabling high rates of cleavage after heterologous IVF, maintain membrane integrity. Experiment 2 evaluated MP, MPPP and cleavage rate after heterologous IVF in all groups, with the best group compared with the control in: CASA system; acrossoma integrity (FITC-PSA), membrane stability (M540), chromatin integrity (acridine orange), apoptosis (annexin) and potential of mitochondria (Mitotracker). Also the PSLE showed higher cleavage rate (71.37%), indicating a greater ability to oocyte penetration. The PSLE showed higher VCL (PC-163.5μm/s, PSLE-186.2μm/s) and ALH (PC9μm PSLE-8.2μm) in CASA evaluation, compared to control. In flow cytometry the annexin test revealed a greater amount of non-apoptotic viable cells in PSLE (38.9%), compared to TC (32.1%). In experiment 3 there was no difference in pregnancy rates after superficial (33.3%) or deep and intrauterine (52.2%) IA, possibly due to the reduced number of animals used
O estresse oxidativo e a precoce capacitação espermática, gerados na criopreservação do sêmen ovino, reduzem sua viabilidade, principalmente na inseminação cervical. O uso de plasma seminal (PS) e a pressão negativa têm produzido a proteção e reversão desses danos. Dois experimentos avaliaram esses potenciais melhoradores da criotolerância, e um terceiro avaliou dois métodos de IA cervical. No experimento 1 o sêmen ovino foi submetido aos tratamentos: controle (TC), pressão de 200mBar (P200); 500mBar (P500) e 800mBar (P800). No experimento 2 o PS de carneiros, garanhões e touros foi liofilizado (L) e sua proteína dosada. De cada PS, o equivalente a 600μg de proteína por mL, foi adicionado ao diluente de congelamento, compondo os grupos experimentais: controle (TC), PS ovino (PSLO), PS bovino (PSLB) e PS equino (PSLE). O experimento 3 avaliou 2 métodos de IA, a cervical superficial (G1) e a cervical profunda com pinçamento do fundo de saco vaginal (G2). Os dados in vitro foram submetidos a análise de variância e teste T, e a taxa de prenhez ao chi-quadrado, todos com significância de 5%. No experimento 1, maior motilidade progressiva (MP) foi observada no TC (49%) frente aos tratamentos P200 (40,9%), P500 (38,9%) e P800 (38,9%). Na MP durante o teste de termo resistência (TTR), MP após percoll (PP), integridade de acrossoma (IAC), IACPP, integridade de membrana (IM) e IMPP, não houve diferença entre os grupos. Na clivagem P800 (34,5%) foi inferior a P200 (51,2%) e P500 (50,9%), não diferindo do controle (44,3%). Conclui-se que a P500 é a mais adequada para uso com sêmen ovino, não reduzindo a viabilidade após o congelamento e proporcionando elevada taxa de clivagem após FIV heteróloga. O experimento 2 avaliou MP, MPPP e clivagem após FIV heteróloga de todos os grupos, sendo o melhor grupo comparado ao controle através de: sistema CASA, integridade de acrossoma (FITC-PSA), estabilidade de membrana (M540); integridade de cromatina (acridina orange); apoptose (anexina) e potencial de mitocôndria (mitotracker). O PSLE apresentou a maior taxa de clivagem (71,37%), evidenciando sua maior capacidade de penetração nos oócitos. Observou-se superioridade do PSLE nosparâmetros VCL (PC-163,5μm/s, PSLE-186,2μm/s) e ALH (PC-9μm, PSLE- 8,2μm) do CASA, em relação ao controle. Na citometria de fluxo, o teste da anexina revelou maior quantidade de células viáveis não apoptóticas com o PSLE (38,9%) em relação ao TC (32,1%). No experimento 3 não houve diferença na prenhez após IA superficial (33,3%) e profunda (G2 52,2%), possivelmente devido ao número reduzido de animais
Raimundo, Inês da Silva. "O efeito de alguns factores na eficiência da inseminação artificial em tempo fixo em bovinos de carne." Master's thesis, Universidade de Lisboa. Faculdade de Medicina Veterinária, 2014. http://hdl.handle.net/10400.5/7880.
