Relevant bibliographies by topics / CD8

Academic literature on the topic 'CD8'

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Published: 4 June 2021
Last updated: 25 May 2024

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Journal articles on the topic "CD8"

1

Chatila, T. A., and R. S. Geha. "Phosphorylation of T cell membrane proteins by activators of protein kinase C." Journal of Immunology 140, no. 12 (June 15, 1988): 4308–14. http://dx.doi.org/10.4049/jimmunol.140.12.4308.

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Abstract Activation of the enzyme protein kinase C (PKC) plays an important role in T cell activation. We investigated the phosphorylation of CD2, CD3, CD4, CD5, CD7, CD8, CD28 (Tp44), CD43 (sialophorin, gp115), and LFA-1 after incubation of human PBMC with the (PKC) activator PMA. These proteins were chosen for their role in transmembrane signal transduction (CD2, CD3, CD5, CD28, CD43), cell-cell interaction and adhesion (CD2, CD4, CD8, and LFA-1), or involvement in immunodeficiency states (CD43, CD7). CD5, CD7, CD43, and the alpha-chain of LFA-1 were found to be constitutively phosphorylated. PMA induced rapid hyperphosphorylation of CD5, CD7, and CD43, but not of the LFA-1 alpha-chain, and induced the phosphorylation of CD3, CD4, CD8 and of the LFA-1 beta-chain. PMA did not cause the phosphorylation of CD2 and CD28. PMA-induced phosphorylation was partially inhibited by the PKC inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine dihydrochloride. Finally, the T cell activator Con A, which binds to the CD3/TCR complex was shown to induce a profile of protein phosphorylation similar to that observed with PMA. We conclude that PKC-mediated phosphorylation of T cell Ag may represent an important regulatory mechanism that governs the process of T cell activation.
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Зыблева, С. В., and С. Л. Зыблев. "Cluster Analysis of Leukocyte Subpopulations in Kidney Transplantation." Гематология. Трансфузиология. Восточная Европа, no. 2 (November 8, 2021): 168–75. http://dx.doi.org/10.34883/pi.2021.7.2.005.

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Цель. Выявить варианты иммунного реагирования у пациентов при трансплантации почки на основе кластерного анализа, характеризующие течение посттрансплантационного периода. Материалы и методы. Обследовано 104 реципиента почечного трансплантата с терминальной стадией хронической болезни почек, которым выполнена трансплантация аллогенной почки, а также 90 здоровых добровольцев, составивших группу сравнения. Оценены уровни лейкоцитов с использованием метода проточной цитометрии по безотмывочной технологии с использованием моноклональных антител (Beckman Coulter и BD, США) CD4 PС7, CD8 FITC, CD3 PС5.5, CD3 FITC, CD45 PerCP, CD19 APC, CD56+ CD16 PE, CD3 PC5.5, HLA-DR APC, CD38 PE, CD4 FITC, CD3 PC5.5, CD8 APC, CD69 PE, CD127 PЕ, CD25 АРС CD154 PЕ, CD3 APCAF750, IgD FITC, CD27 PC5.5, CD5 APC-AF750, CD40РЕ, CD86 РЕ, CD14 PС7, CD64 FITC, CD86 PЕ LIN PE, CD123 PC7, Anti-HLADR APC-AF750 в объемах, рекомендуемых фирмой-производителем. Результаты и обсуждение. В результате проведенного исследования разработана система оценки иммунного статуса реципиента почечного трансплантата, обеспечивающая персонифицированный мониторинг, анализ и прогнозирование течения посттрансплантационного периода. Описаны виды регуляторных клеточных сетей и их синергический потенциал при трансплантации почки. В основе толерогенного иммунологического комплекса у пациентов после трансплантации почки лежат межклеточные взаимодействия, имеющие иерархическую систему, основа которой представлена кооперацией клеток CD3+CD4+CD25+highCD127+low, CD3+CD4-CD8-, CD3+CD4+CD69+, CD3+CD16+CD56+, CD19+CD5+ и LIN-HLA-DR+CD11c-CD123+. Воснове гиперергического иммунологического комплекса при почечной аллотрансплантации лежат избыточно активированные компоненты иммунного ответа: CD3+CD8+CD69+, CD3+CD4+CD8+, CD3+CD8+CD38+, CD19+CD86+, CD19+IgD+CD27-, CD3-CD16+CD56+, CD3+CD38+, CD14+lowCD86+, и LIN-HLA-DR+CD11c+CD123клетки. Выводы. Выделенные иммунофенотипы позволят осуществить персонифицированный подход к диагностике и лечению пациентов с различными вариантами иммунного реагирования при трансплантации почки. При выявлении иммунофенотипа, соответствующего толерогенному варианту иммунного ответа, терапию пациента в отдаленном посттрансплантационном периоде возможно проводить с учетом низкого иммунологического риска и минимизацией иммуносупрессивной нагрузки. Purpose. To identify the variants of immune response in patients with kidney transplantation on the base of cluster analysis that characterize the course of the post-transplant period. Materials and methods. We examined 104 kidney transplant recipients with end-stage chronic kidney disease, who underwent allograft transplantation, as well as 90 healthy volunteers, who made up the comparison group. Their leukocyte levels were assessed using no-wash flow cytometry with monoclonal antibodies (Beckman Coulter and BD, USA) CD4 PС7, CD8 FITC, CD3 PС5.5, CD3 FITC, CD45 PerCP, CD19 APC, CD56+ CD16 PE, CD3 PC5.5, HLA-DR APC, CD38 PE, CD4 FITC, CD3 PC5.5, CD8 APC, CD69 PE, CD127 PЕ, CD25 АРС CD154 PЕ, CD3 APC-AF750, IgD FITC, CD27 PC5.5, CD5 APC-AF750, CD40РЕ, CD86 РЕ, CD14 PС7, CD64 FITC, CD86 PЕ LIN PE, CD123 PC7, Anti-HLADR APC-AF750 in the volumes recommended by the manufacturer. Results and discussion. As a result of the conducted study, the system for assessing the immune status of a kidney transplant recipient was developed, which provides personalized monitoring, analysis, and prediction of the course of the post-transplant period. The types of regulatory cellular networks and their synergistic potential in kidney transplantation are described. The tolerogenic immunological complex in patients after kidney transplantation is based on intercellular interactions that have a hierarchical system, the base of which is represented by the cooperation of CD3+CD4+CD25+highCD127+low, CD3+CD4-CD8-, CD3+CD4+CD69+, CD3+CD16+CD56+, CD19+CD5+ and LIN-HLA-DR+CD11c-CD123+ cells. The hyperergic immunological complex in renal allotransplantation is based on over-activated components of the immune response: CD3+CD8+CD69+, CD3+CD4+CD8+, CD3+CD8+CD38+, CD19+CD86+, CD19+IgD+CD27-, CD3-CD16+CD56+, CD3+CD38+, CD14+lowCD86+, and LIN-HLA-DR+CD11c+CD123- cells. Conclusion. The detected immunotypes will let to implement the personalized approach to the diagnostics and treatment of patients with various types of immune response in kidney transplantation. If an immunophenotype corresponding to a tolerogenic variant of the immune response is identified, the patient’s therapy in the long-term post-transplant period can be carried out taking into account the low immunological risk and minimizing the immunosuppressive load.
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Zybleva, S. V., and S. L. Zyblev. "Immunological cluster complexes in kidney transplantation." Medical Immunology (Russia) 24, no. 1 (March 10, 2022): 69–80. http://dx.doi.org/10.15789/1563-0625-icc-2212.

