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Journal articles on the topic 'CDNA library construction'

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Published: 4 June 2021

Last updated: 19 February 2022

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1

Fedchenko, V. I., A. A. Kaloshin, and A. E. Medvedev. "A novel vector for construction of cDNA library." Biomeditsinskaya Khimiya 56, no. 3 (2010): 329–41. http://dx.doi.org/10.18097/pbmc20105603329.

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A new original vector pEM-(dT)40(f+) has been prepared. It can be used for cDNA library construction from polyadenylated mRNA, isolated from various sources. The pGEM-(dT)40f(+) is initially transformed into single stranded and then into a linear form and its (dT)40 tail at 3'-end is used as the vector-primer for synthesis of the first strand cDNA. The use of a synthetic oligonucleotide complementary to the vector and recombinant DNA results in vector cyclization and synthesis of the second strand cDNA. This approach significantly simplifies cDNA library construction, it does not require PCR reaction (which can induce artifact mutations in cDNA sequences) and restrictase treatment.

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2

Nomura, Takashi, Tokuya Takahashi, Kohji Hara, and Hiroyoshi Nohara. "Construction of a bovine gingival cDNA library." Japanese Journal of Oral Biology 33, no. 6 (1991): 560–66. http://dx.doi.org/10.2330/joralbiosci1965.33.560.

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3

Oh, Gyu-Dong, Eun-Jo Shim, Sang-Jin Jun, and Young-Doo Park. "Construction of cDNA Library and EST Analysis Related to Seed-hair Characteristics in Carrot." Korean Journal of Horticultural Science and Technology 31, no. 6 (December 31, 2013): 782–89. http://dx.doi.org/10.7235/hort.2013.13108.

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4

Wu, Wei, Yongfeng Sun, Tongao Yang, Jingtao Hu, Zhe Hao, Yujian Sui, Yingying Fu, Lu Chen, and Haiyang Zhu. "Construction of Full-Length Goose Muscle cDNA Library." Journal of Animal and Veterinary Advances 11, no. 12 (December 1, 2012): 2106–9. http://dx.doi.org/10.3923/javaa.2012.2106.2109.

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5

Huang, Yao, Chuanling Qiao, Jing Wang, and Naihu Wu. "CONSTRUCTION OF cDNA LIBRARY FROM INSECTICIDE-RESISTANT HOUSEFLIES." Insect Science 1, no. 2 (June 1994): 191–93. http://dx.doi.org/10.1111/j.1744-7917.1994.tb00211.x.

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6

Roeder, T. "Solid-phase cDNA library construction, a versatile approach." Nucleic Acids Research 26, no. 14 (July 1, 1998): 3451–52. http://dx.doi.org/10.1093/nar/26.14.3451.

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7

Soares, M. B., M. F. Bonaldo, P. Jelene, L. Su, L. Lawton, and A. Efstratiadis. "Construction and characterization of a normalized cDNA library." Proceedings of the National Academy of Sciences 91, no. 20 (September 27, 1994): 9228–32. http://dx.doi.org/10.1073/pnas.91.20.9228.

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8

Patanjali, S. R., S. Parimoo, and S. M. Weissman. "Construction of a uniform-abundance (normalized) cDNA library." Proceedings of the National Academy of Sciences 88, no. 5 (March 1, 1991): 1943–47. http://dx.doi.org/10.1073/pnas.88.5.1943.

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9

Sui, Zhenghong, and Klaus V. Kowallik. "Construction of cDNA library of Pyrocystis lunula (Pyrophyta)." Journal of Ocean University of China 3, no. 2 (October 2004): 141–44. http://dx.doi.org/10.1007/s11802-004-0024-9.

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10

Bellemare, Guy, Claude Potvin, and Diane Bergeron. "High-yield method for directional cDNA library construction." Gene 98, no. 2 (February 1991): 231–35. http://dx.doi.org/10.1016/0378-1119(91)90178-e.

