Log in

Relevant bibliographies by topics / Cell hybridisation / Journal articles

Journal articles on the topic 'Cell hybridisation'

To see the other types of publications on this topic, follow the link: Cell hybridisation.

Author: Grafiati

Published: 4 June 2021

Last updated: 5 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the top 50 journal articles for your research on the topic 'Cell hybridisation.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.

1

Harrison, R. H., H. C. Kuo, P. N. Scriven, A. H. Handyside, and C. Mackie Ogilvie. "Lack of cell cycle checkpoints in human cleavage stage embryos revealed by a clonal pattern of chromosomal mosaicism analysed by sequential multicolour FISH." Zygote 8, no. 3 (August 2000): 217–24. http://dx.doi.org/10.1017/s0967199400001015.

Full text

Abstract:

Multicolour fluorescence in situ hybridisation (FISH) analysis of interphase nuclei in cleavage stage human embryos has highlighted a high incidence of postzygotic chromosomal mosaicism, including both aneuploid and ploidy mosaicism. Indeed, some embryos appear to have a chaotic chromosomal complement in a majority of nuclei, suggesting that cell cycle checkpoints may not operate in early cleavage. Most of these studies, however, have only analysed a limited number of chromosomes (3–5), making it difficult to distinguish FISH artefacts from true aneuploidy. We now report analysis of 11 chromosomes in five sequential hybridisations with standard combinations of two or three probes and minimal loss of hybridisation efficiency. Analysis of a series of arrested human embryos revealed a generally consistent pattern of hybridisation on which was superimposed frequent deletion of one or both chromosomes of a specific pair in two or more nuclei indicating a clonal origin and continued cleavage following chromosome loss. With a binucleate cell in a predominantly triploid XXX embryo, the two nuclei remained attached during preparation and the chaotic diploid/triphoid status of every chromosome analysed was the same for each nucleus. Furthermore, in each hybridisation the signals were distributed as a mirror-image about the plane of attachment, indicating premature decondensation during anaphase consistent with a lack of checkpoint control.

APA, Harvard, Vancouver, ISO, and other styles

2

Toebe, Kerstin. "Whole cell hybridisation for monitoring harmful marine microalgae." Environmental Science and Pollution Research 20, no. 10 (July 9, 2013): 6816–23. http://dx.doi.org/10.1007/s11356-012-1416-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Howroyd, Simon, and Rui Chen. "Powerpath controller for fuel cell & battery hybridisation." International Journal of Hydrogen Energy 41, no. 7 (February 2016): 4229–38. http://dx.doi.org/10.1016/j.ijhydene.2016.01.038.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Partridge, Wu, and Bucknall. "Investigation on the Impact of Degree of Hybridisation for a Fuel Cell Supercapacitor Hybrid Bus with a Fuel Cell Variation Strategy." Vehicles 2, no. 1 (December 19, 2019): 1–17. http://dx.doi.org/10.3390/vehicles2010001.

Full text

Abstract:

This paper presents the development of a control strategy for a fuel cell and supercapacitor hybrid power system for application in a city driving bus. This aims to utilise a stable fuel cell power output during normal operation whilst allowing variations to the power output based on the supercapacitor state-of-charge. This provides flexibility to the operation of the system, protection against over-charge and under-charge of the supercapacitor and gives flexibility to the sizing of the system components. The proposed control strategy has been evaluated using validated Simulink models against real-world operating data collected from a double-decker bus operating in London. It was demonstrated that the control strategy was capable of meeting the operating power demands of the bus and that a wide range of degrees of hybridisation are viable for achieving this. Comparison between the degree of hybridisation proposed in this study and those in operational fuel cell (FC) hybrid buses was carried out. It was found that the FC size requirement and FC variation can be significantly reduced through the use of the degree of hybridisation identified in this study.

APA, Harvard, Vancouver, ISO, and other styles

5

Bickmore, W. A., and A. D. Carothers. "Factors affecting the timing and imprinting of replication on a mammalian chromosome." Journal of Cell Science 108, no. 8 (August 1, 1995): 2801–9. http://dx.doi.org/10.1242/jcs.108.8.2801.

Full text

Abstract:

Fluorescence in situ hybridisation has been used to follow replication of the short arm of human chromosome 11 using chromosome anomalies to distinguish the maternally-and paternally-derived homologues. The temporal difference in replication timing within and between chromosomes has been estimated by combining S phase detection with dual colour fluorescence in situ hybridisation. Proximal regions of 11p, including the WT1 gene, tend to replicate earlier on the maternally-derived chromosome than on the paternally-derived homologue. More distal parts of 11p (including the IGF2 gene) have the opposite imprint. The average difference in replication timing between homologous loci in the population of cells is small compared to the differences between loci along a single chromosome. The imprint is not strictly adhered to since many nuclei have hybridisation patterns opposite to the trend within the population. The nature of the imprinting signal has been investigated. Absolute replication time, but not the imprint, was affected by azacytidine, an inhibitor of DNA methylation. The replication imprint was modified by treatments that inhibit histone deacetylation. We suggest that replication imprinting reflects differences in chromatin structure between homologues.

APA, Harvard, Vancouver, ISO, and other styles

6

Tholouli, Eleni, Dolores Di Vizio, Fionnuala O’Connell, Massimo Loda, David Twomey, Todd Golub, Richard Levenson, Judith A. Hoyland, John A. L. Yin, and Richard Byers. "Quantum Dot Based Duplex In Situ Hybridisation for Gene Expression Profiling." Blood 106, no. 11 (November 16, 2005): 3265. http://dx.doi.org/10.1182/blood.v106.11.3265.3265.

