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Academic literature on the topic 'CDNA library construction'

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Published: 4 June 2021
Last updated: 19 February 2022

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Journal articles on the topic "CDNA library construction"

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Fedchenko, V. I., A. A. Kaloshin, and A. E. Medvedev. "A novel vector for construction of cDNA library." Biomeditsinskaya Khimiya 56, no. 3 (2010): 329–41. http://dx.doi.org/10.18097/pbmc20105603329.

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A new original vector pEM-(dT)40(f+) has been prepared. It can be used for cDNA library construction from polyadenylated mRNA, isolated from various sources. The pGEM-(dT)40f(+) is initially transformed into single stranded and then into a linear form and its (dT)40 tail at 3'-end is used as the vector-primer for synthesis of the first strand cDNA. The use of a synthetic oligonucleotide complementary to the vector and recombinant DNA results in vector cyclization and synthesis of the second strand cDNA. This approach significantly simplifies cDNA library construction, it does not require PCR reaction (which can induce artifact mutations in cDNA sequences) and restrictase treatment.
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Nomura, Takashi, Tokuya Takahashi, Kohji Hara, and Hiroyoshi Nohara. "Construction of a bovine gingival cDNA library." Japanese Journal of Oral Biology 33, no. 6 (1991): 560–66. http://dx.doi.org/10.2330/joralbiosci1965.33.560.

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Oh, Gyu-Dong, Eun-Jo Shim, Sang-Jin Jun, and Young-Doo Park. "Construction of cDNA Library and EST Analysis Related to Seed-hair Characteristics in Carrot." Korean Journal of Horticultural Science and Technology 31, no. 6 (December 31, 2013): 782–89. http://dx.doi.org/10.7235/hort.2013.13108.

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Wu, Wei, Yongfeng Sun, Tongao Yang, Jingtao Hu, Zhe Hao, Yujian Sui, Yingying Fu, Lu Chen, and Haiyang Zhu. "Construction of Full-Length Goose Muscle cDNA Library." Journal of Animal and Veterinary Advances 11, no. 12 (December 1, 2012): 2106–9. http://dx.doi.org/10.3923/javaa.2012.2106.2109.

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Huang, Yao, Chuanling Qiao, Jing Wang, and Naihu Wu. "CONSTRUCTION OF cDNA LIBRARY FROM INSECTICIDE-RESISTANT HOUSEFLIES." Insect Science 1, no. 2 (June 1994): 191–93. http://dx.doi.org/10.1111/j.1744-7917.1994.tb00211.x.

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Roeder, T. "Solid-phase cDNA library construction, a versatile approach." Nucleic Acids Research 26, no. 14 (July 1, 1998): 3451–52. http://dx.doi.org/10.1093/nar/26.14.3451.

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Soares, M. B., M. F. Bonaldo, P. Jelene, L. Su, L. Lawton, and A. Efstratiadis. "Construction and characterization of a normalized cDNA library." Proceedings of the National Academy of Sciences 91, no. 20 (September 27, 1994): 9228–32. http://dx.doi.org/10.1073/pnas.91.20.9228.

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Patanjali, S. R., S. Parimoo, and S. M. Weissman. "Construction of a uniform-abundance (normalized) cDNA library." Proceedings of the National Academy of Sciences 88, no. 5 (March 1, 1991): 1943–47. http://dx.doi.org/10.1073/pnas.88.5.1943.

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Sui, Zhenghong, and Klaus V. Kowallik. "Construction of cDNA library of Pyrocystis lunula (Pyrophyta)." Journal of Ocean University of China 3, no. 2 (October 2004): 141–44. http://dx.doi.org/10.1007/s11802-004-0024-9.

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Bellemare, Guy, Claude Potvin, and Diane Bergeron. "High-yield method for directional cDNA library construction." Gene 98, no. 2 (February 1991): 231–35. http://dx.doi.org/10.1016/0378-1119(91)90178-e.

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Dissertations / Theses on the topic "CDNA library construction"

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Holm, Kora. "Construction of a cDNA library for the vine mealybug, Planococcus ficus (Signoret)." Thesis, Stellenbosch : Stellenbosch University, 2008. http://hdl.handle.net/10019.1/4083.

