Journal articles on the topic 'Cattle Spermatozoa Storage'
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Rizal, Muhammad, Muhammad Riyadhi, Bambang Irawan, Anis Wahdi, Habibah Habibah, and Herdis Herdis. "Daya Hidup Spermatozoa Epididimis Sapi Persilangan yang Dipreservasi dengan Air Kelapa Muda pada Suhu 5oC (VIABILITY OF EPIDIDYMAL SPERMATOZOA CROSSBREED CATTLE PRESERVED WITH COCONUT WATER AT 5oC)." Jurnal Veteriner 18, no. 4 (January 23, 2018): 571. http://dx.doi.org/10.19087/jveteriner.2017.18.4.571.
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The objective of this research was to examine the effectiveness of coconut water with various egg yolk concentrations in maintaining the viability of epididymal spermatozoa of crossbreed cattle preserved at 5oC. Five testis with epididymides of crossbreed cattle were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure methods of cauda epididymides with saline solution (0.9% NaCl). Collected-spermatozoa was equally divided in volume into four tubes and diluted with lactose extender containing 20% egg yolk (control), 90% coconut water + 10% egg yolk (CWEY10), 85% coconut water + 15% egg yolk (CWEY15), and 80% coconut water + 20% egg yolk (CWEY20), repectively. Diluted-spermatozoa was stored in refrigerator at 5oC. Quality of dilutedspermatozoa including percentages of motile spermatozoa (MS), live spermatozoa (LS), spermatozoa with intact plasma membrane (IPM) were evaluated every day during four days of storage. Data were analyzed by using completely randomized design with four treatments and five replicates. Means were compared with significant difference test at 0.05 significant level. Results of this study showed that mean of spermatozoa concentration, percentage of MS, percentage of LS, percentage of spermatozoa abnormal, and percentage of IPM of crossbreed cattle fresh epididymal spermatozoa were 1,414 million cell/ml, 72%, 85%, 9%, and 90%, respectively. At day-4 of the storage, percentages of MS, LS, and IPM of control (43, 52.2, 59.2%) and CWEY20 (42, 52, 59%) were significantly (P<0.05) higher than CWEY10 (33, 45.4, 52.8%) and CWEY15 (37, 50, 54.6%). In conclusion, lactose and CWEY20 extenders could be maintaining the quality of epidydimal spermatozoa of crossbreed cattle for three days preservation at 5oC and two days for CWEY10 and CWEY15.
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Suarez, S. S. "Interactions of spermatozoa with the female reproductive tract: inspiration for assisted reproduction." Reproduction, Fertility and Development 19, no. 1 (2007): 103. http://dx.doi.org/10.1071/rd06101.
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Artificial insemination with sexed semen, in vitro fertilisation and intracytoplasmic sperm injection have been used to reproduce animals, but often not as successfully as natural mating. Learning more about how spermatozoa normally interact with the female tract can provide inspiration for developing improvements in assisted reproduction. The present review focuses on Bos taurus, because more is known about this species than others. At coitus, bull spermatozoa are deposited into the anterior vagina, where they rapidly enter the cervix. Cervical mucus quickly filters out seminal plasma from spermatozoa, unlike most assisted reproduction protocols. Spermatozoa that reach the uterus may require certain cell surface proteins to swim through the uterotubal junction. Shortly after passing through the junction, most spermatozoa are trapped in a storage reservoir by binding to oviducal epithelium, in the case of cattle via bovine seminal plasma (BSP) proteins coating the sperm head. As ovulation approaches, spermatozoa capacitate and shed BSP proteins. This reduces sperm binding to the epithelium and releases them from storage. Motility hyperactivation assists spermatozoa in leaving the storage reservoir, swimming through oviducal mucus and the cumulus oophorus, and penetrating the oocyte zona pellucida. Chemotactically regulated switching between asymmetrical (i.e. hyperactivated) and symmetrical flagellar beating may also guide spermatozoa to the oocyte.
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Susandani, Oky, Tri Wahyu Suprayogi, Ratna Damayanti, and Anwar Ma'ruf. "Factors Affecting Fresh Semen Quality in Pasundan Cattle at UPTD BPPIBTSP Ciamis." Journal of Applied Veterinary Science And Technology 2, no. 2 (October 30, 2021): 37. http://dx.doi.org/10.20473/javest.v2.i2.2021.37-42.
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Background: Pasundan cattle are local cattle native to Indonesia. One way to conserve beef cattle genetics is to use Artificial Insemination technology. The success of Artificial Insemination can be influenced by the quality of semen. Purpose: To determine factors affecting fresh semen quality in Pasundan cattle at UPTD BPPIBTSP Ciamis. Methods: The data were obtained through observations on seven Pasundan bulls in March 2021 towards fresh semen quality and some factors influencing it. The Pasundan bulls observed were seven productive males. Results: The fresh semen quality of Pasundan cattle, such as volume, color, and pH, showed good result,s but the average consistency and concentration of spermatozoa were still below the standard. The factors that can affect the fresh semen quality are the breed of beef cattle, age, body weight, feed, season, exercise, and frequency of semen storage. Conclusion: The determining factors that can cause the consistency and concentration of Pasundan cattle’s spermatozoa at UPTD BPPIBTSP Ciamis are feed and season.
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Coester, Jacky Setiofan, Abrani Sulaiman, and Muhammad Rizal. "Daya Hidup Spermatozoa Sapi Limousin yang Dipreservasi dengan Pengencer Tris dan Berbagai Konsentrasi Sari Kedelai." Jurnal Ilmu dan Teknologi Peternakan Tropis 6, no. 2 (August 6, 2019): 175. http://dx.doi.org/10.33772/jitro.v6i2.6023.
