Journal articles on the topic 'Casa isolata'
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Haryani, Fitri, Aliefman Hakim, and Nisa Isneni Hanifa. "Perbandingan Pelarut Etanol 96% dan Aseton pada Ekstraksi dan Isolasi Kurkuminoid dari Rimpang Kunyit." Lumbung Farmasi: Jurnal Ilmu Kefarmasian 2, no. 2 (July 5, 2021): 112. http://dx.doi.org/10.31764/lf.v2i2.5493.
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ABSTRAKKunyit (Curcuma longa Linn.) secara empiris digunakan masyarakat Bima sebagai obat infeksi kulit bernanah (antibakteri) karena kandungan kurkuminoidnya. Kurkuminoid dapat diperoleh dengan cara isolasi. Penelitian ini bertujuan untuk melakukan isolasi kurkuminoid dengan metode yang sederhana menggunakan dua pelarut berbeda dan mengetahui rendemen isolat hasil isolasi. Langkah awal dalam proses isolasi yang dimodifikasi, yaitu proses ekstraksi selama 30 menit menggunakan sonikator dengan pelarut aseton dan etanol 96%, serta perbandingan bobot simplisia dan volume pelarut sebesar 1:10. Proses isolasi dilanjutkan dengan ekstraksi padat-cair menggunakan pelarut n-heksan, kemudian dimurnikan dengan metode kristalisasi menggunakan campuran isopropil alkohol panas : n-heksan (1 : 1,5). Isolat dianalisis secara kualitatif dengan kromatografi lapis tipis (KLT) dan dihitung bobot isolat sampel. Hasil yang diperoleh berupa Nilai Rf untuk standar kurkumin, sampel pelarut aseton dan sampel pelarut etanol 96% adalah 0.81, 0.80 dan 0.81. Rendemen ekstrak etanol 96% dan aseton berturut-turut sebesar 27,3% dan 26,44%. Bobot isolat etanol 96% dan aseton sebesar 0,07 g dan 0,035 g. Isolasi kurkuminoid dengan metode sederhana menggunakan pelarut etanol 96% menghasilkan bobot isolat lebih besar dibanding pelarut aseton.Kata kunci: Modifikasi isolasi; Kurkuminoid; Kunyit.ABSTRACTTurmeric (Curcuma longa Linn.) is empirically used by the people of Bima as a purulent skin infection (antibacterial) drug because of its curcuminoid content. Curcuminoid can be obtained by isolation. This study aims to isolate curcuminoid with a simple method using two different solvents and determine the yield of isolated isolates. The first step in the isolation process is the extraction process for 30 minutes using a sonicator with acetone and 96% ethanol as solvent, and the ratio of simplicia weight and solvent volume is 1:10. The process was continued with solid-liquid extraction using n-hexane as solvent, then purified by crystallization method using a mixture of hot isopropyl alcohol: n-hexane (1 : 1.5). The isolates were analyzed qualitatively by thin layer chromatography (TLC) and calculated the weight of the sample isolate. The results obtained in the form of Rf values for standard curcumin, acetone solvent samples and 96% ethanol solvent samples were 0.81, 0.80 and 0.81. The yield of ethanol extract 96% and acetone were 27.3% and 26.44%, respectively. The weight of 96% ethanol and acetone isolates was 0.07 g and 0.035 g, respectively. Isolation of curcuminoid with a simple method using 96% ethanol as a solvent resulted in a higher weight of isolate than acetone solvent.Keyword: Isolation modification; Curcuminoid; Turmeric.
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Mahardhika, Wahyu Aji, Gendis Angger Pitaloka, Rizky Nurcahyo, Isworo Rukmi, and Agung Suprihadi. "Isolation and Identification of Entomopathogenic Fungi from Teak Leaf Little Soil and Paddy Soil with Various Isolation Methods." Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) 9, no. 2 (November 6, 2022): 23–30. http://dx.doi.org/10.23960/jbekh.v9i2.228.
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Abstrak Abstract Kapang entomopatogen merupakan kapang yang memiliki kemampuan untuk menginfeksi serangga. Kapang tersebut dapat menginfeksi dengan cara penembusan hifa ke tubuh serangga inang melalui kulit, saluran pencernaan, spirakel dan lubang lainnya. Tujuan dari penelitian ini yaitu untuk mengisolasi dan mengidentifkasi kapang entomopatogen pada tanah serasah daun jati dan tanah sawah dengan metode isolasi yang berbeda. Metode isolasi kapang entomopatogen menggunakan metode insect bait, insect bait dengan pengayakan tanah, dan metode pengenceran. Serangga yang digunakan untuk uji larva Tenebrio molitor. Isolasi tanah sawah berhasil menumbuhkan kapang entomopatogen pada tubuh larva Tenebrio molitor ditandai dengan adanya miselium kapang menutupi tubuh larva. Hasil isolasi didapatkan 6 isolat yaitu S2A, S2B, S2C, S3A, S3B, S3C. Keenam isolat tersebut merupakan kapang Fusarium sp. Kata kunci: Fusarium sp., identifikasi, isolasi, kapang Entomopatogen, T. molitor Entomopathogenic fungi are molds that have the ability to infect insects. The mold can infect by penetrating the hyphae into the host insect's body through the skin, digestive tract, spiracles, and other holes. The purpose of this study was to isolat and identified entomopathogenic molds in teak leaf litter and paddy fields with different isolation methods. The isolation method of entomopathogenic molds used the insect bait method, insect bait with soil sieving, and the dilution method—insects used for the larval test of Tenebrio molitor. Isolation of paddy fields succeeded in growing entomopathogenic molds on the larval body of Tenebrio molitor, which was indicated by the presence of mold mycelium covering the larval body. The isolation results obtained 6 isolats, namely S2A, S2B, S2C, S3A, S3B, S3C. The six isolats were Fusarium sp. Keywords: Entomopathogenic fungi, Fusarium sp., Identification, Isolation, T. molitor
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Li, Ming, Rui Wang, Dahe Zhao, and Hua Xiang. "Adaptation of the Haloarcula hispanica CRISPR-Cas system to a purified virus strictly requires a priming process." Nucleic Acids Research 42, no. 4 (November 20, 2013): 2483–92. http://dx.doi.org/10.1093/nar/gkt1154.
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Abstract The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas system mediates adaptive immunity against foreign nucleic acids in prokaryotes. However, efficient adaptation of a native CRISPR to purified viruses has only been observed for the type II-A system from a Streptococcus thermophilus industry strain, and rarely reported for laboratory strains. Here, we provide a second native system showing efficient adaptation. Infected by a newly isolated virus HHPV-2, Haloarcula hispanica type I-B CRISPR system acquired spacers discriminatively from viral sequences. Unexpectedly, in addition to Cas1, Cas2 and Cas4, this process also requires Cas3 and at least partial Cascade proteins, which are involved in interference and/or CRISPR RNA maturation. Intriguingly, a preexisting spacer partially matching a viral sequence is also required, and spacer acquisition from upstream and downstream sequences of its target sequence (i.e. priming protospacer) shows different strand bias. These evidences strongly indicate that adaptation in this system strictly requires a priming process. This requirement, if validated also true for other CRISPR systems as implied by our bioinformatic analysis, may help to explain failures to observe efficient adaptation to purified viruses in many laboratory strains, and the discrimination mechanism at the adaptation level that has confused scientists for years.
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Marliani, Lia, Dadang Juanda, and Arif Rubianto. "Isolation of Antioxidant Compounds from Ethanol Extract of Temu Kunci (Boesenbergia pandurata Roxb.) Rhizomes." Acta Pharmaceutica Indonesia 38, no. 2 (June 28, 2013): 48–51. http://dx.doi.org/10.5614/api.v38i2.5209.
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Temu kunci (Beosenbergia pandurata Roxb.) is traditionally used to treat various diseases, and antioxidants are one of their utilization. The aim of this study was to isolate and identify the antioxidant compounds of temu kunci rhizomes. Temu kunci rhizomes was extracted by maceration with ethanol 95%. Etanol extract was then fractionated by liquid-liquid extraction, vacuum liquid chromatography, and classical column chromatography. Monitoring and testing the antioxidant activity used thin-layer chromatography (TLC) with 0.2% DPPH (1,1-diphenyl-2-picrylhydrazyl) reagent. Purity test was performed by a single development TLC using three different kinds of mobile system and two-dimensional TLC. Isolate BP-1 was isolated from the ethanol extract and active as an antioxidant. Based on ultraviolet-visible and infrared spectrums, isolate BP-1 was identified as flavanone in the absence of hydroxyl groups at the ortho position (o-diOH), with substitution of -OH at C5 and C7.Keywords: Temu kunci, Boesenbergia pandurata, DPPH, Antioxidant. AbstrakTemu kunci (Beosenbergia pandurata Roxb.) secara tradisional digunakan untuk mengobati berbagai macam penyakit, salah satunya adalah sebagai antioksidan. Tujuan dari penelitian ini adalah untuk mengisolasi dan mengidentifikasi komponen antioksidan yang dimiliki temu kunci. Rimpang temu kunci diekstraksi dengan cara maserasi menggunakan etanol 95%. Ekstrak etanol kemudian difraksinasi dengan ekstraksi cair-cair, kromatografi cair vakum, dan kromatografi kolom klasik. Aktivitas antioksidan kemudian dipantau dan diuji menggunakan kromatografi lapis tipis (KLT) menggunakan reagen 0,2% DPPH (1,1-diphenyl-2-picrylhydrazyl). Uji kemurnian dilakukan dengan pengembangan tunggal KLT menggunakan 3 macam fase gerak yang berbeda dan KLT dua-dimensi. Isolat BP-1 merupakan hasil isolasi dari ekstrak etanol dan aktif sebagai antioksidan. Berdasarkan spektrum UV-Vis dan inframerah, isolat BP-1 teridentifikasi sebagai flavanone karena adanya gugus hidroksil yang hilang pada posisi orto (o-diOH), dengan substitusi "“OH pada C5 dan C7.Kata kunci : Temu kunci, Boesenbergia pandurata, DPPH, antioksidan.
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Akhsan, Ni’matuljannah, Surya Sila, and Tifa Esta Noviana. "ISOLASI JAMUR ENTOMOPATOGEN PADA LAHAN TANAMAN PANGAN, HORTIKULTURA, DAN PERKEBUNAN DI KABUPATEN PENAJAM PASER UTARA DAN UJI PATOGENISITAS PADA Spodoptera litura." Agrifor 21, no. 2 (October 4, 2022): 265. http://dx.doi.org/10.31293/agrifor.v21i2.6038.
