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Academic literature on the topic 'CELLULE STAMINLI'

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Published: 10 March 2023

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Journal articles on the topic "CELLULE STAMINLI"

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Redi, Carlo Alberto, and Manuela Monti. "Clonazione e cellule staminali." SALUTE E SOCIETÀ, no. 3 (October 2010): 79–97. http://dx.doi.org/10.3280/ses2010-003006.

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Vandenbulcke, F., G. Beltrame, N. D. Vitale, B. Di Matteo, and E. Kon. "Le cellule staminali: impiego clinico." LO SCALPELLO-OTODI Educational 33, no. 3 (October 22, 2019): 237–42. http://dx.doi.org/10.1007/s11639-019-00345-9.

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Remuzzi, G. "Cellule Staminali: una Verità o Tante?" Giornale di Clinica Nefrologica e Dialisi 23, no. 1 (January 24, 2018): 44–45. http://dx.doi.org/10.33393/gcnd.2011.1461.

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Remuzzi, G. "Cellule Staminali: una Verità o Tante?" Giornale di Tecniche Nefrologiche e Dialitiche 23, no. 1 (January 2011): 44–45. http://dx.doi.org/10.1177/039493621102300112.

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Altavilla, Annagrazia, and Alessandro Dell’Erba. "La ricerca sulle cellule staminali: la nuova sfida dell’Europa unita." Medicina e Morale 53, no. 6 (December 31, 2004): 1133–78. http://dx.doi.org/10.4081/mem.2004.621.

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La ricerca sulle cellule staminali rappresenta uno dei settori più promettenti della biotecnologia, in quanto offre la possibilità di sviluppare nuovi metodi per riparare o sostituire le cellule o i tessuti lesionati o malati e per curare alcune patologie croniche gravi. Tale ricerca può anche fornire un contributo importante alla scienza di base, aiutando a comprendere i meccanismi di proliferazione e differenziazione cellulare. Gli embrioni umani preimpanto rappresentano una delle possibili fonti di cellule staminali. Tuttavia, laddove questa ricerca prevede l’utilizzo di embrioni umani, essa solleva la questione dei principi etici in gioco e dei limiti e delle condizioni cui questa deve essere soggetta. Gli stati europei hanno adottato posizioni diverse in merito alla regolamentazione della ricerca sulle cellule staminali embrionali. Tale disparità, che riflette le tradizioni etiche, filosofiche e religiose alle quali gli stati si ispirano, conferma l’esistenza di punti di vista divergenti in Europa su quanto sia o meno eticamente suscettibile di tutela. Questo articolo esamina le legislazioni e le posizioni etiche esistenti a tal proposito in Europa, oltre che i nuovi orientamenti sui principi da applicare nella concessione di finanziamenti comunitari (nell’ambito del VI Programma quadro di ricerca europeo –FP6) per progetti di ricerca implicanti l’uso di embrioni umani e di cellule staminali embrionali. Tale studio intende altresì fornire degli spunti di riflessione sui nuovi traguardi dell’integrazione europea nel settore della ricerca biomedica.
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Memeo, A., L. Pedretti, L. Rossi, F. Calabrò, and W. Albisetti. "L’utilizzo di cellule staminali in ortopedia pediatrica." Archivio di Ortopedia e Reumatologia 123, no. 3 (December 2012): 26–27. http://dx.doi.org/10.1007/s10261-012-0032-z.

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Capella, Vincente Bellver. "Luci e ombre nella ricerca con cellule staminali." Medicina e Morale 49, no. 5 (October 31, 2000): 851–67. http://dx.doi.org/10.4081/mem.2000.767.

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La Gran Bretagna ha recentemente aperto alla possibilità di clonare embrioni umani nella prospettiva di ottenere tessuti da trapiantare in malati per i quali vi sia una indicazione clinica. Gli Stati uniti, con lo stesso scopo, intendono finanziare la ricerca sugli embrioni soprannumerari derivanti dalle tecnologie di fecondazione artificiale. In questo lavoro, sostenuto dalla più recente letteratura disponibile, l’autore sostiene che esistono modalità di ottenimento dei tessuti meno controverse da punto di vista etico per raggiungere gli stessi obiettivi terapeutici e denuncia gli interessi che spingono ad offrire una informazione parziale su questa materia, peraltro assai rilevante per la medicina del futuro.
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Rizzo, Maria, Luigi Romano, and Nicola Tammaro. "Le cellule staminali mesenchimali nel trattamento delle pseudoartrosi." LO SCALPELLO-OTODI Educational 33, no. 3 (September 13, 2019): 270–74. http://dx.doi.org/10.1007/s11639-019-00328-w.