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ABSTRAK digunakan sebagai salah satu bahan pengencer semen pengganti kuning telur ayam. Penelitian ini bertujuan untuk menguji pengaruh sari kedelai dalam pengencer Tris terhadap motilitas dan daya hidup spermatozoa sapi limousin yang dipreservasi pada suhu 5oC. Semen ditampung dengan vagina buatan. Semen segar yang memenuhisyarat dibagi kedalam empat buah tabung reaksi yang masing-masing berisi pengencer perlakuan, yakni: 80% pengencer dasar Tris + 20% kuning telur (Tris), 97% pengencer dasar Tris + 3% sari kedelai (SK3), 95% pengencer dasar Tris + 5% sari kedelai (SK5), dan 93% pengencer dasar Tris + 7% sari kedelai (SK7). Semen yang telah diencerkan disimpan di dalam refrigerator pada suhu 5oC, dan dievaluasi motilitas dan viabilitas spermatozoa setiap hari hingga hari kelima. Hasil penelitian menunjukkan bahwa motilitas dan viabilitas spermatozoa di dalam pengencer Tris nyata (P<0,05) lebih tinggi dibandingkan dengan spermatozoa di dalam pengencer SK3, SK5, dan SK7 selama empat hari penyimpanan. Berdasarkaan hasil penelitian dapat disimpulkan bahwa pengencer Tris-kuning telur lebih baik dalam mempertahankan motilitas dan daya hidup spermatozoa sapi limousin dibandingkan dengan pengencer Tris-sari kedelai yang dipreservasi pada suhu 5°C. Pengencer Tris dengan konsentrasi 3% sari kedelai lebih baik dibandingkan dengan konsentrasi 5% dan 7%.Kata Kunci : sapi limousine, sari kedelai, semen, trisABSTRACTSoybean contains anlecithin (phosphatidyl choline), that has the potential to be used as substitute for chicken egg yolk as one of the semen extender compound. The aim of this research was to examine the effect of soybean juice in Tris extender on the motility and viability of Limousin cattle spermatozoa preserved at 5oC. Semen was collected using artificial vagina. Fresh semen was divided into four tubes containing a treatment extender, i.e. 80% Tris base extender + 20 egg yolk (Tris), 97% Tris-based extender + 3% soybean juice (SJ3), 95% Tris-based extender + 5% soybean juice (SJ5), 93% Tris-based extender + 7% soybean juice (SJ7), respectively. Diluted-semen was preserved in refrigerator at 5oC, and evaluation of spermatozoa motility and viability were conducted on daily basis up to five days. The result showed that percentages of motility and viability of spermatozoa in Tris-yolk extender were significantly (P<0.05) higher than spermatozoa in SJ3, SJ5, and SJ7extenders during four days of storage. In conclusion, Tris-yolk extender is better than Tris-soybean juice in maintaining the spermatozoa motility and viability of Limousin cattle preserved at 5°C. Tris extender containing 3% soybean juice is better than 5% and 7%.Keywords: limousin cattle, semen, soybean juice, tris
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Azura, Sarah, Hermin Ratnani, Suherni Susilowati, Mas'ud Hariadi, Abdul Samik, and Koesnoto Soepranianondo. "Effect of α-tocopherol supplementation in diluents on the motility, viability and plasma membrane integrity of Simmental bull spermatozoa after cooling." Ovozoa : Journal of Animal Reproduction 9, no. 1 (May 11, 2020): 1. http://dx.doi.org/10.20473/ovz.v9i1.2020.1-6.
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Semen storage in cold temperatures might cause an increase in reactive oxygen species (ROS) production. This condition resulted in spermatozoa damage and quality decrease. This study was conducted to investigate the effects of α-tocopherol supplementation in diluents on the motility, viability, and plasma membrane integrity of Simmental bull spermatozoa after cooling. Semen samples were diluted in skim milk egg yolk supplemented with 0, 0.5, 1.0, and 1.5 mM α-tocopherol respectively for control, Tl, T2, and T3. Spermatozoa were evaluated for their motility, viability, and membrane integrity in cooling temperature (5°C). The daily evaluation showed that 1.5 mM α-tocopherol was the best in maintaining motility, viability, and plasma membrane integrity, while 1.0 mM α-tocopherol was only good for maintaining viability. Therefore, it can be concluded that α-tocopherol at the concentration of 1.5 mM was an efficient antioxidant supplement for Simmental cattle semen in skim milk egg yolk diluent.
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Marawali, ALoysius, Muhammad S. Abdullah, and Jalaludin Jalaludin. "Efektivitas Suplementasi Filtrat Jambu Biji dalam Pengencer AirKelapa-Kuning Telur terhadap Kualitas Semen Cair Sapi Bali (THE EFFECTIVENESS OF GUAVA FILTRATE SUPPLEMENTATION IN COCONUT WATER-EGG YOLK DILUTION ON QUALITY OF LIQUID SEMEN OF BALI CATTLE)." Jurnal Veteriner 20, no. 1 (May 24, 2019): 20. http://dx.doi.org/10.19087/jveteriner.2019.20.1.20.