Full text

Abstract:

Abstract Quantum dots (QDs) are fluorescent semiconductor nanocrystals (2–10-nm core diameter) possessing the unique properties of extremely high fluorescence efficiency, lack of photobleaching and long fluorescence lifetime, making them an ideal tool for bioimaging. We have developed a novel technique for in situ hybridisation (ISH) using biotinylated oligonucleotides conjugated to streptavidin coated QD, and used them in this study to label bone marrow trephine samples. 50-mer long oligonucleotide probes were conjugated to QDs prior to ISH and conjugation efficiency was demonstrated by gel electrophopresis. ISH conditions and molar ratio of QDs to probe were optimised using a polyT probe. Images were captured using a CRI Nuance spectral imaging system and signal intensity was semi-quantitated using IPLab software. Specific oligonucleotide hybridisation was demonstrated using a probe for myeloperoxidase (MPO) in 40 bone marrow sections infiltrated by acute myeloid leukaemia (AML), acute lymphoblastic leukaemia (ALL), chronic myeloid leukaemia (CML) as well as reactive bone marrow. In each case hybridisation signal was consistent with the distribution of MPO by standard immunohistochemistry - MPO was strongly expressed by myeloid blasts and absent in lymphoid blasts; in CML, most, but not all, cells were positive for MPO, in comparison to many fewer positive cells in reactive marrow. Duplex ISH was demonstrated using a probe for bcl-2 together with MPO in 5 bone marrow sections infiltrated by follicular lymphoma (FL). Strong hybridisation signal for bcl-2 was detected in all cells of the paratrabecular aggregates of FL but showed only scattered positivity in the remainder of the bone marrow. Conversely, MPO was absent in the paratrabecular aggregates and present in the myeloid cells in the remainder of the marrow. This pattern was present in both single and duplex ISH for MPO and bcl-2 in the marrow infiltrated by FL. Duplex ISH was performed both by sequential hybridisation with bcl-2 followed by MPO, and simultaneously with a mix of bcl-2 and MPO probes. As negative controls, scrambled oligonucleotide probes for the corresponding genes were used in each case and did not show hybridisation. In summary, we have developed a generic method for QD labelling and semi-quantitative detection of oligonucleotide ISH in routinely processed clinical tissue samples. Although, in this study we primarily used bone marrow trephine samples, this technique can be applied to any tissue. It has the potential to facilitate transfer of microarray-identified gene signatures to clinical research and diagnostics, whilst the ability of spectral imaging to resolve multiple signals offers the possibility of multiplexed probe detection.

APA, Harvard, Vancouver, ISO, and other styles

7

MCFADDEN, G. "In situ hybridisation in plants: From macroscopic to ultrastructural resolution." Cell Biology International Reports 13, no. 1 (January 1989): 3–21. http://dx.doi.org/10.1016/s0309-1651(89)80004-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Hu, Eddie, James Thompson, Sandra Horning, Martha Trela, James Lowder, Ronald Levy, and Jeffrey Sklar. "DETECTION OF B-CELL LYMPHOMA IN PERIPHERAL BLOOD BY DNA HYBRIDISATION." Lancet 326, no. 8464 (November 1985): 1092–95. http://dx.doi.org/10.1016/s0140-6736(85)90686-5.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Marchio, A. "Chromosomal abnormalities in liver cell dysplasia detected by comparative genomic hybridisation." Molecular Pathology 54, no. 4 (August 1, 2001): 270–74. http://dx.doi.org/10.1136/mp.54.4.270.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Gavins, Georgina C., Katharina Gröger, Marc Reimann, Michael D. Bartoschek, Sebastian Bultmann, and Oliver Seitz. "Orthogonal coiled coils enable rapid covalent labelling of two distinct membrane proteins with peptide nucleic acid barcodes." RSC Chemical Biology 2, no. 4 (2021): 1291–95. http://dx.doi.org/10.1039/d1cb00126d.

Full text

Abstract:

A pair of orthogonal coiled coils templates highly specific live cell bioconjugation of two different proteins. PNA tagging and hybridisation with fluorophore–DNA reporters enables rapid dual receptor internalisation analysis of EGFR and ErbB2.

APA, Harvard, Vancouver, ISO, and other styles

11

Meier, H., C. Koob, W. Ludwig, R. Amann, E. Frahm, S. Hoffmann, U. Obst, and K. H. Schleifer. "Detection of enterococci with rRNA targeted DNA probes and their use for hygienic drinking water control." Water Science and Technology 35, no. 11-12 (June 1, 1997): 437–44. http://dx.doi.org/10.2166/wst.1997.0774.

Full text

Abstract:

Enterococci are useful indicators of faecal contamination with their high abundance in faeces and long survival in the environment and the possibility of indicating the source of contamination by species identification has lead to discussion of whether enterococci would be more reliable faecal indicators than E. coli. In an attempt to facilitate rapid and accurate identification of enterococci, 16S rRNA targeted oligonucleotide probes were designed by computer-aided analysis of more than 4,000 rRNA sequences. Probes were labelled non-isotopically with digoxigenin and fluorescent dyes. Conditions for specific hybridisation were optimised for dot blot hybridisation and whole cell hybridisation for all probes. With a combination of two probes, all hygienically important enterococci could be detected and 24 biochemically identified environmental isolates also hybridised with one of these probes. A quantitative detection method with a high sensitivity was developed based on filtration of water samples through polycarbonate filters, a short incubation on agar and microcolony filter hybridisation with fluorescently labelled probes followed by epifluorescence microscopy. Within 8–20h sampling a specific identification of enterococcal microcolonies was possible. With this method 15/32 well- and tap-water sources from the Mainz area were identified as being of substandard quality. The proposed method detects faecal contamination significantly earlier than conventional methods and could be helpful in the hygienic monitoring of drinking water.

