Relevant bibliographies by topics / Cell culture

Academic literature on the topic 'Cell culture'

Author: Grafiati
Published: 4 June 2021
Last updated: 1 August 2024

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Journal articles on the topic "Cell culture"

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Yoshino, T. P., U. Bickham, and C. J. Bayne. "Molluscan cells in culture: primary cell cultures and cell lines." Canadian Journal of Zoology 91, no. 6 (June 2013): 391–404. http://dx.doi.org/10.1139/cjz-2012-0258.

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In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as biomonitors for environmental contaminants, as models for gene transfer technologies, and for studies of innate immunity and neoplastic disease. Despite efforts to isolate proliferative cell lines from molluscs, the snail Biomphalaria glabrata (Say, 1818) embryonic (Bge) cell line is the only existing cell line originating from any molluscan species. Taking an organ systems approach, this review summarizes efforts to establish molluscan cell cultures and describes the varied applications of primary cell cultures in research. Because of the unique status of the Bge cell line, an account is presented of the establishment of this cell line, and of how these cells have contributed to our understanding of snail host – parasite interactions. Finally, we detail the difficulties commonly encountered in efforts to establish cell lines from molluscs and discuss how these difficulties might be overcome.
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Ylostalo, Joni H. "3D Stem Cell Culture." Cells 9, no. 10 (September 27, 2020): 2178. http://dx.doi.org/10.3390/cells9102178.

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Much interest has been directed towards stem cells, both in basic and translational research, to understand basic stem cell biology and to develop new therapies for many disorders. In general, stem cells can be cultured with relative ease, however, most common culture methods for stem cells employ 2D techniques using plastic. These cultures do not well represent the stem cell niches in the body, which are delicate microenvironments composed of not only stem cells, but also supporting stromal cells, extracellular matrix, and growth factors. Therefore, researchers and clinicians have been seeking optimal stem cell preparations for basic research and clinical applications, and these might be attainable through 3D culture of stem cells. The 3D cultures recapitulate the in vivo cell-to-cell and cell-to-matrix interactions more effectively, and the cells in 3D cultures exhibit many unique and desirable characteristics. The culture of stem cells in 3D may employ various matrices or scaffolds, in addition to the cells, to support the complex structures. The goal of this Special Issue is to bring together recent research on 3D cultures of various stem cells to increase the basic understanding of stem cells and culture techniques, and also highlight stem cell preparations for possible novel therapeutic applications.
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Orlidge, A., and P. A. D'Amore. "Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells." Journal of Cell Biology 105, no. 3 (September 1, 1987): 1455–62. http://dx.doi.org/10.1083/jcb.105.3.1455.

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Morphological studies of developing capillaries and observations of alterations in capillaries associated with pathologic neovascularization indicate that pericytes may act as suppressors of endothelial cell (EC) growth. We have developed systems that enable us to investigate this possibility in vitro. Two models were used: a co-culture system that allowed direct contact between pericytes and ECs and a co-culture system that prevented physical contact but allowed diffusion of soluble factors. For these studies, co-cultures were established between bovine capillary ECs and the following growth-arrested cells (hereafter referred to as modulating cells): pericytes, smooth muscle cells (SMCs), fibroblasts, epithelial cells, and 3T3 cells. The modulating cell type was growth arrested by treatment with mitomycin C before co-culture with ECs. In experiments where cells were co-cultured directly, the effect of co-culture on EC growth was determined by comparing the mean number of cells in the co-cultures to the mean for each cell type (EC and modulating cell) cultured separately. Since pericytes and other modulating cells were growth arrested, any cell number change in co-cultures was due to EC growth. In the co-cultures, pericytes inhibited all EC proliferation throughout the 14-d time course; similar levels of EC inhibition were observed in SMC-EC co-cultures. Co-culture of ECs with fibroblasts, epithelial cells, and 3T3 cells significantly stimulated EC growth over the same time course (30-192% as compared to EC cultured alone). To determine if cell contact was required for inhibition, cells were co-cultured using Millicell chambers (Millipore Corp., Bedford, MA), which separated the cell types by 1-2 mm but allowed the exchange of diffusible materials. There was no inhibition of EC proliferation by pericytes or SMCs in this co-culture system. The influence of the cell ratios on observed inhibition was assessed by co-culturing the cells at EC/pericyte ratios of 1:1, 2:1, 5:1, 10:1, and 20:1. Comparable levels of EC inhibition were observed at ratios from 1:1 to 10:1. When the cells were co-cultured at a ratio of 20 ECs to 1 pericyte, inhibition of EC growth at 3 d was similar to that observed at other ratios. However, at higher ratios, the inhibition diminished so that by the end of the time course the co-cultured ECs were growing at the same rate as the controls. These results suggest that pericytes and SMCs can modulate EC growth by a mechanism that requires contact or proximity. We postulate that similar interactions may operate to modulate vascular growth in vivo.
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Lindee, M. S. "CELL BIOLOGY: The Culture of Cell Culture." Science 316, no. 5831 (June 15, 2007): 1568–69. http://dx.doi.org/10.1126/science.1142574.

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Bauer, Magdalena, Magdalena Metzger, Marvin Corea, Barbara Schädl, Johannes Grillari, and Peter Dungel. "Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture." Life 12, no. 8 (August 10, 2022): 1216. http://dx.doi.org/10.3390/life12081216.