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The aim of this research was to know the effectiveness of guava filtrate supplementation in coconut water- egg yolk dilution on quality of liquid semen stored at 5oC of Bali cattle. Semen collected from a five year old Bali cattle using artificial vagina. Semen of good quality were kept in six tubes based on treatment then stored at 5oC. Treatments of the research were P0 : coconut water 80% + egg yolk 20% without guava filtrate; P1 : coconut water 80% + egg yolk 20% + 0.8% guava filtrate; P2 : coconut water 80% + egg yolk 20% + 0.9% guava filtrate; P3 : coconut water 80% + egg yolk 20% + 1.0 % guava filtrate; P4 : coconut water 80% + egg yolk 20% + 1.1 % guava filtrate and P5 : coconut water 80% + egg yolk 20% + 1.2 % guava filtrate. Each treatment was replicated 8 times making 48 experimental units. Results of the study showed that percentage mean of motility, viability, MPU, and TAU of spermatozoa after three days storage for P0 were : 42.20%, 41.85%, 39.08% and 40.90%; P1 : 50.40%, 53.89%, 52.99% and 54.67%; P2 : 54.67%, 56.97%, 54.51% and 54.36%; P3 : 17.00%, 29.96%, 29.64% and 29.64%; P4 : 23.38%, 24.64%, 21.06% and 24.45%Jurnal Veteriner Maret 2019 Vol. 20 No. 1 : 20 -29 pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2019.20.1.20 Terakreditasi Nasional, Dirjen Penguatan Riset dan Pengembangan, online pada http://ojs.unud.ac.id/index.php/jvet Kemenristek Dikti RI S.K. No. 36a/E/KPT/201621PENDAHULUAN Salah satu solusi yang dapat digunakan untuk pengembangan program Inseminasi buatan (IB) secara cepat dan mudah pada sapi bali adalah penggunaan semen cair. Penggunaan semen cair dapat meningkatkan kinerja IB pada sapi bali di Nusa Tenggara Timur (NTT). Keunggulan lain semen cair dapat diproduksi menggunakan bahan pengencer herbal berbasis bahan lokal dan peralatan yang sederhana serta mudah diperoleh dan tidak tergantung dengan persediaan nitrogen cair. Hasil akhir dari metabolisme spermatozoa adalah terbentuknya radikal bebas berupa derivat oksigen di antaranya adalah single1 oksigen (1O2), tripel1 oksigen (3O2), superokside anion (O2-), hidroksil radikal (OH) dan nitrit oxide (NO-) yang semuanya disebut radical oksigen species (ROS). Single1 oksigen dapat merusak ikatan rangkap pada asam lemak sehingga dapat menyebabkan kerusakan Deoxyribo Nuclead Acid (DNA) dan protein. Single1 oksigen bila bereaksi dengan asam amino histidin akan membentuk enzim yang dapat menyebabkan denaturasi protein. Kerusakan spermatozoa pada penyimpanan suhu 5%C akibat radikal bebas dan cold shock inilah merupakan penyebab utama disfungsi semen (Sharma et al., 2000). Oksidasi fosforilasi yang terganggu menyebabkan peningkatan radikal bebas dalam semen. Kadar radikal bebas yang terganggu menyebabkan peningkatan radikal bebas dalam semen. Kadar radikal bebas yang tinggi dalam sel dapat mengoksidasi lipid, protein dan DNA. Lipid membran plasma semen memiliki fosfolipid dengan kadar yang tinggi menyebabkan semen rentan terhadap radikal bebas (Sanoeka dan Kurpisz, 2004). Antioksidan bertindak mengikat asam lemak tak jenuh dan mencegah terjadinyareaksi berantai. Pada proses penyimpanan semen akan terjadi kerusakan membran plasma spermatozoa akibat terbentuknya perioksidasi lipid. Antioksidan-pemutus rantai seperti yang terkandung dalam jambu biji dapat menghambat perioksidasi lipid dalam membran melalui radical peroxyl (RO) dan alkoxyl (ROO) pengurai. Pengunaan jambu biji yang difilter dalam pengencer air kelapa kuning telur dapat menjaga kualitas spermatozoa (motilitas, keutuhan akrosom, viabilitas dan morfologi spermatozoa) semen cair sapi bali selama penyimpanan pada suhu 5%C. Dosis jambu biji yang difilter yang terbaik dalam pengencer air kelapa kuning telur, akan terbaik pula dalam mempertahankan kualitas spermatozoa sampai tujuan IB. Adapun tujuan penelitian ini adalah menguji berbagai level pemberian filtrat jambu biji (FJB) dalam pengencer air kelapa kuning telur terhadap motilitas, viabilitas, membran plasma utuh (MPU) dan tudung akrosom utuh (TAU) spermatozoa sapi bali yang disimpan pada suhu 5%C.METODE PENELITIAN Penelitian ini telah dilakukan di Laboratorium Reproduksi milik Yayasan Wiliams dan Laura yang berlokasi di Tilong, Desa Oelnasi, Kec. Kupang Tengah, Kab. Kupang, Nusa Tenggara Timur, dan berlangsung selama delapan bulan. Materi yang digunakan dalam penelitian ini adalah semen sapi bali yang ditampung dari satu ekor sapi bali jantan berumur lima tahun milik Yayasan Williams dan Laura yang telah dilatih, memiliki performans yang baik, dan organ reproduksi normal. Pakan yang diberikan adalah hijauan berupa rumput dan legum dan pemberian konsentrat secukupnya (dedak padi dan jagung giling).and P5 : 9%, 21.25%, 17.56% and 19.30%. Result of statistical analysis showed that there were a significant effect (P<0.05) between treatment on motility, viability, MPU and TAU of spermatozoa of Bali cattle till the third day of storage. It can be concluded that the supplementation of guava filtrate 0.9% in dilution of coconut water 80% - egg yolk 20% had been able to maintain motility, viability, MPU and TAU of spermatozoa of Bali cattle till the third day of storage at 5oC.
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Talevi, R., V. Barbato, S. De Iorio, V. Mollo, T. Capriglione, L. Ricchiari, A. Samo, and R. Gualtieri. "Is there a role for endocannabinoids in sperm–oviduct interaction?" REPRODUCTION 140, no. 2 (August 2010): 247–57. http://dx.doi.org/10.1530/rep-10-0095.
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The endocannabinoid system (ECS) has been found in reproductive cells and tissues in several mammals. Spermatozoa are able to respond to anandamide, and the oviduct is able to synthesize and modulate the concentration of this endocannabinoid along the isthmic and ampullary regions. The main aim of this study was to understand whether the ECS has a role during sperm storage and release within the oviduct in cattle. Data showed that 1) the endocannabinoid receptors 1 and 2 (CB1 and CB2) are present in bovine spermatozoa both in the initial ejaculate and in spermatozoa bound to the oviduct in vitro; 2) CB1 receptor is still detectable in spermatozoa released from the oviduct through penicillamine but not in those released through heparin; 3) arachidonylethanolamide (AEA) does not affect sperm viability, whereas it depresses sperm progressive motility and kinetic values; 4) sperm–oviduct binding and release in vitro are not influenced by AEA; 5) AEA depresses sperm–zona pellucida (ZP) binding; 6) binding of heparin-capacitated spermatozoa to the ZP is not affected by AEA; 7) N-acylphosphatidylethanolamine-selective phospholipase D, the main enzyme involved in anandamide synthesis, is expressed in oviductal epithelial cells. In conclusion, secretion of AEA from epithelial cells might contribute to the oviduct sperm-reservoir function, prolonging the sperm fertile life through the depression of motility and capacitation. Capacitation signals, such as heparin, that promote sperm release, might remodel the sperm surface and cause a loss of the sperm sensitivity to AEA.
