Academic literature on the topic 'Cationic Vector'
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Journal articles on the topic "Cationic Vector":
TAN, AMELIA LI MIN, ALISA XUE LING LIM, YITING ZHU, YI YAN YANG, and MAJAD KHAN. "CATIONIC BOLAAMPHIPHILES FOR GENE DELIVERY." COSMOS 10, no. 01 (December 2014): 25–38. http://dx.doi.org/10.1142/s0219607714400059.
Bengali, Zain, and Lonnie D. Shea. "Gene Delivery by Immobilization to Cell-Adhesive Substrates." MRS Bulletin 30, no. 9 (September 2005): 659–62. http://dx.doi.org/10.1557/mrs2005.193.
Nakayama, Yasuhide, Takeshi Masuda, Makoto Nagaishi, Michiko Hayashi, Moto Ohira, and Mariko Harada-Shiba. "High Performance Gene Delivery Polymeric Vector: Nano-Structured Cationic Star Polymers (Star Vectors)." Current Drug Delivery 2, no. 1 (January 1, 2005): 53–57. http://dx.doi.org/10.2174/1567201052772825.
Porter, Colin D., Katalin V. Lukacs, Gary Box, Yasuhiro Takeuchi, and Mary K. L. Collins. "Cationic Liposomes Enhance the Rate of Transduction by a Recombinant Retroviral Vector In Vitro and In Vivo." Journal of Virology 72, no. 6 (June 1, 1998): 4832–40. http://dx.doi.org/10.1128/jvi.72.6.4832-4840.1998.
Guo, Man, Yingcai Meng, Xiaoqun Qin, and Wenhu Zhou. "Dopamine-Grafted Hyaluronic Acid Coated Hyperbranched Poly(β-Amino Esters)/DNA Nano-Complexes for Enhanced Gene Delivery and Biosafety." Crystals 11, no. 4 (March 29, 2021): 347. http://dx.doi.org/10.3390/cryst11040347.
Marquet, Franck, Viorica Patrulea, and Gerrit Borchard. "Comparison of triblock copolymeric micelles based on α- and ε-poly(L-lysine): a Cornelian choice." Polymer Journal 54, no. 2 (October 13, 2021): 199–209. http://dx.doi.org/10.1038/s41428-021-00552-5.
Budker, Vladimir, Vladimir Gurevich, James E. Hagstrom, Fedor Bortzov, and Jon A. Wolff. "pH-sensitive, cationic liposomes: A new synthetic virus-like vector." Nature Biotechnology 14, no. 6 (June 1996): 760–64. http://dx.doi.org/10.1038/nbt0696-760.
Ito, Akira, Tetsuya Takahashi, Yujiro Kameyama, Yoshinori Kawabe, and Masamichi Kamihira. "Magnetic Concentration of a Retroviral Vector Using Magnetite Cationic Liposomes." Tissue Engineering Part C: Methods 15, no. 1 (March 2009): 57–64. http://dx.doi.org/10.1089/ten.tec.2008.0275.
Natsume, Atsushi, Masaaki Mizuno, Yasushi Ryuke, and Jun Yoshida. "Cationic Liposome Conjugation to Recombinant Adenoviral Vector Reduces Viral Antigenicity." Japanese Journal of Cancer Research 91, no. 4 (April 2000): 363–67. http://dx.doi.org/10.1111/j.1349-7006.2000.tb00953.x.
El-Mahdy, Ahmed F. M., Takayuki Shibata, Tsutomu Kabashima, Qinchang Zhu, and Masaaki Kai. "Delivery of siRNA using siRNA/cationic vector complexes encapsulated in dendrimer-like polymeric DNAs." RSC Advances 5, no. 41 (2015): 32775–85. http://dx.doi.org/10.1039/c5ra01032b.
Dissertations / Theses on the topic "Cationic Vector":
Nouveau, Thibaut. "Nébulisation de nouveaux polyplexes pour le transfert de gènes." Electronic Thesis or Diss., Sorbonne université, 2023. https://accesdistant.sorbonne-universite.fr/login?url=https://theses-intra.sorbonne-universite.fr/2023SORUS734.pdf.