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In skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of ‘3Rs’, new approaches such as 3D skin equivalents (SE) are needed to close the in vitro/in vivo gap. Cell culture inserts to culture SE are commercially available, however, these inserts are expensive and of limited versatility regarding experimental settings. This study aimed to design novel cell culture inserts fabricated on commercially available 3D printers for the generation of full-thickness SE. A computer-aided design model was realized by extrusion-based 3D printing of polylactic acid filaments (PLA). Improvements in the design of the inserts for easier and more efficient handling were confirmed in cell culture experiments. Cytotoxic effects of the final product were excluded by testing the inserts in accordance with ISO-norm procedures. The final versions of the inserts were tested to generate skin-like 3D scaffolds cultured at an air–liquid interface. Stratification of the epidermal component was demonstrated by histological analyses. In conclusion, here we demonstrate a fast and cost-effective method for 3D-printed inserts suitable for the generation of 3D cell cultures. The system can be set-up with common 3D printers and allows high flexibility for generating customer-tailored cell culture plastics.
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Basse, Per, Ryan Fan, Lisa Bailey, Jay Wynn, and Michael Lotze. "Nk cells induce tumor cell resistance to Nk cell cytolysis (TUM2P.918)." Journal of Immunology 192, no. 1_Supplement (May 1, 2014): 71.42. http://dx.doi.org/10.4049/jimmunol.192.supp.71.42.

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Abstract Live cell microscopy of murine NK cells co-cultured with tumor cells revealed substantial lysis of tumor cells by NK cells that diminished dramatically within the first few hours. The kinetics of conjugate formation and NK cell cycling between targets remained unchanged. When NK cells were re-isolated and placed in co-culture with fresh tumor cells, substantial lysis ensued, indicating that the reduction in killing was not due to exhaustion of the NK cells. When tumor cells were isolated from co-cultures and re-mixed with fresh NK cells, normal cycling and conjugate-formation with tumor cells was observed, but this rarely resulted in cytolysis. Tumor cells lost plastic-adherence and underwent replicative senescence. When removed from co-culture, most of the still viable tumor cells regained their normal adherent morphology, growth rate, and sensitivity to NK-mediated lysis. Pre-incubation of tumor cells with cell-free supernatants from NK cell or NK+tumor cell cultures reduced the killing by 60-90% compared to that of non-preincubated tumor cells. The changes in MHC expression correlated poorly with the observed changes in tumor cell resistance to NK cell-mediated cytotoxicity. Pre-incubation of tumor cells with NK cells from IFNγ-KO donors or supernatants of IFNγ-deficient NK cells did not cause MHC upregulation, but induced almost complete resistance in tumor cells to NK cell-mediated cytolysis. Both IFNγ and MHC upregulation are dispensable for the observed protection.
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Sandstrom, CE, JG Bender, ET Papoutsakis, and WM Miller. "Effects of CD34+ cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells." Blood 86, no. 3 (August 1, 1995): 958–70. http://dx.doi.org/10.1182/blood.v86.3.958.958.

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Abstract Ex vivo expansion of peripheral blood mononuclear cells (MNCs), cultured both directly and after selection for CD34+ cells, was compared in static and continuously perfused cultures containing interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor (G- CSF), and stem cell factor (SCF). Cultures inoculated with either MNCs or CD34+ cells produced cells that were remarkably similar after 10 days of culture, as evidence by cell morphology, expression of CD34, CD33, CD15, and CD11b, and the fractions of cells giving rise to colony- forming units granulocyte-monocyte (CFU-GM) and long-term culture- initiating cells (LTC-IC). Static and perfusion cultures gave similar average total cells and CFU-GM expansions for both MNC and CD34+ cell cultures. However, those samples that performed poorly in static culture performed at near-normal levels in perfusion. In addition, perfusion supported higher LTC-IC numbers for both MNC and CD34+ cell cultures. While total cell expansion was about ten times greater in CD34+ cell cultures (approximately 100-fold), CFU-GM expansion (approximately 20-fold) was similar for both MNC and CD34+ cell cultures. The similar distribution of cell types produced in MNC and CD34+ cell cultures allows direct comparison of total and colony- forming cell production. After 15 days in perfusion, MNC cultures produced 1.5-, 2.6-, and 2.1-fold more total cells, CFU-GM, and LTC-IC, respectively, than the same sample selected and cultured as CD34+ cells. Even if the CD34+ selection process was 100% efficient, CFU-GM production would be 1.5-fold greater for MNCs than for CD34+ cells.
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Sandstrom, CE, JG Bender, ET Papoutsakis, and WM Miller. "Effects of CD34+ cell selection and perfusion on ex vivo expansion of peripheral blood mononuclear cells." Blood 86, no. 3 (August 1, 1995): 958–70. http://dx.doi.org/10.1182/blood.v86.3.958.bloodjournal863958.