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Khaled Taïbi, Mohamed Achir,, Leila Ait Abderrahim, Mohamed Boussaid, Kada Souana, Abdelkader Tadj, Toufik Benaissa, and Tayeb Gouchich. "Dissecting the relationship between artificial insemination success and bull semen quality in the arid region of Tiaret (Algeria)." Bionatura 7, no. 1 (February 15, 2022): 1–5. http://dx.doi.org/10.21931/rb/2022.07.01.18.
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Despite being subject to prior assortment, frozen bull sperms commercialized for artificial insemination may present certain morphological defects. The present study aims (i) to assess the artificial insemination success of the most common cattle breeds in Algeria and (ii) to evaluate the possible effects of commercialized bull’s semen quality on this operation. Artificial insemination was assessed through four years of field monitoring by inseminating different cattle breeds of normal fertility. However, semen quality was evaluated using light microscopy by measuring viability, motility, and morphological abnormalities of spermatozoa. Field study revealed a high percentage of normal calving in red and white Holstein breed (44.83 %) against the high percentage of embryonic mortality (46.43 %) and calving with a malformation (10.71 %) in Montbéliarde breed. Semen quality assessment revealed that sperm viability and motility were higher in Holstein breeds than in Montbéliarde. Furthermore, significant differences between semen bulls were found in the proportion of abnormal spermatozoa; a higher rate of sperms with the abnormal head was observed in the black and white Holstein breed (69.3±10.98 %). However, the percentage of abnormal sperms with tail defects was higher in the Montbéliarde breed (67.5±10.74 %). The lousy quality of the selected semen and/or the poor handling and storage of frozen semen constitute a determinant factor that hinders the success of artificial insemination in the arid region of Tiaret (Algeria).
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Yanagimachi, Ryuzo, Haruo Katayose, Gary Killian, Chin N. Lee, Douglas T. Carrell, and Thomas T. F. Huang. "Moderate heat treatment increases the penetrability of zonae pellucidae of salt-stored mammalian oocytes by spermatozoa." Zygote 1, no. 4 (November 1993): 345–51. http://dx.doi.org/10.1017/s0967199400001672.
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SummaryThe zona pellucida of mammalian oocytes stored in highly concentrated solutions of neutral salts is known to retain its biological and biochemical properties. However, the zona may become resistant to sperm penetration as the storage period is increased. In cattle and hamsters, the penetrability of zonae of salt-stored oocytes was restored or increased by treating the oocytes with moderate heat without altering the gross morphology of the zona. Although this technique did not work for salt-stored human ova, this may have been due to the use of so-called inseminated- unfertilised ova which (1) may have been fertilised but failed to activate, or (2) were not fertilised because of functionally defective zonae.
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Pero, M. E., G. J. Killian, P. Lombardi, L. Zicarelli, L. Avallone, and B. Gasparrini. "327 IDENTIFICATION OF OSTEOPONTIN IN WATER BUFFALO SEMEN." Reproduction, Fertility and Development 19, no. 1 (2007): 279. http://dx.doi.org/10.1071/rdv19n1ab327.
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The competitiveness of buffalo breeding will depend on the utilization of reproductive biotechnologies that allows acceleration of genetic progress. A major factor hampering the efficiency of both artificial insemination and in vitro embryo production programs in this species is male hypofertility. Reports for several species suggest that seminal plasma contains factors that influence male fertility. Osteopontin is a glycoprotein found in several biological fluids including seminal plasma, and its presence is associated with spermatozoa concentration. In cattle, expression of osteopontin was highly correlated with bull fertility, and it was proposed to be a marker to predict male fertility (Cancel et al. 1999 Biol. Reprod. 60, 454–460). No data are available about the presence or activity of osteopontin in water buffalo. The aim of this preliminary study was to determine if osteopontin is present in buffalo semen and to evaluate whether freezing procedures cause the loss of osteopontin from spermatozoa. Semen was collected in authorized semen collection centers from 6 buffalo bulls by using an artificial vagina. A collection of bovine semen was used as a positive control. An aliquot from each sample was frozen using standard procedures for semen storage. Each ejaculate was centrifuged at 600g for 10 min at room temperature, and the supernatant was recovered and centrifuged at 10 000g for 1 h at 4�C. The total protein concentration in seminal plasma and spermatozoa was determined by the Bradford method, using ovoalbumin as the standard. Proteins (50 �g) were separated by electrophoresis and analyzed by western blotting (Cancel et al. 1999). Polyclonal antibodies against bovine milk osteopontin were prepared as previously described (Cancel et al. 1997 Biol. Reprod. 57, 1293–1301). The intensities of bands indicated by western blot were quantified by densitometer. Osteopontin was detected in all samples of buffalo semen. Most of the osteopontin detected was in the seminal plasma. Relative amounts of osteopontin detected in spermatozoa were 50% or less of that detected in seminal plasma; furthermore, the protein was not found in sperm from all bulls. These results suggest that most osteopontin is produced by the ampullae and seminal vesicles, similar to what was reported for cattle (Cancel et al. 1999). Semen frozen by standard procedures showed a reduction in amount of osteopontin by up to 50%. These studies suggest that the fertility-associated protein osteopontin may be useful as a sensitive tool to evaluate whether sperm storage procedures are detrimental and result in excess loss of osteopontin from sperm. In conclusion, the results have demonstrated that osteopontin is present in buffalo seminal plasma and sperm. Further studies will examine whether the expression of osteopontin is correlated with the fertility of buffalo bulls, as has been demonstrated in bovine bulls.