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Laboratory tests are significant for the detection of immunopathological disorders in kidney transplantation. As a rule, the choice of tests is carried out individually and is based on the clinical characteristics and the presumptive diagnosis. Most often, in patients after kidney transplantation, atypical and not always standard changes in immunological parameters are observed, which is associated with a combination of many factors leading to different immune responses. All this served as the basis for typing immunological parameters in renal allograft recipients using one of the methods of system analysis – cluster analysis. Kidney transplantation was performed in 104 recipients. Immunological examination was performed on the 360th day after the surgery. The following groups of recipients were identified: KTR1 – with primary graft function on the 7th day and satisfactory graft function within a year, KTR2 – with renal graft dysfunction on the 7th day and within a year. By means of cluster analysis, immunotypes of regulatory complexes were detected and characterized in various courses of the post-transplant period. To assess the immune response in allogeneic kidney transplantation, a set of immune cells with a phenotype should be determined: CD3+CD4+CD25+highCD127+low, CD3+CD4-CD8-, CD3+CD4+CD69+, CD3+CD16+CD56+, CD19+CD5+, LIN-HLA-DR+CD11c-CD123+, CD3+CD8+CD69+, CD3+CD4+CD8+, CD3+CD8+CD38+, CD19+CD86+, CD19+IgD+CD27-, CD3-CD16+CD56+, CD3+CD38+, CD14+lowCD86+, LIN-HLA-DR+CD11c+CD123-. According to our data, the immunological cellular composition of the central point of clustering of the tolerogenic immunological complex is represented by regulatory CD3+CD4+CD25+highCD127+low and double-negative CD3+CD4-CD8-T lymphocytes. The composition of the central point of clustering of the hyperergic immunological complex is represented by the cooperation of CD3+CD8+CD69+ and CD3+CD4+CD8+ cells. The structure of the tolerogenic immune response in patients after kidney transplantation is based on intercellular interactions, which has a hierarchical system, the basis of which is represented by the cooperation of regulatory cells CD3+CD4+CD25+highCD127+low, CD3+CD4-CD8-, CD3+CD4+CD69+, CD3+CD16+CD56+, CD19+CD5+, LIN-HLA-DR+CD11c-CD123+. The hyperergic variant of the immune response in renal allograft transplantation is based on excessive activation of the following links of the immune response: CD3+CD8+CD38+, CD19+CD86+, CD3+CD38+, LIN-HLADR+CD11c+CD123-, CD19+IgD+CD27-, CD3-CD16+CD56+, CD3+CD8+CD69+ and CD14+lowCD86+. The detected immunotypes will make it possible to implement a personalized approach to the diagnosis and treatment of patients with various types of immune response in kidney transplantation.
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Tjønnfjord, G. E., O. P. Veiby, R. Steen, and T. Egeland. "T lymphocyte differentiation in vitro from adult human prethymic CD34+ bone marrow cells." Journal of Experimental Medicine 177, no. 6 (June 1, 1993): 1531–39. http://dx.doi.org/10.1084/jem.177.6.1531.

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Pluripotent lymphohematopoietic stem cells are probably confined to bone marrow cells expressing CD34 surface molecules. To investigate the capacity of adult human CD34+ bone marrow cells to differentiate along the T lymphoid lineage, we plated purified CD34+ cells from healthy adults in liquid culture on adherent thymic stromal cells prepared from HLA- or blood group-mismatched postnatal thymic tissue. We show that purified CD34+CD3-CD4-CD8- bone marrow cells contained progenitors with the ability to differentiate into CD4+ and CD8+ T lymphocytes expressing surface (s)CD3 and T cell receptor alpha/beta in vitro. These progenitors were found in the CD34+CD2+sCD3-CD4-CD8-, CD34+CD7+sCD3-CD4-CD8-, and CD34+CD2+CD7+sCD3-CD4-CD8-, as well as in the CD34+CD2-sCD3-CD4-CD8-, CD34+CD7-sCD3-CD4-CD8-, and CD34+CD2-CD7-sCD3-CD4-CD8- subsets, indicating that T lymphocyte progenitors sensitive to signals mediated by thymic stroma in vitro are not restricted to CD34+ cells already coexpressing early T lymphocyte-associated markers. Finally, we show that T lymphopoiesis was enhanced by c-kit ligand.
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Damle, N. K., and L. V. Doyle. "Stimulation via the CD3 and CD28 molecules induces responsiveness to IL-4 in CD4+CD29+CD45R- memory T lymphocytes." Journal of Immunology 143, no. 6 (September 15, 1989): 1761–67. http://dx.doi.org/10.4049/jimmunol.143.6.1761.

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Abstract Although both IL-2 and IL-4 can promote the growth of activated T cells, IL-4 appears to selectively promote the growth of those helper/inducer and cytolytic T cells which have been activated via their CD3/TCR complex. The present study examines the participation of CD28 and certain other T cell-surface molecules in inducing T cell responsiveness to IL-4. Purified small high density T cells were cultured in the absence of accessory cells with various soluble anti-human T cell mAb with or without soluble anti-CD3 mAb and their responsiveness to IL-4 was studied. None of the soluble anti-T cell mAb alone was able to induce T cell proliferation in response to IL-4. A combination of soluble anti-CD3 with anti-CD28 mAb but not with mAb directed at the CD2, CD5, CD7, CD11a/CD18, or class I MHC molecules induced T cell proliferation in response to IL-4. Anti-CD2 and anti-CD5 mAb enhanced and anti-CD18 mAb inhibited this anti-CD3 + anti-CD28 mAb-induced T cell response to IL-4. In addition, anti-CD2 in combination with anti-CD3 and anti-CD28 mAb induced modest levels of T cell proliferation even in the absence of exogenous cytokines. IL-1, IL-6, and TNF were each unable to replace either anti-CD3 or anti-CD28 mAb in the induction of T cell responsiveness to IL-4, but both IL-1 and TNF enhanced this response. The anti-CD3 + anti-CD28 mAb-induced response to IL-4 was exhibited only by cells within the CD4+CD29+CD45R- memory T subpopulation, and not by CD8+ or CD4+CD45R+ naive T cells. When individually cross-linked with goat anti-mouse IgG antibody immobilized on plastic surface, only anti-CD3 and anti-CD28 mAb were able to induce T cell proliferation. These results indicate that the CD3 and CD28 molecules play a crucial role in inducing T cell responsiveness to IL-4 and that the CD2, CD5, and CD11a/CD18 molecules influence this process.
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Haynes, B. F., and C. S. Heinly. "Early human T cell development: analysis of the human thymus at the time of initial entry of hematopoietic stem cells into the fetal thymic microenvironment." Journal of Experimental Medicine 181, no. 4 (April 1, 1995): 1445–58. http://dx.doi.org/10.1084/jem.181.4.1445.

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To determine events that transpire during the earliest stages of human T cell development, we have studied fetal tissues before (7 wk), during (8.2 wk), and after (9.5 wk to birth) colonization of the fetal thymic rudiment with hematopoietic stem cells. Calculation of the approximate volumes of the 7- and 8.2-wk thymuses revealed a 35-fold increase in thymic volumes during this time, with 7-wk thymus height of 160 microM and volume of 0.008 mm3, and 8.2-wk thymus height of 1044 microM and volume of 0.296 mm3. Human thymocytes in the 8.2-wk thymus were CD4+ CD8 alpha+ and cytoplasmic CD3 epsilon+ cCD3 delta+ CD8 beta- and CD3 zetta-. Only 5% of 8-wk thymocytes were T cell receptor (TCR)-beta+, < 0.1% were TCR-gamma+, and none reacted with monoclonal antibodies against TCR-delta. During the first 16 wk of gestation, we observed developmentally regulated expression of CD2 and CD8 beta (appearing at 9.5 wk), CD1a,b, and c molecules (CD1b, then CD1c, then CD1a), TCR molecules (TCR-beta, then TCR-delta), CD45RA and CD45RO isoforms, CD28 (10 wk), CD3 zeta (12-13 wk), and CD6 (12,75 wk). Whereas CD2 was not expressed at the time of initiation of thymic lymphopoiesis, a second CD58 ligand, CD48, was expressed at 8.2 wk, suggesting a role for CD48 early in thymic development. Taken together, these data define sequential phenotypic and morphologic changes that occur in human thymus coincident with thymus colonization by hematopoietic stem cells and provide insight into the molecules that are involved in the earliest stages of human T cell development.
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Abbott, Daniel, Steven Kroft, Maria Hintzke, Luis Carrillo-Polanco, Ashley Cunningham, John Astle, Vasiliki Leventaki, and Alexandra Harrington. "Immunophenotypic Analysis of Peripheral T-Cell Lymphomas: A Single-Center Retrospective Review of Flow Cytometric Analysis." American Journal of Clinical Pathology 152, Supplement_1 (September 11, 2019): S109. http://dx.doi.org/10.1093/ajcp/aqz121.012.