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11

Kansal, Rekha, T. P. Ramkumar, and K. R. Koundal. "Construction and Screening of Chickpea cDNA Library with Heterologous Legumin cDNA Probe." Journal of Plant Biochemistry and Biotechnology 1, no. 1 (January 1992): 69–71. http://dx.doi.org/10.1007/bf03262899.

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12

Fang, Shang Ling, Jin Yan, Mao Bin Chen, Dong Sheng Xue, and Jing Hua Cao. "Construction of cDNA Library of Hansenula anomala." Applied Mechanics and Materials 665 (October 2014): 363–66. http://dx.doi.org/10.4028/www.scientific.net/amm.665.363.

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To investigate the molecular mechanism of ester-producing of acetate yeast, a cDNA library was constructed based on Switching Mechanism At 5’ end of the RNA Transcript (SMART) technique using Construction Kit. The result of the test showed that the primary constructed cDNA library had a cloning capacity of 3×106and the length of the inserted fragment was between 1-3kb,the coverage of cDNA library was 74.07%. The quality of the library could meet the requirements.

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13

Welsh, John, Jeh-Ping Liu, and Argiris Efstratiadis. "Cloning of PCR-amplified total cDNA: Construction of a mouse oocyte cDNA library." Gene Analysis Techniques 7, no. 1 (February 1990): 5–17. http://dx.doi.org/10.1016/0735-0651(90)90038-h.

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14

Kohchi, Takayuki, Kotomi Fujishige, and Kanji Ohyama. "Construction of an equalized cDNA library from Arabidopsis thaliana." Plant Journal 8, no. 5 (November 1995): 771–76. http://dx.doi.org/10.1046/j.1365-313x.1995.08050771.x.

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15

Inagaki, Atsuko, Yoshitaka Takano, Yasuyuki Kubo, Kazuyuki Mise, and Iwao Furusawa. "Construction of an equalized cDNA library fromColletotrichum lagenariumand its application to the isolation of differentially expressed genes." Canadian Journal of Microbiology 46, no. 2 (February 1, 2000): 150–58. http://dx.doi.org/10.1139/w99-119.

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To establish an efficient screening system for differentially expressed genes of a phytopathogenic fungus Colletotrichum lagenarium, we constructed an equalized (normalized) cDNA library from C. lagenarium and used this library for differential screening. For the isolation of genes involved in infection-related developments of conidia, conidia undergoing appressorium differentiation were selected as the source of materials for construction of the cDNA library. The equalization of cDNA was performed twice using a kinetic method, and the products were cloned into a plasmid vector. Colony hybridization with nine probes of different abundance showed a reduction in abundance variation from at least 276-fold in the original library to 10-fold in the equalized cDNA library, which demonstrated that the cDNA was successfully equalized. By differential hybridization of 1900 cDNA clones in the equalized cDNA library and RNA blot analysis of candidate clones, we identified 11 independent cDNA clones, designated CAD1 through CAD11, that were expressed in appressorium-differentiating conidia, but not in vegetative mycelia. The transcripts of CAD1 and CAD2 hardly accumulated in preincubated conidia, whereas those of CAD3 and CAD4 accumulated highly and slightly, respectively. The amount of the four CAD transcripts increased at the early stage of the appressorium formation process. Sequence analysis of CAD1 revealed that CAD1 would encode for 101 amino acid polypeptides, which showed homology to metallothioneins. Deduced amino acid sequence of CAD2 would encode 278 amino acid polypeptides, and showed high homology to genes in aflatoxin, and sterigmatocystin gene clusters of Aspergillus parasiticus and A. nidulans, respectively. Key words: equalized cDNA library, differential screening, Colletotrichum lagenarium, appressorium formation, CAD genes.