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Sabile, Sahiruddin, Abdul Latief Toleng, Muhammad Yusuf, Zulkharnaim, Sri Firmiaty, Muhammad Idrus, and Nasriyanto. "PENGARUH PENAMBAHAN EKSTRAK BUAH MENGKUDU (Morinda citrifolia Linn) DALAM PENGENCER TERHADAP MOTILITAS SPERMATOZOA PADA SEMEN CAIR SAPI BALI." AVES: Jurnal Ilmu Peternakan 10, no. 2 (December 8, 2016): 2. http://dx.doi.org/10.35457/aves.v10i2.186.
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The processing of liquid cement is likely to decrease sperm motility. It can be caused by the oxidative reaction to increase the amount of free radicals. Noni (Morinda citrifolia Linn) contains many antioxidants that can serve counteract free radicals. This study aims to determine the addition of extracts of noni (Morinda citrifolia Linn) in order to maintain the individual motility of spermatozoa in the semen liquid Bali cattle. This research was conducted at the Laboratory of Animal Reproduction Semen Processing Unit Faculty of Animal Husbandry Hasanuddin University Makassar. Extracts of noni fruit (Morinda citrifolia Linn) effect on sperm motility (P <0.05). Storage P0 at 3 h to day 2 showed higher motility. Concentration 0,02 gr/ml (P1) gives the best results in maintaining sperm motility on day 3rd to 5th. Extracts of noni fruit (Morinda citrifolia Linn) can retain sperm motility because many contain many antioxidants that serve to neutralize free radicals cause a decrease in sperm motility during equilibration at 5 0C.
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Sabile, Sahiruddin, Abdul Latief Toleng, Muhammad Yusuf, Zulkharnaim, Sri Firmiaty, Muhammad Idrus, and Nasriyanto. "PENGARUH PENAMBAHAN EKSTRAK BUAH MENGKUDU (Morinda citrifolia Linn) DALAM PENGENCER TERHADAP MOTILITAS SPERMATOZOA PADA SEMEN CAIR SAPI BALI." AVES: Jurnal Ilmu Peternakan 10, no. 2 (December 8, 2016): 2. http://dx.doi.org/10.30957/aves.v10i2.186.
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The processing of liquid cement is likely to decrease sperm motility. It can be caused by the oxidative reaction to increase the amount of free radicals. Noni (Morinda citrifolia Linn) contains many antioxidants that can serve counteract free radicals. This study aims to determine the addition of extracts of noni (Morinda citrifolia Linn) in order to maintain the individual motility of spermatozoa in the semen liquid Bali cattle. This research was conducted at the Laboratory of Animal Reproduction Semen Processing Unit Faculty of Animal Husbandry Hasanuddin University Makassar. Extracts of noni fruit (Morinda citrifolia Linn) effect on sperm motility (P <0.05). Storage P0 at 3 h to day 2 showed higher motility. Concentration 0,02 gr/ml (P1) gives the best results in maintaining sperm motility on day 3rd to 5th. Extracts of noni fruit (Morinda citrifolia Linn) can retain sperm motility because many contain many antioxidants that serve to neutralize free radicals cause a decrease in sperm motility during equilibration at 5 0C.
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Arat, S., S. Pabuccuoglu, H. Sagirkaya, K. Demir, R. Arici, B. Ustuner, S. Alcay, et al. "22 SEMEN AND REPRODUCTIVE PROFILES OF CLONED ANATOLIAN GREY CATTLE." Reproduction, Fertility and Development 27, no. 1 (2015): 103. http://dx.doi.org/10.1071/rdv27n1ab22.
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Anatolian grey cattle (endangered native Anatolian cattle) as 1 male (clone 1) and 4 females (clones 2–5) were produced from cells of 1 male and 1 female cattle by somatic cell nuclear transfer (SCNT) in a previous study. In this study, we examined the reproductive potential of these cloned animals, which are now 4 and 5 years old. The parameters evaluated by phase contrast microscopy for motility, TUNEL for DNA fragmentation, eosin staining for viability, Hoechst 33258 staining and hypo-osmotic swelling test (HOST) for membrane integrity, and fluorescein isothiocyanate-Pisum sativum agglutinin (FITC-PSA) for acrosome integrity of frozen-thawed spermatozoa, as well as birth and survival of calves following insemination with frozen-thawed semen of cloned and nuclear donor bull and normal bull. Six ejaculates and 3 samples per ejaculate from each bull were tested, and the Mann-Whitney U test was used to analyse the data. The spermatological parameters of cloned bull semen – volume, concentration, and motility of fresh – were within accepted limits for artificial insemination (4.60 ± 0.47 mL, 1.55 ± 0.21 × 109 spermatozoa mL–1, 80.00 ± 1.07%, respectively). Frozen-thawed sperm motility and viability rate were higher in the cloned bull (56.6%, 56.7%) than in its nuclear donor (47%, 43%; P < 0.05). Intact membrane and DNA fragmentation rate of cloned bull and its nuclear donor bull sperm were similar (P > 0.05) but the intact acrosome rate of cloned bull was higher than that of its nuclear donor (P < 0.05). Low rates in frozen-thawed sperm of nuclear donor can be related to storage time of sperm which were frozen 5 years before. One (clone 4) of the cloned grey heifers was artificially inseminated with frozen semen from nuclear donor bull and the other (clone 5) was naturally mated with a Holstein bull. Two healthy calves were delivered naturally. When same cloned cows (clones 4–5) and 2 other cloned heifers (clones 2–3) were artificially inseminated with frozen semen of the cloned grey bull, clones 2 and 4 gave birth to 2 healthy female calves. One cloned cow (clone 3) aborted in the third month of gestation and other one (clone 5) is currently 8 months pregnant. Two calves of clone 4 and 5 are 17 months old and 2 other calves of clone 2 and 4 are now 6 and 1 months old. Except for clone 3, our results show that cloned Anatolian grey bull and cows produced from frozen cells in gene bank have normal fertility.