APA, Harvard, Vancouver, ISO, and other styles

12

Hartt, L. S., S. J. Carling, M. M. Joyce, G. A. Johnson, D. K. Vanderwall, and T. L. Ott. "Temporal and spatial associations of oestrogen receptor alpha and progesterone receptor in the endometrium of cyclic and early pregnant mares." Reproduction 130, no. 2 (August 2005): 241–50. http://dx.doi.org/10.1530/rep.1.00596.

Full text

Abstract:

Uterine function is primarily controlled by the combined actions of oestrogen and progesterone working through their cognate nuclear receptors. The mechanism of establishment of pregnancy in the mare is of interest because it involves prolonged pre-attachment and conceptus migration phases, and both invasive and non-invasive placental cell types, and as such has been an important comparative model. This study characterised regulation of oestrogen (ER) and progesterone (PR) receptors in the endometrium of the mare during the oestrous cycle and early pregnancy. Endometrial tissues collected during the oestrous cycle and early pregnancy were analysed for steady-state levels of ER and PR mRNA and protein. Steady-state levels of ER and PR mRNA were highest on days 0, 17 and 20 in cyclic mares and lowest on days 11 and 14. A day-by-status interaction was detected, indicating that day 17 and day 20 pregnant mares exhibited low levels of ER and PR compared with the corresponding days of the oestrous cycle. In situ hybridisation analyses showed receptor mRNA localisation primarily in the luminal epithelium (LE), glandular epithelium (GE) and stroma around oestrus. During dioestrus and early pregnancy, receptors were not detected in the LE, and were lower in the stroma and deeper GE. Changes in hybridisation intensity in these cell types were consistent with changes in mRNA levels detected by slot-blot hybridisation. ER and PR proteins were detected in the nuclei of LE, GE and stromal cells. Consistent with results from in situ hybridisation, levels of ER and PR immunoreactivity were higher around oestrus, declined to low levels during dioestrus and remained low during early pregnancy. Results described here for temporal and spatial changes in steroid receptor gene expression in mares show the greatest similarities with those described for cattle and sheep.

APA, Harvard, Vancouver, ISO, and other styles

13

Ward, Rita D., Lisa M. Mendoza, Gary W. Moy, Victor D. Vacquier, and David Nishioka. "A unique expression pattern for a sperm membrane protein during sea urchin spermatogenesis." Zygote 2, no. 2 (May 1994): 159–65. http://dx.doi.org/10.1017/s096719940000191x.

Full text

Abstract:

SummarySpecific mRNAs coding for a 63 kDa sperm membrane protein (63-SMP) were localised in Strongylocentrotus purpuratus testis sections using in situ hybridisation techniques. 35S-labelled antisense RNA probes transcribed from a 766 base pair fragment of the gene coding for the 63-SMP hybridised to all spermatogenic cells in the basal germinal epithelia of testicular acini, except the most peripherally located (least differentiated) spermatogonia. No hybridisation to the luminally located mature spermatozoa or somatic cells of the testis was observed. Using monoclonal antibody J17/30 and indirect immunofluorescence techniques, the 63-SMP was localised to the same subset of spermatogenic cells that contain the 63-SMP mRNA, suggesting that expression of this gene is transcriptionally controlled. In combination with previous studies on the expression of sperm histones and sperm bindin, these results show that multiple, perhaps sequential, classes of gene activity contribute to the differentiation of sea urchin sperm.

APA, Harvard, Vancouver, ISO, and other styles

14

Ochatt, Sergio. "Plant cell electrophysiology: Applications in growth enhancement, somatic hybridisation and gene transfer." Biotechnology Advances 31, no. 8 (December 2013): 1237–46. http://dx.doi.org/10.1016/j.biotechadv.2013.03.008.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Hamid, Q., M. M. Kelly, M. Linden, R. Louis, M. M. M. Pizzichini, E. Pizzichini, C. Ronchi, F. Van Overveld, and R. Djukanovic. "Methods of sputum processing for cell counts, immunocytochemistry and in situ hybridisation." European Respiratory Journal 20, Supplement 37 (July 1, 2002): 19S—23s. http://dx.doi.org/10.1183/09031936.02.00001902.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Dent, J., G. D. Hall, N. Wilkinson, T. J. Perren, I. Richmond, A. F. Markham, H. Murphy, and S. M. Bell. "Cytogenetic alterations in ovarian clear cell carcinoma detected by comparative genomic hybridisation." British Journal of Cancer 88, no. 10 (May 2003): 1578–83. http://dx.doi.org/10.1038/sj.bjc.6600896.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Nesbit, M. A., M. Helfrich, and M. A. Horton. "Osteoclast cell matrix receptor and ligand expression detected by in situ hybridisation." Bone 13, no. 1 (1992): 102. http://dx.doi.org/10.1016/8756-3282(92)90388-d.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Medlin, Linda K., and Sabine Strieben. "Refining cryptophyte identification: matching cell fixation methods to FISH hybridisation of cryptomonads." Journal of Applied Phycology 22, no. 6 (March 18, 2010): 725–31. http://dx.doi.org/10.1007/s10811-010-9512-z.

Full text

APA, Harvard, Vancouver, ISO, and other styles

19

Schedle, A., M. Willheim, A. Zeitelberger, A. Gessl, K. Frauendorfer, C. Sch�fer, F. Wachtler, H. G. Schwarzacher, and G. Boltz-Nitulescu. "Nucleolar morphology and rDNA in situ hybridisation in monocytes." Cell & Tissue Research 269, no. 3 (September 1992): 473–80. http://dx.doi.org/10.1007/bf00353902.

Full text

APA, Harvard, Vancouver, ISO, and other styles

20

Pandey, KK, JE Grant, and EG Williams. "Interspecific Hybridisation Between Trifolium repens and T. uniflorum." Australian Journal of Botany 35, no. 2 (1987): 171. http://dx.doi.org/10.1071/bt9870171.

