Journal articles on the topic 'Carcase hygiene'
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Vanderlinde, Paul, Peter Horchner, Long Huynh, and Ian Jenson. "Microbiological Quality of Red Meat Offal Produced at Australian Export Establishments." Foods 11, no. 19 (September 27, 2022): 3007. http://dx.doi.org/10.3390/foods11193007.
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A national baseline study of offal hygiene was undertaken at 17 Australian export establishments. A total of 1756 samples of different offal types were analysed for aerobic plate count (APC), generic Escherichia coli, and coliform bacteria. Average APC values varied from 1.51 to 5.26 Log10 CFU/g, depending on species and offal type. The average APC on beef, sheep, lamb, and goat offal was 3.25, 3.38, 3.70, and 2.97 Log10 CFU/g, respectively. There is a small but significant difference in APC on offal sampled frozen (3.26 Log10 CFU/g) and offal sampled fresh (3.73 Log10 CFU/g). Escherichia coli prevalence on beef, sheep, lamb, and goat offal was 15.4%, 28.1%, 17.5%, and 39.3%, respectively. The number of E. coli on positive offal samples ranged from 1.42 to 1.82 Log10 CFU/g. While the quality of some offal approach that of muscle meat, the hygienic quality of red meat offal can be understood by considering the anatomical site from which it is harvested, the usual bacterial levels found at that site, the difficulty in hygienically removing the offal from the carcase, the process prior to packing, and the chilling method used.
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Tibulca, Dorin, Claudiu Dan Salagean, and Mirela Jimborean. "The Establish of the Coliforms/cm2 on the Area of Cattle Carcass Air Drying." Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Food Science and Technology 71, no. 1 (May 20, 2014): 23. http://dx.doi.org/10.15835/buasvmcn-fst:10122.
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Coliforms present in 1 cm2 of carcass surface shows the degree of contamination during slaughtering as well as the hygienic condition of the air, the slaughtering hall, the equipment getting in contact with the carcasses, of the utensils, operators’ work equipment, of the operators’ hygiene. The indicator is determined by inoculating microorganisms from the carcasses surface in nutritional and selective environments, followed by their placing under heat control and counting of the microorganisms.
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Kiermeier, Andreas, Joanne Bobbitt, Paul Vanderlinde, Glen Higgs, Andrew Pointon, and John Sumner. "Use of routine beef carcase Escherichia coli monitoring data to investigate the relationship between hygiene status of incoming stock and processing efficacy." International Journal of Food Microbiology 111, no. 3 (October 2006): 263–69. http://dx.doi.org/10.1016/j.ijfoodmicro.2006.05.006.
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Pointon, A., A. Kiermeier, and N. Fegan. "Review of the impact of pre-slaughter feed curfews of cattle, sheep and goats on food safety and carcase hygiene in Australia." Food Control 26, no. 2 (August 2012): 313–21. http://dx.doi.org/10.1016/j.foodcont.2012.01.034.
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STELLA, SIMONE, ERICA TIRLONI, EMANUELE CASTELLI, FABIO COLOMBO, and CRISTIAN BERNARDI. "Microbiological Evaluation of Carcasses of Wild Boar Hunted in a Hill Area of Northern Italy." Journal of Food Protection 81, no. 9 (August 17, 2018): 1519–25. http://dx.doi.org/10.4315/0362-028x.jfp-18-077.
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ABSTRACT This study evaluated the prevalence of potential pathogenic bacteria (mainly Campylobacter spp., but also Listeria monocytogenes and Salmonella) in wild boar (Sus scrofa) and the hygiene of carcasses of wild boar hunted in a hill area of northern Italy during a hunting season (October to December). In total, 62 animals were submitted to microbiological analyses of the tonsils (detection of Listeria spp. and Listeria monocytogenes), caecal content (detection of Salmonella and Campylobacter spp.), mesenteric lymph glands (detection of Salmonella), and carcasses. In addition to analyzing pathogen prevalence and carcass hygiene of these animals, we performed an enumeration of total viable count (TVC), Enterobacteriaceae, Escherichia coli, coagulase-positive staphylococci, and spores of sulfite-reducing clostridia. Influencing factors considered were sex, weight, and age of the animals and environmental temperature on the day of hunting. A high prevalence was observed for L. monocytogenes in tonsils (35.3%) and for Campylobacter spp. in caecal content (51.8%), whereas Salmonella enterica strains (mainly serovar Thompson) were only occasionally isolated (7% in caecal content and 3.5% in lymph glands). The prevalence of L. monocytogenes was influenced by animal age and environmental temperature. Campylobacter spp. were the only pathogens detected on the carcasses (16.7%). Carcasses were characterized by low levels of contamination: TVC, 3.21 ± 0.80 log CFU/cm2, Enterobacteriaceae, 1.32 ± 0.89 log CFU/cm2; E. coli, 1.31 ± 0.93 log CFU/cm2; and occasional detection of low counts of staphylococci and clostridia. TVC was positively influenced only by high environmental temperature, and higher Enterobacteriaceae counts were detected on heavy male carcasses than on females. The results confirmed the potential role of wild boars as reservoirs for the most important foodborne pathogens. But a low carcass contamination level is achievable if hunters are properly trained about hygienic carcass management and slaughtering procedures.
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Morgan, I. R., F. L. Krautil, and J. A. Craven. "Bacterial populations on dressed pig carcasses." Epidemiology and Infection 98, no. 1 (February 1987): 15–24. http://dx.doi.org/10.1017/s0950268800061677.
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SUMMARYSamples were collected at two abattoirs from four sites on pig carcasses as they were being placed in a chiller. Bacteriological examination showed that no single sampling site could be used to assess the microbiological status of pig carcass surfaces. Sampling from multiple sites on a carcass may be required to assess the degree of contamination by different bacteria. It is suggested that the hygiene of slaughtering and dressing of pig carcasses at an abattoir cannot be assessed on a single visit and that a number of visits are necessary to establish a hygiene pattern.
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LASTA, JORGE A., RICARDO RODRÍGUEZ, MARTA ZANELLI, and CARLOS A. MARGARÍA. "Bacterial Count from Bovine Carcasses as an Indicator of Hygiene at Slaughtering Places: A Proposal for Sampling." Journal of Food Protection 55, no. 4 (April 1, 1992): 271–78. http://dx.doi.org/10.4315/0362-028x-55.4.271.
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A sampling technique by which the whole carcass is rubbed with a polyurethane sponge was used to study bacterial status on 523 beef carcasses at six different slaughterhouses over four different years. Although some abattoirs were differentiated based upon the psychrotroph counts from their carcasses, effects on counts of visits and season of sample taking, as well as interaction year x abattoir found at the other plants were large enough to mask the abattoir effect. Mesophile counts were not consistent enough to discriminate abattoirs, while, Enterobacteria, total and fecal coliforms, and Staphylococcus aureus coagulase-positive organisms showed very low counts and did not set apart differences. A guideline to monitor beef carcass hygiene and indirectely the hygiene of the slaughtering practices through the psychrotroph counts is proposed. A two-kinds sampling plan is suggested with “right-incorrect” as levels of hygiene. A sample unit (n) of 10, an acceptance number of contaminated carcasses (c) of 3, and a count limit (m) of 103 CFU/cm2 are proposed. Under this guideline, a lot of carcasses will be deemed as hygiene lacking when 4 or more, out of 10 carcasses, yield counts of 103 CFU/cm2 or higher.
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Ivanovic, Snezana, M. Zutic, O. Radanovic, and S. Lilic. "Slaughterhouse: Slaughter place or source of contamination." Biotehnologija u stocarstvu 23, no. 3-4 (2007): 101–7. http://dx.doi.org/10.2298/bah0704101i.
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There are many possibilities of pig carcasses contamination by pathogenic microorganisms, particularly on the slaughter line. The aim of this paper was to affirm a hygienic level of the slaughter line by the enumeration of aerobic bacteria and enterobacteria. Samples were taken by both non-destructive and destructive methods from 4 place on the carcass of 5 pigs, weekly for 15 weeks. The results of examination has shown the hygiene of slaughter line had satisfied and acceptable level. Destructive method of samples was more susceptible than non-destructive method. The obtained results have interpreted according to regulation EC 2073/2005. .
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GIOMBELLI, AUDECIR, RICARDO CAVANI, and MARIA BEATRIZ ABREU GLORIA. "Evaluation of Three Sampling Methods for the Microbiological Analysis of Broiler Carcasses after Immersion Chilling." Journal of Food Protection 76, no. 8 (August 1, 2013): 1330–35. http://dx.doi.org/10.4315/0362-028x.jfp-13-004.
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Countries have different official programs and implement different sampling methods for the detection of Salmonella on poultry carcasses. In Brazil, a 25-g sample of skin and muscle excision (SME) from the wings, neck, and pericloacal parts is used; in the European Union (EU), a 25-g sample of neck skin (NSE) is used; and, in the United States, the whole carcass is rinsed with 400 ml of diluent (WCR). In the present study, these methods were evaluated to compare Salmonella occurrence and counts of hygiene indicator microorganisms (Escherichia coli, Enterobacteriaceae, and total viable count of aerobic mesophilic bacteria) using different carcasses from the same flock and also using different analytical units taken from the same carcass. Eighty flocks, with four broiler carcasses from each, were included in this study; three broilers were sampled according to protocols from Brazil, the EU, and the United States, and the last one by all three methods. SME, NSE, and WCR provided equivalent results (P > 0.05) for Salmonella detection on broiler carcasses when using different carcasses from the same flock and when using the same carcass. The predominant serovar was Salmonella Enteritidis. For the enumeration of hygiene indicator microorganisms, WRC provided higher counts than SME or NSE (P < 0.05), when using both the same or different carcasses. Therefore, it is possible to directly compare Salmonella results in poultry carcasses when using the methods recommended by the legislative bodies of Brazil, the United States, and the EU. However, WCR provides the best results for hygiene indicator microorganisms.
