Academic literature on the topic 'Cell hybridisation'
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Journal articles on the topic "Cell hybridisation"
Harrison, R. H., H. C. Kuo, P. N. Scriven, A. H. Handyside, and C. Mackie Ogilvie. "Lack of cell cycle checkpoints in human cleavage stage embryos revealed by a clonal pattern of chromosomal mosaicism analysed by sequential multicolour FISH." Zygote 8, no. 3 (August 2000): 217–24. http://dx.doi.org/10.1017/s0967199400001015.
Full textToebe, Kerstin. "Whole cell hybridisation for monitoring harmful marine microalgae." Environmental Science and Pollution Research 20, no. 10 (July 9, 2013): 6816–23. http://dx.doi.org/10.1007/s11356-012-1416-9.
Full textHowroyd, Simon, and Rui Chen. "Powerpath controller for fuel cell & battery hybridisation." International Journal of Hydrogen Energy 41, no. 7 (February 2016): 4229–38. http://dx.doi.org/10.1016/j.ijhydene.2016.01.038.
Full textPartridge, Wu, and Bucknall. "Investigation on the Impact of Degree of Hybridisation for a Fuel Cell Supercapacitor Hybrid Bus with a Fuel Cell Variation Strategy." Vehicles 2, no. 1 (December 19, 2019): 1–17. http://dx.doi.org/10.3390/vehicles2010001.
Full textBickmore, W. A., and A. D. Carothers. "Factors affecting the timing and imprinting of replication on a mammalian chromosome." Journal of Cell Science 108, no. 8 (August 1, 1995): 2801–9. http://dx.doi.org/10.1242/jcs.108.8.2801.
Full textTholouli, Eleni, Dolores Di Vizio, Fionnuala O’Connell, Massimo Loda, David Twomey, Todd Golub, Richard Levenson, Judith A. Hoyland, John A. L. Yin, and Richard Byers. "Quantum Dot Based Duplex In Situ Hybridisation for Gene Expression Profiling." Blood 106, no. 11 (November 16, 2005): 3265. http://dx.doi.org/10.1182/blood.v106.11.3265.3265.
Full textMCFADDEN, G. "In situ hybridisation in plants: From macroscopic to ultrastructural resolution." Cell Biology International Reports 13, no. 1 (January 1989): 3–21. http://dx.doi.org/10.1016/s0309-1651(89)80004-9.
Full textHu, Eddie, James Thompson, Sandra Horning, Martha Trela, James Lowder, Ronald Levy, and Jeffrey Sklar. "DETECTION OF B-CELL LYMPHOMA IN PERIPHERAL BLOOD BY DNA HYBRIDISATION." Lancet 326, no. 8464 (November 1985): 1092–95. http://dx.doi.org/10.1016/s0140-6736(85)90686-5.
Full textMarchio, A. "Chromosomal abnormalities in liver cell dysplasia detected by comparative genomic hybridisation." Molecular Pathology 54, no. 4 (August 1, 2001): 270–74. http://dx.doi.org/10.1136/mp.54.4.270.
Full textGavins, Georgina C., Katharina Gröger, Marc Reimann, Michael D. Bartoschek, Sebastian Bultmann, and Oliver Seitz. "Orthogonal coiled coils enable rapid covalent labelling of two distinct membrane proteins with peptide nucleic acid barcodes." RSC Chemical Biology 2, no. 4 (2021): 1291–95. http://dx.doi.org/10.1039/d1cb00126d.
Full textDissertations / Theses on the topic "Cell hybridisation"
Harrison, J. G. "Studies on the cell uptake and hybridisation properties of oligonucleotide derivatives." Thesis, University of Cambridge, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.603788.
Full textMcCutchan, Jennifer Susan. "Transferring ascochyta blight resistance from Lathyrus sp. into field pea (Pisum sativum L.) via protoplast fusion (somatic hybridisation) /." Connect to thesis, 2001. http://eprints.unimelb.edu.au/archive/00000696.
Full textJiang, Sheng. "Application of nested PCR, whole genome amplification and comparative genomic hybridisation for single cell genetic analysis." Thesis, University of Glasgow, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366140.
Full textWilkins, Bridget Sally. "Cell-stroma interactions in haemopoiesis studied by immunocytochemistry and in situ hybridisation in long-term cultures and trephine biopsies of human bone marrow." Thesis, University of Southampton, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.242221.
Full textKarlsson, Christina. "Biomarkers in non-small cell lung carcinoma : methodological aspects and influence of gender, histology and smoking habits on estrogen receptor and epidermal growth factor family receptor signalling." Doctoral thesis, Örebro universitet, Hälsoakademin, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-19725.
Full textBrandt, Stephan Peter. "Zelltyp-spezifische Mikroanalyse von Arabidopsis thaliana-Blättern." Phd thesis, Universität Potsdam, 2001. http://opus.kobv.de/ubp/volltexte/2005/40/.