Gene therapy is a form of therapy used to treat hereditary or acquired genetic diseases such as cancer or cystic fibrosis. Introducing a polynucleotide into diseased cells, either via the systmeic route or the local route (oral or nasal inhalation), corrects the defects causing the genetic mutations. However, DNA can only be internalized using a vector that protects it and enables it to reach the cell nucleus, where it will be transcribed. Various vectors (viral or synthetic) have been developed, such as PEI-based cationic polymer vectors. However, although effective, these PEI-based vectors are immunogenic at high doses. Functionalizations to reduce this toxicity, such as PEGylation, have been developed, making it possible to reinforce vectors by adding stealthiness to the final polyplexes. However, these strategies have their limitations, necessitating the synthesis of new types of polymer. POxylation represents a good alternative to PEG usage to form new polyplexes by adding a block formed from one or more poly(2-alkyl-2-oxazoline)s. The copolymers are synthesized by selective hydrolysis of a PEtOx-b-PnPrOx-b-PMeOx triblock copolymer using the thermosensitive properties of the hydrophobic blocks and a kosmotropic salt to form core-shell systems enabling hydrolysis of the PMeOx block to PEI. Then, the systems were formulated using a standard formulation and a "micro-extrusion" method. The polyplexes obtained were used in vitro experiments, by deposition or by a nebulization method, ideal for the treatment of pulmonary diseases. Very good transfection results were obtained, depending on various parameters (Mn, PEI, polymer architecture, +/- charge ratio)
Terp, Megan Cavanaugh. "Improved Nanoparticle Preparation and Delivery Technology for DOTAP and Oligonucleotide Based Lipoplexes." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1338301430.
Ho, J. K. W. "Synthesis and biophysical studies of cationic lipids as gene delivery vectors." Thesis, University College London (University of London), 2012. http://discovery.ucl.ac.uk/1389331/.
Mohammadi, A. "Design and development of tuneable cationic lipopolyplexes as vectors in gene therapy." Thesis, University College London (University of London), 2013. http://discovery.ucl.ac.uk/1389064/.
Belmadi, Nawal. "Développement, formulation et biodistribution de vecteurs synthétiques pour le transfert de gènes dans le cadre de la thérapie génique de la mucoviscidose." Thesis, Brest, 2015. http://www.theses.fr/2015BRES0093/document.
Cystic fibrosis is a monogenic disease characterized by mutations occurring at the CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) gene. The clonining in 1989 of the CFTR gene has enabled to consider treating this disease by gene therapy. This consists of transferring a normal version of the CFTR gene in the affected patients’ cells, using a vector. Due to the severity of pulmonary complications, it is obvious that the respiratory epithelium constitutes the target tissue for the gene transfer. The principle of gene therapy is indeed very attractive and a number of clinical trials have already been made. Gene therapy requires vectorization tools that are efficient and compatible with repeated clinical use.My thesis has focused on the development, biodistribution and optimization of synthetic vectors (cationic lipids) for gene transfer in the respiratory epithelium. During my work, we were able to develop useful fluorescent KLN47 lipophosphoramidates for in vivo biodistribution studies. Compared to non fluorescent KLN47, these new compounds exhibit the same physicochemical properties: a relatively small size and a positive zeta potential. On cell lines, we found that the new formulations were as effective as the KLN47, with little or no toxicity. Then, in animal models, the biodistribution profiles of pegylated and non-pegylated lipoplexes were compared after systemic injection. The biodistribution profiles of pegylated and non-pegylated lipoplexes were similar. However, the pegylation of the complex resulted in prolonged circulation in the bloodstream, whereas transgene expression (luciferase) was equivalent in both cases. In addition, luciferase activity was similar to that obtained with the non-fluorescent KLN47. We have demonstrated that the addition of fluorescent lipid probes in the liposomal solution KLN47, does not change its physicochemical and transfectant properties. The overall results show that we have promising tools for in vivo biodistribution studies. Other molecules have also been tested successfully
Cruz, Claudemir Mota da. "Estudo dos campos vetoriais polinomiais quadráticos que possuem integral primeira racional de grau 3." Universidade Federal da Paraíba, 2011. http://tede.biblioteca.ufpb.br:8080/handle/tede/7354.
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
This work was dedicated to classify all the global phase portraits of the quadratic polynomial vector fields having a rational first integral of degree three. For this, techniques were used as blow-up, classification of singular points, invariant curves for a system of ordinary diferential equations and vector fields induced on the sphere.