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Ex vivo expansion of peripheral blood mononuclear cells (MNCs), cultured both directly and after selection for CD34+ cells, was compared in static and continuously perfused cultures containing interleukin (IL)-3, IL-6, granulocyte colony-stimulating factor (G- CSF), and stem cell factor (SCF). Cultures inoculated with either MNCs or CD34+ cells produced cells that were remarkably similar after 10 days of culture, as evidence by cell morphology, expression of CD34, CD33, CD15, and CD11b, and the fractions of cells giving rise to colony- forming units granulocyte-monocyte (CFU-GM) and long-term culture- initiating cells (LTC-IC). Static and perfusion cultures gave similar average total cells and CFU-GM expansions for both MNC and CD34+ cell cultures. However, those samples that performed poorly in static culture performed at near-normal levels in perfusion. In addition, perfusion supported higher LTC-IC numbers for both MNC and CD34+ cell cultures. While total cell expansion was about ten times greater in CD34+ cell cultures (approximately 100-fold), CFU-GM expansion (approximately 20-fold) was similar for both MNC and CD34+ cell cultures. The similar distribution of cell types produced in MNC and CD34+ cell cultures allows direct comparison of total and colony- forming cell production. After 15 days in perfusion, MNC cultures produced 1.5-, 2.6-, and 2.1-fold more total cells, CFU-GM, and LTC-IC, respectively, than the same sample selected and cultured as CD34+ cells. Even if the CD34+ selection process was 100% efficient, CFU-GM production would be 1.5-fold greater for MNCs than for CD34+ cells.
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Kakigi, Akinobu. "Cell Culture." Equilibrium Research 67, no. 1 (2008): 1–5. http://dx.doi.org/10.3757/jser.67.1.

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Ponec, Maria, and Esther Boelsma. "Cell Culture." American Journal of Contact Dermatitis 8, no. 2 (June 1997): 100–102. http://dx.doi.org/10.1097/01634989-199706000-00021.

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Dissertations / Theses on the topic "Cell culture"

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Liu, Mengfei, and 刘梦菲. "Epithelial morphogenesis in three-dimensional cell culture system." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2014. http://hdl.handle.net/10722/208611.

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In human body, the most common structures formed by epithelial cells are hollow cysts or tubules. The key feature of the cysts and tubules is the central lumen, which is lined by epithelial cell sheets. The central lumen allows material exchange, thus it is indispensable for the proper function of the epithelial tissue. In order to understand the way that the epithelial cells form highly specialized structure, an in vitro three-dimensional (3D) culture system was established. The Caco-2 cells were embedded in reconstituted basement membrane termed matrigel, whose biochemical constitution and physical properties were similar with the in vivo environment. The Caco-2 cells in matrigel spontaneously formed spherical multi-cell cysts, which could continuously expand. The confocal imaging and reconstruction technique helped understand the cyst structure and its formation process. The cysts developed central lumen surrounded by a layer of polarized cells. The apical domain of the cells faced the lumen, while the basal domain attached to the extracellular matrix. In the mature cysts, fluid was secreted by the cells around the lumen at the apical domain, and accumulated in the central lumen. The laser burning experiment showed that the intraluminal pressure was higher than the outer environment. The intact cell sheet was required to keep the engorged morphology of the cysts. The tension of the cell layer balanced with the intraluminal pressure. To investigate the effect of pressure on cyst development, the cysts were treated with cholera toxin, which could increase intraluminal pressure through promoting apical secretion. The time-lapse images showed that under cholera toxin treatment, the expansion of the cysts was accelerated. The high intraluminal pressure led to shape change of thecells, followed by increase in cell proliferation rate. Cholera toxin itself could not promote cell growth. In the3D cultured cysts, it was the increased intraluminal pressure that directly induced the acceleration of cell proliferation. It indicated that not only biochemical signals, but also mechanical force, contributed to epithelial morphogenesis. The mechanical stimulation could be converted into biochemical signals, further affect cell behavior. In response to mechanical stimulation, the focal adhesion kinase was activated in the cells around the cyst lumen. Furthermore, the microarray analysis suggested that multiple signaling pathways were altered under intraluminal pressure stimulation, including the pathways related to cytoskeleton organization, cell cycle and cell adhesion. Taken together, comparing with the conventional two-dimensional cell culture on rigid surface, the three-dimensional culture system provided the cells a more physiological environment. The 3D culture system allows the epithelial cells to form well-organized hollow structure. It is a convenient model for investigating the process and mechanism of epithelial morphogenesis.
published_or_final_version
Biochemistry
Doctoral
Doctor of Philosophy
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Theil, Ian. "Anchorage-dependent mammalian cell culture." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56768.

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Genetically engineered anchorage-dependent human embryonic kidney (293) cells were cultured at 37$ sp circ$C on 1 mm thick sheets of a fibrous polymeric matrix having an average fibre diameter of 10.2 $ mu$m and a void fraction of 0.81 using Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 2.5 mM glutamine. Immobilization efficiencies above 70% were observed when cells were added to 100 mL spinner flasks (operating at 60 rpm) containing 70 mL of medium and two 1 x 1 cm squares of matrix (total gross area of 2 cm$ sp2$) fastened to the base of the stirrer shaft. Loadings in excess of 2.4 $ times 10 sp7$ cells per cm$ sp2$ of matrix were measured after 2 h.
The state of the cultures was followed by measuring the consumption of glucose and glutamine and the production of lactate and ammonium.
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Machado, Roque Ana Isabel. "Protein scaffolds for cell culture." Thesis, University of Newcastle Upon Tyne, 2013. http://hdl.handle.net/10443/1843.