Full textFür die Erstellung von Gewebe-spezifischen Expressionsprofilen war es notwendig, die in vereinigten Zellproben enthaltene mRNA zunächst zu amplifizieren, um eine ausreichende Menge für Arrayhybridisierungen zu erhalten. Vor der Vermehrung wurde die mRNA revers transkribiert. Es wurden daran anschließend verschiedene Amplifikationsstrategien getestet: Die neben Tailing, Adapterligation und anderen PCR-basierenden Protokollen getestete Arbitrary-PCR hat sich in dieser Arbeit als einfache und einzige Methode herausgestellt, die mit so geringen cDNA-Mengen reproduzierbar arbeitet. Durch Gewebe-spezifische Array-hybridisierungen mit der so amplifizierten RNA konnten schon bekannte Expressionsmuster verschiedener Gene, vornehmlich solcher, die an der Photosynthese beteiligt sind, beobachtet werden. Es wurden aber auch eine ganze Reihe neuer offensichtlich Gewebe-spezifisch exprimierter Gene gefunden. Exemplarisch für die differentiell exprimierten Gene konnte das durch Arrayhybridisierungen gefundene Expressionsmuster der kleinen Untereinheit von Rubisco verifiziert werden. Hierzu wurden Methoden zum Gewebe-spezifischen Northernblot sowie semiquantitativer und Echtzeit-Einzelzell-RT-PCR entwickelt.
Im zweiten Teil der Arbeit wurden Methoden zur Analyse von Metaboliten einschließlich anorganischer Ionen verwendet. Es stellte sich heraus, daß die multiparallele Methode der Gaschromatographie-Massenspektrometrie keine geeignete Methode für die Analyse selbst vieler vereinigter Zellinhalte ist. Daher wurde auf Kapillarelektrophorese zurückgegriffen. Eine Methode, die mit sehr kleinen Probenvolumina auskommt, eine hohe Trennung erzielt und zudem extrem geringe Detektionslimits besitzt. Die Analyse von Kohlenhydraten und Anionen erfordert eine weitere Optimierung. Über UV-Detektion konnte die K+-Konzentration in verschiedenen Geweben von A. thaliana bestimmt werden. Sie lag in Epidermis und Mesophyll mit ca. 25 mM unterhalb der für andere Pflanzenspezies (Solanum tuberosum und Hordeum vulgare) publizierten Konzentration. Weiter konnte gezeigt werden, daß zwölf freie Aminosäuren mittels einer auf Kapillarelektrophorese basierenden Methode in vereinigten Zellproben von Cucurbita maxima identifiziert werden konnten. Die Übertragung der Methode auf A. thaliana-Proben muß jedoch weiter optimiert werden, da die Sensitivität selbst bei Laser induzierter Fluoreszenz-Detektion nicht ausreichte.
Im dritten und letzten Teil der Arbeit wurde eine Methode entwickelt, die die Analyse bekannter wie unbekannter Proteine in Gewebe-spezifischen Proben ermöglicht. Hierzu wurde zur Probennahme mittels mechanischer Mikrodissektion eine alternative Methode zur Laser Capture Microdissection verwendet, um aus eingebetteten Gewebeschnitten distinkte Bereiche herauszuschneiden und somit homogenes Gewebe anzureichern. Aus diesem konnten die Proteine extrahiert und über Polyacrylamidgelelektrophorese separariert werden. Banden konnten ausgeschnitten, tryptisch verdaut und massenspektrometrisch die Primärsequenz der Peptidfragmente bestimmt werden. So konnten als Hauptproteine im Mesophyll die große Untereinheit von Rubisco sowie ein Chlorophyll bindendes Protein gefunden werden.
Die in dieser Arbeit entwickelten und auf die Modellpflanze Arabidopsis thaliana angewandten Einzelzellanalysetechniken erlauben es in Zukunft, physiologische Prozesse besser sowohl räumlich als auch zeitlich aufzulösen. Dies wird zu einem detaillierteren Verständnis mannigfaltiger Vorgänge wie Zell-Zell-Kommunikation, Signalweiterleitung oder Pflanzen-Pathogen-Interaktionen führen.
The subject of this thesis was the analysis of single plant cells in respect to their contents of i) transcripts, ii) inorganic cations and anions, iii) metabolites like amino acids and carbohydrates as well as iv) proteins. One task was the transfer of existing methods to single cell analysis on leaf tissues of the model plant Arabisopsis thaliana L., the second one was the refinement and the development, respectively, of new protocols for the analysis of such picoliter samples. For cell type specific sampling two different complimentary methods were applied: Using micro glass capillaries specific single cell contents could be harvested from intact plants, whereas typical sample volumes were in the picoliter range. Even the sampling of inner cell types such as companion cells could be demonstrated. Using mechanical micro dissection of embedded tissue a larger amount of homogenous tissue could be collected.
Because single cell samples contain only femtogram amounts of mRNA, direct detection of transcripts is impossible. Therefore, two amplification protocols were applied to the cell samples: The first procedure makes use of specifically primed RT-PCR for amplification. Several genes derived from different plants and tissues could be detected after successful RT-PCR, including high as well as low expressed genes. The second method was developed to monitor the activity of many genes in parallel using array hybridisation with filters containing the cDNA of as many as 16.000 ESTs. For this purpose, unspecific RT-PCR as it is applied in the differential display was used to amplify different transcripts in just one reaction. However, in these tissue specific array hybridisations the expression patterns of several hundreds genes could be monitored. These included known tissue specific expression patterns (of mainly photosynthesis related genes) as well as a couple of unknown expression patterns. To verify the tissue specificity of gene activity some results were reconsidered using tissue specific northern blot hybridisations and real time RT-PCR, respectively.