Este trabalho foi dedicado a determinação global dos retratos de fase, no disco de Poincaré, dos campos vetoriais polinomiais quadráticos que possuem integral primeira racional de grau três. Para determinar o retrato de fase, utilizamos técnicas como blow-up, classi- ficação dos pontos singulares, curvas invariantes para um sistema de equações diferenciais ordinárias e a indução de campos vetoriais sobre a esfera.
Ver?ssimo, Lourena Mafra. "Desenvolvimento de nanosistemas farmac?uticos para terapia g?nica." Universidade Federal do Rio Grande do Norte, 2011. http://repositorio.ufrn.br:8080/jspui/handle/123456789/12638.
Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior
Gene therapy is one of the major challenges of the post-genomic research and it is based on the transfer of genetic material into a cell, tissue or organ in order to cure or improve the patient s clinical status. In general, gene therapy consists in the insertion of functional genes aiming substitute, complement or inhibit defective genes. The achievement of a foreigner DNA expression into a population of cells requires its transfer to the target. Therefore, a key issue is to create systems, vectors, able to transfer and protect the DNA until it reaches the target. The disadvantages related to the use of viral vectors have encouraged efforts to develop emulsions as non-viral vectors. In fact, they are easy to produce, present suitable stability and enable transfection. The aim of this work was to evaluate two different non-viral vectors, cationic liposomes and nanoemulsions, and the possibility of their use in gene therapy. For the two systems, cationic lipids and helper lipids were used. Nanoemulsions were prepared using sonication method and were composed of Captex? 355; Tween? 80; Spam? 80; cationic lipid, Stearylamine (SA) or 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP) and water (Milli-Q?). These systems were characterized by average droplet size, Polidispersion Index (PI) and Zeta Potential. The stability of the systems; as well as the DNA compaction capacity; their cytotoxicity and the cytotoxicity of the isolated components; and their transfection capacity; were also evaluated. Liposomes were made by hydration film method and were composed of DOTAP; 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), containing or not Rhodaminephosphatidylethanolamine (PE- Rhodamine) and the conjugate Hyaluronic Acid DOPE (HA-DOPE). These systems were also characterized as nanoemulsions. Stability of the systems and the influence of time, size of plasmid and presence or absence of endotoxin in the formation of lipoplexes were also analyzed. Besides, the ophthalmic biodistribution of PE-Rhodamine containing liposomes was studied after intravitreal injection. The obtained results show that these systems are promising non-viral vector for further utilization in gene therapy and that this field seems to be very important in the clinical practice in this century. However, from the possibility to the practice, there is still a long way
A terapia g?nica ? um dos maiores desafios propostos pela pesquisa p?s-gen?mica e se baseia na transfer?ncia de material gen?tico a uma c?lula, tecido ou ?rg?o com o intuito de curar ou melhorar o estado cl?nico do paciente. Em sua forma mais simples, a terapia g?nica consiste na inser??o de genes funcionais em c?lulas com genes defeituosos objetivando substituir, complementar ou inibir esses genes causadores de doen?as. Para que o DNA ex?geno seja expresso em uma popula??o celular faz-se necess?ria a sua transfer?ncia at? o local de a??o. Assim, ? necess?rio criar ve?culos, que transportem e protejam o DNA at? que este chegue a uma popula??o celular alvo. Os obst?culos encontrados com a utiliza??o de vetores virais t?m proporcionado o interesse no desenvolvimento de vetores n?o-virais, por serem f?ceis de produzir, apresentarem estabilidade control?vel e facilitarem a transfec??o g?nica. O objetivo deste trabalho foi avaliar dois diferentes vetores n?o virais, lipossomas e nanoemuls?es cati?nicos, e sua poss?vel utiliza??o na terapia g?nica. Para isso, foram utilizados lip?deos cati?nicos e co-tensoativos na produ??o dos dois sistemas. As nanoemuls?es foram produzidas pelo m?todo de sonica??o e compostas por Captex? 355; Tween? 80; Spam? 80; lip?deo cati?nico, Estearilamina (EA) ou N-[1-(2,3-Dioleoiloxi)propil]-N,N,Ntrimetilamonio metilsulfato (DOTAP); e ?gua ultra-pura (Milli-Q?). Estes sistemas foram caracterizados quanto ao tamanho m?dio de got?cula, ?ndice de polidispers?o (PI) e potencial zeta. Avaliou-se ainda a estabilidade dos sistemas e suas capacidades de compacta??o do material gen?tico. Os lipossomas foram preparados a partir do m?todo de hidrata??o do filme e compostos por DOTAP, Dioleilfosfatidiletanolamina (DOPE), na presen?a ou aus?ncia de Rodaminafosfatidiletanolamina (PE-Rodamina) e do conjugado ?cido Hialur?nico DOPE (HA-DOPE). Estes sistemas foram caracterizados da mesma forma que as nanoemuls?es e tamb?m foram avaliados estabilidade, influ?ncia do tempo, tamanho de material gen?tico e presen?a ou aus?ncia de endotoxinas na forma??o dos lipoplexos. Os resultados obtidos permitem afirmar que os sistemas s?o promissores para posterior utiliza??o na terapia g?nica e que esta ?rea promete ser uma ?rea f?rtil de pesquisa cient?fica e cl?nica por muitos anos, e provavelmente se tornar? uma pr?tica cl?nica importante neste s?culo. No entanto, da possibilidade ? pr?tica existe um longo caminho a percorrer
Le, Bihan Olivier. "Etude par microscopie électronique des mécanismes d'action de vecteurs synthétiques pour le transfert de gènes." Thesis, Bordeaux 1, 2009. http://www.theses.fr/2009BOR13972/document.