Full text

Abstract:

Several partially fertile hybrids (2n = 32) were produced by embryo culture from crosses of the type T. repens (2n = 32) female × T. uniflorum (2n = 32) male . The reciprocal crosses, although giving better seed development in vivo, were less successful in producing viable hybrid plants. Backcrosses to both parent species and F2 hybrids were also produced. Hybrid materials were variable with respect to morphological characteristics but broadly within the expected intermediate range. Their root systems were generally coarser and deeper than that of T. repens, offering the prospect of improved resistance to beetle larvae and drought. One F1 hybrid proved to be highly self-compatible, although derived from self-incompatible parent species. Pollen fertility ranged from 0 to 58% for F1 hybrids, 0 to 13% for F2 hybrids, 0 to 84% for backcrosses to T. repens and 0 to 26% for backcrosses to T. uniflorum. Marked seasonal variations in pollen fertility were also observed. Up to two quadrivalent chromosome associations per pollen mother cell were observed at meiosis in F1 hybrids, indicating some intragenomic pairing of T. repens chromosomes. Quadrivalent associations were also observed in an F2 hybrid and three backcrosses to T. repens.

APA, Harvard, Vancouver, ISO, and other styles

21

Hopman, A. H. N., Sandra Claessen, and Ernst J. M. Speel. "Multi-colour brightfield in situ hybridisation on tissue sections." Histochemistry and Cell Biology 108, no. 4-5 (October 17, 1997): 291–98. http://dx.doi.org/10.1007/s004180050168.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Eleuteri, P., M. G. Grollino, D. Pomponi, and R. De Vita. "Chromosome 9 aberrations by fluorescence in situ hybridisation in bladder transitional cell carcinoma." European Journal of Cancer 37, no. 12 (August 2001): 1496–503. http://dx.doi.org/10.1016/s0959-8049(01)00151-4.

Full text

APA, Harvard, Vancouver, ISO, and other styles

23

Rickert, Christian H., and Werner Paulus. "No chromosomal imbalances detected by comparative genomic hybridisation in subependymal giant cell astrocytomas." Acta Neuropathologica 104, no. 2 (May 29, 2002): 206–8. http://dx.doi.org/10.1007/s00401-002-0544-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Miah, M. S., S. Majumdar, S. White, M. Robinson, and N. Kernohan. "Human papillomavirus and salivary gland neoplasia: a p16INK4 immunohistochemical and in situ hybridisation study." Journal of Laryngology & Otology 129, no. 10 (July 20, 2015): 1000–1003. http://dx.doi.org/10.1017/s0022215115001851.

Full text

Abstract:

AbstractObjective:This study aimed to evaluate the association between human papillomavirus infection and salivary gland tumours in a Scottish cohort.Methods:Specimens from a range of salivary gland tumours operated on between 1997 and 2012 were studied. A tissue microarray constructed from tissue blocks was subjected to p16INK4 (cyclin-dependent kinase inhibitor 2A) immunohistochemistry and in situ hybridisation using probes specific for human papillomavirus, including types 16 and 18.Results:A total of 61 tumours (benign and malignant) were deemed suitable for the study. p16INK4 staining yielded three (4.9 per cent) positive samples: one small cell carcinoma, one squamous cell carcinoma and one poorly differentiated carcinoma. Human papillomavirus in situ hybridisation demonstrated a positive signal in the latter sample only (1.6 per cent).Conclusion:This study demonstrated a very low human papillomavirus detection rate in salivary gland tumours. It can therefore be concluded that human papillomavirus infection is unlikely to play a role in salivary gland neoplasia. Rare human papillomavirus positive cases should be carefully evaluated to exclude the possibility of a metastatic lesion.

APA, Harvard, Vancouver, ISO, and other styles

25

West, John D., Katrine M. West, and R. John Aitken. "Detection of Y-bearing spermatozoa by DNA-DNA in situ hybridisation." Molecular Reproduction and Development 1, no. 3 (1989): 201–7. http://dx.doi.org/10.1002/mrd.1080010308.

Full text

APA, Harvard, Vancouver, ISO, and other styles

26

Abu Zaanona, Mohammed Isaac, and Priyank Patel. "Plasma cell leukaemia with t(11;14) not responsive to venetoclax." BMJ Case Reports 14, no. 1 (January 2021): e238641. http://dx.doi.org/10.1136/bcr-2020-238641.

Full text

Abstract:

A 70-year-old man with medical history of IgG kappa multiple myeloma, initially diagnosed in 2017, underwent induction therapy with carfilzomib, lenalidomide and dexamethasone followed by autologous haematopoietic stem cell transplantation. Nine months following transplant, disease relapsed in the form of plasma cell leukaemia. Fluorescent in situ hybridisation of malignant plasma cells revealed t(11;14). A combination therapy including venetoclax was used based on efficacy data for Bcl-2 inhibitor venetoclax from available early-phase clinical trials in patients with relapsed multiple myeloma with t(11;14) and other published case studies. Unfortunately, the disease was primary refractory, and after further ineffective therapies, the patient did not have a successful outcome.

APA, Harvard, Vancouver, ISO, and other styles

27

Diabasana, Zania, Jeanne-Marie Perotin, Randa Belgacemi, Julien Ancel, Pauline Mulette, Claire Launois, Gonzague Delepine, et al. "Chr15q25 Genetic Variant rs16969968 Alters Cell Differentiation in Respiratory Epithelia." International Journal of Molecular Sciences 22, no. 13 (June 22, 2021): 6657. http://dx.doi.org/10.3390/ijms22136657.

