Dissertations / Theses on the topic 'Carico organico'

L’attuale condizione che caratterizza il settore energetico richiede un necessario processo di riconversione che, oltre a favorire il risparmio energetico, riduca la dipendenza dai combustibili fossili ed accresca l’impiego di fonti energetiche rinnovabili, dando un contributo fondamentale alla riduzione delle emissioni di gas serra come diversi accordi internazionali richiedono. Si rende pertanto necessario accelerare i processi che da alcuni anni stanno favorendo l’utilizzo di energia da fonti rinnovabili. Tra queste, le fonti legate ai processi di trattamento biologico dei reflui stanno avendo un interessante sviluppo. Esistono numerosi processi biologici che consentono la produzione di energia in maniera indiretta, quali ad esempio i processi di digestione anaerobica finalizzati alla produzione di biogas e/o produzione biologica di idrogeno. In tale contesto si inserisce la tecnologia delle Microbial Fuel Cell, che consente la produzione diretta di energia elettrica, finalizzata al recupero energetico inteso al miglioramento dell’efficienza energetica e alla riduzione dei costi d’esercizio di impianti di trattamento biologico dei reflui. Il presente lavoro di Tesi di Dottorato sperimentale, svoltosi in collaborazione al laboratorio PROT.-IDR. della sede ENEA di Bologna, riporta i risultati dell’attività di ricerca condotta su una MFC (Microbial Fuel Cell) a doppio stadio biologico per il trattamento di reflui ad elevato carico organico e produzione continua di energia elettrica. E’ stata provata l’applicabilità della MFC con entrambi i comparti biotici utilizzando elettrodi di grafite non trattata ottenendo, con un carico organico in ingresso di circa 9 gd-1, valori di potenza massima prodotta che si attestano su 74 mWm-2, corrente elettrica massima generata di 175 mAm-2 ad una tensione di 421 mV, ed una conversione di COD in elettricità pari a 1,2 gCODm-2d-1. I risultati sono stati molto positivi per quanto riguarda le prestazioni depurative ottenute dalla MFC. L’efficienza di depurazione misurata ha raggiunto un valore massimo del 98% di rimozione del COD in ingresso, mentre e la concentrazione di azoto ammoniacale nell’effluente raccolto all’uscita del sedimentatore è sempre stata inferiore a 1 mgN-NH4+l-1. Tra gli obiettivi posti all’inizio della sperimentazione si è rivelata di notevole interesse la valutazione del possibile utilizzo della MFC come sistema per il monitoraggio on-line del COD e degli acidi grassi volatili (VFA) prodotti all’interno di un digestore anaerobico, attraverso la definizione di una correlazione tra i dati elettrici registrati in continuo e le concentrazioni di CODanaer e VFA misurate in diversi periodi della sperimentazione. L’analisi DGGE della biomassa catodica ha fornito uno strumento analitico utile allo studio della diversità della comunità microbica sospesa ed adesa al catodo e ha confermato la forte similarità delle specie batteriche riconosciute nei campioni analizzati. In particolare, le bande di sequenziamento ottenute sono affiliate ai gruppi batterici Firmicutes, -Proteobacteria,  -Proteobacteria, -Proteobacteria e Bacteroidetes. Da quanto emerso dalla sperimentazione condotta si può pertanto concludere che ad oggi le MFC sono in fase di evoluzione rispetto ai primi prototipi utilizzati per lo studio delle comunità microbiali e per la comprensione dei meccanismi di trasferimento elettronico. Sfruttarne la potenza prodotta in maniera commerciale diviene una grande sfida per il futuro, ed è opinione comune che le prime applicazioni pratiche delle MFC saranno come fonte di recupero energetico per i dispositivi utilizzati per il monitoraggio dell’ambiente e per il trattamento delle acque reflue.

L’attuale condizione che caratterizza il settore energetico richiede un necessario processo di riconversione che, oltre a favorire il risparmio energetico, riduca la dipendenza dai combustibili fossili ed accresca l’impiego di fonti energetiche rinnovabili, dando un contributo fondamentale alla riduzione delle emissioni di gas serra come diversi accordi internazionali richiedono. Si rende pertanto necessario accelerare i processi che da alcuni anni stanno favorendo l’utilizzo di energia da fonti rinnovabili. Tra queste, le fonti legate ai processi di trattamento biologico dei reflui stanno avendo un interessante sviluppo. Esistono numerosi processi biologici che consentono la produzione di energia in maniera indiretta, quali ad esempio i processi di digestione anaerobica finalizzati alla produzione di biogas e/o produzione biologica di idrogeno. In tale contesto si inserisce la tecnologia delle Microbial Fuel Cell, che consente la produzione diretta di energia elettrica, finalizzata al recupero energetico inteso al miglioramento dell’efficienza energetica e alla riduzione dei costi d’esercizio di impianti di trattamento biologico dei reflui. Il presente lavoro di Tesi di Dottorato sperimentale, svoltosi in collaborazione al laboratorio PROT.-IDR. della sede ENEA di Bologna, riporta i risultati dell’attività di ricerca condotta su una MFC (Microbial Fuel Cell) a doppio stadio biologico per il trattamento di reflui ad elevato carico organico e produzione continua di energia elettrica. E’ stata provata l’applicabilità della MFC con entrambi i comparti biotici utilizzando elettrodi di grafite non trattata ottenendo, con un carico organico in ingresso di circa 9 gd-1, valori di potenza massima prodotta che si attestano su 74 mWm-2, corrente elettrica massima generata di 175 mAm-2 ad una tensione di 421 mV, ed una conversione di COD in elettricità pari a 1,2 gCODm-2d-1. I risultati sono stati molto positivi per quanto riguarda le prestazioni depurative ottenute dalla MFC. L’efficienza di depurazione misurata ha raggiunto un valore massimo del 98% di rimozione del COD in ingresso, mentre e la concentrazione di azoto ammoniacale nell’effluente raccolto all’uscita del sedimentatore è sempre stata inferiore a 1 mgN-NH4+l-1. Tra gli obiettivi posti all’inizio della sperimentazione si è rivelata di notevole interesse la valutazione del possibile utilizzo della MFC come sistema per il monitoraggio on-line del COD e degli acidi grassi volatili (VFA) prodotti all’interno di un digestore anaerobico, attraverso la definizione di una correlazione tra i dati elettrici registrati in continuo e le concentrazioni di CODanaer e VFA misurate in diversi periodi della sperimentazione. L’analisi DGGE della biomassa catodica ha fornito uno strumento analitico utile allo studio della diversità della comunità microbica sospesa ed adesa al catodo e ha confermato la forte similarità delle specie batteriche riconosciute nei campioni analizzati. In particolare, le bande di sequenziamento ottenute sono affiliate ai gruppi batterici Firmicutes, -Proteobacteria,  -Proteobacteria, -Proteobacteria e Bacteroidetes. Da quanto emerso dalla sperimentazione condotta si può pertanto concludere che ad oggi le MFC sono in fase di evoluzione rispetto ai primi prototipi utilizzati per lo studio delle comunità microbiali e per la comprensione dei meccanismi di trasferimento elettronico. Sfruttarne la potenza prodotta in maniera commerciale diviene una grande sfida per il futuro, ed è opinione comune che le prime applicazioni pratiche delle MFC saranno come fonte di recupero energetico per i dispositivi utilizzati per il monitoraggio dell’ambiente e per il trattamento delle acque reflue.

Questo lavoro è incentrato sull'analisi e la simulazione di un ciclo Rankine a fluido organico (ORC) per la produzione di energia da calore a bassa entalpia. Il lavoro è stato svolto in collaborazione con l'università di Gent, in Belgio, in particolare grazie al Prof. De Peape e all' Ing. Steven Lecompte; il lavoro in Italia è stato coordinato dal Prof. De Pascale. L'obiettivo principale della tesi è stata la creazione un modello computazionale in grado di descrivere le prestazioni dell'impianto a carico parziale. Ogni elemento dell'impianto è stato analizzato e modellizzato secondo un approccio di simulazione originale in Matlab. I componenti ottenuti sono stati poi combinati a formare la simulazione dell'intero sistema; questa è in grado di prevedere la potenza prodotta ed il rendimento del ciclo al variare delle condizioni esterne. I risultati ottenuti dalle simulazioni sono stati poi confrontati con i dati sperimentali raccolti sull'impianto di prova dell'Università. Questo confronto dimostra un buon accordo dei risultati previsti con quelli misurati. L'errore relativo massimo ottenuto per la potenza elettrica è sotto il 7%, così come quello sul rendimento; Gli errori relativi a pressione ed entalpie sono in genere sotto il 5%. Il modello può perciò dirsi validato e rappresenta un valido strumento di analisi per comprendere la risposta ai carichi parziali anche al di fuori del range di calibrazione. Il modello è stato infine utilizzato per costruire delle mappe del carico parziale e analizzare l'influenza delle variabili del ciclo. Ulteriori applicazioni delle mappe prodotte sono proposte a fine del lavoro.