Secondly, metabolites (including inorganic ions) were investigated: Because gas chromatography-mass spectrometry does not reveal the sensitivity which in necessary for the analysis of even multiple pooled single cell samples capillary electrophoresis was applied for these studies. This method has a high potential as it needs only small amounts of starting material, has uncomparable low detection limits and exhibits a high number of theoretical plates.
The analysis of inorganic anions and carbohydrates needs further optimisations. Using UV absorption-detection potassium could be detected in different cell types whereas the concentrations in mesophyll and epidermis were found around 25 mM each. These concentrations are lower than in other species as Solanum tuberosum or Hordeum vulgare. For investigations of amino acids the cell samples were derivatized to make the use of laser induced fluorescence-detection capable. In samples derived from pumpkin (Cucurbita maxima) mesophyll twelve amino acids could be detected and identified. The transfer of this method to A. thaliana derived samples exhibited no results which may be due to the low concentration of free amino acids in these plants.
Finally, a method was developed with which the existence of known and unknown proteins in tissue specific samples could be monitored. For this, mechanical micro dissection was used to: After embedding and sectioning the tissue of interest was cut out by an vibrating steel chisel to get homogenous samples. The proteins contained in these tissue pieces were extracted and separated by one dimensional SDS polyacrylamid gel electrophoresis. Several protein bands could be detected after staining with either silver or coomassie blue stain. These bands were cut out and sequenced by mass spectrometry. The large subunit of rubisco as well as one chlorophyll binding protein could be identified as the major proteins within the mesophyll.
The single cell analysis methods which were developed and applied to the model plant A. thaliana in this thesis allow a better spatial as well as temporal resolution of analysis. This will lead to a more detailed understanding of physiological processes like cell to cell communication, signalling or plant-pathogen interactions.
Muharam, Firman Alamsyah. "Overcoming problems with limiting DNA samples in forensics and clinical diagnostics using multiple displacement amplification." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16207/.
Full textBulman, S. R. "Testing the effect of in planta RNA silencing on Plasmodiophora brassicae infection." Lincoln University, 2006. http://hdl.handle.net/10182/1856.
Full textXu, Meng. "Specialised transcription factories." Thesis, University of Oxford, 2008. http://ora.ox.ac.uk/objects/uuid:a41d3243-c233-491a-916b-4e329cace434.
Full textBui, Loan Thuy. "Localisation of kallikreins in the prostate and association with prostate cancer progression." Queensland University of Technology, 2006. http://eprints.qut.edu.au/16276/.
Full textBook chapters on the topic "Cell hybridisation"
Davenport, Russell James, and Thomas Peter Curtis. "Section 7 update: Quantitative fluorescence in situ hybridisation (FISH): statistical methods for valid cell counting." In Molecular Microbial Ecology Manual, 3389–417. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-2177-0_707.
Full textRanabhatt, Hiru, and Renu Kapor. "Somatic cell hybridisation." In Plant Biotechnology, 177–89. WPI Publishing, 2017. http://dx.doi.org/10.1201/9780429505676-10.
Full textSottile, Virginie. "Detection of Stem Cell Populations Using in Situ Hybridisation." In Biomedical Engineering, Trends, Research and Technologies. InTech, 2011. http://dx.doi.org/10.5772/13519.
Full text"Isolation of Genomic DNA From Plant Tissues." In Protocols used in Molecular Biology, edited by Pallavi Singh, 1–6. BENTHAM SCIENCE PUBLISHERS, 2020. http://dx.doi.org/10.2174/9789811439315120010004.
Full textHicks, R. E., and D. A. Pascoe. "A comparison of cyanobacterial dominance within the picoplankton of the North American Great Lakes estimated by 16S rRNA-based hybridisations and direct cell counts." In The Great Lakes of the World (GLOW), 363–74. Michigan State University Press, 2001. http://dx.doi.org/10.14321/j.ctt1bqzmb5.23.
Full textConference papers on the topic "Cell hybridisation"
Winkler, Wolfgang. "Fuel Cell Hybrids, Their Thermodynamics and Sustainable Development." In ASME 2005 3rd International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2005. http://dx.doi.org/10.1115/fuelcell2005-74157.
Full textLoetsch, Daniela, Christine Pirker, Sabine Spiegl-Kreinecker, Michael Grusch, Johannes Fischer, Michael Micksche, and Walter Berger. "Abstract 5142: Detection of genomic changes in glioblastoma stem cell subpopulations by array comparative genomic hybridisation." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-5142.
Full textPunch, Jeff, Bryan Rodgers, David Newport, and Mark Davies. "Thermal Analysis of a Micro-Polymerase Chain Reaction Device." In ASME 2004 International Mechanical Engineering Congress and Exposition. ASMEDC, 2004. http://dx.doi.org/10.1115/imece2004-59161.
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