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Jamur entomopatogen merupakan jenis bioinsektisida yang dapat digunakan untuk mengendalikan hama pada tanaman. Pengendalian menggunakan bioinsektisida yang berbahan aktif jamur entomopatogen merupakan salah satu cara pengendalian yang ramah lingkungan serta tidak berdampak negatif bagi lingkungan dan tidak merusak ekosistem disekitarnya. Tujuan dari penelitian ini adalah untuk mengidentifikasi jamur entomopatogen yang terdapat pada lahan tanaman pangan, hortikultura, dan perkebunan dan mengetahui tingkat patogenisitas jamur tersebut sebagai entomopatogen pada Spodoptera litura. Lokasi pengambilan sampel berada di Saloloang, Kabupaten Penajam Paser Utara, Kalimantan Timur. Penelitian ini dilaksanakan di Laboratorium Hama Penyakit Tanaman Fakultas Pertanian, Universitas Mulawarman, pada bulan Agustus 2020 hingga Maret 2021. Perlakuan menggunakan isolat jamur entomopatogen yang berhasil di isolasi dengan insect bait method menggunakan larva Tenebrio molitor. Uji patogenisitas dirancang dalam Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan. Data dianalisis menggunakan sidik ragam (ANOVA) dan uji lanjut menggunakan uji beda nyata terkecil (BNT) pada taraf 5%. Hasil penelitian ini ditemukan 4 jenis jamur entomopatogen yang di isolasi di Kabupaten Penajam Paser Utara yaitu Aspergillus sp. isolat lahan tanaman perkebunan, Cunninghamella sp. isolat lahan tanaman hortikultura, Fusarium sp. isolat lahan tanaman pangan dan perkebunan, dan Metarhizium sp. isolat lahan tanaman pangan, hortikultura, dan perkebunan. Semua isolate jamur Metarhizium sp. efektif mengendalikan ulat grayak (Spodoptera litura) in vitro dengan kerapatan spora 105/mL.
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SWANTARA, I. MADE DIRA, WIWIK SUSANAH RITA, and ANISA HERNINDYA. "Identifikasi Isolat Antikanker Spons Hyrtios Erecta." Indonesian Journal of Cancer 10, no. 4 (September 19, 2017): 123. http://dx.doi.org/10.33371/ijoc.v10i4.456.
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ABSTRACTIsolation, anticancer activity test, and identification of the toxic isolate from ethanol extract of the sponge Hyrtios erecta taken from Pari Island beach (Jakarta) has conducted. Extraction of the sponges was carried out by 70% ethanol at room temperature. Partition and purification of the compounds were done by column chromatography with the stationary phase of silica gel and the mobile phase of n-hexane-chloroform (2:8). Toxicity screening test was done based on BhrineShrimp Lethality Test (BSLT). In vitro anticancer activity test of the isolate was carried out using HeLa cell line. Identification of the compounds was performed by Gas chromatography-mass spectroscopy (GC-MS). Based on theresults, it was found that the toxic isolate of H. erecta sponges has anticancer activity with IC50 of 30,497 ppm. Four compounds was detected from the anticancer isolate i.e: 4-nonylphenol; dibutyl phthalate; hexanedioic acid bis(2-ethylhexyl) ester; and cholesterol. ABSTRAKTelah dilakukan isolasi, uji aktivitas antikanker, dan identifikasi isolat toksik yang berasal dari ekstrak etanol spons Hyrtios erecta yang diambil dari perairan Pulau Pari (Jakarta). Ekstraksi dilakukan dengan cara maserasi menggunakan etanol 70% pada temperatur kamar. Pemisahan dan pemurnian komponen menggunakan kromatografi kolom dengan fase diam silikagel dan fase gerak n-heksana-kloroform (2:8). Skrining toksisitas dilakukan dengan metode Bhrine Shrimp Lethality Test (BSLT). Uji antikanker secara in vitro isolat toksik tersebut menggunakan sel HeLa. Senyawanya diidentifikasi menggunakan Gas chromatography-mass spectroscopy (GC-MS). Berdasarkan hasil penelitian ini diperoleh bahwa isolat toksik spons H. erecta bersifat antikanker dengan IC50 sebesar 30,497 ppm. Pada isolat antikankertersebut terdeteksi empat senyawa, yaitu 4-nonylphenol; dibutil phtalat; ester heksadioat bis(2-etilheksil); dan kolesterol.
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Sariadji, Kambang, and Sunarno Sunarno. "Toksigenitas Corynebacterium diphtheriae Pada Sampel Kejadian Luar Biasa Difteri Tahun 2010 – 2015 Menggunakan Elektes." Jurnal Kesehatan Andalas 6, no. 1 (July 20, 2017): 208. http://dx.doi.org/10.25077/jka.v6i1.672.
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Strain Corynebacterium diphtheriae dapat dibedakan berdasarkan sifat toksigenisitas atau kemampuannya dalam memproduksi toksin difteri. Jenis C.diphtheriae yang mampu memproduksi toksin disebut jenis toksigenik, sedangkan yang tidak memproduksi toksin disebut jenis non-toksigenik. Diagnosis laboratorium dalam mendeteksi C.diphtheriae dilakukan dengan cara kultur, isolasi dan identifikasi serta penentuan toksigenitas dari difteri. Tujuan penelitian ini adalah menentukan toksigenitas C.diphtheriae pada isolat hasil investigasi kejadian luar biasa antara tahun 2010 sampai 2015. Sebanyak 61 Isolat C.diphtheriae ditumbuhkan dan diidentifikasi kembali, kemudian dilakukan uji toksigenitas dengan cara elektes. Hasil pemeriksaan kultur dan isolasi menunjukkan 57 isolat adalah C.diphtheriae dengan berbagai sub tipe dan 4 isolat adalah Corynebacterium pseudodiphthericum yang merupakan bakteri bersifat komensal di saluran pernapasan atas dan kulit manusia. Hasil elektes dari 57 isolat menunjukkan 54 isolat C.diphtheriae adalah toksigenik dan 3 isolat non toksigenik. Simpulan studi ini ialah isolat C.diphtheriae hasil investigasi KLB didapatkan 54 isolat toksigenik dan 3 isolat non toksigenik.
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Irawati, Wahyu. "Amplifikasi Gen Resistensi Tembaga (Cur) pada Bakteri yang Diisolasi dari Limbah Industri di Surabaya." Jurnal Teknologi Lingkungan 21, no. 1 (January 30, 2020): 16–22. http://dx.doi.org/10.29122/jtl.v21i1.3274.
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ABSTRACTCopper pollution is one of the serious environmental problems in Indonesia. Higher concentration of copper is toxic so that threat living organism. Bio-remediation using copper resistant bacteria is effective for solving heavy metals pollution because the bacteria adapt easily when applied in environment. Using bacteria containing gene encoded copper resistance could help effort of copper bio-remediation. The purpose of this research is to isolate and characterize copper resistant bacteria from industrial sewage in Surabaya also to amplification of gene encoded resistance to copper (Cur). Bacteria was grown on Salts Base Solution medium with addition of appropriate concentration of copper. Resistance to copper was determined based on Minimum Inhibitory Concentration of CuSO4. Molecular characterization was done based on 16S rDNA gene analysis using universal primer. Amplification of Cur gene was done using specific primer of gene orf3, orf4, orf5 from Lactoccocus lactis. Three highly copper resistant bacteria have been isolated with the MIC of 2.5-7.5 mM CuSO4 encoded IrC1, IrC2, and IrC4. Isolate IrC4 is the highest copper resistant bacteria with the MIC of 7.5 mM. Resistance mechanism may be through accumulation copper inside the cells with the total of 371 mg/g dry weight of cells. The three bacteria have plasmid with the size of 21 kb. Isolate IrC4 have 96.99% similarity with Cupriavidus pauculus. Amplification of copper-resistance (Cur) gene demonstrated that a single band of 0.9 kb was obtained from isolate C4. The finding of indigenous resistant bacteria encoded Cur gene may give better solution for pollution problem in Indonesia.Keywords: bacteria, copper, Isolate IrC4, Lactococcus lactis, resistantABSTRAKPencemaran tembaga di Indonesia merupakan salah satu masalah lingkungan yang serius sehingga perlu diatasi. Tembaga pada konsentrasi yang tinggi bersifat toksik sehingga mengancam kehidupan organisme. Bioremediasi menggunakan bakteri resisten tembaga yang diisolasi dari lingkungan tercemar terbukti efektif mengatasi pencemaran logam berat karena lebih mudah beradaptasi ketika diterapkan di lingkungan. Pemanfaatan bakteri yang mengandung gen penyandi resistensi tembaga dapat menunjang keberhasilan usaha bioremediasi tembaga. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi bakteri resisten tembaga dari limbah industri di Surabaya serta melakukan amplifikasi gen yang menyandi resistensi bakteri tersebut terhadap tembaga (Cur). Isolasi bakteri dilakukan menggunakan medium Salt Base Solution dengan penambahan CuSO4. Uji resistensi ditentukan berdasarkan nilai Minimum Inhibitory Concentration (MIC) terhadap CuSO4. Karakterisasi molekular dilakukan berdasarkan analisis gen 16S rDNA menggunakan primer universal. Amplifikasi gen Cur dilakukan dengan menggunakan primer yang spesifik terhadap gen orf3, orf4, orf5 dari gen Cur pada Lactobacillus lactis. Hasil penelitian menunjukkan bahwa terdapat tiga isolat bakteri yang paling resisten dengan nilai MIC= 6.5-7.5 mM, yaitu isolat IrC1, IrC2, dan IrC4. isolat IrC4 merupakan isolat bakteri yang paling resisten dengan nilai MIC sebesar 7.5 mM. Mekanisme reistensi isolat IrC4 adalah dengan cara mengakumulasi tembaga di dalam sel selitar 371 mg/g berat kering sel. Ketiga isolat memiliki plasmid berukuran sekitar 21 kb. Analisis gen 16S rDNA menunjukkan bahwa isolat IrC4 memiliki kemiripan 96,99% dengan Cupriavidus pauculus. Gen Cur pada isolat IrC4 sepanjang 0.9 kb berhasil diamplifikasi dengan menggunakan primer spesifik gen orf3 yang menyandikan pengikatan tembaga. Penemuan bakteri indigen indonesia yang menyandikan gen resisten tembaga diharapkan dapat menunjang keberhasilan penanganan masalah pencemaran tembaga di Indonesia.Kata kunci: bakteri, isolat IrC4, Lactobacillus lactis, resisten, tembaga.
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Supriyadi, Hambali, Taukhid Taukhid, Ani Widiyati, and Desy Sugiani. "EFEKTIVITAS ANTIBIOTIKA DAN VAKSIN DALAM PENANGGULANGAN PENYAKIT STREPTOCOCCOSIS PADA IKAN NILA (Oreochromis niloticus)." Jurnal Riset Akuakultur 1, no. 1 (November 15, 2016): 61. http://dx.doi.org/10.15578/jra.1.1.2006.61-65.