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Abstract Background Peripheral T-cell lymphomas (PTCLs) are heterogenous, mature T-cell neoplasms that are a diagnostic challenge, requiring a combination of morphologic assessment and ancillary studies. Flow cytometry (FC) is a tool used routinely in lymphoma diagnosis; however, most analyses are limited to B-cell evaluation and pathologists generally lack experience evaluating for PTCL. We aimed to describe the immunophenotypic aberrancies observed by FC in PTCL. Design PTCLs with FC were collected, excluding primary leukemic processes. Four- and eight-color FC data were reanalyzed with the following antigens (when available): CD2, CD3, CD4, CD5, CD7, CD8, CD30, CD45, CD45RO, CD56, and CD57. Lymphoma cells were compared to normal T cells and an isotype control. Antigen expression was defined as >20%. Results Thirty-eight cases were analyzed (29 males, 9 females, 6-86 years, median 62 years), including 29 PTCLs NOS, 4 angioimmunoblastic T-cell lymphomas (AITLs), 3 anaplastic large cell lymphomas, 1 δγ-TCL, and 1 hepatosplenic TCL from 15 bone marrows, 14 lymph nodes, 6 bloods, 2 fluids, and 1 skin. Twenty cases were CD4+, 4 were CD8+, 3 were dual +, and 10 were dual –. Thirty-seven cases (97%) showed global aberrant antigen patterns, median 4 aberrancies/case (1-8). Lymphoma cells accounted for 0.07% to 68% (median 2.6%) of total events. Aberrant CD7 expression was present in 34 of 38 (89%) and was underexpressed in 22 of 34 (65%). CD3 and CD5 were aberrant in 79% of cases each, with two-thirds showing underexpression. CD2 and CD45RO were aberrant in two-thirds of PTCLs, with overexpression in 61% and 92% of those cases, respectively. One AITL showed no aberrancies. Conclusions Nearly all PTCLs show immunophenotypic aberrancy compared to normal T cells. Most commonly, PTCL showed aberrant underexpression of CD7, CD3, and CD5 and overexpression of CD2 and CD45RO. Our data support FC panels with CD2, CD3, CD4, CD5, CD7, CD8, and CD45RO to optimize recovery of aberrant T cells.
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Akhmatova, E. A., E. V. Sorokina, I. Zh IShubina, E. A. Kurbatova, V. N. Stolpnikova, E. O. Kalinichenko, I. V. Bisheva, and S. A. Skhodova. "Innate immunity cells in a model of acute psoriasis-like inflammation in mice." Russian Journal of Biotherapy 22, no. 4 (November 22, 2023): 43–51. http://dx.doi.org/10.17650/1726-9784-2023-22-4-43-51.

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Background. Experimental animal models of psoriasis helped to clarify the functions of inflammatory mediators, to reveal the contribution of innate or adaptive immune mechanisms, keratinocytes to the development and maintenance of inflammation in psoriasis.Aim. To study the subpopulation composition of immune cells of blood, skin, lymphoid organs and compare two methods of isolation of cells from the skin.Materials and methods. The study included 46 mice of the C57BL / 6 line, which were divided into 2 groups: experimental (n = 24) to reproduce a model of acute psoriasis-like dermatitis using imiquimod cream 5 % (62.5 mg / cm2 / day / mouse, 7 days) and control (n = 22). The severity of skin inflammation was assessed on a point scale. On the 7th day, the skin, spleen, lymph nodes, and thymus were examined. To isolate cells from the skin, the method of spontaneous migration and enzymatic dissociation using collagenase was used. The assessment of the subpopulation structure of mononuclear cells (MNCs) was carried out by flow cytometry using monoclonal antibodies against the corresponding antigens (CD3, CD4, CD5, CD8, MHC class II, TCRyδ, CD38, CD80, CD83, CD86, TLR2). Statistical processing was carried out using the winMDI 2.8 software package.Results. It has been shown that both methods of isolation of skin cells are applicable for immunophenotyping of γδ T-lymphocytes, CD86+, CD83+, CD83+CD86+ dendritic cells. A decrease in TLR2 expression on blood cells and an increase in lymph node and skin cells were revealed. There was a marked increase in the number of CD38+ in the lymph nodes, thymus, and an increase in γδ T-lymphocytes in the lymph nodes and blood. The infiltration of γδ T-lymphocytes, CD8+ is shown in the skin and CD38+ cells.Conclusion. Acute psoriasis-like inflammation of mice was accompanied by an increase in the number of γδ T cells in the blood, lymph nodes and skin. Infiltration of the skin by CD8+ and CD38+ cells was observed. Both methods of cell isolation – the method of spontaneous migration and the method of enzymatic dissociation proved to be applicable for further immunophenotyping.
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Terstappen, LW, S. Huang, and LJ Picker. "Flow cytometric assessment of human T-cell differentiation in thymus and bone marrow." Blood 79, no. 3 (February 1, 1992): 666–77. http://dx.doi.org/10.1182/blood.v79.3.666.bloodjournal793666.

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Using multidimensional flow cytometry we have defined and quantified the human T-cell differentiation pathway, focusing on those events occurring among the most immature thymocytes and putative bone marrow (BM) T-precursors. Early thymocytes were found to express the CD34 antigen and consisted of a mean 1.2% of cells within human pediatric (n = 9) and 2.0% in fetal thymi (n = 4). All CD34+ thymocytes were atypical blast by morphology, expressed intracytoplasmatic, but not cell surface, CD3, and were cell surface CD2+, CD5+, CD7+, CD38+, CD45+, CD45RA+, CD49d+, and LECAM-1(Leu8)high. CD34high thymocytes lacked surface expression of CD4 and CD8, but as CD34 expression diminished there was a coordinate increase in CD4 levels, followed by the appearance of CD8. The expression of CD1 and CD10 also increased concomitant with the loss of CD34, whereas expression of LECAM-1 diminished with CD34 downregulation. The differential expression of these antigens on early thymocytes (as well as the number of thymocytes displaying these patterns) was highly reproducible among the nine pediatric and four fetal specimens examined, suggesting a precise, stereotyped regulation of early differentiation events. Cell populations with antigen expression patterns suggestive of pluripotent stem cell (CD34high, CD38-), or non-T-lineage committed stem cells (CD34+, CD33+ or CD34+, CD19+) were not identified in either fetal or pediatric thymi (sensitivity = 1/10(4)). The presence of cells with the antigenic profile of the earliest CD34+ thymocytes was explored in human BM. Putative BM T-cell precursors with the appropriate phenotype (CD34+, CD7+, CD5+, CD2+, LECAM-1high) were readily identified in fetal specimens (constituting +/- 2% of the CD34+ population), but could not be reliably detected in adults. In contrast with thymi, only 13% of these cells expressed cytoplasmatic CD3, suggesting the presence of the immediate precursor of the putative prothymocyte population. This was further supported by the detection of CD34bright, CD7+, CD2-, CD5-, LECAM-1moderate cells in fetal specimens. Our results document the flow of cell surface differentiation during T-lymphopoiesis and suggest that T-lineage features are first acquired in the BM. The ability to reproducibly identify and isolate T-cell precursor populations of precisely defined maturational stage in marrow and thymus by multiparameter flow cytometry will facilitate characterization of the molecular events controlling T-lineage differentiation.
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Raimondi, SC, FG Behm, PK Roberson, CH Pui, GK Rivera, SB Murphy, and DL Williams. "Cytogenetics of childhood T-cell leukemia." Blood 72, no. 5 (November 1, 1988): 1560–66. http://dx.doi.org/10.1182/blood.v72.5.1560.1560.