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Pellegrino, A., N. Tammaro, M. Conte, L. Romano, and S. Misso. "Il prelievo delle cellule staminali mesenchimali dalla cresta iliaca." LO SCALPELLO-OTODI Educational 33, no. 3 (September 12, 2019): 243–52. http://dx.doi.org/10.1007/s11639-019-00335-x.

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Battaglini, G., F. Guadalascara, and G. Monteleone. "Il prelievo delle cellule staminali mesenchimali dal tessuto adiposo." LO SCALPELLO-OTODI Educational 33, no. 3 (September 13, 2019): 253–57. http://dx.doi.org/10.1007/s11639-019-00342-y.

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Dissertations / Theses on the topic "CELLULE STAMINLI"

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Crespi, A. "Pacemaker biologico : cellule staminali embrionali murine vs. cellule staminali cardiache adulte." Doctoral thesis, Università degli Studi di Milano, 2008. http://hdl.handle.net/2434/56611.

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Recent progress in stem cell research makes it possible to use them in cardiac therapy. Our aim was to characterize the pacemaker current (If) and HCN channels distribution in murine embryonic stem cells and cardiac adult mesoangioblasts following differentiation towards a pacemaker-like phenotype. Mouse embryonic stem cells (mESCs) are pluripotent stem cells and they were differentiated through formation of embryoid bodies (EBs). A fraction of these cells exhibited action potentials characterized by a slow diastolic depolarization typical of pacemaker myocytes and expressed the If current. Immunofluorescence analysis revealed that both the HCN1 and HCN4 isoforms of the pacemaker channel are expressed. Rhythmic cells responded to β-adrenergic and muscarinic agonists: isoproterenol accelerated and acetylcholine slowed spontaneous rate. Accordingly, β1- and β2-adrenergic, and M2-muscarinic receptors were detected by immunofluorescence. Cardiac mesoangioblasts are vessel-associated clonogenic, self-renewing progenitor cells identified in the post-natal murine heart, which can be induced to differentiate into cardiac myocytes. A subpopulation of cardiac mesoangioblasts induced to differentiate in vitro into cardiomyocytes acquires a phenotype with specific pacemaker cells properties, such as the presence of If current and the expression of the HCN4 isoform of pacemaker channels. As in native cardiac pacemaker cells, f-channel modulation by autonomic transmitters, in these cells, contributes to control of spontaneous rate. These results show that both of these cell types express proteins which underlie generation and modulation of heart rhythm, and can represent a potential substrate for a biological pacemaker.
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Calvia, Valentina <1980&gt. "Cellule staminali, immortalità e enhancement." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2008. http://amsdottorato.unibo.it/665/1/Tesi_Calvia_Valentina.pdf.

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Calvia, Valentina <1980&gt. "Cellule staminali, immortalità e enhancement." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2008. http://amsdottorato.unibo.it/665/.

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Iacono, Eleonora <1976&gt. "Impiego di cellule staminali in terapia veterinaria." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2010. http://amsdottorato.unibo.it/2839/1/Iacono_Eleonora_Impiego_di_cellule_staminali_in_terapia_veterinaria.pdf.