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Penelitian dengan tujuan untuk mengetahui antibiotik yang efektif untuk pengobatan penyakit streptococcosis, serta mendapatkan cara pencegahan penyakit secara biologis yaitu melalui penggunaan vaksin telah dilakukan di Laboratorium Riset kesehatan Ikan Pasar Minggu. Tiga jenis antibiotika yaitu Neomycin, Oxytetracyclin, dan Enrofloxacin diuji efektivitasnya terhadap 4 isolat bakteri Streptococcus iniae yaitu Y2N7, Y2N9, GM2.4, dan S1N8 melalui uji zona hambatan dan konsentrasi hambat minimum (MIC). Uji imunogenitas diuji dengan cara pembuatan vaksin dari isolat yang digunakan yang kemudian dievaluasi level titer antibodi yang diproduksi. Hasil penelitian menunjukkan bahwa enrofloxacin merupakan antibiotik yang efektif terhadap semua isolat yang diuji, sedangkan neomycin efektif hanya untuk isolat Y2N7. Isolat GM2.4 relatif memiliki sifat immunogenitas lebih baik dibanding dengan isolat uji lainnya.Research with the aims to evaluate the effectiveness of several antibiotics against 4 (four) streptococcus iniae isolates, and evaluation of immunogecity of those isolate to be used for disease control (vaccine) have been conducted at Fish Health Research Laboratory Pasar Minggu. The effectiveness of three antibiotics namely Neomycin, Oxytetracyclin, and Enrofloxacin have been tested against 4 (four) isolates Y2N7, Y2N9, GM2.4, and S1N8. The immunogenicity of those isolates were also tested by developing vaccine and evaluated through the production of antibody titer level. The results indicated that enrofloxacin was effective against all isolates tested, meanwhile neomycin only effective against isolate Y2N7. Isolate of GM2.4 was relatively immunogenic as compared to the other isolates.
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Matte, Willian Daróz, Rubem Silvério de Oliveira Jr., Fellipe Goulart Machado, Jamil Constantin, Denis Fernando Biffe, Francisco De Souza Dias Gutierrez, and João Renato Vaz da Silva. "Eficácia de [atrazine + mesotrione] para o controle de plantas daninhas na cultura do milho." Revista Brasileira de Herbicidas 17, no. 2 (July 13, 2018): 587. http://dx.doi.org/10.7824/rbh.v17i2.587.
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Para aumentar o espectro de controle das plantas daninhas na cultura do milho, a formulação de herbicidas com princípios ativos de diferentes mecanismos de ação é uma alternativa importante. Objetivou-se avaliar o controle promovido pelo novo herbicida Calaris®, mistura formulada de [atrazine+mesotrione] aplicado em pós-emergência das plantas daninhas, de forma isolada ou em mistura com outros herbicidas. Os experimentos foram conduzidos em casa de vegetação, um para cada planta daninha avaliada, em delineamento inteiramente casualizado com 16 tratamentos e quatro repetições. As sementes das plantas daninhas (Conyza bonariensis, Urochloa decumbens, Cenchrus echinatus, Digitaria horizontalis, Urochloa plantaginea, Ipomoea grandifolia, Euphorbia heterophylla, Bidens pilosa, Glycine max e Commelina benghalensis) foram semeadas em vasos com volume de 5 dm3 a uma densidade de 20 sementes por vaso. Os tratamentos herbicidas foram aplicados em pós-emergência e foram constituídos por [atrazine+mesotrione] isolado ou em mistura com glyphosate, tembotrione e nicosulfuron; atrazine isolado ou em mistura com glyphosate, mesotrione, tembotrione e nicusulfuron; glyphosate isolado e testemunha sem aplicação. A aplicação dos tratamentos de cada experimento foi realizada em datas distintas, levando em consideração o estádio de desenvolvimento das plantas daninhas. Avaliou-se a porcentagem de controle em relação às plantas daninhas aos 7, 14 e 28 dias após a aplicação dos herbicidas. A nova formulação herbicida [atrazine+mesotrione] controlou de forma eficaz a maioria das plantas daninhas avaliadas, exceto Cenchrus echinatus e Urochloa decumbens, entretanto, o controle eficaz foi obtido quando em associação com outros herbicidas, principalmente ao glyphosate.
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Mirsam, Hishar, Masluki Masluki, and Mutmainnah Mutmainnah. "Isolasi dan Seleksi Cendawan Rhizosfer dan Endofit asal Tanaman Kelor sebagai Agens Penginduksi Perkecambahan pada Benih Padi." AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian 5, no. 1 (May 28, 2021): 34–43. http://dx.doi.org/10.33019/agrosainstek.v5i1.227.
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Cendawan rhizosfer dan endofit merupakan jenis mikroba fungsional yang mampu memproduksi metabolit sekunder yang dapat memengaruhi pertumbuhan tanaman baik secara langsung atau tidak langsung. Penelitian ini bertujuan mengisolasi dan menguji kemampuan cendawan rhizosfer dan endofit asal tanaman kelor terhadap viabilitas dan vigor kecambah benih padi. Eksplorasi cendawan dilakukan terhadap sampel tanah di bagian rhizosfer serta jaringan batang dan daun tanaman kelor sehat. Isolasi cendawan dari tanah bagian rhizosfer dilakukan dengan teknik pengenceran 10-2 dan 10-3, sedangkan cendawan endofit dilakukan pada jaringan daun dan batang tanamn kelor, kemudian dibiakkan pada media medium potato dextrose agar (PDA). Uji patogenisitas cendawan dan pengaruhnya terhadap perkecambahan benih padi secara in-vitro dengan metode blotter test, yaitu dengan cara menumbuhkan benih padi sebanyak 25 butir pada isolat cendawan berumur 7 hari. Sebanyak sembilan belas isolat cendawan berhasil diisolasi dan dikoleksi dari berbagai bagian tanaman kelor. Pengamatan patogenisitas menunjukkan terdapat lima isolat cendawan yang berpotensi sebagai patogen, yaitu isolat RF2, RF5, RF6, RF8, dan EDF6. Sebanyak empat isolat cendawan yang telah diuji konsisten memberikan pengaruh positif terhadap viabilitas dan vigor benih benih dengan nilai ≥90% yaitu isolate RF4, EDF1, EDF2, dan EDFbt3
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Riyanti, Soraya, Clara Sunardi, and Sana Nurul Falah. "Isolation of Flavonoid from Jackfruit Leaves (Artocarpus heterophyllus Lamk.) and its Antioxidant Activity." Acta Pharmaceutica Indonesia 38, no. 2 (June 28, 2013): 58–61. http://dx.doi.org/10.5614/api.v38i2.5211.
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The flavonoid compound was successfully isolated from jackfruit leaves (Artocarpus heterophyllus Lamk.). Extraction was done by maceration with methanol-water (9:1), followed by methanol-water (1:1) as a solvent. Hydrolyzed extract was obtained by adding of 2NHCl, then extracted with ethyl acetate and monitored by thin layer chromatography (TLC) with nhexane-ethyl acetate (3:5) as a mobile phase. Separation was performed by column chromatography, followed by preparativethin layer chromatography. Purity of isolates was determined by 2-dimensional thin layer chromatography. Characterization of isolate were carried out by ultraviolet-visible spectrophotometry and infrared spectrophotometry. The result showed a maximum absorbance of bands I at 328.4 nm and 271 nm for bands II. Characterization of isolate by infrared spectrophotometry showed the presence of functional groups OH, CH, C = C, C = O, CC, and CO. Based on the data, the isolate identified as a flavonoid compound. Antioxidant activity of ethyl acetate fraction and column fraction by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) assay showed EC50 at 60.53 mg/mL and 35.27 μg/mL respectively.Key words: Artocarpus heterophyllus Lamk., flavonoid, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), EC50. AbstrakSenyawa flavonoid berhasil diisolasi dari daun belimbing (Artocarpus heterophyllus Lamk.). Ekstraksi dilakukan dengan cara maserasi menggunakan metanol-air (9:1), diikuti dengan metanol-air (1:1) sebagai pelarut. Hasi dari ekstrak yang terhidrolisis didapatkan dengan menambahkan 2NHCl, kemudian diekstraksi dengan etil asetat dan diamati menggunakan kromatografi lapis tipis (KLT) dengan campuran n-heksana-etil asetat (3:5) sebagai fase gerak. Pemisahan dilakukan menggunakan kromatografi kolom yang dilanjutkan dengan kromatografi lapis tipis preparative. Kemurnian dari isolat ditentukan dengan cara kromatografi lapis tipis 2-dimensi. Karakterisasi isolat ditunjukkan dengan spektrofotometer UV-Vis dan inframerah. Hasil spektrum menujukkan adanya absorbansi maksimum dari pita I pada 328,4 nm dan 271 nm untuk pita II. Karakterisasi dari isolat menggunakan spektrofotometer inframerah menunjukkan adanya gugus fungsional OH, CH, C=C, C=O, CC, dan CO. Berdasarkan data tersebut, isolat teridentifikasi sebagai senyawa flavonoid. Aktivitas antioksidan dari fraksi etil asetat dan fraksi kolom menggunakan pengujian 1,1-difenil-2-pikril-hidrazil (DPPH) menunjukkan EC50 pada 60,53 mg/ml dan 35,27 μg/mL.Kata kunci : Artocarpus heterophyllus Lamk., flavonoid, 1,1-difenil-2-pikril-hidrazil (DPPH), EC50
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Wiraputra, Hady, Marline Nainggolan, and Panal Sitorus. "Senyawa Saponin Hasil Isolasi dari Daun Buni (Antidesma bunius (L.) Spreng.)." Talenta Conference Series: Tropical Medicine (TM) 1, no. 1 (October 2, 2018): 264–70. http://dx.doi.org/10.32734/tm.v1i1.79.
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Tanaman buni (Antidesma bunius (L.) Spreng.) secara tradisional telah digunakan untuk hipertensi, takikardia, anemia, sifilis, antikanker, antioksidan, sumber pewarna alami dan antidiabetes. Saponin merupakan senyawa fitokimia yang mempunyai kemampuan membentuk busa dan mengandung aglikon polisiklik yang berikatan dengan satu atau lebih gula. Penelitian ini bertujuan untuk melakukan karakterisasi senyawa saponin hasil isolasi dari daun buni dengan spektrofotometer ultraviolet dan inframerah. Simplisia daun buni dilakukan karakterisasi kemudian diekstraksi dengan cara maserasi bertingkat menggunakan pelarut n-heksana dan etanol 80%. Selanjutnya ekstrak etanol dihidrolisis dengan HCl 2N kemudian difraksi dengan pelarut kloroform. Isolasi dilakukan terhadap fraksi kloroform dengan cara kromatografi lapis tipis preparatif menggunakan fase diam silika gel GF254 dan fase gerak yang sesuai. Isolat yang diperoleh diuji kemurnian dengan KLT 2 arah dan dikarakterisasi menggunakan spektrofotometer ultraviolet dan inframerah. Hasil pemeriksaan karakterisasi simplisia diperoleh kadar air 7,32%, kadar sari larut dalam etanol 52,70%, kadar sari larut dalam air 23,25%, kadar abu total 6,86% dan kadar abu tidak larut dalam asam 0,94%. Pemisahan fraksi kloroform dengan KLT menggunakan fase gerak n-heksana-etilasetat perbandingan 5:5 diperoleh noda 13 dan hasil KLT preparatif diperoleh 2 isolat murni yaitu isolat 1 (ungu merah) dengan Rf 0,92 dan isolat 2 (biru) dengan Rf 0,78. Hasil karakterisasi isolat 1 diperoleh panjang gelombang maksimum pada 208 nm dan dijumpai adanya gugus hidroksil, gugus -CH alifatis, ikatan C=C, gugus –CH2, gugus –CH3, dan gugus C-O. Hasil karakterisasi isolat 2 diperoleh panjang gelombang maksimum pada 204 nm dan adanya gugus hidroksil, gugus -CH alifatis, gugus –CH2, gugus –CH3, dan gugus C-O.