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Abstract The karyotypes of 57 cases of childhood T-cell acute lymphoblastic leukemia (ALL) were analyzed to establish the cytogenetic profile in this disease. Three questions were of particular interest. Do the chromosomal changes in T-cell ALL preferentially affect bands where genes encoding the T-cell receptor for antigen (TCR) have been mapped? Do alterations involving the TCR gene regions appear with any notable frequency in B-progenitor ALL? Do chromosomal abnormalities in this disease relate to stage of T-cell ontogeny? A relatively high proportion of cases (65%) had a pseudodiploid karyotype at presentation, the majority (58%) characterized by a translocation. The overall frequency of translocations was 44%, comparable to that among all banded cases of ALL seen in our laboratory. Hypodiploidy and hyperdiploidy were exceedingly rare (only four of 57 cases); 16 cases (28%) had apparently normal karyotypes. In half the cases with a translocation (14 of 24), the breakpoints were in regions to which the alpha and beta chain TCR genes have been mapped. Chromosomal breakpoints that were consistently observed in the vicinity of TCR gene loci were 7q32-q36 (TCR beta chain; n = 8), 14q11-q13 (TCR alpha chain; n = 6); other frequent breakpoints were 9p13-pter (n = 8) and 6q15-qter (n = 9). Chromosomal alterations occurred near TCR gene loci significantly more often in T-cell cases than in a comparison group of 335 patients with B-cell precursor ALL (26% v 1.5%, P = .0001). Stage I thymocyte development (CD7+, CD2+, CD5+, CD1-, CD3-, CD4-, CD8-) was noted in 23 cases, stage II (CD7+, CD2+, CD5+, CD1+, CD3-, CD4 +/-, CD8 +/-) in 25 cases, and stage III (CD9+, CD2+, CD1-, CD5+, CD3+, and either CD4+ or CD8+) in nine cases. The only statistically significant associations between cytogenetic findings and T-cell ontogeny were a higher frequency of normal karyotypes in cases with stage I thymocytes, and of pseudodiploidy in stage II cases. There was no apparent relationship between particular translocations and level of thymocyte maturation. Our findings indicate that most children with T-cell ALL have pseudodiploid karyotypes, although a surprisingly high percentage lack demonstrable abnormal clones. Specific chromosomal changes do not appear to be related to discrete stages of T-cell ontogeny as defined in this study, but they occur preferentially in bands containing TCR genes.
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Dissertations / Theses on the topic "CD8"

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Thomas, Ian James. "Investigation of the differential effects of CD80 and CD86 costimulation on CD8 T cells." Thesis, University of Bristol, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.424069.

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Eichelbauer, Dirk. "In-vitro-Untersuchungen zur Stimulation von humanen TZR-[alpha]/[beta]+-CD4-CD8-doppeltnegativen [TZR-alpha-beta-CD4-CD8-doppeltnegativen] T-Lymphozyten." [S.l. : s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=970313373.

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Cauchy, Pierre. "Rôle et contexte transcriptionnel du facteur de transcription Ets1 au cours transition CD4- CD8- à CD4+ CD8+ de la tymopoïèse αβ." Thesis, Aix-Marseille 2, 2010. http://www.theses.fr/2010AIX22135.

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ETS1 est un facteur de transcription (FT) spécifique transposé dans les leucémies aigües. Le rôle essentiel d'ETS1 a été décrit au cours de l'hématopoïèse, plus particulièrement dans la différenciation lymphocytaire T. Son expression temporelle coordonnée participe au contrôle des transitions du stade double négatif (DN) CD4-/CD8- au stade double positif (DP) CD4+/CD8+jusqu'au stade simple positif (SP) CD4+ ou CD8+. Au cours de l'ontogenèse T, ETS1 transactive notamment l'expression des chaînes β et α du récepteur des cellules T (TCR). Nous avons criblé à grande échelle les cibles d'ETS1 aux stades DN et DP en ChIP-Seq, ainsi que desmarques histone et de l'ARN polymérase II (Pol II). Afin de faciliter nos analyses bioinformatiques, nous avons développé deux logiciels, CoCAS et AmaMineReg, qui permettent d'identifier plus facilement les cibles à partir de données brutes et de discriminer les vrais des faux positifs. Nous avons trouvé 5900 cibles en DN et 3400 en DP, principalement intergéniques dont 2000 sont communes, non caractérisées et correspondent aux gènes induits par la réponse immédiate à la signalisation TCR. Parmi les cibles différentiellement exprimées entre les deux stades, ETS1 active les gènes thymus-spécifiques et réprime les gènes hématopoïétiques non T spécifiques,en fonction de la co-occurrence avec le motif RUNX1. Nous avons également caractérisé très clairement le site de fixation en conditions natives, qui se révèle être CTTCCT.De plus, ETS1 co-localise avec des marques chromatines permissives aux régions inter- et intragéniques,caractérisées par un contenu GC, densité de motifs de fixation de FT (SFFT) et conservation inter-espèces accrus
ETS1 is a specific transcription factor (TF) transposed in acute leukemias. key role of ETS1 wasdescribed during hematopoiesis, especially in T lymphocyte differentiation. Its temporal expression participates in the coordinated control of phase transitions from the CD4-/CD8-double negative (DN) stage to CD4+/CD8+ double positive (DP) up to CD4 or CD8 single positivestage (SP). During ontogenesis T ETS1 notably transactivates the expression of the alpha and beta chains of the T-Cell receptor (TCR). We performed genome-wide screening of ETS1 at both DN and DP stages via ChIP-Seq, as well as histone hallmarks and RNA polymerase II (PolII). To facilitate computational analysis we developed two new software suites, and COCASAmaMineReg, which allow easier identification of targets from raw data and to discriminate between true and false positives. We found 5900 targets in 3400 in DN and DP, mostly intergenic, out of which 2000 are common, and correspond to uncharacterized genes induced bythe immediate response to TCR signaling. Among targets differentially expressed between thetwo stages, Ets1 activates thymus-specific genes and represses non T-specific haematopoietic genes depending on the co-occurrence with the RUNX1 motif. We also very clearly characterized the binding site in native conditions, which proved to be CTTCCT. Furthermore, Ets1 colocalizes with permissive chromatin marks in inter-and intra-genic regions, characterized byincreased GC content, TF binding motifs (TFBS) density as well as inter-species conservation
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Pinheiro, CatiÃssia Dantas. "CÃlulas CD3+, CD4+, CD8+, CD3-CD16+CD56+ e CD19+ em sangue perifÃrico de pacientes com hansenÃase e indivÃduos saudÃveis." Universidade Federal do CearÃ, 2013. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=16323.

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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
A hansenÃase à uma doenÃa granulomatosa, infecto-contagiosa causada pelo Mycobacterium leprae. Trata-se de uma infecÃÃo crÃnica com amplo espectro de respostas imunes celulares em humanos. Possui alto poder infectante e baixo poder patogÃnico. Este estudo tem como objetivo quantificar e comparar leucÃcitos e subpopulaÃÃes de linfÃcitos T totais (CD3+), T auxiliares (CD3+CD4+), T citotÃxicos (CD3+CD8+), B (CD19+) e NK (CD3-CD16+CD56+) em sangue perifÃrico de indivÃduos com hansenÃase e controles saudÃveis. Os pacientes foram provenientes do Centro de Dermatologia D. LibÃnia, Fortaleza-CE, Brasil. A determinaÃÃo do nÃmero de linfÃcitos em cada subpopulaÃÃo foi realizada por citometria de fluxo. A anÃlise estatÃstica foi realizada pelo programa GraphPad Prism 5.0 para Windows com significÃncia estabelecida para valores de p<0,05. à um estudo do tipo caso controle de carÃter observacional, realizado a partir da anÃlise do sangue perifÃrico de indivÃduos com diagnÃstico de hansenÃase e de indivÃduos saudÃveis. A populaÃÃo de pacientes com hansenÃase, sem tratamento foi composta de 15 pessoas. A populaÃÃo de controles saudÃveis foi composta por 29 pessoas. As mÃdias das contagens de LinfÃcitos NK (CD3-CD16+CD56+) no grupo de pacientes com hansenÃase e nos controles saudÃveis, dadas em cÃlulas/mm3, foram, 147(Â113,4) e 378,1 (Â231,7) respectivamente, p = 0,0008. As mÃdias das contagens de LinfÃcitos B (CD19+) no grupo de pacientes com hansenÃase e nos controles saudÃveis, dadas em cÃlulas/mm3, foram, 233,3 (Â85,89) e 115,3 (Â53,01) , respectivamente, p < 0,0001. NÃo foram encontradas diferenÃas estatÃsticas significantes entre as amostras de leucÃcitos, de linfÃcitos T CD3+, linfÃcitos T CD4+ e linfÃcitos T CD8+. Os dados do presente estudo sinalizam que as cÃlulas NK parecem desempenhar papel de relevÃncia na resposta ao M. leprae. O linfÃcito B jà ocupa papel de destaque na resposta imunolÃgica ao M. leprae, sobretudo nas formas lepromatosas, e este estudo reforÃa a importÃncia destas cÃlulas.
Leprosy is an infectious and granulomatous disease caused by Mycobacterium leprae. The aim of this study was to quantify and compare levels of leucocytes and lymphocyte subpopulations (CD3+, CD3+CD4+, CD3+CD8+, CD19+, CD3-CD16+CD56+) in peripheral blood of patients with leprosy and healthy controls. Patients were followed at Centro de Dermatologia D. LibÃnia, Fortaleza-CE, Brasil. Flow cytometry was used to determine numbers of lymphocytes. Statistical analisys was done with GraphPad Prism 5.0 software for windows. P values under 0.05 were considered siginificant.This was an observational case-control study. Fifteen leprosy patients without treatment were evaluated and 29 healthy individuals were included in control group. NK cells (CD3-CD16+CD56+) mean in leprosy patients was 147(Â113,4) and in controls was 378,1 (Â231,7). Comparisson stablished a p value of 0.0008. B lymphocytes (CD19+) mean in leprosy patients was 233,3 (Â85,89) and in controls was 115,3 (Â53,01), with p < 0,0001 . No differences were observed in CD3+ T lymphocytes, CD4+ T lymphocytes and CD8+ T lymphocytes. This study suggests that NK cells may play a role in innate response to M. leprae.
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Pinheiro, Catiússia Dantas. "Células CD3+, CD4+, CD8+, CD3-CD16+CD56+ e CD19+ em sangue periférico de pacientes com hanseníase e indivíduos saudáveis." reponame:Repositório Institucional da UFC, 2013. http://www.repositorio.ufc.br/handle/riufc/15425.