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Over the past few years, in veterinary medicine there has been an increased interest in understanding the biology of mesenchymal stem cells (MSCs). This interest comes from their potential clinical use especially in wound repair, tissue engineering and application in therapeutics fields, including regenerative surgery. MSCs can be isolated directly from bone marrow aspirates, adipose tissue, umbilical cord and various foetal tissues. In this study, mesenchymal stem cells were isolated from equine bone marrow, adipose tissue, cord blood, Wharton’s Jelly and, for the first time, amniotic fluid. All these cell lines underwent in vitro differentiation in chondrocytes, osteocytes and adipocytes. After molecular characterization, cells resulted positive for mesenchymal markers such as CD90, CD105, CD44 and negative for CD45, CD14, CD34 and CD73. Adipose tissue and bone marrow mesenchymal stem cells were successfully applied in the treatment of tendinitis in race horses. Furthermore, for the first time in the horse, skin wounds of septicemic foal, were treated applying amniotic stem cells. Finally, results never reported have been obtained in the present study, isolating mesenchymal stem cells from domestic cat foetal fluid and membranes. All cell lines underwent in vitro differentiation and expressed mesenchymal molecular markers.
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Iacono, Eleonora <1976&gt. "Impiego di cellule staminali in terapia veterinaria." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2010. http://amsdottorato.unibo.it/2839/.

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Over the past few years, in veterinary medicine there has been an increased interest in understanding the biology of mesenchymal stem cells (MSCs). This interest comes from their potential clinical use especially in wound repair, tissue engineering and application in therapeutics fields, including regenerative surgery. MSCs can be isolated directly from bone marrow aspirates, adipose tissue, umbilical cord and various foetal tissues. In this study, mesenchymal stem cells were isolated from equine bone marrow, adipose tissue, cord blood, Wharton’s Jelly and, for the first time, amniotic fluid. All these cell lines underwent in vitro differentiation in chondrocytes, osteocytes and adipocytes. After molecular characterization, cells resulted positive for mesenchymal markers such as CD90, CD105, CD44 and negative for CD45, CD14, CD34 and CD73. Adipose tissue and bone marrow mesenchymal stem cells were successfully applied in the treatment of tendinitis in race horses. Furthermore, for the first time in the horse, skin wounds of septicemic foal, were treated applying amniotic stem cells. Finally, results never reported have been obtained in the present study, isolating mesenchymal stem cells from domestic cat foetal fluid and membranes. All cell lines underwent in vitro differentiation and expressed mesenchymal molecular markers.
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Calzarossa, C. "Terapia cellulare : potenzialità e applicazioni terapeutiche di cellule staminali fetali e adulte in modelli animali di neurodegenerazione." Doctoral thesis, Università degli Studi di Milano, 2008. http://hdl.handle.net/2434/61187.

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BOSSIO, CATERINA. "TRANSDIFFERENZIAMENTO DI CELLULE STAMINALI MESENCHIMALI DI RODITORE IN CELLULE DEL SISTEMA NERVOSO CENTRALE." Doctoral thesis, Università degli Studi di Milano, 2012. http://hdl.handle.net/2434/171967.