Full textA partir de 385 IATF realizadas em fêmeas bovinas de carne foram estudados os efeitos de diversos factores: tipo de inseminação artificial (IA), técnico de inseminação, intervalo entre a descongelação da palhinha de sémen e a deposição da dose no aparelho reprodutor da vaca, factores ambientais, número de partos e idade das vacas, intervalo parto-IA e raça na eficiência da técnica, medida objectivamente através da taxa de gestação. Todas as fêmeas foram sujeitas a um protocolo de sincronização de ovulação do tipo CO-Synch modificado, com recurso a um dispositivo intravaginal de libertação controlada de fármaco (CIDR) e gonadotrofina coriónica equina (eCG). A taxa de gestação foi calculada com base no diagnóstico de gestação, realizado através de ecografia cerca de 30 dias após a IA. Verificou-se que o tipo de IA (intracornual profunda, à entrada do corpo do útero ou cervical) não influenciou a taxa de gestação (p≥0,05), tal como o número de partos e idade da fêmea, intervalo parto-IA e a raça (p≥0,05). Os factores ambientais temperatura mínima, média e máxima, humidade relativa, velocidade do vento e precipitação também não exerceram influência na taxa de gestação (p≥0,05), tal como o índice temperatura-humidade (ITH), que relaciona temperatura e humidade relativa. Ao contrário de outros estudos, ITH>74 não foi responsável por quebras nas taxas de gestação (p≥0,05). Não foi possível estabelecer relação entre o técnico de inseminação e a eficiência da IATF em bovinos de carne (p≥0,05). Os factores intervalo descongelação-IA e traumatismo associado à IA influenciaram a taxa de gestação (p˂0,05). Concluiu-se que quanto maior o intervalo descongelação-IA, menor a probabilidade da fêmea bovina ficar gestante. Também a ocorrência de traumatismo diminuiu as hipóteses das vacas ficarem gestantes. Adicionalmente, registou-se evidência de relação entre estes dois factores.
ABSTRACT - THE EFFECT OF SOME FACTORS ON EFFICIENCY OF FIXED-TIME ARTIFICIAL INSEMINATION (FTAI) IN BEEF CATTLE - From 385 FTAI carried out on beef females some factors were studied like the type of artificial insemination (AI), insemination technician, interval between the thawig and the deposition of the semen on the reproductive tract of the cow (thawig-AI interval), environmental factors, parity and age of cows, calving-AI interval and breed, which may influence the fertility, measured objectively through the pregnancy rate. All bovine femals were synchronized for ovulation through a CO-Synch modified protocol, adding an intravaginal device controlled drug release (CIDR) and equine chorionic gonadotropin (eCG). The pregnancy rate was calculated on the basis of the pregnancy diagnosis, carried out through ultrasound approximately 30 days after AI. There was no difference (p≥0,05) between deep cornual inseminantion, conventional insemination (semen deposition into the uterine body) and cervical deposition. The cow age or parity, interval between calving and AI and the breed also did not influence the pregnancy rate (p≥0,05). Environmental factors like average, maximum and minimum daily temperatures, relative humidity, wind speed and precipitation also did not exert influence on the rate of pregnancy (p ≥ 0.05), as the temperature-humidity index (THI), which relates temperature and relative humidity. Unlike other studies, ITH > 74 was not responsible for decrease on the pregnancy rates (p ≥ 0.05). It had not been possible to establish if there was any relationship between the insemination technician and the efficiency of FTAI in beef cattle. The thawig-IA interval and trauma associated to AI influenced the pregnangy rate (p˂0,05). It was concluded that the higher the thawing-IA interval , the lower probability of the cow becoming pregnant. Also the occurrence of traumatic injuries didn’t favor the chances of bovine females becaming pregnant. In addition, we have found evidence of correlation between these two factors.