Buni (Antidesmabunius (L.) Spreng.) has been traditionally used for the treatment of hypertension, tachycardia, anemia, syphilis, and used asanti-cancer, anti-oxidant, natural dye, and anti-diabetic. Saponin is a phytochemical compound which has capability in forming foam and contains polycyclic aglycone that binds with one or more glucose. This research aimed to conduct the characterization of saponin compound from buni leaves with ultraviolet spectrophotometer and infrared. Buni leaves simplicia was characterizedand extracted using sequential maceration method with n-hexane and 80% ethanol. The ethanol extract was hydrolyzed with HCl 2N and fractionized using chloroform solvent. Isolation of chloroform fraction was done using preparative thin-layer chromatography using silent phase of silica gel GF 254 and suitable mobile phase. Isolates obtained was taken into purity test with two dimensions thin-layer chromatography and characterized using ultraviolet spectrophotometer and infrared. The characterized simplicia resulted with 7.32% of water content, 52.70% of dissolved content in ethanol, 23.25% of dissolvedcontent in water, 6.86% of total ash content, and 0.94% of undissolved ash content in acid. Fractinationof chloroform fraction with thin-layer chromatography using mobile phase ofn-hexane-ethyl acetate with 5:5 ration resulted with 13 spotsand the result of the preparative thin-layer chromatography resulted 2 pure isolates which are isolate 1 (purple-red) with Rf 0.92 and isolate 2 (blue) with Rf 0.78. The characterization of isolate 1 resulted that the maximum wave lengthwas 208 nm with hydroxyl group, –CH aliphatic group, C=C bond, –CH2 group, –CH3 group, and C–O group. The characterization of isolate 2 resulted that the maximum wave lengthwas204 nm with hydroxyl group, –CH aliphatic group, –CH2 group, –CH3 group, and C–O group.
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Carvallo-Ochoa, Juan Pablo. "LA MODERNIZACIÓN DE LA CASA BURGUESA EN CUENCA: ESTRATEGIAS DE PROYECTO EN LA CASA PEÑA (1954) Y LA CASA VÁZQUEZ (1962)." DISEÑO ARTE Y ARQUITECTURA, no. 10 (June 10, 2021): 181–95. http://dx.doi.org/10.33324/daya.v1i10.384.
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Históricamente, la casa se ha constituido como el espacio fundamental que permite la realización de las actividades domésticas, la interrelación familiar y el desarrollo y afianzamiento del yo personal. Diversos autores concuerdan que durante el siglo XX suceden las mayores alteraciones en las estructuras sociales, entornos de ciudades y en la vivienda y sus espacios (Cañar & Torres, 2018); convirtiéndose esta, en el laboratorio de experimentación teórico y de aplicación práctica de los modernos estilos de vida (Añón, 2013). En el Ecuador, las transformaciones sociales, económicas y políticas de inicios de siglo, se dieron paralelamente a las transformaciones tecnológicas y energéticas, las cuales con mayor o menor demora llegaban a Cuenca. La presente investigación plantea estudiar la modernización de la casa burguesa en Cuenca, a partir de la identificación y análisis de las estrategias proyectuales aplicadas, en la casa Peña (1954) y la casa Vázquez (1962), por el Arq. Cesar Burbano Moscoso. Estas viviendas se caracterizaron por la innovación y búsqueda de una nueva manera de habitar, asumiendo los cambios que la ciudad exigió a mediados del siglo XX. En los dos casos se evidencia como la tipología tradicional de casa con patio interior, organizada centralmente y alineada y conectada a la calzada, se invierte y muta en una tipología radicalmente contraria, compuesta por construcciones aisladas y retiradas de la calle, modificando así las relaciones, internas de la casa y con la ciudad. El estudio explora procesos arquitectónicos, enfocando el interés en reconocer criterios y valores que provienen de las obras, así como elementos arquitectónicos y urbanos de un momento particular de la arquitectura cuencana. Palabras clave: Arquitectura moderna, vivienda moderna, transformaciones del espacio doméstico, Cesar Burbano Moscoso, Cuenca-Ecuador.
AbstractHistorically, the house has been constituted as the fundamental space that allows the realization of domestic activities, family interrelationship and the development and strengthening of the personal self. Several authors agree that during the twentieth century the greatest alterations in social structures, city environments and housing and its spaces took place (Cañar & Torres, 2018); becoming the laboratory of theoretical experimentation and practical application of modern lifestyles (Añón, 2013). In Ecuador, the social, economic and political transformations at the beginning of the century were parallel to the technological and energy transformations, which with greater or lesser delay reached Cuenca. This research proposes to study the modernization of the bourgeois house in Cuenca, based on the identification and analysis of the applied project strategies, in the Peña House (1954) and the Vázquez House (1962), by the architect Cesar Burbano Moscoso. These houses have been characterized by innovation and the search for a new way of living, assuming the changes that the city demanded in the mid-twentieth century. In both cases it is evident how the traditional typology of a house with an interior patio, centrally organized, aligned and connected to the road, is inverted and transformed into a radically opposite typology, composedof isolated structures and withdrawn from the street, thus modifying the internal relations to the house and with the city. The study explored architectural processes, approaching the interest in recognizing criteria and values that come from the works, as well as architectural and urban elements of a particular moment of Cuenca architecture.
Keywords: Modern architecture, modern housing, transformations of domestic space, Cesar Burbano Moscoso, Cuenca-Ecuador
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Mastura, Mastura, Tonel Barus, Lamek Marpaung, and Partomuan Simanjuntak. "Isolation and Antioxidant Activity of Phenolic Compounds from Halban Leaves (Vitex pinnata Linn) in Aceh." Elkawnie 6, no. 2 (December 30, 2020): 213. http://dx.doi.org/10.22373/ekw.v6i2.5532.
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Abstract: An isolation and identification of phenolic compounds from Halban Leaves (Vitex pinnata Linn), a traditional medicinal plant in Aceh, has been done. Halban leaves were extracted through methanol maceration followed by isolation. The methanol extract went into n-hexane and ethyl acetate partition consecutively resulting in 3 different fractions of ethyl acetate and n-hexane extract. The ethyl acetate fraction demonstrated the most promising antioxidant and cytotoxic activity. Therefore, purification of ethyl acetate fraction was done by column chromatography [SiO2; (i) n-hexane-ethyl acetate 10:1, 1:1 ; (ii) n-hexane-ethyl acetate = 2 : 1); (iii) n-hexane-acetone = 1:1]. The pure isolate as identified by using UV, IR, NMR, and MS spectra afforded 2 phenolic compounds, namely (1) 4-hydroxymethyl benzoate and (2) p-hydroxymethyl benzoic acid (PHBA). The antioxidant activity of compound 1 and cytotoxicity activity of 2 expressed in IC50 and LD50 was 41.08 ppm and 59.41 ppm, respectively.Abstrak: Isolasi dan identifikasi senyawa fenolik dari Daun Halban (Vite xpinnata Linn) tanaman obat tradisional asal Aceh telah dilakukan. Isolasi ini dilakukan dengan cara mengekstraksi daun halban dengan cara maserasi menggunakan pelarut metanol, Ekstrak metanol kemudian dipartiisi dengan n-heksan dan etilasetat secara berturut-turut sehingga diperoleh tiga ekstrak yaitu ekstrak etil asetat, n-heksan dan air. Ekstrak etil asetat memiliki bioaktivitas sebagai antioksidan dan juga toksisitas. Pemurnian dilanjutkan dengan cara kromatografi kolom [SiO2; (i) n-heksan-etilasetat 10:1, 1:1 ; (ii) n-heksan-etilasetat = 2 : 1); (iii) n-heksan-aseton = 1:1] dan diperoleh isolat murni. Isolat murni tersebut diidentifikasi berdasarkan interpretasi data spektra UV, IR, NMR dan MS. Hasil interpretasi data menunjukkan daun halban (V. pinnata Linn) mengandung dua senyawa fenolik yaitu (1) 4-hidroksi metil benzoat dan (2) asam para hidroksi benzoat (PHBA). Senyawa 1 memiliki aktivitas antioksidan dengan nilai IC50 = 41,08bpj dan senyawa 2 memiliki aktivitas toksisitas dengan nilai LC50 = 59,41.
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Sunaryanto, Rofiq, Bambang Marwoto Irawadi, Tun Tedja Irawadi, Zainal Alim Mas’ud, and Liesbetini Hartoto. "Isolasi dan Elusidasi Struktur Kimia Antimikroba yang Dihasilkan Oleh Aktinomisetes Laut." Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan 5, no. 1 (June 19, 2010): 11. http://dx.doi.org/10.15578/jpbkp.v5i1.422.
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Isolasi senyawa aktif dari aktinomisetes laut yang memiliki aktivitas antimikroba telah dilakukan. Isolasi aktinomisetes dilakukan dengan pengenceran dan praperlakuan sampel dengan cara pemanasan dan pengasaman. Sampel diambil dari sedimen laut di 6 titik lokasi Pantai Anyer, Banten. Dari total 29 isolat yang diperoleh, isolat A32 merupakan isolat terpilih untuk penelitian lebih lanjut. Isolat A32 memiliki aktivitas antimikroba terhadap Staphylococcus aureus ATCC25923 dan Candida albican BIOMCC00122. Elusidasi struktur kimia menggunakan ESI-LCMS, 1 HNMR, dan COSY menunjukkan bahwa senyawa aktif tersebut memiliki bobot molekul 501,2 g/mol dan rumus molekul C26H35N3O7. Diduga senyawa ini termasuk dalam golongan makrolakton virginiamycin yaitu Madumycin I.
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Gomes, DNF, MAQ Cavalcanti, MJS Fernandes, DMM Lima, and JZO Passavante. "Filamentous fungi isolated from sand and water of "Bairro Novo" and "Casa Caiada" beaches, Olinda, Pernambuco, Brazil." Brazilian Journal of Biology 68, no. 3 (August 2008): 577–82. http://dx.doi.org/10.1590/s1519-69842008000300016.
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Fungi comprehend a heterogeneous group of heterotrophic microorganisms that act as saprobes or parasites or, less frequently, as symbionts living in association with other organisms. They are cosmopolitan and important components of ecosystems. Considering the small number of Brazilian papers on the filamentous mycota in marine environments, and the need to improve knowledge of the diversity of these microfungi in "Casa Caiada" and "Bairro Novo" beaches, Olinda, PE, the aim of this work was to isolate and identify the fungi from sand and water samples of these ecosystems. Thirty two samplings of sand (surface and 20 cm deep) and water (surface and 1 m deep) were carried out during the dry and rainy seasons, in low and high tide. From each sand sample, a suspension was made with 50 g of sand diluted in 90 mL of sterilized distilled water. From each sand suspension and water sample, 0.5 mL was spread, in triplicate, onto Petri dishes containing Sabouraud Agar added of chloramphenicol. The plates were incubated at 28 °C (±2 °C). Fifty seven species were isolated, identified, and classified in 20 genera. Aspergillus and Penicillium were the most frequent genera in both sand and water, with a total of 11 and 19 species, respectively.