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We report here the design, purification and structural characterization of a new protein scaffold for cell culture. Prior studies in our group revealed the structure of the bacterial protein Caf1 to be flexible protein nanofibres, up to 1.5 μm. The existing Cafl expression system was cumbersome and difficult to mutate, we have now produced a system containing the caf operon which allows for the incorporation of specific peptide motifs. The small peptide, RGDS from fibronectin was inserted into 5 different surface loops of Caf1. The Caf1 mutants were expressed and purified and a structural characterization by biophysical methods was conducted. This revealed permissive sites into which new motifs can be inserted. The characterised proteins were sterilised and used to coat 96 well plates for cell culture. In this study we used mammalian cell lines such as 3T3 fibroblasts, PC12 neuronal cells and primary osteoblasts to understand how they behave in the presence of this biomaterial, in particular the formation of focal adhesions, changes in cytoskeleton rearrangement and nuclear and cell morphology. The controlled engineering of sites within the polymer allowed us to study their implication in cell attachment, survival and proliferation. Our preliminary results have shown that cells interact poorly with the unmodified protein e.g. without any motif associated. This reveals that the polymer is inert and does not influence cell growth by itself. In contrast, the incorporation of RGDS, can invert the scenario of cell growth; promoting cell attachment, survival and proliferation. In a second stage of the project we designed a separate compatible plasmid encoding caf1 gene and used it with the previous plasmid to co-express hybrid Caf1 polymers. The long fibres can also be crosslinked with a non-toxic and non-immunogenic chemical compound – NHS-PEG. Thus a protein hydrogel composed of interchangeable folding units which can be used to incorporate different cell interacting peptide motifs. It is robust and, in the unmodified state highly protease resistant. Future studies will elucidate the versatility and potentiality for this peptide hydrogel in stem cell differentiation.
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Lee, Kevin Shao-Kwan. "Microscale controlled continuous cell culture." Thesis, Massachusetts Institute of Technology, 2011. http://hdl.handle.net/1721.1/64579.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2011.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 489-500).
Measurements of metabolic and cellular activity through substrate and product interactions are highly dependent on environmental conditions and cellular metabolic state. For such experiments to be feasible, continuous cultures are utilized to ensure consistent conditions. However, since medium must be replenished every cell doubling time, costs can be prohibitive in large reactors. An integrated microscale bioreactor with built-in fluid metering and environmental control will enable programmed experiments capable of generating reproducible data routinely. This work develops an instrument capable of supporting automated microscale continuous culture experiments. The instrument consists of a plastic-PDMS device capable of continuous flow reactions without volume drift. A novel bonding process is invented to fabricate devices with chemically stable interfaces against water, acids, and bases. We introduce a direct CNC machining and chemical bonding fabrication process for production of fluidic devices with a 1 mL working volume, high oxygen transfer rate (kLa ~ 0.025 s-1), fast mixing (2 s), accurate flow control (± 18 nL), and closed loop control over temperature, cell density, oxygen, and pH. Providing control over environmental parameters allows the system to perform different types of cell culture on a single device, such as batch, fed-batch, chemostat, and turbidostat continuous culture. Validation experiments demonstrate that cells can be grown to high optical densities (OD = 50) and production of commercially relevant chemicals such as DNA vaccines are comparable to large scale bench fermentations. Continuous cultures are also demonstrated without contamination for 3 weeks in a single device and both steady state and dynamically controlled conditions are possible, allowing observations of cell metabolic dynamics.
by Kevin Shao-Kwan Lee.
Ph.D.
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Schley, Jeremiah P. "Single Cell Culture Wells (SiCCWells)." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1406292709.

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Balagopal, Tulika C., Xiao Lucy Cheng, Jessica Gamboa, John Harper, Sheridan McPheeters, and Bryce Notheis. "A Dynamic Cell Culture System." Thesis, The University of Arizona, 2010. http://hdl.handle.net/10150/146852.

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The following includes information regarding the design, construction, and initial testing of A Dynamic Cell Culture System. This project has been undertaken in order to provide the Wu Lab with a tool that will allow a method to more accurately simulate human vasculature for research purposes by applying pulsatile shear, tensile, and normal pressures. This is accomplished by utilizing upper and lower Chambers between which reside tissue scaffolds (composed of silk and elastin) which are seeded with cells. A high flow-rate pump is used to pass cell culture media over the scaffolds in order to create shear. Solenoid valves are used in conjunction with pressure sensors to cause pressure to build up in one Chamber, causing both normal and tensile stresses in the scaffolds. At this time, the system has been fully constructed and has undergone only initial testing, the results of which showed that more work on sealing the Chamber and tube connections is necessary.
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Reiland, Joanne Elizabeth Donovan Maureen D. "Analysis of cell culture models of mammary drug transport." Iowa City : University of Iowa, 2009. http://ir.uiowa.edu/etd/316.

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Ekström, Jens-Ola. "Ljungan Virus Replication in Cell Culture." Doctoral thesis, Högskolan i Kalmar, Naturvetenskapliga institutionen, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-10.