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One of the most important challenges in modern medicine is the identification of cell therapy protocols, enabling identification of personalized treatment strategies. WIthin this context, adult stem cells (ASC) have a large applicative potential. Contrary to ESCs (embryonic stem cells) and IPSCs (induced pluripotent stem cells), ASCs can be easily extracted from different tissues (bone marrow, skin, adipose tissue, muscle) without raising ethical issues . Moreover, they can be used for autologous transplantation, eliminating the complications associated with autoimmune reactions. Mesenchymal stem cells (MSC,), are an ASC population present in the bone marrow, adipose tissue and other tissues. MSCs are readily available and are able to self-replicate and differentiate, supported by the presence of appropriate stimuli, into cell lines derived from mesodermal lineage (osteoblasts, chondrocytes, adipocytes, and muscle cells). In the last decade it has also been observed that MSCs are able to trans-differentiate into cell types of ectodermal and endodermal origin (transdifferentiation).. The purpose of my work was to develop and define the conditions which can induce trans-differentiation of MSCs extracted from rat adipose tissue toward the neuronal phenotype. The study is part of a major project of regenerative medicine focused on identifying new strategies aimed at restoring functionality in degenerated brain tissue. Initially, MSCs have been characterized by the induction of osteoblastic differentiation, one of their physiological differentiations, and their interaction with biocompatible materials ( titanium dioxide) was analyzed, in order to assess their possible application in medicine for the creation, for example, of prothesic solutions. Subsequently, we sought to devise a GMP compliant cell culturing medium for MSC proliferation, crucial in order to obtain a clinically relevant cell number to differentiate following isolation from adipose tissue. For that reason we tried to induce the differentiation of MSCs into a neural phenotype; a first approach has been to apply protocols already known in literature for the differentiation of different types of stem cells toward the neuronal phenotype.. We considered both the direct differentiation protocols, as well as those that encompassed intermediate stages (sphere-forming). The protocols of differentiation by sphere formation resulted in differentiated MSC expressing glial markers (GFAP), but the protocol was too long(30 days) and a number of differentiated cells very low.. A second approach has been to develop a proprietary differentiation medium (NZ4) using the knowledge and expertise gained from the literature and from the negative results previously obtained The cells maintained in NZ4 differentiation medium , showed a clear morphological change and a high vitality; moreover they expressed both markers of specific neurons in the early stages of development (nestin, doublecortin) as well as markers expressed by mature neurons (βIIItubulin, VAMP2, Sinaptotagmin). Furthermore, the efficiency of the differentiation protocol has been functionally characterized by means of qualitative analysis of the dynamics of intracellular calcium and with quantitative analysis of the resting membrane potential The functional characterization clearly showed that MSC exposed to NZ4 differentiation medium have comparable behavior as primary neurons undergoing in vitro maturation. Given the future direction of the project will be to inject in an animal model the cell undergoing differentiation in order to attempt a partial recovery of the damaged neuronal tissue (i.e. ischemia) we characterized the behavior of MSC encapsulated in a biocompatible bioresorbable hydrogel matrix. The cells were encapsulated for different time periods, recovered, and heir survival as well as maintenance of stemness potential following retrieval was assessed . There are still other issues to be clarified n in particular, it will be necessary to verify in an in vivo setting the the efficacv of the differentiation protocol as well as to characterize the process of MSCs homing and to perform more detailed studies on behavioral changes of the same animal model. However, preliminary data obtained during this thesis allows to deepen the knowledge concerning the differentiation process of MSC from adipose tissue toward a neuronal phenotype.
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MAURI, MARIO. "Cellule staminali mesenchimali: potenziali modulatori del sistema nervoso centrale." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2012. http://hdl.handle.net/10281/39835.

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Bone marrow-derived mesenchymal stem cells (MSCs) account for a small population of cells of the non-hematopoietic component of bone marrow. MSCs are multipotent stem cells endowed with neurotrophic potential combined to immunological properties, making them a promising therapeutic tool for neurodegenerative disorders. Although the mechanisms by which they act are still largely unknown, trans-differentiation, paracrine and autocrine actions have been hypothesized. Here we focus on the study of the effects exerted by rat MSCs on CNS neurons and oligodendrocytes by using a simplified in vitro co-culture system that precludes any direct contact between different cell types. The analysis of hippocampal synaptogenesis, synaptic vesicle recycling and electrical activity show that MSCs by themselves, efficiently support morphological and functional neuronal differentiation. Our observations demonstrate that MSCs selectively and directly increased hippocampal GABAergic presynapses and inhibitory transmission. In fact, this increment correlated to a higher expression of the potassium/chloride KCC2 cotransporter and to an enhancement of both the frequency and the amplitude of mIPSC and sIPSC. The decreased of GABA synapses following the treatment with a widely used Trk-neurotrophin receptor blocker, K252a, and the more specific TrkB receptor bodies prompt for the involvement of the brain derived neurotrophic factor (BDNF) in mediating such effects. The involvement of this neurotrophin is also strengthened by test ELISA on the culture medium collected from MSC-neuron co-cultures in which an higher BDNF concentration was detected, when compared to astrocyte-neuron co-cultures. The results obtained indicate that MSC-secreted factors induce glial-dependent neuronal survival and directly trigger an augmented GABAergic transmission in hippocampal cultures, highlighting a new effect by which MSCs could cooperate in CNS repair. Additionally, MSCs have been described to improve the clinical course of some demyelinating pathologies and to promote tissue repair through immunological mechanisms and neuroprotective effects. Following these evidences we performed in vitro and in vivo experiments to assess whether MSCs exert their actions through the support of oligodendrocytes (OLs), the myelinating CNS cells, and participate in the regulation of their proliferation and maturation. Through the analysis of specific proteins typically used as markers of the different stages of proliferation, maturation and differentiation (specifically, the membrane glycoprotein O4, the proteoglycan NG2 and myelin basic protein MBP, respectively), it has been noticed that MSCs are capable to prolong the proliferation phase of OPCs and also to anticipate OL differentiation, with respect to standard astrocyte/OL co-cultures. Moreover we investigated a possible molecular mechanism underlying these phenomena focusing on neurotrophin pathways. Trk receptors activation was analyzed in order to find out a possible role of neurotrophins in MSC-mediated effects on OLs, as it happens in neuronal cultures. We focused on the changes in the phosphorylation level of ERK (Extracellular signaling-regulated kinases), one of the activated effectors by TrK receptors. Our observations show that, in OLs co-cultured with MSCs, ERK is highly phosphorylated with respect to astrocyte/OL co-cultures, suggesting a MSC-induced activation of the pathways regulated by this protein. These data, although preliminary, suggest that MSCs positively act on the regulation of proliferation and maturation of OLs and, due to the observed effects on the regulation of synaptogenesis (see above), make these cells an interesting model for the identification of molecules involved in MSC neuroprotective processes. This may open new therapeutic approaches in the treatment of neurodegenerative diseases involving not only a synaptic imbalance, as it happens in various forms of epilepsy, but also in demyelinating diseases. Thus, in this research project, we aimed at characterising the molecular mechanisms underlying MSC actions that could participate in the recovery of neurological disorders or demyelinating pathologies.
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Ditadi, Andrea. "Approccio di terapia cellulare mediante l'utilizzo di cellule fetali isolate dal liquido amniotico per malattie del sistema ematopoieico." Doctoral thesis, Università degli studi di Padova, 2008. http://hdl.handle.net/11577/3425090.