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Silveira, Solimar Dutra da, Virgínia Pagliochi Prates, Eloy Henrique Pares Curuci, Rogério Rodrigues Santos, and Olicies da Cunha. "Isolated lateral meniscus rupture in dogs: case report." Acta Veterinaria Brasilica 14, no. 3 (September 24, 2020): 156–59. http://dx.doi.org/10.21708/avb.2020.14.3.9280.
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Wahyuno, Dono, Dyah Manohara, and Dwi N. Susilowati. "Variasi Morfologi dan Virulensi Phytophthora capsici Asal Lada." Buletin Plasma Nutfah 13, no. 2 (October 7, 2016): 70. http://dx.doi.org/10.21082/blpn.v13n2.2007.p70-81.
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<p>Phytophthora capsici is the most important plant parasitic fungus causes stem rot disease in black pepper cultivation in Indonesia. The objective of the present study was to observe morphological variation and virulence of Phytophthora isolated on black pepper from various areas in Indonesia. Fifty isolates of Phytophthora were observed under light microscope. The observed morphological characteristics of each isolate, i.e. sporangiophore branching type, colony type, mating type and shape of sporangium after they were grown in growth medium of V8 juice agar, while length and width of sporangium, length of sporangiophore, and papilla were measured by micrometer. The variation of their virulence was observed by inoculating the hypha of each isolate on detached leaves of black pepper that incubated in damped boxes in room conditions. The width of necrotics were measured with leaf area meter after incubated for four days. The results indicated, those morphological characteristics of the isolates were vary in size, shape, colony pattern, mating type and sporangiophore branching pattern, which those characteristics were belong to P. capsici. Those morphological characteristics were not related with the mating type, isolated plant parts and its geographic distribution. The virulence of the tested isolates were also vary from low to high, and their virulence were also not related with the mating type, isolated plant parts and its geographic distribution.</p><p> </p><p><strong>Abstrak</strong></p><p>Phytophthora capsici merupakan cendawan penyebab penyakit busuk pangkal batang yang paling banyak menimbulkan kerusakan pada tanaman lada di Indonesia. Penelitian ini bertujuan untuk mengamati variasi morfologi dan virulensi Phytophthora yang diperoleh dari berbagai lokasi pertanaman lada di Indonesia. Setelah ditumbuhkan pada media V8 jus agar, sebanyak 50 isolat Phytophthora diamati karakteristik morfologinya di bawah mikroskop cahaya, yang meliputi tipe percabangan tangkai sporangium, tipe koloni, tipe kawin, dan bentuk sporangium. Pengukuran panjang dan lebar sporangium, tangkai sporangium, dan papilla dilakukan menggunakan micrometer. Variasi virulensi yang ada diamati dengan cara menginokulasikan potongan hifa dari setiap isolat pada helaian daun lada, kemudian diinkubasi di dalam kotak yang lembab dan diletakkan di suhu ruang. Setelah diinkubasi selama empat hari, luas nekrose yang terjadi diukur menggunakan leaf area meter. Hasil pengamatan menunjukkan adanya variasi karakterisitik morfologi dari setiap isolat pada tipe koloni, tipe kawin, percabangan sporangium, ukuran, dan bentuk sporangium. Semua isolat menunjukkan karakteristik P. capsici. Variasi morfologi yang ada tidak berkaitan dengan tipe kawin, asal bagian tanaman yang diisolasi, dan geografi asal isolat. Lima puluh isolat P. capsici juga bervariasi virulensinya, dari rendah sampai tinggi. Variasi virulensi tersebut juga tidak berkaitan dengan tipe kawin, asal bagian tanaman yang diisolasi maupun geografi asal isolat.</p>
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Metelski, Michele. "PROTEGENDO A CASA DOS OPERÁRIOS BRANCOS: AS PRÁTICAS ODONTOLÓGICAS DOS PELOTÕES DE SAÚDE." Poíesis Pedagógica 15, no. 2 (September 15, 2018): 82. http://dx.doi.org/10.5216/rpp.v15i2.47689.
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Esta pesquisa é parte integrante da Tese de Doutorado em andamento desenvolvida na Universidade do Estado de Santa Catarina e objetiva preencher uma lacuna existente na História da Educação. O estudo procura reconstituir a história dos Pelotões da Saúde que foram constituídos na Escolas de Santa Catarina a partir do ano de 1941 demonstrando o lugar dessas instituições e o trabalho que as crianças desenvolviam nas escolas primárias. A pesquisa se baseou nos documentos como Atas, Relatórios Anuais, Relatórios Mensais e Reuniões Pedagógicas localizados no Arquivo Público do Estado de Santa Catarina. O presente texto enfatiza as criações dos pelotões de saúde, que faziam parte das Associações Auxiliares das Escolas, contando inicialmente em 75 escolas no Estado, entre elas, Grupos Escolares, Escolas Mistas Municipais, Escolas Mistas Estaduais, Escolas Estaduais, Escolas Isoladas Mistas Municipais, Escolas Isoladas Mistas Estaduais, Escolas Mistas Desdobradas, Escolas Femininas Estaduais, Escolas Isoladas Estaduais, Colégios Particulares, Escolas Públicas Estaduais.
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Cengiz, Hüseyin, Murat Ekin, and Cihan Kaya. "Isolated fallopian tube torsion: A rare case." Cumhuriyet Medical Journal 35, no. 4/Supplement (December 30, 2013): 27–30. http://dx.doi.org/10.7197/1305-0028.1727.
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Kumar, Dr Santosh. "Isolated Umbilical Cord Cyst: A Case Report." Journal of Medical Science And clinical Research 04, no. 11 (November 4, 2016): 13616–17. http://dx.doi.org/10.18535/jmscr/v4i11.15.
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Richhariya, Dr Aayush. "Isolated Gall Bladder Agenesis – A Rare Case." Journal of Medical Science And clinical Research 05, no. 04 (April 30, 2017): 21100–21104. http://dx.doi.org/10.18535/jmscr/v5i4.215.
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Aziz Ali, Ayesha, Kelsey Musselman, Marie Smithgall, and Kristen Pepin. "A unique case of isolated vulvar endometriosis." Journal of Case Reports and Images in Obstetrics and Gynecology 8, no. 2 (September 6, 2022): 4–6. http://dx.doi.org/10.5348/100124z08aa2022ci.
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Kondreddy, Dr Suhas, Dr Lavanya Sanaboina, Dr Rigved Nittala, and Dr Vidya Kiran B. "Isolated Hemifacial Hypertrophy-A Rare Case Report." International Journal of Scientific Research 3, no. 6 (June 1, 2012): 292–93. http://dx.doi.org/10.15373/22778179/june2014/95.
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Singh, Gurcharan, Lalith Sagar K, and Naveen Kumar H R. "A case of isolated nasal bone osteomyelitis." JOURNAL OF CLINICAL AND BIOMEDICAL SCIENCES 04, no. 2 (June 15, 2014): 286–88. http://dx.doi.org/10.58739/jcbs/v04i2.1.
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Fischer, Svante. "The solidus hoard of Casa delle Vestali in context." Opuscula. Annual of the Swedish Institutes at Athens and Rome 7 (November 2014): 107–27. http://dx.doi.org/10.30549/opathrom-07-05.
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In this paper, I discuss the context of a Late Roman solidus hoard found in the Casa delle Vestali on the Forum Romanum in Rome. The hoard consists of 397 solidi, Late Roman gold coins. Most of the hoard consists of uncirculated solidi struck in the name of the Western Roman emperor Procopius Anthemius (AD 467–472). By means of situating the hoard within the context of the reign of Anthemius and the collapse of the Western Roman Empire, the aim of this paper is to determine if the coins in the Vestal hoard can be related to other contemporary coin hoards by means of numismatic typology; this information could add to our understanding of why Anthemius’ reign is considered such an unmitigated failure and why the Empire collapsed soon after his murder. In this article, the composition of the hoard is examined, and the contents are compared to other contemporary solidus hoards in the Mediterranean, Gaul, Poland and Scandinavia. I argue that this comparison shows that the Vestal hoard is not part of a larger network but that the hoard constitutes the remains of an isolated occurrence—as initially suggested by its unusual composition and location.
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Azizah, Siti Nur. "ISOLASI DAN KARAKTERISASI BAKTERI SELULOLITIK ASAL JERAMI PADI DI PERSAWAHAN BOGOR BARAT." JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER 2, no. 1 (January 28, 2021): 19–28. http://dx.doi.org/10.53864/jifakfar.v2i1.18.
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Kandungan selulosa pada jerami padi tergolong tinggi yaitu sekitar 35%. Sebagai senyawa yang paling melimpah di dunia, selulosa dari jerami padi dapat digunakan sebagai sumber penghasil bakteri selulolitik yang dapat menghasilkan enzim selulase sebagai katalis yang bermanfaat dalam bidang industri, pertanian dan kesehatan. Tujuan dari penelitian ini adalah untuk mendapatkan isolat bakteri selulolitik asal jerami padi di Desa Kampung Jawa Kelurahan Setigede Kecamatan Bogor Barat dengan cara isolasi dan karakterisasi isolat yang didapat pada medium CMC agar. Hasil isolasi pada media CMC agar diperoleh sebanyak 5 isolat bakteri selulolitik yaitu isolat J1, J2, J3,J4 dan J5. Hasil skring aktivitas selulolik secara semikuantitatif menggunakan metode congo red menunjukkan bahwa isolat J2 memiliki nilai IAE tertinggi yaitu 1,84. Hasil karakterisasi secara fisik yaitu makroskopis dan mikroskopis serta karakterisasi secara biokimia sesuai petunjuk Bergey’s Manual of systematic bacteriology menunjukkan bahwa isolat J2 memiliki kemiripan dengan Bacillus sp.Kata kunci: Bakteri selulolitik, jerami padi, karakterisasi bakteri
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Yelli, Fitri, Inggar Damayanti, and Maria Viva Rini. "OPTIMASI VOLUME BUFFER EKSTRAKSI DALAM MENGISOLASI DNA SPESIES MIKORIZA ARBUSKULAR UNTUK IDENTIFIKASI SECARA MOLEKULER." Jurnal Agrotek Tropika 10, no. 3 (July 21, 2022): 381. http://dx.doi.org/10.23960/jat.v10i3.4955.