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Thesis (MSc (Genetics))--Stellenbosch University, 2008.
The vine mealybug, Planococcus ficus (Signoret), is a severe pest of grapevine in many grape and wine producing countries around the world. It is renowned not only for the considerable damage it infers to grapevine of its own accord, but in particular for its role in transmitting deleterious viral diseases such as grapevine leafroll disease, Kober stem grooving, Shiraz disease and corky bark. Incidentally, it is an exceptionally tenacious antagonist of grapevine, being resistant to both chemical and biological control mechanisms. As a result, finding an effective strategy for P. ficus control has become a main priority of viticultural industries worldwide. Possible implementation of biotechnological approaches to pest management has resulted in a need for P. ficus genetic data - of which there are currently very little available. The transcribed genes of an organism can be captured in a cDNA library, and the sequences of the various transcripts can then be characterized. In this study altogether five cDNA libraries were constructed from the transcribed sequences of Planococcus ficus (Signoret). Instrumental to their construction was the identification of an RNA extraction protocol that provided large quantities of high quality RNA from mealybugs. The five cDNA libraries were the result of a set of modifications to the Creator™ SMART™ cDNA Library Construction Kit (used for Primary Library construction), and differed mainly with regards to range of insert sizes they contain. Whereas an abundance of short fragments were found in the Primary Library (42% of screened inserts 60.5 kb, and 20% >1 kb), the Fractionated Libraries contained inserts of specific size ranges that were more-or-less equally represented. The broadest size range was found in Fractionated Library 4, for which a uniform distribution over the range 0.25 kb - 4 kb was observed. Average insert sizes of Fractionated Libraries 1 to 4 were estimated at 0.25 kb, 0.5 kb, 1 kb and 2 kb respectively. These results demonstrated the importance of using a protocol designed to circumvent the bias towards incorporation of shorter transcripts in cDNA libraries. Although the libraries were not exhaustively analyzed, the outcome of a pilot investigation indicated that 41% of the submitted sequences had matches in the non-redundant database of the National Center for Biotechnology Information (NCBI, E-value 6 10-5), and that approximately 82% of these were of insect origin. Moreover, two potential targets for an RNAi-mediated approach to P. ficus pest control were identified. With one exception, these sequences seemed to be unique to arthropods. Future research needs to investigate the efficiency by which these sequences are able to constrain P. ficus proliferation, and their suitability for grapevine transformation.
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Holm, Kora. "Construction of a cDNA library for the vine mealybug, Planococcus ficus (Signoret) /." Link to the online version, 2008. http://hdl.handle.net/10019/1884.

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Builes, Janette Cristina. "Construction and screening of A cDNA library for the C3 gene(s) of the Nurse Shark (Ginglymostoma Cirratum)." FIU Digital Commons, 2001. http://digitalcommons.fiu.edu/etd/1940.

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Mammalian C3 is a complement protein which consists of an α chain (125kDa) and β chain (75kDa) held together by a disulfide bond. The a chain contains a conserved thiolester site which provides the molecule with opsonic properties. The protein is synthesized as a single pro-C3 molecule which is post-translationally modified. C3 genes have been identified in organisms from different phyla, however, the shark C3 gene remains to be cloned. Sequence data from the shark will contribute to understanding further the evolution of this key protein. To obtain additional sequence data for shark C3 genes a cDNA library was constructed and screened with a DIG-labeled C3 probe. Fifty clones were isolated and sequenced. Analysis identified four sequences that yielded positive alignments with C3 of a variety of organisms including human C3. Deduced amino acid sequence analysis confirmed a β/α cut site (RRRR), the CR3 and properdin binding sites, the catalytic histidine, and the reactive thiolester sequence. In the shark there are at least two C3-like genes as the gene sequence obtained is distinct from that previously described.
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Ponza, Pattareeya, and pattareeya pon@biotec or th. "Molecular markers of ecotoxicological interest in the rainbowfish Melanotaenia fluviatilis." RMIT University. Applied Science, 2007. http://adt.lib.rmit.edu.au/adt/public/adt-VIT20080102.121231.

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The Crimson-spotted rainbowfish (Melanotaenia fluviatilis) from the Murray-Darling basin of Australia is a common indicator species in Australian ecotoxicology. Biochemical changes have been investigated in this species, but not molecular markers of ecotoxicological interest. In this study genes of M. fluviatilis were isolated using a cDNA library and sequences analysed. Of 345 randomly selected clones, 94 shared similarity with 26 different genes in other organisms in public databases. Amongst these, reproductive genes coding for vitellogenin, retinol binding protein, sialyltransferase and zona pellucida protein were considered of interest in ecotoxicology. The vitellogenin gene was selected for study as it has been widely used as a molecular marker of exposure to 17â-estradiol (E2) in teleosts. Gene expression was examined via northern blot, RT-PCR and Real-Time PCR relative to the housekeeping gene (18S rRNA). The expression of vitellogenin mRNA was observed a t 12 hours post-exposure, peaked at 48 hours according to northern blot analysis; and cleared within 4 days, partly consistent with RT-PCR. However, Real-time PCR yielded an inconclusive result, probably due to differences between pooled and individual samples. Vitellogenin in blood plasma was confirmed by western blot, found to be significantly increased and retained in the plasma in fish treated with E2 compared to controls. It was concluded that vitellogenin mRNA is a molecular marker of exposure to 17â-estradiol in the rainbowfish, and could potentially be used as a marker of exposure to environmental estrogenic chemicals. Further investigations of the expression of genes in the cDNA library, could establish other molecular markers of ecotoxicological interest in M. fluviatilis.
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Soliman, Hatem Mohamed Touhan. "Construction and Screening of an Expression cDNA Library from the Triactinomyxon Spores of Myxobolus cerebralis, the causative agent of Salmonid Whirling Diseases." Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-39819.