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ALGINO, R. J., G. A. BADTRAM, B. H. INGHAM, and S. C. INGHAM. "Factors Associated with Salmonella Prevalence on Pork Carcasses in Very Small Abattoirs in Wisconsin." Journal of Food Protection 72, no. 4 (April 1, 2009): 714–21. http://dx.doi.org/10.4315/0362-028x-72.4.714.
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The U.S. Department of Agriculture has expressed concern over Salmonella prevalence on pork carcasses. Our objectives were to survey the prevalence of Salmonella on pork carcasses in very small Wisconsin abattoirs, and identify processing conditions and indicator bacteria levels associated with reduced Salmonella prevalence. During April to July 2007, sponge samples were obtained from 181 pork carcasses at 10 Wisconsin abattoirs before carcass washing (carcass half A), and after washing and chilling and before fabrication (carcass half B). Each sample was categorized by whether the carcass was skinned, by wash-water temperature (7 to 43°C), and the duration (1 or 2 days), temperature, and percent relative humidity of chilling. Sponge samples were analyzed qualitatively for Salmonella and quantitatively for Escherichia coli, coliforms, Enterobacteriaceae, and aerobic plate count (APC). Salmonella prevalences on skinned and unskinned prewash carcasses were 11.7 and 8.3%, respectively. Corresponding values for chilled carcasses were 32.0 and 19.5% for 1-day chilled carcasses, and 11.4 and 14.7% for 2-day chilled carcasses. Lower Salmonella prevalence on prewash carcasses was significantly related to lower prewash carcass APC levels (odds ratio = 7.8 per change of 1.0 log CFU/cm2), while lower Salmonella prevalence on chilled carcasses was significantly related to 2-day chilling (odds ratio = 5.2), and chilled-carcass levels of coliforms, Enterobacteriaceae, and APC (odds ratio = 1.5 to 1.9 per change of 1.0 log CFU/cm2). Salmonella prevalence on chilled pork carcasses in very small Wisconsin plants could be reduced by chilling carcasses 2 days before fabrication and improving carcass-handling hygiene.
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Pyz-Łukasik, Renata, and Waldemar Paszkiewicz. "Hygiene assessment of sheep slaughter cycle." Bulletin of the Veterinary Institute in Pulawy 58, no. 2 (June 1, 2014): 243–46. http://dx.doi.org/10.2478/bvip-2014-0036.
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Abstract The purpose of the study was to determine microbial contamination of mutton carcass surface with regard to the number of the slaughtered animals. The total bacterial load and Enterobacteriaceae and enterococci counts were determined. Sampling for microbiological analysis as well as detection and enumeration of each microorganism group were performed according to the Polish Standards. No significant effect of the order of the slaughtering animals during the slaughter day on total bacterial count on mutton carcass surfaces was found. The overall bacterial contamination of mutton carcasses were between 1.0 × 103 cfu/cm2 (3.0 log - stage I) and 2.5 × 103 cfu/cm2 (3.4 log - stage III). No significant difference among the slaughter cycles, as indicated by total microbial numbers was observed. The obtained daily mean log values ranged from 4.7 × 102 (2.67 log) and 7.6 × 103 (3.88 log) cfu/cm2. The daily log mean values were lower than the maximal bacteria count (M) set out for hygiene standard of sheep slaughter process by the Commission Regulation 2073/2005. Bacteria belonging to the Enterobacteriaceae family were recovered from 21 (65.6%) samples while enterococci were identified in 28 (87.5%) samples. In most cases, significant differences in the level of contamination with bacteria isolated from the carcasses at each stage of a daily slaughter cycle, were not observed. At stage III, significantly higher levels of bacterial contamination (0.86 and 1.31 log cfu/cm2 respectively) were established as compared to stage I (0.37 and 0.58 log cfu/cm2 respectively). There were no Salmonella-positive samples determined. Importantly, the number of slaughtered animals during a slaughter day did not influence bacterial contamination on carcass surface if the successful application of HACCP control system was combined with the implementation of optimal sanitary supervision.
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Mwasinga, Wizaso, John B. Muma, Clovice Kankya, Chisoni Mumba, and Musso Munyeme. "Evaluation of Bacterial Contamination of Beef Carcasses in Namwala and Lusaka Districts of Zambia." University of Zambia Journal of Agricultural and Biomedical Sciences 5, no. 1 (April 1, 2021): 29–41. http://dx.doi.org/10.53974/unza.jabs.5.1.490.
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Abattoirs have been purported to be ideal areas were possible microbial contamination of meat products is likely to occur. Food of animal origin, mainly beef, has been identified as a source of dietary protein for humans albeit it being a source of food-borne diseases including zoonoses. This study was carried out to evaluate bacterial contamination and the risk factors associated with contamination of beef carcasses during processing. A total of four abattoirs were sampled within three months with one in Namwala and three in Lusaka districts. A total of 314 beef carcass surface swabs were obtained from the neck region by swabbing the carcasses, immediately after evisceration and after washing. Results The results of mean total viable counts (TVC) of carcass contamination were enumerated as the mean log from 4.7 Log10 cfu/cm2 in an abattoir where Hazard Analysis and Critical Control Points (HACCP) was practiced to 5.8 Log10 cfu/cm2 without HACCP. Bivariate analysis showed a significant difference in carcass contamination when town abattoirs (Lusaka) were compared with rural ones (Namwala); χ2 = 43.87, P < 0.0001. Multiple logistic regression analysis identified poor hygiene practices, the absence of antemortem inspection, and lack of Hazard Analysis and Critical Control Points (HACCP) implementation as significant factors associated with carcass contamination. Conclusion A high microbial load as determined by the Total Viable Count is an indicator that beef is being produced under poor abattoir hygiene conditions. Therefore, beef carcasses with high bacterial loads are potential sources of foodborne pathogens leading to foodborne disease and hence there is need for advocating for good hygiene practices in the abattoirs.
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CORRÒ, M., S. SALEH-MOHAMED-LAMIN, S. JATRI-HAMDI, B. SLUT-AHMED, S. MOHAMED-LEJLIFA, S. DI LELLO, D. ROSSI, A. BROGLIA, and L. VIVAS-ALEGRE. "A Preliminary Microbiological Assessment of Process Hygiene of Traditional Outdoor Camel Slaughter in Sahrawi Refugee Camps†." Journal of Food Protection 75, no. 10 (October 1, 2012): 1859–61. http://dx.doi.org/10.4315/0362-028x.jfp-12-132.
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The aim of this study was to investigate the hygiene performance of a camel (Camelus dromedarius) slaughtering process as carried out with the traditional method in the Sahrawi refugee camps located in southwestern Algeria. The camel slaughtering process in this region differs significantly from that carried out in commercial abattoirs. Slaughtering is performed outdoors in desert areas, and dehiding of the carcass is approached via the dorsoventral route rather than the classic ventrodorsal route. Samples were taken from 10 camel carcasses from three different areas: the hide, the carcass meat immediately after dehiding, and the meat after final cutting. Enterobacteriaceae counts (EC) were enumerated employing conventional laboratory techniques. Carcass meat samples resulted in EC below the detection limit more frequently if the hide samples from the same carcass had also EC counts below the detection limit. Because of the low number of trials, the calculation of statistical significance of the results was not possible. Further experimental research is needed in order to validate the results presented in this study. The comparison of the microbiological hygiene performance between dorsal dehiding and traditional ventral dehiding of slaughtered animals could serve to validate the hypothesis of the potential positive impact of the dorsal dehiding method in carcass meat hygiene.
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Camargo, Anderson, Marcus Cossi, Wladimir Silva, Luciano Bersot, Mariza Landgraf, József Baranyi, Bernadette Franco, and Nero Luís Augusto. "Microbiological Testing for the Proper Assessment of the Hygiene Status of Beef Carcasses." Microorganisms 7, no. 3 (March 19, 2019): 86. http://dx.doi.org/10.3390/microorganisms7030086.
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Microbiological testing is an important quality management tool in the food industry. In this study, the hygiene status of beef carcasses sampled in eight Brazilian slaughterhouses was assessed by enumeration of different hygiene indicator microorganisms, and a model to establish potential associations among these counts was proposed. The carcasses (n = 464) were surface sampled at four slaughtering steps (step 1: Hide after bleeding; step 2: Carcass after hide removal; step 3: Carcass after evisceration; step 4: Carcass after end washing) and subjected to a counting of mesophilic aerobes (MA), Enterobacteriaceae (EB), total coliforms (TC), and Escherichia coli (EC) using Petrifilm™ plates. Among the sampled beef carcasses (step 4), 32 (6.9%) and 71 (15.3%) presented counts above the microbiological criteria established by (EC) No. 1441/2007 for MA and EB, respectively. Thus, indicating that improvements in slaughter hygiene and a review of process controls are demanded in some of the studied slaughterhouses. The log count differences of EC, TC, and EB from MA were considered as response variables as a function of the slaughtering steps. Differential log counts changed consistently with the steps. The measurements, including the patterns in their inherently random variability, were fairly predictable from steps 1 and 4. The results indicated that differential log counts for TC and EC are not relevant, as their concentrations and random pattern can be inferred from counts of MA and EB. The proposed model can be used as a valuable tool for the design and adoption of feasible quality control programs in beef industries. The adoption of such a tool should have a positive contribution on consumers’ health and enhance product quality.
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GILL, C. O., and L. P. BAKER. "Assessment of the Hygienic Performance of a Sheep Carcass Dressing Process†." Journal of Food Protection 61, no. 3 (March 1, 1998): 329–33. http://dx.doi.org/10.4315/0362-028x-61.3.329.
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Swab samples were obtained from the surfaces of randomly selected carcasses passing through a sheep carcass-dressing process. A single sample was obtained from a randomly selected site on the surface of each carcass. Twenty-five such samples were collected at each of four stages in the process. The aerobio bacteria, coliforms, and Escherichia coli recovered from each sample were enumerated. Values for the mean log and standard deviation of each set of 25 log10 values were calculated on the assumption that the log values were normally distributed. The log of the arithmetic mean was estimated from the mean log and standard deviation values for each set. The results showed that bacteria, including coliforms that were largely E. coli, were deposited in high numbers during skinning operations, mainly on the butts and shoulders of carcasses. The mean numbers of coliforms and E. coli on carcasses were little affected by eviscerating and trimming operations, although they were redistributed from the sites they occupied after skinning. Total counts were redistributed and augmented by eviscerating and trimming operations. Washing reduced the log numbers of all of the bacteria by approximately 0.5. The general hygienic characteristics of the sheep carcass dressing process were similar to those of a previously examined beef carcass-dressing process.