Baroncello, Edegar. "Inseminação artificial em tempo fixo em porcas desmamadas associada à utilização de análogo do GnRH e eCG." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2015. http://hdl.handle.net/10183/115187.
Full textFixed-time artificial insemination (FTAI) together with post-cervical artificial insemination (PCAI), allows an expressive reduction in labor requirements and a wider use of higher indexing boars. The aim of this study was to evaluate the efficiency of eCG agonist of GnRH (buserelin) or just buserelin for induction and synchronization of ovulation in weaned sows and submitted to FTAI .A total of 495 weaned sows whose estrus detection was performed once daily (07:30 A.M), starting a day after weaning. The sows were allocated into three treatment groups: Control (n=165) – the first AI was performed at estrus onset (0 h) and repeated every 24 h thereafter during estrus; Treatment 2: eCG + GnRH (n=165) – sows received an intramuscular injection of 600 UI eCG after weaning, and an intramuscular injection of buserelin (10 μg) 86-89 h after eCG administration; Treatment 3: GnRH - sows received an intramuscular injection of buserelina (10 μg) 86-89 h after weaning. . In the three groups it was performed boar exposure in the following day to weaning once a day, and those sows that demonstrated oestrus were removed from FTAI protocols. Treated groups received a single AI, 118-120 h after weaning (30-33 h after buserelin). PCAI with homospermic doses (1.5 x 109 total of sperm cells/50 ml) were respectively performed in sows in all treatment group. There were no differences (P> 0,05) in number of sows that showed oestrus until the third day between treatments. The interval between weaning and ovulation was significantly higher (P< 0,05) in the control group (141,5 ± 1,58 h) comparatively the treatments eCG+GnRH (133,3 ± 1,60 h) and GnRH (135,9 ± 1,57 h). Sows of treatment eCG+GnRH ovulated earlier (P< 0,05) comparing to the GnRH group (44,5 ± 1,74 vs 48,2 ± 1,73 h). Higher (P<0,05) farrowing rate in control group comparatively to treatments eCG+GnRH e GnRH was observed, but there were no differences (P> 0,05) in total piglets born between treatments. Use of GnRH, with or without previous administration of eCG anticipates ovulation in sows weaned, in the sows treated with eCG + GnRH or GnRH, there is a greater percentage of females inseminated outside the ideal time, with respect to ovulation, it can harm the reproductive performance, with necessary adjustments at the time of application hormones or at the time of insemination for subsequent studies.
Hallap, Triin. "Assessment of sperm attributes of frozen-thawed AI doses from Swedish and Estonian dairy bull sires : with special reference to pre-selection through swim-up, and the influence of age on potential fertility /." Uppsala : Dept. of Clinical Sciences, Swedish University of Agricultural Sciences, 2005. http://epsilon.slu.se/2005113.pdf.
Full textPinheiro, Carlos Bayard Martins. "Uso do semên ovino congelado em inseminação artificiais cervicais e fatores que afetam a fertilidade dos rebanhos." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2009. http://hdl.handle.net/10183/17455.
Full textA sheep improvement program depends on genetic connexion among flocks, which could be done by extensive use of frozen semen. However, nowadays-available technology just recommends the employment of frozen semen directly inside the uterine ambient through laparoscopy. Alternatively, there is a model recommended for Norway producers including frozen semen with 200 millions of sperms used in cervical superficial inseminations up to twelve hours from oestrus detection. The present essay is an local adaption of this system aiming to verify the viability of the employment of frozen semen for sheep reproduction in a volume of 0,25 ml, with 200 x 106 sperms in 0,50 ml straws, after natural oestrus, using low input resource and semi-qualified artificial insemination technicians. The observations were done in the two properties located at Rio Grande do Sul, including 1419 ewes from Merino, Ideal and Texel breeds. The results obtained indicate that the differences in non return rates among rams could be minimized by the employment of other methods of ram selection before the freezing procedures, and additionally the observed 25-35% non return rates in the distinct conditions investigated, permits to infer that the tested system could be useful to connect genetically the flocks using frozen semen.