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Ljungan virus (LV) is a recently identified picornavirus of the genus Parechovirus. LV has been isolated from voles trapped in Sweden and also in the United States. LV infected small rodents may suffer from diabetes type 1 and type 2 like symptoms, myocarditis and encephalitis. LV has been proposed as a human pathogen, with indications of causing diabetes type 1, myocarditis and intrauterine fetal deaths. In this thesis, cell culture adapted LV strains were utilised for development and adaptation of several basic methodological protocols to study the LV biology, e.g. real time PCR, highly specific antibodies and a reverse genetics system. These methods allowed detailed studies of this virus and how it interacts with the host cell. The genomic 5'-end was identified and modelling showed unique secondary structure folding of this region. The LV encodes an aphthovirus-like 2A protein with a DvExNPGP motif. This motif was found to mediate primary cleavage of the LV polyprotein in vitro and is proposed to constitute the carboxy terminus of the structural protein VP1 in LV. Rabbit polyclonal antibodies generated against recombinant structural proteins were used to verify that the LV virion is composed of the structural proteins VP0, VP1 and VP3. Cell culture studies showed that LV replicates to low titer with an absent or delayed cell lysis. LV is proposed to be able to spread by a, for picornaviruses, not previously demonstrated direct cell-to-cell transmission. All results taken together suggest a maintenance strategy of LV including low amounts of the LV genome and persistently infected hosts. Stability studies showed that the LV virion not only maintain activity in acidic and alkaline environments but also exhibit resistance to the commonly used disinfectant Virkon®.The results presented in this thesis show that LV has several unique properties, not previously observed for a picornavirus.
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Ekström, Jens-Ola. "Ljungan virus replication in cell culture /." Kalmar : University of Kalmar, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hik:diva-10.

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Jayawarna, Vineetha. "Fmoc-peptide gels for cell culture." Thesis, University of Manchester, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.489519.

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Spontaneous formation of macroscopic hydrogels from small molecule building blocks via self-assembly is a powerful tool for the preparation of novel materials with well defined properties. Peptides are particularly interesting as building blocks for these materials. Self-assembled nanowires, fibres, sheets and tubes from peptide systems have all been described.
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Books on the topic "Cell culture"

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Michael, Conn P., ed. Cell culture. San Diego: Academic Press, 1990.

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Aschner, Michael. Cell culture techniques. New York, N.Y: Humana Press, 2011.

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M, Loyola-Vargas Victor, and Vázquez-Flota Felipe, eds. Plant cell culture protocols. 2nd ed. Totowa, N.J: Humana Press, 2006.

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1931-, Pinson Arié, ed. The Heart cell in culture. Boca Raton, Fla: CRC Press, 1987.

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W, Pollard Jeffrey, and Walker John M. 1948-, eds. Animal cell culture. Clifton, N.J: Humana Press, 1990.

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Joanna, Picot, ed. Human cell culture protocols. 2nd ed. Totowa, N.J: Humana Press, 2005.

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J, Jones Christopher, ed. Epithelia: Advances in cell physiology and cell culture. Dordrecht: Kluwer Academic Publishers, 1990.

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Pollard, Jeffrey W., and John M. Walker. Animal Cell Culture. New Jersey: Humana Press, 1990. http://dx.doi.org/10.1385/0896031500.

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Langdon, Simon P. Cancer Cell Culture. New Jersey: Humana Press, 2003. http://dx.doi.org/10.1385/1592594069.

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Amini, Shohreh, and Martyn K. White, eds. Neuronal Cell Culture. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1437-2.

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Book chapters on the topic "Cell culture"

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Buxbaum, Engelbert. "Cell Culture." In Biophysical Chemistry of Proteins, 187–89. Boston, MA: Springer US, 2010. http://dx.doi.org/10.1007/978-1-4419-7251-4_17.

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Nahler, Gerhard. "cell culture." In Dictionary of Pharmaceutical Medicine, 23. Vienna: Springer Vienna, 2009. http://dx.doi.org/10.1007/978-3-211-89836-9_172.

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Messina, Antonietta, and Loredana De Bartolo. "Cell Culture." In Encyclopedia of Membranes, 336–38. Berlin, Heidelberg: Springer Berlin Heidelberg, 2016. http://dx.doi.org/10.1007/978-3-662-44324-8_1199.

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Wilson, Anne, and John Graham. "Cell Culture." In Cell Biology Protocols, 51–85. Chichester, UK: John Wiley & Sons, Ltd, 2006. http://dx.doi.org/10.1002/0470033487.ch3.

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Gabrys, Beata, John L. Capinera, Jesusa C. Legaspi, Benjamin C. Legaspi, Lewis S. Long, John L. Capinera, Jamie Ellis, et al. "Cell Culture." In Encyclopedia of Entomology, 808. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6359-6_554.

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Ito, Masaki, and Kiyohiro Houkin. "Cell Culture." In Cell Therapy Against Cerebral Stroke, 49–72. Tokyo: Springer Japan, 2017. http://dx.doi.org/10.1007/978-4-431-56059-3_5.

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Messina, Antonietta, and Loredana De Bartolo. "Cell Culture." In Encyclopedia of Membranes, 1–3. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-642-40872-4_1199-1.

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Peters, J. H., E. Debus, H. Baumgarten, R. Würzner, M. Schulze, and Helga Gerlach. "Cell Culture." In Monoclonal Antibodies, 88–136. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-74532-4_5.

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Gooch, Jan W. "Cell Culture." In Encyclopedic Dictionary of Polymers, 880. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_13337.