Full text

Abstract:

The gene cluster region, CHRNA3/CHRNA5/CHRNB4, encoding for nicotinic acetylcholine receptor (nAChR) subunits, contains several genetic variants linked to nicotine addiction and brain disorders. The CHRNA5 single-nucleotide polymorphism (SNP) rs16969968 is strongly associated with nicotine dependence and lung diseases. Using immunostaining studies on tissue sections and air-liquid interface airway epithelial cell cultures, in situ hybridisation, transcriptomic and cytokines detection, we analysed rs16969968 contribution to respiratory airway epithelial remodelling and modulation of inflammation. We provide cellular and molecular analyses which support the genetic association of this polymorphism with impaired ciliogenesis and the altered production of inflammatory mediators. This suggests its role in lung disease development.

APA, Harvard, Vancouver, ISO, and other styles

28

Ma, Xiao-Hua, Nan Liu, Jing-Li Lu, Jie Zhao, and Xiao-Jian Zhang. "Design, Synthesis and Antiproliferative Activity of Novel Phenothiazine-1,2,3-Triazole Analogues." Journal of Chemical Research 41, no. 12 (December 2017): 696–98. http://dx.doi.org/10.3184/174751917x15122516000140.

Full text

Abstract:

A series of seven novel 1-aryl-1,2,3-triazole derivatives bearing a 4-(phenothiazin-10-ylmethyl) moiety were designed using a molecular hybridisation approach and synthesised by alkyne/azide click chemistry. Most of the synthesised compounds exhibited moderate to good antiproliferative activity (IC50 values: 0.5 to 6.7 μM) against stomach, oesophagus, prostate, breast and liver cancer cell lines, but a compound containing a 4-chlorophenyl moiety showed better activity against all cell lines than 5-fluorouracil.

APA, Harvard, Vancouver, ISO, and other styles

29

Naomi, Ruth, Juthamas Ratanavaraporn, and Mh Busra Fauzi. "Comprehensive Review of Hybrid Collagen and Silk Fibroin for Cutaneous Wound Healing." Materials 13, no. 14 (July 10, 2020): 3097. http://dx.doi.org/10.3390/ma13143097.

Full text

Abstract:

The use of hybridisation strategy in biomaterials technology provides a powerful synergistic effect as a functional matrix. Silk fibroin (SF) has been widely used for drug delivery, and collagen (Col) resembles the extracellular matrix (ECM). This systematic review was performed to scrutinise the outcome of hybrid Col and SF for cutaneous wound healing. This paper reviewed the progress of related research based on in vitro and in vivo studies and the influence of the physicochemical properties of the hybrid in wound healing. The results indicated the positive outcome of hybridising Col and SF for cutaneous wound healing. The hybridisation of these biomaterials exhibits an excellent moisturising property, perfectly interconnected structure, excellent water absorption and retention capacity, an acceptable range of biodegradability, and synergistic effects in cell viability. The in vitro and in vivo studies clearly showed a promising outcome in the acceleration of cutaneous wound healing using an SF and Col hybrid scaffold. The review of this study can be used to design an appropriate hybrid scaffold for cutaneous wound healing. Therefore, this systematic review recapitulated that the hybridisation of Col and SF promoted rapid cutaneous healing through immediate wound closure and reepithelisation, with no sign of adverse events. This paper concludes on the need for further investigations of the hybrid SF and Col in the future to ensure that the hybrid biomaterials are well-suited for human skin.

APA, Harvard, Vancouver, ISO, and other styles

30

Vanmontfort, D., AE Fidler, DA Heath, SB Lawrence, DJ Tisdall, PJ Greenwood, and KP McNatty. "cDNA sequence analysis, gene expression and protein localisation of the inhibin alpha-subunit of Australian brushtail possum (Trichosurus vulpecula)." Journal of Molecular Endocrinology 21, no. 2 (October 1, 1998): 141–52. http://dx.doi.org/10.1677/jme.0.0210141.

Full text

Abstract:

An inhibin alpha-subunit cDNA sequence from the Australian brushtail possum (Trichosurus vulpecula) has been identified and analysed. The cDNA includes an open reading frame encoding a predicted precursor protein of 361 amino acids. The predicted protein sequence includes four possible proteolytic cleavage sites, 12 evolutionarily conserved cysteine residues and three potential N-linked glycosylation sites. The mature alpha-subunit is the carboxyl terminal fragment (alphaC) consisting of 131 amino acids. The full-length precursor protein shows a mean identity with eutherian homologues of 69.8%. The homology is not evenly distributed, with the putative alphaC fragment showing the highest level (79.7%). Using Northern hybridisation, an alpha-subunit transcript of approximately 1.6 kb was detected in adult possum ovary. Using in situ hybridisation and immunocytochemistry, inhibin alpha-subunit was localised exclusively to the granulosa cell layers of follicles. Hybridisation and immunostaining for the inhibin alpha-subunit were first observed in granulosa cells of primary follicles and the expression continued throughout all stages of follicular growth. Inhibin alpha-subunit mRNA and protein were also detected in cells of the corpus luteum. In summary, results indicate considerable conservation of the structure and possible function of the inhibin alpha-subunit protein since the divergence of the marsupial and eutherian mammalian lineages. The expression data suggest that, in the adult possum, inhibin may have a role in ovarian follicular growth from the primary stage of development.

APA, Harvard, Vancouver, ISO, and other styles

31

Barua, S., A. Macedo, D. S. Kolb, K. E. Wynne-Edwards, and C. Klein. "Milk-fat globule epidermal growth factor 8 (MFGE8) is expressed at the embryo– and fetal–maternal interface in equine pregnancy." Reproduction, Fertility and Development 30, no. 4 (2018): 585. http://dx.doi.org/10.1071/rd17094.