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16

Hillgenberg, Moritz, Christian Hofmann, Herbert Stadler, and Peter Löser. "High-Efficiency System for the Construction of Adenovirus Vectors and Its Application to the Generation of Representative Adenovirus-Based cDNA Expression Libraries." Journal of Virology 80, no. 11 (June 1, 2006): 5435–50. http://dx.doi.org/10.1128/jvi.00218-06.

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ABSTRACT We here describe a convenient system for the production of recombinant adenovirus vectors and its use for the construction of a representative adenovirus-based cDNA expression library. The system is based on direct site-specific insertion of transgene cassettes into a replicating donor virus. The transgene is inserted into a donor plasmid containing the viral 5′ inverted terminal repeat, the complete viral packaging signal, and a single loxP site. The plasmid is then transfected into a Cre recombinase-expressing packaging cell line that has been infected with a donor virus containing a partially deleted packaging signal flanked by loxP sites. Cre recombinase, by two steps of action, sequentially catalyzes the generation of a nonpackageable donor virus acceptor substrate and the generation of the desired recombinant adenovirus vector. Due to its growth impairment, residual donor virus can efficiently be counterselected during amplification of the recombinant adenovirus vector. By using this adenovirus construction system, a plasmid-based human liver cDNA library was converted by a single step into an adenovirus-based cDNA expression library with about 106 independent adenovirus clones. The high-titer purified library was shown to contain about 44% of full-length cDNAs with an average insert size of 1.3 kb. cDNAs of a gene expressed at a high level (human α1-antitrypsin) and a gene expressed at a relatively low level (human coagulation factor IX) in human liver were isolated from the adenovirus-based library using an enzyme-linked immunosorbent assay-based screening procedure.

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17

Takahashi, Naomi, and Minoru S. H. Ko. "Toward a Whole cDNA Catalog: Construction of an Equalized cDNA Library from Mouse Embryos." Genomics 23, no. 1 (September 1994): 202–10. http://dx.doi.org/10.1006/geno.1994.1478.

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18

Shimokawa, H., Y. Ogata, S. Sasaki, M. E. Sobel, C. I. Mcquillan, J. D. Termine, and M. F. Young. "Molecular Cloning of Bovine Amelogenin cDna." Advances in Dental Research 1, no. 2 (December 1987): 293–97. http://dx.doi.org/10.1177/08959374870010022001.

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Molecular cloning of a bovine amelogenin cDNA was accomplished by construction of a cDNA expression library (λgt11 cDNA library) from the bovine ameloblast mRNA and then screening of the library with antibodies to bovine amelogenins. The complete primary structure of an amelogenin was deduced from cloned cDNA. One of the cDNA clones isolated from a bovine ameloblast phage λgt11 library had an 864-base-pair-long insert that encoded a protein with 216 amino acid residues. This cDNA clone appears to represent the complete coding region of amelogenin mRNA, including a putative AUG initiation codon and a signal peptide sequence. The predicted bovine amelogenin sequence has 87% amino acid homology with murine amelogenin.

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19

Enroth, Christel Hougård, Annaleigh Ohrt Fehler, Line Dahl Poulsen, and Jeppe Vinther. "Excess primer degradation by Exo I improves the preparation of 3′ cDNA ligation-based sequencing libraries." BioTechniques 67, no. 3 (September 2019): 110–16. http://dx.doi.org/10.2144/btn-2018-0178.

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RNA sequencing library construction using single-stranded ligation of a DNA adapter to 3′ ends of cDNAs often produces primer–adapter byproducts, which compete with cDNA–adapter ligation products during library amplification and, therefore, reduces the number of informative sequencing reads. We find that Escherichia coli Exo I digestion efficiently and selectively removes surplus reverse transcription primer and thereby reduces the primer–adapter product contamination in 3′ cDNA ligation-based sequencing libraries, including small RNA libraries, which are typically similar in size to the primer–adapter products. We further demonstrate that Exo I treatment does not lead to trimming of the cDNA 3′ end when duplexed with the RNA template. Exo I digestion is easy to perform and implement in other protocols and could facilitate a more widespread use of 3′ cDNA ligation for sequencing-based applications.