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Yoon, Jeong-Yeol. "Cell Culture." In Tissue Engineering, 13–32. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-83696-2_2.

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Conference papers on the topic "Cell culture"

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Isu, Giuseppe, Diana Massai, Giulia Cerino, Diego Gallo, Cristina Bignardi, Alberto Audenino, and Umberto Morbiducci. "A Novel Perfusion Bioreactor for 3D Cell Culture in Microgravity Conditions." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14502.

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Cell suspension culture methods based on the generation of microgravity environment are widely used in regenerative medicine for (1) the production of native-like three-dimensional (3D) cell aggregates and engineered tissues [1,2,3], for (2) low cost scalable cell expansion and long-term cell viability maintenance [4,5], and for (3) guiding differentiation of stem cells (SCs) [6]. The generation of a microgravity environment for 3D cell cultures, mimicking the native environment, promotes spatial freedom, cell growth, cell-cell interaction and improves mass transfer and cell exposure to nutrients. Nowadays, microgravity cell cultures are obtained by using stirred or rotating bioreactors, but both devices suffer from limitations: stirring bioreactors generate non-physiological shear stresses, which could damage cultured cells, interfere with SC pluripotency, and limit reproducibility of the culture process; rotating bioreactors are expensive devices due to the complex technological solutions adopted for obtaining rotation [5].
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Berthier, R., A. Duperray, O. Valiron, M. Prenant, I. Newton, and A. Schweitzer. "MEGAKARYOCYTIC DEVELOPMENT IN LIQUID CULTURES OF CRYOPRESERVED LEUKOCYTE STEM CELL CONCENTRATES FROM CHRONIC MYELOGENOUS LEUKEMIA PATIENTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644622.

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The proliferation and differentiation of human megakaryocytes in liquid culture has been obtained using cryopreserved light density blood cell concentrates from chronic myelogenous leukemia (CML) patients. These cryopreserved leukocytes concentrates contain a large number of viable granulo-monocytic, erythroid and megakaryocytic committed stem cells. A high number of spontaneous megakaryocytic colonies was observed in semisolid cultures plated with the CML leukocytes concentrates. A liquid culture system using RPMI 1640 supplemented with 20% human plasma (HP) has been defined where maturing megakaryocytes make up 20 to 60% of the total cells after 14 days of incubation. The same cell suspension cultured in medium supplemented with 20% foetal calf serum (FCS) showed poor megakaryocytic cell development. The megakaryocytic nature of the cells produced in HP supplemented cultures was confirmed by cytological studies and indirect immunofluorescence labeling using monoclonal antibodies (MoAb) against membrane platelet GPIb and Ilbllla, and intracellular antigens like fibrinogen and von Willebrand factor.Ploidy of the cultured cells was studied after labeling with propidium iodide and the DNA fluorescence determined using the fluorescence activated cell sorter (FACSIV). Peaks of 8N, 16N and 32N cells were observed from HP supplemented cultures representing about 20% of the cells reacting with a GP11b111 a MoAb, while very few cells greater than 4N were observed in FCS supplemented cultures. The megakaryocytes produced in HP cultures could be further enriched by cell sorting on the FACSIV after labeling with an anti-IIbIIIa MoAb. Depending on the initial megakaryocytic concentration of the cells cultured, one to 2 é 106 megakaryocytes per hour could be harvested. Thus, cryopreserved CML blood stem cell concentrates seem to offer a reproducible source of human megakaryocytes which retain their capacity to proliferate and differentiate in liquid cultures supplemented with human plasma. These megakaryocytes can be used for the study of platelet glycoprotein biosynthesis as well as the regulation of megakaryocytopoiesis.
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Suganuma, Lisa, Hiromichi Fujie, Hiroki Sudama, Yoshihide Sato, Norimasa Nakamura, Kenji Suzuki, Yasuhiro Tanaka, and Nobuyuki Moronuki. "Nanostructure Processed on Culture Plate Improves Cell Adhesion." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53753.

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Ligaments and tendons have superior functions, but their healing capacities are limited. We have been developing a novel tissue-engineering technique for the repair of ligaments and tendons which involve stem cell-based self-assembled tissues (scSAT) derived from synovium[1]. For biological reconstruction of soft tissues, it is required for the scSAT to have high tensile strength. Our previous study indicted that, when the scSAT was cultured under high cell density condition, the tensile strength of the scSAT become higher than that cultured under low density condition[2]. However, the scSAT had a significant tendency to detach naturally from the culture dish with increasing cell density. Therefore, we expect that the mechanical property of the scSAT improves by enhancing the cell adhesion to culture plates. Previous studies suggested that nanostructure processed on culture dish affected cell adhesion [3, 4]. In the present study, nanostructure was processed on a silicon wafer using a nanoprocessing technology, and the structure was replicated to a polydimethylsiloxane (PDMS) plate. Human synovium-derived mesenchymal stem cells were cultured on the plate, and cell adhesion and morphological observation were performed.
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Hunter, N. R., I. R. MacGregor, J. Dawes, and D. S. Pepper. "MICROCARRIER CULTURE OF HUMAN ENDOTHELIAL CELL TYPES - A SOURCE OF METABOLITES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643348.