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Cell therapy is an attractive perspective for the treatment of life threatening disorders. In this context, foetal tissues are gaining interest as sources of cells for auto- and allo-transplantation, because of their pluripotency, proliferative capability and their low, if any, immunogenicity. Recently a pluripotent stem cell population has been isolated from Amniotic Fluid (AF). It is able to proliferate for more than 18 months, maintaining their differentiative ability as well as a normal karyotype. In term of differentiation potential, we succeeded in obtaining in vitro mesenchymal-, ectodermal- and endodermal-derived tissues from human Amniotic Fluid Stem (AFS) cells. Furthermore, murine AFS cells injected in blastocytes took part to the formation not only of several different foetal organs, but also of the placenta and the umbilical cord. In the present study we investigated the possibility of differentiating AFS cells towards the hematopoietic pathway. AFS cells isolated from human amniotic fluid, collected during routine diagnostic procedures and obtained under informed consent, were firstly expanded in vitro and selected on the basis of their ckit expression. We achieved a reproducible erythroid differentiation by culturing hAFSCs as embryoid bodies (EBs) under serum free conditions with haematopoietic cytokines. Erythroid cells expressing CD235a constituted 70% of the total hAFSCs forming EBs showing also a co-expression of CD36 and CD71. Furthermore, human erythrocytes (human CD235a) were isolated from bone marrow and spleen of sublethally irradiated NOD/SCID mice at 3 months after the injection of hAFSCs. To determine if the expansion procedure had led to a restriction of the hematopoietic potential towards the erythroid pathway, we compared expanded AFSCs and freshly isolated cKit+ Lin- (AFKL) cells. We also harvested cKit+ Lin- KL cells from the membrane surrounding the AF, the Amnion, in search for a possible origin. We compared the hematopoietic potential of mAFKL and mAmKL to Fetal Liver KL, the main source of fetal HSC. When cultivated immediatly after their sorting, freshly isolated murine AFKL and AmKL cells gave rise to all the different hematopoietic lineages both in vitro and in vivo. Actually, when cocultivated with OP9(d)1 cells, AFKL and AmKL undergo complete T cell differentiation within 2 weeks. They also generate myeloid and erythroid colonies when cultivated in methylcellulose for clonogenic assay. The erythroid restricted potential of human AFS cells was thus probably linked to the in vitro expansion procedure. Moreover, cells belonging to all the three hematopoietic lineages (lymphoid, myeloid and erythroid) and arising from freshly isolated mAFKL and mAmKL are found in the peripheral blood of sublethally irradiated RAG1 deficient mice only 4 weeks after transplantation. Four month later, transplanted mice showed mAFKL-derived lymphoid, myeloid and erythroid cells, in all the hematopoietic organs. Successful econdary transplantation strongly suggest that mAFKL and mAmKL comprise HSC, with self-renewal ability. Those results were very similar to those obtained with mFLKL, confirming the strong hematopoietic potential of mAFKL and mAmKL. Experiments with freshly isolated hAFKL gave good results in the in vitro assays being able to give rise to erythroid, myeloid and lymphoid lineages, but failed to reconstitute the hematopoietic system in irradiated NOD/SCID mice, probably due to the poor amount of cells injected. This is the first report demonstrating that AFKL and AmKL do have an haematopoietic potential, supporting the idea that AF and Am may be an excellent source for therapeutic application.
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Maurizi, Giulia. "Caratterizzazione fenotipica, molecolare e proprietà immunoregolatorie delle cellule staminali mesenchimali." Doctoral thesis, Università Politecnica delle Marche, 2012. http://hdl.handle.net/11566/242057.