Uno dei settori che più si stanno sviluppando nell'ambito della ricerca applicata è senza dubbio quello dell'elettronica organica. Nello specifico lo studio è sospinto dagli indubbi vantaggi che questi dispositivi porterebbero se venissero prodotti su larga scala: basso costo, semplicità realizzativa, leggerezza, flessibilità ed estensione. È da sottolineare che dispositivi basati su materiali organici sono già stati realizzati: si parla di OLED (Organic Light Emitting Diode) LED realizzati sfruttando le proprietà di elettroluminescenza di alcuni materiali organici, OFET (Organig Field Effect Transistor) transistor costruiti con semiconduttori organici, financo celle solari che sfruttano le buone proprietà ottiche di questi composti. Oggetto di analisi di questa tesi è lo studio delle proprietà di trasporto di alcuni cristalli organici, al fine di estrapolarne la mobilità intrinseca e verificare come essa cambi se sottoposti a radiazione x. I due cristalli su cui si è focalizzata questa trattazione sono il 1,5-Dinitronaphtalene e il 2,4-Dinitronaphtol; su di essi è stata eseguita una caratterizzazione ottica e una elettrica, in seguito interpretate con il modello SCLC (Space Charge Limited Current). I risultati ottenuti mostrano che c'è una differenza apprezzabile nella mobilità nei due casi con e senza irraggiamento con raggi x.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-05-30T13:53:16Z No. of bitstreams: 1 2015 - Thiago Cunha Silverio.pdf: 923183 bytes, checksum: 214eb765e4eeb06060ac4c9f90c393e3 (MD5)
Made available in DSpace on 2017-05-30T13:53:16Z (GMT). No. of bitstreams: 1 2015 - Thiago Cunha Silverio.pdf: 923183 bytes, checksum: 214eb765e4eeb06060ac4c9f90c393e3 (MD5) Previous issue date: 2015-12-04
Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq
The study was conducted, in the center of Integrated Rural Education State-CEIER, located in the municipality of White Eagle, Northwest of the State of Esp?rito Santo, the aim of this study was to evaluate the action of the whole milk added to the Bordeaux mixture Asperisporium caricae control, causative agent of smallpox and the behavior of this disease in different population densities the papaya tree. In setting up the experiment, the seedlings were separated by size: smaller, medium-sized and larger, so that the implementation of the orchard, there was within each block seedlings with similar vigor. The seedlings of papaya (Carica papaya L.) were prepared, using seeds of papayas hermaphrodites of the cultivar ' Golden Sunrise ', belonging to the soil. On planting and leading between each block, was grown legumes Sunn hemp (Crotalaria juncea) as green manure. The plants were bi-monthly composted manure tanned and potassium sulphate and alternating sprays with foliar fertilizer Agrobio (2%). The phytosanitary control followed the rules of organic farming. Spontaneous herbs were controlled by means of plant cover (Sunn hemp biomass) and periodic ro?adas. It took the experimental design of randomized blocks, being arranged in factorial arrangement of treatments. We evaluated the whole milk concentrations (0,0%, 5% and 10%) + 1% Bordeaux mixture (subplots), in six different breeding densities (1667, 2167, 3167, 2667, 3667 and 4167 plants. hectare-1). Based on the results it is concluded that: the addition of raw cow's milk to the Bordeaux mixture enables greater control of smallpox of papaya, both on the leaves, as in the fruits; the mixture prepared with 10% of raw cow's milk in Bordeaux mixture, the greater the inhibition signs of smallpox from papaya, both on the leaves, as in the fruits; analyzing by vegetative aspect of papaya, the greatest effect of the addition of raw cow's milk in Bordeaux mixture occurs in younger leaves; the extent to which increasing the dosage raw cow's milk, from zero to 10%, in Bordeaux, increases linearly, the ability to control the smallpox of the papaya, both on the leaves, as in the fruits; less degradated plantations allow lower occurrence of smallpox from papaya, both on the leaves, as in the fruits, if compared to those more Caterpillar; the density of plantation of papaya of 1667 plants.ha-4167 plants even 1.ha-1 the severity of smallpox of the papaya tree increases in a linear fashion; smallpox papaya progresses, exponentially, from bottom to top and/or older leaves to the newest; the part of the fruit, stuck in the plant, yet more exposed to external factors, is the most affected by smallpox of papaya, compared with the part facing the stem; analyzing for the papaya reproductive aspect, the greatest effect of the addition of raw cow's milk in Bordeaux is noticed on the face less exposed of the fruit; and, eat avocado with more leaves infected by smallpox will produce fruits also more affected by this disease
O estudo foi conduzido, no Centro Estadual Integrado de Educa??o Rural ? CEIER, situado no munic?pio de ?guia Branca, Regi?o Noroeste do Estado do Esp?rito Santo, o objetivo deste trabalho foi avaliar a a??o do leite integral adicionado ? calda bordalesa no controle do Asperisporium caricae, agente causal da var?ola e o comportamento desta doen?a em diferentes densidades de cultivo do mamoeiro. Na montagem do experimento, as mudas foram separadas por tamanho: menores, m?dias e maiores, de forma que na implanta??o do pomar, houvesse dentro de cada bloco mudas com padr?o de vigor parecido. As mudas de mamoeiro (Carica papaya L.) foram preparadas, utilizando-se sementes de mam?es hermafroditas da cultivar ?Sunrise Golden?, pertencente ao grupo Solo. Na entrelinha de plantio e entre cada bloco, foi cultivada a leguminosa crotal?ria (Crotalaria juncea) como adubo verde. As plantas foram bimensalmente adubadas com esterco curtido e sulfato de pot?ssio e pulveriza??es alternadas, com o biofertilizante l?quido Agrobio (2%). O controle fitossanit?rio seguiu as normas da agricultura org?nica. As ervas espont?neas foram controladas por meio de cobertura vegetal (biomassa da crotal?ria) e de ro?adas peri?dicas. Adotou-se o delineamento experimental de blocos ao acaso, sendo os tratamentos dispostos em parcelas subdivididas. Avaliaram-se as concentra??es de leite integral (0,0%, 5% e 10%) + calda bordalesa 1% (subparcelas), dentro de seis densidades de cultivo diferentes (1667, 2167, 2667, 3167, 3667 e 4167 plantas.hectare-1). Com base nos resultados conclui-se que: a adi??o de leite de vaca cru ? calda bordalesa possibilita maior capacidade de controle da var?ola do mamoeiro, tanto nas folhas, quanto nos frutos; a mistura preparada com 10% de leite de vaca cru na calda bordalesa, propiciou a maior inibi??o dos sinais da var?ola do mamoeiro, tanto nas folhas, quanto nos frutos; analisando pelo aspecto vegetativo do mamoeiro, o maior efeito da adi??o do leite de vaca cru na calda bordalesa ocorre nas folhas mais novas; a medida em que se aumenta a dosagem de leite de vaca cru, de zero at? 10%, na calda bordalesa, aumenta-se, linearmente, a capacidade de controle da var?ola do mamoeiro, tanto nas folhas, quanto nos frutos; plantios menos adensados possibilitam menor ocorr?ncia da var?ola do mamoeiro, tanto nas folhas, quanto nos frutos, se comparados ?queles mais adensados; da densidade de plantio do mamoeiro de 1667 plantas.ha-1 at? 4167 plantas.ha-1 a severidade da var?ola do mamoeiro aumenta de maneira linear; a var?ola do mamoeiro progride, exponencialmente, de baixo para cima e/ou das folhas mais velhas para as mais novas; a parte do fruto, preso ainda na planta, mais exposta aos fatores externos, ? a mais acometida pela var?ola do mamoeiro, se comparado com a parte voltada para o caule da mesma; analisando pelo aspecto reprodutivo do mamoeiro, o maior efeito da adi??o do leite de vaca cru na calda bordalesa ? notado na face menos exposta do fruto; e, mamoeiros com as folhas mais infectadas pela var?ola produzir?o frutos tamb?m mais acometidos por esta doen?a.

Made available in DSpace on 2016-12-08T16:44:47Z (GMT). No. of bitstreams: 1 PGPV14MA142.pdf: 646523 bytes, checksum: e0bd829f134f5d24d758fee703f47aa9 (MD5) Previous issue date: 2014-06-27
The objectives of the survey and typing traditional orchards subsistence containing fig plants in rural and urban perimeter Catarina were performing. Characterize the roughness of the fig tree clones and evaluate the use of homeopathic preparations in fig rust control in organic production systems. The metodology used for the survey and location of fig germplasm nigger were held through technical visits. We conducted 43 interviews with semi-structured questionnaire, rising information about the history, potential use, ways to use the fruit and procedures for management of fig. For characterization of resistance expedriments were conducted at the Experimental Station of the Agriocultural Research and Rural Extension, Lages, SC and the Federal Institute of Santa Catarina Concord Campus, in the period 2013/2014. The experiments consisted of evaluation of 35 clones in four replication, with and experimental ploto f fours plants, the revews were incidence and severity of rust on the leaves fortnightly. The study wth the use of homeopathic preparations was conducted in the greenhouse and the field at trhe Federal Institute of Santa Catarina Concord Campus, SC, in two cropping cycles, 2012/2014. The experimental design was a randomized block with four replications, the field and eight replicates in a greenhouse. The homeopatic treatments were prepared: Belladonna, Ferrum fosforicum, Aconitum napelus and biotherapic of fig leaves, all in CH12 (twelfth order of Hahnemann s centesimal dolution), Bordeaux mixture 0.2% and 0.3% sulfue syruo. Witnesses were water and non-intervention. It was found that the cultivation of the fig tree is associated with the lemon, orange, bergamoteiras, vines, peac, persimmon and plum trees in backyards, near the home. Phytosanitary problems were repprted by 86% of respondentes in rural areas and 14% in the urban áreas. All respondentes (43) figs intended for personal use and also sells only one. The age of the plants of figs were between 8-65 years. Two accessions of purple figs and two accessions of fine purple fig showed less incidence and severity ofrust on the leaves. The availability of Creoles genotypes resistance to rust offers potential for use on a comercial scale in the locations studies. In the greenhouse, fig treated with Belladonna 12CH, 0.2% Bordeaux misture and lime súlfur to 0.3% showed less senescence and showed the lowest área under the disease progress curve. The field, fig treated with Belladonna 12CH, 0.2% Bordeaux mixture and lime súlfur to 0.3% also showed the lowest área under the disease progress curve and a lower incidence of rust average of seven ecaluations. Holders of knowledge about the cultivation and use of fig fruits are prevalente in the elderly. There Creoles genptypes high resistance rust. Belladonna and nosode rust were equivalente to Bordeaux mixture in most trials in the management of rust fig
Este estudo teve objetivo de reconhecer germoplasma crioulo de figo, o conhecimento associado e avaliar a resistência da figueira e uso de preparados homeopáticos no manejo da ferrugem. A localização e levantamento de germoplasma de figo crioulo foram realizadas através de visitas técnicas a área rural no Oeste Catarinense e urbana de Lages. Realizou-se 43 entrevistas com questionário semi-estruturado, levantandose informações sobre o histórico, potencial de uso, formas de utilização do fruto e procedimentos de manejo de figueiras. Para caracterização de resistência experimentos foram realizados na Estação Experimental da Empresa de Pesquisa Agropecuária e Extensão Rural, Lages, SC e no Instituto Federal Catarinense Campus Concórdia, no período de 2013/2014. Os experimentos constaram de avaliação de 35 clones em quatro repetições, sendo a parcela experimental de quatro plantas. As avaliações foram quinzenais de incidência e severidade de ferrugem nas folhas. O estudo com uso de preparados homeopáticos foi realizado em casa de vegetação e a campo no Instituto Federal Catarinense Campus Concórdia, SC, em dois ciclos de cultivo de 2012 a 2014. O delineamento experimental foi de blocos ao acaso com quatro repetições, a campo, e oito repetições, em casa de vegetação. Os tratamentos foram os preparados homeopáticos: Belladonna, Ferrum fosforicum, Aconitum napelus, e bioterápico de folhas de figueiras, todas na CH12 (décima segunda ordem de diluição centesimal hahnemanniana). Calda bordalesa a 0,2% e calda sulfocalcica a 0,3% foram os tratamentos padrão. Testemunhas foram a água e não intervenção. Verificou-se que o cultivo da figueira está associado, a limoeiros, laranjeiras, bergamoteiras, videiras, pessegueiros, caquizeiros e ameixeiras em quintais, próximos às residências. Problemas fitossanitários foram reportados por 86% dos entrevistados na área rural e 14% no perímetro urbano. Todos os entrevistados (43) destinam os figos para consumo próprio e apenas um também comercializa. A idade das plantas de figos foi entre 8 a 65 anos. Dois acessos de figo roxo e dois acessos de figo roxo fino mostraram menos incidência e severidade de ferrugem nas folhas. A disponibilidade de genótipos crioulos resistentes a ferrugem oferece potencial de uso em escala comercial nos locais estudados. Em casa de vegetação, figueiras tratadas com Belladonna 12CH, calda bordalesa a 0,2% e calda sulfocálcica a 0,3% apresentaram menor senescência e apresentaram menor índice de área abaixo da curva de progresso da doença. A campo, figueiras tratadas com Belladonna 12CH, calda bordalesa a 0,2% e calda sulfocálcica a 0,3% também apresentaram menor índice de área abaixo da curva de progresso da doença e menor incidência da ferrugem, média das sete avaliações. Detentores do conhecimento sobre o cultivo e uso de frutos de figo são predominantes de terceira idade. Belladonna e Nosódio da ferrugem foram equivalentes a calda bordalesa na maioria dos ensaios no manejo da ferrugem da figueira

Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico
This study aimed to identify cultivation techniques with biofertilizer in greenhouse and open field with fig culture in Limoeiro do Norte-CE, including three production cycles, from October 2010 to December 2011. The experimental design was a randomized block with split plot with four replications. Treatments included a combination of 3 cultivation environments: greenhouse (EST), trellis (LT) and open field (CA), which constituted the plots with 4 treatments in sub plots, consisting of 4 concentrations (biofertilizer: water) in the following way : T0 (0% biofertilizer: 100% water); T1 (20% biofertilizer: 80% water), T2 (40% biofertilizer: 60% water), T3 (60% biofertilizer: 40% water), in which there were applied 3,0 L of biofertilizer per plant every 15 days. Data were submitted to analysis of variance and when significant by F test, average tests were conducted by the tukey test at 5% probability and those concerning to biofertilizer levels were submitted to regression analysis. The amount of N in the leaf dry matter increased 19%, 21% and 11%, with the growth in biofertilizer doses, for three cycles, respectively. As for P and K, increments were 25.0%, 7.0% and 13% and 12.6%, 10.5% and 29.4%, respectively, following the cycles. In the first cycle there was no significant difference between the average yield of 10.44 and 10.14 t ha-1 observed in the LT and in the EST, respectively, but they were statistically higher to that one obtained in CA with 9.62 t ha-1. In the second cycle environment did not affect significantly the variable productivity. In the third cycle the LT showed fruit yield of 6.47 t ha-1, significantly higher than those detected in EST and in CA with 4.37 and 3.81 t h-1, respectively. As for the total yield there was no significant difference between the averages 18.83 and 18.27 t ha-1, obtained in the EST and in the CA, respectively, however, they were statistically lower than that one obtained in the TL with 22.49 t ha-1. Based on the analysis of the three cycles, it can be stated as a general conclusion, that there is viability of fig organic cultivation with biofertilizer in protected low-cost type trellis, in the semiarid. And as specific conclusions, it can be stated that: the maximum temperature in the trellis environment is up to 3.1 oC lower than the one in the greenhouse, but up to 1.7 oC higher than that one observed in the open field; the relative humidity in the open atmosphere is up to 4.1% higher than the one in the greenhouse, and up to 3.7% higher than the one in the trellis. Water consumption in the trellis represented 87.3% of the registered in open field, but it was 16.4% higher of the registered in the greenhouse; plants grown in protected fields showed higher growth especially those grown in greenhouses; the biofertilizer applied to the soil increased the N, P2O5 and K2O concentration in the leaf dry matter and the 60% dose was the one that provided the best nutritional adequacy for the plant; the fruit weight, the fruit yield per plant and the fruit yield increased with the growth of biofertilizer dose applied to the soil and showed greater increases in plants grown on trellis; cultivation under greenhouse conditions allowed increased productivity and diseases more efficient control, especially rust; yields decreased in the second and third cycles due to LAI increase observed in these two cycles compared to the first one.
Este trabalho teve como objetivo identificar tÃcnicas de cultivo com biofertilizante em ambiente protegido e a campo aberto com a cultura do figo em Limoeiro do Norte, CE, contemplando trÃs ciclos de produÃÃo, no perÃodo de outubro de 2010 a dezembro de 2011. O delineamento experimental foi o de blocos ao acaso, com parcelas subdivididas, com 4 repetiÃÃes. Os tratamentos consistiram da combinaÃÃo de 03 ambientes de cultivo, estufa (EST), latada (LT) e campo aberto (CA), que constituÃram as parcelas, com 4 tratamentos nas sub parcelas, constituÃdos de 04 concentraÃÃes (biofertilizante:Ãgua) na seguinte forma: T0 (0% de biofertilizante : 100% de Ãgua); T1 (20% de biofertilizante : 80% de Ãgua); T2 (40% de biofertilizante : 60% de Ãgua); T3 (60% de biofertilizante : 40% de Ãgua), onde aplicaram-se 3,0L da soluÃÃo biofertilizante por planta a cada 15 dias. Os dados foram submetidos à anÃlise de variÃncia e quando significativos pelo teste F, realizaram-se testes de mÃdias pelo teste de tukey a 5% de probabilidade e os referentes aos nÃveis de biofertilizante foram submetidos à anÃlise de regressÃo. Os teores de N na matÃria seca foliar aumentaram em 19%, 21% e 11%, com o crescimento das doses de biofertilizante, para os trÃs ciclos, respectivamente. Quanto ao P e o K os incrementos foram de 25,0 %, 7,0 % e 13 % e de 12,6%, 10,5% e 29,4%, respectivamente, na sequÃncia dos ciclos. No primeiro ciclo nÃo houve diferenÃa significativa entre as produtividades mÃdias de 10,44 e 10,14 t ha-1 verificadas na LT e na EST, respectivamente, mas que foram estatisticamente superiores à obtida em CA com 9,62 t ha-1. No segundo ciclo o ambiente nÃo influenciou significativamente a variÃvel produtividade. Jà no terceiro ciclo a LT apresentou rendimento de fruto de 6,47 t ha-1, significativamente superior aos detectados na EST e a CA com 4,37 e 3,81 t h-1, respectivamente. Quanto ao rendimento total nÃo houve diferenÃa significativa entre as mÃdias 18,83 e 18,27 t ha-1 desta obtidas na EST e a CA, respectivamnte, contudo, foram estatisticamente inferiores à obtida na LT, com 22,49 t ha-1. Com base na anÃlise dos trÃs ciclos, pode-se afirmar, como conclusÃo geral, que hà viabilidade de cultivo orgÃnico de figo com biofertilizante em ambiente protegido de baixo custo, tipo latada, no semiÃrido. E como conclusÃes especÃficas podem-se afirmar que: a temperatura mÃxima do ar no ambiente latada à inferior em atà 3,1 oC em relaÃÃo a estufa, mas superior em atà 1,7 oC à observada a campo aberto; a umidade relativa do ar no ambiente campo aberto à superior em atà 4,1% em relaÃÃo a estufa, e em atà 3,7 % à observada na latada. O consumo de Ãgua na latada representou 87,3% do registrado a campo aberto, mas foi superior em 16,4% ao registrado na estufa; plantas cultivadas nos ambientes protegidos apresentaram maior crescimento com destaque para as cultivadas em estufa; o biofertilizante aplicado ao solo incrementou a concentraÃÃo de N, P2O5 e K2O na matÃria seca foliar e a dose de 60% foi a que proporcionou a melhor adequaÃÃo nutricional à planta; o peso de fruto, a produÃÃo de fruto por planta e o rendimento de fruto, aumentaram com o crescimento da dose de biofertilizante aplicada ao solo e apresentaram maiores incrementos em plantas cultivadas na latada; o cultivo sob condiÃÃes de ambiente protegido possibilitou o aumento da produtividade e o controle mais eficiente de doenÃas, especialmente a ferrugem; as produtividades diminuiram no segundo e no terceiro ciclos em funÃÃo do aumento do IAF observado nestes dois ciclos em relaÃÃo ao primeiro.