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PINHEIRO, Catiússia Dantas. Células CD3+, CD4+, CD8+, CD3-CD16+CD56+ e CD19+ em sangue periférico de pacientes com hanseníase e indivíduos saudáveis. 2013. 65 f. Dissertação (Mestrado em Patologia) - Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, 2013.
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Leprosy is an infectious and granulomatous disease caused by Mycobacterium leprae. The aim of this study was to quantify and compare levels of leucocytes and lymphocyte subpopulations (CD3+, CD3+CD4+, CD3+CD8+, CD19+, CD3-CD16+CD56+) in peripheral blood of patients with leprosy and healthy controls. Patients were followed at Centro de Dermatologia D. Libânia, Fortaleza-CE, Brasil. Flow cytometry was used to determine numbers of lymphocytes. Statistical analisys was done with GraphPad Prism 5.0 software for windows. P values under 0.05 were considered siginificant.This was an observational case-control study. Fifteen leprosy patients without treatment were evaluated and 29 healthy individuals were included in control group. NK cells (CD3-CD16+CD56+) mean in leprosy patients was 147(±113,4) and in controls was 378,1 (±231,7). Comparisson stablished a p value of 0.0008. B lymphocytes (CD19+) mean in leprosy patients was 233,3 (±85,89) and in controls was 115,3 (±53,01), with p < 0,0001 . No differences were observed in CD3+ T lymphocytes, CD4+ T lymphocytes and CD8+ T lymphocytes. This study suggests that NK cells may play a role in innate response to M. leprae.
A hanseníase é uma doença granulomatosa, infecto-contagiosa causada pelo Mycobacterium leprae. Trata-se de uma infecção crônica com amplo espectro de respostas imunes celulares em humanos. Possui alto poder infectante e baixo poder patogênico. Este estudo tem como objetivo quantificar e comparar leucócitos e subpopulações de linfócitos T totais (CD3+), T auxiliares (CD3+CD4+), T citotóxicos (CD3+CD8+), B (CD19+) e NK (CD3-CD16+CD56+) em sangue periférico de indivíduos com hanseníase e controles saudáveis. Os pacientes foram provenientes do Centro de Dermatologia D. Libânia, Fortaleza-CE, Brasil. A determinação do número de linfócitos em cada subpopulação foi realizada por citometria de fluxo. A análise estatística foi realizada pelo programa GraphPad Prism 5.0 para Windows com significância estabelecida para valores de p<0,05. É um estudo do tipo caso controle de caráter observacional, realizado a partir da análise do sangue periférico de indivíduos com diagnóstico de hanseníase e de indivíduos saudáveis. A população de pacientes com hanseníase, sem tratamento foi composta de 15 pessoas. A população de controles saudáveis foi composta por 29 pessoas. As médias das contagens de Linfócitos NK (CD3-CD16+CD56+) no grupo de pacientes com hanseníase e nos controles saudáveis, dadas em células/mm3, foram, 147(±113,4) e 378,1 (±231,7) respectivamente, p = 0,0008. As médias das contagens de Linfócitos B (CD19+) no grupo de pacientes com hanseníase e nos controles saudáveis, dadas em células/mm3, foram, 233,3 (±85,89) e 115,3 (±53,01) , respectivamente, p < 0,0001. Não foram encontradas diferenças estatísticas significantes entre as amostras de leucócitos, de linfócitos T CD3+, linfócitos T CD4+ e linfócitos T CD8+. Os dados do presente estudo sinalizam que as células NK parecem desempenhar papel de relevância na resposta ao M. leprae. O linfócito B já ocupa papel de destaque na resposta imunológica ao M. leprae, sobretudo nas formas lepromatosas, e este estudo reforça a importância destas células.
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Khan, Qasim. "Regulation of apoptosis in CD4§-CD8§- Ãߧ+ T cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ29310.pdf.

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Tyznik, Aaron Jacob. "CD4+ T cell help for CD8+ T cell responses /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/8314.

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Behrendt, Anne. "Differential antigen dependency of CD4+ and CD8+ T cells." Diss., Ludwig-Maximilians-Universität München, 2014. http://nbn-resolving.de/urn:nbn:de:bvb:19-171521.

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Anand, Arthi. "Characterization of CD3+CD4-CD8- (double negative) T cells in patients with systematic lupus erythematosus (SLE)." Thesis, University College London (University of London), 2003. http://discovery.ucl.ac.uk/1445261/.

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Systemic lupus erythematosus (SLE) is an autoimmune rheumatic disease characterized serologically by B cell hyperactivity and a panoply of autoantibodies against nuclear, cytoplasmic and cell surface antigens. It is thought that T cells are involved in this process and more recently it has been suggested that the CD4+ CD8+, i.e.double negative (DN) T cells, might be important. As a start to understanding the contribution of DN T cells to disease pathogenesis in SLE, the percentages of DN T cells were determined and it was found that otp but not y5 DNT cells were significantly increased in patients with SLE when compared to rheumatoid arthritis (RA) (autoimmune controls) and healthy controls. To further establish their participation in the autoimmune reactions in SLE, the activation markers expressed by the DN T cells were examined. It was found that HLA-DR and CD69, and co-stimulatory molecules CD28 and CTLA-4 were all expressed by significantly higher percentages of DNT cells from patients with SLE, than those with RA or healthy controls (HC). More DN T cells from SLE patients were CD45RA+ than from controls, while CD45R0+ were reduced. DN T cells in patients with SLE also showed a more activated phenotype than their CD4+/ CD8+ counterparts. To understand the functional significance in SLE DNT cells, the percentages of SLE otp TCR+ DN T cells containing intracellular IL-4, a Th2 cytokine was determined. Higher percentages of SLE ap TCR DN+ T cells contained DL-4 constitutively than RA or HC. DN T cell populations from patients with SLE showed greater resistance to apoptosis in culture than the conventional CD4/CD8+ cells and DN T cells from healthy controls. High Bcl-2/Bax ratios and higher levels of Bcl-x observed in the DN T cells from patients with SLE could explain their resistance to apoptosis compared to the conventional T cells and DN T cells from healthy controls.
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Freitag, Kimberly A. "Effects of Acute Nutritional Deprivation on Lymphocyte Subsets and Membrane Function in Cats." Thesis, Virginia Tech, 1998. http://hdl.handle.net/10919/46484.