Full text

Abstract:

Milk-fat globule epidermal growth factor (EGF) 8 protein (MFGE8), also known as lactadherin, promotes cell adhesion in an Arg-Gly-Asp (RGD)-dependent modus via integrins. In the present study, the expression of MFGE8 was examined in equine endometrium during oestrus and at Days 12 and 16 after ovulation in pregnant and non-pregnant mares and in mares during the 5th month of gestation. Results demonstrated that MFGE8 is expressed at the embryo– and fetal–maternal interface in equine pregnancy. In non-pregnant endometrium its expression was upregulated by oestrogen, a finding that was confirmed using endometrial explant culture. MFGE8 was expressed at similar levels by conceptuses collected 13 and 14 days after ovulation and by allantochorion sampled during the 5th month of gestation. Pericytes of endometrial blood vessels displayed strong MFGE8 expression upon in situ hybridisation. During the 5th month of gestation, the fetal side of the allantochorionic villi in particular displayed pronounced staining upon in situ hybridisation, confirming that MFGE8 expression is not restricted to early pregnancy but persists and is present at the fetal–maternal interface. Potential roles of MFGE8 in equine pregnancy include mediating cell–cell adhesion, promotion of angiogenesis and placental transfer of fatty acids.

APA, Harvard, Vancouver, ISO, and other styles

32

Patterson, Bruce K., Mary Ann Czerniewski, John Pottage, Michelle Agnoli, Harold Kessler, and Alan Landay. "Monitoring HIV-1 treatment in immune-cell subsets with ultrasensitive fluorescence-in-situ hybridisation." Lancet 353, no. 9148 (January 1999): 211–12. http://dx.doi.org/10.1016/s0140-6736(05)77222-6.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Kozubek, M., M. Skalnı́ková, Pe Matula, E. Bártová, J. Rauch, F. Neuhaus, H. Eipel, and M. Hausmann. "Automated microaxial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation." Micron 33, no. 7-8 (January 2002): 655–65. http://dx.doi.org/10.1016/s0968-4328(02)00023-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Dhanda, J., R. Shaw, B. Lloyd, D. R. Sibson, J. A. Woolgar, and J. M. Risk. "14 Determining molecular pathways using comparative genomic hybridisation in metastatic oral squamous cell carcinoma." British Journal of Oral and Maxillofacial Surgery 48 (May 2010): S4. http://dx.doi.org/10.1016/s0266-4356(10)60015-0.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Berrieman, H. K., J. N. E. Ashman, M. E. Cowen, J. Greenman, M. J. Lind, and L. Cawkwell. "Chromosomal analysis of non-small-cell lung cancer by multicolour fluorescent in situ hybridisation." British Journal of Cancer 90, no. 4 (February 2004): 900–905. http://dx.doi.org/10.1038/sj.bjc.6601569.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

McDonald, Sarah L., David A. J. Stevenson, Susan E. Moir, Andrew W. Hutcheon, Neva E. Haites, Steven D. Heys, and Andrew C. Schofield. "Genomic changes identified by comparative genomic hybridisation in docetaxel-resistant breast cancer cell lines." European Journal of Cancer 41, no. 7 (May 2005): 1086–94. http://dx.doi.org/10.1016/j.ejca.2005.01.018.

Full text

APA, Harvard, Vancouver, ISO, and other styles

37

Medlin, Linda K., and Sabine Strieben. "Erratum to: Refining cryptophyte identification: matching cell fixation methods to FISH hybridisation of cryptomonads." Journal of Applied Phycology 22, no. 6 (October 15, 2010): 815. http://dx.doi.org/10.1007/s10811-010-9613-8.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Shaw, Pang-Chui, Angela F. Davies, Kwok-Fai Lau, Merce Garcia-Barcelo, Mary MY Waye, Simon Lovestone, Christopher CJ Miller, and Brian H. Anderton. "Isolation and chromosomal mapping of human glycogen synthase kinase-3 α and -3β encoding genes." Genome 41, no. 5 (October 1, 1998): 720–27. http://dx.doi.org/10.1139/g98-073.

Full text

Abstract:

Glycogen synthase kinase-3 (GSK-3) is a serine-threonine kinase that exists as two isoforms, α and β, encoded by separate genes. Phosphorylation targets include a variety of cytoplasmic and nuclear proteins. Recent studies found that neurofilaments, amyloid precursor protein, and tau proteins are substrates of GSK-3 and that aberrant phosphorylation of these proteins is implicated in pathologies of the nervous system. To analyse the organisation of these two genes, a YAC library was screened by polymerase chain reaction, using primers specific for human GSK-3α and GSK-3β cDNA. Two clones, 220 and 285 kb in size, containing the complete GSK-3α coding sequence, and two clones, 365 and 285 kb in size, containing the 5 coding sequence of GSK-3β, were isolated. By somatic cell hybrid panel DNA amplification and radiation hybrid mapping, GSK-3α was found to be located at 19q13.2. On the other hand, by somatic cell hybrid panel DNA amplification and fluorescence in situ hybridisation using the 285-kb YAC clone, GSK-3β was mapped to 3q13.3.Key words: Alzheimer's disease, fluorescence in situ hybridisation, glycogen synthase kinase, neurodegeneration, radiation hybrid mapping, yeast artificial chromosome.

APA, Harvard, Vancouver, ISO, and other styles

39

Mohd Ramli, Siti Sarah, Salina Husain, and Yin Ping Wong. "Extranodal NK/T cell lymphoma, nasal type: a rare diagnosis with common nasal presentation." BMJ Case Reports 14, no. 6 (June 2021): e236436. http://dx.doi.org/10.1136/bcr-2020-236436.