Conselho Nacional de Desenvolvimento Científico e Tecnológico
Pesticides of different chemical and toxicological classes are used to control the pest attack on crops. However, treatment with these substances leave residues in the environment or the product itself, when used extensively, disregarding the ceilings set by law. The materials conventionally used in steps of extraction and preconcentration of pesticides in environmental matrices have a limited adsorption capacity or have low selectivity for specific analytes. Therefore, this study investigated the feasibility of metal-organic materials (MMO) as an alternative sorbent for the extraction of contaminants in papaya (Carica papaya). Such compounds form a new class of hybrid materials organic-inorganic porous stable, ordered, high surface area, which enables its application as pre-concentrators analytes and also in gas storage, molecular recognition and adsorption. The molecular formulas suggested for metal-organic materials were synthesized ∞[(La0,9Eu0,1)2(DPA)3(H2O)3], for the heteronuclear compound, and ∞[Eu(C14H4O8)(C2O4)3(H2O)2] for the homonuclear compound. The ligand coordination Na4ntc with Eu+3 ion in the homonuclear material occurred through the oxygen atoms of carboxylate groups attached to aromatic ring. In the case of heteronuclear compound, the ligand is coordinated to the metals H2DPA from the carboxylate oxygen atoms and nitrogen of pyridine. The luminescence spectroscopy studies of metal-organic material ∞[Eu(C14H4O8)(C2O4)3(H2O)2], indicated the presence of a single site symmetry around the lanthanide ion. Since the emission spectrum of ∞[(La0,9Eu0,1)2(DPA)3(H2O)3] indicated that the first coordination sphere of the metal has a low point group symmetry and has a single species broadcaster. According to data from X-ray diffraction single-crystal heteronuclear compound crystallized in monoclinic system with space group P21/c. The material formed a microporous threedimensional structure with channels that propagate along the crystallographic c axis. The extraction method developed enabled recovery of the pesticide pyrimethanil (52%). The elution solvent was chosen as ethyl acetate and the ratio matrix: adsorbent used was 1:3 (m / m).
Pesticidas de diferentes grupos químicos e classes toxicológicas são empregados para controlar o ataque das pragas às culturas. Porém, o tratamento com estas substâncias deixa resíduos no ambiente ou no próprio produto, quando utilizado de maneira extensiva, desrespeitando os limites máximos estabelecidos pelas legislações. Os materiais convencionalmente utilizados nas etapas de extração e pré-concentração de pesticidas em matrizes ambientais apresentam uma capacidade de adsorção limitada ou tem baixa seletividade para analitos específicos. Diante destes dados, este trabalho teve como meta básica estudar a viabilidade de materiais metal-orgânicos (MMO) como um sorvente alternativo para a extração de contaminantes em mamão (Carica papaya). Tais compostos formam uma nova classe de materiais híbridos porosos orgânico-inorgânico estáveis, ordenados e com alta área superficial, o que possibilita sua aplicação como pré-concentradores de analitos e também em estocagem de gás, reconhecimento molecular e adsorção. As fórmulas moleculares sugeridas para os materiais metal-orgânicos sintetizados foram ∞[(La0,9Eu0,1)2(DPA)3(H2O)3], para o composto heteronuclear, e ∞[Eu(C14H4O8)(C2O4)3(H2O)2], para o composto homonuclear. Através dos dados dos espectros de IV a coordenação do ligante Na4ntc com o íon Eu+3 no material homonuclear ocorreu através dos átomos de oxigênio dos grupos carboxilatos ligados ao anel aromático. No caso do composto heteronuclear, o ligante H2DPA se coordenou aos metais a partir dos átomos de oxigênio dos carboxilatos e do nitrogênio do anel piridínico. Os estudos de espectroscopia de luminescência do material metal-orgânico [Eu(C14H4O8)(C2O4)3(H2O)2]∞, indicam a presença de um único sítio de simetria em torno do íon lantanídeo. Já o espectro de emissão do ∞[(La0,9Eu0,1)2(DPA)3(H2O)3] evidenciam que a primeira esfera de coordenação do metal possui grupo pontual de baixa simetria e a apresenta uma única espécie emissora. Os dados de difração de raios-X de monocristal o composto heteronuclear indicam que a cristalização ocorre em sistema monoclínico com grupo espacial P21/c. O material formou uma estrutura tridimensional com canais microporosos que se propagam ao longo do eixo c cristalográfico. O método de extração desenvolvido possibilitou recuperação do pesticida pirimetanil (52%). O solvente de eluição escolhido foi o acetato de etila e a proporção matriz:adsorvente utilizada foi 1:3 (m/m).

L’insufficienza cardiaca è una sindrome di grande rilevanza clinica nella quale il muscolo cardiaco non è in grado di pompare il sangue efficacemente nel corpo. Per il trattamento dei pazienti si ricorre, principalmente, ai glicosidi cardiaci o digitalici come la digossina, che svolgono la loro azione inibendo la pompa Na+/K+ e, conseguentemente, aumentando la concentrazione del calcio, favorendo una migliore contrazione. Il calcio viene però accumulato in alte concentrazioni nella cellula provocando nei pazienti cronici aritmie che possono portare, comunque, alla morte del paziente. Pertanto, lo scopo di questa tesi tesi è stato quello di sintetizzare nuovi candidati farmaci (drug leads) per il trattamento dell’insufficienza cardiaca che abbiano la stessa attività dei digitalici con indice terapeutico maggiore e minor tossicità, garantendo l’assenza di aritmie nei pazienti cronici. Il disegno delle molecole realizzate durante il periodo di tesi è partito dalla struttura chimica della molecola istaroxime: questo composto è attualmente in fase clinica III come farmaco cardiaco. Istaroxime è in grado di inibire efficacemente la pompa Na+/K+ evitando, allo stesso tempo, la comparsa di aritmie grazie alla sua azione stimolante su SERCA-2°, una proteina di membrana in grado di sequestrare il calcio citosolico durante il rilassamento cardiaco. Lo studio dei metaboliti di istaroxime ha portato all’identificazione di una molecola in cui il gruppo amminico della catena sull’ossima è stato ossidato a carbossile. Questa molecola, cosiddetta “PST3093”, è in grado di attivare selettivamente SERCA-2a senza inibire, invece, la pompa Na+/K+. Attualmente, questo composto costituisce l’unico candidato farmaco in grado di stimolare il rilassamento cardiaco evitando la comparsa di aritmie. Tuttavia, sia istaroxime che il suo metabolita presentano delle criticità legate alla presenza della funzionalità ossima, a cui è associata una componente genotossica nei trattamenti cronici. Pertanto, sono stati sintetizzate due classi di molecole, la prima ispirata a PST3093 in cui il gruppo funzionale responsabile della tossicità è stato rimosso. Queste molecole sono caratterizzate da un innovativo meccanismo di azione, in grado di stimolare efficacemente e selettivamente la proteina SERCA2a. In questa sezione sono state identificate due molecole come possibili candidati farmaci con e si sta procedendo con il loro sviluppo clinico. Il loro meccanismo di azione unico e non noto in letteratura, rappresenta un passo avanti verso lo sviluppo di nuove terapie contro l’aritmia. Nella seconda sezione, invece, i composti sintetizzati sono ispirati ad istaroxime. Qui, per evitare la degradazione dell’ammina primaria ad acido carbossilico, è stata costruita una libreria di composti caratterizzati dalla presenza di una ammina ciclica. In questa sezione i composti hanno dimostrato di possedere sia capacità inibitorie sulla pompa Na/K che stimolatorie su SERCA2a ma con minor potenza rispetto ad istaroxime. Tuttavia, questi composti non posseggono le tossicità intrinseca di istaroxime. Per proseguire nello sviluppo di queste molecole, nella terza sezione, realizzata in collaborazione con la Maastricht University, un sistema di drug delivery è stato realizzato grazie a processi di elettrofilatura. L’analisi preliminare di sistemi modello ha portato all’identificazione di sistemi polimerici composti da nanofibre ad alto grado di allineamento capaci di rilasciare nel medio studiato piccole quantità di molecole nel tempo, capaci di espletare la loro azione farmaceutica a livello locale, diminuendo i rischi associati al metabolismo di istaroxime. Questa sezione rappresenta l’inizio di uno sviluppo tecnologico volto al miglioramento della azione di istaroxime senza modificarne la struttura chimica.
Heart failure (HF) is one of the major causes of death in the world. Patients affected by HF presents a pathophysiological state in which the heart is not cable of pumping the blood efficiently in the body due to a loss of contractility of the myocardium, leading to a chronic condition in which oxygen and nutritional need of the body are not satisfied. The most important therapy involves inotropic agents, such as digitals glycosides, capable of improving the cardiac output. The most known digitalis glycoside is Digoxin extracted from Digitalis purpurea. Such compounds acts as inhibitor of the Na+/K+ pump, an active transporter capable of expelling Na+ from the cardiac cells introducing K+ against the natural gradient. The inhibition causes an accumulation of Na+ inside the cell. Sodium is so used from the Na+/Ca++ pump to introduce Ca++ inside the cell. The high concentrations of Ca++ accumulated inside cardiac cells induce contraction of the myocardium. However, digitalis compounds present a small gap between the active concentration and the toxic one. In fact, the over intracellular accumulation for long periods of Ca++ could leads to arrhythmic situations. At today is known only one compound able to stimulate a better contraction without causing arrhythmias, such molecule, called “Istaroxime”. Istaroxime is now under development for the treatment of acute decompensated heart failure. Detailed studies highlighted that a metabolite of Istaroxime, the so-called “PST3093”, acts as a pure activator of SERCA-2a, a protein able to sequestrate Ca2+ from the cytosol, without any action on Na+/K+ pump. PST3093 is the only example in the literature of a pure SERCA-2a activator, making an interesting case of study and a possible first in class drug. Despite the promising activity and the unique characteristic of this compound, there is still the main issue due to the genotoxicity of the carboxy-hydroxylamine formed after oxime degradation by metabolism. Within this thesis, the work can be roughly divided in three parts. In the first part, the work is focused on the development of stable derivatives of “PST3093”, able to maintain the unique stimulatory effect over Serca-2a but, at the same time, substituting the non-metabolically stable oxime. Two compounds were identified, and will be further developed as first-in-class drugs, able to efficiently stimulate the activity of Serca-2a with potential application as anti-arrhythmic drugs. The second section is focused on the creation of compounds inspired to Istaroxime, able to acts as Na+/K+ inhibitors and Serca-2a stimulator. Here we developed analogues of Istaroxime, lacking the risks associated to the oxime group. The third section was developed in MERLN institute of Maastricht University. In this part of the work was developed a drug delivery systems based on electrospun polymeric scaffolds, able to deliver locally the drug reducing its toxicity.