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Le cellule staminali mesenchimali (Mesenchymal Stem Cells, MSCs) sono una popolazione con elevata capacità proliferativa e con potenziale di differenziazione multilineare; rappresentano, quindi, delle buone candidate per la terapia cellulare e la medicina rigenerativa. In questo studio sono state valutate MSCs fetali isolate da villi coriali (Chorionic Villi, CV) e da liquido amniotico (Amniotic Fluid, AF), confrontandole con le MSCs ottenute da midollo osseo (Bone Marrow, BM), per la capacità di crescita in presenza di siero umano allogenico (human serum, HS) o di lisato piastrinico (platelet lysate, PL), per le caratteristiche immunofenotipiche, il profilo di espressione citochinico e l’attività immunoregolatoria su linfociti allogenici stimolati e sottopopolazioni immunoselezionate. Data l’elevata potenzialità di espansione delle MSCs fetali, le cellule studiate sono state valutate per la loro stabilità replicativa tramite lo studio della lunghezza dei telomeri, l’attività dell’enzima telomerasi, l’espressione dei geni hTERT, c-myc e p53 e l’analisi del cariotipo. Una popolazione omogenea di cellule fibroblastoidi positiva ai tipici marcatori di superficie mesenchimali è stata isolata da tutti i campioni di CV ed AF analizzati. Le cellule di CV espandono rapidamente in HS (20 raddoppiamenti di popolazione, PDs, in 59 giorni e 6 passaggi di coltura), mentre in siero animale (fetal bovine serum, FBS) raggiungono 27 PDs in 65 giorni e 7 passaggi. Il PL determina un’espansione nel 60% dei campioni CV, comunque minore rispetto a quella in HS. I campioni di AF espandono, invece, 40 PDs in 90 giorni, ma solo nel 20% dei campioni analizzati, non proliferano in PL, mentre in FBS espandono 28.5 PDs in 66 giorni. Le cellule fetali studiate inibiscono la proliferazione di linfociti allogenici stimolati e regolano la crescita anche di popolazioni linfocitarie selezionate CD4+ e CD8+. Nonostante il loro elevato potenziale di espansione, le MSCs fetali studiate hanno mostrato una lunghezza stabile dei telomeri durante la cultura a lungo termine, assenza dell’attività telomerasica, nessuna espressione di hTERT, livelli costanti di espressione di c-myc e p53 e nessuna anomalia cromosomica. In conclusione, i risultati mostrano che i CV rappresentano un’ottima fonte di MSCs con proprietà immunoregolatorie, capace di espandere in un sistema di proliferazione umanizzato a lungo termine senza alterazioni genetiche. In più del 90% dei campioni di CV analizzati si ottiene un’espansione su larga scala in presenza di siero umano, incoraggiante per potenziali applicazioni cliniche.
Mesenchymal stem cells (MSCs) are promising candidates for cell therapy and tissue engineering. In previous studies, the most important source of MSCs was adult bone marrow (BM). Recently, MSCs with similar cell surface markers and differentiation capacity have also been found in developmentally younger tissues. This study showed data from fetal MSCs obtained from first-trimester chorionic villi (CV) and second trimester amniotic fluid (AF), comparing them with BM. The work reports on growth in human allogeneic serum (HS) and platelet lysate (PL), immunophenotype, cytokine expression profile and immunoregulatory activity of fetal MSCs on stimulated peripheral blood mononuclear lymphocyte subpopulations. The cells studied was analyzed also for telomere length, telomerase activity, hTERT, p53 and cmyc transcriptions, to evaluate their replicative stability. Spontaneous chromosomal alterations were excluded by cytogenetic analysis. CV cells grow rapidly in HS, with 20 populations doublings (PDs) after 59 days (6 passages), and also in animal serum, with 27 PDs after 65 days (7 passages). PL allowed an expansion in 60% of the samples tested, though it was lower than HS. HS supported an average of 40 PDs of expansion in 20% of AF cells after 90 days, whereas animal serum supported 28.5 PDs in 66 days. CV and AF cells inhibited the proliferation of stimulated T lymphocytes, suppressing the growth of both CD4+ and CD8+ T subpopulations and, sometimes, CD19+ cells. Despite their high proliferation capacity, fetal MSCs showed no telomerase activity, no hTERT transcriptions and maintained long, stable telomeres. A constant expression level of p53 and c-myc and a normal karyotype were preserved throughout long-term expansion, suggesting the safety of fetal MSCs. In conclusion, these results indicate that CV would be an optimal and safety source of MSCs with high expansion potential in a HS propagation system, immunoregulatory capacity of T and B lymphocytes. More than 90% of CV samples achieved a large-scale expansion in HS, that is encouraging for potential clinical applications of these cells.
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Books on the topic "CELLULE STAMINLI"