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Soliman, Hatem Mohamed Touhan [Verfasser], and Mansour El [Akademischer Betreuer] Matbouli. "Construction and Screening of an Expression cDNA Library from the Triactinomyxon Spores of Myxobolus cerebralis, the causative agent of Salmonid Whirling Diseases / Hatem Mohamed Touhan Soliman. Betreuer: Mansour El-Matbouli." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2005. http://d-nb.info/1108093574/34.

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Hamdi, Saïd. "Regulation genetique et biochimique de l'auxine dans les souches fertiles et steriles de mercurialis annua : construction d'une banque de cdnas specifiques de la sterilite male." Orléans, 1987. http://www.theses.fr/1987ORLE2037.

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Huang, Zhen-Ming, and 黃震銘. "Construction of trichoderma koningii G39 cDNA library." Thesis, 1987. http://ndltd.ncl.edu.tw/handle/39237178009948159017.

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Zhang, Zhi Rui, and 張芝瑞. "Construction of mung bean cDNA library and cloning of B-galactosidase cDNA." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/29515359133940517941.

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Su, Yu-liang, and 蘇育良. "Construction and analysis of high reproductive porcine oocyte cDNA library." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/23810386909378501295.

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碩士
國立中山大學
生物醫學科學研究所
92
The progress of studies on genes concerning the development and differentiation of early swine embryos have been delayed by limited paucity material. In order to identify the porcine ESTs associates with promoting its breeding efficiency, a cDNA library and ESTs database from oocytes of high reproductive swine is established. Oocytes were obtained from Duroc pig by superovulation which was performed by Taiwan Livestock Research Institute, Council of Agriculture. Total RNA was isolated from 50 mature oocytes, reverse transcription is then performed, followed by PCR based amplification of the cDNA. The amplified cDNA size ranges from 0.4 to 5 kb. The derived cDNA were ligated to a pCR2.1 vector, and the library has complexities of about 5.26×104 independent clones. A total of 320 clones was picked and sequenced. By BLASTx analysis, among the 123 sequences, more than 43.07%（53/123） mitochondrial proteins are found, 56.91％（70/123） of the sequence were homologous to known transcripts from human, mouse, Drosophila. In nucleotide level analysis, 82.11％（101/123） matched with the mitochondrial, ribosome genes and 17.89％（22/123）matched with other homologous genes by BLASTn. PCR analysis of the oocyte library for specific genes revealed transcripts for genes including homologous genes（2 pairs highly abundance and 2 pairs low abundance genes）, housekeeping genes（ACTβ and G3PDH） and developmental genes（NEK2 and ZP1）. However, novel genes of swine are supposed to be the candidates for high productive phenotypes of swine. The library is a valuable resource for the isolation of clones representing genes active at the early stage. The ability to construct cDNA expression library from a few cells will allow gene expression analysis from oocyte biopsies and derived by nuclear transfer procedures.
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Book chapters on the topic "CDNA library construction"

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Schenk, Jörg A. "Two Hybrid cDNA Cloning." In Genetic Library Construction and Screening, 145–65. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_8.

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Curtis Bird, R., and Gin Wu. "Subtractive Hybridization and cDNA Cloning." In Genetic Library Construction and Screening, 97–125. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_6.

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Huang, Betty C. B., and Ying Luo. "Yeast One and Two Hybrid cDNA Cloning." In Genetic Library Construction and Screening, 167–85. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_9.

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Silverstein, Peter S., Shilpa J. Buch, and R. Curtis Bird. "Strategies for cDNA Cloning and Mapping RNA Transcripts." In Genetic Library Construction and Screening, 3–19. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_1.

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Jiang, Guifeng, and D. Jed Harrison. "mRNA Isolation for cDNA Library Construction on a Chip." In Micro Total Analysis Systems 2000, 537–40. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-017-2264-3_126.

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Snead, Marjory A., Michelle A. Alting-Mees, and Jay M. Short. "cDNA Library Construction for the Lambda ZAP®-Based Vectors." In Plant Virology Protocols, 255–67. Totowa, NJ: Humana Press, 1998. http://dx.doi.org/10.1385/0-89603-385-6:255.