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20

Chen, Lei, Lixue Cao, Longhai Zhou, Yudong Jing, Zuozhou Chen, Cheng Deng, Yu Shen, and Liangbiao Chen. "Trehalose as a good candidate for enriching full-length cDNAs in cDNA library construction." Journal of Biotechnology 127, no. 3 (January 2007): 402–7. http://dx.doi.org/10.1016/j.jbiotec.2006.07.033.

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21

Hu, Li, Ya-e. Zhao, Dong-ling Niu, Rui Yang, and Ji-hui Zeng. "The Construction of Full-Length cDNA Library for Otodectes cynotis." Acta Parasitologica 64, no. 2 (March 12, 2019): 251–56. http://dx.doi.org/10.2478/s11686-019-00034-y.

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22

Radchuk, V., Ya V. Pirko, S. V. Isayenkov, A. I. Yemets, and Ya B. Blume. "cDNA library construction from meristematic tissue of finger millet panicle." Cytology and Genetics 48, no. 5 (September 2014): 273–78. http://dx.doi.org/10.3103/s0095452714050089.

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23

Isayenkov, S., and F. J. M. Maathuis. "Construction and applications of a mycorrhizal arbuscular specific cDNA library." Cytology and Genetics 50, no. 2 (March 2016): 79–88. http://dx.doi.org/10.3103/s0095452716020043.

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24

Lonneborg, A., P. Sharma, and P. Stougaard. "Construction of subtractive cDNA library using magnetic beads and PCR." Genome Research 4, no. 4 (February 1, 1995): S168—S176. http://dx.doi.org/10.1101/gr.4.4.s168.

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25

Houge, G. "Simplified construction of a subtracted cDNA library using asymmetric PCR." Genome Research 2, no. 3 (February 1, 1993): 204–9. http://dx.doi.org/10.1101/gr.2.3.204.

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26

Han, Xue Feng, Jun Luo, Ning Wu, Kanyand Matand, Bao Jin Yang, Hui Juan Wu, Li Juan Zhang, and Hai Bin Wang. "Construction and Characterization of a Goat Mammary Gland cDNA Library." Molecular Biotechnology 38, no. 3 (November 30, 2007): 187–93. http://dx.doi.org/10.1007/s12033-007-9020-9.

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27

EL-MATBOULI, M., and H. SOLIMAN. "Construction and screening of a cDNA library from the triactinomyxon spores ofMyxobolus cerebralis, the causative agent of salmonid Whirling Diseases." Parasitology 132, no. 4 (January 3, 2006): 467–77. http://dx.doi.org/10.1017/s0031182005009522.

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The ZAP Express cDNA library was constructed using mRNA extracted from the triactinomyxon spores ofMyxobolus cerebralis. First-strand cDNA was synthesized using Moloney Murine leukaemia virus reverse transcriptase. Following second-strand cDNA synthesis, the double-stranded cDNA was digested withXhoI restriction enzyme, cDNA fragments less than 400 bp were removed and the remaining cDNA was ligated with the lambda ZAP Express vector. The recombinants were packagedin vitrousing Gigapack III gold packaging extract. The primary cDNA library titre contained 0·5×106clones, with 97% recombinant and only 3% non-recombinant clones. The cDNA library was then screened using the anti-triactinomyxon antibodies. Positive clones were selected and re-screened twice more to give a final selection of 526 clones. One clone (46-5) was selected randomly and subjected toin vivo excision of the pBK-CMV phagemid from the ZAP express vector. The sequence of the entire clone was obtained using rapid amplification of the cDNA ends. A search of the clone sequence against GenBank revealed that it related to ribosomal protein L23 and it had a high percentage similarity to this protein from different species. A conserved domain for ribosomal protein L23 was also identified in the clone sequence.