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Ismanto, Arif, Try Julianda, and Mursidah. "ANALISIS SIKAP DAN KEPUASAN KONSUMEN TERHADAP ATRIBUT PRODUK KARKAS AYAM PEDAGING SEGAR DI PASAR TRADISIONAL KOTA SAMARINDA." Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) 8, no. 2 (September 1, 2018): 69. http://dx.doi.org/10.30862/jipvet.v8i2.34.
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This research aims to know the attitude of consumers, the level of satisfaction, and what product attributes that are considered consumers before buying the broiler carcass in traditional market of Samarinda City. The data retrieval research conducted during November 2016. The basic method of research is using descriptive method, and it’s implementation with the technique level. The location of the study was chosen intentionally in Samarinda City by taking 3 traditional markets as a sample. The determination of the number of samples is done proportionally and the technique of determining the sampling method by chance (Accidental sampling) with the number of samples of 50 respondents. The type of data used is primary data and secondary data with data collection techniques by observation, interview, and recording. The analysis used is Fishbein Multi Attribute attitude analysis and to analyze satisfaction using Customers Satisfaction Index. The result of the research based on analysis of Fishbein Multiatribut known to consumer attribute value toward color attribute 16.80, weight (16.48), aroma (15.76), skin hygiene (14.57) and price (13.11). Based on Costumer Satification Index the level of customer satisfaction on broiler carcass is 77.11%. Result of research based on analysis of Fishbein Multi Attribute known consumer attitude toward carcass is “positive” which means consumers are well-receptive to broiler carcass in traditional markets with most attributes considered in the purchase decision of chicken carcasses in traditional markets of Samarinda City is the color of broiler carcasses. The order of attributes from the most considered to the least considered is color, carcass weight, aroma, skin hygiene and broiler carcass price. Based on Customer Satisfaction Index of consumer satisfaction level against broiler carcass is 77.11% who are satisfied category.
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Branciari, Raffaella, Andrea Onofri, Fausto Cambiotti, and David Ranucci. "Effects of Animal, Climatic, Hunting and Handling Conditions on the Hygienic Characteristics of Hunted Roe Doer (Caprelous capreolus L.)." Foods 9, no. 8 (August 7, 2020): 1076. http://dx.doi.org/10.3390/foods9081076.
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The population of wild animals is increasing, and control strategies based on selective hunting are among the major options adopted. The game meat obtained is therefore available for controlled and certified valuable chains. The understanding of carcass contamination and the factors affecting it is therefore crucial to ensure meat safety and prolonged shelf-life. The carcass hygiene of 64 hunted wild male roe deer (Capreolus capreolus L.) was evaluated in relation to factors potentially affecting it. Aerobic colony and Enterobacteriaceae counts, as well as Salmonella spp. and Listeria monocytogenes detection, were performed. The interaction of the microbial determination with age and weight of the animals, the climate conditions, the shooting procedure, the time between the killing and the evisceration as well as the time of storage of the carcasses in refrigerated conditions before skinning, were evaluated. Neither Salmonella spp. nor Listeria monocytogenes were detected on the carcasses and the average loads detected were 3.39 ± 1.06 UFC/cm2 and 2.27± 1.11 UFC/cm2 for the aerobic colony count and Enterobacteriaceae count, respectively. The loads detected are similar to those reported by UE legislation for slaughtered species. The time of storage before skinning, the environmental temperature during hunting and the time between shooting and evisceration, associated with animal weight, affect the carcass hygiene and must be taken into careful consideration by hunters as food business operators.
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PURNELL, GRAHAM, CHRISTIAN JAMES, CAROL-ANN WILKIN, and STEPHEN J. JAMES. "An Evaluation of Improvements in Carcass Hygiene through the Use of Anal Plugging of Pig Carcasses Prior to Scalding and Dehairing." Journal of Food Protection 73, no. 6 (June 1, 2010): 1108–10. http://dx.doi.org/10.4315/0362-028x-73.6.1108.
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This study evaluated the effect of plugging the anuses of pork carcasses prior to scalding and dehairing on Enterobacteriaceae contamination on pork carcass surfaces. Results showed that Enterobacteriaceae counts around the anuses of unplugged carcasses increased by 1.15 log CFU/cm2 after the scalding and dehairing process, while counts in the same sample area from plugged carcasses showed no significant change in Enterobacteriaceae numbers. The percentage of plugged carcasses with Enterobacteriaceae counts (measured around the anuses of carcasses) below the level of detection (<1 log) was 23.5%, whereas only 2.9% of unplugged carcasses were below this level. In the context of a multiple-hurdle approach where incremental reductions in microbiological counts may be seen as positive, anal plugging may have particular benefit.
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Aynewa, Derebew, Zemichael Gizaw, and Aklilu Feleke Haile. "Assessment of Bacteriological Quality of Sheep Carcasses, Effect Level of 2.5% Citric Acid Spray on Bacterial Contamination of Meat, and Hygiene Practices of Workers in a Selected Abattoir in Debrezeit Town, Central Ethiopia." Environmental Health Insights 15 (January 2021): 117863022110375. http://dx.doi.org/10.1177/11786302211037555.
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Background: Meat safety is important for public health. As part of the meat chain abattoirs are required to give attention to meat hygiene and safety in order to minimize hazards. Therefore, the current study was conducted to evaluate the bacteriological quality of sheep carcasses, knowledge and hygienic practices of workers in a selected abattoir and to determine the effect level of 2.5% citric acid spray on total coliforms and aerobic bacteria load of raw sheep carcasses surfaces. Methods: A cross-sectional study design with structured questionnaire and observational checklists observation were used. A systematic random sampling technique was employed. A total of 50 sample swabs (25 swabs before citric acid spray and 25 after citric acid spray) were randomly taken from brisket, flank and rump of sheep’s carcasses. Swabs were moistened with buffered peptone water (BPW) and samples were taken by rubbing 100 cm2 (10 cm × 10 cm) area delineated by sterile aluminum template. In addition, we administered a structured questionnaire and an observational checklists to assess knowledge and hygienic practices of workers. Bacteriological quality of sheep carcasses were analyzed using the methods described by the US bacteriological analytical manual. Results: The mean count for aerobic bacteria of the sheep carcasses before and after citric acid spray were 7.2log10 CFU/ml and 6.4log10 CFU/ml, respectively. The test results also showed that 21 (84%) and 15 (60%) of the swab samples were positive before and after spraying citric acid, respectively. The mean counts for coliform bacterial of the sheep carcasses before and after citric acid spray were 3.5log10 CFU/ml and 2.9log10 CFU/ml, respectively. The mean total aerobic and coliform counts before and after citric acid spray were significantly different ( P < .05). Regarding the hygiene condition of workers, all the respondents reported that they always washed their hands with soap before and after entering the slaughtering room and 23 (53.5%) of the workers reported that they used hot water. Thirty-one (72.1%) of the workers reported that they do not used soap to wash hands after visiting toilet. Thirty-five (81.4%) of the production workers did not wear mouth mask while handling and distribute meat/carcass. On the other hand, all of the workers wore capes, gowns and boots at the time of the observation and only 18 (18.6%) of the production workers wore gloves at the time of the survey. Conclusion: The current study revealed that significant proportion of sheep carcasses were positive for total aerobic bacteria and total coliform. Moreover, the study also showed that spraying of sheep carcasses with 2.5% citric acid significantly reduced the total coliform and aerobic counts. However, we did not assessed how much spray results to this effect. Therefore, we recommended further studies to determine how much spray of 2.5% citric acid significantly reduce bacterial contamination of sheep carcasses. In addition, the abattoir has to follow the food hazard analysis critical control point (HACCP) system to minimize meat contamination during harvesting and processing. The abattoir has to also implement strict operation laws to improve hygiene conditions of the workers. In addition, the abattoir can minimize meat contamination using 2.5% citric acid as a decontaminant.
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HORCHNER, P., L. HUYNH, J. SUMNER, P. B. VANDERLINDE, and I. JENSON. "Performance Metrics for Slaughter and Dressing Hygiene at Australian Beef Export Establishments." Journal of Food Protection 83, no. 6 (February 7, 2020): 996–1001. http://dx.doi.org/10.4315/jfp-19-591.
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ABSTRACT A study was undertaken to examine hygienic control of the slaughter and dressing process for beef cattle at Australian export processing establishments. Samples were collected from two points during the process: immediately after hide removal and at the completion of dressing before the commencement of chilling. Hindquarter and forequarter samples were collected from 24 establishments, half of which (n = 12) used some form of microbial intervention (in addition to trimming). The overall contamination level on carcass sides was low and was reduced between hide removal and entering the chiller. The concentration and prevalence of indicator bacteria were higher on samples from hindquarters than on samples from forequarters. Application of an intervention, such as hot water, in addition to trimming resulted in a greater reduction in the concentration and prevalence of indicator bacteria than trimming alone, although the level of Escherichia coli and coliform bacteria on all samples was too low to allow meaningful comparisons to be made. Salmonellae were isolated from 2.09 and 0.56% of samples after hide removal and before chilling, respectively. Application of an intervention in addition to trimming did not result in a significant reduction (P = 0.4) of Salmonella prevalence on prechill carcasses. Low levels of bacteria were found on carcasses after hide removal. This, combined with small reductions as a result of trimming and sometimes other interventions, resulted in carcasses with very low levels of bacterial contamination. If performance metrics were to be applied to the slaughter and dressing process, a measure of the expected contamination at the end of the process would provide a more unequivocal measure of the process than either contamination on the carcass after hide removal or any reduction achieved as a result of the dressing process. HIGHLIGHTS
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Brichta-Harhay, Dayna M., Michael N. Guerini, Terrance M. Arthur, Joseph M. Bosilevac, Norasak Kalchayanand, Steven D. Shackelford, Tommy L. Wheeler, and Mohammad Koohmaraie. "Salmonella and Escherichia coli O157:H7 Contamination on Hides and Carcasses of Cull Cattle Presented for Slaughter in the United States: an Evaluation of Prevalence and Bacterial Loads by Immunomagnetic Separation and Direct Plating Methods." Applied and Environmental Microbiology 74, no. 20 (August 22, 2008): 6289–97. http://dx.doi.org/10.1128/aem.00700-08.