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The production of human endothelial cell secretory products in amounts sufficient for biochemical studies is largely restricted by the culture growth area. Conventional flat bed systems yield at best 20-30 x 106 cells per 180cm2 culture flask. To overcome this problem, cells may be grown on Cytodex 3 microcarriers allowing large numbers of cells to be grown and conditioned in small culture volumes. A typical microcarrier unit will contain 200-300 x 106 cells and may be expanded in excess of 1000 x 106 cells at confluence. High viability (95%) and recovery (70-80%) in sub-culturing of microcarrier to microcarrier culture can be achieved with careful management of culture conditions and brief exposure to enzymes.Human umbilical artery and vein, and saphenous vein endothelial cells were prepared and grogn on microcarrier cultures to cell populations of 200-450 x 106 cells and conditioned for 14 day periods in serum-free media.The production profiles of several endothelial cell proteins including thrombospondin (TSP), von Willebrand Factor (vWF) and issue plasminogen activator (t-PA) were measured by radioimmunoassay under these conditions, and demonstrate the use of microcarrier cultures in producing milligram quantities of engothelial cell protein. For example, a HUVEC culture of 200 x 106 cells conditioned with serum-free media for 14 days yielded a total of 6.9mg TSP, 0.7mg vWF and 48.9ug t-PA. In this laboratory one such application of the system was the purification of endothelial proteins in amounts sufficient for immunisation of mice prior to the production of monoclonal antibodies and for subsequent characterisation.
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Mignone, Lindsay F., Shirley Masand, Jeffrey D. Zahn, and David I. Shreiber. "A Simple, Cost-Effective Method to Improve Cell Viability in Microniche Culture Systems." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19189.

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Microfluidic networks are increasingly used to generate custom microenvironmental niches for cell culture and assays of cellular behavior. Perfusion systems are typically required to overcome diffusive limitations associated with culturing cells longer than a few hours when nutrient delivery, oxygen delivery and metabolic waste removal are required to maintain cell viability. In addition to the added complexity of experimental methods, perfusion systems can result in nonuniform nutrient delivery and subject cells to shear stresses, which may alter cell behavior and possibly cause cell death. In particular, when culturing cells within hydrogel scaffold-filled networks, as may be done in micro-tissue engineering, the need for perfusion culture also increases the likelihood of a destructive bubble entering the network. Moreover, analysis of micro-cultures frequently entails labelling with antibodies and/or fluorescent probes, which again requires controlled perfusion of the various reagents through the network. We have developed a simple technique to preserve cell viability and simplify labeling within microscale cultures without the need for perfusion. Instead of bonding a microfluidic network to glass, PDMS, or another impermeable substrate, the network is bonded to a semi-permeable microdialysis membrane, which allows free exchange of oxygen, proteins, nutrients, and waste between the microfluidic channels and culture media in static culture plates.
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Tsao, Yow-Min D., and Steve R. Gonda. "A New Technology for Three-Dimensional Cell Culture: The Hydrodynamic Focusing Bioreactor." In ASME 1999 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 1999. http://dx.doi.org/10.1115/imece1999-0578.

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Abstract The Hydrodynamic Focusing Bioreactor (HDFB) developed by NASA at the Johnson Space Center provides a unique hydrofocusing capability that simultaneously enables a low-shear culture environment and a unique hydrofocusing-based “herding” of suspended cells, cell aggregates, and air bubbles. The HDFB is a rotating dome-shaped cell culture vessel with a centrally located sampling port and an internal rotating viscous spinner attached to a rotating base. The vessel and viscous spinner can rotate at different speeds and in either the same or different directions. Adjusting the differential rotation rate between the vessel and spinner results in a controllable hydrodynamic focusing force. The resultant hydrodynamic force suspends the cells in a low-shear fluid environment that supports the formation of delicate three-dimensional tissue assemblies. Both suspension and anchorage-dependent cells have been successfully cultured.
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Shimomura, Yuya, Shoichiro Kanno, Kenta Shimba, Yoshitaka Miyamoto, and Tohru Yagi. "Position-controllable cell culture substrate for adherent cells." In 2023 15th Biomedical Engineering International Conference (BMEiCON). IEEE, 2023. http://dx.doi.org/10.1109/bmeicon60347.2023.10321824.

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Suzuki, Kei, Toshihiko Shiraishi, Shin Morishita, and Hiroshi Kanno. "Effects of Mechanical Vibration on Proliferation and Differentiation of Neural Stem Cells." In ASME 2008 International Mechanical Engineering Congress and Exposition. ASMEDC, 2008. http://dx.doi.org/10.1115/imece2008-66831.