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28

Belyavsky, A., T. Vinogradova, and K. Rajewsky. "PCR-based cDNA library construction: general cDNA libraries at the level of a few cells." Nucleic Acids Research 17, no. 14 (1989): 5883. http://dx.doi.org/10.1093/nar/17.14.5883.

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29

Belyavsky, A., T. Vinogradova, and K. Rajewsky. "PCR-based cDNA library construction: general cDNA libraries at the level of a few cells." Nucleic Acids Research 17, no. 8 (1989): 2919–32. http://dx.doi.org/10.1093/nar/17.8.2919.

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30

Xie, Yong-Fang, Bo-Chu Wang, Biao Li, Ying-Fan Cai, Lei Xie, Yu-Xian Xia, Ping-An Chang, and Huai-Zhong Jiang. "Construction of cDNA library of cotton mutant (Xiangmian-18) library during gland forming stage." Colloids and Surfaces B: Biointerfaces 60, no. 2 (November 2007): 258–63. http://dx.doi.org/10.1016/j.colsurfb.2007.06.020.

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31

Zhao, Gui hua, Ye Liu, Yun tang Cheng, Qing song Zhao, Xiao Qiu, Chao Xu, Ting Xiao, Song Zhu, Gong zhen Liu, and Kun Yin. "Primary culture of cat intestinal epithelial cells in vitro and the cDNA library construction." Acta Parasitologica 63, no. 2 (June 26, 2018): 360–67. http://dx.doi.org/10.1515/ap-2018-0041.

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AbstractFelids are the only definitive hosts ofToxoplasma gondii. To lay a foundation for screening theT.gondii-felids interaction factors, we have developed a reproducible primary culture method for cat intestinal epithelial cells (IECs). The primary IECs were isolated from a new born cat’s small intestine jejunum region without food ingress, and respectivelyin vitrocultured by tissue cultivation and combined digestion method with collagenase XI and dispase I, then purified by trypsinization. After identification, the ds cDNA of cat IECs was synthesized for constructing pGADT7 homogenization three-frame plasmid, and transformed into the yeast Y187 for generating the cDNA library. Our results indicated that cultivation of primary cat IECs relays on combined digestion to form polarized and confluent monolayers within 3 days with typical features of normal epithelial cells. The purified cells cultured by digestion method were identified to be nature intestinal epithelial cells using immunohistochemical analysis and were able to maintain viability for at least 15 passages. The homogenizable ds cDNA, which is synthesized from the total RNA extracted from our cultured IECs, distributed among 0.5–2.0 kb, and generated satisfying three-frame cDNA library with the capacity of 1.2 × 106and the titer of 5.2 × 107pfu/mL. Our results established an optimal method for the culturing and passage of cat IECs modelin vitro, and laid a cDNA library foundation for the subsequent interaction factors screening by yeast two-hybrid.

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32

Yin, Kun, Chao Xu, Gui-Hua Zhao, Ye Liu, Ting Xiao, Song Zhu, and Ge Yan. "Construction of C35 gene bait recombinants and T47D cell cDNA library." BioScience Trends 11, no. 5 (2017): 550–56. http://dx.doi.org/10.5582/bst.2017.01161.

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33

Hosoya, Shiki, Kazutoshi Tamura, Kazushi Nomura, and Yoshimitsu Abiko. "CONSTRUCTION OF SUBTRACTED OSTEOBLAST cDNA LIBRARY WITH LASER-IRRADIATION-ENHANCED TRANSCRIPTION." LASER THERAPY 9, no. 3 (1997): 107–13. http://dx.doi.org/10.5978/islsm.9.107.

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34

Yamagishi, Junya, Hiroyuki Wakaguri, Sumio Sugano, Suguru Kawano, Kozo Fujisaki, Chihiro Sugimoto, Junichi Watanabe, Yutaka Suzuki, Isao Kimata, and Xuenan Xuan. "Construction and analysis of full-length cDNA library of Cryptosporidium parvum." Parasitology International 60, no. 2 (June 2011): 199–202. http://dx.doi.org/10.1016/j.parint.2011.03.001.