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ABSTRACT The hide and carcass hygiene of cull cattle at slaughter in four geographically distant regions of the United States was examined from July 2005 to April 2006 by measuring the aerobic plate counts (APC) and the prevalences and loads of Salmonella and Escherichia coli O157:H7. The geometric mean log10 APC CFU/100 cm2 levels on hides and preevisceration and postintervention carcasses ranged from 6.17 to 8.19, 4.24 to 6.47, and 1.46 to 1.96, respectively, and were highest in the summer (P < 0.0001). The average prevalences of Salmonella on hides and preevisceration and postintervention carcasses were 89.6% (95% confidence interval [CI], 85.1 to 94.0), 50.2% (95% CI, 40.9 to 59.5), and 0.8% (95% CI, 0.18 to 1.42), respectively. The prevalences of E. coli O157:H7 were 46.9% (95% CI, 37.3 to 56.6) and 16.7% (95% CI, 9.8 to 23.6) on hides and preevisceration carcasses, respectively. Examination of the concomitant incidence of Salmonella and E. coli O157:H7 showed that, on average, 33.3% (95% CI, 15.9 to 69.8) of cattle hide and 4.1% (95% CI, 0.98 to 17.3) of preevisceration carcass samples were contaminated with both pathogens. The pathogen prevalence on hides and carcasses was not significantly affected by the season; however, significant differences were observed between plants with respect to the incoming pathogen load and the ability to mitigate hide-to-carcass transfer. In spite of these differences, postintervention carcass contamination was significantly reduced (P < 0.001), likely as a result of the use of one or more of the processing interventions employed at each of the four processing plants examined.
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Ombeni, Justin Birhashwirwa, Theophiline Babe, Theodore Mushambanyi Munyuli, Theophile Mitima Kashosi, and Tresor Bahihima Mwangi. "The HYGIENIC QUALITY ASSESSMENT OF FRESH BEEF MEAT IN BUKAVU URBAN SLAUGHTERHOUSES, SOUTH KIVU PROVINCE OF THE LONG SALE CHAIN: POTENTIAL HEALTH RISKS FOR CONSUMERS EASTERN D.R. CONGO." Bacterial Empire 1, no. 1 (June 1, 2018): 1. http://dx.doi.org/10.36547/be.2018.1.1.1-9.
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Meat is generally subject to multiple sources of microbial contamination related to the length and complexity of their journey from farm to consumer's table. The purpose of this study is to assess the current hygienic quality level of fresh beef slaughtered in Bukavu urban slaughterhouses, South Kivu to identify the health dangers to consumers. The meat samples were taken from 78 carcasses into three periods: at the slaughterhouse, to the market at the end of the transport position of sale and butchery. Microorganisms were sought following appropriate ISO standards. Total Aerobic Mesophilic Flora (FAMT), total coliforms, staphylococcus and other enterobacteria were counted more (p<0.001) at the slaughterhouse and the market at the end of transportation to butchery Chi²: 64.90; 82.91 and 176.5, respectively; p<0.001). Hygienic quality of beef meat is poor, this study revealed a very high level of contamination of the collar and shoulder of beef carcass analyzed from slaughterhouse to distribution location (p<0.001). The very high bacterial load of these products is observed at the slaughterhouse and the public market during carcasses transport, the lesser butchery. This charge varies as well according to the slaughterhouse where the sampling took place, site and date of collection, including public slaughterhouse, most visited by distributors and that of suburban is the most famous. Beef carcasses are contaminated almost at the end of the week (thursday) by pathogenic bacteria such as; Staphylococcus aureus, Salmonella ssp., E. coli, coliforms and other Enterobacteriaceae represent a great danger of food poisoning to consumers, hence the need to implement an effective program against beef contamination, veal and respect for hygiene breeding farm, slaughterhouses, slaughter procedures, method of handling meat, and transport to the sale to consumer.
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LUCIANEZ, A., M. A. HOLMES, and A. W. TUCKER. "Relationships between Processing Delay and Microbial Load of Broiler Neck Skin Samples." Journal of Food Protection 73, no. 1 (January 1, 2010): 26–31. http://dx.doi.org/10.4315/0362-028x-73.1.26.
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The measurable microbial load on poultry carcasses during processing is determined by a number of factors including farm or origin, processing hygiene, and external temperature. This study investigated associations between carcass microbial load and progressive delays to processing. A total of 30 carcasses were delayed immediately after defeathering and before evisceration in a commercial abattoir in groups of five, and were held at ambient temperature for 1, 2, 3, 4, 6, and 8 h. Delayed carcasses were reintroduced to the processing line, and quantitative assessment of total viable count, coliforms, Staphylococcus aureus, and Pseudomonas spp. was undertaken on neck skin flap samples collected after carcass chilling and then pooled for each group. Sampling was repeated on 5 separate days, and the data were combined. Significant increases in total viable count (P = 0.001) and coliforms (P = 0.004), but not for S. aureus or Pseudomonas loads, were observed across the 8-h period of delay. In line with previous studies, there was significant variation in microbiological data according to sampling day. In conclusion, there is a significant and measurable decline in microbiological status of uneviscerated but defeathered poultry carcasses after an 8-h delay, but the variability of sampling results, reflecting the wide range of factors that impact microbial load, means that it is not possible to determine maximum or minimum acceptable periods of processing delay based on this criterion alone.
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YEH, KUANG-SHENG, SHIH-PING CHEN, and JIUNN-HORNG LIN. "One-Year (2003) Nationwide Pork Carcass Microbiological Baseline Data Survey in Taiwan." Journal of Food Protection 68, no. 3 (March 1, 2005): 458–61. http://dx.doi.org/10.4315/0362-028x-68.3.458.
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From January through December 2003, swab samples from 1,650 pork carcasses were collected from 39 slaughter plants in Taiwan. These samples were analyzed for the prevalence of indicator microorganisms and specific pathogens. Viable aerobic bacteria, total coliforms, and Escherichia coli were recovered from 100, 95.3, and 87.5% of these carcasses, respectively. Of those carcasses that harbored bacteria, the mean aerobic plate, total coliform, and Escherichia coli counts were 4.0, 0.6, and 0.1 log CFU/cm2, respectively. Staphylococcus aureus, Clostridium perfringens, Campylobacter jejuni, Campylobacter coli, Listeria monocytogenes, and Salmonella were recovered from 4.8, 0.3, 13.8, 0.7, and 1.7 of 1,038 carcasses, respectively. E. coli O157:H7 was not detected from any carcass. When positive for a specific pathogen, the mean carcass concentration was 0.57 log CFU/cm2 for S. aureus, 0.66 most probable number (MPN)/cm2 for C. jejuni and C. coli, and 0.18 MPN/cm2 for Salmonella. The findings of this study will help provide a reference for establishing hygienic standards and a criterion for evaluating the effects of slaughtering operations in Taiwan.
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Lloyd, Joan, Johann Schröder, and David Rutley. "Trimming and production losses associated with bacterial arthritis in lambs presented to an abattoir in southern Australia." Animal Production Science 59, no. 5 (2019): 933. http://dx.doi.org/10.1071/an17427.
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Bacterial arthritis in sheep is a painful and debilitating condition that is widespread across all sheep-raising regions and climatic zones of Australia. At slaughter, bacterial arthritis in sheep causes economic losses through the condemnation of all or parts of the carcass, as specified in the Australian Standard for the Hygienic Production and Transportation of Meat and Meat Products for Human Consumption. In this study, we investigated the economic cost of arthritis in lambs presenting to an abattoir in southern Australia using a combination of the prevalence of arthritis detected during meat inspection, condemnation rates, trim weight and carcass weight, and fat measurements. Data were collected on 354 lines of lambs representing 63287 carcasses. One hundred and sixty nine consignments, or approximately one-half of the consignments, had at least one carcass with arthritis/polyarthritis detected by meat inspection personnel. Four hundred and twenty-two, or 0.7%, of the carcasses had arthritis/polyarthritis in at least one joint. When arthritis was present, on average 2.0% of the line was affected. Three carcasses with arthritis were condemned and the remainder trimmed, with an average trim weight of 0.7 kg. In addition, arthritis reduced the growth of lambs by 1.2 kg hot standard carcass weight, approximately 2.7 kg liveweight (assuming 45% dressing percentage) and reduced fat cover by 1.8 mm.
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RUBY, JOHN R., and STEVEN C. INGHAM. "Use of Enterobacteriaceae Analysis Results for Predicting Absence of Salmonella Serovars on Beef Carcasses." Journal of Food Protection 72, no. 2 (February 1, 2009): 260–66. http://dx.doi.org/10.4315/0362-028x-72.2.260.
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Previous work using a large data set (no. 1, n = 5,355) of carcass sponge samples from three large-volume beef abattoirs highlighted the potential use of binary (present or absent) Enterobacteriaceae results for predicting the absence of Salmonella on carcasses. Specifically, the absence of Enterobacteriaceae was associated with the absence of Salmonella. We tested the accuracy of this predictive approach by using another large data set (no. 2, n = 2,163 carcasses sampled before or after interventions) from the same three data set no. 1 abattoirs over a later 7-month period. Similarly, the predictive approach was tested on smaller subsets from data set no. 2 (n = 1,087, and n = 405) and on a much smaller data set (no. 3, n = 100 postintervention carcasses) collected at a small-volume abattoir over 4 months. Of Enterobacteriaceae-negative data set no. 2 carcasses, >98% were Salmonella negative. Similarly accurate predictions were obtained in the two data subsets obtained from data set no. 2 and in data set no. 3. Of final postintervention carcass samples in data set nos. 2 and 3, 9 and 70%, respectively, were Enterobacteriaceae positive; mean Enterobacteriaceae values for the two data sets were −0.375, and 0.169 log CFU/100 cm2 (detection limit = −0.204, and Enterobacteriaceae negative assigned a value of −0.505 log CFU/100 cm2). Salmonella contamination rates for final postintervention beef carcasses in data set nos. 2 and 3 were 1.1 and 7.0%, respectively. Binary Enterobacteriaceae results may be useful in evaluating beef abattoir hygiene and intervention treatment efficacy.