Full text

Abstract:

A 39-year-old man presented with bilateral nasal obstruction for 4 months and associated with hyposmia and foul-smelling nasal discharge. Nasal endoscopy showed irregular mucosa of the nasal cavity with easily bleeding. Nasal biopsy reported as extranodal Natural Killer/T cell lymphoma, nasal type. In-situ hybridisation for Epstein-Barr encoding region was positive. He was treated with six cycles of gemcitabine, oxaliplatin and L-asparaginase and peripheral blood stem cell transplant. After the treatment, he was asymptomatic until 9 months where he had splenic abscess and undergone splenectomy. He was asymptomatic of the disease for 2 years.

APA, Harvard, Vancouver, ISO, and other styles

40

Finn, Stephen P., Paul Smyth, Esther O’Regan, Susanne Cahill, Richard Flavin, John O’Leary, and Orla Sheils. "Array comparative genomic hybridisation analysis of gamma-irradiated human thyrocytes." Virchows Archiv 445, no. 4 (July 17, 2004): 396–404. http://dx.doi.org/10.1007/s00428-004-1070-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Wilkens, Ludwig, Heidrun Gerr, Dorothea Gadzicki, Hans Kreipe, and Brigitte Schlegelberger. "Standardised fluorescence in situ hybridisation in cytological and histological specimens." Virchows Archiv 447, no. 3 (June 10, 2005): 586–92. http://dx.doi.org/10.1007/s00428-005-1211-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Cubie, Heather A., Pamela J. Molyneaux, Moira J. Shearman, J. Gryzbowski, and T. Brown. "Dot-blot hybridisation assay for detection of parvovirus B19 infections using synthetic oligonucleotides." Molecular and Cellular Probes 9, no. 1 (February 1995): 59–65. http://dx.doi.org/10.1016/s0890-8508(95)91037-9.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

McClive, P. J., T. M. Hurley, M. A. Sarraj, J. A. van den Bergen, and A. H. Sinclair. "Subtractive hybridisation screen identifies sexually dimorphic gene expression in the embryonic mouse gonad." genesis 37, no. 2 (October 2003): 84–90. http://dx.doi.org/10.1002/gene.10231.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Setiadi, Audi Francesca, and Yuri Sheikine. "CD138-negative plasma cell myeloma: a diagnostic challenge and a unique entity." BMJ Case Reports 12, no. 11 (November 2019): e232233. http://dx.doi.org/10.1136/bcr-2019-232233.

Full text

Abstract:

Plasma cell neoplasms may exhibit variations in morphology and immunophenotype, which can mimic mature B-cell lymphoproliferative disorders and pose diagnostic challenges. This case illustrates a rare entity of plasma cell myeloma, where the entire plasma cell population exhibited lymphoid morphology, negativity for CD138, positivity for CD20 and cyclin D1, and positive fluorescence in situ hybridisation for t(11;14) and del(17 p), mimicking a mature B-cell lymphoproliferative disorder, in particular mantle cell lymphoma. In this case, a careful analysis of flow cytometry gating strategies and use of other ancillary tests were keys for correct diagnosis. In addition to the diagnostic implications due to its rarity, CD138-negative plasma cell myeloma may represent a unique entity, which is associated with ‘stem cell’-like clonogenic properties, more aggressive clinical behaviour and resistance to chemotherapy.

APA, Harvard, Vancouver, ISO, and other styles

45

Chang, K. C., G. Gerlach, K. Fernandes, J. Lida, and G. Goldspink. "The influenza resistance murine Mx1 gene is constitutively expressed in the epithelia of the gastrointestinal, respiratory and uterine tracts." Journal of Cell Science 97, no. 3 (November 1, 1990): 497–502. http://dx.doi.org/10.1242/jcs.97.3.497.

Full text

Abstract:

The murine Mx1 gene confers specific resistance against influenza in the inbred A2G mice and in vitro have been shown to be inducible with type I but not type II interferons. Contrary to expectation, we found by in situ hybridisation widespread Mx1 expression along the epithelia of the gastrointestinal, uterine and respiratory tracts in uninduced A2G mice. Several lines of evidence, including further enhancement of Mx1 expression during organ culture and gnotobiotic mice analyses, indicated that this apparent constitutive epithelial Mx1 expression was a locally induced response to stimuli present in the respective lumina. This phenomenon may be a feature found in other interferon-inducible and interferon genes.

APA, Harvard, Vancouver, ISO, and other styles

46

Burger, Maximilian, Stefan Denzinger, Thomas Filbeck, Arndt Hartmann, Wolfgang Rößler, and Christina Hammerschmied. "A Metachronous, Atypical, Multifocal Renal Oncocytoma with a Concomitant Renal Cell Carcinoma of the Contralateral Side and Bilateral Multifocal Oncocytomas: Two Case Reports and Review of Literature." Scientific World JOURNAL 5 (2005): 545–49. http://dx.doi.org/10.1100/tsw.2005.73.

Full text

Abstract:

We present one case of a metachronous, atypical, multifocal renal oncocytoma with a concomitant chromophobe renal cell carcinoma (RCC) of the contralateral side and one case of bilateral and multifocal oncocytomas. Oncocytomas are benign renal tumours that rarely appear bilateral or multifocal or with coexisting RCC. A common pathogenic denominator of oncoytomas and RCC is being discussed. The first case was a 63 years old patient presenting with a history of nephrectomy for a pT1 G1 pN0 R0 papillary RCC 4 years prior to presentation, showed two tumours of a singular kidney. Upon nephron-sparing surgery one typical and one atypical oncocytoma with an invasion of the perinephric fat were found. Comparative genomic hybridisation was performed. Both tumours revealed genetic alterations with loss of genetic material on chromosome 1p. The second case was a 62 years old patient presenting with multifocal and bilateral renal tumours of undeclared dignity upon imaging. During open exploration all tumours could be removed by nephron-sparing surgery and were identified as oncocytomas. Again comparative genomic hybridisation was performed. All 4 tumours revealed genetic alterations with loss of genetic material on chromosome 1p, one of the tumours an additional loss of chromosome 10.