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Neural stem cells have been studied to promote neurogenesis in regenerative therapy. The control of differentiation of neural stem cells to nerve cells and the increase of the number of nerve cells are needed. For the purpose of them, it is important to investigate not only chemical factors but also mechanical factors such as hydrostatic pressure in brain and mechanical vibration in walking. In this study, sinusoidal inertia force was applied to cultured neural stem cells and the effects of mechanical vibration on the cells were investigated. After the cells were cultured in culture plates for one day and adhered on the cultured plane, vibrating group of the culture plates was set on an aluminum plate attached to an exciter and cultured under sinusoidal excitation for 24 hours a day during 26 days. The amplitude of the acceleration on the culture plate was set to 0.25 G and the frequency was set to 25 Hz. The time evolution of cell density was obtained by counting the number of cells at every 3 or 4 days. The expression of Akt, phosphorylated Akt (p-Akt), MAPK, and phosphorylated MAPK (p-MAPK) was detected by western blotting analysis at 7 days of culture to understand the mechanism of cell proliferation. Akt and MAPK are part of signaling pathways in relation to cell proliferation. The phosphorylation of Akt suppresses apoptosis and the phosphorylation of MAPK activates cell division. The gene expression of MAP-2, NFH, GFAP, and nestin was detected by real-time RT-PCR analysis at 7 days of culture to obtain a ratio of differentiation of neural stem cells to nerve or glia cells. MAP-2 and NFH are nerve cell markers, GFAP is a glia cell marker, and nestin is a stem cell marker. The results obtained are as follows. The cell density of the vibrating group was three times higher than that of the non-vibrating group at 26 days of culture. p-Akt was enhanced by the mechanical vibration while p-MAPK was not. There is no significant difference of the gene expression level of MAP-2, NFH, GFAP, and nestin between the vibrating and non-vibrating groups. These results suggest that the mechanical vibration promotes the proliferation of neural stem cells and its cause is likely the suppression of apoptosis but not the activation of cell division, and that the mechanical vibration at the experimental condition does not affect the differentiation of neural stem cells to nerve or glia cells.
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Ma, Liang, Lei Gao, Yichen Luo, Huayong Yang, Bin Zhang, Changchun Zhou, JinGyu Ock, and Wei Li. "Flow Analysis of a Porous Polymer-Based Three-Dimensional Cell Culture Device for Drug Screening." In ASME 2018 13th International Manufacturing Science and Engineering Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/msec2018-6313.

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A porous polymer-based three-dimensional (3D) cell culture device has been developed as an in vitro tissue model system for the cytotoxicity of anticancer drug test. The device had two chambers connected in tandem, each loaded with a 3D scaffold made of highly biocompatible poly (lactic acid) (PLA). Hepatoma cells (HepG2) and glioblastoma multiforme (GBM) cancer cells were cultured in the two separate porous scaffolds. A peristaltic pump was adopted to realize a perfusion cell culture. In this study, we focus on cell viability inside the 3D porous scaffolds under flow-induced shear stress effects. A flow simulation was conducted to predict the shear stress based on a realistic representation of the porous structure. The simulation results were correlated to the cell variability measurements at different flow rates. It is shown that the modeling approach presented in this paper can be useful for shear stress predication inside porous scaffolds and the computational fluid dynamics model can be an effective way to optimize the operation parameters of perfused 3D cell culture devices.
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Lai, Yi-Han, and Shih-Kang Fan. "Electromolding for 3D cell culture." In 2015 IEEE 10th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS). IEEE, 2015. http://dx.doi.org/10.1109/nems.2015.7147424.

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Reports on the topic "Cell culture"

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Oldberg, Nick. A Calculator for Cell Culture Plating. ResearchHub Technologies, Inc., April 2024. http://dx.doi.org/10.55277/researchhub.mg3bsfrd.

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Leung, Diana. A Call for Context (in Cell Culture). New Science, September 2022. http://dx.doi.org/10.56416/021uwn.

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Brudos, Emma, Miranda Nelson, and David Estrada. Optimizing Three-Dimensional Bioprinting for Cell Culture Scaffolds. Peeref, July 2022. http://dx.doi.org/10.54985/peeref.2207p5266153.

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Malik, Abir, D. Lam, H. A. Enright, S. K. G. Peters, B. Petkus, and N. O. Fischer. Characterizing the Phenotypes of Brain Cells in a 3D Hydrogel Cell Culture Model. Office of Scientific and Technical Information (OSTI), August 2018. http://dx.doi.org/10.2172/1466140.

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Scott, C. D., and D. K. Dougall. Plant cell tissue culture: A potential source of chemicals. Office of Scientific and Technical Information (OSTI), August 1987. http://dx.doi.org/10.2172/5938126.

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Ostry, M. E., and K. T. Ward. Bibliography of Populus cell and tissue culture. St. Paul, MN: U.S. Department of Agriculture, Forest Service, North Central Forest Experiment Station, 1991. http://dx.doi.org/10.2737/nc-gtr-146.

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Novaro, Virginia, and Mina BIssell. A Cell Culture Model for Understanding Estrogen Receptor Regulation in Normal and Malignant Cells. Fort Belvoir, VA: Defense Technical Information Center, October 1998. http://dx.doi.org/10.21236/ada373393.

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Mort, A. (The structure of pectins from cotton suspension culture cell walls). Office of Scientific and Technical Information (OSTI), January 1990. http://dx.doi.org/10.2172/7003410.

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Evangelatov, Alexander, Diana Naidenova, George Georgiev, Albena Momchilova, and Roumen Pankov. Effects of Hyperglycemia on Wound Healing in Threedimensional Cell Culture. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, June 2021. http://dx.doi.org/10.7546/crabs.2021.06.08.

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Zhou, Hong, Aaron Mack, Gitanjali Talreja, Josia Assmies, Stefan Minning, Andreas Unsoeld, John Bobiak, et al. BEST PRACTICE GUIDE FOR PREPARATION OF CELL CULTURE MEDIA SOLUTION. BioPhorum, April 2021. http://dx.doi.org/10.46220/2021ds001.

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