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Bakkar, M. N., S. M. Basmaeil, and A. A. Hamam. "Meat production and quality two unique properties of young camels." Revue d’élevage et de médecine vétérinaire des pays tropicaux 53, no. 2 (February 1, 2000): 218. http://dx.doi.org/10.19182/remvt.9756.
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(texte complet en anglais)Eighteen one-hump black (Majaheem) young camels were used to study growth rate and carcass percentage. The animals were selected and bought of similar age (about six months) and weight (around 150 kg). They were assigned randomly into three feeding groups. Each group was fed two types of rations: (A) concentrated pellets 18% crude protein at the rate of 1.5% CWT; and (B) one of three types of roughage: (a) alfalfa hay, (b) Rhodes grass hay, or (c) wheat straw treated with ammonia gas. Roughage was weighed and fed ad libitum. The experiment was ended when anirnals were about 14 months of age. Average body weights were 315, 298.8 and 291.4 kg for the three groups, respectively. Average daily gains for the three respective groups were 932, 803 and 767 g per day. Average carcass weights/kg (and carcass percentages) of each group were 180.6 (57.3%) for alfalfa, 170.7 (57.1%) for Rhodes and 168.1 (57.7%) for treated straw. Average forequarters weights/kg (and percentages to the carcass) were 98.9 (54.8%), 95.6 (56%) and 88.1 (52.4%) for the three groups, respectively. The hindquarters average weights/kg (and percentages) for the three respective groups were: 81,7 (45.2%), 75.1 (44%) and 80 (47.6%). Eight-year records (1986-1994) on carcasses of carnels, cattle and sheep were obtained from Riyadh Modem Slaughterhouse. The total number of animals killed was 3,891,301. Comparisons were made between the number of anirnals killed and the number of carcasses destroyed for each of the three species. It was found that on average for every carnel carcass destroyed 12 carcasses of cattle and 19 carcasses of sheep were destroyed. These results show clearly that camels produce the most hygienic meat for human consumption in comparison with cattle and sheep.
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GHAFIR, Y., B. CHINA, K. DIERICK, L. DE ZUTTER, and G. DAUBE. "Hygiene Indicator Microorganisms for Selected Pathogens on Beef, Pork, and Poultry Meats in Belgium." Journal of Food Protection 71, no. 1 (January 1, 2008): 35–45. http://dx.doi.org/10.4315/0362-028x-71.1.35.
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Several bacterial indicators are used to evaluate hygiene during the meat slaughtering process. The objectives of this study were to assess the Belgian baseline data on hygienic indicators and the relationship between the indicators and zoonotic agents to establish hygiene indicator criteria for cattle, pig, and chicken carcasses and meat. The study used the results from the official Belgian surveillance plan from 2000 to 2003, which included the monitoring of Escherichia coli counts (ECC), Enterobacteriaceae counts (EC), aerobic colony counts (ACC), and Pseudomonas counts (PC). The sampling method was the wet and dry swabbing technique for cattle and pig carcasses and neck skin excision for broiler and layer chicken carcasses. The 75th and 95th percentiles of ECC were −0.20 and 0.95 log CFU/cm2 for cattle carcasses, 1.20 and 2.32 log CFU/cm2 for pig carcasses, and 4.05 and 5.24 log CFU/g for chicken carcasses. The ACC were 2.1- to 4.5-log higher than the ECC for cattle, pigs, and chickens. For cattle and pig carcasses, a significant correlation between ECC, EC, and ACC was found. ECC for pork and beef samples and EC in pig carcasses were significantly higher in samples contaminated with Salmonella. In poultry samples, ECC were in general higher for samples containing Salmonella or Campylobacter. Thus, E. coli may be considered as a good indicator for enteric zoonotic agents such as Salmonella for beef, pork, and poultry samples and for Campylobacter in poultry samples.
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Roila, Rossana, Caterina Altissimi, Raffaella Branciari, Sara Primavilla, Andrea Valiani, Fausto Cambiotti, Lorenzo Cardinali, Attilia Cioffi, and David Ranucci. "Effects of Spray Application of Lactic Acid Solution and Aromatic Vinegar on the Microbial Loads of Wild Boar Carcasses Obtained under Optimal Harvest Conditions." Applied Sciences 12, no. 20 (October 15, 2022): 10419. http://dx.doi.org/10.3390/app122010419.
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Solutions of lactic acid 2% and aromatic vinegar were investigated for microbial load reduction on the surfaces of wild boar carcasses. The animals were hunted and processed according to production specifications to obtain the best hygiene for carcasses. The solutions were sprayed on carcass surfaces after skinning, and sites of 5 × 5 cm were sampled 2 h and 48 h post-treatment, with the carcasses under refrigeration conditions. The values of the microbial loads were lower for lactic-acid-treated sites, compared with control, after both 2 and 48 h. Nonetheless, the differences in the microbial loads were only higher than 1 Log/CFU 25 cm2 for the aerobic colony count. The aromatic vinegar resulted in lower values than in controls only after 48 h for the aerobic colony count, Staphylococcus count, and Lactobacillus count, with values always below 1 Log/CFU 25 cm2. The implementation of a lactic acid solution could represent a valuable strategy to reduce microbial growth on wild boar carcasses, thus becoming a relevant critical control point in this peculiar and niche meat production process.
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Jaros, P., A. L. Cookson, A. Reynolds, H. Withers, R. Clemens, G. Brightwell, J. Mills, et al. "The effect of transportation and lairage on faecal shedding and carcass contamination with Escherichia coli O157 and O26 in very young calves in New Zealand." Epidemiology and Infection 146, no. 9 (May 23, 2018): 1089–100. http://dx.doi.org/10.1017/s0950268818000973.
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AbstractThe effect of transportation and lairage on the faecal shedding and post-slaughter contamination of carcasses with Escherichia coli O157 and O26 in young calves (4–7-day-old) was assessed in a cohort study at a regional calf-processing plant in the North Island of New Zealand, following 60 calves as cohorts from six dairy farms to slaughter. Multiple samples from each animal at pre-slaughter (recto-anal mucosal swab) and carcass at post-slaughter (sponge swab) were collected and screened using real-time PCR and culture isolation methods for the presence of E. coli O157 and O26 (Shiga toxin-producing E. coli (STEC) and non-STEC). Genotype analysis of E. coli O157 and O26 isolates provided little evidence of faecal–oral transmission of infection between calves during transportation and lairage. Increased cross-contamination of hides and carcasses with E. coli O157 and O26 between co-transported calves was confirmed at pre-hide removal and post-evisceration stages but not at pre-boning (at the end of dressing prior to chilling), indicating that good hygiene practices and application of an approved intervention effectively controlled carcass contamination. This study was the first of its kind to assess the impact of transportation and lairage on the faecal carriage and post-harvest contamination of carcasses with E. coli O157 and O26 in very young calves.
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GILL, C. O., M. BADONI, and T. JONES. "Hygienic Effects of Trimming and Washing Operations in a Beef-Carcass-Dressing Process†." Journal of Food Protection 59, no. 6 (June 1, 1996): 666–69. http://dx.doi.org/10.4315/0362-028x-59.6.666.
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Swab samples were obtained from the surfaces of randomly selected beef carcasses passing through a high-speed dressing process. A single sample was obtained from a randomly selected site on the surface of each selected carcass. Fifty such samples were collected at each of four stages in the process. The aerobic bacteria, coliforms, and Escherichia coli recovered from each sample were enumerated. Values for the mean log units and standard deviations of each set of 50 log values were calculated on the assumption that the log values were normally distributed. The log of the arithmetic mean was estimated from the mean log and standard deviation values for each set. The results show that the average numbers of E. coli, coliforms, and aerobic bacteria which are deposited on carcasses during skinning and evisceration are not reduced by trimming, and that washing approximately halves the average numbers of those bacteria on carcasses. It is concluded that commercial trimming and washing operations are not effective means of decontaminating beef carcasses.
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CAPITA, ROSA, MIGUEL PRIETO, and CARLOS ALONSO-CALLEJA. "Sampling Methods for Microbiological Analysis of Red Meat and Poultry Carcasses." Journal of Food Protection 67, no. 6 (June 1, 2004): 1303–8. http://dx.doi.org/10.4315/0362-028x-67.6.1303.
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Microbiological analysis of carcasses at slaughterhouses is required in the European Union for evaluating the hygienic performance of carcass production processes as required for effective hazard analysis critical control point implementation. The European Union microbial performance standards refer exclusively to the excision method, even though swabbing using the wet/dry technique is also permitted when correlation between both destructive and nondestructive methods can be established. For practical and economic reasons, the swab technique is the most extensively used carcass surface-sampling method. The main characteristics, advantages, and limitations of the common excision and swabbing methods are described here.
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Kalio, G. A., and A. Ali-Uchechukwu. "Assessment of abattoirs operations and hygiene practices in Obio-Akpor Local Government Area, Rivers State, Nigeria." Nigerian Journal of Animal Production 46, no. 3 (December 23, 2020): 73–81. http://dx.doi.org/10.51791/njap.v46i3.858.