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Nair, Sreejith J., A. S. Brar, C. S. Ahuja, S. P. S. Sangha, and K. C. Chaudhary. "A comparative study on lipid peroxidation, activities of antioxidant enzymes and viability of cattle and buffalo bull spermatozoa during storage at refrigeration temperature." Animal Reproduction Science 96, no. 1-2 (November 2006): 21–29. http://dx.doi.org/10.1016/j.anireprosci.2005.11.002.
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Szczesniak-Fabianczyk, B., M. Bochenek, A. T. Palasz, J. De la Fuente, and Z. Smorag. "95 EFFICACY OF FIVE DIFFERENT SEMEN EXTENDERS FOR THE CRYOPRESERVATION OF BULL SEMEN." Reproduction, Fertility and Development 20, no. 1 (2008): 128. http://dx.doi.org/10.1071/rdv20n1ab95.
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Replacement of animal-origin components in extenders used for bull semen freezing is of high importance for individuals involved in cattle breeding. The experiment was designed to compare efficacy of 5 different semen extenders in cryopreservation of bull semen: sodium citrate-based extender containing egg yolk (CT), commercially available Bioxcell� (IMV Technologies, L'Aigle, France), and 3 custom-made homogenized plant lipidsbased, egg yolk-free extenders (Y-1, Y-2, and Lipo) . The objective was to determine whether homogenization procedures of lipids improve the quality of the extender. Lipid homogenates of custom-made extenders were prepared in Tris buffer using a high pressure homogenizer (Nira Saovi, Parma, Italy). Ten (Y-1) or 5 (Y-2) homogenization cycles were applied and then 8% glycerol was added. Lipid liposomes were produced by simultanous high pressure homogenization of lipids and glycerol supplementation (Lipo). Semen was collected from young bulls of 3 different breeds (Simmental, Polish Red, and Holstein; 1 ejaculate/bull). Each ejaculate with at least 70% motility was split into 5 parts and processed further by a standard freezing protocol: semen was diluted at 35�C with each of the 5 extenders to a concentration of 100 � 106 spermatozoa per mL, cooled to 4�C over 5 h, aspirated into 0.25-mL plastic straws, frozen in liquid nitrogen vapor to –140�C, and then plunged into LN2. Straws were thawed in a water bath at 37�C for 20 s. Sperm motility was estimated microscopically immediately after thawing and after 5 h of storage at 22�C. Immediately after thawing, flow cytometry and SYBR-14/PI staining were used for examination of sperm membrane integrity (live/dead assay). A total of 20 000 spermatozoa of each sample were counted. Student's t-test was used to estimate statistical differences between experimental groups. The mean sperm motility after thawing ranged from 45.6% (SD = 13.7) for CT (egg yolk extender) to 57.8% (SD = 7.1) for Lipo. A significant difference (P < 0.05) was observed betweenY-1 (50.0%, SD = 9.7) and Lipo and Bioxcell (56.1%, SD = 8.6). After 5 h of storage at 22�C, the mean motility for all tested bulls ranged from 25.0% (SD = 7.1) for CT to 42.2% (SD = 7.5) for Lipo. Significant differences were observed between Lipo (P < 0.01), Y-2 (P < 0.05) and CT, and between Y-1 and Lipo (P < 0.01). Mean percentage of 'live' spermatozoa with intact membrane after freezing/thawing was 51.85% (SD = 11.49) for Y-1, 45.72% (SD = 9.36) for Y-2, 47.57% (SD = 7.93) for Lipo, 45.47% (SD = 8.35) for Bioxcell, and 49.06 (SD = 11.59) for CT. No significant differences were observed except forY-1 and Bioxcell extenders (P < 0.05). It can be concluded that both methods of lipid/glycerol homogenization can be successfully applied in the preparation of bull semen extender. In addition, extensive lipid homogenization (10 cycles) produced more transparent extender that in turn improved visualization of sperm. Custom-made plant origin lipids homogenization may provide a valuable alternative for the preparation of extenders that more closely match the membrane composition of bull sperm cells and thus contribute to development of an efficient extender free of animal-origin components for bull semen freezing.
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Yekti, Aulia Puspita Anugra, Jois Harsah, Muchamad Luthfi, Muhammad Dikman, Asri Nurul Huda, Kuswati Kuswati, and Trinil Susilawati. "Kualitas Semen dengan Berbagai Formulasi Pengencer Dasar Air Kelapa Hijau Selama Simpan Dingin pada Sapi Madura." Jurnal Ilmu dan Teknologi Peternakan Tropis 5, no. 2 (December 16, 2018): 37. http://dx.doi.org/10.33772/jitro.v5i3.4738.