The aim of this dissertation was to understand lithogenic suspended sediment transport mechanisms and distribution in two river-influenced margins: The Cariaco Basin, Venezuela, and the Amazon Shelf, Brazil. Lithogenic sediment input in the Cariaco Basin is controlled by small mountainous rivers (SMR), while in the Amazon Shelf it is dominated by the Amazon River, the largest river in the world in terms of freshwater discharge (~20% of global riverine discharge). Optical transmissometer measurements were coupled with particulate organic matter (POM) observations to understand changes in the geochemical composition of suspended sediment and spatial/temporal distributions over the two regions of interest. In the Cariaco Basin sampling was conducted during the rainy seasons of September 2003, 2006 and 2008, and during the upwelling period (dry season) of 2009. Our results suggest that bottom nepheloid layers (BNL) originating at the mouth of the SMR discharging into the Cariaco Basin are a major delivery mechanism of terrigenous sediments to the basin's interior year-round. Intermediate nepheloid layers (INL) were also observed near the shelf break (~100m) and appear to effectively carry terrigenous material laterally from the shelf to deep waters, thereby providing a plausible supply mechanism of the terrestrial material observed in sediment traps, deployed >70 km offshore as part of the CARIACO Ocean Time-Series. These findings highlight the importance of small, local rivers in the Cariaco Basin as sources of terrestrial material. Indeed, the low isotopic composition of particulate organic carbon (δ13Corg, ~-30 - -24 ‰) carried by the BNL suggests that this material was continentally derived. BNL δ13Corg also changed with season, indicating that the geochemical composition of BNL reflects particle source. These nepheloid layers contained relatively low POM concentrations (average of 10%), agreeing well with published data, yet the fine sediment of the BNL may serve as mineral ballast, enhancing the sinking velocities of POC and thus increasing the efficiency of the biological pump in Cariaco. We suggest that during the transition between the upwelling and rainy season BNL deliver sediment to the deep Cariaco Basin in pulses. During upwelling, BNL are retained on the inner shelf by onshore Ekman transport associated with upwelling. The nepheloid layers are later released as the upwelling subsides; this, coupled with high river discharge rates, may explain the seasonal pulse of sediment observed at the end of the upwelling period (May) in the sediment trap array. The SMR in Cariaco also have the capacity to deliver large amounts of sediment to the Cariaco Basin during episodic events, such as earthquakes and floods. During September 2008 a sediment density flow was observed in the eastern Cariaco Basin, likely triggered by a magnitude 5.2 earthquake that occurred on August 11, 2008 off the city of Cumaná. Elevated suspended sediments near the bottom were observed at the mouth of the Manzanares Canyon (> 90 g m-2, over a depth of 165 m) and decreased to ~11 g m-2 (over a depth of 40 m) 42 Km away from the canyon's mouth at the CARIACO Ocean Time-Series site (10.5° N, 64.67° W). The sediment flux associated with this single event was ~ 10% of the total annual sediment flux that typically reaches the Cariaco Basin deep seafloor. Average carbon to nitrogen atomic ratios (C/N) as well as C and N isotopic composition confirm that most of the organic matter transferred by the sediment flow was of continental origin (C/N ratios of ~19.3, δ13C of -27.04 ‰, and δ15N of 6.83 ‰). The Manzanares River mouth is located at the head of the canyon, and likely supplies most of the fine grained sediments and fresh organic carbon that accumulate in the upper part of the canyon. This suggests that the canyon is an active depositional center, and its proximity to the Manzanares River and Cariaco Basin is critical for sediment supply offshore, which in turn can have a significant impact on the long-term sequestration of carbon into the deep basin. The nutrient and sediment biogeochemistry of the outer Amazon Shelf was studied in February-March 2010 to replicate observations made by the AmasSeds study in 1989-1991. These transects roughly corresponded to the AmasSeds Open Shelf (OS) and River Mouth (RM) transects. Onshore winds (~6 m s-1) contained the Amazon plume within ~120 Km of the coast; the plume was visible only in the mid-shelf stations located closest to the coast in the OS transect. Within the river plume, surface dissolved inorganic nutrient concentrations were near zero, except for silicates (4-6 μM). Coupled with oxygen supersaturation (AOU < 1), this suggested complete biological uptake of the major dissolved inorganic nutrients (N, P). Dissolved organic carbon (DOC) was also highest within the plume (average of 116 μM), decreasing to ~73 μM in oceanic waters. Total suspended solids (TSS) in surface waters within the plume were ~1-1.5 mg l-1, decreasing to ~0.2-0.3 mg l-1 in all other sampled stations both over the shelf and in deeper waters. TSS were highest within BNL (22-33 mg l-1) observed over the inner shelf; BNL were not observed outside the area of the Amazon plume. Suspended particulate organic carbon (POCsusp) showed a depleted δ13C isotopic signal (~-25 ‰ to -28 ‰) in surface and bottom waters, suggesting terrestrial provenance. Within the BNL, %POC was low (0.6-0.9%, as compared to 7-18% in surface waters), showing extensive and rapid decomposition of organic matter over the shelf. Atomic C/N ratios in particulate organic matter both in surface waters and within BNL were relatively close to Redfield's (8-14) and relatively stable over the area sampled. Particulate atomic organic carbon vs. particulate organic phosphorous (POC/POP) ratios were also low within the BNL (~110) and increased offshore (>500), suggesting a direct input of particulate P from the Amazon River or from reworked surface sediments. The fraction of POC in surface sediments was also low (0.73 ±; 0.56%; N = 5) and relatively uniform across the region sampled. We estimated instantaneous fluxes of 38.7 metric tons TSS s-1, 0.24 metric tons POC s-1 and 6.42 x 10-3 metric tons POP s-1 northwestward over an area extending between ~50 Km and 120 Km offshore. Our TSS estimates are 30% lower than those calculated by Nittrouer et al. (1986) during peak discharge of the Amazon. We also calculated that some 1.50 Tg yr-1 of DOC were being flushed northwestward along the outer shelf annually, which represent ~6% of the total DOC transported by the Amazon. By analyzing these two geographical settings it was possible to compare and contrast transport mechanisms of continentally-derived material and establish the relative importance of each mechanism in their different environment. There is still much to be understood regarding BNL in the Cariaco Basin, such as their role within the Manzanares Submarine Canyon with regards to sediment contribution and deposition. Additionally, during the last 30 years, anthropogenic influences on the small rivers around the Basin have significantly altered the drainage and sediment loads, yet reliable data to quantify the level of influence and change over time are not available. We need a better understanding of the natural variability of these small, tropical fluvial systems, trends and impact of episodic events, to better interpret the climate record stored at the bottom of the basin and predict future ecosystem changes in the region. In the Amazon Shelf, more accurate estimates of DOC, POC and POP fluxes northwestward are warranted. The magnitude of the Amazon River discharge dampens changes that have occurred in the last 20 years within the Amazon Basin, suggesting that historic Amazon Shelf sediment and carbon estimates are still valid. The data presented here adds to the growing body of literature that highlights the significance of river-influenced continental margins as sites of organic carbon deposition, remineralization export and sequestration.

At the Castle Creek study area, levee deposits are well-exposed over an area of ~2.6 km wide and ~90 m thick. This provides an opportunity to describe their lateral and vertical lithological changes, and accordingly details about their reservoir geometry and stratal continuity. Here, levee deposits are divided vertically into packages, each consisting of a sand-rich lower part overlain sharply by a mud-rich upper part. Each lower part displays a consistent thickening then thinning trend laterally away from its genetically related channel. The characteristics of these packages suggest that they were controlled by recurring changes in the structure of channellized flows, which in turn was controlled by grain size and grain sorting. This ultimately was controlled by short-term changes in relative sea level. Moreover, some mud- and sand-rich strata are rich in residual carbon suggesting that mid-fan levees can serve as source rocks for hydrocarbon generation, and also reservoirs.