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A survey was conducted to appraise the location, infrastructures, and slaughter operational activities in three abattoirs at Rumuokoro, Trans-Amadi and Rumuosi in Obio-Akpor Local Government Area of Rivers State, Nigeria. Thirty butchers of 10 per abattoir were purposively sampled for the study. The instrument used for data collection was a structured questionnaire. Data were analyzed using frequencies, percentages and mean statistics Results of the socio-demographic characteristics of the butchers revealed that they were all males, married, within the age of 35 - 44years, literate, with a butchering experience of 16 years upwards in private or public/Government owned abattoirs. The slaughter houses were located in areas devoid of bad odours, smoke, dust and close to coastal areas affected by tides to promote good sanitation. There were no adequately constructed lairages, Ante-mortem inspection and isolation pens were inadequate. There were no facilities and rooms for handling hides and skins, treatment of inedible and condemned carcass and meat. Animals were poorly restrained and not stunned before slaughtering. The slaughter houses had planned and controlled cleaning programs but lacked efficient disinfection operations and this will contribute to bacterial contamination of carcasses. The operational lapses by butchers were due to the use of obsolete facilities that lacked maintenance and inadequate supervision. This condition had brought about poor hygienic practices that undermine the quality of carcasses. The recommendations proffered are: the provision and maintenance facilities for effective operations to ensure carcass quality, to adequately enforce enacted laws on abattoir operations, there should be enlightenment for butchers and meat sellers, and there should be effective management of the wastes from slaughterhouses through regular fumigations and disposal systems.
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Tsitsos, Anestis, Vangelis Economou, Eirini Chouliara, Ioannis Ambrosiadis, and Georgios Arsenos. "A Comparative Study on Microbiological and Chemical Characteristics of Small Ruminant Carcasses from Abattoirs in Greece." Foods 11, no. 15 (August 7, 2022): 2370. http://dx.doi.org/10.3390/foods11152370.
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Meat quality dictates consumer preferences with hygiene forming a key component, especially in meat types with declining popularity, such as sheep and goat meat. Aiming to increase the marketability of sheep and goat meat, we examined 370 sheep and goat carcasses from two abattoirs in Greece. Tests included enumeration of the total mesophilic viable count, total psychrophilic viable count and coliform count, and detection of Salmonella spp., Listeria monocytogenes and presumptive ESBL Escherichia coli. Moreover, designated samples of meat were used to measure pH, moisture, total fat and protein content. Goat carcasses had significantly higher microbial counts compared to sheep carcasses. Lamb and kid carcasses had larger TMVC, TPVC and coliform counts compared to carcasses from adult animals. One strain of L. monocytogenes (0.8%), typed as serovar 1/2a (3a), was isolated from one adult sheep carcass. Twelve strains of ESBL Escherichia coli (25%) were isolated; there were not any strains of Salmonella spp. The average values of pH, moisture, total fat and total protein were 5.83%, 67.76%, 7.21% and 21.31%, respectively, for sheep carcasses and 5.70%, 68.2%, 5.69% and 24.10%, respectively, for goat carcasses. The results showed a small deviation in assessed parameters, implying the uniformity of the conditions concerning rearing and slaughtering.
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GILL, C. O., J. C. MCGINNIS, and M. BADONI. "Assessment of the Hygienic Characteristics of a Beef Carcass Dressing Process." Journal of Food Protection 59, no. 2 (February 1, 1996): 136–40. http://dx.doi.org/10.4315/0362-028x-59.2.136.
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Swab samples were obtained from the surfaces of randomly selected beef carcasses passing through a high-speed dressing process. A single sample was obtained from each selected carcass from one of 10 sites. At each of 3 points in the process, 25 samples were obtained from each carcass site. The aerobic bacteria, coliforms, and Escherichia coli recovered from each sample were enumerated. Values for the means and standard deviations of each set of 25 values were calculated on the assumption that each set of values was log-normally distributed. The E. coli data indicated relatively heavy (mean log numbers > 2/100 cm2) contamination of carcasses with E. coli during the skinning of posterior sites; redistribution of E. coli, from relatively heavily to relatively lightly (mean log numbers about 0/100 cm2) contaminated sites during evisceration operations, and reduction of E. coli numbers at most sites as a result of trimming and washing operations. However, posterior sites remained the most heavily contaminated with E. coli. The findings for coliforms were similar to those for E. coli. In contrast, the total count data indicated heavy (mean log numbers > 3/cm2) contamination of anterior (brisket) sites as well as posterior sites and little redistribution of bacteria during evisceration operations. After trimming and washing operations, the mean log total numbers at most sites were about 2/cm2, but one brisket site remained heavily contaminated. It is suggested that E. coli or coliform data are appropriate for assessing carcass dressing processes for hazard analysis critical control point (HACCP) system purposes, while total count data are inappropriate for that purpose but may be appropriate, in relation to product storage stability, for quality management (QM) system purposes.
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NWANKWO, Innocent O., Ekene V. EZENDUKA, John A. NWANTA, Akwoba J. OGUGUA, and Benshak J. AUDU. "Prevalence of Campylobacter spp. and antibiotics resistant E. coli on poultry carcasses and handlers’ hands at Ikpa slaughter, Nsukka, Nigeria." Notulae Scientia Biologicae 13, no. 2 (June 2, 2021): 10866. http://dx.doi.org/10.15835/nsb13210866.
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Handling and consumption of contaminated meat can lead to food poisoning and acquisition of antibiotics resistance genes. This study determined the prevalence of Campylobacter spp. and antibiotics resistant E. coli on poultry carcasses and handler’s hands at Ikpa slaughter, Nsukka. Swabs collected from carcass surfaces and handler’s hands were cultured and isolates were identified with phenotypic and molecular methods. Coliform enumeration, antibiotics susceptibility testing and data analysis were done. Out of 204 samples, 29 (14.2%) were Campylobacter positive, consisting of C. jejuni (27.6%) and C. coli (72.4%) while another 67 samples yielded 23 (34.4%) positive E. coli. Coliform counts were 3.2x103cfu cm-2 and 1.06x103cfu cm-2for unwashed and washed carcasses, 2.5x105 cfu cm-2 and 0.5x104 cfu cm-2 for unwashed and washed handlers’ hands respectively. E. coli was isolated from 45 and 22% of unwashed and washed carcasses respectively and Campylobacter from 16% of unwashed carcasses only. One and five sticks swabbing methods yielded E. coli in 20.5 and 60.9% and Campylobacter in 12 and 83.3% of carcass samples respectively. Only E. coli was detected in 10 (90.0%) of the unwashed hands. E. coli isolates were multi drug-resistant at 80% with index >0.2 and a range of 70 -100% resistant to enrofloxacin, tetracycline, erythromycin, streptomycin and ampicillin; 30 - 50% to gentamycin, chloramphenicol and ciprofloxacin while; 10% were to cephtriazone and amoxicillin/clavulanic acid. Prevalence of Campylobacter and antibiotics resistant E. coli spp. were significantly associated (P<0.05) with status of processor’s hands and swabbing methods hence, the public health risk and the need for improved hygiene.
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ARGUELLO, HECTOR, AVELINO ÁLVAREZ-ORDOÑEZ, ANA CARVAJAL, PEDRO RUBIO, and MIGUEL PRIETO. "Role of Slaughtering in Salmonella Spreading and Control in Pork Production." Journal of Food Protection 76, no. 5 (May 1, 2013): 899–911. http://dx.doi.org/10.4315/0362-028x.jfp-12-404.
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Salmonella is one of the major foodborne pathogens worldwide. Pork products are among the main sources of Salmonella infection in humans, and several countries have established Salmonella surveillance and control programs. The role of slaughtering in carcass contamination has been indicated by studies focused on the slaughterhouse environment. In this review, we examine and discuss the information available regarding the influence that farm status, pig transport, and lairage have on the carriage of Salmonella by pigs entering the slaughter line. The evolution of carcass contamination throughout the slaughtering process, the main sources of contamination in the dirty and clean zones of the slaughter line, and previously reported prevalence of Salmonella on carcasses and factors affecting this prevalence also are discussed. The importance of implementing interventions at the slaughter level is discussed briefly. Consistent with the information available, pigs from infected farms and newly acquired or recrudescent infections in pigs at the subsequent stages of transport and lairage are important sources of Salmonella at the slaughtering plant. The continuous introduction of Salmonella into the slaughterhouse and the potential for resident flora constitute a risk for carcass contamination. At the slaughterhouse, some dressing activities can reduce carcass contamination, but others are critical control points that jeopardize carcass hygiene. This information indicates the importance of considering slaughter and previous stages in the pork production chain for controlling Salmonella in swine production.
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GIOMBELLI, AUDECIR, and MARIA BEATRIZ ABREU GLORIA. "Prevalence of Salmonella and Campylobacter on Broiler Chickens from Farm to Slaughter and Efficiency of Methods To Remove Visible Fecal Contamination." Journal of Food Protection 77, no. 11 (November 1, 2014): 1851–59. http://dx.doi.org/10.4315/0362-028x.jfp-14-200.
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A study was conducted to investigate the prevalence of Salmonella and Campylobacter from farm to slaughter. The efficiency of trimming and water spray (490 to 588 kPa pressure) on the removal of visible fecal contamination from broiler carcasses before chilling was also investigated. Drag swabs were used to sample litter from the farm houses. Samples of ceca and carcasses without and with visible fecal contamination before and after trimming or spray washing of fecal contamination were taken during slaughter of the flocks previously visited at the farms. There was a low prevalence of Salmonella on the litter from the farms (5%) and cecum and carcasses (0%). However, Campylobacter jejuni and Campylobacter coli were present in farms' litter (100 and 58.8%, respectively), cecum samples (100 and 70.6%, respectively), and carcasses with (58.8 and 11.6%, respectively) and without (17.6 and 9.8%, respectively) visible fecal contamination. There was high prevalence of C. jejuni but at low counts and low prevalence and high counts of C. coli. Campylobacter lari was not detected in any sample. Trimming the visible fecal contamination decreased the prevalence of C. jejuni but increased occurrence of C. coli. Trimming did not reduce the counts of Campylobacter and of hygiene indicator microorganisms on the carcasses. Water shower reduced the counts of hygiene indicator microorganisms by 20%. Therefore, control measures for preventing introduction of Campylobacter and the use of good hygienic conditions are needed to warrant the microbiological quality and safety of broiler carcasses.
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JERICHO, K. W. F., G. O'LANEY, and G. C. KOZUB. "Verification of the Hygienic Adequacy of Beef Carcass Cooling Processes by Microbiological Culture and the Temperature-Function Integration Technique." Journal of Food Protection 61, no. 10 (October 1, 1998): 1347–51. http://dx.doi.org/10.4315/0362-028x-61.10.1347.