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35

Song, Tieli, Tingting Wu, Fulan Wei, Ang Li, Fu Wang, Yilin Xie, Dayong Liu, et al. "Construction of a cDNA library for miniature pig mandibular deciduous molars." BMC Developmental Biology 14, no. 1 (2014): 16. http://dx.doi.org/10.1186/1471-213x-14-16.

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36

Ohara, O. "Directional cDNA library construction assisted by the in vitro recombination reaction." Nucleic Acids Research 29, no. 4 (February 15, 2001): 22e—22. http://dx.doi.org/10.1093/nar/29.4.e22.

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37

Xu, Lei, Jingqi Li, Li Liu, Lixia Lu, Jingxia Gao, and Xueli Li. "Construction of equalized short hairpin RNA library from human brain cDNA." Journal of Biotechnology 128, no. 3 (February 20, 2007): 477–85. http://dx.doi.org/10.1016/j.jbiotec.2006.11.013.

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38

Wilcox, Edward R., and Jörgen Fex. "Construction of a cDNA library from microdissected guinea pig crista ampullaris." Hearing Research 73, no. 1 (February 1994): 65–66. http://dx.doi.org/10.1016/0378-5955(94)90283-6.

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39

Schraml, Peter, Robert Shipman, Peter Stulz, and Christian U. Ludwig. "cDNa subtraction library construction using a magnet-assisted subtraction technique (MAST)." Trends in Genetics 9, no. 3 (March 1993): 70–71. http://dx.doi.org/10.1016/0168-9525(93)90216-5.

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40

Sasaki, Yasnory F., Dai Ayusawa, and Michio Oishi. "Construction of a normalized cDNA library by introduction of a semi-solid mRNA-cDNA hybridization system." Nucleic Acids Research 22, no. 6 (1994): 987–92. http://dx.doi.org/10.1093/nar/22.6.987.

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41

D'Souza, Cynthia R., Ken V. Deugau, and John H. Spencer. "A simplified procedure for cDNA and genomic library construction using nonpalindromic oligonucleotide adaptors." Biochemistry and Cell Biology 67, no. 4-5 (April 1, 1989): 205–9. http://dx.doi.org/10.1139/o89-031.

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The properties and characteristics of oligonucleotide adaptors for use in a simplified procedure for the construction of cDNA and genomic DNA libraries are described. The adaptors are suitable for joining to blunt ended cDNA or sheared genomic DNA, and then to the cohesive ends of restriction sites in vectors. Each adaptor consists of two oligonucleotides with complementary but nonpalindromic sequences that include an internal restriction site, a 5′ phosphorylated blunt end, and an overlapping or staggered 5′ hydroxylated end corresponding to a restriction endonuclease site in a vector of choice. Ligation of the blunt end to high molecular weight target DNA proceeds efficiently and there is no tandem concatenation of the adaptor. Insertion into the appropriate vector only requires ligation of the cohesive ends. There is no requirement for methylation, restriction enzyme cleavage, G-C tailing, or denaturation after ligation of the adaptor to the target DNA, all characteristics of other procedures.Key words: library, genomic, cDNA, oligonucleotides, adaptors.

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42

薛, 雯. "Construction of a T7 Phage Display cDNA Library from Lung Cancer of Smokers." Hans Journal of Biomedicine 02, no. 02 (2012): 15–18. http://dx.doi.org/10.12677/hjbm.2012.22004.

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43

Kobayashi, H., H. Takahashi, K. Oyanagi, F. Ikuta, T. Miyatake, and S. Tsuji. "Construction of spinal cord cDNA library and application for subtractive cloning of spinal cord-specific cDNAs." Journal of Molecular Neuroscience 3, no. 2 (June 1991): 59–64. http://dx.doi.org/10.1007/bf02885526.