APA, Harvard, Vancouver, ISO, and other styles

47

Arun, C., M. DeCatris, D. M. Hemingway, N. J. M. London, and K. J. O'Byrne. "Endothelin-1 is a Novel Prognostic Factor in Non-Small Cell Lung Cancer." International Journal of Biological Markers 19, no. 4 (October 2004): 262–67. http://dx.doi.org/10.1177/172460080401900402.

Full text

Abstract:

Endothelin-1 (ET-1) is a potent vasoactive peptide and a hypoxia-inducible angiogenic growth factor associated with the development and growth of solid tumours. This study evaluated the expression of big endothelin-1 (big ET-1), a stable precursor of ET-1, and ET-1 in non-small cell lung cancer (NSCLC). Big ET-1 expression was evaluated in paraffin-embedded tissue sections from 10 NSCLC tumours using immunohistochemistry and in situ hybridisation. The production of big ET-1 and ET-1 was studied in six established NSCLC cell lines. The plasma concentrations of big ET-1 were measured in 30 patients with proven NSCLC prior to chemotherapy by means of a sandwich enzyme-linked immunoassay and compared to levels in 20 normal controls. Big ET-1 immunostaining was detected in the cancer cells of all tumours studied. Using in situ hybridisation, tumour cell big ET-1 mRNA expression was demonstrated in all samples. All six NSCLC cell lines expressed ET-1, with big ET-1 being detected in three. The median big ET-1 plasma level in patients with NSCLC was 5.4 pg/mL (range 0–22.7 pg/mL) and was significantly elevated compared to median big ET-1 plasma levels in controls, 2.1 pg/mL (1.2–13.4 pg/mL) (p=0.0001). Furthermore, patients with plasma big ET-1 levels above the normal range (upper tertile) had a worse outcome (p=0.01). In conclusion, big ET-1/ET-1 is expressed by resected NSCLC specimens and tumour cell lines. Plasma big ET-1 levels are elevated in NSCLC patients compared to controls with levels >7.8 pg/mL being associated with a worse outcome. The development of selective ET-1 antagonists such as Atrasentan indicates that ET-1 may be a therapeutic target in NSCLC.

APA, Harvard, Vancouver, ISO, and other styles

48

Sekirina, G. G., N. A. Bogoliubova, N. V. Antonova, and A. P. Dyban. "The behaviour of mitochondria and cell integration during somatic hybridisation of sister blastomeres of the 2-cell mouse embryo." Zygote 5, no. 2 (May 1997): 97–103. http://dx.doi.org/10.1017/s0967199400003762.

Full text

Abstract:

SummaryThe capacity of sister blastomeres of mouse embryos for induced fusion changed during the 2-cell stage. It was at low level (24%) at the early 2-cell stage, increased and reached 98.5% at the middle 2-cell stage and fell sharply to 31% at the late 2-cell stage. At the time corresponding to the G2/Mphase of the cell cycle the blastomeres fused in only 8% of cases. Vital staining of 2-cell embryos by rhodamine 123 showed that the mitochondria were dispersed throughout the cytoplasm with a ringlike (around the nucleus) or spot-like (over the metaphase plate) concentration in the centre of each blastomere. At the periphery of blastomeres the mitochondrial content was low. The behaviour of the mitochondria reflected the subsequent events of structural and functional integration of the sister blastomeres under induced fusion: a discernible boundary between partners during 30 min after electrofusion or 1 h after fusion with polyethylene glycol; movement of the two ‘rings’ to the centre of the blastomere fusion products (BFP) to form one large bright ‘spot’ over the common metaphase plate; mitochondria outlining the shape of the spindle and connection between sister blastomeres until completion of the first mitosis of BFP The data obtained suggest that fusion of the blastomeres does not lead to extensive changes in the hybrid cytoplasm and integration of nuclear material is taking place only at metaphase stage Cytogenetic examination of BFP at the 2-cell stage confirmed reconstruction of the tetraploid embryos and found that sister blastomeres of such embryos could asynchronously enter the next cleavage division similarly to normal diploid 2-cell embryos.

APA, Harvard, Vancouver, ISO, and other styles

49

Li, Na, Nan Liu, Shu Tang, Duo-Lu Li, and Xiao-Jian Zhang. "Synthesis and Antiproliferative Activity of Novel 1,2,3-Triazole-Sulfonamide Hybrids." Journal of Chemical Research 42, no. 1 (January 2018): 50–53. http://dx.doi.org/10.3184/174751918x15161933697853.

Full text

Abstract:

Nine novel 1-(4′-sulfamoylphenyl)-1,2,3-triazole derivatives bearing an N-heterocycle moiety were designed using a molecular hybridisation approach and synthesised by alkyne/azide click chemistry. Most of the synthesised compounds exhibited good to moderate antiproliferative activity (IC50 values 3.7 to 77.1 μM) against stomach, oesophagus and prostate cancer cell lines, but a compound containing an S-(2-pyridyl)thiomethyl moiety showed 10-fold greater activity against the stomach cell line than 5-fluorouracil. These results demonstrate that N-heterocycle-1,2,3-triazolylsulfonamides could be promising lead compounds to develop new antitumour drugs.

APA, Harvard, Vancouver, ISO, and other styles

50

Morgan, Jess A. T., Michael Macbeth, Damien Broderick, Paul Whatmore, Raewyn Street, David J. Welch, and Jennifer R. Ovenden. "Hybridisation, paternal leakage and mitochondrial DNA linearization in three anomalous fish (Scombridae)." Mitochondrion 13, no. 6 (November 2013): 852–61. http://dx.doi.org/10.1016/j.mito.2013.06.002.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!