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Fungi Mikoriza Arbuskular (FMA) mampu meningkatkan pertumbuhan tanaman dengan cara membantu penyerapan air dan unsur hara dari dalam tanah. Selain itu, FMA juga berperan dalam meningkatkan ketahanan tanaman terhadap serangan patogen. Laboratorium Produksi Tanaman Perkebunan Fakultas Pertanian Universitas Lampung memiliki 35 koleksi isolat mikoriza. Isolat-isolat koleksi tersebut belum teridentifikasi dengan baik sampai ke level spesies. Penelitian ini bertujuan untuk menguji empat volume buffer ekstraksi InstaGeneTM Matrix yang efektif dan ekonomis untuk isolasi DNA FMA yang akan digunakan untuk identifikasi secara molekuler. Isolasi DNA 4 isolat FMA yaitu Glomus sp., Gigaspora sp., Acaulospora sp., dan Entrophospora sp. dilakukan dengan menggunakan buffer ekstraksi Instagene Matrix (BIO-RAD) dengan volume 10, 15, 20, dan 25 μl. Untuk melihat keberhasilan isolasi DNA yang dilakukan yaitu dengan menggunakan DNA tersebut sebagai template atau cetakan dalam proses PCR. Hasil penelitian menunjukkan bahwa semua volume (10, 15, 20, dan 25 μl) buffer ekstraksi yang diujikan mampu mengekstrak DNA dari ke 4 isolat yang diuji kecuali volume 20 μl pada isolat Gigaspora sp. Oleh karena itu, volume 10 μl buffer ekstraksi sudah dapat digunakan dan lebih efisien untuk mengekstrak DNA dari spora FMA.
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Rahmi, Narzani, Eni Harmayani, Umar Santosa, and Purnama Darmadji. "Identifikasi Bakteri Asam Laktat dan Aktivitas Penghambatan Radikal pada Jaruk Tigarun (Crataeva nurvala, Buch Ham) (Identification of Lactic Acid Bacteria and Radical Scavenging Activity in Jaruk Tigarun (Crataeva nurvala, Buch HAM))." Jurnal Agritech 36, no. 03 (December 21, 2016): 317. http://dx.doi.org/10.22146/agritech.16604.
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Jaruk tigarun is a traditional fermented food from South Borneo made from tigarun flower that was fermented in water without salt addition. The objectives of the research were to isolate and identify the Lactic Acid Bacteria (LAB) from jaruk tigarun then to determine the DPPH radical scavenging activities of jaruk tigarun extracts. LAB was isolated from jaruk tigarun which was fermented for 7 days at room temperature. Media of MRS+CaCO3 were used to isolate the LAB while API 50 CHL kit was utilised to identify them. Fresh flowers and jaruk tigarun were also freeze-dryed, crushed and extracted using methanol, ethanol and ethyl acetate. The total phenolic and antioxidant activity of each extract were determine with Folin-Ciocalteu method and DPPH. Three isolates of LAB were isolated and identified as Lactobacillus plantarum. The fermentation was able to increase total phenolic of jaruk tigarun which was extracted with methanol, ethanol, and ethyl acetate (53.24 ± 0.73, 44.86 ± 0.90 and 23.95 ± 0.13 mg GAE/g extract, respectively). Similarly, the antioxidant activity of jaruk tigarun that were extracted with methanol, ethanol and ethyl acetate increased to 92.68 ± 0.02 %, 92.43 ± 0.11 %, and 42.94 ± 0.02 %. Methanolic extract of jaruk tigarun has the highest IC50 that was equal to 1.511 μg/mL. UV-Vis spectrum analysis and FT-IR were used to identify the compounds isolated from methanolic extract of jaruk tigarun resulting flavonoid as tentative identified compounds. ABSTRAKJaruk tigarun adalah salah satu makanan fermentasi tradisional dari Kalimantan Selatan yang dibuat dengan cara merendam bunga tigarun dalam air matang selama beberapa hari. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri asam laktat yang terlibat selama fermentasi jaruk serta mengetahui aktivitas penghambatan radikal DPPH pada jaruk tigarun. BAL diisolasi dari bunga tigarun yang difermentasi selama 7 hari pada suhu kamar sampai menjadi jaruk. Isolasi BAL menggunakan media MRS+CaCO3 dan diidentifikasi dengan API 50 CHL Kit. Bunga segar dan jaruk tigarun juga masing-masing dikeringbekukan, dihaluskan dan diekstraksi dengan metanol, etanol dan etil asetat. Total fenolik dan aktivitas antioksidan dari masing masing ekstrak ditentukan dengan metoda Folin Ciocalteu dan DPPH. Tiga isolat BAL berhasil diisolasi dan diidentifikasi sebagai Lactobacillus plantarum. Fermentasi berhasil meningkatkan total fenolik dari jaruk tigarun yang diekstrak dengan metanol, etanol dan etil asetat berturut-turut sebesar 53,24 ± 0,73, 44,86 ± 0,90 dan 23,95 ± 0,13 mg GAE/g ekstrak. Demikian pula halnya dengan aktivitas antioksidan dari jaruk yang diekstrak dengan metanol, etanol dan etil asetat meningkat menjadi 92,68 ± 0,02 %, 92,43 ± 0,11 %, dan 42,94 ± 0,02 %. Ekstrak metanol dari jaruk tigarun memiliki IC50 yang paling baik yaitu sebesar 1.511 μg/mL. Hasil isolasi dan identifikasi senyawa yang dimurnikan dari ekstrak metanol jaruk menggunakan analisis spektrum UV-Vis dan FT-IR diperoleh dugaan senyawa adalah golongan flavonoid.Kata kunci: DPPH; flavonoid; jaruk tigarun; L. Plantarum; total fenolik
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Oliveira, Anderson Ramos de, Silvério de Paiva Freitas, and Ismael Lourenço de Jesus Freitas. "Eficiência de trifloxysulfuron-sodium no controle de Cyperus rotundus L. na cultura da cana-de-açúcar." Revista Ceres 57, no. 6 (December 2010): 736–41. http://dx.doi.org/10.1590/s0034-737x2010000600006.
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O objetivo foi avaliar a eficiência de trifloxysulfuron-sodium isolado, em mistura, ou em aplicação sequencial a outros herbicidas no controle de Cyperus rotundus L. na cultura da cana-de-açúcar. O estudo foi realizado em área de produção comercial do cultivar RB 72-454 na Usina Paraíso e em casa de vegetação da Universidade Estadual do Norte Fluminense Darcy Ribeiro, em Campos dos Goytacazes, Rio de Janeiro. As parcelas no campo foram submetidas a nove tratamentos: 2,4-D; trifloxysulfuron-sodium + ametryn; trifloxysulturon-sodium; 2,4-D + 2,4-D (20 dias após a primeira aplicação -DAP); 2,4-D + trifloxysulfuron-sodium + ametryn (20 DAP); 2,4-D + trifloxysulfuron-sodium (20 DAP); halosulfuron (20 DAP) e testemunha com e sem capina. Em casa de vegetação avaliou-se a viabilidade dos bulbos de C. rotundus em função dos tratamentos utilizados no experimento instalado em campo. Os resultados indicaram que o trifloxysulfuron-sodium isolado ou em mistura com ametryn, em aplicação sequencial ao 2,4-D, foi excelente no controle de C. rotundus. Esse herbicida e o halosulfuron, aplicados isoladamente, reduziram a capacidade de reinfestação da espécie daninha em razão da redução de sua propagação e do seu crescimento.
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Bendre, Dr Mahendra, Dr Nikhil Rane, and Dr Nandkishor Narwade. "Isolated Tuberculosis of Gall Bladder : A Case Report." Indian Journal of Applied Research 4, no. 4 (October 1, 2011): 439–40. http://dx.doi.org/10.15373/2249555x/apr2014/135.
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Rajdeo, Roma N., Ravi N. Rajdeo, A. P. Bakshi, and A. V. Shrikhande. "Isolated Tuberculosis of Gallbladder: A rare case report." Annals of Pathology and Laboratory Medicine 5, no. 1 (2018): C6—C8. http://dx.doi.org/10.21276/apalm.1607.
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Fior, Claudimar Sidnei, Paulo Vitor Dutra de Souza, and Sergio Francisco Schwarz. "Emergência de plântulas de Butia odorata (Barb. Rodr.) Noblick em casa de vegetação." Revista Árvore 37, no. 3 (June 2013): 503–10. http://dx.doi.org/10.1590/s0100-67622013000300013.
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Butia odorata é uma palmeira nativa do Rio Grande do Sul, Brasil, cujos frutos são utilizados na alimentação humana. O objetivo deste estudo foi testar a emergência de plântulas de B. odorata em casa de vegetação a partir de sementes e diásporos submetidos a diferentes procedimentos de superação de dormência. A escarificação através da abertura da cavidade embrionária das sementes permitiu emergência de 72%, com tempo médio de 56 dias. A imersão dos diásporos em água por 18 h antes da semeadura favoreceu a emergência, porém o tempo médio atrasou para 323 dias. Sementes isoladas do endocarpo, porém não escarificadas, apresentaram 38% de emergência, com tempo médio de 319 dias. A emergência a partir de diásporos submetidos a 40 ºC por três semanas antes da semeadura não diferiu da emergência de sementes isoladas do endocarpo; no entanto, o tempo médio foi reduzido de 319 para 206 dias. A abertura da cavidade embrionária possibilitou o maior percentual de emergência de plântulas e menor tempo médio de emergência.
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Töfoli, J. G., S. C. Mello, and R. J. Domingues. "Efeito do fosfito de potássio isolado e em mistura com fungicidas no controle da requeima do tomateiro." Arquivos do Instituto Biológico 79, no. 2 (June 2012): 201–8. http://dx.doi.org/10.1590/s1808-16572012000200008.
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Três experimentos foram realizados, um em casa-de-vegetação e laboratório e os demais em campo, com o objetivo de estudar a ação do fosfito de potássio isolado e em mistura com fungicidas no controle de Phytophthora infestans do tomateiro. A aplicação de 300 g 100 L-1 de fosfito de potássio associado com mancozebe foi mais eficiente no controle da requeima nos cultivares 'Rebeca' e 'Giuliana', em relação à aplicação isolada desses produtos, no quarto e sétimo dia após a inoculação em discos foliares. No campo, clorotalonil/metalaxil-M + mancozebe/propamocarbe associados com fosfito de potássio nas doses de 100, 200 e 300 g 100 L-1 foram mais eficientes no controle da requeima que o cimoxanil + mancozebe/fenamidona/iprovalicarbe + propinebe e o uso isolado de fosfito de potássio. O uso de dimetomorfe + mancozebe + fosfito de potássio reduziu drasticamente a severidade da doença quando comparado à aplicação isolada de mancozebe, de fosfito de potássio ou da mistura mancozebe + fosfito de potássio. A mistura entre mancozebe e fosfito de potássio (300 g 100 L -1) proporcionou maior controle da requeima em relação ao uso isolado.
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Oliveira, Jean Eduardo de, Nilva Kazue Sakomura, Adriana Nogueira Figueiredo, Jorge de Lucas Júnior, and Tânia Mara Baptista dos Santos. "Efeito do isolamento térmico de telhado sobre o desempenho de frangos de corte alojados em diferentes densidades." Revista Brasileira de Zootecnia 29, no. 5 (October 2000): 1427–34. http://dx.doi.org/10.1590/s1516-35982000000500021.