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44

Xu, Yan, and Yuejin Wang. "Construction of a cDNA Library of Vitis pseudoreticulata Native to China Inoculated with Uncinula necator and the Analysis of Potential Defense-related Expressed Sequence Tags." HortScience 41, no. 4 (July 2006): 993C—993. http://dx.doi.org/10.21273/hortsci.41.4.993c.

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Expressed sequence tags (ESTs) constitute a rapid and informative strategy for studying gene-expression profiles of specific stages of annual and perennial plant species. Compared with annual plants, the NCBI database has very little sequence information from perennial plant species. To date, only ∼145 ESTs of Vitis pseudoreticulata W.T. Wang have been deposited in databases. This is insufficient to understand the biology and development of this species. In this report, a cDNA library constructed from young leaf inoculated with powdery mildew pathogen [Uncinula necator (Schw.) Burr.] of Chinese wild Vitis pseudoreticulata. Leaf was harvested at various times after inoculation for total RNA extraction, which was used to generate ESTs. In our study, 107 cDNA clones were sequenced either from 5' or 3' end of the cDNAs. Among them, 60 unigenes (56%) were functionally characterized by the BLASTX matches to known function proteins, and 20 unigenes (18.6 %) matched significantly with those having unknown function in the public databases. The remaining 27 unigenes (25.2%) failed to show significant homology to any proteins in the public databases, suggesting that they represent novel sequences. Some functional genes identified from the cDNA library to be potentially associated with plant defence-related responses are discussed.

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45

DING, Jun, Wei SUN, and Yaqing CHANG. "Construction of cDNA library and partial ESTs analysis of Strongylo-centrotus intermedius." Journal of Fishery Sciences of China 18, no. 1 (August 7, 2013): 222–29. http://dx.doi.org/10.3724/sp.j.1118.2011.00222.

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46

Zhang, Ling, Fu-Guang Li, Chuan-Liang Liu, Chao-Jun Zhang, and Xue-Yan Zhang. "Construction and analysis of cotton (Gossypium arboreum L.) drought-related cDNA library." BMC Research Notes 2, no. 1 (2009): 120. http://dx.doi.org/10.1186/1756-0500-2-120.

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47

Qi-kang, GAO, and HU Cui. "CONSTRUCTION OF A cDNA LIBRARY OF HOST RECOGNITION KAIROMONE FOR TELENOMUS THEOPHILAE." Insect Science 9, no. 1 (March 2002): 35–39. http://dx.doi.org/10.1111/j.1744-7917.2002.tb00140.x.

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48

ÜRMÉNYI, TURÁN P., MARIA F. BONALDO, MARCELO B. SOARES, and EDSON RONDINELLI. "Construction of a Normalized cDNA Library for the Trypanosoma cruzi Genome Project." Journal of Eukaryotic Microbiology 46, no. 5 (September 1999): 542–44. http://dx.doi.org/10.1111/j.1550-7408.1999.tb06072.x.

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49

Yang, Fan, Haidong Tan, Yongjin Zhou, Xinping Lin, and Sufang Zhang. "High-Quality RNA Preparation from Rhodosporidium toruloides and cDNA Library Construction Therewith." Molecular Biotechnology 47, no. 2 (August 20, 2010): 144–51. http://dx.doi.org/10.1007/s12033-010-9322-1.

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50

Gang, Chen, Zhang Wanggang, Fu Jie, Cao Xingmei, Zhao Wanhong, Han Yueheng, Zhao Aizhi, LI Fuyang, Liu Xinping, and Yao Libo. "Construction and significance of directional expression cDNA library from human NB4 cells." Journal of Huazhong University of Science and Technology [Medical Sciences] 24, no. 1 (February 2004): 52–54. http://dx.doi.org/10.1007/bf02830705.

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