Made available in DSpace on 2016-04-28T14:58:58Z (GMT). No. of bitstreams: 1 2007- Luiz Aurelio Peres Martelleto.pdf: 2127585 bytes, checksum: 81c879a6537f8e53170021781a03c57a (MD5) Previous issue date: 2007-01-19
A study was conducted at the Integrated System of Agroecological Production (a Research Farm, located in Serop?dica, State of Rio de Janeiro), to evaluate the cycle development and the agronomic performance of papaya under organic management, comparing two ways of growing the plant (with and without trunk bifurcation) in three types of protected environment. Papaya seedlings, cv. Baixinho de Santa Am?lia ("Solo" group), were transplanted to contiguous structures as follows: (a) greenhouse (plastic covering), (b) shadowed greenhouse ('sombrite' net - 30%, over the plastic sheet), and (c) screenhouse ('sombrite' net - 30%, exclusively), side-by-side to an area for papaya cultivation under natural conditions. The greenhouse lateral and frontal parts were protected with anti-aphid screen. In these four treatments, 50% of the hermaphrodite plants were submitted to an incision of the apical bud, soon after sex determination. For a 12 months period of cultivation: temperature, relative air humidity and light radiation were measured in each environment. Regarding to development of papaya: plant height, basal trunk diameter, number of leaves per plant, foliar area, and periods of time to complete phases of the cycle were determined. A study of floral biology and of fruits characteristics also corried out. Cultural practices agreed with technical rules of organic agriculture. For comparisons among differents environments, the jointly experiment analysis" method was adopted. Trunk bifurcation reduced plant height thus extending the time period of papaya production cycle under covered environments. However, there was negative influence in number and average weight of harvested fruits. In the greenhouses (plastic and plastic plus sombrite coverings) there were increases with respect to: plant height, number of leaves, foliar area and time- life of leaves in the plant. In addition, there were reductions in the incidence of black spot (leaves and fruits) and of fruit skin freckles. In the shaded environments, percents of pentandric and carpeloid fruits were lower, opposite to that of estaminate flowers. In the screnhouse (only sombrite net), a decreased proportion of fruits reaching commercial standart occurred. In the greenhouse (plastic covering), other benefitial effects were noticed, such as: anticipation of flowering and fruit harvesting, due to the acceleration of fruit ripening in the plant. Although an increase in the number pentandric, carpeloid and deformed, fruits was observed cultivation in the greenhouse also induced a highly significant increment in yield of marketable fruits (around 67%) compared with the natural environment. The results obtained indicated potential for organic papaya cultivation under greenhouse conditions, as judging by the expressive gain in productivity and plant health, besides the acceleration of the cycle.
O estudo foi conduzido no Sistema Integrado de Produ??o Agroecol?gica - S.I.P.A. (Fazendinha Agroecol?gica Km 47), situado em Serop?dica,RJ, tendo como objetivos acompanhar o desenvolvimento do ciclo e avaliar o desempenho agron?mico do mamoeiro sob manejo org?nico, comparando duas formas de condu??o da planta (com e sem bifurca??o do tronco), em ambiente natural e em tr?s tipos de ambiente protegido. Para tanto, mudas de mamoeiro da cultivar Baixinho de Santa Am?lia, grupo Solo , foram transplantadas para tr?s estruturas cont?guas: (a) estufa (cobertura de pl?stico), (b) estufa sombreada (cobertura adicional de sombrite 30% sobre o pl?stico) e (c) telado (cobertura de sombrite 30%); estabelecendo-se, ao lado, uma ?rea de cultivo em ambiente natural. Nas estufas (a e b), as laterais e frentes foram revestidas com tela anti-af?dica. Nesses quatro tratamentos, 50% das plantas hermafroditas tiveram o tronco bifurcado por meio de incis?o da gema apical, logo ap?s a determina??o do sexo. Registraram-se, ao longo de 12 meses de cultivo: temperatura, umidade relativa do ar e luminosidade em cada ambiente. Quanto ao desenvolvimento do mamoeiro: altura da planta, di?metro do tronco, n?veis de enfolhamento, ?rea foliar e per?odos de tempo para cumprimento de fases do ciclo, foram monitorados. Realizou-se, ainda, estudo da biologia floral e das caracter?sticas dos frutos produzidos. Os tratos culturais empregados obedeceram ?s normas t?cnicas da produ??o org?nica. Para compara??o entre ambientes de cultivo, adotou-se o m?todo da an?lise conjunta de experimentos . A bifurca??o do tronco reduziu a altura da planta prolongando o cultivo do mamoeiro nos ambientes cobertos. No entanto, teve influ?ncia negativa no n?mero e no peso m?dio dos frutos. Nas estufas (coberturas de pl?stico e de pl?stico mais sombrite) houve aumentos em rela??o a: altura da planta, n?vel de enfolhamento, ?rea foliar e sobrevida das folhas. Por outro lado, ocorreram redu??es da incid?ncia da var?ola (folhas e frutos) e da mancha fisiol?gica nos frutos. Nos ambientes sombreados, os percentuais de frutos pent?ndricos e carpel?ides foram mais baixos, ao contr?rio de flores estaminadas. No telado, registrou-se menor propor??o de frutos atingindo padr?o comercial. Na estufa, ocorreram benef?cios do ponto de vista agron?mico, destacando-se: antecipa??o do florescimento e do in?cio da colheita, al?m da acelera??o do amadurecimento dos frutos na planta. Embora tenha havido aumento do n?mero de frutos pent?ndricos, carpel?ides e aplastados, o cultivo na estufa proporcionou aumento altamente significativo da produ??o de frutos comercializ?veis (em torno de 67%) em compara??o ao ambiente natural. Os resultados obtidos indicaram potencial para o cultivo org?nico do mamoeiro sob condi??es de estufa, em fun??o dos ganhos expressivos de produtividade e sanidade, al?m de precocidade e acelera??o da colheita.

In this Thesis we discuss the effects of specific chemical functional- ization and partial/complete atomic substitution on the electronic (ground-/excited-state) and charge-transport properties of small or- ganic compounds of interest for molecular electronics. In particular, we considered several Polycyclic Aromatic Hydro- carbons (PAHs) with different morphologies (small-compact, com- pact, angular and linear). For these molecules we study the effects of complete substitution of the peripheral H atoms with halogens (F and CL), the functionalization with Triisopropylsilylethynil (TIPS) group, and the partial substitution with chalcogen (S in particular) atoms on several physical properties. In the first part of this work we report a systematic comparative study on dibenzo[b,def]chrysene (angular) and dibenzo[def,mno]chrysene (compact) polyaromatic hydrocarbons and their bis-triisopropylsilylethynyl (TIPS)-functionalized and per- halogenated (F, Cl) counterparts. We used all-electrons density functional theory(DFT)and time- dependent DFT(TDDFT)to quantify the effects of morphology and chemical modifications ondifferent physical observables, namely electron affinity,ionization energy,quasi-particleenergy gap,optical absorption,excitonbindingenergy, and molecular reorganization energies for holesand electrons.For thispart of thework we used the hybrid exchange-correlation functional B3LYP in conjunction with a Gaussian localized basis-set.This adopted combination functional/basis-set has proven to yield good results for polyaromatic hydrocarbons and derivatives. In the second part of the work we used the same theoretical frame- work (DFT and TDDFT), to study the electronic, optical, and charge- transport properties of the hexathiapentacene (HTP) molecule. HTP is a derivative of pentacene (PNT) obtained by symmetric substi- tution of the six central H with S atoms. We discuss in a compar- ative way the key molecular properties of HTP and PNT. In par- ticular, electron affinities, ionization energies, quasi-particle energy- gaps, optical absorption spectra, exciton binding energies, and re- organization energies for holes and electrons are calculated for the molecules and compared with the corresponding results for PNT, as well as with the available experimental data. The DFT and TDDFT results are also validated by performing many-body perturbation theory calculations within the GW and Bethe-Salpeter equation for- malisms. In addition, for the crystal structures of PNT and HTP we perfomed DFT-based calculations using a pseudopotentials+plane- waves formalism and adopting the PBE exchange-correlation func- tional empirically corrected in order to take properly into account dispersive interactions.

Submitted by Marlene Santos (marlene.bc.ufg@gmail.com) on 2017-10-02T17:16:52Z No. of bitstreams: 2 Dissertação - Jordana de Jesus Santos Pereira - 2017.pdf: 2005975 bytes, checksum: 1d53cfdb916b2f188eb7ed3980d23ed4 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)
Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-10-03T13:19:46Z (GMT) No. of bitstreams: 2 Dissertação - Jordana de Jesus Santos Pereira - 2017.pdf: 2005975 bytes, checksum: 1d53cfdb916b2f188eb7ed3980d23ed4 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)
Made available in DSpace on 2017-10-03T13:19:46Z (GMT). No. of bitstreams: 2 Dissertação - Jordana de Jesus Santos Pereira - 2017.pdf: 2005975 bytes, checksum: 1d53cfdb916b2f188eb7ed3980d23ed4 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-08-29
Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq
The fig (Ficus carica L.) is a fruitful species with great worldwide expansion, since it is considered a species of temperate climate, but that presents / displays good adaptation to a great diversity of climates and solos. Among the phytosanitary problems occurring in ficiculture, nematodes are the most important. The objective of the present study is to evaluate the influence of the application of different doses of organic compost and the intercropping with Crotalaria spectabilis on nematode population density and fig yield. The experiment was conducted in the orchard already installed, from September of the year 2015 to September 2016. The plants were arranged in a spacing of 4 m between rows and 2 m between plants. The treatments were five doses of organic compound, having as reference the recommended amount of nitrogen for the fig tree crop, combined with the presence or absence of Crotalaria spectabilis in a consortium. Five phytoematoid species were identified in the orchard, being Meloidogyne incognita, Pratylenchus sp., Helicotylenchus sp., Criconemoide sp. and Rotylenchulus sp .. M. incognita was present in higher population densities and with dominance over 90% in the three evaluation periods. This species was not affected by the doses of organic compound nor by the use of C. spectabilis. The productivity of the fig trees increased with the increase of the dose of applied organic compound.
O figo (Ficus carica L.) é uma espécie frutífera com grande expansão mundial, pois é considerada uma espécie de clima temperado, mas que apresenta boa adaptação a uma grande diversidade de climas e solos. Dentre os problemas fitossanitários ocorrentes na ficicultura, o de maior importância são os nematoides. O objetivo do presente estudo é avaliar a influência da aplicação de diferentes doses de composto orgânico e da consorciação com Crotalaria spectabilis sobre a densidade populacional de nematoides e a produtividade de figueira. O experimento foi conduzido no pomar já instalado, a partir de setembro do ano de 2015 até setembro de 2016. As plantas estavam dispostas em um espaçamento de 4 m entrelinhas e 2 m entre plantas. Os tratamentos foram cinco doses de composto orgânico, tendo elas como referência a quantidade recomendada de nitrogênio para a cultura da figueira, combinadas com a presença ou a ausência de Crotalaria spectabilis em consórcio. Foram identificados cinco espécies de fitonematoides no pomar sendo, Meloidogyne incognita, Pratylenchus sp., Helicotylenchus sp., Criconemoide sp. e Rotylenchulus sp.. M. incognita apresentou-se em maiores densidades populacionais e com dominância acima de 90% nas três épocas de avaliação. Esta espécie não foi afetada pelas doses de composto orgânico nem pelo uso de C. spectabilis. A produtividade das figueiras aumentou com o aumento da dose de composto orgânico aplicado.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-04-05T11:16:21Z No. of bitstreams: 1 2016 - Nilson Costa Rezende.pdf: 1161142 bytes, checksum: efc8ca5f40de89a345030c29f9450223 (MD5)
Made available in DSpace on 2017-04-05T11:16:21Z (GMT). No. of bitstreams: 1 2016 - Nilson Costa Rezende.pdf: 1161142 bytes, checksum: efc8ca5f40de89a345030c29f9450223 (MD5) Previous issue date: 2016-02-18
The study was conducted at the Integrated State Center for Rural Education - CEIER, in ?guia Branca, the Northwest Region of the Esp?rito Santo. The objective of this study was to evaluate the vegetative and reproductive behavior of papaya plant "Golden Sunrise", for different densities of cultivation under organic production system. The design chosen to effect the implementation of the experiment was randomized blocks, with six treatments (1667, 2167, 2667, 3167, 3667 and 4167 plants.hectare-1) and four replications (blocks). The density plantas.hectare 1667 was adopted as a control, the traditional crops of papaya 'Solo' group. Regarding the vegetative behavior planting density promoted in papaya plant: greater height, following a quadratic regression model; smaller diameter of the trunk, following a quadratic regression model; negatively affected the amount of leaves dropped by papaya plant, following a linear regression model; the presence of active photosynthetic leaves, following a quadratic regression model; the length of the leaves of papaya, following a quadratic regression model and leaf area, following a logarithmic regression model. In terms of floral behavior planting density did not affect the number of fruits and carpelloid pentandric and negatively influenced the number of perfect hermaphrodites fruits, following a quadratic regression model. Finally, for fruit production the density negatively influenced the number and weight of marketable fruit, following a quadratic regression model; positively the productivity of commercial fruits, following a quadratic regression model; the number of discarded fruits (out of commercial standard); negatively length and thickness of the fruit, following a quadratic regression model; negatively diameter of the fruit, following a logarithmic regression model and did not affect the number of seeds per fruit.
O estudo foi conduzido, no Centro Estadual Integrado de Educa??o Rural ? CEIER, situado no munic?pio de ?guia Branca, Regi?o Noroeste do Estado do Esp?rito Santo. O objetivo deste trabalho foi avaliar o comportamento vegetativo e reprodutivo do mamoeiro ?Sunrise Golden?, em rela??o a diferentes densidades de cultivo, sob sistema org?nico de produ??o. O delineamento escolhido para efetuar a implanta??o do experimento foi o de blocos ao acaso, com seis tratamentos (1667, 2167, 2667, 3167, 3667 e 4167 plantas.hectare-1) e quatro repeti??es (blocos). A densidade de 1667 plantas.hectare foi adotada como controle, tradicional nos cultivos de mamoeiro do grupo ?Solo'. Com rela??o ao comportamento vegetativo o adensamento de plantio promoveu nos mamoeiros: maior altura, seguindo um modelo de regress?o quadr?tico; menor di?metro do tronco, seguindo um modelo de regress?o quadr?tico; afetou de forma negativa a quantidade de folhas lan?adas pelos mamoeiros, seguindo um modelo de regress?o linear; a presen?a de folhas fotossinteticamente ativas, seguindo um modelo de regress?o quadr?tico; o comprimento das folhas dos mamoeiros, seguindo um modelo de regress?o quadr?tico e a ?rea foliar, seguindo um modelo de regress?o logar?tmica. Em termos de comportamento floral o adensamento de plantio n?o afetou o n?mero de frutos pent?ndricos e carpeloides e influenciou negativamente o n?mero de frutos hermafroditas perfeitos, seguindo um modelo de regress?o quadr?tico. Finalmente, para produ??o de frutos o adensamento influenciou negativamente o n?mero e o peso dos frutos comercializ?veis, seguindo um modelo de regress?o quadr?tico; positivamente a produtividade dos frutos comercializ?veis, seguindo um modelo de regress?o quadr?tico; o n?mero de frutos descartados (fora do padr?o comercial); negativamente o comprimento e espessura dos frutos, seguindo um modelo de regress?o quadr?tico; negativamente o di?metro dos frutos, seguindo um modelo de regress?o logar?tmico e n?o influenciou o n?mero de sementes por fruto.