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Identification of patients with suboptimal nutritional status allows for early treatment intervention. Currently, no definitive test of nutritional status exists. Therefore, this study was conducted to identify possible functional indicators of acute nutritional deprivation. The effects of total nutritional deprivation and subsequent refeeding on lymphocyte functions and subpopulations were examined in 23 healthy cats. Peripheral blood samples were analyzed at various times during fasting and refeeding periods. During the fasting period, decreases were observed in leukocyte number (day 4; p < 0.04), lymphocyte number (p < 0.02), CD4+ cells (day 4; p < 0.06), CD4:CD8 ratio (0 hours; p < 0.004), and mitogen stimulated CD4:CD8 ratio (72 hours; p < 0.15) during the fasting period as compared to baseline. Increases were seen in CD4+ cells (day 7; p < 0.09), CD8+ cells (day 7; p < 0.04) and intracellular calcium (day 4; p < 0.02) as compared to baseline. During the refeeding period increases (p < 0.05) were observed in leukocyte number, CD4+ cells, CD8+ cells, lymphocyte proliferation (p < 0.07) and lymphocyte number (p < 0.004) as compared to day 7. These findings suggest that 7 days starvation had immunosuppressive effects on cats which were alleviated during 7 days refeeding. The use of CD4:CD8 ratio in conjunction with intracellular calcium flux may be useful as indices of nutritional status.
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Books on the topic "CD8"

1

Kay, Lyndsey Sara. Anti-B-cell lymphoma activity mediated by CD3+CD4-CD8- T cells activated in vitro or in vivo. Ottawa: National Library of Canada, 2003.

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McDonagh, Mark Christian. An investigation of CD8 T lymphocyte development and function. Manchester: University of Manchester, 1994.

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Negative immunoregulatory role of CD8 T cells in peripheral tolerance. [New York]: [Columbia University], 1993.

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Ford, Megan. The role and mechanism of B6/1pr TCR[alpha beta]+CD4-CD8- T cells in immune response regulation. Ottawa: National Library of Canada, 2001.

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Carabin Is a Negative Regulator of Cd8+ T-cell-mediated Anti-tumor Immunity. [New York, N.Y.?]: [publisher not identified], 2022.

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Dumont, Caroline R. Identifying the autoantigen of a diabetogenic CD8 T cell clone isolated from Young NOD mice. [New Haven, Conn: s.n.], 1999.

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G, Janossy, Autran B, Miedema F, Commission of the European Communities., European Federation of AIDS Research., and Medical Research Council (Great Britain), eds. Immunodeficiency in HIV infection and AIDS. Basel: Karger, 1992.

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Sondermann, Bernd. Parteienfamilie ohne Zusammenhalt?: Programmatische Gegenreden von CDU, CDA und Tories auf die neue Sozialdemokratie. Frankfurt am Main: Lang, 2006.

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Cdn. 2nd ed. Warszawa: Książka i Wiedza, 1987.

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David, Rees. CDT. Harlow: Longman, 1989.

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Book chapters on the topic "CD8"

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Nelson, Robert P. "CD8 Alpha (CD8A) Deficiency." In Encyclopedia of Medical Immunology, 148–51. New York, NY: Springer New York, 2020. http://dx.doi.org/10.1007/978-1-4614-8678-7_96.

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Kleine, T. O. "Liquor-CD4/CD8-Quotient." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-49054-9_1913-1.

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Kleine, T. O. "Liquor-CD4/CD8-Quotient." In Springer Reference Medizin, 1489. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_1913.

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Nelson, Robert P. "CD8 Alpha (CD8A) Deficiency." In Encyclopedia of Medical Immunology, 1–4. New York, NY: Springer New York, 2020. http://dx.doi.org/10.1007/978-1-4614-9209-2_96-1.

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Renz, H., and B. Gierten. "CD8." In Springer Reference Medizin, 542–43. Berlin, Heidelberg: Springer Berlin Heidelberg, 2019. http://dx.doi.org/10.1007/978-3-662-48986-4_695.

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Renz, H., and B. Gierten. "CD8." In Lexikon der Medizinischen Laboratoriumsdiagnostik, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2017. http://dx.doi.org/10.1007/978-3-662-49054-9_695-1.

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Gluzman, D. F., I. V. Abramenko, N. I. Belous, L. M. Sklyarenko, L. Y. Poludnenko, S. N. Gaidukova, V. D. Drozdova, S. B. Donskaya, and E. A. Lyvshits. "CD7+CD4-CD8-Blast Cells in Acute Leukemia." In Acute Leukemias VI, 260–63. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-60377-8_43.

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Peters, Nils, Martin Dichgans, Sankar Surendran, Josep M. Argilés, Francisco J. López-Soriano, Sílvia Busquets, Klaus Dittmann, et al. "CD8 Deficiency." In Encyclopedia of Molecular Mechanisms of Disease, 295–96. Berlin, Heidelberg: Springer Berlin Heidelberg, 2009. http://dx.doi.org/10.1007/978-3-540-29676-8_302.

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Wang, Anqi, Matthias Noll, and Stefan Wesarg. "Tumorsegmentierung in CD3/CD8-gefärbten Histopathologien." In Informatik aktuell, 347–52. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-46224-9_60.

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Collins, T. L., W. C. Hahn, B. E. Bierer, and S. J. Burakoff. "CD4, CD8 and CD2 in T Cell Adhesion and Signaling." In Current Topics in Microbiology and Immunology, 223–33. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-78253-4_18.

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Conference papers on the topic "CD8"

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Наджафова, В. А. к. "Оценка нарушений субпопуляций лимфоцитов у детей с железодефицитной анемией." In General question of world science. Наука России, 2021. http://dx.doi.org/10.18411/gq-31-07-2021-03.

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Статья посвящена проблеме нарушения иммунитета у детей с железодефицитной анемией (ЖДА), проживающих в Азербайджане. Были выявлены более низкие показатели клеточного иммунитета (CD3, CD4, CD8). Относительное количество клеток CD3 в общей группе детей с ЖДА составило 52,7±4,35%, в контрольной группе - 62,6±5,49%, р<0,05. Проведенные исследования выявили положительную корреляцию клеток CD3, CD4, CD8 с гемоглобином и сывороточным железом. Результаты высокой силы связи коэффициента корреляции сывороточного железа с относительным количеством клеток CD3 и CD4 в общих группах детей с ЖДА (r = 0,8) показали, что дефицит железа играет большую роль для активности клеточного иммунитета. Таким образом, в статье показано ослабление как врожденного (NK-CD56), так и приобретенного (CD3, CD4, CD8) компонентов клеточного иммунитета. Полученные результаты могут быть оценены как нарушение иммунного баланса, связанное с недостаточностью клеточного иммунитета у детей с ЖДА.
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Lins, Lucas Costa, Iana Carneiro Pinto, Nathalia Lima Schramm dos Santos, Vitor de Oliveira Silva, and Marcos Lázaro da Silva Guerreiro. "INFLUÊNCIA DA QUIMIOTERAPIA NA RESPOSTA IMUNOLÓGICA CELULAR EM CAMUNDONGOS INFECTADOS COM AS CEPAS Y E COLOMBIANA DO TRYPANOSOMA CRUZI." In I Congresso Brasileiro de Parasitologia Humana On-line. Revista Multidisciplinar em Saúde, 2021. http://dx.doi.org/10.51161/rems/741.