The vast majority of clinical trials of gene transfer in vivo use viral vectors. Although they are effective, they induce immunogenic, toxic or mutagenic risks. Due to their high modularity and low toxicity, synthetic vectors (non viral), represent a promising alternative despite their lack of effectiveness. The major objective of this work was to understand the mechanism of gene transfer using two prototypic synthetic vectors, in the context of a rational design of new vectors. We studied on cultured cells, the mechanism of action of two cationic lipids; BGTC (bis(guanidinium)-tren-cholesterol) and DOSP (DiOleylamine A-Succinyl-Paromomycine) formulated with plasmid DNA (lipoplexes) which are in vitro efficient vectors. We have been able to visualize by electron microscopy, their intracellular pathways, their structural alterations and their endosomal escape, the latter being a key step in the process of gene transfer. The unambiguous identification of lipoplexes throughout their intracellular trafficking has been made possible thanks to the labelling of DNA by core-shell silica nanoparticles with an electron dense maghemite core (Fe2O3). The labeling strategy has also been applied to study the mechanism of action of a nonionic block copolymer (P188 or Lutrol). Interestingly, these synthetic vectors have an in vivo transfection efficiency in mice lung and muscle tissue while they are totally inefficient in vitro. We have shown that Lutrol induces an increase of DNA internalization into cells and fails to trigger endosomal escape, which would explain the lack of in vitro efficacy. These findings suggest that the in vivo mechanism of action of Lutrol would involve other internalization pathways
Feng, Tzu-Hua, and 馮子驊. "Synthesis and Characterization of Star-Shaped Cationic Copolymer Hybrid Micelles as a Gene Vector." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/52853289244065649927.
高雄醫學大學
醫藥暨應用化學研究所
100
In this study, amphiphilic star-shaped cationic copolymers were synthesized of hydrophobic monomer and hydrophilic cationic monomer. The synthesized polymer was determined by 1H-NMR spectroscopy and gel permeation chromatography (GPC). The synthesized star-shaped cationic copolymer micelles and hybrid micelles contained PEG are formed by oil-in-water solvent evaporation method. The polyplex binding ability, transfection efficiency and cytotoxicity of micelles are all compared by gel electrophoresis performance, luciferase assay and MTT assay.
Lee, Chien Hsing, and 李建興. "THE STUDY OF POLYCATIONS AND CATIONIC LIPOSOMES AS A NON-VIRAL VECTOR FOR GENE DELIVERY." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/67776008321623946734.
國立臺灣大學
生物化學暨分子生物學研究所
91
During the past years, non-viral gene delivery vector was emerging as a new modality as well as a technology in laboratory and in medical practice. A promising area of research is the design of targeted lipid-DNA complexes. It is clear that the physicochemical properties of lipid-DNA complexes are very important in the design of the ideal lipidic vector. The requirements for such an ideal vector can be summarized as follows: (1) the particles should be small enough to be taken up by organs and surface protected by polyethylene glycol (PEG), to avoid protein interaction in the serum and thus extend their circulation life time into the blood; (2) furthermore, the addition of a targeting ligand is necessary to allow specific recognition by target cells, and (3) the DNA should be condensed and protected from nuclease degradation; (4) finally, DNA should be sufficiently inert to the immune cells to avoid a high level of cytokine production. The results in this report demonstrate the feasibility of improvement in target-specific gene delivery to HER-2-overexpressing cells by insertion of lipid-modified anti-HER-2-Fab’ into the preformed liposomes; in another hand, the large polyethylenimine (PEI) could synergistically increase the transfection efficiency when combined with the cationic liposomes.