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ABSTRAKInseminasi Buatan dengan menggunakan semen cair digunakan untuk daerah yang sulit nitrogen cair dan mempunyai kualitas yang lebih baik dari pada semen beku. Tujuan penelitian ini adalah untuk mengetahui kualitas berbagai bahan pengencer dasar air kelapa penyimpanan dingin 2-5°C.Penelitian ini dilaksanakan di Loka Penelitian Sapi Potong Grati, Pasuruan. Semen yang digunakan berasal dari sapi madura sebanyak dua ekor, berumur 5 dan 3 tahun dan berat badan yaitu 397 kg dan 360,5 kg. Sapi madura ditampung seminggu 2 kali dengan motilitas > 70% , sedangkan air kelapa yang digunakan adalah air kelapa hijau yang masih muda. Pengenceran semen cair dibagi menjadi 4 yaitu P0 (CEP-3 + 20% kuning telur) sebagai kontrol, P1 (air kelapa hijau +20% kuning telur), P2 (P1 + 0,4% putih telur + 1% fruktosa) dan P3 (P1 + 0,4% putih telur kuning telur +2% fruktosa). Data dianalisis menggunakan uji Pearson’s Chi Square dan Uji Deskriptif. Hasil penelitian menunjukkan motilitas spermatozoa sesuai standar SNI yaitu motilitas> 40% pada pengencer CEP-3 dapat disimpan selama hari ke-8 (40,50±6,43%) sedangkan pada pengencer dasar air kelapa hijau pada P1, P2 dan P3 tidak menunjukkan perbedaan yang nyata (P>0,05) selama disimpan 6 hari yaitu 40,50±10,12%, 38,00±4,22%, 40,00±8,50%. Abnormalitas dari semua perlakuan menunjukan nilai <20%. Viabilitas didapatkan nilai tertinggi pada perlakuan P0(89,58±2,16%) kemudian P1(89,39±3,79%), P2(88,62±4,59%) dan P3(87,93±4,41%).Kata kunci: CEP-3, semen cair, sapi madura, simpan dingin, air kelapa hijau ABSTRACTArtificial Insemination using liquid semen is performed for areas that where liquid nitrogen is difficult to find and havng better quality than frozen semen. Purpose of this research was to investigate the quality on various coconut water base diluents on liquid semen of madura bull during cold storage of 2-5°C. Research was conducted at Laboratory of Reproduction of Grati Beef Cattle Research Station, Pasuruan.Semen that is used comes from two madura bulls aged 5 and 3 years with body weight is 397 kg and 360.5 kg. The semen was collected twice a week with motility> 70%, and the coconut water used is unripe green coconut water. The research treatments were P0 (CEP-3 + 20% egg yolk) as control, P1 (unripe green coconut water + 20% egg yolk), P2 (P1+ 1% fructose + 0.4% egg white) and P3 (P1+ 0.4% egg white + 2% fructose). Data were analyzed using Pearson's Chi Square test and Descriptive Test. The results showed that the motility of spermatozoa was within Indonesian National Standard (SNI) with more than 40% motility in the CEP-3 diluent and it can be stored until the 8th day (40.50 ± 6.43%). The basic diluents of green coconut water at P1, P2 and P3 was not significantly affected (P> 0.05) until 6 days storing with the motility number average are 40.50 ± 10.12%, 38.00 ± 4.22%, 40, 00 ± 8.50%. The abnormality of all treatments was under 20%. The highest viability was showed by treatment P2 (89.58±2.16%), followed by P4 (89.39 ± 3.79%), P3 (88.62 ± 4.59%) and the lowest was P4 (87.93 ± 4.41%). Keywords:CEP-3, liquid semen, madura bull, cool storage, green coconut water ABSTRACT Artificial Insemination using liquid semen is performed for areas that where liquid nitrogen is difficult to find and havng better quality than frozen semen. Purpose of this research was to investigate the quality on various coconut water base diluents on liquid semen of madura bull during cold storage of 2-5°C. Research was conducted at Laboratory of Reproduction of Grati Beef Cattle Research Station, Pasuruan.Semen that is used comes from two madura bulls aged 5 and 3 years with body weight is 397 kg and 360.5 kg. The semen was collected twice a week with motility> 70%, and the coconut water used is unripe green coconut water. The research treatments were P0 (CEP-3 + 20% egg yolk) as control, P1 (unripe green coconut water + 20% egg yolk), P2 (P1+ 1% fructose + 0.4% egg white) and P3 (P1+ 0.4% egg white + 2% fructose). Data were analyzed using Pearson's Chi Square test and Descriptive Test. The results showed that the motility of spermatozoa was within Indonesian National Standard (SNI) with more than 40% motility in the CEP-3 diluent and it can be stored until the 8th day (40.50 ± 6.43%). The basic diluents of green coconut water at P1, P2 and P3 was not significantly affected (P> 0.05) until 6 days storing with the motility number average are 40.50 ± 10.12%, 38.00 ± 4.22%, 40, 00 ± 8.50%. The abnormality of all treatments was under 20%. The highest viability was showed by treatment P2 (89.58±2.16%), followed by P4 (89.39 ± 3.79%), P3 (88.62 ± 4.59%) and the lowest was P4 (87.93 ± 4.41%).
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Shedova, E. N., G. N. Singina, V. A. Bagirov, and N. A. Zinovieva. "147 THE DEVELOPMENTAL CHARACTERISTICS OF IN VITRO-PRODUCED CATTLE-WISENT (BOS TAURUS-BISON BONASUS) HYBRID EMBRYOS." Reproduction, Fertility and Development 29, no. 1 (2017): 182. http://dx.doi.org/10.1071/rdv29n1ab147.