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Introdução: Estudos sugerem que o tratamento quimioterápico estimula o sistema imunológico em camundongos infectados com cepas do T. cruzi. Objetivo: avaliar a influência do tratamento com Benzonidazol sobre a resposta imunológica celular em camundongos infectados com a cepa Y (suscetível) e Colombiana (resistente). Material e métodos: 150 camundongos, subdivididos em: Infectados tratados cepa Y (YT) e não tratados (Y-NT); Colombiana tratados (COL-T) e não tratados (COL-NT), Tratados não infectados (TNI) e Controles sem tratamento (CI). O inóculo foi 1,0 x 104 por via intraperitoneal. Os procedimentos estiveram de acordo ao protocolo CEUA, 013/09. O tratamento foi iniciado no pico parasitêmico das cepas, sendo no 7º dia após a infecção nos infectados pela cepa Y e, nos tratados e não infectados, no 18º nos infectados pela cepa Colombiana. A quimioterapia foi em 60 doses (100mg/kg/dia). Os camundongos eutanasiados na fase aguda e crônica nos grupos tiveram a resposta celular investigada pelas citocinas IL-6 IL-10, MCP-1, INF-γ e TNF-α e pelas subpopulações celulares no baço de CD4+ , CD8+ , células de memórias CD62L, células B e macrófagos. Resultados: as citocinas circulantes IL-6 IL-10, MCP-1, INF-γ e TNF-α, foram mais elevadas nos animais infectados com a cepa Y, o tratamento com Benz, não alterou os índices circulantes nos TNI. A citometria na fase aguda demonstrou maior frequência de CD4+ e CD8+ no grupo TNI; de células de memória (CD62L/CD4 e CD8) no grupo YT; de CD11b no grupo COL-NT fase aguda e na YT na fase crônica; e de células B (B220) no grupo CI. Na fase crônica maior frequência de CD4+ e CD8+ no grupo COL-T; de células de memória: (CD4/CD62L) no grupo YT e (CD8/CD62L) no COL-T; de CD11b no YT; e de células B (B220) CI. Conclusão: Nossos resultados sugerem que o tratamento com Benz tem influência na resposta imunológica celular em camundongos.
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"REHABILITATION MEASURES IN PATIENTS WITH OCCUPATIONAL DERMATOSES." In СОВРЕМЕННЫЕ ПРОБЛЕМЫ ЭКОЛОГИИ И ЗДОРОВЬЯ НАСЕЛЕНИЯ. ЭКОЛОГИЯ И ЗДОРОВЬЕ НАСЕЛЕНИЯ. Иркутский научный центр хирургии и травматологии, 2023. http://dx.doi.org/10.12731/978-5-98277-383-8-art12.

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The search for new modern methods of prevention and treatment of skin diseases remains relevant today. The aim is to evaluate the effectiveness of ozone therapy in the treatment of occupational allergodermatoses of chemical etiology. Materials and methods. Two groups of patients with occupational allergodermatoses (78 people) were examined and treated. Group 1 received only traditional treatment, in group 2 ozone therapy was added to the treatment complex. Immunological studies were performed using standard methods. Results. The use of ozone therapy in patients with allergodermatoses had a positive effect on cellular and humoral immunity: the content of CD3+ and CD8+-lymphocytes increased by 1,2-1,5 times, the ratio of CD4+/CD8+ normalized, the level of IL-10 and IL-4 decreased by 1,6 times, the number of patients with a positive effect according to indicators IdA, IgM, IgG, IgE accounted for more than 80 %. There was an improvement in the clinical course of the skin process, an increase in the remission period. Conclusion. The method of ozone therapy has shown its effectiveness, so it can be used for immunorehabilitation, prevention and treatment of this pathology.
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Koller, U., I. Pabinger, K. Lechner, and W. Knapp. "HEAT INACTIVATED HIGHLY PURIFIED FACTOR VIII CONCENTRATE IN THE TREATMENT OF HEMOPHILIACS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644057.

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51 severe hemophiliacs who had previously been treated with not virus-inactivated intermediate purity factor VIII concentrates were divided into two groups according to their immunological status. Group A (n=23) consisted of patients with CD4/CD8 (helper/suppressor) T cell ratio of 1.0, group B (n=28) of . patients with a ratio of 1.0. In patients of group A treatment was switched in May 1983 to highly purified heat inactivated factor VIII concentrate (BEHRINGWERKE GmbH, Marburg) whereas patients of group B continued to receive intermediate purity factor VIII concentrate. In both groups laboratory tests (clinical investigation, routine liver function tests, differential blood count, lymphocyte subpopulations and quantitative immunoglobulin analysis) were performed in May 1983 and repeated 6, 12 and 18 months thereafter. In group A a significant reduction (p 0.005) of CD8 positive cells from 10587/μl (median) to 540/μl (18 months) was observed; no significant changes of CD8 positive cells occurred in group B. CD4/CD8 ratio rose from 0.58 to 0.86 in group A (p = 0.005) and remained unchanged in group B (1.38 versus 1.23). Serum IgG decreased in both groups but was more pronounced in group A. Thus treatment with heat inactivated highly purified factor VIII improved the immunological status of hemophiliacs with an inverse ratio. Retrospective analysis of antibodies to HIV, however, showed that most of the patients of group A were antibody positive (n=21), but the 2 negative patients remained negative. In group B of the 10 HIV negative patients one became positive, all others did not change. Whether this improvement of immunological laboratory findings is of clinical relevance, remains to be established, particularly with respect to the high incidence of antibody positive patients within group A.
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Baer, Thomas M., and Louis J. Dietz. "Absolute Enumeration of Rare Cell Types in Peripheral Blood Using Laser Induced Fluorescence and Volumetric Microscopy." In Biomedical Optical Spectroscopy and Diagnostics. Washington, D.C.: Optica Publishing Group, 2006. http://dx.doi.org/10.1364/bosd.1996.ca2.

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This paper describes a novel technique for the absolute enumeration of dye-labeled cells using laser-induced fluorescence and volumetric microscopy. This technique has been used for the enumeration of CD4+ and CD8+ lymphocytes, CD34+ stem cells, and quality checking of leukocyte-reduced blood products.
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Lima, Maríllia Raphaella Cabral Fonseca de, Guilherme Antonio de Souza Silva, Leonardo Carvalho de Oliveira Cruz, Georon Ferreira de Sousa, Bárbara Rafaela da Silva Barros, Rodrigo Cesar Abreu de Aquino, and Cristiane Moutinho Lagos de Melo. "PERFIL DA RESPOSTA IMUNOLÓGICA, EFICÁCIA E EFEITOS COLATERAIS DAS VACINAS EM USO CONTRA A COVID-19 NO BRASIL." In XXVII Semana de Biomedicina Inovação e Ciência. Editora IME, 2021. http://dx.doi.org/10.51161/9786588884119/24.

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Introdução: Com a pandemia da COVID-19, doença causada pelo vírus SARS-CoV- 2¹, o desenvolvimento de uma vacina eficaz ocorreu em tempo recorde, exibindo bons resultados. As vacinas foram criadas com métodos variados, como: vetor viral recombinante, subunidade de proteína, vírus inativados e ácidos nucleicos. No Brasil, a vacinação foi iniciada em 17 de janeiro de 2021, com uso emergencial da CoronaVac e da AstraZeneca. Objetivos: Descrever o perfil da resposta imunológica, eficácia e efeitos colaterais das vacinas contra COVID-19 utilizadas no Brasil. Métodos: Foram selecionados artigos disponíveis na plataforma PubMed. Os critérios de inclusão foram artigos no idioma inglês, publicados nos anos de 2020-2021 com os seguintes descritores: Vacinação; COVID-19; Eficácia; Efeitos Colaterais; e Resposta Imunológica. Resultados/Discussão: A vacina de RNAm da Pfizer induziu elevados títulos de anticorpos neutralizantes para SARS-CoV-2 e resposta T CD4+/CD8+, com eficácia de 95% após a segunda dose, e os efeitos colaterais mais comuns foram fadiga e cefaléia². A AstraZeneca, vacina de adenovírus modificado, apresentou resposta imunológica humoral e celular, além de eficácia de 90% após a segunda dose, possuindo como efeitos adversos mais comuns mialgia, fadiga, cefaléia, dor no local da injeção e febre.³ A Janssen, vacina adenoviral (Ad26) de dose única, demonstrou produção de anticorpos neutralizantes, e resposta celular T CD4+/CD8+, apresentando eficácia variando de 67% a 85% de acordo com a gravidade do caso. Seus os principais efeitos adversos foram dor no local da injeção, cefaléia e fadiga.4 A Sputnik V, vacina adenoviral de imunização heteróloga (Ad26 primeira dose e Ad5 segunda dose), apresentou forte resposta humoral e de células T CD4+/CD8+, e eficácia de 91,4% após a segunda dose, sendo as principais reações adversas astenia, mialgia, artralgia, febre e cefaléia. A CoronaVac, vacina de vírus inteiro inativado, induziu a produção de anticorpos contra o SARS-CoV-2, além de uma resposta celular de linfócitos T produtores de IFN-γ, apresentou eficácia que variou entre 83,7% e 100% após a segunda dose, dependendo da gravidade, e a principal reação adversa foi a dor no local da aplicação.5 Conclusões: O perfil de resposta imunológica é, principalmente, produção de anticorpos neutralizantes e resposta T CD4+/CD8+. As eficácias das vacinas em uso no Brasil variam entre 66% - 91,4% após a imunização completa e o principal efeito colateral foi a cefaléia.
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Jiang, W., H. Hong, R. Juskevicius, D. A. Weidner, Y. Feng, L. V. Yang, J. Q. Lu, and X. H. Hu. "Study of 3D Structural Differences between CD4+ and CD8+ T lymphocytes." In Biomedical Optics. Washington, D.C.: OSA, 2014. http://dx.doi.org/10.1364/biomed.2014.bs3a.78.