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O objetivo deste trabalho foi avaliar o efeito do uso de isolante térmico de telhado instalado como forro sob a cobertura no desempenho de frangos alojados em diferentes densidades (10, 16 e 22 aves/m²) e nas características da cama de aviário em galpão convencional. O experimento foi realizado durante o verão, utilizando 3328 aves da linhagem Hubbard, distribuídas em um delineamento inteiramente casualizado, em um esquema fatorial 3x2 (densidade e sexo), com quatro repetições dentro de cada ambiente. Para a comparação dos ambientes, utilizou-se a análise conjunta. Durante o período experimental, registraram-se dados de temperatura ambiente em vários pontos do galpão e em diversos horários, umidade relativa e carga térmica radiante. O teor de nitrogênio e matéria seca e a temperatura da cama foram determinados. No ambiente com isolante térmico, as temperaturas ambientais médias e a amplitude térmica diária foram menores e a umidade relativa do ar, maior. O desempenho das aves foi melhor no ambiente com isolante térmico, no qual as aves tiveram maior consumo de ração, maior ganho de peso, melhor conversão alimentar, menor mortalidade e maior produção por área. A temperatura retal das aves do ambiente sem isolante térmico foi mais elevada. À medida que se elevou a densidade, a mortalidade aumentou, porém a produção por área foi maior. O uso do isolante não influenciou a produção de cama, mas diminuiu a porcentagem de nitrogênio na cama. Com o aumento da densidade, a produção de cama elevou-se, pelo maior volume de excretas, mas não influenciou sua porcentagem de nitrogênio. O uso do isolante térmico permitiu aumento da densidade de criação dos frangos.
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Woon, Amanda P., Abolghasem Tohidpour, Hernan Alonso, Yumiko Saijo-Hamano, Terry Kwok, and Anna Roujeinikova. "Conformational Analysis of Isolated Domains of Helicobacter pylori CagA." PLoS ONE 8, no. 11 (November 1, 2013): e79367. http://dx.doi.org/10.1371/journal.pone.0079367.
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BEDEAWY SALIH, WALEED, and JASSIM MAHAMMED SALIH. "EFFECT CAMA- RAYS OF PHYSICSL PROPERTIES TO SAME ISOLATES." Journal of University of Anbar for Pure Science 3, no. 2 (August 1, 2009): 142–52. http://dx.doi.org/10.37652/juaps.2009.15311.
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Dajoh, Tessalonica, Robert A. Bara, Esther Angkouw, Medy Ompi, Rosita A. Lintang, and Cykca Lumenta. "UJI AKTIVITAS ANTIBAKTERI DAN ANTI-UV Phyllidiella nigra DAN BAKTERI SIMBIOTIKNYA DARI PERAIRAN TANJUNG MANDOLANG." JURNAL PESISIR DAN LAUT TROPIS 8, no. 2 (May 30, 2020): 61. http://dx.doi.org/10.35800/jplt.8.2.2020.29768.
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Phyllidiella nigra is an organism that is suspected to have secondary metabolites because their ability to develop its self defense system by camouflage and using chemical compounds derived from their nature diet as deterrent against their predators. The purpose of this study was to isolate symbiotic bacterial derived from P. nigra, extracted and followed by, the antibacterial assays against Escherichia coli and Bacillus megaterium as well as the anti-UV assay. The results showed that the five isolates tested had an antibacterial activity with the highest average inhibition zone against E. coli DSM 498 bacteria, isolate 1 (14.67 mm), isolate 5 (14 mm), and against B. Megaterium DSM 32T bacteria, isolate 3 (13.33 mm). The three isolates which had the highest inhibition zone and P. nigra extract were tested for anti-UV assay using a UV-Vis Spectrophotometer. The results obtained isolate 3 has absorption of UV-A with the UV absorbtion maximum at λ 340 nm and P. nigra extract has absorption on UV-B radiation with UV absorption maximum at λ 290 nm. Key words: Nudibranchia, Bacteria, Anti-bacteial, Anti-UV Phyllidiella nigra merupakan organisme yang diduga memiliki metabolit sekunder karena mampu mengembangkan sistem pertahanan dirinya dengan cara kamuflase dan menggunakan senyawa kimia sebagai racun yang didapat dari makanannya. Tujuan dari penelitian ini yaitu mendapatkan isolat bakteri yang bersimbiosis dengan P. nigra, mendapatkan ekstrak dari baktri simbion, dan menguji antibakteri dan anti-UV ekstrak etil aseta bakteri simbion dengan metode difusi agar terhadap bakteri Escherichia coli dan Bacillus megaterium. Hasil penelitian didapatkan kelima isolat yang diuji memiliki aktivitas antibakteri dengan rerata zona hambat tertinggi terhadap bakteri E. coli DSM 498 yaitu isolat 1 (14,67 mm), isolat 5 (14 mm), dan terhadap baktri B. megaterium DSM 32T yaitu isolat 3 (13,33 mm). Ketiga isolat yang memiliki zona hambat tertinggi dan ekstrak P. nigra diujikan anti-UV menggunakan alat UV-Vis Spektrofotometer. Hasil yang didapat isolat 3 memiliki serapan terhadap radiasi sinar UV-A dengan puncak tertinggi pada λ 340 nm dan ekstrak P. nigra memiliki serapan terhadap radiasi sinar UV-B dengan puncak tertinggi berada pada λ 290 nm. Kata kunci: Nudibranchia, Bacteria, Anti-bacteial, Anti-UV
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Zilli, Jerri Édson, Romano Roberto Valicheski, Norma Gouvêa Rumjanek, Jean Luis Simões-Araújo, Francisco Rodrigues Freire Filho, and Maria Cristina Prata Neves. "Eficiência simbiótica de estirpes de Bradyrhizobium isoladas de solo do Cerrado em caupi." Pesquisa Agropecuária Brasileira 41, no. 5 (May 2006): 811–18. http://dx.doi.org/10.1590/s0100-204x2006000500013.
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O objetivo deste trabalho foi avaliar as relações filogenéticas de estirpes de Bradyrhizobium e a contribuição destas estirpes para a fixação biológica de nitrogênio em caupi, em solos do Cerrado. Na avaliação da relação filogenética, o gene 16S rDNA de cada uma das estirpes foi amplificado e seqüenciado, e para a análise da eficiência simbiótica, determinou-se: N total, matéria seca das plantas, massa de nódulos e redução de acetileno, em casa de vegetação, e ocupação nodular, em experimento de campo. A maioria das estirpes estudadas pertence a B. elkanii e, pelo menos dez das estirpes, independentemente da espécie, apresentaram bom desempenho quanto à fixação biológica de N2. As estirpes BR3262, BR3280 (caracterizadas como B. elkanii) e BR3267, BR3287 e BR3288 (Bradyrhizobium sp.) mostram-se como inoculantes potenciais para o caupi, em razão do bom desempenho tanto na eficiência simbiótica quanto na ocupação nodular.
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Pereira, Elzânia Sales, Augusto César de Queiroz, Mario Fonseca Paulino, Paulo Roberto Cecon, Sebastião de Campos Valadares Filho, Lídia Ferreira Miranda, Alberto Magno Fernandes, and Luciano da Silva Cabral. "Determinação das frações protéicas e de carboidratos e taxas de degradação in vitro da cana-de-açúcar, da cama de frango e do farelo de algodão." Revista Brasileira de Zootecnia 29, no. 6 (December 2000): 1887–93. http://dx.doi.org/10.1590/s1516-35982000000600039.
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O objetivo do presente estudo foi determinar o fracionamento e a cinética de degradação dos compostos nitrogenados e dos carboidratos da cana-de-açúcar, da cama de frango e do farelo de algodão. Para determinação das frações nitrogenadas, foram analisados os compostos nitrogenados não-protéicos, nitrogênio solúvel e insolúvel em tampão borato-fosfato e nitrogênio protéico insolúvel em detergente neutro e em detergente ácido. As taxas de degradação das frações protéicas foram obtidas in vitro, a partir da incubação dos alimentos com proteases isoladas do meio ruminal. Para obtenção do fracionamento dos carboidratos, de acordo com o sistema Cornell, foram calculados os carboidratos totais, as suas frações C, B2 e os componentes solúveis em detergente neutro. Foram determinadas as frações potencialmente degradável (B2) e indegradável (C) da fibra em detergente neutro, corrigida para cinzas e proteína, e as taxas de latência dinâmica, de degradação e de crescimento específico dos microrganismos, a partir da fração B2, além dos coeficientes de degradabilidade e do efeito de repleção ruminal da FDN dos alimentos. Valores de 71,01; 45,80; e 71,66% da fração B2 dos compostos nitrogenados foram encontrados para cana-de-açúcar, cama de frango e farelo de algodão, respectivamente. A cama de frango destacou-se pela maior fração B3 e C (23,57 e 10,11%). As taxas de degradação da fração B3 foram 0,03; 0,031; e 0,09 h-1 para cana-de-açúcar, cama de frango e farelo de algodão, respectivamente. A fração C dos carboidratos foi 22,74; 28,00; e 32,64% para os três alimentos, respectivamente. A cana-de-açúcar apresentou elevada fração de componentes solúveis (35,99%), porém mostrou baixa digestão da fração potencialmente degradável (3,41% h-1), a qual apresentou significativo efeito de repleção (6,6 h-1). Com estas técnicas, foi possível detectar que alimentos como cana-de-açúcar e cama de frango apresentam baixo valor nutricional para atendimento das exigências dos microrganismos do rúmen e hospedeiro.
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Istifadah, Noor, Putu Ghita Novilaressa, Fitri Widiantini, and Sri Hartati. "Keefektifan Bakteri dan Khamir Asal Air Rendaman Kompos dalam Menekan Perkembangan Penyakit Bercak Coklat (Alternaria solani Sorr.) pada Tomat." Agrikultura 31, no. 1 (May 15, 2020): 52. http://dx.doi.org/10.24198/agrikultura.v31i1.26876.
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Penyakit bercak coklat yang disebabkan oleh jamur Alternaria solani Sorr. merupakan salah satu penyakit penting pada tanaman tomat. Cara pengendalian penyakit bercak coklat yang biasa dilakukan adalah dengan penyemprotan fungisida sintetik. Mengingat berbagai dampak negatif dari penggunaan pestisida yang terus-menerus, maka perlu dikembangakan cara pengendalian ramah lingkungan seperti pengendalian secara biologi. Bakteri dan jamur merupakan mikrob yang berpotensi sebagai agens biokontrol penyakit tanaman. Salah satu sumber dari agens antagonis patogen tanaman adalah air rendaman kompos. Paper ini mendiskusikan hasil penelitian yang mengevaluasi kemampuan bakteri mikrob yang diisolasi dari air rendaman kompos berbahan dasar kotoran sapi dan domba untuk menghambat pertumbuhan A. solani in vitro dan menekan penyakit yang disebabkan patogen tersebut pada buah dan tanaman tomat. Percobaan secara in vitro menggunakan Rancangan Acak Lengkap, sementara pengujian pada buah dan tanaman tomat menggunakan Rancangan Acak Kelompok. Isolasi mikrob dari air rendaman kompos berbahan dasar kotoran sapi dan domba menghasilkan 35 isolat, yang mana 11 isolat (enam isolat bakteri dan lima isolat khamir) dapat menghambat pertumbuhan A. solani secara in vitro sebesar 79,3%-84,2% dengan zona hambat sebesar 0,0-28,3 mm. Pada pengujian secara in vivo, lima isolat non-patogenik (dua isolat bakteri dan tiga isolat khamir) dapat menekan penyakit bercak coklat pada buah tomat sebesar 100% dan pada daun tomat sebesar 77,5%-98,1%. Isolat-isolat ini berpotensi untuk dikembangkan lebih lanjut sebagai agens biokontrol penyakit bercak coklat pada tanaman tomat.