This work is aimed at understanding the underlying processes of crystal growth in nanoporous materials at the molecular level utilising computational modelling. The coarse grain Monte Carlo program constructed over a number of works at the CNM has shown success in modelling cubic zeolite systems. The goal of this work is to adapt the program to deal with the complexities of a wide range of different crystal systems. There have been many studies of crystal growth and many problems solved. In zeolites, however, there are still a lot of questions to answer. Growth rates and activation energies for crystal growth processes in zeolites are some of the things that remain unsolved for zeolites. Coarse grain Monte Carlo modelling simplifies the problem and can provide an insight into the underlying processes that govern crystal growth. This study focused its energetics around the formation of stable closed cage surface structures deduced from careful study of the dissolution of zeolite L terraces. Two approaches from an energetic point of view were investigated during the course of this study. The first considered the energetics from an energy of attachment point of view whilst the second focused on the energy of destabilisation with respect to crystal bulk. In this study the crystal growth of the following systems were probed computationally: LTA, SOD, LTL, ERI, OFF. Both zeolite and MOF crystal systems were studied over the course of this work. The algorithm developed in study shows some potential in being able to give insight to experimental crystal growth chemists as to how changing the rates of growth of certain cage structures would affect the overall morphology of the crystal grown. They can then utilise their knowledge of how using certain cations or templates, for example, can alter the stabilisation of certain cage structures to in effect design crystals of desired properties.

Etude comparative des proprietes redox de treize clustres du co, rh, fe effectuee par des methodes electrochimiques (edt, coulometrie, voltamperometrie cyclique) associees a des methodes spectroscopiques (uv, rmn, rpe)

碩士
長庚大學
生化與生醫工程研究所
96
Carica papaya lipase is a naturally immobilized biocatalyst originated from plant. According to previous reports, this lipase has excellent enantioselectivity when carrying out the hydrolytic resolution of (R,S)-Naproxen esters. Therefore, the purpose of this research is aimed to use the lipase to perform transesterification resolution for a variety of recemic alcohols. We expect to screen suitable alcohol that can be resolved by using this lipase. When (R,S)-2-hydroxyl carboxylic acid esters were selected as the substrates to proceed lipase-catalyzed transesterification resolution, results revealed that the papaya lipase has better enantioselectivity for (R,S)-methyl 3-phenyllactate (E = 35.7). Changing of acyl donor to other vinyl esters of differ alkyl length and apolar solvent did not affect the enzyme performance. In comparison with the results of using the lipase pretreated with isopropyl ether or without the treatment, results revealed that the untreated papaya lipase has the best enantioselectivity for (R,S)-methyl 3-phenyllactate (E = 73.8). For (R,S)-2-hydroxyl carboxylic acid esters of different structures, the results revealed that the kinetic parameter of k2S are notably different for (R,S)-methyl 3-phenyllactate and (R,S)-ethyl 2-hydroxy-4-phenylbutyrate, i.e. 0.289 mmol/h g and 0.0117 mmol/h g, respectively. But the parameter Km of (R,S)-methyl mandelate was much higher than those of others, implying the very low affinity of the substrate to the lipase. The racemic alcohols of different structures were further selected as the substrates to perform the lipase-catalyzed transesterification resolution, with which the low reaction rate of (R,S)-methyl mandelate was elucidated.

碩士
南台科技大學
生物科技系
96
Branched chain fatty acids (BCFAs) are fatty acids substituted with alkyl groups. Many of them are chiral and therefore occur in two enantiomeric forms. Chiral BCFAs have been recognized as important flavour components. 2-Methylalkanoic acids and their derivates of high enantiomeric purities have also served as valuable synthetic intermediates. Lipase-catalyzed enantioselective esterification between (R,S)-2-methylalkanoic acids and n-butanol in organic solvents was developed to produce (S)-butyl 2-methylalkanoate. Partilly purified Carica papaya lipase (pCPL) was uesd to study the effect of organic solvents, reaction temperature, various methods of pretreated enzyme and different alkyl chain length of the chiral center of (R,S)-2-methylalkanoic acids. Isooctane was selected to use for optimally reaction medium. The increase of enantioselectivity of the pCPL was observed with the increase of the straight alkyl chain length of the chiral center of (R,S)-2-methylalkanoic acids from ethyl to pentyl in the esterification reaction. The enantioselectivity for pCPL was exceeded 100 for the kinetic resolution of (R,S)-2-methylheptanoic acid in isooctane, but for the the kinetic resolution of (R,S)-2-methylbutyric acid was only reached about 2 in isooctane. An effect of water activity (aw) on reaction rate and enatioselectivity was observed. The pCPL exhibited an optimal water activity for maximum esterrification rate VS,Q and enantioselectivity E at 0.06 and 0.33, resprctively. Measurements of the temperature dependence of E allows for separation of the enthalpic and entropic components of enantioselectivity. Changes in E with alkyl chain length of the chiral center of (R,S)-2-methylalkanoic acids were mainly caused by changes in the enthalpic component.

碩士
國立屏東科技大學
農園生產系所
103
Abstract Student ID: N10111005 Title of Thesis: Lethal Action of Potassium Fatty Acid Salts on Tetranychus kanzawai Kishida in Organic Cultivation of Carica papaya Total Page: 72 Name of Institute: National Pingtung University of Science and Technology Department of Plant Industry Graduate Date: 2015.07.15 Degree Conferred: Master Name of Student: Ming-Sheng Kuo Adviser: Chong-Ho Wang,Ph.D. Wen-Hua Chen, Ph.D. The Contents of Abstract in this Thesis: Papaya (Carica papaya L.), one important fruit tree in southern Taiwan, whose major breed falls on TAINUNG No. 2. Tetranychus kanzawai Kishida was the most hazardous mite. Farmers tend to use chemicals to tackle, but in the long run, papaya plants will have chemical resistance and the control of pesticide residue. What’s worse, the mite problem will be aggravated.Soap is saponified by natural oil. In addition, potassium soap, allowed to be used in organic farming in many countries. Different types of oils, however, contain different fatty acids.This study quantifies the lethal rates against Tetranychus kanzawai Kishida in different life stages, focusing on common oils which contain saturated and unsaturated fatty acids with 10-18 carbon atoms. It also tests cheaper oils sold in the market, such as coconut oil, palm oil, cotton seed oil and soybean oil. The saponified potassium salts of which lead to the lethal rates against Tetranychus kanzawai Kishida in different life stages. The test results serve as a reference for dealing with Tetranychus kanzawai Kishida while growing papaya.While conducting the indoor experiments, 2% potassium salts of fatty acids solution has distinctively different effect (within 72-hour lethal rate) on various life stages, the most significant lethal rate reaches up to 61.8-86.7%, falling on the shorter-chain saturated potassium salts of fatty acids with 10-12 carbon atoms and the long-chain unsaturated fatty acid potassium salt with 18 carbon atoms. Second to it, saturated fatty acid potassium salt with 14-18 carbon atoms has inferior lethal rate, 16.7-55.6% only. On the other hand, all kinds of fatty acid potassium salts have insignificant effect on the nonhatch rate of the eggs, below 28.9%. 2% liquid potassium soaps poses 58.9-83.3% lethal rate (within-72-hour) to various life stages, coconut oil potassium soap functions better, while palm oil potassium soap is inferior. All oil potassium soaps mentioned above have insignificant effect on the nonhatch rate of the eggs, below 14.4%. As for the field experiment, the 7-day-long prevention and cure rate is 56.5-66.2% when the liquid forms of the four oil potassium soaps are used on papaya plants once a week. And it accounts for 62.8-71.8% when the papaya plants are treated with the same liquid soaps once a week for 14 days. Coconut oil potassium soap and soybean oil potassium soap, exclusively, function better cure and prevention rate. Key words: Potassium fatty acid salts , Potassium soap, Tetranychus kanzawai Kishida , Carica papaya L.