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Zafar, Muhammad Mohsin, Zunaira Rauf, Anabia Sohail, and Asifullah Khan. "Lymphocyte Annotator: CD3+ and CD8+ IHC Stained Patch Image Annotation Tool." In 2020 International Symposium on Recent Advances in Electrical Engineering & Computer Sciences (RAEE & CS). IEEE, 2020. http://dx.doi.org/10.1109/raeecs50817.2020.9265757.

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Ting, Vox, Carmen Chak-Lui Wong, Yok Lam Kwong, and Thomas Chung Cheung Yau. "Abstract 487: Genomic difference of CD4 CD8 double-positive T cells versus conventional CD4 T cells and CD8 T cells in responders undergoing immunotherapy in advanced HCC." In Proceedings: AACR Annual Meeting 2021; April 10-15, 2021 and May 17-21, 2021; Philadelphia, PA. American Association for Cancer Research, 2021. http://dx.doi.org/10.1158/1538-7445.am2021-487.

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Jackute, Jurgita, Marius Zemaitis, Darius Pranys, Brigita Sitkauskiene, Skaidrius Miliauskas, and Raimundas Sakalauskas. "Distribution of CD4+Foxp3+,CD4+ and CD8+ T cells in non-small cell lung cancer." In Annual Congress 2015. European Respiratory Society, 2015. http://dx.doi.org/10.1183/13993003.congress-2015.pa531.

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Reports on the topic "CD8"

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Knutson, Keith L. CD8 T Cells and Immunoediting of Breast Cancer. Fort Belvoir, VA: Defense Technical Information Center, August 2008. http://dx.doi.org/10.21236/ada624685.

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Banai, Menachem, and Gary Splitter. Molecular Characterization and Function of Brucella Immunodominant Proteins. United States Department of Agriculture, July 1993. http://dx.doi.org/10.32747/1993.7568100.bard.

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The BARD project was a continuation of a previous BARD funded research project. It was aimed at characterization of the 12kDa immunodominant protein and subsequently the cloning and expression of the gene in E. coli. Additional immunodominant proteins were sought among genomic B. abortus expression library clones using T-lymphocyte proliferation assay as a screening method. The 12kDa protein was identified as the L7/L12 ribosomal protein demonstrating in the first time the role a structural protein may play in the development of the host's immunity against the organism. The gene was cloned from B. abortus (USA) and B. melitensis (Israel) showing identity of the oligonucleotide sequence between the two species. Further subcloning allowed expression of the protein in E. coli. While the native protein was shown to have DTH antigenicity its recombinant analog lacked this activity. In contrast the two proteins elicited lymphocyte proliferation in experimental murine brucellosis. CD4+ cells of the Th1 subset predominantly responded to this protein demonstrating the development of protective immunity (g-IFN, and IL-2) in the host. Similar results were obtained with bovine Brucella primed lymphocytes. UvrA, GroE1 and GroEs were additional Brucella immunodominant proteins that demonstrated MHC class II antigenicity. The role cytotoxic cells are playing in the clearance of brucella cells was shown using knock out mice defective either in their CD4+ or CD8+ cells. CD4+ defective mice were able to clear brucella as fast as did normal mice. In contrast mice which were defective in their CD8+ cells could not clear the organisms effectively proving the importance of this subtype cell line in development of protective immunity. The understanding of the host's immune response and the expansion of the panel of Brucella immunodominant proteins opened new avenues in vaccine design. It is now feasible to selectively use immunodominant proteins either as subunit vaccine to fortify immunity of older animals or as diagnostic reagents for the serological survaillance.
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Bullock, Timothy N., and Kimberly A. Kelly. Functional Proteomics to Identify Moderators of CD8+ T-Cell Function in Melanoma. Fort Belvoir, VA: Defense Technical Information Center, September 2013. http://dx.doi.org/10.21236/ada599199.

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Bullock, Timothy N., and Kimberly A. Kelly. Functional Proteomics to Identify Moderators of CD8+ T-Cell Function in Melanoma. Fort Belvoir, VA: Defense Technical Information Center, October 2014. http://dx.doi.org/10.21236/ada618873.

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Bullock, Timothy N., and Kimberly A. Kelly. Functional Proteomics to Identify Moderators of CD8+ T Cell Function in Melanoma. Fort Belvoir, VA: Defense Technical Information Center, May 2015. http://dx.doi.org/10.21236/ada625202.

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Mekova, Ralitsa V., Spaska S. Lesichkova, Adelina D. Tsakova, Julieta Z. Bakalova, Deniz Bakalov, and Mihail Boyanov. Circulating CD3(+)CD4(+)CD28(‒) T Lymphocytes in Patients with Autoimmune Thyroiditis. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, May 2020. http://dx.doi.org/10.7546/crabs.2020.05.14.

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Lee, Chung, Timothy Kuzel, Richard Meagher, Ximing Yang, Norm Smith, and Qiang Zhang. Preparation for a Clinical Trial Using Adoptive Transfer of Tumor-Reactive TGF_Beta-Insensitive CD8+ T Cells for Treatment of Prostate Cancer. Fort Belvoir, VA: Defense Technical Information Center, July 2006. http://dx.doi.org/10.21236/ada462885.

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Lee, Chung. Preparation for a Clinical Trial Using Adoptive Transfer of Tumor-Reactive TGF_Beta-Insensitive CD8+ T Cells for Treatment of Prostate Cancer. Fort Belvoir, VA: Defense Technical Information Center, July 2006. http://dx.doi.org/10.21236/ada463479.

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Dobrzanski, Mark J. Analysis of Tumor Antigen-Specific Tc1 and Tc2 CD8 Effector Cell Subpopulations as Potential Therapeutics Agents in the Treatment of Progressive Breast. Fort Belvoir, VA: Defense Technical Information Center, September 2004. http://dx.doi.org/10.21236/ada429842.

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Leitner, Gabriel, and Naomi Balaban. Novel Immunotherapeutic Agent for the Treatment and Prevention of Staphylococcal Mastitis in Dairy Cows. United States Department of Agriculture, January 2009. http://dx.doi.org/10.32747/2009.7709880.bard.

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Staphylococci are the most common and costly mammary disease of dairy cattle worldwide. TRAP, a membrane associated 167AA protein, is highly conserved among staphylococci. The aims of this study were to test the safety and efficacy of recombinant TRAP (rTRAP) vaccine in dairy animals. The vaccine was safe as 2-3 subcutaneous injections of rTRAP (54–100μg) with adjuvant ISA 206 to cows and goats did not lead to any abnormal symptoms of sensitivity to the vaccine. The rTRAP vaccine was immunogenic and caused the induction of a humoral immune response that remained high for at least 160 days post second immunization. rTRAP vaccine also elicited a cell-mediated immune response (memory CD4+ and CD8+ T cells), as determined by lymphocyte proliferation assays. The rTRAP vaccine was efficacious as at parturition, only 13.5% heifers in the immunized group were infected with Staphylococcus chromogenes as compared to 42.9% in the non immunized group. Additionally, when cows were immunized in mid-lactation, the difference between somatic cell count (SCC) in immunized and control animals was profound (45±7 vs. 470±194, respectively). At the same time, the difference in milk yield was also evident (48.3±1.4 vs. 44.3±0.9 l/day, respectively). Put together, these studies indicate the value of the rTRAP vaccine in preventing new udder infections by staphylococci, which significantly lead to lowered SCC and some increase in milk yield. TRAP is conserved among all strains and species and is constitutively expressed in any strain of S. aureus or CNS tested so far, including those isolated from cows. TRAP may thus serve as a universal anti-staphylococcus vaccine.
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