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Agustinur, Agustinur, and Yusrizal Yusrizal. "EKSPLORASI JAMUR ASAL TONGKOL KOSONG KELAPA SAWIT YANG BERPOTENSI SEBAGAI AGEN PENDEGRADASI SELULOSA." Jurnal Agrotek Tropika 9, no. 3 (September 30, 2021): 533. http://dx.doi.org/10.23960/jat.v9i3.5128.
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Salah satu permasalahan yang dihadapi dalam bidang pertanian adalah melimpahnya limbah yang belum dapat dimanfaatkan secara optimal seperti tandan kosong kelapa sawit (TKKS). Komponen terbesar yang terkandung di dalam TKKS adalah selulosa. Komponen ini cenderung sulit terdegradasi di alam. Beberapa agens hayati dari kelompok jamur diketahui dapat mendegradasi selulosa karena memiliki kemampuan selulolitik. Penelitian ini bertujuan untuk mengisolasi jamur asal limbah TKKS yang memiliki potensi untuk mendegradasi selulosa, sehingga nantinya dapat dimanfaatkan dalam mendegradasi limbah TKKS secara optimal. Tahapan penelitian dimulai dari pengambilan sampel TKKS terdekomposisi yang berada di area perkebunan kelapa sawit PT Agro Sinergi Nusantara Kabupaten Aceh Jaya, Aceh. Kemudian dari sampel tersebut dilakukan tahapan isolasi jamur. Hasil isolasi tersebut telah diperoleh sebanyak 18 isolat jamur. Selanjutnya isolat-isolat tersebut dipurifikasi dan diuji potensi selulolitiknya dengan cara ditumbuhkan pada media CMC. Berdasarkan hasil uji potensi selulolitik diperoleh sebanyak 17 isolat yang memiliki potensi dengan indikator terbentuknya zona bening di sekeliling koloni. Di antara 17 isolat tersebut terdapat 3 isolat bakteri dengan kategori potensi selulolitik tinggi yaitu isolat JM714, JM402 dan JM609, dengan indeks selulolitik berturut-turut sebesar 6,5, 3,47 dan 2,1. Setelah dilakukan identifikasi isolat JM714 diduga merupakan anggota genus Aspergillus, sementara isolat JM402 dan JM609 adalah anggota genus Trichoderma.
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Fabry, Cleia F. S., Leandro G. Freitas, Wânia S. Neves, Marcelo M. Coutinho, Marcos R. Tótola, José R. Oliveira, Rosangela Dallemole-Giaretta, and Silamar Ferraz. "Obtenção de bactérias para a o biocontrole de Meloidogyne javanica por meio de aquecimento de solo e tratamento com filtrado de raízes de plantas antagonistas a fitonematóides." Fitopatologia Brasileira 32, no. 1 (February 2007): 79–82. http://dx.doi.org/10.1590/s0100-41582007000100013.
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Cinco amostras de solo com alto teor de matéria orgânica (10,14 dag/kg) de 1 kg cada foram autoclavadas (120 °C/1h), cinco foram aquecidas em forno de microondas a 660 watts e 2450 Hz por 4 min e cinco não foram aquecidas. Raízes de Mucuna aterrima, Crotalaria juncea, Tagetes erecta e Lycopersicon esculentum foram maceradas separadamente em liquidificador à baixa velocidade durante 30 s em 1000 mL de água e peneiradas. As suspensões resultantes foram adicionadas às amostras de solos as quais foram acondicionadas em saco plástico e submetidas aos três tratamentos térmicos e posteriormente mantidas a 28 ºC por 24 h. Setenta e oito isolados bacterianos foram obtidos das amostras de solo por diluição em série e submetidos à seleção para o biocontrole de M. javanica. Sementes de tomate foram microbiolizadas por imersão em suspensão de propágulos de cada uma das culturas e semeadas em substrato dentro de tubetes em casa de vegetação. As mudas resultantes foram inoculadas com 400 ovos do nematóide, cada. O isolado UFV-6 de Escherichia coli reduziu o número de galhas em maior magnitude (80%) ao passo que o isolado UFV-8 de Citrobacter freundii foi o mais eficiente na redução do número de ovos (83%). A identificação dos isolados foi feita por análise de ácidos graxos esteres de metil e testes bioquímicos.
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Karaman, Kamuran, Hatice Tuba Akbayram, Sinan Akbayram, Mesut Garipardıç, and Ahmet Fayik Öner. "İzole Lenfadenopatiyle Başvuran Atipik Bir Bruselloz Olgusu." Journal of Pediatric Infection 11, no. 1 (March 30, 2017): 43–45. http://dx.doi.org/10.5578/ced.61871.
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Burton, Ralph R., A. Gadian, A. M. Blyth, and S. D. Mobbs. "Modelling isolated deep convection: A case study from COPS." Meteorologische Zeitschrift 22, no. 4 (August 1, 2013): 433–43. http://dx.doi.org/10.1127/0941-2948/2013/0408.
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Arakeri, Dr Satish, Dr Uroos Fatima, and Dr Ramkumar KR Dr.Ramkumar KR. "Isolated Carpal and Metacarpal Bone Tuberculosis - A Case Report." Paripex - Indian Journal Of Research 3, no. 3 (January 15, 2012): 130–32. http://dx.doi.org/10.15373/22501991/mar2014/46.
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K, Prabhakar, Jayarama N, Pavithra L, and Anil Kumar M. "Rare Case of Snake Bite With Severe Isolated Hematotoxicity." JOURNAL OF CLINICAL AND BIOMEDICAL SCIENCES 01, no. 4 (December 15, 2011): 173–77. http://dx.doi.org/10.58739/jcbs/v01i4.1.
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Novitasari, Alifia Rizky, Woro Hastuti Satyantini, Sapto Andriyono, and Nor Sa'adah. "Isolasi dan Identifikasi Bakteri Pengurai Mikroplastik Polyethylene Terephthalate dari Sedimen Ekosistem Mangrove Pasir Putih." Journal of Marine Research 12, no. 1 (January 31, 2023): 52–60. http://dx.doi.org/10.14710/jmr.v12i1.37503.
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Polyethylene terephthalate (PET) adalah salah satu polimer termoplastik paling banyak tersedia di pasar. PET digunakan untuk produksi botol atau wadah untuk minuman, seperti air, minuman ringan berkarbonasi, minuman jus, dan industri makanan. Polyethylene terephthalate (PET) ditemukan melimpah pada sedimen ekosistem mangrove di Pasir Putih Wonorejo pada musim hujan sebanyak 59%. Studi terbaru telah menunjukkan bahwa bakteri indigenous sedimen mangrove mampu mendegradasi mikroplastik. Sehingga, tujuan penelitian ini adalah menemukan potensi agen biodegradasi mikroplastik dari bakteri indigenous sedimen ekosistem mangrove di Pasir Putih Wonorejo. Bakteri diisolasi pada April 2022 dari sedimen ekosistem mangrove. Metode yang digunakan diawali dengan isolasi bakteri dari sedimen ekosistem mangrove Pasir Putih Wonorejo, kemudian digunakan untuk uji degradasi mikroplastik polyethylene terephthalate dan mengetahui jenis bakteri yang memiliki kemampuan mendegradasi mikroplastik. Hasil isolat bakteri yang didapat sebanyak 16 bakteri. Potensi bakteri yang menjadi agen biodegradasi dengan penurunan berat mikroplastik isolat bakteri SPP.1.1.3. sebesar 8,73%. Identifikasi molekuler dengan cara ekstraksi DNA 16S rRNA menunjukkan jenis Pseudoaltoromonas caenipelagi dengan homologi 99% pada database National Center Biotechnology Information. Hasil ini membuktikan bahwa bakteri indigenous memiliki kemampuan sebagai agen biodegradasi mikroplastik. Polyethylene terephthalate (PET) is one of the most widely available thermoplastic polymers on the market. PET is used for the production of bottles or containers for beverages, such as water, carbonated soft drinks, juices, and the food industry. Polyethylene terephthalate (PET) is found abundantly in mangrove ecosystem sediments in Pasir Putih Wonorejo during the rainy season as much as 59%. Recent studies have shown that indigenous mangrove sediment bacteria are able to degrade microplastics. Thus, the purpose of this study was to find potential agents for biodegradation of microplastics from indigenous bacteria in mangrove ecosystem sediments Pasir Putih Wonorejo. Bacteria were isolated in April 2022 from mangrove ecosystem sediments. The method used begins with The isolation of bacteria from mangrove ecosystems sediments from Pasir Putih Wonorejo, then were used to assay biodegradation of polyethylene terephthalate microplastics and to determine the types of bacteria that has ability to degrade microplastics. The results of isolated bacterial obtained were 16 bacterias. The potential bacteria that becoming agents of biodegradation by decreasing the weight of microplastic isolate SPP.1.1.3. by 8,73%. Molecular identification by extraction of DNA 16S rRNA showed the type of Pseudoaltoromonas caenipelagi with 99% homology in the National Center Biotechnology Information database. These results prove that indigenous bacteria have the ability as microplastic biodegradation agents.
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Ramos, Nilza Patrícia, Maria do Carmo de Salvo Soares Novo, Antônio Augusto Lago, and Guilherme Calderari Marin. "Emergência de plântulas e crescimento inicial de cultivares de amendoim sob resíduos de cana-de-açúcar." Revista Brasileira de Sementes 30, no. 1 (2008): 190–97. http://dx.doi.org/10.1590/s0101-31222008000100024.
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O objetivo do trabalho foi avaliar o efeito da interação palha de cana-de-açúcar e aplicação de vinhaça na emergência de plântulas e no crescimento inicial de cultivares de amendoim. O experimento foi realizado em vasos dispostos em blocos ao acaso, com quatro repetições, sendo os tratamentos arranjados fatorialmente (5 x 2 x 3) e consistindo da combinação de cinco quantidades de palha (0, 5, 10, 15 e 20 t ha-1) e a aplicação ou não de 150 m³ ha-1 de vinhaça nas cultivares de amendoim IAC-Caiapó, Runner 886 e Tatu. Determinou-se a velocidade e a porcentagem final de emergência de plântulas, além de altura e biomassa seca da parte aérea de plantas. Conclui-se que, nas condições de casa-de-vegetação, a presença da palha de cana-de-açúcar e da vinhaça, em quantidade equivalente a 150 m³ ha-1, em ação conjunta ou isolada, prejudicam a emergência e o crescimento de plântulas de amendoim, sendo que a cultivar Tatu é a mais tolerante em relação as cultivares ‘Runner 886’ e ‘IAC-Caiapó’.