Book chapters on the topic "Cationic Vector":
Jubeli, Emile, William P. D. Goldring, and Michael D. Pungente. "Cationic Lipid-Based Nucleic Acid Vectors." In Methods in Molecular Biology, 19–32. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3718-9_2.
Sherly, M. Caroline Diana, S. S. Priya, and M. R. Rekha. "Cationic Polyelectrolyte Vectors in Gene Delivery." In Particulate Technology for Delivery of Therapeutics, 395–417. Singapore: Springer Singapore, 2017. http://dx.doi.org/10.1007/978-981-10-3647-7_12.
Falsini, Sara, and Sandra Ristori. "Lipoplexes from Non-viral Cationic Vectors: DOTAP-DOPE Liposomes and Gemini Micelles." In Methods in Molecular Biology, 33–43. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3718-9_3.
Flechsler, Insa, A. Surovoy, K. Charissé, E. Bayer, and G. Jung. "Comparison of Antisense Vectors and Antisense Oligonucleotides Delivered by Means of the New Cationic Lipids Unifectin and Maxifectin." In Advances in Experimental Medicine and Biology, 469–72. Boston, MA: Springer US, 1998. http://dx.doi.org/10.1007/978-1-4615-5357-1_72.
Arruda, Danielle Campiol, Anne Schlegel, Pascal Bigey, and Virginie Escriou. "Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers." In Methods in Molecular Biology, 137–48. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3718-9_8.
Zahid, Maliha, Xiaoli Lu, Zhibao Mi, and Paul D. Robbins. "Cationic and Tissue-Specific Protein Transduction Domains." In Tissue-Specific Vascular Endothelial Signals and Vector Targeting, Part B, 83–95. Elsevier, 2010. http://dx.doi.org/10.1016/s0065-2660(10)69007-4.
Bulbake, Upendra, Anjali Jain, and Wahid Khan. "Nanocarriers as Non-Viral Vectors in Gene Delivery Application." In Multifunctional Nanocarriers for Contemporary Healthcare Applications, 357–80. IGI Global, 2018. http://dx.doi.org/10.4018/978-1-5225-4781-5.ch013.
"Nomenclature and Classifi cation." In Plant Virus, Vector, 23–30. CRC Press, 2010. http://dx.doi.org/10.1201/9780429196409-6.
Fang, Yang, and Ke Zhang. "Cationic vs. non-cationic polymeric vectors for nucleic acid delivery." In Reference Module in Materials Science and Materials Engineering. Elsevier, 2021. http://dx.doi.org/10.1016/b978-0-12-822425-0.00024-5.
Marshall, John, Nelson S. Yew, Simon J. Eastman, Canwen Jiang, Ronald K. Scheule, and Seng H. Cheng. "Cationic Lipid-Mediated Gene Delivery to the Airways." In Nonviral Vectors for Gene Therapy, 39–68. Elsevier, 1999. http://dx.doi.org/10.1016/b978-012358465-6/50015-3.
Conference papers on the topic "Cationic Vector":
Ito, Akira, Tetsuya Takahashi, Yujiro Kameyama, Yoshinori Kawabe, and Masamichi Kamihira. "Magnetic Manipulation of a Retroviral Vector Using Magnetite Cationic Liposomes." In 2008 International Symposium on Micro-NanoMechatronics and Human Science (MHS). IEEE, 2008. http://dx.doi.org/10.1109/mhs.2008.4752479.
Naruki, M., H. En’yo, R. Muto, T. Tabaru, S. Yokkaichi, Y. Fukao, H. Funahashi, et al. "Medium Modi cation on Vector Mesons Observed in 12 GeV p + A Reactions." In INTERSECTIONS OF PARTICLE AND NUCLEAR PHYSICS: 9th Conference CIPAN2006. AIP, 2006. http://dx.doi.org/10.1063/1.2402687.
Reports on the topic "Cationic Vector":
Ohad, Itzhak, and Himadri Pakrasi. Role of Cytochrome B559 in Photoinhibition. United States Department of Agriculture, December 1995. http://dx.doi.org/10.32747/1995.7613031.bard.