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To enhance food safety and keeping quality, beef carcasses are cooled immediately after leaving the slaughter floor. Within hazard analysis and critical control point (HACCP) systems, this cooling process needs to be monitored by the industry and verified by regulatory agencies. This study assessed the usefulness of the temperature-function integration technique (TFIT) for the verification of the hygienic adequacy of two cooling processes for beef carcasses at one abattoir. The cooling process passes carcasses through a spray cooler for at least 17 h and a holding cooler for at least 7 h. The TFIT is faster and cheaper than culture methods. For spray cooler 1, the Escherichia coli generations predicted by TFIT for carcass surfaces (pelvic and shank sites) were compared to estimated E. coli counts from 120 surface excision samples (rump, brisket, and sacrum; 5 by 5 by 0.2 cm) before and after cooling. Counts of aerobic bacteria, coliforms, and E. coli were decreased after spray cooler 1 (P ≤ 0.001). The number of E. coli generations (with lag) at the pelvic site calculated by TFIT averaged 0.85 ± 0.19 and 0.15 ± 0.04 after emerging from spray coolers 1 and 2, respectively. The TFIT (with lag) was considered convenient and appropriate for the inspection Service to verify HACCP systems for carcass cooling processes.
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Olukemi, Adesiji Yemisi, Igbinigie Mavis Osas, Olaitan Janet Olubukola, and Ogah Ikhevha Jeremiah. "Bacterial Contamination Associated with Retail Chicken Carcasses in Osogbo, Nigeria." Journal of Health and Allied Sciences NU 05, no. 04 (December 2015): 045–50. http://dx.doi.org/10.1055/s-0040-1703934.
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Abstract Background: Worldwide, food borne illness is often associated with consumption of meat and poultry products sold at retail markets. A study on the bacteriological status of chicken carcass in Osogbo, Nigeria, was carried out to determine the prevalence of Arcobacter species, Escherichia coli and Staphylococcus aureus in chicken carcasses. Methodology: A total of 100 samples of chicken carcasses were collected from two major processing points in Osogbo, Nigeria. The samples were analysed for the presence of bacterial contaminants using standard microbiological isolation and identification procedures, with antimicrobial susceptibility test performed using the disk diffusion method. Result: Of hundred chicken carcasses sampled, 38% were positive for Arcobacter species and E. coli while 60% accounted for S. aureus isolates. Ninety percent of Arcobacter spp isolates were susceptible to ciprofloxacin, 85% to gentamicin, and pefloxacin, 70% to chloramphenicol and 90% were resistant to amoxicillin, 85% to augumentin and 80% to streptomycin. Hundred percent of E. coli isolates were susceptible to ciprofloxacin, pefloxacin, 95% to gentamicin and 100% were resistant to streptomycin, 85 % resistant to amoxicillin, augumentin, while 100% of S. aureus isolates were susceptible to trimethoprim sulphamethoxazole, 90% susceptible to gentamicin, 80% to streptomycin and 100% of the S.aureus isolates were resistant to ampliclox. Conclusion: The bacteriological status of chicken carcass revealed high contamination with Arcobacter, E coli and S. aureus with varying degree of antibiotic resistance therefore, improvement in meat processing procedures and strict hygiene measures towards reduction of these pathogens in food products should be encouraged.
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MURRAY, KATHRYN A., ARTHUR GILMOUR, and ROBERT H. MADDEN. "Microbiological Quality of Chilled Beef Carcasses in Northern Ireland: A Baseline Survey." Journal of Food Protection 64, no. 4 (April 1, 2001): 498–502. http://dx.doi.org/10.4315/0362-028x-64.4.498.
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To standardize the assessment of the hygienic quality of beef carcasses in Northern Ireland (NI) abattoirs, swabbing techniques were evaluated. Six materials, including two commercially produced swabs, were compared for their ability to recover spoilage and pathogenic bacteria and for their ease of use as carcass swabs. A sponge retailed for domestic use was selected on the basis of efficiency of recovery of microorganisms, ease of use, and cost. On sample carcasses, 1,000 cm2 of the brisket was swabbed, since this site is normally readily contaminated. For 9 months, 420 carcasses in seven of the nine European Union–approved abattoirs in NI were sampled while in the chiller (24 to 48 h after kill). Total viable count (TVC), yeasts and molds, and Enterobacteriaceae were enumerated after incubation at 22 (48 h) and 37°C (48 h), and the results were expressed as log CFU/cm2. The mean TVC results at 22 and 37°C were 2.80 ± 0.70 and 2.75 ± 0.64, respectively. Although 63% of samples had yeasts that grew at 22°C, only 35% were positive at 37°C. The respective mean yeast counts were 1.12 ± 0.59 and 0.46 ± 0.51. Enterobacteriaceae were present in 15% of samples at 22°C and 21% of samples at 37°C. The mean counts for positive samples were 0.41 ± 0.37 and 0.40 ± 0.30, respectively. Molds were found in less than 4% of samples. Given that the brisket is normally one of the most heavily contaminated parts of the carcass, these results suggest that good hygienic practices are in operation in NI abattoirs. The results also enabled the abattoirs with the cleanest carcasses to be identified, hence permitting best practices to be found.
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Cevallos-Almeida, María, Ana Burgos-Mayorga, Carlos A. Gómez, José Luis Lema-Hurtado, Leydi Lema, Iveth Calvache, Christian Jaramillo, Isabel Collaguazo Ruilova, Evelyn Pamela Martínez, and Pamela Estupiñán. "Association between animal welfare indicators and microbiological quality of beef carcasses, including Salmonella spp., from a slaughterhouse in Ecuador." Veterinary World 14, no. 4 (April 16, 2021): 918–25. http://dx.doi.org/10.14202/vetworld.2021.918-925.
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Background and Aim: Pre-slaughter management and slaughter operations are considered critical factors for animal welfare and meat quality. Previous studies have found poor animal welfare management at municipal slaughterhouses in Ecuador, and little is known about how this affects the microbiological quality of the meat. Therefore, the aim of the study was to analyze the association of the microbiological quality of beef carcasses and animal welfare indicators in a municipal slaughterhouse in Ecuador. Materials and Methods: Data for 6 months were collected from a municipal slaughterhouse in Ecuador. Five trained researchers were strategically located along the slaughter process. A total of 351 animals were observed with regard to welfare indicators, and their carcasses were sampled to evaluate microbiological quality. Antemortem (slipping, falling, and vocalization) and postmortem animal welfare indicators (bleed interval, pH, temperature, and bruises) were measured. To determine the total aerobic bacteria (TAB) and Escherichia coli counts and the presence of Salmonella spp., we collected samples by swabbing four different points of each carcass. The association between microbiological quality and animal welfare indicators was studied using univariate and multivariate logistic regressions. Results: The mean TAB count was 5.3 log CFU/cm2, and the mean total count of E. coli was 2.4 log CFU/cm2. Salmonella spp. were isolated in 3.1% of the carcasses. An electric goad was used in all animals, 19.1% slipped at least once, and 19.9% vocalized. The mean pH of the carcasses was 7.2, and 79.2% of carcasses had bruises. Multivariate analysis showed that Salmonella spp. and the TAB count were associated with pH and the number of bruises (p = 0.01 in both cases). Conclusion: Although there was non-significant association between the majority of animal welfare indicators and microbiological quality, the poor management affecting animal welfare and carcass hygiene are worrisome.
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INGHAM, STEVEN C., RYAN J. ALGINO, BARBARA H. INGHAM, and JOHN R. RUBY. "Manual Squeezing as an Alternative to Mechanical Stomaching in Preparing Beef Carcass Sponge Samples for Microbiological Analysis." Journal of Food Protection 72, no. 2 (February 1, 2009): 428–30. http://dx.doi.org/10.4315/0362-028x-72.2.428.
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The U.S. Department of Agriculture (USDA) requires beef abattoir operators to periodically analyze beef carcass sponge samples for levels of Escherichia coli. Additional beef carcass sponge sampling is commonly used by processors to evaluate the efficacy of beef abattoir antimicrobial intervention systems. The USDA sample preparation procedure requires that beef carcass sponge samples be mechanically stomached for 2 min before the sample fluid is squeezed out for analysis. When a large number of sponge samples must be analyzed, the stomaching step can limit throughput. In this study, we compared the USDA sample preparation procedure with repeatedly squeezing the sponge during a 10-s interval to expel the sample fluid. Separate sponge samples were obtained from each half of 100 chilled postintervention beef carcasses from a large-volume abattoir during a 4-month period. The USDA and squeezing treatments were randomly assigned to the halves of each carcass. All sponge samples were analyzed for E. coli, coliforms, Enterobacteriaceae, and aerobic mesophilic bacteria using Petrifilm methods. The sample preparation method had no significant effect (signed rank value > 0.05) on the results of any analytical test, although aerobic mesophilic bacteria counts tended to be higher after the USDA method than after manual squeezing alone. These results suggest that manual squeezing may be a simple and rapid alternative sample preparation method when gram-negative bacteria such as E. coli, coliforms, or Enterobacteriaceae are being enumerated from beef carcass sponge samples used to monitor operational abattoir hygiene.
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Alonso-Calleja, Carlos, Emilia Guerrero-Ramos, and Rosa Capita. "Hygienic Status Assessment of Two Lamb Slaughterhouses in Spain." Journal of Food Protection 80, no. 7 (June 9, 2017): 1152–58. http://dx.doi.org/10.4315/0362-028x.jfp-16-330.