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Interspecies hybrids are important resources for research and agriculture. Therefore, the aim of this study was to evaluate development, quality, and viability of embryos produced in vitro using cattle (Bos taurus) oocytes and European bison (Bison bonasus) epididymal sperm. The epididymes were obtained following a forced slaughter of one bull aged 7 years. The sperm was collected by scraping the inner surface of the epididymes, diluted with the cryopreservation medium, and equilibrated for 4 h at 4°C. Thereafter, sperm aliquots (0.2 mL) were frozen in liquid nitrogen vapor for 5 min and then plunged into liquid nitrogen for storage. Prior to fertilization, frozen semen was thawed in pre-warmed medium for 1 min at 37°C and prepared by the swim-up method. The frozen-thawed ejaculated sperm from the Russian Black Pied bulls was used as a positive control. Slaughterhouse-derived cumulus-oocyte complexes were matured for 24 h in TCM-199 supplemented with 10% FCS, 0.2 mM sodium pyruvate, 10 μg mL−1 porcine FSH, and 10 μg mL−1 ovine LH. Matured oocytes (35–40 oocytes per group) were co-incubated for 18 h with homologous (n = 266 oocytes) or heterologous (n = 292 oocytes) sperm (spermatozoa/mL) in 500 µL of TALP containing 10 μg mL−1 heparin, 20 μM penicillamine, 10 μM hypotaurine, 1 μM epinephrine, and 0.1% minimal essential medium nonessential amino acids. After IVF, the oocytes were cultured in CR1aa medium (Rosenkrans 1994 J. Anim. Sci. 72, 434–437) to the blastocyst stage. All the cultures were performed at 38.5°C and 5% CO2 in humidified air. At Days 2 and 7 after insemination, the cleavage and blastocyst rates were determined. In addition, a part of obtained blastocysts was fixed with 4% paraformaldehyde, and the total cell number and apoptotic cell ratio were determined by 4’,6-diamidino-2-phenylindole and TUNEL staining. The remaining blastocysts were cultured up to Day 10, and the hatching rates were assessed. The data (3–5 replicates) were analysed by ANOVA. The cleavage rates did not differ among both male species (72.4 and 77.1%). Furthermore, no significant effects of interspecies fertilization on the blastocyst rate or total cell number per blastocyst were found (27.4 ± 1.6% and 77.0 ± 5.7 for cattle embryos and 26.2 ± 1.9% and 83.1 ± 8.9 for cattle-wisent hybrid embryos). On the other hand, the significant differences between homologous and heterologous fertilization were detected in the rate of hatched blastocysts (60.3 ± 5.1 v. 38 ± 2.9, P < 0.05) and apoptotic cell ratio 7.3 ± 0.8 v. 11.6 ± 1.04, P < 0.05). Our findings demonstrate that hybrid embryos produced by IVF of bovine oocytes with the epididymal sperm of European bison can be developed up to advanced blastocyst stages. However, the hybrid embryos have a lower quality and viability than cattle embryos. Research was supported by the Program of Presidium of the Russian Academy of Science, project no. IV.13.3.
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Tethool, Angelina Novita, Gatot Ciptadi, Sri Wahjuningsih, and Trinil Susilawati. "Karakteristik dan Jenis Pengencer Semen Sapi Bali: Suatu Review." Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) 12, no. 1 (April 24, 2022). http://dx.doi.org/10.46549/jipvet.v12i1.214.
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Abstract Bali cattle are Indonesian native cattle that are preferred because they have adaptability and reproductive efficiency capabilities. The productivity of this cow can be increased using artificial insemination. The success of IB implementation is determined by the quality of the semen used. This article aims to provide an overview of the quality of fresh semen, after dilution and the types of diluents that have generally been used to maintain Bali cattle spermatozoa. The quality of fresh semen provides an overview of the condition of the semen after storage. Types of diluents that have been used are chemical diluents such as tris aminomethane, CEP, skim milk, andromed and natural diluents such as coconut water, carrot juice, guava filtrate, honey and tomato juice. Bali cattle semen stored in each diluent gives a different quality response depending on the diluent used. This diluent has a different response to semen qualities in cold and frozen storage. Keywords: Quality of semen; Diluent; Bali cattle; Spermatozoa Abstrak Sapi Bali merupakan sapi asli Indonesia yang disukai karena memiliki kemampuan adaptasi dan kemampuan efisiensi reproduksi. Produktivitas sapi ini dapat ditingkatkan menggunakan inseminasi buatan. Keberhasilan pelaksanaan IB ditentukan oleh kualitas semen yang digunakan. Artikel ini bertujuan untuk memberikan gambaran tentang kualitas semen segar, setelah pengenceran dan jenis pengencer yang umumnya telah digunakan untuk mempertahankan spermatozoa sapi Bali. Kualitas semen segar memberikan gambaran kondisi semen setelah penampungan. Jenis pengencer yang telah digunakan adalah pengencer dari bahan kimia seperti tris aminomethane, CEP, susu skim, andromed dan pengencer alternatif seperti air kelapa, sari wortel, filtrat jambu biji, madu dan sari buah tomat. Semen sapi Bali yang disimpan pada masing-masing pengencer memberikan respon kualitas yang berbeda-beda tergantung bahan pengencer yang digunakan. Secara umum pengencer yang digunakan mampu mempertahankan kualitas semen sapi Bali. Kata kunci: Kualitas semen; Pengencer; Sapi Bali; Spermatozoa
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Mooventhan, P., and Mamta Choudhary. "Assessment of Frozen Semen Quality through Foldscope Microscopy- A Novel Application of Frugal Science to Reduce the Infertility Rate." Indian Journal of Animal Research, Of (February 22, 2022). http://dx.doi.org/10.18805/ijar.b-4699.
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Background: Quality determination of frozen semen before using for Artificial Insemination (AI) in cattle is of paramount importance. The success of cattle AI programs is largely depends on the use of good quality semen and thus assessment of semen quality parameters before AI will improve the conception rate and reduces the loss associated with repeated breeding. Among the various semen quality indicators, sperm motility is one of the important parameter and it should be evaluated immediately after semen collection (before freezing) and 24 hrs after freezing as per minimum standard protocol in frozen semen production. Though the motility is a major determinant of spermatozoa ability to reach the site of fertilization and it is affected by cryopreservation and subsequent poor cold storage facilities, motility is not frequently evaluated under field conditions. In the current scenario, many of our government AI sub-stations in remote villages are not equipped with microscope facility for semen quality evaluation before AI. Methods: In this study, we used foldscope, an origami-based paper microscope which give optical quality similar to conventional microscopes (magnification of 140X and 2-micron resolution), to evaluate the frozen semen motility at field level. In this study, the motility of spermatozoa was observed under foldscope and concluded that foldscope is very useful to asses the motility befor AI and improve the fertility in dairy animals. Result: We revealed that, utility of foldscope to assess the motility of spermatozoa was evaluated under field conditions and observed that 46% of sperms were motile. Further, evalaution of sperm motility before AI is not very common practice due to lack of microscope in many AI centres. Therefore, foldscope will be very useful at field conditions to avoid AI using poor quality semen and subsequent loss associated with repeat breeding problems.