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Zhu, York Y., Alex Chenchik, Roger Li, Florence Y. Hsieh, and Paul D. Siebert. "Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase." In Genetic Library Construction and Screening, 69–93. Berlin, Heidelberg: Springer Berlin Heidelberg, 2002. http://dx.doi.org/10.1007/978-3-642-56408-6_5.

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Singh, Rupesh Kumar, Weina Hou, and Gregory Franklin. "Construction of Hypericin Gland-Specific cDNA Library via Suppression Subtractive Hybridization." In Methods in Molecular Biology, 317–34. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3332-7_22.

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Liew, C. C., D. M. Hwang, R. X. Wang, S. H. Ng, A. Dempsey, D. H. Y. Wen, H. Ma, et al. "Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBest)." In Novel Methods in Molecular and Cellular Biochemistry of Muscle, 81–87. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-6353-2_8.

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Sahr, Tobias, and Carmen Buchrieser. "cDNA Library Construction for Next-Generation Sequencing to Determine the Transcriptional Landscape of Legionella pneumophila." In Methods in Molecular Biology, 555–66. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-161-5_34.

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Conference papers on the topic "CDNA library construction"

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Park, Sang-Eun. "cDNA library construction and gene screening ofHarmonia axyridis(Coleoptera: Coccinellidae) for gene functional analysis." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.112244.

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Kim, Jeong Hee. "cDNA library construction of 28-spotted potato ladybird,Henosepilachna vigintioctomaculata, for RNA interference application." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.110968.

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Pan, Baoping, Liyan Zhang, Weiwei Gao, and Xin Song. "Construction of cDNA Library and Preliminary Screening of Immune-Related Genes in Cyclina sinensis." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780015.

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Liu, Jiao, Shaoping Fu, Yuqiang Guo, Shuo Wang, Ruijun Duan, Ruimei Li, Yuan Yao, and Jianchun Guo. "Construction of a High-Quality Yeast One/two-hybrid cDNA Library from Cassava (Manihot Esculenta Crantz)." In International Conference on Biomedical and Biological Engineering. Paris, France: Atlantis Press, 2016. http://dx.doi.org/10.2991/bbe-16.2016.70.

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Huber, P., J. Dalmon, M. Laurent, G. Courtois, D. Thevenon, and G. Marguerie. "CHARACTERIZATION OFTHE 5’FLANKING REGION FOR THE HUMAN FIBRINOGEN β GENE." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1642889.

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Fibrinogen is coded by three separate genes located in a 50kb region of chromosome 4 and organized in a α - β - γ orientation with an inversion of the gene 3- A human genomic library was constructed using the EMBL4 phage and screened with cDNA probes coding for human fibrinogen Aα, Bβ and γ chains. Clones, covering the fibrinogen locus,were identified, and their organization was analyzed by means of hybridization and restriction mapping. Among these clones one recombinant phage containing the β gene and large 5’ and 3’ -flanking sequences was isolated.To identify the regulatory sequences Dpstream from the human β gene, a 1.5 kb fragment of the immediate 5’-flanking region was sequenced. The SI mapping experiments revealed three transcription initiation sites. PotentialTATA and CAAT sequences were identified upstream the initiation start points at the positions -21 and -58 from the first initiation start point.Comparison of this sequence with that previously reported for the same region upstream from the human γ gene revealed no significant homology which suggests that the potential promoting sequences of these genes are different. In contrast, comparison of the 5’flanking regions of human and rat β genes showed more than 80% homology for 142 bp upstream from the gene. This highly conserved region is a potential candidate for a regulatory sequence of the human β gene.To verify this activity, a β fibrinogen minigene was constructed by deletion of the internal part of the normal gene and including 3.4kb of the 5’flanking region and 1.4kb of the 3’flanking region. The minigene was transfected into HepG2, a human hepatoma cell line, to show whether the 5’flanking region of the human fibrinogen gene contains DNA sequences sufficient for efficient transcription in HepG2. Constructions of several parts of the sequenced 5’flanking region of the human β gene with the gene of the chloramphenical acetyl transferase have been also transfected in the HepG2 cells to determine the specificity of the gene expression and to localize the sequences controlling the transcription of the gene.
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Reports on the topic "CDNA library construction"

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Mitchell, S. C., D. Bocskai, and Y. Cao. Construction of genome-wide physical BAC contigs using mapped cDNA as probes: Toward an integrated BAC library resource for genome sequencing and analysis. Annual report, July 1995--January 1997. Office of Scientific and Technical Information (OSTI), December 1997. http://dx.doi.org/10.2172/639708.

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