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Puca, Antonio. Cellule staminali: Sfide e prospettive. Marigliano (Na) [i.e. Naples, Italy]: LER, 2011.

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Milano, Gianna. La rivoluzione delle cellule staminali. Milano: Feltrinelli, 2005.

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Rollier, Anna. Cellule staminali: Aspetti scientifici e questioni etiche. Torino: Claudiana, 2010.

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Leone, Salvino. Cellule staminali: Speranze terapeutiche e problemi etici. Assisi: Cittadella, 2011.

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Neri, Demetrio. La bioetica in laboratorio: Cellule staminali, clonazione e salute umana. Roma: Laterza, 2001.

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Neri, Demetrio. La bioetica in laboratorio: Cellule staminali, clonazione e salute umana. Roma [etc.]: GLF editori Laterza, 2001.

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Semprebon, Gabriele. Le cellule staminali e l'embrione: Elementi biologici e questione etica. Bologna: EDB Edizioni Dehoniane Bologna, 2015.

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Staminalia: Le cellule etiche e i nemici della ricerca. Parma: U. Guanda, 2008.

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Lucio, Militerni, and Veronesi Umberto, eds. Cellule staminali: Etica e qualità di vita : normativa europea e legislazione internazionale. Milanofiori Assago (Milano): UTET giuridica, 2012.

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L'officina della vita: Cellule staminali, medicina rigenerativa, trapianti : come si ripara il corpo umano. [Milano]: Garzanti, 2002.

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Book chapters on the topic "CELLULE STAMINLI"

1

Pescatori, Mario. "Cellule staminali." In Ascessi, fistole anali e retto-vaginali, 61–62. Milano: Springer Milan, 2011. http://dx.doi.org/10.1007/978-88-470-1914-0_22.

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2

D’Amato, Luca Colucci, and Umberto di Porzio. "Le cellule staminali neurali." In Introduzione alla neurobiologia, 91–103. Milano: Springer Milan, 2011. http://dx.doi.org/10.1007/978-88-470-1944-7_7.

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3

Alberto Redi, Carlo. "Dalla descrizione alla sintesi del vivente: Cellule staminali e biologia sintetica." In Frontiere mobili, 49–65. Mimesis Edizioni, 2014. http://dx.doi.org/10.4000/books.mimesis.2813.

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