碩士
南台科技大學
生物科技系
98
Branched chain fatty acids (BCFAs) are fatty acids substituted with alkyl groups. Many of them are chiral and therefore occur in two enantiomeric forms. Chiral BCFAs have been recognized as important flavour components. 2-Methylalkanoic acids and their derivates of high enantiomeric purities have also served as valuable synthetic intermediates. The purpose of this research is aimed to study the effect of organic solvents, reaction temperature, and different alkyl chain length of the chiral center of (R,S)-2-methylhexanoic acid esters by using partilly purified Carica papaya lipase ( pCPL ). The hydrolytic resolution of (R,S)-butyl-2-methylhexanoic ester via pCPL in different water-saturated solvents at 35℃ is employed as the model system for studying the effects of solvents on the lipase activity and enantioselectivity, where water-saturated isooctane are beneficial for giving better lipase activity and enantioselectivity. A comparison of pCPL enantioselectivity of alkyl chain straight for (R,S)-2-methylhexanoic acid esters of different indicates that (R,S)-butyl-2-methylhexanoic ester gives the best lipase activity. The thermodynamic analysis indicates that the enantiomer discrimination is driven by the difference of activation enthalpy ( ΔΔH ) and that of activation entropy ( ΔΔS ), yet the former is dominated for most of the reacting systems and has a good linear compensation relationship with the latter. Similar of the E value for different alkyl chain lengths of the chiral center of (R,S)-2-methylhexanoic acid esters were mainly attentted by both enthalpic and entropic changes.

博士
國立成功大學
化學工程學系碩博士班
93
(I) An analytical solution for the dynamic kinetic resolution of enzyme-catalyzed irreversible acyl transfer was employed to study the side-reaction effects on the yield and optical purity of the desired enantiomeric product. A lipase-catalyzed enantioselective thiotransesterification between (R, S)-naproxen 2,2,2-trifluoroethyl thioester and 4-morpholine ethanol with in situ racemization of (R)-thioester using trioctylamine as the racemization catalyst in isooctane was designed as a model system to compare the theoretical predictions. Under an initial water activity of 0.113, adding trioctylamine was found to have profound effects on the hydrolysis side-reactions, thus enhancing the chemoselectivity of the lipase to the alcohol in comparison with water. Theoretical analysis also indicated that the time-course yield and the enantiomeric excess for the desired enantiomer could be improved if the employed lipase had higher activity and enantioselectivity for the hydrolysis and esterification side-reactions, especially when the base had a similar racemization rate compared with the rate of the fast-reaction substrate. 　(II) A partially purified Carica papaya (pCPL) was explored as an effective biocatalyst for the esterification resolution of various (RS)-2-chlorophenoxypropionic acids (CPP) with trimethylsilylmethanol in anhydrous isooctane. In comparison with the performance of crude Candida rugosa lipase, pCPL has high E value (> 100) for 2-chloro and 4-chloro but not 3-chloro substituted CPP. More than an order-of-magnitude lower reactivity was obtained for the hindered substrate of (RS)-2-(2-chlorophenoxy)propionic acid. The thermodynamic analysis indicates that the enantiomeric discrimination for all reaction systems is mainly driven by the difference of activation enthalpy. A good enthalpy-entropy compensation effect between -ΔΔH and -ΔΔS is demonstrated and elucidated for the region-effect of substrates containing 2-, 3- or 4-chloro substituent. The kinetic analysis indicates that pCPL is inhibited strongly by the acid substrate, but to a less extent by the alcohol substrate. However with the penalty of decreasing pCPL activity, the E value in isooctane can be greatly enhanced when trimethylsilylmethanol concentration higher than 40 mM is employed.

Background There is a transition towards minimally invasive restorative techniques in restorative dentistry based upon reducing bacterial viability and encouraging remineralization of caries infected tissue. To improve the predictability of the antibacterial and remineralization potential of carious dentine by either the application of medicaments or placement of restorative materials that encourage remineralization would be a significant benefit in disease management. Materials and Methods An experimental model was developed using a chemostat for in vitro analysis of the effects of silver fluoride followed by potassium iodide (AgF/KI) and ozone treatment on non demineralized and demineralized dentine. Electron Probe Micro Analysis (EPMA) and Scanning Electron Microscopy (SEM) on the treated dentine were conducted to investigate ion transfer, and biofilm formation. Bacteria growth was measured by optical density. An in vitro caries model using a chemostat was developed to determine the ability of glass ionomer cement and composite resin to inhibit dentinal degradation in adjacent dentine and to measure ion exchange at the restorative interface. Tests were made to determine the bond strength between dentine and glass ionomer cement after application of silver fluoride to the surface of the dentine. Results S. mutans migrated through all dentine samples. Samples treated with AgF/KI had significantly lower optical densities than the corresponding controls. Optical density readings were significantly lower in demineralized dentine treated with AgF/KI than non demineralized dentine. There were lower but not significant differences in the optical density readings between ozonated and non ozonated dentine. An S. mutans biofilm covered all control discs. No biofilm was detected on discs treated with AgF/KI and these discs were significantly more resistant to further demineralization than the control discs. Detectable amounts of silver and fluoride were found up to 450 μm in the AgF and AgF/KI sections. Ozone infusion prevented S. Mutans and L. acidophilus biofilm formation on all the treated dentine samples, biofilm was present on all control specimens. There was calcium and phosphorus present in all auto cure glass ionomer cements to a depth beyond 50 microns. Aluminium and strontium ions were also present in dentine except strontium subjacent to Ketac Molar restorations. Fluoride uptake was significantly higher under glass ionomer cement restorations where the dentine was pretreated with AgF/KI compared to non treated specimens. Silver and iodine deposits were present in demineralized dentine treated with AgF/KI. Calcium and phosphorus levels up to 130 microns from the restorative interface were similar to non demineralized dentine adjacent to auto cure glass ionomer cements and half that adjacent to composite resin. There was significant surface degradation in auto cure glass ionomer cements compared to composite resin. Washing away the AgF/KI precipitate produced higher bond strengths to dentine than samples where the precipitate remained. Conclusions Under the conditions of these in vitro studies, the application of AgF/KI and ozone pharmacologically reduces the initiation and rate of dentine caries. Glass ionomer cements were shown to protect dentine from experimental carious degradation and assist with remineralization. AgF/KI application enhances remineralization beneath glass ionomers and does not interfere with bond strengths.
http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1344616
Thesis (Ph.D.) - University of Adelaide, Dental School, 2008

Background There is a transition towards minimally invasive restorative techniques in restorative dentistry based upon reducing bacterial viability and encouraging remineralization of caries infected tissue. To improve the predictability of the antibacterial and remineralization potential of carious dentine by either the application of medicaments or placement of restorative materials that encourage remineralization would be a significant benefit in disease management. Materials and Methods An experimental model was developed using a chemostat for in vitro analysis of the effects of silver fluoride followed by potassium iodide (AgF/KI) and ozone treatment on non demineralized and demineralized dentine. Electron Probe Micro Analysis (EPMA) and Scanning Electron Microscopy (SEM) on the treated dentine were conducted to investigate ion transfer, and biofilm formation. Bacteria growth was measured by optical density. An in vitro caries model using a chemostat was developed to determine the ability of glass ionomer cement and composite resin to inhibit dentinal degradation in adjacent dentine and to measure ion exchange at the restorative interface. Tests were made to determine the bond strength between dentine and glass ionomer cement after application of silver fluoride to the surface of the dentine. Results S. mutans migrated through all dentine samples. Samples treated with AgF/KI had significantly lower optical densities than the corresponding controls. Optical density readings were significantly lower in demineralized dentine treated with AgF/KI than non demineralized dentine. There were lower but not significant differences in the optical density readings between ozonated and non ozonated dentine. An S. mutans biofilm covered all control discs. No biofilm was detected on discs treated with AgF/KI and these discs were significantly more resistant to further demineralization than the control discs. Detectable amounts of silver and fluoride were found up to 450 μm in the AgF and AgF/KI sections. Ozone infusion prevented S. Mutans and L. acidophilus biofilm formation on all the treated dentine samples, biofilm was present on all control specimens. There was calcium and phosphorus present in all auto cure glass ionomer cements to a depth beyond 50 microns. Aluminium and strontium ions were also present in dentine except strontium subjacent to Ketac Molar restorations. Fluoride uptake was significantly higher under glass ionomer cement restorations where the dentine was pretreated with AgF/KI compared to non treated specimens. Silver and iodine deposits were present in demineralized dentine treated with AgF/KI. Calcium and phosphorus levels up to 130 microns from the restorative interface were similar to non demineralized dentine adjacent to auto cure glass ionomer cements and half that adjacent to composite resin. There was significant surface degradation in auto cure glass ionomer cements compared to composite resin. Washing away the AgF/KI precipitate produced higher bond strengths to dentine than samples where the precipitate remained. Conclusions Under the conditions of these in vitro studies, the application of AgF/KI and ozone pharmacologically reduces the initiation and rate of dentine caries. Glass ionomer cements were shown to protect dentine from experimental carious degradation and assist with remineralization. AgF/KI application enhances remineralization beneath glass ionomers and does not interfere with bond strengths.
Thesis (Ph.D.) - University of Adelaide, Dental School, 2008