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ABSTRACT A total of 180 lamb carcasses and 200 inert surfaces were sampled in two commercial abattoirs (plants A and B) from northwest Spain. A higher (P < 0.001) average microbial load (log CFU per square centimeter) on lamb carcasses was observed for total viable counts (TVC; 2.74 ± 1.15) than for Enterobacteriaceae (2.21 ± 1.16). Different microbial counts were found on carcasses from plants A and B, both for TVC (2.56 ± 0.96 versus 3.18 ± 1.47, respectively; P < 0.001) and Enterobacteriaceae (2.09 ± 0.97 versus 2.50 ± 1.61, respectively; P < 0.05). High correlations (P < 0.001) were observed for TVC and Enterobacteriaceae in both plants A (r = 0.708) and B (r = 0.912). The percentages of unsatisfactory daily mean log values for carcasses, according to European Union Regulation (EC) No 2073/2005, were 0.0 (TVC) and 30.8 (Enterobacteriaceae) in plant A and 10.0 (TVC) and 40.0 (Enterobacteriaceae) in plant B. Average counts for inert surfaces were all lower than 10 CFU/cm2 (TVC) or 1 CFU/cm2 (Enterobacteriaceae). The need to improve hygienic practices in order to adhere to the European Union microbiological performance criteria is emphasized. The detected different microbial counts between slaughterhouses could be attributed to differences in external hygiene of livestock and in the number of slaughterhouse workers. Microbiological analysis of carcasses and surfaces allows detection of hygienic concerns in the overall process.
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BOYSEN, LOUISE, and HANNE ROSENQUIST. "Reduction of Thermotolerant Campylobacter Species on Broiler Carcasses following Physical Decontamination at Slaughter." Journal of Food Protection 72, no. 3 (March 1, 2009): 497–502. http://dx.doi.org/10.4315/0362-028x-72.3.497.
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To reduce the incidences of human Campylobacter infections, a number of countries are investigating methods for reducing human exposure to Campylobacter from broiler meat. In addition to implementing biosecurity measures at the farm, Campylobacter may be controlled by reducing Campylobacter counts through physical decontamination of the meat. The current study was conducted to compare the Campylobacter-reducing ability of three physical decontamination techniques, forced air chilling, crust freezing, and steam-ultrasound, performed in the plant with naturally contaminated broiler chickens. The effects of all three techniques were evaluated and compared with the effect of freezing. Mean reductions obtained were 0.44 log CFU per carcass, 0.42 log CFU per sample, and ≥2.51 log CFU per carcass, respectively. All techniques resulted in significant reductions of the Campylobacter concentration on the carcasses (P < 0.05). However, none of the techniques were as effective as freezing based on reductions in Campylobacter counts and on adverse effects. The increase in Campylobacter counts on carcasses following visceral rupture during the evisceration operation also was examined. Visceral rupture resulted in an increase of 0.9 log CFU per carcass, suggesting that Campylobacter counts also may be reduced by optimizing the hygienic design of equipment or by physical removal of fecal contamination.
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Yalçın, Yasemin, Pelin Koçak Kızanlık, Cemil Şahiner, and Ergün Ömer Göksoy. "Evaluation of the effect of cooling on the microbiological quality of lamb carcasses." Food and Health 7, no. 3 (2021): 179–84. http://dx.doi.org/10.3153/fh21019.
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This study aimed to determine the effects of cooling on microbiological quality of lamb carcasses. Total Aerobic Bacteria Count (TABC), Enterobactericeae counts and the presence of Salmonella spp. were investigated in accordance with the Turkish Food Codex Regulation on Microbiological Criteria and European Union Commission Regulation 2073/2005. Sampling was carried out on aseptic conditions from the surface of 25 randomly selected lambs brought to slaughterhouse. The results showed that the mean TABC were 2.24±0.087 log cfu/cm2 before cooling and 2.41±0.061 log cfu/cm2 after cooling (P>0.05). The mean Enterobactericeae count was 0.21±0.11 log cfu/cm2 before cooling and 0.69±0.13 log cfu/cm2 after cooling (P<0.01). Furthermore, Salmonella spp. were determined on 3 carcasses before cooling and one another carcass after cooling. The influence of cooling on categorisation according to the legislations presented that it could potentially improve the numbers of acceptable carcasses for TABC and Salmonella spp. Nevertheless, the results exhibited that the hygiene and cooling stage of the slaughter line must be re-evaluated in terms of HACCP requirements and that corrective measures/actions must be taken.
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Mead, G. C., W. R. Hudson, and M. H. Hinton. "Effect of changes in processing to improve hygiene control on contamination of poultry carcasses with campylobacter." Epidemiology and Infection 115, no. 3 (December 1995): 495–500. http://dx.doi.org/10.1017/s0950268800058659.
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SummaryExamination of neck skin and caecal samples taken at a commercial processing plant from 15 randomly chosen poultry flocks showed that all flocks were contaminated initially with thermophilicCampylobacterspp., even in the apparent absence of caecal carriage. During processing, numbers of campylobacter on skin samples were reduced by between 10 and 1000-fold.To improve hygiene control generally, chlorinated-water sprays were used to limit microbial contamination on equipment and working surfaces. In addition, chlorine concentrations in process water were increased and any unnecessary carcass contact surfaces in the processing plant were removed. When comparing flocks before and after the changes, it was found that numbers of campylobacter on packaged carcasses were significantly lower after the changes had been made (P0·001). In practice, however, the reduction would be likely to have little impact on consumer exposure to campylobacter infection.
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YU, SHEW-LING, DECLAN BOLTON, CHERYL LAUBACH, PATRICIA KLINE, ALAN OSER, and SAMUEL A. PALUMBO. "Effect of Dehairing Operations on Microbiological Quality of Swine Carcasses†." Journal of Food Protection 62, no. 12 (December 1, 1999): 1478–81. http://dx.doi.org/10.4315/0362-028x-62.12.1478.
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To develop a hazard analysis and critical control point plan for food processing operations, critical control points must be determined. Swine slaughtering and dressing operations were investigated to establish their critical control points. We monitored the microbiology of swine carcasses by surface swabbing carcass bellies at various steps during the process and by quantitating total aerobic plate count (APC) and coliforms. Starting with a dehaired carcass, the sequential steps monitored included presingeing, postsingeing, polishing, and chilling. Initial results indicate that singeing and chilling substantially reduced the levels of APC and coliforms, whereas polishing increased their levels. The hygienic characteristics of individual operations involved in dressing swine carcasses were then evaluated in the second experiment. A set of 40 randomly selected carcasses leaving singeer, polisher, shaver, and washer were sampled. Carcasses were heavily contaminated during the final polishing procedure, and the APC increased threefold compared with prepolishing levels. Washing reduced the bacterial numbers by 69%. To reduce the microbial load on swine carcasses, final polishing and manual shaving steps were not used during the dressing operation on a set of 90 carcasses. APCs on singed carcasses were reduced from 1.34 to −0.15 log10 CFU/cm2 when the final polisher and manual shavers were not used. However, carcasses were subsequently recontaminated with bacteria after evisceration, and the APCs were similar (P > 0.05) regardless of whether the final polishing and manual shaving steps were used, averaging 1.30 and 1.46 log10 CFU/cm2. These results indicated that individual operations can be identified as critical control points, appropriate limits can be set and monitored in a hazard analysis and critical control point system, and steps where further changes to reduce bacterial levels may be needed for swine slaughtering plants.
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ZWEIFEL, C., and R. STEPHAN. "Microbiological Monitoring of Sheep Carcass Contamination in Three Swiss Abattoirs." Journal of Food Protection 66, no. 6 (June 1, 2003): 946–52. http://dx.doi.org/10.4315/0362-028x-66.6.946.
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At three Swiss abattoirs, 580 sheep carcasses were examined at 10 sites by the wet-dry double-swab technique. The aim of this study was to obtain data on microbiological contamination at the abattoirs and to develop a procedure for monitoring slaughter hygiene. Median aerobic plate counts (APCs) (log CFU/cm2) ranged from 2.5 to 3.8, with the brisket and neck sites showing the most extensive contamination. Enterobacteriaceae were detected on 68.1% of the carcasses and in 15.2% of the samples. The proportion of positive results ranged from 2.6% (for the hind leg and the flank at abattoir C) to 42.2% (for the perineal area at abattoir A). The percentage of samples testing positive for stx genes by polymerase chain reaction was 36.6%. A significant relationship between APC and the detection of Shiga toxin–producing Escherichia coli (STEC) was found for abattoirs A and B (depending on sampling site), whereas a significant relationship between Enterobacteriaceae and STEC detection was confirmed only for abattoir A (P < 0.05). In 57.1% of the 56 isolated non-O157 strains, stx2 genes were detected, and most of them were stx2d positive. Additional virulence factors were detected in 50% of the STEC strains, with 8.9% of these strains being eae positive, 50% being EHEC-hlyA positive, and 3.6% being astA positive. For the determination of carcass contamination, the monthly examination of 10 sheep carcasses for APC and Enterobacteriaceae counts in the neck, brisket, and perineal areas is recommended. This procedure is a valuable tool for the verification of slaughter hygiene according to hazard analysis critical control point principles.
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O'BRIEN, S. B., M. LENAHAN, T. SWEENEY, and J. J. SHERIDAN. "Assessing the Hygiene of Pig Carcasses Using Whole-Body Carcass Swabs Compared with the Four-Site Method in EC Decision 471." Journal of Food Protection 70, no. 2 (February 1, 2007): 432–39. http://dx.doi.org/10.4315/0362-028x-70.2.432.
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An investigation was carried out in a pig abattoir to determine the microbiological status of carcasses being produced after slaughter and dressing. The carcasses were sampled in accordance with EC Decision 471 in relation to the application of hazard analysis critical control point (HACCP) criteria to the slaughter of animals. In this regard, four sites on the animals were examined on five consecutive carcasses during each of 10 visits for the presence of total viable counts and Enterobacteriaceae. A comparison of the EC four-site method, with a whole-body swab technique, as a means of measuring carcass contamination found that the two methods gave significantly different results for both groups of organisms. A comparison of the mean of the individual data from the four sites with the data from the pooled samples revealed that there was a poor relationship between the two. Samples may be taken by excision or swabbing and allocated to three categories of process control, which, in turn, are based on microbiological criteria that are different, depending on whether sampling is by excision or swabbing. The influence of these changes in microbiological criteria is discussed in relation to the categorization of samples as acceptable, marginal, or unacceptable and the influence this has on process control. Finally, the proposed introduction of Salmonella as a safety indicator in the EC HACCP system is discussed.